RLG00000004373

High-affinity nitrate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0018637
Physical Location & Seq
Forward (+)
58802730 .. 58803524
795 bp
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UTR
Exon/CDS
Intron
RLM00000004373

Sequence Viewer

Length: 675 bp
ATGACATGGATTTTCTTAGCTTCTCTTCTTCTTCTCTCAAGCTTAGCAGAAACCTCTTATGGAGGCATTCTATTATCCTCTCTGCCCAACACTCTTGATGTCGCTGCTTCCCCTCACCATCCAGACCAGTGTAAGTATTTTCTCTGTTTTTCTTTTGCTGGAGTTAACACACCCAACGGGACATGGTATATGTCGATTAATATAAAAGCCGGAGAAGATAAACTCGGCGTTAGATGGTCTCTGAATAATAGGACTTTCCCAGTTGGAACCGATGCTGCGTATAAGACGGTCACCGTGAAGCTTTGCTACGCGCCGAGGAGTCAAGTGGGCCGGCCGGAGAGGAAGACGGAGGACGACCTCGACAAGGACAAGACATGCCAGATAGAGTTCTATACTGGACACTACAACCCCTCTAGAAAGTGGCCGTACTACGAGTCCACATTGGATGGCCACGTGCCGGCCGCCTTGTACTTCCTACGCGCCTACGCAAACGACTCCGCCGGAGTTGTGGTGGCTTATGGTGAAAGCACGGGACCGGACAGAGACTACAACATATTTGAGGTCGACGCAACTGAGCCAACAGAGGCAAGCAGCGGAAGCCGCAAGACGCTCGATATTTGCTTCGGTTCTTTTCCGCTTTGCTGTTGGTTGGGTTTTTTGTTGCTTAGAAGATAA

Protein Analysis

225

Amino Acids

24.99

Weight (kDa)

5.81

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAR2 PF16974 26 - 190 9e-39 High-affinity nitrate transporter accessory, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018637)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0192381
rosa_laevigata RLG00000004373
rosa_multiflora Rmu_co8086522.1_g000001 Rmu_sc0003108.1_g000007
rosa_roxburghii Rroxscaffold_3G00263080
rosa_rugosa Rorug07G0000800
rosa_samantha Rh7BG127100 Rh7CG128900 Rh7DG128900
rosa_wichuraiana Rw7G010740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 564
AccII CGCG 2 cut(s) 311, 480
AciI CCGC 5 cut(s) 462, 498, 594, 601, 635
AcoI YGGCCR 4 cut(s) 332, 422, 448, 459
AcvI CACGTG 1 cut(s) 454
AfaI GTAC 2 cut(s) 428, 470
AfiI CCNNNNNNNGG 2 cut(s) 364, 457
AluBI AGCT 3 cut(s) 20, 42, 301
AluI AGCT 3 cut(s) 20, 42, 301
Alw26I GTCTC 2 cut(s) 243, 537
AoxI GGCC 5 cut(s) 328, 332, 422, 448, 459
ApeKI GCWGC 3 cut(s) 104, 275, 591
AseI ATTAAT 1 cut(s) 198
AspLEI GCGC 2 cut(s) 313, 482
AspS9I GGNCC 2 cut(s) 328, 533
AsuHPI GGTGA 3 cut(s) 107, 283, 533
AvaII GGWCC 1 cut(s) 533
BalI TGGCCA 1 cut(s) 450
BarI GAAGNNNNNNTAC 2 cut(s) 290, 322
BbrPI CACGTG 1 cut(s) 454
BbsI GAAGAC 1 cut(s) 350
BbvI GCAGC 3 cut(s) 91, 262, 603
BccI CCATC 3 cut(s) 126, 228, 440
BceAI ACGGC 1 cut(s) 409
BcgI CGANNNNNNTGC 2 cut(s) 592, 626
BcoDI GTCTC 2 cut(s) 243, 537
BfaI CTAG 1 cut(s) 414
BisI GCNGC 5 cut(s) 105, 276, 462, 592, 601
BlpI GCTNAGC 1 cut(s) 43
BlsI GCNGC 5 cut(s) 106, 277, 463, 593, 602
Bme18I GGWCC 1 cut(s) 533
BmgT120I GGNCC 2 cut(s) 328, 533
BmiI GGNNCC 2 cut(s) 268, 534
BmrI ACTGGG 1 cut(s) 254
BmsI GCATC 1 cut(s) 262
BmuI ACTGGG 1 cut(s) 254
BpiI GAAGAC 1 cut(s) 350
BpmI CTGGAG 1 cut(s) 180
Bpu1102I GCTNAGC 1 cut(s) 43
BpuEI CTTGAG 1 cut(s) 22
BsaAI YACGTR 1 cut(s) 454
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 1 cut(s) 314
BsaWI WCCGGW 1 cut(s) 535
BsaXI ACNNNNNCTCC 2 cut(s) 495, 525
Bsc4I CCNNNNNNNGG 2 cut(s) 364, 457
Bse118I RCCGGY 2 cut(s) 330, 457
Bse1I ACTGG 3 cut(s) 127, 260, 400
BseDI CCNNGG 1 cut(s) 314
BseGI GGATG 2 cut(s) 118, 451
BseLI CCNNNNNNNGG 2 cut(s) 364, 457
BseMII CTCAG 1 cut(s) 564
BseNI ACTGG 3 cut(s) 127, 260, 400
BseRI GAGGAG 1 cut(s) 331
BseX3I CGGCCG 2 cut(s) 332, 459
BseXI GCAGC 3 cut(s) 91, 262, 603
Bsh1236I CGCG 2 cut(s) 311, 480
Bsh1285I CGRYCG 2 cut(s) 335, 462
BshFI GGCC 5 cut(s) 330, 334, 424, 450, 461
BsiEI CGRYCG 2 cut(s) 335, 462
BsiSI CCGG 6 cut(s) 210, 331, 335, 458, 501, 536
BslFI GGGAC 2 cut(s) 193, 546
BslI CCNNNNNNNGG 2 cut(s) 364, 457
BsmAI GTCTC 2 cut(s) 243, 537
BsmFI GGGAC 2 cut(s) 193, 546
BsmI GAATGC 1 cut(s) 66
BsnI GGCC 5 cut(s) 330, 334, 424, 450, 461
Bso31I GGTCTC 1 cut(s) 243
Bsp1720I GCTNAGC 1 cut(s) 43
BspACI CCGC 5 cut(s) 462, 498, 594, 601, 635
BspANI GGCC 5 cut(s) 330, 334, 424, 450, 461
BspCNI CTCAG 1 cut(s) 565
BspFNI CGCG 2 cut(s) 311, 480
BspLI GGNNCC 2 cut(s) 268, 534
BspTNI GGTCTC 1 cut(s) 243
BsrFI RCCGGY 2 cut(s) 330, 457
BsrI ACTGG 3 cut(s) 127, 260, 400
BssAI RCCGGY 2 cut(s) 330, 457
BssECI CCNNGG 1 cut(s) 314
Bst4CI ACNGT 2 cut(s) 289, 295
Bst6I CTCTTC 1 cut(s) 30
BstBAI YACGTR 1 cut(s) 454
BstC8I GCNNGC 3 cut(s) 332, 459, 589
BstDEI CTNAG 4 cut(s) 16, 43, 573, 665
BstEII GGTNACC 1 cut(s) 289
BstENI CCTNNNNNAGG 1 cut(s) 362
BstF5I GGATG 2 cut(s) 118, 451
BstFNI CGCG 2 cut(s) 311, 480
BstHHI GCGC 2 cut(s) 313, 482
BstMAI GTCTC 2 cut(s) 243, 537
BstMCI CGRYCG 2 cut(s) 335, 462
BstMWI GCNNNNNNNGC 2 cut(s) 597, 600
BstNSI RCATGY 1 cut(s) 378
BstPI GGTNACC 1 cut(s) 289
BstUI CGCG 2 cut(s) 311, 480
BstV1I GCAGC 3 cut(s) 91, 262, 603
BstV2I GAAGAC 1 cut(s) 350
BstZI CGGCCG 2 cut(s) 332, 459
BsuRI GGCC 5 cut(s) 330, 334, 424, 450, 461
BtsCI GGATG 2 cut(s) 118, 451
BtsIMutI CAGTG 1 cut(s) 134
Cac8I GCNNGC 3 cut(s) 332, 459, 589
CfoI GCGC 2 cut(s) 313, 482
Cfr10I RCCGGY 2 cut(s) 330, 457
Cfr13I GGNCC 2 cut(s) 328, 533
CseI GACGC 2 cut(s) 575, 616
Csp6I GTAC 2 cut(s) 427, 469
CviAII CATG 3 cut(s) 6, 183, 375
CviQI GTAC 2 cut(s) 427, 469
DdeI CTNAG 4 cut(s) 16, 43, 573, 665
EaeI YGGCCR 4 cut(s) 332, 422, 448, 459
EagI CGGCCG 2 cut(s) 332, 459
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
EciI GGCGGA 1 cut(s) 487
EclXI CGGCCG 2 cut(s) 332, 459
Eco31I GGTCTC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 533
Eco52I CGGCCG 2 cut(s) 332, 459
Eco72I CACGTG 1 cut(s) 454
Eco91I GGTNACC 1 cut(s) 289
EcoNI CCTNNNNNAGG 1 cut(s) 362
EcoO65I GGTNACC 1 cut(s) 289
FaeI CATG 3 cut(s) 9, 186, 378
FaqI GGGAC 2 cut(s) 193, 546
FatI CATG 3 cut(s) 5, 182, 374
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 5 cut(s) 105, 276, 462, 592, 601
FokI GGATG 2 cut(s) 105, 458
FseI GGCCGGCC 1 cut(s) 334
Fsp4HI GCNGC 5 cut(s) 105, 276, 462, 592, 601
FspBI CTAG 1 cut(s) 414
GlaI GCGC 2 cut(s) 312, 481
GluI GCNGC 5 cut(s) 105, 276, 462, 592, 601
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 5 cut(s) 330, 334, 424, 450, 461
HapII CCGG 6 cut(s) 210, 331, 335, 458, 501, 536
HgaI GACGC 2 cut(s) 575, 616
HhaI GCGC 2 cut(s) 313, 482
Hin1II CATG 3 cut(s) 9, 186, 378
Hin6I GCGC 2 cut(s) 311, 480
HinP1I GCGC 2 cut(s) 311, 480
HincII GTYRAC 2 cut(s) 166, 565
HindII GTYRAC 2 cut(s) 166, 565
HindIII AAGCTT 2 cut(s) 40, 299
HinfI GANTC 3 cut(s) 319, 434, 494
HpaI GTTAAC 1 cut(s) 166
HpaII CCGG 6 cut(s) 210, 331, 335, 458, 501, 536
HphI GGTGA 3 cut(s) 107, 283, 533
Hpy166II GTNNAC 3 cut(s) 166, 438, 565
Hpy188I TCNGA 1 cut(s) 243
Hpy188III TCNNGA 3 cut(s) 95, 122, 414
Hpy8I GTNNAC 3 cut(s) 166, 438, 565
Hpy99I CGWCG 1 cut(s) 569
HpyCH4III ACNGT 2 cut(s) 289, 295
HpyCH4IV ACGT 1 cut(s) 453
HpyF10VI GCNNNNNNNGC 2 cut(s) 597, 600
HpyF3I CTNAG 4 cut(s) 16, 43, 573, 665
HpySE526I ACGT 1 cut(s) 453
Hsp92II CATG 3 cut(s) 9, 186, 378
HspAI GCGC 2 cut(s) 311, 480
KroI GCCGGC 2 cut(s) 330, 457
KroNI GCCGGC 2 cut(s) 332, 459
KspAI GTTAAC 1 cut(s) 166
Lsp1109I GCAGC 3 cut(s) 91, 262, 603
LweI GCATC 1 cut(s) 262
MaeI CTAG 1 cut(s) 414
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 1 cut(s) 289
MboII GAAGA 5 cut(s) 17, 20, 23, 227, 355
MlsI TGGCCA 1 cut(s) 450
MluNI TGGCCA 1 cut(s) 450
MlyI GAGTC 3 cut(s) 328, 443, 488
MmeI TCCRAC 1 cut(s) 244
Mox20I TGGCCA 1 cut(s) 450
MroNI GCCGGC 2 cut(s) 330, 457
MscI TGGCCA 1 cut(s) 450
MseI TTAA 2 cut(s) 165, 198
Msp20I TGGCCA 1 cut(s) 450
MspA1I CMGCKG 1 cut(s) 594
MspI CCGG 6 cut(s) 210, 331, 335, 458, 501, 536
Mva1269I GAATGC 1 cut(s) 66
MvnI CGCG 2 cut(s) 311, 480
MwoI GCNNNNNNNGC 2 cut(s) 597, 600
NaeI GCCGGC 2 cut(s) 332, 459
NgoMIV GCCGGC 2 cut(s) 330, 457
NlaIII CATG 3 cut(s) 9, 186, 378
NlaIV GGNNCC 2 cut(s) 268, 534
NmeAIII GCCGAG 2 cut(s) 204, 339
NmuCI GTSAC 1 cut(s) 289
NspI RCATGY 1 cut(s) 378
PctI GAATGC 1 cut(s) 66
PdiI GCCGGC 2 cut(s) 332, 459
PkrI GCNGC 5 cut(s) 106, 277, 463, 593, 602
PleI GAGTC 3 cut(s) 327, 442, 488
PmaCI CACGTG 1 cut(s) 454
PmlI CACGTG 1 cut(s) 454
PpsI GAGTC 3 cut(s) 327, 442, 488
Ppu21I YACGTR 1 cut(s) 454
PshBI ATTAAT 1 cut(s) 198
PspCI CACGTG 1 cut(s) 454
PspEI GGTNACC 1 cut(s) 289
PspN4I GGNNCC 2 cut(s) 268, 534
PspPI GGNCC 2 cut(s) 328, 533
RigI GGCCGGCC 1 cut(s) 334
RsaI GTAC 2 cut(s) 428, 470
RsaNI GTAC 2 cut(s) 427, 469
SalI GTCGAC 1 cut(s) 563
SaqAI TTAA 2 cut(s) 165, 198
SatI GCNGC 5 cut(s) 105, 276, 462, 592, 601
Sau96I GGNCC 2 cut(s) 328, 533
SchI GAGTC 3 cut(s) 328, 443, 488
SetI ASST 7 cut(s) 22, 44, 56, 303, 360, 456, 564
SfaNI GCATC 1 cut(s) 262
SinI GGWCC 1 cut(s) 533
SmlI CTYRAG 1 cut(s) 37
SmoI CTYRAG 1 cut(s) 37
SsiI CCGC 5 cut(s) 462, 498, 594, 601, 635
SspMI CTAG 1 cut(s) 414
TaaI ACNGT 2 cut(s) 289, 295
TaiI ACGT 1 cut(s) 456
TaqI TCGA 4 cut(s) 194, 360, 564, 612
TatI WGTACW 1 cut(s) 468
TauI GCSGC 2 cut(s) 464, 603
Tru1I TTAA 2 cut(s) 165, 198
Tru9I TTAA 2 cut(s) 165, 198
TscAI CASTG 1 cut(s) 134
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 3 cut(s) 104, 275, 591
Tsp45I GTSAC 1 cut(s) 289
TspGWI ACGGA 1 cut(s) 362
TspRI CASTG 1 cut(s) 134
VpaK11BI GGWCC 1 cut(s) 533
VspI ATTAAT 1 cut(s) 198
XagI CCTNNNNNAGG 1 cut(s) 362
XbaI TCTAGA 1 cut(s) 413
XceI RCATGY 1 cut(s) 378
XmiI GTMKAC 1 cut(s) 564
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.