Rh7DG128900

High-affinity nitrate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0018637
Physical Location & Seq
Reverse (-)
10828000 .. 10828485
486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG128900.1

Sequence Viewer

Length: 486 bp
ATGTCGAGTAATATAAAAGCCGGAGAAGATAAACTCGGCGTTAGATGGTCTCTGAATAATAGGACTTTCCCAGTTGGAACCGATGCGGTGTATAAGACGGTCACCGTGAAGCTTTGCTACGCGCCGACGAGTCAAGTGGGCCGGCCAGAGAGGAAGACGGAGGACGACCTCGACAAGGACCAGACATGCCAGATAGAGTTCTATACTGGACACTACAACCGGTCTAGCAGGTGGCCGTACTACGAGTCGACATTGGATGGCCACGTGCCGGCCGCCTTGTACTTCCTACGCGCCTACGCAAACGACTCCGCCGGAGTTGTGGTGGCTTATGGTGAAAGCACGGGACCGGACAGAGACTACAACATATTTGAGGTCGACGCAACTGAGCCAACAGAGGCAAGCAGCGGAAGCCGCAAGACGCTCGATAATATTTGCTTCGGTTCTTTCTCCGCTTTGCTGTTGGTTGGGTTTTTTGTTGCCTATTAA

Protein Analysis

161

Amino Acids

17.91

Weight (kDa)

5.0

Isoelectric Point (pI)

57.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAR2 PF16974 4 - 127 2.2e-36 High-affinity nitrate transporter accessory, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018637)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0192381
rosa_laevigata RLG00000004373
rosa_multiflora Rmu_co8086522.1_g000001 Rmu_sc0003108.1_g000007
rosa_roxburghii Rroxscaffold_3G00263080
rosa_rugosa Rorug07G0000800
rosa_samantha Rh7BG127100 Rh7CG128900 Rh7DG128900
rosa_wichuraiana Rw7G010740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 219
Acc36I ACCTGC 1 cut(s) 219
AccI GTMKAC 2 cut(s) 248, 375
AccII CGCG 2 cut(s) 122, 291
AciI CCGC 6 cut(s) 86, 273, 309, 405, 412, 450
AcoI YGGCCR 4 cut(s) 143, 233, 259, 270
AcvI CACGTG 1 cut(s) 265
AfaI GTAC 2 cut(s) 239, 281
AfiI CCNNNNNNNGG 2 cut(s) 175, 268
AgeI ACCGGT 1 cut(s) 219
AluBI AGCT 1 cut(s) 112
AluI AGCT 1 cut(s) 112
Alw26I GTCTC 2 cut(s) 54, 348
AoxI GGCC 5 cut(s) 139, 143, 233, 259, 270
ApeKI GCWGC 1 cut(s) 402
AsiGI ACCGGT 1 cut(s) 219
AspLEI GCGC 2 cut(s) 124, 293
AspS9I GGNCC 3 cut(s) 139, 178, 344
AsuHPI GGTGA 2 cut(s) 94, 344
AvaII GGWCC 2 cut(s) 178, 344
BalI TGGCCA 1 cut(s) 261
BarI GAAGNNNNNNTAC 2 cut(s) 101, 133
BbrPI CACGTG 1 cut(s) 265
BbsI GAAGAC 1 cut(s) 161
BbvI GCAGC 1 cut(s) 414
BccI CCATC 2 cut(s) 39, 251
BceAI ACGGC 1 cut(s) 220
BcgI CGANNNNNNTGC 2 cut(s) 403, 437
BcoDI GTCTC 2 cut(s) 54, 348
BfaI CTAG 1 cut(s) 225
BfuAI ACCTGC 1 cut(s) 219
BisI GCNGC 3 cut(s) 273, 403, 412
BlsI GCNGC 3 cut(s) 274, 404, 413
Bme18I GGWCC 2 cut(s) 178, 344
BmgT120I GGNCC 3 cut(s) 139, 178, 344
BmiI GGNNCC 2 cut(s) 79, 345
BmrI ACTGGG 1 cut(s) 65
BmsI GCATC 1 cut(s) 73
BmuI ACTGGG 1 cut(s) 65
BpiI GAAGAC 1 cut(s) 161
BsaAI YACGTR 1 cut(s) 265
BsaI GGTCTC 1 cut(s) 54
BsaWI WCCGGW 2 cut(s) 219, 346
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 268
Bse118I RCCGGY 3 cut(s) 141, 219, 268
Bse1I ACTGG 2 cut(s) 71, 211
BseGI GGATG 1 cut(s) 262
BseLI CCNNNNNNNGG 2 cut(s) 175, 268
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 2 cut(s) 71, 211
BseX3I CGGCCG 1 cut(s) 270
BseXI GCAGC 1 cut(s) 414
Bsh1236I CGCG 2 cut(s) 122, 291
Bsh1285I CGRYCG 1 cut(s) 273
BshFI GGCC 5 cut(s) 141, 145, 235, 261, 272
BshTI ACCGGT 1 cut(s) 219
BsiEI CGRYCG 1 cut(s) 273
BsiSI CCGG 6 cut(s) 21, 142, 220, 269, 312, 347
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 2 cut(s) 175, 268
BsmAI GTCTC 2 cut(s) 54, 348
BsmFI GGGAC 1 cut(s) 357
BsnI GGCC 5 cut(s) 141, 145, 235, 261, 272
Bso31I GGTCTC 1 cut(s) 54
BspACI CCGC 6 cut(s) 86, 273, 309, 405, 412, 450
BspANI GGCC 5 cut(s) 141, 145, 235, 261, 272
BspCNI CTCAG 1 cut(s) 376
BspFNI CGCG 2 cut(s) 122, 291
BspLI GGNNCC 2 cut(s) 79, 345
BspMI ACCTGC 1 cut(s) 219
BspTNI GGTCTC 1 cut(s) 54
BsrFI RCCGGY 3 cut(s) 141, 219, 268
BsrI ACTGG 2 cut(s) 71, 211
BssAI RCCGGY 3 cut(s) 141, 219, 268
Bst4CI ACNGT 2 cut(s) 100, 106
BstBAI YACGTR 1 cut(s) 265
BstC8I GCNNGC 3 cut(s) 143, 270, 400
BstDEI CTNAG 1 cut(s) 384
BstEII GGTNACC 1 cut(s) 100
BstENI CCTNNNNNAGG 1 cut(s) 173
BstF5I GGATG 1 cut(s) 262
BstFNI CGCG 2 cut(s) 122, 291
BstHHI GCGC 2 cut(s) 124, 293
BstMAI GTCTC 2 cut(s) 54, 348
BstMCI CGRYCG 1 cut(s) 273
BstMWI GCNNNNNNNGC 2 cut(s) 408, 411
BstNSI RCATGY 1 cut(s) 189
BstPI GGTNACC 1 cut(s) 100
BstUI CGCG 2 cut(s) 122, 291
BstV1I GCAGC 1 cut(s) 414
BstV2I GAAGAC 1 cut(s) 161
BstZI CGGCCG 1 cut(s) 270
BsuRI GGCC 5 cut(s) 141, 145, 235, 261, 272
BtsCI GGATG 1 cut(s) 262
BveI ACCTGC 1 cut(s) 219
Cac8I GCNNGC 3 cut(s) 143, 270, 400
CfoI GCGC 2 cut(s) 124, 293
Cfr10I RCCGGY 3 cut(s) 141, 219, 268
Cfr13I GGNCC 3 cut(s) 139, 178, 344
CseI GACGC 2 cut(s) 386, 427
Csp6I GTAC 2 cut(s) 238, 280
CspAI ACCGGT 1 cut(s) 219
CviAII CATG 1 cut(s) 186
CviQI GTAC 2 cut(s) 238, 280
DdeI CTNAG 1 cut(s) 384
EaeI YGGCCR 4 cut(s) 143, 233, 259, 270
EagI CGGCCG 1 cut(s) 270
EciI GGCGGA 1 cut(s) 298
EclXI CGGCCG 1 cut(s) 270
Eco31I GGTCTC 1 cut(s) 54
Eco47I GGWCC 2 cut(s) 178, 344
Eco52I CGGCCG 1 cut(s) 270
Eco72I CACGTG 1 cut(s) 265
Eco91I GGTNACC 1 cut(s) 100
EcoNI CCTNNNNNAGG 1 cut(s) 173
EcoO65I GGTNACC 1 cut(s) 100
FaeI CATG 1 cut(s) 189
FaiI YATR 6 cut(s) 14, 93, 187, 204, 330, 365
FaqI GGGAC 1 cut(s) 357
FatI CATG 1 cut(s) 185
FblI GTMKAC 2 cut(s) 248, 375
Fnu4HI GCNGC 3 cut(s) 273, 403, 412
FokI GGATG 1 cut(s) 269
FseI GGCCGGCC 1 cut(s) 145
Fsp4HI GCNGC 3 cut(s) 273, 403, 412
FspBI CTAG 1 cut(s) 225
GlaI GCGC 2 cut(s) 123, 292
GluI GCNGC 3 cut(s) 273, 403, 412
HaeIII GGCC 5 cut(s) 141, 145, 235, 261, 272
HapII CCGG 6 cut(s) 21, 142, 220, 269, 312, 347
HgaI GACGC 2 cut(s) 386, 427
HhaI GCGC 2 cut(s) 124, 293
Hin1II CATG 1 cut(s) 189
Hin6I GCGC 2 cut(s) 122, 291
HinP1I GCGC 2 cut(s) 122, 291
HincII GTYRAC 2 cut(s) 249, 376
HindII GTYRAC 2 cut(s) 249, 376
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 3 cut(s) 130, 245, 305
HpaII CCGG 6 cut(s) 21, 142, 220, 269, 312, 347
HphI GGTGA 2 cut(s) 94, 344
Hpy166II GTNNAC 2 cut(s) 249, 376
Hpy188I TCNGA 1 cut(s) 54
Hpy8I GTNNAC 2 cut(s) 249, 376
Hpy99I CGWCG 2 cut(s) 130, 380
HpyCH4III ACNGT 2 cut(s) 100, 106
HpyCH4IV ACGT 1 cut(s) 264
HpyF10VI GCNNNNNNNGC 2 cut(s) 408, 411
HpyF3I CTNAG 1 cut(s) 384
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 1 cut(s) 189
HspAI GCGC 2 cut(s) 122, 291
KroI GCCGGC 2 cut(s) 141, 268
KroNI GCCGGC 2 cut(s) 143, 270
Lsp1109I GCAGC 1 cut(s) 414
LweI GCATC 1 cut(s) 73
MaeI CTAG 1 cut(s) 225
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 1 cut(s) 100
MboII GAAGA 2 cut(s) 38, 166
MlsI TGGCCA 1 cut(s) 261
MluNI TGGCCA 1 cut(s) 261
MlyI GAGTC 3 cut(s) 139, 254, 299
MmeI TCCRAC 1 cut(s) 55
MnlI CCTC 5 cut(s) 144, 154, 179, 364, 388
Mox20I TGGCCA 1 cut(s) 261
MroNI GCCGGC 2 cut(s) 141, 268
MscI TGGCCA 1 cut(s) 261
MseI TTAA 1 cut(s) 484
Msp20I TGGCCA 1 cut(s) 261
MspA1I CMGCKG 1 cut(s) 405
MspI CCGG 6 cut(s) 21, 142, 220, 269, 312, 347
MvnI CGCG 2 cut(s) 122, 291
MwoI GCNNNNNNNGC 2 cut(s) 408, 411
NaeI GCCGGC 2 cut(s) 143, 270
NgoMIV GCCGGC 2 cut(s) 141, 268
NlaIII CATG 1 cut(s) 189
NlaIV GGNNCC 2 cut(s) 79, 345
NmeAIII GCCGAG 1 cut(s) 15
NmuCI GTSAC 1 cut(s) 100
NspI RCATGY 1 cut(s) 189
PaqCI CACCTGC 1 cut(s) 219
PdiI GCCGGC 2 cut(s) 143, 270
PinAI ACCGGT 1 cut(s) 219
PkrI GCNGC 3 cut(s) 274, 404, 413
PleI GAGTC 3 cut(s) 138, 253, 299
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 3 cut(s) 138, 253, 299
Ppu21I YACGTR 1 cut(s) 265
PspCI CACGTG 1 cut(s) 265
PspEI GGTNACC 1 cut(s) 100
PspN4I GGNNCC 2 cut(s) 79, 345
PspPI GGNCC 3 cut(s) 139, 178, 344
RigI GGCCGGCC 1 cut(s) 145
RsaI GTAC 2 cut(s) 239, 281
RsaNI GTAC 2 cut(s) 238, 280
SalI GTCGAC 2 cut(s) 247, 374
SaqAI TTAA 1 cut(s) 484
SatI GCNGC 3 cut(s) 273, 403, 412
Sau96I GGNCC 3 cut(s) 139, 178, 344
SchI GAGTC 3 cut(s) 139, 254, 299
SetI ASST 5 cut(s) 114, 171, 233, 267, 375
SfaNI GCATC 1 cut(s) 73
SinI GGWCC 2 cut(s) 178, 344
SsiI CCGC 6 cut(s) 86, 273, 309, 405, 412, 450
SspI AATATT 1 cut(s) 430
SspMI CTAG 1 cut(s) 225
TaaI ACNGT 2 cut(s) 100, 106
TaiI ACGT 1 cut(s) 267
TaqI TCGA 5 cut(s) 5, 171, 248, 375, 423
TatI WGTACW 1 cut(s) 279
TauI GCSGC 2 cut(s) 275, 414
Tru1I TTAA 1 cut(s) 484
Tru9I TTAA 1 cut(s) 484
TseFI GTSAC 1 cut(s) 100
TseI GCWGC 1 cut(s) 402
Tsp45I GTSAC 1 cut(s) 100
TspGWI ACGGA 1 cut(s) 173
VpaK11BI GGWCC 2 cut(s) 178, 344
XagI CCTNNNNNAGG 1 cut(s) 173
XceI RCATGY 1 cut(s) 189
XmiI GTMKAC 2 cut(s) 248, 375
XspI CTAG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.