RLG00000005949

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0018429
Physical Location & Seq
Forward (+)
2992785 .. 2995959
3175 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005949

Sequence Viewer

Length: 2511 bp
ATGGGACATCCATTTAGTCTCTCAAAGCTAGACTGGCTGCCAGATTTTCCTATTGATGCCATGAGATTCGCTTTGGCTTATGTTTGTGATGGTCCTGTTAATGCTGCAAAGATTAACTTTCTACATAGCAATGCAGATTCTGTATTTGATGCTGCATACGATGCCATCATGAGTCTCACTAAAGAGATGTCATGGCACGAAGAATTTTTGGCTGCAGATTTCGAGTATTGTTTGAGAACAGGTCCCCCTTCTACTTCTGCAGATAAAGTATTTGCCAATGACATAAACATTGCCTTGAACAAGACCAAACAGCATTACGAGGCTTGCCATTTTCGAGAAGCCCTCAAGTCTGGCTTTCTAGCGCTTCAAGATGCCAGGGATTGGTACAAGAGTCTATGCGGTGGTACAAATGCAATGAACCGTGATTTGGTGTGGCGTTTTATGGAAATGCAGACGCGTCTTGTTGCTCCAATCCGTCCACATTATTCGGAATATGTGTGGAGAGAACTTTTGCACAATGTAAACTTTGTGGTGAAAGCAGACTGGCCTTCAGCCGATGCTCCAGAAAATTCAACCCTCCACAGCATCCATAAGTACTTGAAAAGCATAACTGATTCACTGAAAAAACAGATTGAAAGTGAGAGAACTAAGAAGGTATGCCCGAACATAGAAGTTTTAATATATGTCAAGGAGGAATCATTTAAATTAAAGATGAGGGAGGATGGGGAAGTTGAACTTGGGGCTCAAGCCTTAGATTTTAAGCTGCCATTTAGAGAGCTTAGGGTCCTTCTACAGAACTTGGACTTAATTAAGAGGCAAATCGGCGTTGAAAGGGTGCAAGTTCTGTCAGCTACCAACCCTCATGATCTGGCGAAAGCAGGCTCTCATGTTGAACTGATTAAGACTCTACGTCCAATACCTGGAAAGCCAATTAGTCTCATCTTGAAACGATCACGTATTTTAACAAGTATTACACAGTTAAAGCCATCCTCATCTGGTTGTTCGCCAATGCGCTCCAACACCACCATGTCCAAGCCAAAATGCAGTGATAAGAAAGCTAAACAGAAACATGGTGTGCGGGACTTACCAAACCAAGTAGCTAGTAAATGCAGTAATGAGGATATTCAAGTTCTTGAATGGTTTGATAAGCAAAGTAAACAGAGGCATGGTATAGGGGAAACGAAGAACCAATTTTCTACATCCACAAGAGACAACAAGTCTCGAGATGATTTACACTCTTATCCTCCATGTAAGTTAAGATTCAGGGGTTCTTTCGACAAGGCAATGGACGCTGCGTGTGATCAAGACAAGTGGCTCCTGGTGAACTTGCAATGCAGGGAGGATCTCAGCTCACATAAGCTTAATAGGGCTGACGAAGCCATCTTTCAGATCATTAGCACCAATTTCATCTTTTGGCAGGAGTATTCTGATTGGCCTGAAGGCACTATGGTTTCCAGCAACTACAAGTTGGATTCTAGAACTGTAGTGATCATTATTGACCCCGTCACCGGCCAAGCATTGCGTTCATGGAATGGGACTGTTCAACCGGACGATTTGCTGCTGGATCTATTACCGTTCCTGGATAGTAGCCCCAGGAATATGCATGTAGCCAAAGAGGAGGAGGAGGAACTGCGGCGCGCATTGGAAGCTTCAGTGGAAAGCATGAAGTTTGAGACTTTTGGAAGTACCTCCAAAGAGTATCTAGTACCGTCCTTCAATACTAGGTACATGACTACTAAAGCAACTGAAATAGATGAAGAGGAGGAACTGCAGCGTGCATTGGAAGCTTCGATGGAAAGCATAAAGTTTGAGACAAGTAATGCCAGTACCTCAAAAGAGGCTCTAGTTCCGTCCTTCAATACTAGCCTCATTAGTACTAAAGTAAGTGAAGAAGAAGAGGAACGGCTGCACGCATTGGAAGCTTTTGTGGAAAGGATGAAGTTGGAGACAAGTACTGCAAGTACCTCCAAAGGAGATGCAGATGGTGGAAGTACCTCCAAAGAGGATCCTCTAGAGATCTTCAATATTAAACCCATAATTACTTATGTAACTGAAGATGACGAGGAGGAATCTCGGTGTGCAATGGCAGATTTCGTGAATTCGTTGGAGACAAGTAGTGCAAGTACCTGTAAAGTCCCGACGTATAATAATGCTACTAGAGAAGAAGAGGATGAGGAATTGCAGCGCGCATTGGTGGCTTCCTTGGAAAGCCTGAAGTCGGAGACAAGTAGTGCAAGTACCTCCAAGGTTTCCACGAATACTTATGTAACTAAAGAAGAAGAAGAAGAAGATGATGAACTGCTTCATGCATTGGAAGTGGAAAGCATGAAGTGGGGGACTAGTGGAAGTACCTCTAAAGATCTAGTGCCGTTCTCCAATAGTAGCTCCATGTATACTGATGTGACTAGAGATCAAGATGAGGAACTGTGTCGCGCATTAGCAGCGTCCATGGAAAGTTTCAAGGTGGAGACATGGACTAGAGGAAGAAGCTCGAAAGAGAGAGTCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

837

Amino Acids

94.69

Weight (kDa)

5.19

Isoelectric Point (pI)

48.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anticodon_1 PF08264 88 - 212 3e-14 Anticodon-binding domain of tRNA ligase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33710
rosa_chinensis RchiOBHm_Chr4g0443181
rosa_laevigata RLG00000005949
rosa_multiflora Rmu_sc0007968.1_g000005
rosa_roxburghii Rroxscaffold_5G00383780
rosa_rugosa Rorug04G0344700
rosa_samantha Rh4BG410900 Rh4CG426400 Rh4DG406000
rosa_wichuraiana Rw4G034430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 381, 920
AccI GTMKAC 2 cut(s) 2393, 2505
AccII CGCG 4 cut(s) 457, 1638, 2187, 2433
AciI CCGC 3 cut(s) 399, 1078, 1633
AclWI GGATC 4 cut(s) 1350, 1572, 2000, 2013
AcoI YGGCCR 1 cut(s) 1510
AcsI RAATTY 3 cut(s) 203, 568, 2098
AcuI CTGAAG 5 cut(s) 534, 1458, 1635, 2073, 2234
AfeI AGCGCT 1 cut(s) 363
AfiI CCNNNNNNNGG 5 cut(s) 381, 427, 920, 1508, 2218
AflIII ACRYGT 1 cut(s) 455
AhdI GACNNNNNGTC 2 cut(s) 909, 1216
AhlI ACTAGT 1 cut(s) 2339
AjnI CCWGG 5 cut(s) 374, 919, 1317, 1578, 1592
AjuI GAANNNNNNNTTGG 4 cut(s) 410, 442, 720, 752
AlwI GGATC 4 cut(s) 1350, 1572, 2000, 2013
AlwNI CAGNNNCTG 1 cut(s) 140
Ama87I CYCGRG 1 cut(s) 1221
Aor51HI AGCGCT 1 cut(s) 363
AoxI GGCC 3 cut(s) 545, 1433, 1510
ApoI RAATTY 3 cut(s) 203, 568, 2098
Asp700I GAANNNNTTC 1 cut(s) 2301
AspLEI GCGC 7 cut(s) 364, 1014, 1638, 1640, 2187, 2189, 2435
AspS9I GGNCC 3 cut(s) 92, 242, 784
AsuHPI GGTGA 3 cut(s) 544, 1333, 1498
AvaI CYCGRG 1 cut(s) 1221
AvaII GGWCC 3 cut(s) 92, 242, 784
BaeI ACNNNNGTAYC 2 cut(s) 376, 409
BamHI GGATCC 1 cut(s) 2005
BanII GRGCYC 1 cut(s) 745
BccI CCATC 7 cut(s) 83, 173, 716, 994, 1388, 1786, 1976
BceAI ACGGC 2 cut(s) 1919, 2353
BciT130I CCWGG 5 cut(s) 376, 921, 1319, 1580, 1594
BclI TGATCA 2 cut(s) 1300, 1488
BcuI ACTAGT 1 cut(s) 2339
BfmI CTRYAG 5 cut(s) 213, 258, 791, 1482, 1769
BfoI RGCGCY 1 cut(s) 365
BglII AGATCT 2 cut(s) 2016, 2359
BmcAI AGTACT 3 cut(s) 596, 1876, 1954
Bme1390I CCNGG 5 cut(s) 376, 921, 1319, 1580, 1594
Bme18I GGWCC 3 cut(s) 92, 242, 784
BmeRI GACNNNNNGTC 2 cut(s) 909, 1216
BmeT110I CYCGRG 1 cut(s) 1221
BmgT120I GGNCC 3 cut(s) 92, 242, 784
BmiI GGNNCC 4 cut(s) 244, 785, 1316, 2007
BmrFI CCNGG 5 cut(s) 376, 921, 1319, 1580, 1594
BmsI GCATC 7 cut(s) 46, 139, 151, 361, 547, 594, 1966
BplI GAGNNNNNCTC 2 cut(s) 327, 359
BpmI CTGGAG 1 cut(s) 546
Bpu10I CCTNAGC 1 cut(s) 779
BpuEI CTTGAG 2 cut(s) 329, 729
BsaAI YACGTR 1 cut(s) 956
BsaJI CCNNGG 5 cut(s) 375, 1592, 2202, 2244, 2448
BsaWI WCCGGW 1 cut(s) 1546
Bsc4I CCNNNNNNNGG 5 cut(s) 381, 427, 920, 1508, 2218
Bse118I RCCGGY 1 cut(s) 1508
Bse1I ACTGG 3 cut(s) 38, 548, 1824
Bse3DI GCAATG 7 cut(s) 136, 288, 420, 1290, 1337, 1517, 2088
BseBI CCWGG 5 cut(s) 376, 921, 1319, 1580, 1594
BseDI CCNNGG 5 cut(s) 375, 1592, 2202, 2244, 2448
BseGI GGATG 7 cut(s) 7, 585, 727, 986, 1199, 1941, 2176
BseLI CCNNNNNNNGG 5 cut(s) 381, 427, 920, 1508, 2218
BseMI GCAATG 7 cut(s) 136, 288, 420, 1290, 1337, 1517, 2088
BseMII CTCAG 1 cut(s) 1360
BseNI ACTGG 3 cut(s) 38, 548, 1824
BsePI GCGCGC 2 cut(s) 1636, 2185
BseRI GAGGAG 5 cut(s) 1631, 1634, 1637, 1775, 2078
BsgI GTGCAG 1 cut(s) 1892
Bsh1236I CGCG 4 cut(s) 457, 1638, 2187, 2433
BshFI GGCC 3 cut(s) 547, 1435, 1512
BsiHKCI CYCGRG 1 cut(s) 1221
BsiSI CCGG 2 cut(s) 1509, 1547
BslFI GGGAC 6 cut(s) 18, 228, 1094, 1549, 2120, 2350
BslI CCNNNNNNNGG 5 cut(s) 381, 427, 920, 1508, 2218
BsmFI GGGAC 6 cut(s) 18, 228, 1094, 1549, 2120, 2350
BsnI GGCC 3 cut(s) 547, 1435, 1512
BsoBI CYCGRG 1 cut(s) 1221
Bsp1286I GDGCHC 1 cut(s) 745
Bsp19I CCATGG 1 cut(s) 2448
BspACI CCGC 3 cut(s) 399, 1078, 1633
BspANI GGCC 3 cut(s) 547, 1435, 1512
BspCNI CTCAG 1 cut(s) 1359
BspFNI CGCG 4 cut(s) 457, 1638, 2187, 2433
BspHI TCATGA 2 cut(s) 168, 862
BspLI GGNNCC 4 cut(s) 244, 785, 1316, 2007
BspMAI CTGCAG 3 cut(s) 217, 262, 1773
BspPI GGATC 4 cut(s) 1350, 1572, 2000, 2013
BsrDI GCAATG 7 cut(s) 136, 288, 420, 1290, 1337, 1517, 2088
BsrFI RCCGGY 1 cut(s) 1508
BsrI ACTGG 3 cut(s) 38, 548, 1824
BssAI RCCGGY 1 cut(s) 1508
BssECI CCNNGG 5 cut(s) 375, 1592, 2202, 2244, 2448
BssHII GCGCGC 2 cut(s) 1636, 2185
BssNAI GTATAC 1 cut(s) 2394
BssT1I CCWWGG 3 cut(s) 2202, 2244, 2448
Bst1107I GTATAC 1 cut(s) 2394
Bst2UI CCWGG 5 cut(s) 376, 921, 1319, 1580, 1594
Bst4CI ACNGT 7 cut(s) 422, 978, 1483, 1540, 1575, 1710, 2427
Bst6I CTCTTC 3 cut(s) 1752, 1890, 2160
BstAPI GCANNNNNTGC 1 cut(s) 161
BstBAI YACGTR 1 cut(s) 956
BstC8I GCNNGC 6 cut(s) 325, 880, 1638, 1776, 1911, 2187
BstDEI CTNAG 4 cut(s) 648, 751, 779, 1346
BstDSI CCRYGG 1 cut(s) 2448
BstF5I GGATG 7 cut(s) 7, 585, 727, 986, 1199, 1941, 2176
BstFNI CGCG 4 cut(s) 457, 1638, 2187, 2433
BstH2I RGCGCY 1 cut(s) 365
BstHHI GCGC 7 cut(s) 364, 1014, 1638, 1640, 2187, 2189, 2435
BstMWI GCNNNNNNNGC 9 cut(s) 34, 161, 1289, 1376, 1646, 1784, 1919, 2195, 2441
BstNI CCWGG 5 cut(s) 376, 921, 1319, 1580, 1594
BstNSI RCATGY 1 cut(s) 1607
BstSCI CCNGG 5 cut(s) 374, 919, 1317, 1578, 1592
BstSFI CTRYAG 5 cut(s) 213, 258, 791, 1482, 1769
BstUI CGCG 4 cut(s) 457, 1638, 2187, 2433
BstX2I RGATCY 5 cut(s) 1342, 1564, 2005, 2016, 2359
BstYI RGATCY 5 cut(s) 1342, 1564, 2005, 2016, 2359
BstZ17I GTATAC 1 cut(s) 2394
BsuRI GGCC 3 cut(s) 547, 1435, 1512
BtgI CCRYGG 1 cut(s) 2448
BtsCI GGATG 7 cut(s) 7, 585, 727, 986, 1199, 1941, 2176
BtsI GCAGTG 1 cut(s) 1051
BtsIMutI CAGTG 3 cut(s) 617, 1051, 1659
Cac8I GCNNGC 6 cut(s) 325, 880, 1638, 1776, 1911, 2187
CaiI CAGNNNCTG 1 cut(s) 140
CciI TCATGA 2 cut(s) 168, 862
CfoI GCGC 7 cut(s) 364, 1014, 1638, 1640, 2187, 2189, 2435
Cfr10I RCCGGY 1 cut(s) 1508
Cfr13I GGNCC 3 cut(s) 92, 242, 784
CseI GACGC 4 cut(s) 446, 463, 1298, 2433
CspCI CAANNNNNGTGG 2 cut(s) 1292, 1327
DdeI CTNAG 4 cut(s) 648, 751, 779, 1346
DraI TTTAAA 1 cut(s) 703
DriI GACNNNNNGTC 2 cut(s) 909, 1216
EaeI YGGCCR 1 cut(s) 1510
Eam1104I CTCTTC 3 cut(s) 1752, 1890, 2160
Eam1105I GACNNNNNGTC 2 cut(s) 909, 1216
EarI CTCTTC 3 cut(s) 1752, 1890, 2160
Eco130I CCWWGG 3 cut(s) 2202, 2244, 2448
Eco24I GRGCYC 1 cut(s) 745
Eco47I GGWCC 3 cut(s) 92, 242, 784
Eco47III AGCGCT 1 cut(s) 363
Eco57I CTGAAG 5 cut(s) 534, 1458, 1635, 2073, 2234
Eco88I CYCGRG 1 cut(s) 1221
EcoO109I RGGNCCY 2 cut(s) 242, 784
EcoRI GAATTC 1 cut(s) 2098
EcoRII CCWGG 5 cut(s) 374, 919, 1317, 1578, 1592
EcoT14I CCWWGG 3 cut(s) 2202, 2244, 2448
EcoT22I ATGCAT 2 cut(s) 1605, 2311
EcoT38I GRGCYC 1 cut(s) 745
ErhI CCWWGG 3 cut(s) 2202, 2244, 2448
FalI AAGNNNNNCTT 6 cut(s) 101, 133, 338, 370, 720, 752
FaqI GGGAC 6 cut(s) 18, 228, 1094, 1549, 2120, 2350
FauI CCCGC 1 cut(s) 1071
FbaI TGATCA 2 cut(s) 1300, 1488
FblI GTMKAC 2 cut(s) 2393, 2505
FokI GGATG 6 cut(s) 572, 734, 973, 1186, 1948, 2183
FriOI GRGCYC 1 cut(s) 745
GlaI GCGC 7 cut(s) 363, 1013, 1637, 1639, 2186, 2188, 2434
GsuI CTGGAG 1 cut(s) 546
HaeII RGCGCY 1 cut(s) 365
HaeIII GGCC 3 cut(s) 547, 1435, 1512
HapII CCGG 2 cut(s) 1509, 1547
HgaI GACGC 4 cut(s) 446, 463, 1298, 2433
HhaI GCGC 7 cut(s) 364, 1014, 1638, 1640, 2187, 2189, 2435
Hin6I GCGC 7 cut(s) 362, 1012, 1636, 1638, 2185, 2187, 2433
HinP1I GCGC 7 cut(s) 362, 1012, 1636, 1638, 2185, 2187, 2433
HincII GTYRAC 1 cut(s) 2506
HindII GTYRAC 1 cut(s) 2506
HindIII AAGCTT 4 cut(s) 1358, 1647, 1785, 1920
HpaII CCGG 2 cut(s) 1509, 1547
HphI GGTGA 3 cut(s) 544, 1333, 1498
Hpy166II GTNNAC 6 cut(s) 479, 523, 1157, 1324, 2394, 2506
Hpy188I TCNGA 4 cut(s) 490, 1389, 1429, 2221
Hpy8I GTNNAC 6 cut(s) 479, 523, 1157, 1324, 2394, 2506
Hpy99I CGWCG 1 cut(s) 2143
HpyAV CCTTC 7 cut(s) 258, 558, 646, 797, 1433, 1723, 1864
HpyCH4III ACNGT 7 cut(s) 422, 978, 1483, 1540, 1575, 1710, 2427
HpyCH4IV ACGT 3 cut(s) 910, 955, 2141
HpyF10VI GCNNNNNNNGC 9 cut(s) 34, 161, 1289, 1376, 1646, 1784, 1919, 2195, 2441
HpyF3I CTNAG 4 cut(s) 648, 751, 779, 1346
HpySE526I ACGT 3 cut(s) 910, 955, 2141
HspAI GCGC 7 cut(s) 362, 1012, 1636, 1638, 2185, 2187, 2433
Ksp22I TGATCA 2 cut(s) 1300, 1488
LmnI GCTCC 5 cut(s) 472, 565, 1019, 1320, 2390
LweI GCATC 7 cut(s) 46, 139, 151, 361, 547, 594, 1966
MaeII ACGT 3 cut(s) 910, 955, 2141
MaeIII GTNAC 4 cut(s) 1504, 2047, 2266, 2401
MflI RGATCY 5 cut(s) 1342, 1564, 2005, 2016, 2359
MhlI GDGCHC 1 cut(s) 745
MluI ACGCGT 1 cut(s) 455
MlyI GAGTC 4 cut(s) 181, 400, 898, 2511
MmeI TCCRAC 5 cut(s) 1041, 1449, 1923, 2085, 2199
Mph1103I ATGCAT 2 cut(s) 1605, 2311
MroXI GAANNNNTTC 1 cut(s) 2301
MslI CAYNNNNRTG 2 cut(s) 129, 1025
MspI CCGG 2 cut(s) 1509, 1547
MspR9I CCNGG 5 cut(s) 376, 921, 1319, 1580, 1594
MvaI CCWGG 5 cut(s) 376, 921, 1319, 1580, 1594
MvnI CGCG 4 cut(s) 457, 1638, 2187, 2433
MwoI GCNNNNNNNGC 9 cut(s) 34, 161, 1289, 1376, 1646, 1784, 1919, 2195, 2441
NcoI CCATGG 1 cut(s) 2448
NlaIV GGNNCC 4 cut(s) 244, 785, 1316, 2007
NmuCI GTSAC 2 cut(s) 1504, 2401
NsiI ATGCAT 2 cut(s) 1605, 2311
NspI RCATGY 1 cut(s) 1607
PacI TTAATTAA 1 cut(s) 810
PaeR7I CTCGAG 1 cut(s) 1221
PagI TCATGA 2 cut(s) 168, 862
PauI GCGCGC 2 cut(s) 1636, 2185
PdmI GAANNNNTTC 1 cut(s) 2301
PfeI GAWTC 7 cut(s) 66, 137, 614, 695, 1260, 1472, 2069
PflFI GACNNNGTC 1 cut(s) 1502
PflMI CCANNNNNTGG 2 cut(s) 381, 920
PfoI TCCNGGA 1 cut(s) 1578
PleI GAGTC 4 cut(s) 180, 399, 898, 2510
PpsI GAGTC 4 cut(s) 180, 399, 898, 2510
Ppu21I YACGTR 1 cut(s) 956
PpuMI RGGWCCY 2 cut(s) 242, 784
Psp5II RGGWCCY 2 cut(s) 242, 784
Psp6I CCWGG 5 cut(s) 374, 919, 1317, 1578, 1592
PspGI CCWGG 5 cut(s) 374, 919, 1317, 1578, 1592
PspN4I GGNNCC 4 cut(s) 244, 785, 1316, 2007
PspPI GGNCC 3 cut(s) 92, 242, 784
PspPPI RGGWCCY 2 cut(s) 242, 784
PstI CTGCAG 3 cut(s) 217, 262, 1773
PstNI CAGNNNCTG 1 cut(s) 140
PsuI RGATCY 5 cut(s) 1342, 1564, 2005, 2016, 2359
PsyI GACNNNGTC 1 cut(s) 1502
PteI GCGCGC 2 cut(s) 1636, 2185
RseI CAYNNNNRTG 2 cut(s) 129, 1025
SalI GTCGAC 1 cut(s) 2504
Sau96I GGNCC 3 cut(s) 92, 242, 784
ScaI AGTACT 3 cut(s) 596, 1876, 1954
SchI GAGTC 4 cut(s) 181, 400, 898, 2511
ScrFI CCNGG 5 cut(s) 376, 921, 1319, 1580, 1594
SduI GDGCHC 1 cut(s) 745
SfaNI GCATC 7 cut(s) 46, 139, 151, 361, 547, 594, 1966
SfcI CTRYAG 5 cut(s) 213, 258, 791, 1482, 1769
Sfr274I CTCGAG 1 cut(s) 1221
SinI GGWCC 3 cut(s) 92, 242, 784
SlaI CTCGAG 1 cut(s) 1221
SmiI ATTTAAAT 1 cut(s) 703
SmiMI CAYNNNNRTG 2 cut(s) 129, 1025
SmlI CTYRAG 3 cut(s) 344, 744, 1221
SmoI CTYRAG 3 cut(s) 344, 744, 1221
SpeI ACTAGT 1 cut(s) 2339
SsiI CCGC 3 cut(s) 399, 1078, 1633
SspI AATATT 1 cut(s) 2026
StyD4I CCNGG 5 cut(s) 374, 919, 1317, 1578, 1592
StyI CCWWGG 3 cut(s) 2202, 2244, 2448
SwaI ATTTAAAT 1 cut(s) 703
TaaI ACNGT 7 cut(s) 422, 978, 1483, 1540, 1575, 1710, 2427
TaiI ACGT 3 cut(s) 913, 958, 2144
TaqI TCGA 7 cut(s) 222, 334, 1222, 1275, 1790, 2492, 2505
TatI WGTACW 3 cut(s) 594, 1874, 1952
TauI GCSGC 1 cut(s) 1636
TfiI GAWTC 7 cut(s) 66, 137, 614, 695, 1260, 1472, 2069
TscAI CASTG 3 cut(s) 624, 1051, 1659
TseFI GTSAC 2 cut(s) 1504, 2401
Tsp45I GTSAC 2 cut(s) 1504, 2401
TspDTI ATGAA 9 cut(s) 431, 1396, 1515, 1679, 1770, 1952, 2294, 2310, 2342
TspGWI ACGGA 2 cut(s) 464, 1839
TspRI CASTG 3 cut(s) 624, 1051, 1659
Tth111I GACNNNGTC 1 cut(s) 1502
Van91I CCANNNNNTGG 2 cut(s) 381, 920
VpaK11BI GGWCC 3 cut(s) 92, 242, 784
XapI RAATTY 3 cut(s) 203, 568, 2098
XbaI TCTAGA 2 cut(s) 1475, 2011
XceI RCATGY 1 cut(s) 1607
XhoI CTCGAG 1 cut(s) 1221
XmiI GTMKAC 2 cut(s) 2393, 2505
XmnI GAANNNNTTC 1 cut(s) 2301
ZrmI AGTACT 3 cut(s) 596, 1876, 1954
Zsp2I ATGCAT 2 cut(s) 1605, 2311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.