Rh4CG426400

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0018429
Physical Location & Seq
Reverse (-)
66902364 .. 66906920
4557 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG426400.1

Sequence Viewer

Length: 2775 bp
ATGGCAGCCAAATGTGAAACAAGCTCATCCAAAAAGGCTAATAGAGACGTTCTCCTAGAGATTGAGGCAAAGGTTCGAAAATGTTGGGAGGATAAGAAAGTTCTCAGTTCTAAAAAGCCTGGCCACAAGTTCTTTGAAAAGTTCCCTTATAATTGTTTTATGGGACATCCATTTAGTCTCTCAAAGCTAGACTGGCTGCCAGATTTTCCTATTGATGCCATGAGATTCGCTTTGGCTTATGTTTGTGATGGTCCTGTTGATGCTGCAAAGATTAACTTTCTACATAGCAATGCAGATTCTGTATTTGATGCTGCATACGATGCCATCATAAGTCTCACTAAAGAGATGTCATGGCACGAAGAATTTTTGGCTGCAGATTTCGAGTATTGTTTGAGAACAGGTCCCCCCTCTACTTCTGCAGATAAGGTATTTGCCAATGACATAAACATTGCCTTGAACAAGACCAAACAGCATTACAAGGCTTGCCAGTTTCGAGAAGCCCTCGAGTCTGGCTTTCTAGCGCTTCAAGATGCCAGGGATTGGTACAAGAGTCTATGTGGTGGTACAAATGCAATGAACCGTGATTTGGTGTGGCGTTTTATGCAAATGCAGACGCGTCTTGTTGCTCCAATCTGTCCACATTATTCGGAATATGTGTGGAGAGAACTTTTGCACAATGTAAACTTTGTGGTGAAAGCAGACTGGCCTGCAGCCGATGCTCCAGAAAATTCAACCCTCCACAGTATCCATAAGTACTTGAAAAGCATAACTGATTCACTGAAAAGACAGATTGAAAGTGAGAGAACTAAGAAGGTATGCCCGAACATAGAAGTTTTAATATATGTCAAGGAGGAATCATTTAATTTAAAGATGAGGGAGGATGGGGAAGTTGAACTTGGAGCTCAAGCCTTAGATTTTATGCTGCCATTTAGAGAGCTTAGGGTCCTTCTACAGAACTTGGACTTAATTAAGAGGCAAATCGGCGTTGAAAGGGTGCAAGTTCTGTCAGCTACCAACCCTCATGATCTTGAGAAAGCGGGCTCTCATGTTGAACTGATTAAGACTCTACGTCCAATACCTGGAAAGCCAATTAGTCTCATCTTGAAACGATCACGTATTTTAACAAGTATTACACAGTTAAAGCCATCCTCATCTGGTTGTTCGCCAATGCGCTCCAACACCACCATGTCCAAGCCAAAATGCAGTGATAAGAAAGCCAAACAGAAACATGGTGTACGGGACTTACCAAACCAAGTAGCTAGTAAATGCAGCAATGAGGATATTCAAGTTCTTGAATGGTTTGATAAGCAAAGTAAACAGAGGCATGGTATAGAGGAAACGATGAACCAATTTTCTACATCCACAAGAGACAACAAGTCTCGAGATGATTTACACTCTTATCCTCCATGTAAGTTAAGATTCAAGGGTTCCTTCGACAAGGCAATGGACGCTGCGTGTGATCAAGACAAATGGCTCCTGGTGAACTTGCAATGCAGGGAGGATCTCAGCTCACATAAGCCTAATCGGGACATCTGGGCGGACAAAGACATCTCTCAGATCATTAGCACCAATTTCATCTTTTGGCAGGAGTACTCTGATTGGCCTGAAGGCACTATGGTTTCCAGCAACTACAAGTTGGATTCTAGAACTGTAGTGATCATCATTGACCCCGTCACCGGCCAAGCATTGCGTTCATGGAATAGGATGGTTCAACCGGACGATTTGCTGCTGGATCTATTACCGTTCCTGGATAGTAGCCCCAGGAATATGCATGTAGCCAAAGAGGAGGAGGAGCAACTGCGGCGCGCATTGGAAGCTTCGGTGGAAAGCGTGAAGTTTGAGACTTTTGGAAGAACCTCCAAAGAGTATCTAGTACCGTCCTTCAATACTAGACCCATGACTAACAAAGCAACTGAAATAGATGAAGAGGAGGAACTGCAGCGTGCATTGGAAGCTTCGATGGAAAGCATAAAGTTTGAGACAAGTAATGCCAGTACCTCAAAAGAGGCTCTAGTTCCGTCCTTCAATACTAGCCCCATTAGTACTAGAGTAAGTGAAGAAGAAGAGGAACGGCTGCACGCATTGGAAGCTTTTGTGGAAAGGATGAAGTTGGAGACAAGTACAGCAAGTACCTCCAAAGGAGATGCAGTGTCGTCCTTGAGTAGTAGCTCCAGGAATACTTATGTAACTAGACCAGAAGAAGAAGAAGAGGAACTGCGGCGCGCATTGGCAGCTTCCATGGAGTCGGATGGTGGAAGTACCTCCAAAGAGGACCCACTAGAGATCTTCAATATTAAACCCATAATTACTTATGTAACTGAAGATGACGAGGAGGAATCTCGGTGTGCAATGGCAGATTTCGTGAATTCGTTGGAGACAAGTAGTGCAAGTACGTCTAAAGTCCCGACGTTTAATAATGCTACTAGAGAAGAAGAGGATGAGGAATTGCAGCGCGCATTGGTGGCTTCCTTGGAAAGCCTGAAGTCGGAGACAAGTAGTGCAAGTACCTCCAAGGTTTCCAAGAATACTTATGTAACTAAAGAAGAAGAAGATGATGAACTGCTTCATGCATTGGAAGTGGAAAGCATGAAGTGGGGGACTAGTGGAAGTACCTCTAAAGAAGATCTAGTACCGTTCTCCATTAGTAGCTCCATGTATACTGATGTGACTAGAGACGAAGATGAGGAACTGTGTCGCACATTAGCAGCGTCCATGGAAAGTTTCAAGGTGGAGACCTGGACTAGAGGAAGAAGCGCGAAAGAGAGAGTCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

924

Amino Acids

104.95

Weight (kDa)

5.26

Isoelectric Point (pI)

51.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anticodon_1 PF08264 141 - 263 4.8e-14 Anticodon-binding domain of tRNA ligase
Thioredoxin_7 PF13899 477 - 530 6.8e-09 Thioredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33710
rosa_chinensis RchiOBHm_Chr4g0443181
rosa_laevigata RLG00000005949
rosa_multiflora Rmu_sc0007968.1_g000005
rosa_roxburghii Rroxscaffold_5G00383780
rosa_rugosa Rorug04G0344700
rosa_samantha Rh4BG410900 Rh4CG426400 Rh4DG406000
rosa_wichuraiana Rw4G034430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 150
AccB7I CCANNNNNTGG 2 cut(s) 540, 1079
AccI GTMKAC 2 cut(s) 2657, 2769
AccII CGCG 5 cut(s) 616, 1806, 2223, 2454, 2756
AciI CCGC 4 cut(s) 1037, 1538, 1801, 2218
AclWI GGATC 2 cut(s) 1509, 1740
AcoI YGGCCR 2 cut(s) 121, 1678
AcsI RAATTY 3 cut(s) 362, 727, 2365
AcuI CTGAAG 3 cut(s) 1626, 2340, 2501
AfeI AGCGCT 1 cut(s) 522
AfiI CCNNNNNNNGG 5 cut(s) 540, 586, 1079, 1676, 2485
AflIII ACRYGT 1 cut(s) 614
AhdI GACNNNNNGTC 2 cut(s) 1068, 1375
AhlI ACTAGT 1 cut(s) 2600
AjnI CCWGG 8 cut(s) 118, 533, 1078, 1476, 1746, 1760, 2171, 2735
AjuI GAANNNNNNNTTGG 4 cut(s) 569, 601, 879, 911
Alw21I GWGCWC 1 cut(s) 904
AlwI GGATC 2 cut(s) 1509, 1740
AlwNI CAGNNNCTG 1 cut(s) 299
Ama87I CYCGRG 2 cut(s) 503, 1380
Aor51HI AGCGCT 1 cut(s) 522
AoxI GGCC 4 cut(s) 121, 704, 1601, 1678
ApoI RAATTY 3 cut(s) 362, 727, 2365
Asp700I GAANNNNTTC 1 cut(s) 2562
AspLEI GCGC 9 cut(s) 523, 1173, 1806, 1808, 2223, 2225, 2454, 2456, 2756
AspS9I GGNCC 4 cut(s) 251, 401, 943, 2272
AsuHPI GGTGA 3 cut(s) 703, 1492, 1666
AsuII TTCGAA 1 cut(s) 76
AvaI CYCGRG 2 cut(s) 503, 1380
AvaII GGWCC 4 cut(s) 251, 401, 943, 2272
BaeI ACNNNNGTAYC 2 cut(s) 535, 568
BalI TGGCCA 1 cut(s) 123
BanII GRGCYC 2 cut(s) 904, 1043
BarI GAAGNNNNNNTAC 2 cut(s) 2613, 2645
Bbv12I GWGCWC 1 cut(s) 904
BccI CCATC 7 cut(s) 242, 332, 875, 1153, 1699, 1954, 2243
BceAI ACGGC 1 cut(s) 2087
BciT130I CCWGG 8 cut(s) 120, 535, 1080, 1478, 1748, 1762, 2173, 2737
BciVI GTATCC 1 cut(s) 755
BclI TGATCA 2 cut(s) 1459, 1656
BcuI ACTAGT 1 cut(s) 2600
BfmI CTRYAG 6 cut(s) 372, 417, 708, 950, 1650, 1937
BfoI RGCGCY 1 cut(s) 524
BfuI GTATCC 1 cut(s) 755
BglII AGATCT 2 cut(s) 2283, 2623
BmcAI AGTACT 3 cut(s) 755, 1592, 2044
Bme1390I CCNGG 8 cut(s) 120, 535, 1080, 1478, 1748, 1762, 2173, 2737
Bme18I GGWCC 4 cut(s) 251, 401, 943, 2272
BmeRI GACNNNNNGTC 2 cut(s) 1068, 1375
BmeT110I CYCGRG 2 cut(s) 503, 1380
BmgT120I GGNCC 4 cut(s) 251, 401, 943, 2272
BmiI GGNNCC 5 cut(s) 403, 944, 1429, 1475, 2274
BmrFI CCNGG 8 cut(s) 120, 535, 1080, 1478, 1748, 1762, 2173, 2737
BmsI GCATC 7 cut(s) 205, 250, 298, 310, 520, 706, 2134
BplI GAGNNNNNCTC 4 cut(s) 36, 68, 486, 518
BpmI CTGGAG 2 cut(s) 705, 2155
Bpu10I CCTNAGC 1 cut(s) 938
Bpu14I TTCGAA 1 cut(s) 76
BpuEI CTTGAG 3 cut(s) 888, 1049, 2179
BsaAI YACGTR 1 cut(s) 1115
BsaI GGTCTC 1 cut(s) 2726
BsaJI CCNNGG 6 cut(s) 534, 1760, 2238, 2469, 2511, 2712
BsaWI WCCGGW 1 cut(s) 1714
BsaXI ACNNNNNCTCC 2 cut(s) 2133, 2163
Bsc4I CCNNNNNNNGG 5 cut(s) 540, 586, 1079, 1676, 2485
Bse118I RCCGGY 1 cut(s) 1676
Bse1I ACTGG 4 cut(s) 197, 487, 707, 1992
Bse3DI GCAATG 8 cut(s) 295, 447, 579, 1279, 1449, 1496, 1685, 2355
BseBI CCWGG 8 cut(s) 120, 535, 1080, 1478, 1748, 1762, 2173, 2737
BseDI CCNNGG 6 cut(s) 534, 1760, 2238, 2469, 2511, 2712
BseGI GGATG 9 cut(s) 26, 166, 886, 1145, 1358, 1710, 2109, 2254, 2443
BseLI CCNNNNNNNGG 5 cut(s) 540, 586, 1079, 1676, 2485
BseMI GCAATG 8 cut(s) 295, 447, 579, 1279, 1449, 1496, 1685, 2355
BseMII CTCAG 3 cut(s) 118, 1519, 1568
BseNI ACTGG 4 cut(s) 197, 487, 707, 1992
BsePI GCGCGC 3 cut(s) 1804, 2221, 2452
BseRI GAGGAG 5 cut(s) 1799, 1802, 1805, 1943, 2345
BsgI GTGCAG 1 cut(s) 2060
Bsh1236I CGCG 5 cut(s) 616, 1806, 2223, 2454, 2756
BshFI GGCC 4 cut(s) 123, 706, 1603, 1680
BsiHKAI GWGCWC 1 cut(s) 904
BsiHKCI CYCGRG 2 cut(s) 503, 1380
BsiSI CCGG 2 cut(s) 1677, 1715
BslFI GGGAC 6 cut(s) 177, 387, 1253, 1541, 2387, 2611
BslI CCNNNNNNNGG 5 cut(s) 540, 586, 1079, 1676, 2485
BsmBI CGTCTC 2 cut(s) 39, 2667
BsmFI GGGAC 6 cut(s) 177, 387, 1253, 1541, 2387, 2611
BsnI GGCC 4 cut(s) 123, 706, 1603, 1680
Bso31I GGTCTC 1 cut(s) 2726
BsoBI CYCGRG 2 cut(s) 503, 1380
Bsp119I TTCGAA 1 cut(s) 76
Bsp1286I GDGCHC 2 cut(s) 904, 1043
Bsp143I GATC 9 cut(s) 1024, 1109, 1459, 1501, 1557, 1656, 1732, 2283, 2623
Bsp19I CCATGG 2 cut(s) 2238, 2712
BspACI CCGC 4 cut(s) 1037, 1538, 1801, 2218
BspANI GGCC 4 cut(s) 123, 706, 1603, 1680
BspCNI CTCAG 3 cut(s) 117, 1518, 1567
BspFNI CGCG 5 cut(s) 616, 1806, 2223, 2454, 2756
BspHI TCATGA 1 cut(s) 1021
BspLI GGNNCC 5 cut(s) 403, 944, 1429, 1475, 2274
BspMAI CTGCAG 4 cut(s) 376, 421, 712, 1941
BspPI GGATC 2 cut(s) 1509, 1740
BspT104I TTCGAA 1 cut(s) 76
BspTNI GGTCTC 1 cut(s) 2726
BsrDI GCAATG 8 cut(s) 295, 447, 579, 1279, 1449, 1496, 1685, 2355
BsrFI RCCGGY 1 cut(s) 1676
BsrI ACTGG 4 cut(s) 197, 487, 707, 1992
BssAI RCCGGY 1 cut(s) 1676
BssECI CCNNGG 6 cut(s) 534, 1760, 2238, 2469, 2511, 2712
BssHII GCGCGC 3 cut(s) 1804, 2221, 2452
BssMI GATC 9 cut(s) 1024, 1109, 1459, 1501, 1557, 1656, 1732, 2283, 2623
BssNAI GTATAC 1 cut(s) 2658
BssT1I CCWWGG 4 cut(s) 2238, 2469, 2511, 2712
Bst1107I GTATAC 1 cut(s) 2658
Bst2UI CCWGG 8 cut(s) 120, 535, 1080, 1478, 1748, 1762, 2173, 2737
Bst4CI ACNGT 8 cut(s) 581, 743, 1137, 1651, 1743, 1878, 2634, 2691
Bst6I CTCTTC 4 cut(s) 1920, 2058, 2202, 2427
BstAPI GCANNNNNTGC 2 cut(s) 320, 716
BstBAI YACGTR 1 cut(s) 1115
BstBI TTCGAA 1 cut(s) 76
BstC8I GCNNGC 8 cut(s) 484, 708, 1039, 1806, 1944, 2079, 2223, 2454
BstDEI CTNAG 6 cut(s) 104, 807, 910, 938, 1505, 1554
BstDSI CCRYGG 2 cut(s) 2238, 2712
BstF5I GGATG 9 cut(s) 26, 166, 886, 1145, 1358, 1710, 2109, 2254, 2443
BstFNI CGCG 5 cut(s) 616, 1806, 2223, 2454, 2756
BstH2I RGCGCY 1 cut(s) 524
BstHHI GCGC 9 cut(s) 523, 1173, 1806, 1808, 2223, 2225, 2454, 2456, 2756
BstKTI GATC 9 cut(s) 1027, 1112, 1462, 1504, 1560, 1659, 1735, 2286, 2626
BstMBI GATC 9 cut(s) 1024, 1109, 1459, 1501, 1557, 1656, 1732, 2283, 2623
BstNI CCWGG 8 cut(s) 120, 535, 1080, 1478, 1748, 1762, 2173, 2737
BstNSI RCATGY 1 cut(s) 1775
BstSCI CCNGG 8 cut(s) 118, 533, 1078, 1476, 1746, 1760, 2171, 2735
BstSFI CTRYAG 6 cut(s) 372, 417, 708, 950, 1650, 1937
BstUI CGCG 5 cut(s) 616, 1806, 2223, 2454, 2756
BstX2I RGATCY 4 cut(s) 1501, 1732, 2283, 2623
BstYI RGATCY 4 cut(s) 1501, 1732, 2283, 2623
BstZ17I GTATAC 1 cut(s) 2658
BsuI GTATCC 1 cut(s) 755
BsuRI GGCC 4 cut(s) 123, 706, 1603, 1680
BtgI CCRYGG 2 cut(s) 2238, 2712
BtsCI GGATG 9 cut(s) 26, 166, 886, 1145, 1358, 1710, 2109, 2254, 2443
BtsI GCAGTG 2 cut(s) 1210, 2154
BtsIMutI CAGTG 3 cut(s) 776, 1210, 2154
Cac8I GCNNGC 8 cut(s) 484, 708, 1039, 1806, 1944, 2079, 2223, 2454
CaiI CAGNNNCTG 1 cut(s) 299
CciI TCATGA 1 cut(s) 1021
CfoI GCGC 9 cut(s) 523, 1173, 1806, 1808, 2223, 2225, 2454, 2456, 2756
Cfr10I RCCGGY 1 cut(s) 1676
Cfr13I GGNCC 4 cut(s) 251, 401, 943, 2272
CseI GACGC 4 cut(s) 605, 622, 1457, 2697
DdeI CTNAG 6 cut(s) 104, 807, 910, 938, 1505, 1554
DpnI GATC 9 cut(s) 1026, 1111, 1461, 1503, 1559, 1658, 1734, 2285, 2625
DpnII GATC 9 cut(s) 1024, 1109, 1459, 1501, 1557, 1656, 1732, 2283, 2623
DraI TTTAAA 1 cut(s) 867
DriI GACNNNNNGTC 2 cut(s) 1068, 1375
EaeI YGGCCR 2 cut(s) 121, 1678
Eam1104I CTCTTC 4 cut(s) 1920, 2058, 2202, 2427
Eam1105I GACNNNNNGTC 2 cut(s) 1068, 1375
EarI CTCTTC 4 cut(s) 1920, 2058, 2202, 2427
EciI GGCGGA 1 cut(s) 1553
Ecl136II GAGCTC 1 cut(s) 902
Eco130I CCWWGG 4 cut(s) 2238, 2469, 2511, 2712
Eco24I GRGCYC 2 cut(s) 904, 1043
Eco31I GGTCTC 1 cut(s) 2726
Eco47I GGWCC 4 cut(s) 251, 401, 943, 2272
Eco47III AGCGCT 1 cut(s) 522
Eco53kI GAGCTC 1 cut(s) 902
Eco57I CTGAAG 3 cut(s) 1626, 2340, 2501
Eco88I CYCGRG 2 cut(s) 503, 1380
EcoICRI GAGCTC 1 cut(s) 902
EcoO109I RGGNCCY 3 cut(s) 401, 943, 2272
EcoRI GAATTC 1 cut(s) 2365
EcoRII CCWGG 8 cut(s) 118, 533, 1078, 1476, 1746, 1760, 2171, 2735
EcoT14I CCWWGG 4 cut(s) 2238, 2469, 2511, 2712
EcoT22I ATGCAT 2 cut(s) 1773, 2572
EcoT38I GRGCYC 2 cut(s) 904, 1043
ErhI CCWWGG 4 cut(s) 2238, 2469, 2511, 2712
Esp3I CGTCTC 2 cut(s) 39, 2667
FalI AAGNNNNNCTT 6 cut(s) 260, 292, 879, 911, 1415, 1447
FaqI GGGAC 6 cut(s) 177, 387, 1253, 1541, 2387, 2611
FauI CCCGC 1 cut(s) 1030
FbaI TGATCA 2 cut(s) 1459, 1656
FblI GTMKAC 2 cut(s) 2657, 2769
FokI GGATG 9 cut(s) 13, 153, 893, 1132, 1345, 1717, 2116, 2261, 2450
FriOI GRGCYC 2 cut(s) 904, 1043
GlaI GCGC 9 cut(s) 522, 1172, 1805, 1807, 2222, 2224, 2453, 2455, 2755
GsuI CTGGAG 2 cut(s) 705, 2155
HaeII RGCGCY 1 cut(s) 524
HaeIII GGCC 4 cut(s) 123, 706, 1603, 1680
HapII CCGG 2 cut(s) 1677, 1715
HgaI GACGC 4 cut(s) 605, 622, 1457, 2697
HhaI GCGC 9 cut(s) 523, 1173, 1806, 1808, 2223, 2225, 2454, 2456, 2756
Hin6I GCGC 9 cut(s) 521, 1171, 1804, 1806, 2221, 2223, 2452, 2454, 2754
HinP1I GCGC 9 cut(s) 521, 1171, 1804, 1806, 2221, 2223, 2452, 2454, 2754
HincII GTYRAC 1 cut(s) 2770
HindII GTYRAC 1 cut(s) 2770
HindIII AAGCTT 3 cut(s) 1815, 1953, 2088
HpaII CCGG 2 cut(s) 1677, 1715
HphI GGTGA 3 cut(s) 703, 1492, 1666
Hpy166II GTNNAC 7 cut(s) 638, 682, 1235, 1316, 1483, 2658, 2770
Hpy188I TCNGA 5 cut(s) 649, 1557, 1597, 2248, 2488
Hpy8I GTNNAC 7 cut(s) 638, 682, 1235, 1316, 1483, 2658, 2770
Hpy99I CGWCG 1 cut(s) 2410
HpyAV CCTTC 6 cut(s) 805, 956, 1441, 1601, 1891, 2032
HpyCH4III ACNGT 8 cut(s) 581, 743, 1137, 1651, 1743, 1878, 2634, 2691
HpyCH4IV ACGT 5 cut(s) 48, 1069, 1114, 2393, 2408
HpyF3I CTNAG 6 cut(s) 104, 807, 910, 938, 1505, 1554
HpySE526I ACGT 5 cut(s) 48, 1069, 1114, 2393, 2408
HspAI GCGC 9 cut(s) 521, 1171, 1804, 1806, 2221, 2223, 2452, 2454, 2754
Ksp22I TGATCA 2 cut(s) 1459, 1656
Kzo9I GATC 9 cut(s) 1024, 1109, 1459, 1501, 1557, 1656, 1732, 2283, 2623
LmnI GCTCC 8 cut(s) 631, 724, 899, 1178, 1479, 1792, 2174, 2654
LweI GCATC 7 cut(s) 205, 250, 298, 310, 520, 706, 2134
MaeII ACGT 5 cut(s) 48, 1069, 1114, 2393, 2408
MaeIII GTNAC 5 cut(s) 1672, 2185, 2314, 2533, 2665
MalI GATC 9 cut(s) 1026, 1111, 1461, 1503, 1559, 1658, 1734, 2285, 2625
MboI GATC 9 cut(s) 1024, 1109, 1459, 1501, 1557, 1656, 1732, 2283, 2623
MflI RGATCY 4 cut(s) 1501, 1732, 2283, 2623
MhlI GDGCHC 2 cut(s) 904, 1043
MlsI TGGCCA 1 cut(s) 123
MluI ACGCGT 1 cut(s) 614
MluNI TGGCCA 1 cut(s) 123
MlyI GAGTC 5 cut(s) 515, 559, 1057, 2252, 2775
MmeI TCCRAC 6 cut(s) 1200, 1617, 2091, 2226, 2352, 2466
Mox20I TGGCCA 1 cut(s) 123
Mph1103I ATGCAT 2 cut(s) 1773, 2572
MroXI GAANNNNTTC 1 cut(s) 2562
MscI TGGCCA 1 cut(s) 123
MslI CAYNNNNRTG 2 cut(s) 288, 1184
Msp20I TGGCCA 1 cut(s) 123
MspI CCGG 2 cut(s) 1677, 1715
MspR9I CCNGG 8 cut(s) 120, 535, 1080, 1478, 1748, 1762, 2173, 2737
MvaI CCWGG 8 cut(s) 120, 535, 1080, 1478, 1748, 1762, 2173, 2737
MvnI CGCG 5 cut(s) 616, 1806, 2223, 2454, 2756
NcoI CCATGG 2 cut(s) 2238, 2712
NdeII GATC 9 cut(s) 1024, 1109, 1459, 1501, 1557, 1656, 1732, 2283, 2623
NlaIV GGNNCC 5 cut(s) 403, 944, 1429, 1475, 2274
NmuCI GTSAC 2 cut(s) 1672, 2665
NsiI ATGCAT 2 cut(s) 1773, 2572
NspI RCATGY 1 cut(s) 1775
NspV TTCGAA 1 cut(s) 76
PacI TTAATTAA 1 cut(s) 969
PaeR7I CTCGAG 2 cut(s) 503, 1380
PagI TCATGA 1 cut(s) 1021
PauI GCGCGC 3 cut(s) 1804, 2221, 2452
PdmI GAANNNNTTC 1 cut(s) 2562
PfeI GAWTC 7 cut(s) 225, 296, 773, 854, 1419, 1640, 2336
PflFI GACNNNGTC 1 cut(s) 1670
PflMI CCANNNNNTGG 2 cut(s) 540, 1079
PfoI TCCNGGA 2 cut(s) 1746, 2171
PleI GAGTC 5 cut(s) 514, 558, 1057, 2251, 2774
PpsI GAGTC 5 cut(s) 514, 558, 1057, 2251, 2774
Ppu21I YACGTR 1 cut(s) 1115
PpuMI RGGWCCY 3 cut(s) 401, 943, 2272
PsiI TTATAA 1 cut(s) 150
Psp124BI GAGCTC 1 cut(s) 904
Psp5II RGGWCCY 3 cut(s) 401, 943, 2272
Psp6I CCWGG 8 cut(s) 118, 533, 1078, 1476, 1746, 1760, 2171, 2735
PspGI CCWGG 8 cut(s) 118, 533, 1078, 1476, 1746, 1760, 2171, 2735
PspN4I GGNNCC 5 cut(s) 403, 944, 1429, 1475, 2274
PspPI GGNCC 4 cut(s) 251, 401, 943, 2272
PspPPI RGGWCCY 3 cut(s) 401, 943, 2272
PspXI VCTCGAGB 1 cut(s) 503
PstI CTGCAG 4 cut(s) 376, 421, 712, 1941
PstNI CAGNNNCTG 1 cut(s) 299
PsuI RGATCY 4 cut(s) 1501, 1732, 2283, 2623
PsyI GACNNNGTC 1 cut(s) 1670
PteI GCGCGC 3 cut(s) 1804, 2221, 2452
RseI CAYNNNNRTG 2 cut(s) 288, 1184
SacI GAGCTC 1 cut(s) 904
SalI GTCGAC 1 cut(s) 2768
Sau3AI GATC 9 cut(s) 1024, 1109, 1459, 1501, 1557, 1656, 1732, 2283, 2623
Sau96I GGNCC 4 cut(s) 251, 401, 943, 2272
ScaI AGTACT 3 cut(s) 755, 1592, 2044
SchI GAGTC 5 cut(s) 515, 559, 1057, 2252, 2775
ScrFI CCNGG 8 cut(s) 120, 535, 1080, 1478, 1748, 1762, 2173, 2737
SduI GDGCHC 2 cut(s) 904, 1043
SfaNI GCATC 7 cut(s) 205, 250, 298, 310, 520, 706, 2134
SfcI CTRYAG 6 cut(s) 372, 417, 708, 950, 1650, 1937
Sfr274I CTCGAG 2 cut(s) 503, 1380
SfuI TTCGAA 1 cut(s) 76
SinI GGWCC 4 cut(s) 251, 401, 943, 2272
SlaI CTCGAG 2 cut(s) 503, 1380
SmiMI CAYNNNNRTG 2 cut(s) 288, 1184
SmlI CTYRAG 5 cut(s) 503, 903, 1028, 1380, 2158
SmoI CTYRAG 5 cut(s) 503, 903, 1028, 1380, 2158
SpeI ACTAGT 1 cut(s) 2600
SsiI CCGC 4 cut(s) 1037, 1538, 1801, 2218
SspI AATATT 1 cut(s) 2293
SstI GAGCTC 1 cut(s) 904
StyD4I CCNGG 8 cut(s) 118, 533, 1078, 1476, 1746, 1760, 2171, 2735
StyI CCWWGG 4 cut(s) 2238, 2469, 2511, 2712
TaaI ACNGT 8 cut(s) 581, 743, 1137, 1651, 1743, 1878, 2634, 2691
TaiI ACGT 5 cut(s) 51, 1072, 1117, 2396, 2411
TaqI TCGA 8 cut(s) 76, 381, 493, 504, 1381, 1434, 1958, 2769
TatI WGTACW 4 cut(s) 753, 1590, 2042, 2120
TauI GCSGC 2 cut(s) 1804, 2221
TfiI GAWTC 7 cut(s) 225, 296, 773, 854, 1419, 1640, 2336
TscAI CASTG 3 cut(s) 783, 1210, 2154
TseFI GTSAC 2 cut(s) 1672, 2665
Tsp45I GTSAC 2 cut(s) 1672, 2665
TspDTI ATGAA 9 cut(s) 590, 1358, 1564, 1683, 1938, 2120, 2555, 2571, 2603
TspGWI ACGGA 1 cut(s) 2007
TspRI CASTG 3 cut(s) 783, 1210, 2154
Tth111I GACNNNGTC 1 cut(s) 1670
Van91I CCANNNNNTGG 2 cut(s) 540, 1079
VpaK11BI GGWCC 4 cut(s) 251, 401, 943, 2272
XapI RAATTY 3 cut(s) 362, 727, 2365
XbaI TCTAGA 1 cut(s) 1643
XceI RCATGY 1 cut(s) 1775
XhoI CTCGAG 2 cut(s) 503, 1380
XmiI GTMKAC 2 cut(s) 2657, 2769
XmnI GAANNNNTTC 1 cut(s) 2562
ZrmI AGTACT 3 cut(s) 755, 1592, 2044
Zsp2I ATGCAT 2 cut(s) 1773, 2572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.