Rw4G034430

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0018429
Physical Location & Seq
Reverse (-)
61507286 .. 61510641
3356 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034430.1

Sequence Viewer

Length: 2652 bp
ATGGCAGCCAAATGTGAAACAAGCTCATCCAAAAAGGCTAATAGAGACGTTCTCCTAGAGATTGAGGCAAAGGTTCGAAAATGTTGGGAGGATAAGAAAGTTCTCAGTTCTAAAAAGCCTGGCCACAAGTTCTTTGAAAAGTTCCCTTATAATTGTTTTATGGGACATCCATTTAGTCTCTCAAAGCTAGACTGGCTGCCAGAGTTTCCTATTGATGCCATGAGATTCGCTTTGGCTTATGTTTGTGATGGTCCTGTTGATGCTGCAAAGATTAACTTTCTACATAGCAATGCAGATTCTGTATTTGATGCTGCATACGATGCCATCATAAGTCTCACTAAAGAGATGTCATGGCACGAAGAATTTTTGGCTGCAGATTTCGAGTATTGTTTGAGAACAGGTCCCCTCTCTACTTCTGCAGATAAAGTATTTGCCAATGACATAAACATTGCCTTGAACAAGACCAAACAGCATTATGAGGCTTGCCAGTTTCGAGAAGCCCTCGAGTCTGGCTTTCTAGCGCTTCAAGATGCCAGGGATTGGTACAAGAGTCTATGCGGCGGTACAAATGCAATGAACCGTGATTTGGTGTGGCGTTTTATGGAAATGCAGACGCGTCTTGTTGCTCCAATCTGTCCACATTATTCGGAATATGTGTGGAGAGAACTTTTGCACAATGTAAACTTTGTGGTGAAAGCAGACTGGCCTGCAGCCGATGCTCCAGAAAATTCAACCCTCCACAGTATCCATAAGTACTTGAAAAGCATAACTGATTCACTGAAAAAACAGATTGAAAGTGAGAGAACTAAGAAGGTATGCCCGAACATAGAAGTTTTAATATATGTCAAGGAGGAATCATTTAAATTAAAGATGAGTGAGGATGGGGAAGTTGAACTTGGAGCTCAAGCCTTAGATTTTAAGCTGCCATTTAGAGAGCTTAGGGTCCTTCTACAGAACTTGGACTTAATTAAGAGGCAAATCGGCGTTGAAAGGGTGCAAGTTCTGTCAGCTACCAACCCTCATGATCTTGAGAAAGCAGGCTCTCATGTTGAACTGATTAAGACTCTACGTCCAATACCTGGAAAGCCAATTAAACATGGTGTACGGGACTTACCAAACCAAGTAGCTAGTAAATGCAGCAATGAGGATATTCAAGTTCTTGAATGGTTTGATAAGCAAAGTAAACAGAGGCATGGTATAGAGGAAAAGATGAACCAATTTTCTACATCCACAAGAGACAACAAGTCTCGAGATGGTTTAAACTCTTATGCTCCATGGAAGTTAAGATTCAAGGGTTCCTTCGACAAGGCAATGGACGCTGCGTGTGATCAAGACAAATGGCTCCTGGTGAACTTGCAATGCAGGGAGGATCTCAGCTCACATAAGCTTAATCGGGACATCTGGGCGGACAAAGACATCTCTCAGATCATTAGCACCAATTTCATCTTTTGGCAGGAGTACTCTGATTGGCCTGAAGGCACTATGGTTTCCAGCAACTACAAGTTGGATTCTAGAACTGTAGTGATCATCATTGACCCCGTCACCGGCCAAGCATTGCGTTCATGGAATGGGATGGTTCAACCGGACGATTTGCTGCTGGATCTATTACCGTTCCTGGATAGTAGCCCCAGGAATATGCATGTAGCCAAAGAGGAGGAGGAGCAACTGCGGCGCGCATTGGAAGCTTCGGTGGAAAGCGTGAAGTTTGAGACTTTTGGAAGAACCTCCAAAGAGTATCTAGTACCGTCCTTCAATACTAGACCCATGCCTAACAAAGCAACTGAAATAGATGAAGAGGAGGAACTGCAGCGTGCACTGGAAGCTTCGATGGAAAGCATGAAGTTTGAGACAAGTAATGCCAGTACCTCAAAAGAGGCTCTAGTTCCGTCCTTCAATACTAGCCCCATTAGTACTAGAGTAAGTGAAGAAGAAGAGGAACGGCTGCACGCATTGGAAGCTTTTGTGGAAAGGATGAAGTTGGAGACAAGTACAGCAAGTACCTCCAAAGGAGATGCAGTGTCGTCCTTGAGTAGTAGCTCCAGGAATACTTATGTAACTAGACCAGAAGAAGAAGAAGAGGAACTGCGGCGCGCATTGGCAGCTTCCATGGAGTCGGATGGTGGAAGTACCTCCAAAGAGGACCCACTAGAGATCTTCAATATTAAACCCATAATTACTTACGTAACTGAAGATGACGAGGAGGAATCTCGGTGTGCAATGGCAGATTTCGTGAATTCGTTGGAGACAAGTAGTGCAAGTACGTCTAAAGTCCCGACGTTTAATAATGCTACTAGAGAAGAAGAGGATGAGGAATTGCAGCGAGCATTGGTGGGTTCCTTGGAAAGCCTGAAGTCGGAGACAAGTAGTGCAAGTACCTCCAAGGTTTCCAAGAGTACTTATGTAACTAAAGAAGAAGAAGAAGAAGAAGATGATGAACTGCTTCATGCATTGGAAGTGGAAAACATGAAGTGGGGGACTAGTGGAAGTACCTCTAAAGAAGATCTAGTACCGTTCTCCAATAGTAGCTCCATGTATACTGATATGACTAGAGACGAAGATGAGGAACTGTGTCGCGCATTAGCAGCGTCCATGGAAAGTTTCAAGGTGGAGACATGGACTAGAGGAAGAAGCTCGAAAGAGAGAGTCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

883

Amino Acids

100.42

Weight (kDa)

4.98

Isoelectric Point (pI)

52.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anticodon_1 PF08264 141 - 265 3.5e-15 Anticodon-binding domain of tRNA ligase
Thioredoxin_7 PF13899 433 - 486 3.9e-10 Thioredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33710
rosa_chinensis RchiOBHm_Chr4g0443181
rosa_laevigata RLG00000005949
rosa_multiflora Rmu_sc0007968.1_g000005
rosa_roxburghii Rroxscaffold_5G00383780
rosa_rugosa Rorug04G0344700
rosa_samantha Rh4BG410900 Rh4CG426400 Rh4DG406000
rosa_wichuraiana Rw4G034430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 150
AccB7I CCANNNNNTGG 2 cut(s) 540, 1079
AccI GTMKAC 2 cut(s) 2534, 2646
AccII CGCG 4 cut(s) 616, 1674, 2091, 2574
AciI CCGC 5 cut(s) 558, 561, 1406, 1669, 2086
AclWI GGATC 2 cut(s) 1377, 1608
AcoI YGGCCR 2 cut(s) 121, 1546
AcsI RAATTY 3 cut(s) 362, 727, 2233
AcuI CTGAAG 3 cut(s) 1494, 2208, 2369
AfeI AGCGCT 1 cut(s) 522
AfiI CCNNNNNNNGG 5 cut(s) 540, 586, 1079, 1544, 2353
AflIII ACRYGT 1 cut(s) 614
AhdI GACNNNNNGTC 2 cut(s) 1068, 1243
AhlI ACTAGT 1 cut(s) 2477
AjnI CCWGG 7 cut(s) 118, 533, 1078, 1344, 1614, 1628, 2039
AjuI GAANNNNNNNTTGG 4 cut(s) 569, 601, 879, 911
Alw21I GWGCWC 2 cut(s) 904, 1816
Alw44I GTGCAC 1 cut(s) 1812
AlwI GGATC 2 cut(s) 1377, 1608
AlwNI CAGNNNCTG 1 cut(s) 299
Ama87I CYCGRG 2 cut(s) 503, 1248
Aor51HI AGCGCT 1 cut(s) 522
AoxI GGCC 4 cut(s) 121, 704, 1469, 1546
ApaLI GTGCAC 1 cut(s) 1812
ApoI RAATTY 3 cut(s) 362, 727, 2233
Asp700I GAANNNNTTC 1 cut(s) 2439
AspLEI GCGC 6 cut(s) 523, 1674, 1676, 2091, 2093, 2576
AspS9I GGNCC 4 cut(s) 251, 401, 943, 2140
AsuHPI GGTGA 3 cut(s) 703, 1360, 1534
AsuII TTCGAA 1 cut(s) 76
AvaI CYCGRG 2 cut(s) 503, 1248
AvaII GGWCC 4 cut(s) 251, 401, 943, 2140
BaeGI GKGCMC 1 cut(s) 1816
BaeI ACNNNNGTAYC 2 cut(s) 535, 568
BalI TGGCCA 1 cut(s) 123
BanII GRGCYC 1 cut(s) 904
BarI GAAGNNNNNNTAC 2 cut(s) 2490, 2522
Bbv12I GWGCWC 2 cut(s) 904, 1816
BccI CCATC 7 cut(s) 242, 332, 875, 1247, 1567, 1822, 2111
BceAI ACGGC 1 cut(s) 1955
BciT130I CCWGG 7 cut(s) 120, 535, 1080, 1346, 1616, 1630, 2041
BciVI GTATCC 1 cut(s) 755
BclI TGATCA 2 cut(s) 1327, 1524
BcuI ACTAGT 1 cut(s) 2477
BfmI CTRYAG 6 cut(s) 372, 417, 708, 950, 1518, 1805
BfoI RGCGCY 1 cut(s) 524
BfuI GTATCC 1 cut(s) 755
BglII AGATCT 2 cut(s) 2151, 2500
BmcAI AGTACT 4 cut(s) 755, 1460, 1912, 2395
Bme1390I CCNGG 7 cut(s) 120, 535, 1080, 1346, 1616, 1630, 2041
Bme18I GGWCC 4 cut(s) 251, 401, 943, 2140
BmeRI GACNNNNNGTC 2 cut(s) 1068, 1243
BmeT110I CYCGRG 2 cut(s) 503, 1248
BmgT120I GGNCC 4 cut(s) 251, 401, 943, 2140
BmiI GGNNCC 6 cut(s) 403, 944, 1297, 1343, 2142, 2335
BmrFI CCNGG 7 cut(s) 120, 535, 1080, 1346, 1616, 1630, 2041
BmsI GCATC 7 cut(s) 205, 250, 298, 310, 520, 706, 2002
BplI GAGNNNNNCTC 4 cut(s) 36, 68, 486, 518
BpmI CTGGAG 2 cut(s) 705, 2023
Bpu10I CCTNAGC 1 cut(s) 938
Bpu14I TTCGAA 1 cut(s) 76
BpuEI CTTGAG 3 cut(s) 888, 1049, 2047
BsaAI YACGTR 1 cut(s) 2182
BsaJI CCNNGG 7 cut(s) 534, 1274, 1628, 2106, 2337, 2379, 2589
BsaWI WCCGGW 1 cut(s) 1582
BsaXI ACNNNNNCTCC 2 cut(s) 2001, 2031
Bsc4I CCNNNNNNNGG 5 cut(s) 540, 586, 1079, 1544, 2353
Bse118I RCCGGY 1 cut(s) 1544
Bse1I ACTGG 5 cut(s) 197, 487, 707, 1821, 1860
Bse3DI GCAATG 8 cut(s) 295, 447, 579, 1147, 1317, 1364, 1553, 2223
BseBI CCWGG 7 cut(s) 120, 535, 1080, 1346, 1616, 1630, 2041
BseDI CCNNGG 7 cut(s) 534, 1274, 1628, 2106, 2337, 2379, 2589
BseGI GGATG 8 cut(s) 26, 166, 886, 1226, 1578, 1977, 2122, 2311
BseLI CCNNNNNNNGG 5 cut(s) 540, 586, 1079, 1544, 2353
BseMI GCAATG 8 cut(s) 295, 447, 579, 1147, 1317, 1364, 1553, 2223
BseMII CTCAG 3 cut(s) 118, 1387, 1436
BseNI ACTGG 5 cut(s) 197, 487, 707, 1821, 1860
BsePI GCGCGC 2 cut(s) 1672, 2089
BseRI GAGGAG 5 cut(s) 1667, 1670, 1673, 1811, 2213
BseSI GKGCMC 1 cut(s) 1816
BsgI GTGCAG 1 cut(s) 1928
Bsh1236I CGCG 4 cut(s) 616, 1674, 2091, 2574
BshFI GGCC 4 cut(s) 123, 706, 1471, 1548
BsiHKAI GWGCWC 2 cut(s) 904, 1816
BsiHKCI CYCGRG 2 cut(s) 503, 1248
BsiSI CCGG 2 cut(s) 1545, 1583
BslFI GGGAC 6 cut(s) 177, 387, 1121, 1409, 2255, 2488
BslI CCNNNNNNNGG 5 cut(s) 540, 586, 1079, 1544, 2353
BsmBI CGTCTC 2 cut(s) 39, 2544
BsmFI GGGAC 6 cut(s) 177, 387, 1121, 1409, 2255, 2488
BsnI GGCC 4 cut(s) 123, 706, 1471, 1548
BsoBI CYCGRG 2 cut(s) 503, 1248
Bsp119I TTCGAA 1 cut(s) 76
Bsp1286I GDGCHC 2 cut(s) 904, 1816
Bsp143I GATC 8 cut(s) 1024, 1327, 1369, 1425, 1524, 1600, 2151, 2500
Bsp19I CCATGG 3 cut(s) 1274, 2106, 2589
BspACI CCGC 5 cut(s) 558, 561, 1406, 1669, 2086
BspANI GGCC 4 cut(s) 123, 706, 1471, 1548
BspCNI CTCAG 3 cut(s) 117, 1386, 1435
BspFNI CGCG 4 cut(s) 616, 1674, 2091, 2574
BspHI TCATGA 1 cut(s) 1021
BspLI GGNNCC 6 cut(s) 403, 944, 1297, 1343, 2142, 2335
BspMAI CTGCAG 4 cut(s) 376, 421, 712, 1809
BspPI GGATC 2 cut(s) 1377, 1608
BspT104I TTCGAA 1 cut(s) 76
BsrDI GCAATG 8 cut(s) 295, 447, 579, 1147, 1317, 1364, 1553, 2223
BsrFI RCCGGY 1 cut(s) 1544
BsrI ACTGG 5 cut(s) 197, 487, 707, 1821, 1860
BssAI RCCGGY 1 cut(s) 1544
BssECI CCNNGG 7 cut(s) 534, 1274, 1628, 2106, 2337, 2379, 2589
BssHII GCGCGC 2 cut(s) 1672, 2089
BssMI GATC 8 cut(s) 1024, 1327, 1369, 1425, 1524, 1600, 2151, 2500
BssNAI GTATAC 1 cut(s) 2535
BssT1I CCWWGG 5 cut(s) 1274, 2106, 2337, 2379, 2589
Bst1107I GTATAC 1 cut(s) 2535
Bst2UI CCWGG 7 cut(s) 120, 535, 1080, 1346, 1616, 1630, 2041
Bst4CI ACNGT 7 cut(s) 581, 743, 1519, 1611, 1746, 2511, 2568
Bst6I CTCTTC 4 cut(s) 1788, 1926, 2070, 2295
BstAPI GCANNNNNTGC 2 cut(s) 320, 716
BstBAI YACGTR 1 cut(s) 2182
BstBI TTCGAA 1 cut(s) 76
BstC8I GCNNGC 8 cut(s) 484, 708, 1039, 1674, 1812, 1947, 2091, 2322
BstDEI CTNAG 6 cut(s) 104, 807, 910, 938, 1373, 1422
BstDSI CCRYGG 3 cut(s) 1274, 2106, 2589
BstF5I GGATG 8 cut(s) 26, 166, 886, 1226, 1578, 1977, 2122, 2311
BstFNI CGCG 4 cut(s) 616, 1674, 2091, 2574
BstH2I RGCGCY 1 cut(s) 524
BstHHI GCGC 6 cut(s) 523, 1674, 1676, 2091, 2093, 2576
BstKTI GATC 8 cut(s) 1027, 1330, 1372, 1428, 1527, 1603, 2154, 2503
BstMBI GATC 8 cut(s) 1024, 1327, 1369, 1425, 1524, 1600, 2151, 2500
BstNI CCWGG 7 cut(s) 120, 535, 1080, 1346, 1616, 1630, 2041
BstNSI RCATGY 1 cut(s) 1643
BstSCI CCNGG 7 cut(s) 118, 533, 1078, 1344, 1614, 1628, 2039
BstSFI CTRYAG 6 cut(s) 372, 417, 708, 950, 1518, 1805
BstSLI GKGCMC 1 cut(s) 1816
BstSNI TACGTA 1 cut(s) 2182
BstUI CGCG 4 cut(s) 616, 1674, 2091, 2574
BstX2I RGATCY 4 cut(s) 1369, 1600, 2151, 2500
BstYI RGATCY 4 cut(s) 1369, 1600, 2151, 2500
BstZ17I GTATAC 1 cut(s) 2535
BsuI GTATCC 1 cut(s) 755
BsuRI GGCC 4 cut(s) 123, 706, 1471, 1548
BtgI CCRYGG 3 cut(s) 1274, 2106, 2589
BtsCI GGATG 8 cut(s) 26, 166, 886, 1226, 1578, 1977, 2122, 2311
BtsI GCAGTG 1 cut(s) 2022
BtsIMutI CAGTG 3 cut(s) 776, 1814, 2022
Cac8I GCNNGC 8 cut(s) 484, 708, 1039, 1674, 1812, 1947, 2091, 2322
CaiI CAGNNNCTG 1 cut(s) 299
CciI TCATGA 1 cut(s) 1021
CfoI GCGC 6 cut(s) 523, 1674, 1676, 2091, 2093, 2576
Cfr10I RCCGGY 1 cut(s) 1544
Cfr13I GGNCC 4 cut(s) 251, 401, 943, 2140
CseI GACGC 4 cut(s) 605, 622, 1325, 2574
DdeI CTNAG 6 cut(s) 104, 807, 910, 938, 1373, 1422
DpnI GATC 8 cut(s) 1026, 1329, 1371, 1427, 1526, 1602, 2153, 2502
DpnII GATC 8 cut(s) 1024, 1327, 1369, 1425, 1524, 1600, 2151, 2500
DraI TTTAAA 2 cut(s) 862, 1260
DriI GACNNNNNGTC 2 cut(s) 1068, 1243
EaeI YGGCCR 2 cut(s) 121, 1546
Eam1104I CTCTTC 4 cut(s) 1788, 1926, 2070, 2295
Eam1105I GACNNNNNGTC 2 cut(s) 1068, 1243
EarI CTCTTC 4 cut(s) 1788, 1926, 2070, 2295
EciI GGCGGA 1 cut(s) 1421
Ecl136II GAGCTC 1 cut(s) 902
Eco105I TACGTA 1 cut(s) 2182
Eco130I CCWWGG 5 cut(s) 1274, 2106, 2337, 2379, 2589
Eco24I GRGCYC 1 cut(s) 904
Eco47I GGWCC 4 cut(s) 251, 401, 943, 2140
Eco47III AGCGCT 1 cut(s) 522
Eco53kI GAGCTC 1 cut(s) 902
Eco57I CTGAAG 3 cut(s) 1494, 2208, 2369
Eco88I CYCGRG 2 cut(s) 503, 1248
EcoICRI GAGCTC 1 cut(s) 902
EcoO109I RGGNCCY 3 cut(s) 401, 943, 2140
EcoRI GAATTC 1 cut(s) 2233
EcoRII CCWGG 7 cut(s) 118, 533, 1078, 1344, 1614, 1628, 2039
EcoT14I CCWWGG 5 cut(s) 1274, 2106, 2337, 2379, 2589
EcoT22I ATGCAT 2 cut(s) 1641, 2449
EcoT38I GRGCYC 1 cut(s) 904
ErhI CCWWGG 5 cut(s) 1274, 2106, 2337, 2379, 2589
Esp3I CGTCTC 2 cut(s) 39, 2544
FalI AAGNNNNNCTT 6 cut(s) 260, 292, 879, 911, 1283, 1315
FaqI GGGAC 6 cut(s) 177, 387, 1121, 1409, 2255, 2488
FbaI TGATCA 2 cut(s) 1327, 1524
FblI GTMKAC 2 cut(s) 2534, 2646
FokI GGATG 8 cut(s) 13, 153, 893, 1213, 1585, 1984, 2129, 2318
FriOI GRGCYC 1 cut(s) 904
GlaI GCGC 6 cut(s) 522, 1673, 1675, 2090, 2092, 2575
GsuI CTGGAG 2 cut(s) 705, 2023
HaeII RGCGCY 1 cut(s) 524
HaeIII GGCC 4 cut(s) 123, 706, 1471, 1548
HapII CCGG 2 cut(s) 1545, 1583
HgaI GACGC 4 cut(s) 605, 622, 1325, 2574
HhaI GCGC 6 cut(s) 523, 1674, 1676, 2091, 2093, 2576
Hin6I GCGC 6 cut(s) 521, 1672, 1674, 2089, 2091, 2574
HinP1I GCGC 6 cut(s) 521, 1672, 1674, 2089, 2091, 2574
HincII GTYRAC 1 cut(s) 2647
HindII GTYRAC 1 cut(s) 2647
HindIII AAGCTT 4 cut(s) 1385, 1683, 1821, 1956
HpaII CCGG 2 cut(s) 1545, 1583
HphI GGTGA 3 cut(s) 703, 1360, 1534
Hpy166II GTNNAC 8 cut(s) 638, 682, 1103, 1184, 1351, 1814, 2535, 2647
Hpy188I TCNGA 5 cut(s) 649, 1425, 1465, 2116, 2356
Hpy8I GTNNAC 8 cut(s) 638, 682, 1103, 1184, 1351, 1814, 2535, 2647
Hpy99I CGWCG 1 cut(s) 2278
HpyAV CCTTC 6 cut(s) 805, 956, 1309, 1469, 1759, 1900
HpyCH4III ACNGT 7 cut(s) 581, 743, 1519, 1611, 1746, 2511, 2568
HpyCH4IV ACGT 5 cut(s) 48, 1069, 2181, 2261, 2276
HpyF3I CTNAG 6 cut(s) 104, 807, 910, 938, 1373, 1422
HpySE526I ACGT 5 cut(s) 48, 1069, 2181, 2261, 2276
HspAI GCGC 6 cut(s) 521, 1672, 1674, 2089, 2091, 2574
Ksp22I TGATCA 2 cut(s) 1327, 1524
Kzo9I GATC 8 cut(s) 1024, 1327, 1369, 1425, 1524, 1600, 2151, 2500
LmnI GCTCC 8 cut(s) 631, 724, 899, 1276, 1347, 1660, 2042, 2531
LweI GCATC 7 cut(s) 205, 250, 298, 310, 520, 706, 2002
MaeII ACGT 5 cut(s) 48, 1069, 2181, 2261, 2276
MaeIII GTNAC 4 cut(s) 1540, 2053, 2182, 2401
MalI GATC 8 cut(s) 1026, 1329, 1371, 1427, 1526, 1602, 2153, 2502
MboI GATC 8 cut(s) 1024, 1327, 1369, 1425, 1524, 1600, 2151, 2500
MflI RGATCY 4 cut(s) 1369, 1600, 2151, 2500
MhlI GDGCHC 2 cut(s) 904, 1816
MlsI TGGCCA 1 cut(s) 123
MluI ACGCGT 1 cut(s) 614
MluNI TGGCCA 1 cut(s) 123
MlyI GAGTC 5 cut(s) 515, 559, 1057, 2120, 2652
MmeI TCCRAC 5 cut(s) 1485, 1959, 2094, 2220, 2334
Mox20I TGGCCA 1 cut(s) 123
Mph1103I ATGCAT 2 cut(s) 1641, 2449
MroXI GAANNNNTTC 1 cut(s) 2439
MscI TGGCCA 1 cut(s) 123
MslI CAYNNNNRTG 1 cut(s) 288
Msp20I TGGCCA 1 cut(s) 123
MspI CCGG 2 cut(s) 1545, 1583
MspR9I CCNGG 7 cut(s) 120, 535, 1080, 1346, 1616, 1630, 2041
MssI GTTTAAAC 1 cut(s) 1260
MvaI CCWGG 7 cut(s) 120, 535, 1080, 1346, 1616, 1630, 2041
MvnI CGCG 4 cut(s) 616, 1674, 2091, 2574
NcoI CCATGG 3 cut(s) 1274, 2106, 2589
NdeII GATC 8 cut(s) 1024, 1327, 1369, 1425, 1524, 1600, 2151, 2500
NlaIV GGNNCC 6 cut(s) 403, 944, 1297, 1343, 2142, 2335
NmuCI GTSAC 1 cut(s) 1540
NsiI ATGCAT 2 cut(s) 1641, 2449
NspI RCATGY 1 cut(s) 1643
NspV TTCGAA 1 cut(s) 76
PacI TTAATTAA 1 cut(s) 969
PaeR7I CTCGAG 2 cut(s) 503, 1248
PagI TCATGA 1 cut(s) 1021
PauI GCGCGC 2 cut(s) 1672, 2089
PdmI GAANNNNTTC 1 cut(s) 2439
PfeI GAWTC 7 cut(s) 225, 296, 773, 854, 1287, 1508, 2204
PflFI GACNNNGTC 1 cut(s) 1538
PflMI CCANNNNNTGG 2 cut(s) 540, 1079
PfoI TCCNGGA 2 cut(s) 1614, 2039
PleI GAGTC 5 cut(s) 514, 558, 1057, 2119, 2651
PmeI GTTTAAAC 1 cut(s) 1260
PpsI GAGTC 5 cut(s) 514, 558, 1057, 2119, 2651
Ppu21I YACGTR 1 cut(s) 2182
PpuMI RGGWCCY 3 cut(s) 401, 943, 2140
PsiI TTATAA 1 cut(s) 150
Psp124BI GAGCTC 1 cut(s) 904
Psp5II RGGWCCY 3 cut(s) 401, 943, 2140
Psp6I CCWGG 7 cut(s) 118, 533, 1078, 1344, 1614, 1628, 2039
PspGI CCWGG 7 cut(s) 118, 533, 1078, 1344, 1614, 1628, 2039
PspN4I GGNNCC 6 cut(s) 403, 944, 1297, 1343, 2142, 2335
PspPI GGNCC 4 cut(s) 251, 401, 943, 2140
PspPPI RGGWCCY 3 cut(s) 401, 943, 2140
PspXI VCTCGAGB 1 cut(s) 503
PstI CTGCAG 4 cut(s) 376, 421, 712, 1809
PstNI CAGNNNCTG 1 cut(s) 299
PsuI RGATCY 4 cut(s) 1369, 1600, 2151, 2500
PsyI GACNNNGTC 1 cut(s) 1538
PteI GCGCGC 2 cut(s) 1672, 2089
RseI CAYNNNNRTG 1 cut(s) 288
SacI GAGCTC 1 cut(s) 904
SalI GTCGAC 1 cut(s) 2645
Sau3AI GATC 8 cut(s) 1024, 1327, 1369, 1425, 1524, 1600, 2151, 2500
Sau96I GGNCC 4 cut(s) 251, 401, 943, 2140
ScaI AGTACT 4 cut(s) 755, 1460, 1912, 2395
SchI GAGTC 5 cut(s) 515, 559, 1057, 2120, 2652
ScrFI CCNGG 7 cut(s) 120, 535, 1080, 1346, 1616, 1630, 2041
SduI GDGCHC 2 cut(s) 904, 1816
SfaNI GCATC 7 cut(s) 205, 250, 298, 310, 520, 706, 2002
SfcI CTRYAG 6 cut(s) 372, 417, 708, 950, 1518, 1805
Sfr274I CTCGAG 2 cut(s) 503, 1248
SfuI TTCGAA 1 cut(s) 76
SinI GGWCC 4 cut(s) 251, 401, 943, 2140
SlaI CTCGAG 2 cut(s) 503, 1248
SmiI ATTTAAAT 1 cut(s) 862
SmiMI CAYNNNNRTG 1 cut(s) 288
SmlI CTYRAG 5 cut(s) 503, 903, 1028, 1248, 2026
SmoI CTYRAG 5 cut(s) 503, 903, 1028, 1248, 2026
SnaBI TACGTA 1 cut(s) 2182
SpeI ACTAGT 1 cut(s) 2477
SsiI CCGC 5 cut(s) 558, 561, 1406, 1669, 2086
SspI AATATT 1 cut(s) 2161
SstI GAGCTC 1 cut(s) 904
StyD4I CCNGG 7 cut(s) 118, 533, 1078, 1344, 1614, 1628, 2039
StyI CCWWGG 5 cut(s) 1274, 2106, 2337, 2379, 2589
SwaI ATTTAAAT 1 cut(s) 862
TaaI ACNGT 7 cut(s) 581, 743, 1519, 1611, 1746, 2511, 2568
TaiI ACGT 5 cut(s) 51, 1072, 2184, 2264, 2279
TaqI TCGA 9 cut(s) 76, 381, 493, 504, 1249, 1302, 1826, 2633, 2646
TatI WGTACW 5 cut(s) 753, 1458, 1910, 1988, 2393
TauI GCSGC 3 cut(s) 561, 1672, 2089
TfiI GAWTC 7 cut(s) 225, 296, 773, 854, 1287, 1508, 2204
TscAI CASTG 3 cut(s) 783, 1821, 2022
TseFI GTSAC 1 cut(s) 1540
Tsp45I GTSAC 1 cut(s) 1540
TspGWI ACGGA 1 cut(s) 1875
TspRI CASTG 3 cut(s) 783, 1821, 2022
Tth111I GACNNNGTC 1 cut(s) 1538
Van91I CCANNNNNTGG 2 cut(s) 540, 1079
VneI GTGCAC 1 cut(s) 1812
VpaK11BI GGWCC 4 cut(s) 251, 401, 943, 2140
XapI RAATTY 3 cut(s) 362, 727, 2233
XbaI TCTAGA 1 cut(s) 1511
XceI RCATGY 1 cut(s) 1643
XhoI CTCGAG 2 cut(s) 503, 1248
XmiI GTMKAC 2 cut(s) 2534, 2646
XmnI GAANNNNTTC 1 cut(s) 2439
ZrmI AGTACT 4 cut(s) 755, 1460, 1912, 2395
Zsp2I ATGCAT 2 cut(s) 1641, 2449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.