RLG00000006348

Phloem protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0020000
Physical Location & Seq
Forward (+)
6455949 .. 6456762
814 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006348

Sequence Viewer

Length: 618 bp
ATGGAGAACGCAGCGGTGATGATGGACTTGCAGGAATTGCCAGAAGGCTGCATAGCCAACGTGATGTCGCTGATGACTCCTCAAGACGCGTGCAGACTATCGTTGATTTTGAGGAATTTCAAGTCAGCGGCGGACTCCGACGCCGTTTGGGTTAAGTTCCTTCCACATGAGACCAATGAGATCCTATCCCACTCCGAATCTGCAATCCACGCCAAATCCAAGAAGGAGCTTTACTTCACTCTCTCTCACAACCCCATCCTCATCGACGATAGCAAGATGGGAATTGTGATTTGTAAATTCATATTGGAATTTTTTGTTGGTATTGATTTGATTCGAAGATGTTGCTCTACGAGCAGAAGAAGAGTCCCAAGGGCAACAGGGTTCTTGATCATCATTACTGTGTTGGGCAGTGCCGGCCCGATCCAATTTGTCATCAATGAGGAGATGCTTGTCGCTGCGGTCATCGATACCGCCTTGAAATCCTATGCTCCTAAAGGTCGCCTTCCGATTCTTGGCTCTGATCTCAATGAGTTCATGTTCTACTGGCCCATTGCTGGACCTGATGGTAATATCTCCATAGTCTCTTTTATCACTTTTGAATCTCTTCCTGAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

206

Amino Acids

22.8

Weight (kDa)

5.38

Isoelectric Point (pI)

47.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 127 - 182 2.1e-14 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020000)

Species Orthologous Gene IDs
rosa_laevigata RLG00000006348 RLG00000009287 RLG00000011184
rosa_multiflora Rmu_sc0000058.1_g000008 Rmu_sc0003382.1_g000029 Rmu_sc0004790.1_g000002
rosa_samantha Rh7AG377000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 89
AciI CCGC 5 cut(s) 14, 128, 131, 458, 471
AclWI GGATC 2 cut(s) 175, 415
AcsI RAATTY 4 cut(s) 115, 296, 308, 611
AcyI GRCGYC 1 cut(s) 141
AfiI CCNNNNNNNGG 2 cut(s) 512, 554
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 3 cut(s) 121, 478, 599
AjuI GAANNNNNNNTTGG 2 cut(s) 300, 332
AluBI AGCT 1 cut(s) 229
AluI AGCT 1 cut(s) 229
Alw26I GTCTC 2 cut(s) 164, 586
AlwI GGATC 2 cut(s) 175, 415
AoxI GGCC 2 cut(s) 415, 545
ApeKI GCWGC 3 cut(s) 11, 48, 455
ApoI RAATTY 4 cut(s) 115, 296, 308, 611
Asp700I GAANNNNTTC 1 cut(s) 603
AspS9I GGNCC 3 cut(s) 416, 546, 557
AsuHPI GGTGA 1 cut(s) 28
AsuII TTCGAA 1 cut(s) 334
AvaII GGWCC 1 cut(s) 557
BarI GAAGNNNNNNTAC 2 cut(s) 215, 247
BbvI GCAGC 3 cut(s) 23, 35, 442
BccI CCATC 4 cut(s) 16, 263, 271, 557
BceAI ACGGC 1 cut(s) 128
BcgI CGANNNNNNTGC 2 cut(s) 324, 358
BclI TGATCA 1 cut(s) 387
BcoDI GTCTC 2 cut(s) 164, 586
BisI GCNGC 4 cut(s) 12, 49, 129, 456
BlsI GCNGC 4 cut(s) 13, 50, 130, 457
Bme18I GGWCC 1 cut(s) 557
BmgT120I GGNCC 3 cut(s) 416, 546, 557
BmsI GCATC 1 cut(s) 435
Bpu14I TTCGAA 1 cut(s) 334
BpuEI CTTGAG 1 cut(s) 66
Bsa29I ATCGAT 1 cut(s) 465
BsaHI GRCGYC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 368
Bsc4I CCNNNNNNNGG 2 cut(s) 512, 554
Bse118I RCCGGY 1 cut(s) 413
Bse1I ACTGG 1 cut(s) 548
Bse3DI GCAATG 1 cut(s) 549
BseCI ATCGAT 1 cut(s) 465
BseDI CCNNGG 1 cut(s) 368
BseGI GGATG 1 cut(s) 255
BseLI CCNNNNNNNGG 2 cut(s) 512, 554
BseMI GCAATG 1 cut(s) 549
BseNI ACTGG 1 cut(s) 548
BseRI GAGGAG 2 cut(s) 69, 455
BseXI GCAGC 3 cut(s) 23, 35, 442
BsgI GTGCAG 1 cut(s) 112
Bsh1236I CGCG 1 cut(s) 89
BshFI GGCC 2 cut(s) 417, 547
BshVI ATCGAT 1 cut(s) 465
BsiSI CCGG 1 cut(s) 414
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 2 cut(s) 512, 554
BsmAI GTCTC 2 cut(s) 164, 586
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 2 cut(s) 417, 547
Bso31I GGTCTC 1 cut(s) 164
Bsp119I TTCGAA 1 cut(s) 334
Bsp143I GATC 4 cut(s) 180, 387, 420, 520
BspACI CCGC 5 cut(s) 14, 128, 131, 458, 471
BspANI GGCC 2 cut(s) 417, 547
BspDI ATCGAT 1 cut(s) 465
BspFNI CGCG 1 cut(s) 89
BspPI GGATC 2 cut(s) 175, 415
BspT104I TTCGAA 1 cut(s) 334
BspTNI GGTCTC 1 cut(s) 164
BsrDI GCAATG 1 cut(s) 549
BsrFI RCCGGY 1 cut(s) 413
BsrI ACTGG 1 cut(s) 548
BssAI RCCGGY 1 cut(s) 413
BssECI CCNNGG 1 cut(s) 368
BssMI GATC 4 cut(s) 180, 387, 420, 520
BssNI GRCGYC 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 368
Bst4CI ACNGT 1 cut(s) 400
Bst6I CTCTTC 2 cut(s) 355, 609
BstACI GRCGYC 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 37
BstBI TTCGAA 1 cut(s) 334
BstC8I GCNNGC 2 cut(s) 91, 415
BstF5I GGATG 1 cut(s) 255
BstFNI CGCG 1 cut(s) 89
BstKTI GATC 4 cut(s) 183, 390, 423, 523
BstMAI GTCTC 2 cut(s) 164, 586
BstMBI GATC 4 cut(s) 180, 387, 420, 520
BstMWI GCNNNNNNNGC 4 cut(s) 37, 209, 351, 414
BstUI CGCG 1 cut(s) 89
BstV1I GCAGC 3 cut(s) 23, 35, 442
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
Bsu15I ATCGAT 1 cut(s) 465
BsuRI GGCC 2 cut(s) 417, 547
BsuTUI ATCGAT 1 cut(s) 465
BtsCI GGATG 1 cut(s) 255
BtsI GCAGTG 1 cut(s) 415
BtsIMutI CAGTG 1 cut(s) 415
Cac8I GCNNGC 2 cut(s) 91, 415
Cfr10I RCCGGY 1 cut(s) 413
Cfr13I GGNCC 3 cut(s) 416, 546, 557
ClaI ATCGAT 1 cut(s) 465
CseI GACGC 2 cut(s) 95, 149
CviAII CATG 2 cut(s) 167, 535
CviJI RGCY 6 cut(s) 48, 56, 229, 417, 516, 547
CviKI_1 RGCY 6 cut(s) 48, 56, 229, 417, 516, 547
DpnI GATC 4 cut(s) 182, 389, 422, 522
DpnII GATC 4 cut(s) 180, 387, 420, 520
Eam1104I CTCTTC 2 cut(s) 355, 609
EarI CTCTTC 2 cut(s) 355, 609
EciI GGCGGA 1 cut(s) 146
Eco130I CCWWGG 1 cut(s) 368
Eco31I GGTCTC 1 cut(s) 164
Eco47I GGWCC 1 cut(s) 557
EcoT14I CCWWGG 1 cut(s) 368
ErhI CCWWGG 1 cut(s) 368
FaeI CATG 2 cut(s) 170, 538
FaiI YATR 6 cut(s) 53, 168, 302, 486, 536, 578
FalI AAGNNNNNCTT 2 cut(s) 486, 518
FaqI GGGAC 1 cut(s) 350
FatI CATG 2 cut(s) 166, 534
FbaI TGATCA 1 cut(s) 387
Fnu4HI GCNGC 4 cut(s) 12, 49, 129, 456
FokI GGATG 1 cut(s) 242
Fsp4HI GCNGC 4 cut(s) 12, 49, 129, 456
GluI GCNGC 4 cut(s) 12, 49, 129, 456
HaeIII GGCC 2 cut(s) 417, 547
HapII CCGG 1 cut(s) 414
HgaI GACGC 2 cut(s) 95, 149
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 2 cut(s) 170, 538
HinfI GANTC 7 cut(s) 76, 134, 197, 331, 363, 508, 599
HpaII CCGG 1 cut(s) 414
HphI GGTGA 1 cut(s) 28
Hpy188I TCNGA 4 cut(s) 139, 196, 507, 520
Hpy188III TCNNGA 3 cut(s) 83, 385, 608
Hpy99I CGWCG 2 cut(s) 143, 269
HpyAV CCTTC 4 cut(s) 38, 170, 217, 512
HpyCH4III ACNGT 1 cut(s) 400
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 4 cut(s) 31, 51, 93, 203
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 209, 351, 414
HpySE526I ACGT 1 cut(s) 60
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 2 cut(s) 170, 538
KroI GCCGGC 1 cut(s) 413
KroNI GCCGGC 1 cut(s) 415
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 4 cut(s) 180, 387, 420, 520
LmnI GCTCC 2 cut(s) 226, 493
LpnPI CCDG 7 cut(s) 17, 54, 363, 427, 529, 540, 573
Lsp1109I GCAGC 3 cut(s) 23, 35, 442
LweI GCATC 1 cut(s) 435
MaeII ACGT 1 cut(s) 60
MalI GATC 4 cut(s) 182, 389, 422, 522
MboI GATC 4 cut(s) 180, 387, 420, 520
MboII GAAGA 4 cut(s) 348, 369, 372, 596
MflI RGATCY 1 cut(s) 180
MluCI AATT 7 cut(s) 35, 115, 282, 296, 308, 425, 611
MluI ACGCGT 1 cut(s) 87
MlyI GAGTC 3 cut(s) 70, 128, 372
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 4 cut(s) 90, 105, 269, 433
MroNI GCCGGC 1 cut(s) 413
MroXI GAANNNNTTC 1 cut(s) 603
MseI TTAA 2 cut(s) 153, 616
MslI CAYNNNNRTG 1 cut(s) 398
MspA1I CMGCKG 2 cut(s) 14, 128
MspI CCGG 1 cut(s) 414
MvnI CGCG 1 cut(s) 89
MwoI GCNNNNNNNGC 4 cut(s) 37, 209, 351, 414
NaeI GCCGGC 1 cut(s) 415
NdeII GATC 4 cut(s) 180, 387, 420, 520
NgoMIV GCCGGC 1 cut(s) 413
NlaIII CATG 2 cut(s) 170, 538
NspV TTCGAA 1 cut(s) 334
PdiI GCCGGC 1 cut(s) 415
PdmI GAANNNNTTC 1 cut(s) 603
PfeI GAWTC 4 cut(s) 197, 331, 508, 599
PkrI GCNGC 4 cut(s) 13, 50, 130, 457
PleI GAGTC 3 cut(s) 70, 128, 371
PpsI GAGTC 3 cut(s) 70, 128, 371
PspPI GGNCC 3 cut(s) 416, 546, 557
PsuI RGATCY 1 cut(s) 180
RseI CAYNNNNRTG 1 cut(s) 398
SaqAI TTAA 2 cut(s) 153, 616
SatI GCNGC 4 cut(s) 12, 49, 129, 456
Sau3AI GATC 4 cut(s) 180, 387, 420, 520
Sau96I GGNCC 3 cut(s) 416, 546, 557
SchI GAGTC 3 cut(s) 70, 128, 372
SetI ASST 4 cut(s) 63, 231, 499, 562
SfaNI GCATC 1 cut(s) 435
SfuI TTCGAA 1 cut(s) 334
SinI GGWCC 1 cut(s) 557
SmiMI CAYNNNNRTG 1 cut(s) 398
SmlI CTYRAG 1 cut(s) 81
SmoI CTYRAG 1 cut(s) 81
Sse9I AATT 7 cut(s) 35, 115, 282, 296, 308, 425, 611
SsiI CCGC 5 cut(s) 14, 128, 131, 458, 471
StyI CCWWGG 1 cut(s) 368
TaaI ACNGT 1 cut(s) 400
TaiI ACGT 1 cut(s) 63
TaqI TCGA 3 cut(s) 264, 334, 465
TasI AATT 7 cut(s) 35, 115, 282, 296, 308, 425, 611
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 4 cut(s) 197, 331, 508, 599
Tru1I TTAA 2 cut(s) 153, 616
Tru9I TTAA 2 cut(s) 153, 616
TscAI CASTG 1 cut(s) 415
TseI GCWGC 3 cut(s) 11, 48, 455
TspDTI ATGAA 2 cut(s) 289, 523
TspRI CASTG 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 557
XapI RAATTY 4 cut(s) 115, 296, 308, 611
XmnI GAANNNNTTC 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.