Rh7AG377000

Phloem protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0020000
Physical Location & Seq
Reverse (-)
48998152 .. 48999075
924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG377000.1

Sequence Viewer

Length: 549 bp
ATGGAGAACGCAGTGGTGATGATGGACTTGCAGGCGTTGCCGGAAGGCTGCATAGCCAACGTGATCTTGCTGACGACTCCTCGAGACGCTTGCAAACTATCTTTGATTTCAAGGAATTTCAAGTCGGCGGCGGACTCCGACGCGGTTTGGATCAAGTTCCTTCCGCATGAGACCAGAGAGATCCTATCCCACTCCGAATCTGCAATCCACGCCAAATCCAAGAAGGAGCTTTACTTCACTCTCTCTCACAACCCCATCCTCATCGACGATAGCAAGATGGGAATTGTGATTTGTAAATTCGGATTGGAATTTTTTGTTGGTATTGATTTGATTCGAAGATGTTGCTCTACAAGCAGAAGAAGAGTCCCAAGGGCAACAAGTGCCGGCCCGATCCGATTTGTCGTCAACGAGGAGATGCCTGTCGCTGCGGTCATCGATACCGCCTTGAAATCCTATGCTCCTAAAGGTCGCCTTCCGATTCTCGGCTCTGATCGCAATGAGTTCATGCTCTACTGCCTCACTGCAGGACCTGATGCTTTGAGTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

182

Amino Acids

20.11

Weight (kDa)

8.23

Isoelectric Point (pI)

47.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 126 - 182 5.1e-13 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020000)

Species Orthologous Gene IDs
rosa_laevigata RLG00000006348 RLG00000009287 RLG00000011184
rosa_multiflora Rmu_sc0000058.1_g000008 Rmu_sc0003382.1_g000029 Rmu_sc0004790.1_g000002
rosa_samantha Rh7AG377000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 143
AciI CCGC 6 cut(s) 128, 131, 143, 164, 428, 441
AclWI GGATC 3 cut(s) 158, 175, 385
AcsI RAATTY 3 cut(s) 115, 296, 308
AfiI CCNNNNNNNGG 1 cut(s) 482
AgsI TTSAA 3 cut(s) 111, 121, 448
AjuI GAANNNNNNNTTGG 2 cut(s) 300, 332
AluBI AGCT 1 cut(s) 229
AluI AGCT 1 cut(s) 229
Alw26I GTCTC 2 cut(s) 78, 164
AlwI GGATC 3 cut(s) 158, 175, 385
AlwNI CAGNNNCTG 1 cut(s) 530
Ama87I CYCGRG 1 cut(s) 81
AoxI GGCC 1 cut(s) 385
ApeKI GCWGC 2 cut(s) 48, 425
ApoI RAATTY 3 cut(s) 115, 296, 308
AspS9I GGNCC 2 cut(s) 386, 527
AsuHPI GGTGA 1 cut(s) 28
AsuII TTCGAA 1 cut(s) 334
AvaI CYCGRG 1 cut(s) 81
AvaII GGWCC 1 cut(s) 527
BarI GAAGNNNNNNTAC 2 cut(s) 215, 247
BbvI GCAGC 2 cut(s) 35, 412
BccI CCATC 3 cut(s) 16, 263, 271
BcgI CGANNNNNNTGC 4 cut(s) 72, 106, 324, 358
BcoDI GTCTC 2 cut(s) 78, 164
BfmI CTRYAG 1 cut(s) 522
BisI GCNGC 3 cut(s) 49, 129, 426
BlsI GCNGC 3 cut(s) 50, 130, 427
Bme18I GGWCC 1 cut(s) 527
BmeT110I CYCGRG 1 cut(s) 81
BmgT120I GGNCC 2 cut(s) 386, 527
BmsI GCATC 2 cut(s) 405, 523
Bpu14I TTCGAA 1 cut(s) 334
Bsa29I ATCGAT 1 cut(s) 435
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 368
Bsc4I CCNNNNNNNGG 1 cut(s) 482
Bse118I RCCGGY 1 cut(s) 383
Bse3DI GCAATG 1 cut(s) 502
BseCI ATCGAT 1 cut(s) 435
BseDI CCNNGG 1 cut(s) 368
BseGI GGATG 1 cut(s) 255
BseLI CCNNNNNNNGG 1 cut(s) 482
BseMI GCAATG 1 cut(s) 502
BseRI GAGGAG 2 cut(s) 69, 425
BseXI GCAGC 2 cut(s) 35, 412
Bsh1236I CGCG 1 cut(s) 143
BshFI GGCC 1 cut(s) 387
BshVI ATCGAT 1 cut(s) 435
BsiHKCI CYCGRG 1 cut(s) 81
BsiSI CCGG 2 cut(s) 41, 384
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 1 cut(s) 482
BsmAI GTCTC 2 cut(s) 78, 164
BsmBI CGTCTC 1 cut(s) 78
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 1 cut(s) 387
Bso31I GGTCTC 1 cut(s) 164
BsoBI CYCGRG 1 cut(s) 81
Bsp119I TTCGAA 1 cut(s) 334
Bsp143I GATC 5 cut(s) 63, 150, 180, 390, 490
BspACI CCGC 6 cut(s) 128, 131, 143, 164, 428, 441
BspANI GGCC 1 cut(s) 387
BspDI ATCGAT 1 cut(s) 435
BspFNI CGCG 1 cut(s) 143
BspMAI CTGCAG 1 cut(s) 526
BspPI GGATC 3 cut(s) 158, 175, 385
BspT104I TTCGAA 1 cut(s) 334
BspTNI GGTCTC 1 cut(s) 164
BsrDI GCAATG 1 cut(s) 502
BsrFI RCCGGY 1 cut(s) 383
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 1 cut(s) 368
BssMI GATC 5 cut(s) 63, 150, 180, 390, 490
BssT1I CCWWGG 1 cut(s) 368
Bst6I CTCTTC 1 cut(s) 355
BstAPI GCANNNNNTGC 2 cut(s) 37, 380
BstBI TTCGAA 1 cut(s) 334
BstC8I GCNNGC 3 cut(s) 33, 91, 385
BstF5I GGATG 1 cut(s) 255
BstFNI CGCG 1 cut(s) 143
BstKTI GATC 5 cut(s) 66, 153, 183, 393, 493
BstMAI GTCTC 2 cut(s) 78, 164
BstMBI GATC 5 cut(s) 63, 150, 180, 390, 490
BstMWI GCNNNNNNNGC 5 cut(s) 37, 209, 351, 380, 492
BstSFI CTRYAG 1 cut(s) 522
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 2 cut(s) 35, 412
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
Bsu15I ATCGAT 1 cut(s) 435
BsuRI GGCC 1 cut(s) 387
BsuTUI ATCGAT 1 cut(s) 435
BtsCI GGATG 1 cut(s) 255
BtsI GCAGTG 2 cut(s) 18, 519
BtsIMutI CAGTG 2 cut(s) 18, 519
Cac8I GCNNGC 3 cut(s) 33, 91, 385
CaiI CAGNNNCTG 1 cut(s) 530
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 2 cut(s) 386, 527
ClaI ATCGAT 1 cut(s) 435
CseI GACGC 2 cut(s) 95, 149
CviAII CATG 3 cut(s) 167, 505, 546
CviJI RGCY 5 cut(s) 48, 56, 229, 387, 486
CviKI_1 RGCY 5 cut(s) 48, 56, 229, 387, 486
DpnI GATC 5 cut(s) 65, 152, 182, 392, 492
DpnII GATC 5 cut(s) 63, 150, 180, 390, 490
Eam1104I CTCTTC 1 cut(s) 355
EarI CTCTTC 1 cut(s) 355
EciI GGCGGA 1 cut(s) 146
Eco130I CCWWGG 1 cut(s) 368
Eco31I GGTCTC 1 cut(s) 164
Eco47I GGWCC 1 cut(s) 527
Eco88I CYCGRG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 527
EcoT14I CCWWGG 1 cut(s) 368
ErhI CCWWGG 1 cut(s) 368
Esp3I CGTCTC 1 cut(s) 78
FaeI CATG 3 cut(s) 170, 508, 549
FaiI YATR 5 cut(s) 53, 168, 456, 506, 547
FalI AAGNNNNNCTT 2 cut(s) 456, 488
FaqI GGGAC 1 cut(s) 350
FatI CATG 3 cut(s) 166, 504, 545
Fnu4HI GCNGC 3 cut(s) 49, 129, 426
FokI GGATG 1 cut(s) 242
Fsp4HI GCNGC 3 cut(s) 49, 129, 426
GluI GCNGC 3 cut(s) 49, 129, 426
HaeIII GGCC 1 cut(s) 387
HapII CCGG 2 cut(s) 41, 384
HgaI GACGC 2 cut(s) 95, 149
Hin1II CATG 3 cut(s) 170, 508, 549
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HinfI GANTC 7 cut(s) 76, 134, 197, 331, 363, 478, 541
HpaII CCGG 2 cut(s) 41, 384
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 1 cut(s) 406
Hpy188I TCNGA 6 cut(s) 139, 196, 302, 395, 477, 490
Hpy188III TCNNGA 1 cut(s) 83
Hpy8I GTNNAC 1 cut(s) 406
Hpy99I CGWCG 2 cut(s) 143, 269
HpyAV CCTTC 4 cut(s) 38, 170, 217, 482
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 5 cut(s) 31, 51, 93, 203, 524
HpyF10VI GCNNNNNNNGC 5 cut(s) 37, 209, 351, 380, 492
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 3 cut(s) 170, 508, 549
KroI GCCGGC 1 cut(s) 383
KroNI GCCGGC 1 cut(s) 385
Kzo9I GATC 5 cut(s) 63, 150, 180, 390, 490
LmnI GCTCC 2 cut(s) 226, 463
LpnPI CCDG 7 cut(s) 17, 54, 187, 397, 432, 510, 543
Lsp1109I GCAGC 2 cut(s) 35, 412
LweI GCATC 2 cut(s) 405, 523
MaeII ACGT 1 cut(s) 60
MalI GATC 5 cut(s) 65, 152, 182, 392, 492
MboI GATC 5 cut(s) 63, 150, 180, 390, 490
MboII GAAGA 3 cut(s) 348, 369, 372
MflI RGATCY 1 cut(s) 180
MluCI AATT 4 cut(s) 115, 282, 296, 308
MlyI GAGTC 3 cut(s) 70, 128, 372
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 4 cut(s) 90, 269, 403, 527
MroNI GCCGGC 1 cut(s) 383
MspI CCGG 2 cut(s) 41, 384
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 5 cut(s) 37, 209, 351, 380, 492
NaeI GCCGGC 1 cut(s) 385
NdeII GATC 5 cut(s) 63, 150, 180, 390, 490
NgoMIV GCCGGC 1 cut(s) 383
NlaIII CATG 3 cut(s) 170, 508, 549
NmeAIII GCCGAG 1 cut(s) 462
NspV TTCGAA 1 cut(s) 334
PaeR7I CTCGAG 1 cut(s) 81
PdiI GCCGGC 1 cut(s) 385
PfeI GAWTC 3 cut(s) 197, 331, 478
PkrI GCNGC 3 cut(s) 50, 130, 427
PleI GAGTC 3 cut(s) 70, 128, 371
PpsI GAGTC 3 cut(s) 70, 128, 371
PpuMI RGGWCCY 1 cut(s) 527
Psp5II RGGWCCY 1 cut(s) 527
PspPI GGNCC 2 cut(s) 386, 527
PspPPI RGGWCCY 1 cut(s) 527
PstI CTGCAG 1 cut(s) 526
PstNI CAGNNNCTG 1 cut(s) 530
PsuI RGATCY 1 cut(s) 180
SatI GCNGC 3 cut(s) 49, 129, 426
Sau3AI GATC 5 cut(s) 63, 150, 180, 390, 490
Sau96I GGNCC 2 cut(s) 386, 527
SchI GAGTC 3 cut(s) 70, 128, 372
SetI ASST 4 cut(s) 63, 231, 469, 532
SfaNI GCATC 2 cut(s) 405, 523
SfcI CTRYAG 1 cut(s) 522
Sfr274I CTCGAG 1 cut(s) 81
SfuI TTCGAA 1 cut(s) 334
SinI GGWCC 1 cut(s) 527
SlaI CTCGAG 1 cut(s) 81
SmlI CTYRAG 1 cut(s) 81
SmoI CTYRAG 1 cut(s) 81
Sse9I AATT 4 cut(s) 115, 282, 296, 308
SsiI CCGC 6 cut(s) 128, 131, 143, 164, 428, 441
StyI CCWWGG 1 cut(s) 368
TaiI ACGT 1 cut(s) 63
TaqI TCGA 4 cut(s) 82, 264, 334, 435
TasI AATT 4 cut(s) 115, 282, 296, 308
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 3 cut(s) 197, 331, 478
TscAI CASTG 2 cut(s) 18, 526
TseI GCWGC 2 cut(s) 48, 425
TspDTI ATGAA 1 cut(s) 493
TspRI CASTG 2 cut(s) 18, 526
VpaK11BI GGWCC 1 cut(s) 527
XapI RAATTY 3 cut(s) 115, 296, 308
XhoI CTCGAG 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.