RLG00000006465

root meristem growth factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0017656
Physical Location & Seq
Reverse (-)
7460028 .. 7460814
787 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006465

Sequence Viewer

Length: 423 bp
ATGGGTCCTGTGAAGTGTGCTTACTTAGCACTGCTGCTGTTTCTCTCTGCTGCATGCCTGTGGAAGACCTCCGACGCACATTCCCCTCGTCAATTAGAGGGGAATGCTGCTGTGACAAGAATTCAAGCTGTTTCGACCGTAAATGGAGATCATATTCAAATGGTTAGTCCTCCTGATATTGCTACCAAGGAGAACAGGCTAGGAGGAAGGAAACTGGCGGTTCAGAAAGTTAAAGCGGTGAATGGTGTAAGGAGCTCAAACCAGCTTGAAGGGAACTGTGGTGGTGATCAAGTGAAGCAGGGTAGTCATGTAGAGTGTAACCAACAAAGTGGTACTGGATTTGAGGGAGTTGATGAGACTGGTTTTGTAGCTTTTAATGAGGATTACCATGCTCCAAGGCACCACCCGCCTAAGAACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

15.02

Weight (kDa)

7.74

Isoelectric Point (pI)

38.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g29040
malus_domestica MD13G1055500.v1.1
prunus_persica Prupe.1G295400_v2.0.a1
rosa_laevigata RLG00000006465
rosa_multiflora Rmu_ssc0000006.1_g000002
rosa_roxburghii Rroxscaffold_5G00378210
rosa_rugosa Rorug04G0298300
rosa_samantha Rh4AG353200 Rh4BG362800 Rh4CG377000 Rh4DG356700
rosa_wichuraiana Rw4G030940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 399
AciI CCGC 3 cut(s) 218, 236, 407
AcsI RAATTY 1 cut(s) 120
AfaI GTAC 1 cut(s) 334
AgsI TTSAA 3 cut(s) 125, 158, 269
AluBI AGCT 4 cut(s) 128, 255, 265, 371
AluI AGCT 4 cut(s) 128, 255, 265, 371
Alw21I GWGCWC 1 cut(s) 257
Alw26I GTCTC 1 cut(s) 350
ApeKI GCWGC 3 cut(s) 34, 50, 107
ApoI RAATTY 1 cut(s) 120
ArsI GACNNNNNNTTYG 2 cut(s) 151, 183
AspS9I GGNCC 1 cut(s) 5
AsuHPI GGTGA 2 cut(s) 250, 296
AvaII GGWCC 1 cut(s) 5
BanI GGYRCC 1 cut(s) 399
BanII GRGCYC 1 cut(s) 257
BarI GAAGNNNNNNTAC 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 71
Bbv12I GWGCWC 1 cut(s) 257
BbvI GCAGC 3 cut(s) 21, 37, 94
BclI TGATCA 1 cut(s) 286
BcoDI GTCTC 1 cut(s) 350
BfaI CTAG 1 cut(s) 200
BisI GCNGC 3 cut(s) 35, 51, 108
BlsI GCNGC 3 cut(s) 36, 52, 109
Bme18I GGWCC 1 cut(s) 5
BmgT120I GGNCC 1 cut(s) 5
BmiI GGNNCC 2 cut(s) 6, 401
BpiI GAAGAC 1 cut(s) 71
BsaJI CCNNGG 2 cut(s) 186, 395
Bse1I ACTGG 3 cut(s) 219, 340, 364
BseDI CCNNGG 2 cut(s) 186, 395
BseNI ACTGG 3 cut(s) 219, 340, 364
BseXI GCAGC 3 cut(s) 21, 37, 94
Bsh1285I CGRYCG 1 cut(s) 138
BshNI GGYRCC 1 cut(s) 399
BsiEI CGRYCG 1 cut(s) 138
BsiHKAI GWGCWC 1 cut(s) 257
BsmAI GTCTC 1 cut(s) 350
BsmI GAATGC 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 257
Bsp143I GATC 2 cut(s) 148, 286
BspACI CCGC 3 cut(s) 218, 236, 407
BspLI GGNNCC 2 cut(s) 6, 401
BspT107I GGYRCC 1 cut(s) 399
BsrI ACTGG 3 cut(s) 219, 340, 364
BssECI CCNNGG 2 cut(s) 186, 395
BssMI GATC 2 cut(s) 148, 286
BssT1I CCWWGG 2 cut(s) 186, 395
Bst4CI ACNGT 2 cut(s) 139, 278
BstC8I GCNNGC 1 cut(s) 55
BstDEI CTNAG 2 cut(s) 25, 411
BstKTI GATC 2 cut(s) 151, 289
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 2 cut(s) 148, 286
BstMCI CGRYCG 1 cut(s) 138
BstMWI GCNNNNNNNGC 2 cut(s) 26, 406
BstNSI RCATGY 1 cut(s) 57
BstV1I GCAGC 3 cut(s) 21, 37, 94
BstV2I GAAGAC 1 cut(s) 71
BstXI CCANNNNNNTGG 1 cut(s) 329
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 29
Cac8I GCNNGC 1 cut(s) 55
Cfr13I GGNCC 1 cut(s) 5
CseI GACGC 1 cut(s) 83
Csp6I GTAC 1 cut(s) 333
CviAII CATG 3 cut(s) 54, 308, 389
CviJI RGCY 5 cut(s) 128, 199, 255, 265, 371
CviKI_1 RGCY 5 cut(s) 128, 199, 255, 265, 371
CviQI GTAC 1 cut(s) 333
DdeI CTNAG 2 cut(s) 25, 411
DpnI GATC 2 cut(s) 150, 288
DpnII GATC 2 cut(s) 148, 286
Ecl136II GAGCTC 1 cut(s) 255
Eco130I CCWWGG 2 cut(s) 186, 395
Eco24I GRGCYC 1 cut(s) 257
Eco47I GGWCC 1 cut(s) 5
Eco53kI GAGCTC 1 cut(s) 255
EcoICRI GAGCTC 1 cut(s) 255
EcoO109I RGGNCCY 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 120
EcoT14I CCWWGG 2 cut(s) 186, 395
EcoT38I GRGCYC 1 cut(s) 257
ErhI CCWWGG 2 cut(s) 186, 395
FaeI CATG 3 cut(s) 57, 311, 392
FaiI YATR 4 cut(s) 55, 153, 309, 390
FatI CATG 3 cut(s) 53, 307, 388
FauI CCCGC 1 cut(s) 414
FbaI TGATCA 1 cut(s) 286
Fnu4HI GCNGC 3 cut(s) 35, 51, 108
FriOI GRGCYC 1 cut(s) 257
Fsp4HI GCNGC 3 cut(s) 35, 51, 108
FspBI CTAG 1 cut(s) 200
GluI GCNGC 3 cut(s) 35, 51, 108
HgaI GACGC 1 cut(s) 83
Hin1II CATG 3 cut(s) 57, 311, 392
HphI GGTGA 2 cut(s) 250, 296
Hpy188I TCNGA 2 cut(s) 73, 225
Hpy188III TCNNGA 1 cut(s) 173
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 2 cut(s) 201, 263
HpyCH4III ACNGT 2 cut(s) 139, 278
HpyCH4V TGCA 1 cut(s) 53
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 406
HpyF3I CTNAG 2 cut(s) 25, 411
Hsp92II CATG 3 cut(s) 57, 311, 392
Ksp22I TGATCA 1 cut(s) 286
Kzo9I GATC 2 cut(s) 148, 286
LmnI GCTCC 2 cut(s) 252, 397
LpnPI CCDG 9 cut(s) 21, 71, 181, 186, 200, 275, 284, 321, 345
Lsp1109I GCAGC 3 cut(s) 21, 37, 94
MaeI CTAG 1 cut(s) 200
MaeIII GTNAC 2 cut(s) 112, 317
MalI GATC 2 cut(s) 150, 288
MboI GATC 2 cut(s) 148, 286
MboII GAAGA 1 cut(s) 76
MhlI GDGCHC 1 cut(s) 257
MluCI AATT 2 cut(s) 92, 120
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 7 cut(s) 79, 91, 96, 180, 197, 337, 373
MseI TTAA 2 cut(s) 231, 375
MslI CAYNNNNRTG 1 cut(s) 58
Mva1269I GAATGC 1 cut(s) 109
MwoI GCNNNNNNNGC 2 cut(s) 26, 406
NdeII GATC 2 cut(s) 148, 286
NlaIII CATG 3 cut(s) 57, 311, 392
NlaIV GGNNCC 2 cut(s) 6, 401
NmuCI GTSAC 1 cut(s) 112
NspI RCATGY 1 cut(s) 57
PaeI GCATGC 1 cut(s) 57
PctI GAATGC 1 cut(s) 109
PkrI GCNGC 3 cut(s) 36, 52, 109
PpuMI RGGWCCY 1 cut(s) 5
Psp124BI GAGCTC 1 cut(s) 257
Psp5II RGGWCCY 1 cut(s) 5
PspN4I GGNNCC 2 cut(s) 6, 401
PspPI GGNCC 1 cut(s) 5
PspPPI RGGWCCY 1 cut(s) 5
RsaI GTAC 1 cut(s) 334
RsaNI GTAC 1 cut(s) 333
RseI CAYNNNNRTG 1 cut(s) 58
SacI GAGCTC 1 cut(s) 257
SaqAI TTAA 2 cut(s) 231, 375
SatI GCNGC 3 cut(s) 35, 51, 108
Sau3AI GATC 2 cut(s) 148, 286
Sau96I GGNCC 1 cut(s) 5
SduI GDGCHC 1 cut(s) 257
SetI ASST 5 cut(s) 71, 130, 257, 267, 373
SinI GGWCC 1 cut(s) 5
SmiMI CAYNNNNRTG 1 cut(s) 58
SphI GCATGC 1 cut(s) 57
Sse9I AATT 2 cut(s) 92, 120
SsiI CCGC 3 cut(s) 218, 236, 407
SspMI CTAG 1 cut(s) 200
SstI GAGCTC 1 cut(s) 257
StyI CCWWGG 2 cut(s) 186, 395
TaaI ACNGT 2 cut(s) 139, 278
TaqI TCGA 1 cut(s) 134
TasI AATT 2 cut(s) 92, 120
Tru1I TTAA 2 cut(s) 231, 375
Tru9I TTAA 2 cut(s) 231, 375
TscAI CASTG 1 cut(s) 36
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 3 cut(s) 34, 50, 107
Tsp45I GTSAC 1 cut(s) 112
TspRI CASTG 1 cut(s) 36
VpaK11BI GGWCC 1 cut(s) 5
XapI RAATTY 1 cut(s) 120
XceI RCATGY 1 cut(s) 57
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.