RLG00000008563

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0017569
Physical Location & Seq
Reverse (-)
32182593 .. 32184630
2038 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008563

Sequence Viewer

Length: 660 bp
ATGGTGGGCTTTGACGGTGGGCTCGAGTTCGGTGAAGATTACGCCTGGGACATGCTTGCCGGCACCGATCTCGGAGAAGATGGTGTTGAAGGCGTCGTGGCTCTGCATCTGGCAGTCCGGCTGGGGGGGGAAGGGGGAGAGAGAGAGAGAATCGGTCACTGCCTCCAAAAAGCATACGAAAAAGGAGAAATGGCAGCAACGGTAGCACGAGTAACTCTGGATCACATTGCCAGACAATCCAACGACATTAGGCGCCTCGCGGATTTCTACATAGAGACATTTGGATTCGAGGAGGTAGAGAGCCCAAAGATTGGGCCACTCAAGGTGATATGGCTGACTTTACCCTCAGCTTTTAGTATTCACCTTATTGAGTCCAACCCAAGTTACCCACCTAACCAGAGGAACGACACATCGCTGGGAGCCGACCCAGCCCGTATTCCCAGAGGCCACCATATGTGCTTCTCTGTCTCCAATTTCGAAACCTTTGTGCAAACACTCAAGGAAAAAAGAATTCAGATTGCTCATAGGCCAGTTCAATTTAATCCAAATGGCAGTAAGAAGATCGACCAGGTCTTTTTCTTTGATCCAGATGGCAACGCTTTGGAAGTTTCTGACTCCACCGAACTACAATGGAATGCTCGAAATGGTAATGCAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase Glyoxalase_4

Protein Analysis

220

Amino Acids

24.32

Weight (kDa)

5.0

Isoelectric Point (pI)

38.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 73 - 203 2.3e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017569)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03680 FvH4_1g03680
rosa_chinensis RchiOBHm_Chr4g0409411
rosa_laevigata RLG00000008020 RLG00000008563
rosa_multiflora Rmu_sc0001616.1_g000039
rosa_roxburghii Rroxscaffold_5G00353930
rosa_rugosa Rorug04G0093600 Rorug04G0093900
rosa_samantha Rh4BG155300 Rh4CG165400
rosa_wichuraiana Rw4G012780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 62, 252
AccB7I CCANNNNNTGG 1 cut(s) 311
AccII CGCG 1 cut(s) 260
AciI CCGC 1 cut(s) 260
AclWI GGATC 2 cut(s) 228, 578
AcsI RAATTY 1 cut(s) 510
AcyI GRCGYC 2 cut(s) 93, 253
AfiI CCNNNNNNNGG 2 cut(s) 124, 311
AgsI TTSAA 2 cut(s) 89, 536
AjnI CCWGG 2 cut(s) 44, 567
AluBI AGCT 1 cut(s) 350
AluI AGCT 1 cut(s) 350
Alw26I GTCTC 2 cut(s) 269, 472
AlwI GGATC 2 cut(s) 228, 578
Ama87I CYCGRG 1 cut(s) 23
AoxI GGCC 3 cut(s) 314, 445, 527
ApeKI GCWGC 1 cut(s) 194
ApoI RAATTY 1 cut(s) 510
AspLEI GCGC 1 cut(s) 255
AspS9I GGNCC 1 cut(s) 314
AsuHPI GGTGA 3 cut(s) 44, 337, 353
AsuII TTCGAA 1 cut(s) 477
AvaI CYCGRG 1 cut(s) 23
BanI GGYRCC 2 cut(s) 62, 252
BanII GRGCYC 2 cut(s) 24, 305
BauI CACGAG 1 cut(s) 207
BbvCI CCTCAGC 1 cut(s) 346
BbvI GCAGC 1 cut(s) 206
BccI CCATC 2 cut(s) 74, 584
BcgI CGANNNNNNTGC 2 cut(s) 52, 86
BciT130I CCWGG 2 cut(s) 46, 569
BcoDI GTCTC 2 cut(s) 269, 472
BfoI RGCGCY 1 cut(s) 256
BisI GCNGC 1 cut(s) 195
BlsI GCNGC 1 cut(s) 196
Bme1390I CCNGG 2 cut(s) 46, 569
BmeT110I CYCGRG 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 314
BmiI GGNNCC 3 cut(s) 64, 254, 421
BmrFI CCNGG 2 cut(s) 46, 569
BmsI GCATC 1 cut(s) 115
Bpu10I CCTNAGC 1 cut(s) 346
Bpu14I TTCGAA 1 cut(s) 477
BpuEI CTTGAG 2 cut(s) 305, 482
BsaHI GRCGYC 2 cut(s) 93, 253
BsaJI CCNNGG 1 cut(s) 45
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 311
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 1 cut(s) 530
Bse3DI GCAATG 1 cut(s) 225
BseBI CCWGG 2 cut(s) 46, 569
BseDI CCNNGG 1 cut(s) 45
BseLI CCNNNNNNNGG 2 cut(s) 124, 311
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 1 cut(s) 360
BseNI ACTGG 1 cut(s) 530
BseRI GAGGAG 1 cut(s) 305
BseXI GCAGC 1 cut(s) 206
BseYI CCCAGC 3 cut(s) 121, 415, 427
Bsh1236I CGCG 1 cut(s) 260
BshFI GGCC 3 cut(s) 316, 447, 529
BshNI GGYRCC 2 cut(s) 62, 252
BsiHKCI CYCGRG 1 cut(s) 23
BsiSI CCGG 2 cut(s) 60, 118
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 2 cut(s) 124, 311
BsmAI GTCTC 2 cut(s) 269, 472
BsmFI GGGAC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 640
BsnI GGCC 3 cut(s) 316, 447, 529
BsoBI CYCGRG 1 cut(s) 23
Bsp119I TTCGAA 1 cut(s) 477
Bsp1286I GDGCHC 2 cut(s) 24, 305
Bsp143I GATC 4 cut(s) 67, 220, 561, 583
BspACI CCGC 1 cut(s) 260
BspANI GGCC 3 cut(s) 316, 447, 529
BspCNI CTCAG 1 cut(s) 359
BspFNI CGCG 1 cut(s) 260
BspLI GGNNCC 3 cut(s) 64, 254, 421
BspPI GGATC 2 cut(s) 228, 578
BspT104I TTCGAA 1 cut(s) 477
BspT107I GGYRCC 2 cut(s) 62, 252
BsrDI GCAATG 1 cut(s) 225
BsrFI RCCGGY 1 cut(s) 59
BsrI ACTGG 1 cut(s) 530
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 4 cut(s) 67, 220, 561, 583
BssNI GRCGYC 2 cut(s) 93, 253
BssSI CACGAG 1 cut(s) 207
Bst2BI CACGAG 1 cut(s) 207
Bst2UI CCWGG 2 cut(s) 46, 569
Bst4CI ACNGT 2 cut(s) 17, 202
BstACI GRCGYC 2 cut(s) 93, 253
BstBI TTCGAA 1 cut(s) 477
BstC8I GCNNGC 2 cut(s) 57, 61
BstDEI CTNAG 1 cut(s) 346
BstFNI CGCG 1 cut(s) 260
BstH2I RGCGCY 1 cut(s) 256
BstHHI GCGC 1 cut(s) 255
BstKTI GATC 4 cut(s) 70, 223, 564, 586
BstMAI GTCTC 2 cut(s) 269, 472
BstMBI GATC 4 cut(s) 67, 220, 561, 583
BstMWI GCNNNNNNNGC 2 cut(s) 203, 428
BstNI CCWGG 2 cut(s) 46, 569
BstNSI RCATGY 1 cut(s) 55
BstSCI CCNGG 2 cut(s) 44, 567
BstUI CGCG 1 cut(s) 260
BstV1I GCAGC 1 cut(s) 206
BsuRI GGCC 3 cut(s) 316, 447, 529
BtgZI GCGATG 1 cut(s) 396
BtsI GCAGTG 1 cut(s) 157
BtsIMutI CAGTG 1 cut(s) 157
Cac8I GCNNGC 2 cut(s) 57, 61
CfoI GCGC 1 cut(s) 255
Cfr10I RCCGGY 1 cut(s) 59
Cfr13I GGNCC 1 cut(s) 314
CseI GACGC 1 cut(s) 82
CsiI ACCWGGT 1 cut(s) 567
CviAII CATG 1 cut(s) 52
DdeI CTNAG 1 cut(s) 346
DinI GGCGCC 1 cut(s) 254
DpnI GATC 4 cut(s) 69, 222, 563, 585
DpnII GATC 4 cut(s) 67, 220, 561, 583
Eco24I GRGCYC 2 cut(s) 24, 305
Eco88I CYCGRG 1 cut(s) 23
EcoRI GAATTC 1 cut(s) 510
EcoRII CCWGG 2 cut(s) 44, 567
EcoT38I GRGCYC 2 cut(s) 24, 305
EgeI GGCGCC 1 cut(s) 254
EheI GGCGCC 1 cut(s) 254
FaeI CATG 1 cut(s) 55
FaiI YATR 7 cut(s) 53, 175, 272, 331, 453, 455, 525
FaqI GGGAC 1 cut(s) 62
FatI CATG 1 cut(s) 51
FauNDI CATATG 1 cut(s) 453
Fnu4HI GCNGC 1 cut(s) 195
FriOI GRGCYC 2 cut(s) 24, 305
Fsp4HI GCNGC 1 cut(s) 195
GlaI GCGC 1 cut(s) 254
GluI GCNGC 1 cut(s) 195
GsaI CCCAGC 3 cut(s) 125, 419, 431
HaeII RGCGCY 1 cut(s) 256
HaeIII GGCC 3 cut(s) 316, 447, 529
HapII CCGG 2 cut(s) 60, 118
HgaI GACGC 1 cut(s) 82
HhaI GCGC 1 cut(s) 255
Hin1I GRCGYC 2 cut(s) 93, 253
Hin1II CATG 1 cut(s) 55
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HinfI GANTC 4 cut(s) 150, 285, 371, 614
HpaII CCGG 2 cut(s) 60, 118
HphI GGTGA 3 cut(s) 44, 337, 353
Hpy188I TCNGA 3 cut(s) 74, 516, 613
Hpy188III TCNNGA 2 cut(s) 218, 587
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 2 cut(s) 83, 125
HpyCH4III ACNGT 2 cut(s) 17, 202
HpyCH4V TGCA 3 cut(s) 106, 490, 653
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 428
HpyF3I CTNAG 1 cut(s) 346
Hsp92I GRCGYC 2 cut(s) 93, 253
Hsp92II CATG 1 cut(s) 55
HspAI GCGC 1 cut(s) 253
KasI GGCGCC 1 cut(s) 252
KroI GCCGGC 1 cut(s) 59
KroNI GCCGGC 1 cut(s) 61
Kzo9I GATC 4 cut(s) 67, 220, 561, 583
LmnI GCTCC 1 cut(s) 419
Lsp1109I GCAGC 1 cut(s) 206
LweI GCATC 1 cut(s) 115
MabI ACCWGGT 1 cut(s) 567
MaeIII GTNAC 3 cut(s) 155, 211, 383
MalI GATC 4 cut(s) 69, 222, 563, 585
MboI GATC 4 cut(s) 67, 220, 561, 583
MboII GAAGA 3 cut(s) 47, 89, 571
MhlI GDGCHC 2 cut(s) 24, 305
MluCI AATT 3 cut(s) 472, 510, 536
Mly113I GGCGCC 1 cut(s) 253
MlyI GAGTC 2 cut(s) 380, 608
MmeI TCCRAC 2 cut(s) 264, 399
MnlI CCTC 7 cut(s) 173, 266, 283, 286, 355, 393, 437
MroNI GCCGGC 1 cut(s) 59
MseI TTAA 1 cut(s) 540
MspI CCGG 2 cut(s) 60, 118
MspR9I CCNGG 2 cut(s) 46, 569
Mva1269I GAATGC 1 cut(s) 640
MvaI CCWGG 2 cut(s) 46, 569
MvnI CGCG 1 cut(s) 260
MwoI GCNNNNNNNGC 2 cut(s) 203, 428
NaeI GCCGGC 1 cut(s) 61
NarI GGCGCC 1 cut(s) 253
NdeI CATATG 1 cut(s) 453
NdeII GATC 4 cut(s) 67, 220, 561, 583
NgoMIV GCCGGC 1 cut(s) 59
NlaIII CATG 1 cut(s) 55
NlaIV GGNNCC 3 cut(s) 64, 254, 421
NmuCI GTSAC 1 cut(s) 155
NspI RCATGY 1 cut(s) 55
NspV TTCGAA 1 cut(s) 477
PaeR7I CTCGAG 1 cut(s) 23
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PctI GAATGC 1 cut(s) 640
PdiI GCCGGC 1 cut(s) 61
PfeI GAWTC 2 cut(s) 150, 285
PflFI GACNNNGTC 1 cut(s) 569
PflMI CCANNNNNTGG 1 cut(s) 311
PkrI GCNGC 1 cut(s) 196
PleI GAGTC 2 cut(s) 379, 608
PluTI GGCGCC 1 cut(s) 256
PpsI GAGTC 2 cut(s) 379, 608
Psp6I CCWGG 2 cut(s) 44, 567
PspFI CCCAGC 3 cut(s) 121, 415, 427
PspGI CCWGG 2 cut(s) 44, 567
PspN4I GGNNCC 3 cut(s) 64, 254, 421
PspPI GGNCC 1 cut(s) 314
PspXI VCTCGAGB 1 cut(s) 23
PsyI GACNNNGTC 1 cut(s) 569
SaqAI TTAA 1 cut(s) 540
SatI GCNGC 1 cut(s) 195
Sau3AI GATC 4 cut(s) 67, 220, 561, 583
Sau96I GGNCC 1 cut(s) 314
SchI GAGTC 2 cut(s) 380, 608
ScrFI CCNGG 2 cut(s) 46, 569
SduI GDGCHC 2 cut(s) 24, 305
SetI ASST 7 cut(s) 297, 327, 352, 366, 394, 485, 573
SexAI ACCWGGT 1 cut(s) 567
SfaNI GCATC 1 cut(s) 115
SfoI GGCGCC 1 cut(s) 254
Sfr274I CTCGAG 1 cut(s) 23
SfuI TTCGAA 1 cut(s) 477
SlaI CTCGAG 1 cut(s) 23
SmlI CTYRAG 3 cut(s) 23, 320, 497
SmoI CTYRAG 3 cut(s) 23, 320, 497
Sse9I AATT 3 cut(s) 472, 510, 536
SsiI CCGC 1 cut(s) 260
SspDI GGCGCC 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 44, 567
TaaI ACNGT 2 cut(s) 17, 202
TaqI TCGA 5 cut(s) 24, 288, 477, 564, 640
TaqII GACCGA 1 cut(s) 143
TasI AATT 3 cut(s) 472, 510, 536
TfiI GAWTC 2 cut(s) 150, 285
Tru1I TTAA 1 cut(s) 540
Tru9I TTAA 1 cut(s) 540
TscAI CASTG 1 cut(s) 164
TseFI GTSAC 1 cut(s) 155
TseI GCWGC 1 cut(s) 194
Tsp45I GTSAC 1 cut(s) 155
TspRI CASTG 1 cut(s) 164
Tth111I GACNNNGTC 1 cut(s) 569
Van91I CCANNNNNTGG 1 cut(s) 311
XapI RAATTY 1 cut(s) 510
XceI RCATGY 1 cut(s) 55
XhoI CTCGAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.