Rh4BG155300

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0017569
Physical Location & Seq
Forward (+)
27955653 .. 28028979
73327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG155300.1

Sequence Viewer

Length: 417 bp
ATGGCAGCAATGGTAGCAGGAGTAACTCTGGATCACATTGCCAGACAATCCAACGACATTAGGCGCCTCGCCGATTTCTACATAGAGACATTTGGATTCGAGGGGGTAGAGAGCCCAAAGATTGGGCCACTCAAGGTAATATGGCTGAGTTTACCCTCAGGTTTTACTATACACCTTATTGAGTCCAACCCAAGTTACCCACCTAACCAGAGGAACGACACATCACCGGGAGCCGACCCAGCCCGTATTCCCAGAGGCCACCATATGTGCTTCTGTGTCTCCAATTTCGAAACCTTTGTGCAAACACTCAAGGAAAAGGGAATTCAGATTGCTCATAGGCCAGTGCAATTTAATCCAAATGGCAGTAAGAAGATCGACCAGGTCTTTTTCTTTGATCCAGATGGTAAGCTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase Glyoxalase_4

Protein Analysis

138

Amino Acids

15.47

Weight (kDa)

6.96

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 10 - 136 1.7e-08 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 11 - 117 2.9e-06 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017569)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03680 FvH4_1g03680
rosa_chinensis RchiOBHm_Chr4g0409411
rosa_laevigata RLG00000008020 RLG00000008563
rosa_multiflora Rmu_sc0001616.1_g000039
rosa_roxburghii Rroxscaffold_5G00353930
rosa_rugosa Rorug04G0093600 Rorug04G0093900
rosa_samantha Rh4BG155300 Rh4CG165400
rosa_wichuraiana Rw4G012780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 63
AccB7I CCANNNNNTGG 1 cut(s) 122
AclWI GGATC 2 cut(s) 39, 389
AcsI RAATTY 1 cut(s) 321
AcyI GRCGYC 1 cut(s) 64
AfiI CCNNNNNNNGG 1 cut(s) 122
AjnI CCWGG 1 cut(s) 378
AluBI AGCT 1 cut(s) 409
AluI AGCT 1 cut(s) 409
Alw26I GTCTC 2 cut(s) 80, 283
AlwI GGATC 2 cut(s) 39, 389
AoxI GGCC 3 cut(s) 125, 256, 338
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 321
AspLEI GCGC 1 cut(s) 66
AspS9I GGNCC 1 cut(s) 125
AsuC2I CCSGG 1 cut(s) 228
AsuHPI GGTGA 1 cut(s) 216
AsuII TTCGAA 1 cut(s) 288
AxyI CCTNAGG 1 cut(s) 157
BanI GGYRCC 1 cut(s) 63
BanII GRGCYC 1 cut(s) 116
BbvI GCAGC 1 cut(s) 17
BccI CCATC 1 cut(s) 395
BciT130I CCWGG 1 cut(s) 380
BcnI CCSGG 1 cut(s) 228
BcoDI GTCTC 2 cut(s) 80, 283
BfoI RGCGCY 1 cut(s) 67
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 228, 380
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 2 cut(s) 65, 232
BmrFI CCNGG 2 cut(s) 228, 380
Bpu14I TTCGAA 1 cut(s) 288
BpuEI CTTGAG 2 cut(s) 116, 293
BpuMI CCSGG 1 cut(s) 228
BsaHI GRCGYC 1 cut(s) 64
Bsc4I CCNNNNNNNGG 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 341
Bse21I CCTNAGG 1 cut(s) 157
Bse3DI GCAATG 2 cut(s) 15, 36
BseBI CCWGG 1 cut(s) 380
BseLI CCNNNNNNNGG 1 cut(s) 122
BseMI GCAATG 2 cut(s) 15, 36
BseMII CTCAG 2 cut(s) 137, 171
BseNI ACTGG 1 cut(s) 341
BseXI GCAGC 1 cut(s) 17
BseYI CCCAGC 1 cut(s) 238
BshFI GGCC 3 cut(s) 127, 258, 340
BshNI GGYRCC 1 cut(s) 63
BsiSI CCGG 1 cut(s) 227
BslI CCNNNNNNNGG 1 cut(s) 122
BsmAI GTCTC 2 cut(s) 80, 283
BsnI GGCC 3 cut(s) 127, 258, 340
Bsp119I TTCGAA 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 116
Bsp143I GATC 3 cut(s) 31, 372, 394
BspANI GGCC 3 cut(s) 127, 258, 340
BspCNI CTCAG 2 cut(s) 138, 170
BspLI GGNNCC 2 cut(s) 65, 232
BspPI GGATC 2 cut(s) 39, 389
BspT104I TTCGAA 1 cut(s) 288
BspT107I GGYRCC 1 cut(s) 63
BsrDI GCAATG 2 cut(s) 15, 36
BsrI ACTGG 1 cut(s) 341
BssMI GATC 3 cut(s) 31, 372, 394
BssNI GRCGYC 1 cut(s) 64
Bst2UI CCWGG 1 cut(s) 380
BstACI GRCGYC 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 288
BstDEI CTNAG 2 cut(s) 146, 157
BstH2I RGCGCY 1 cut(s) 67
BstHHI GCGC 1 cut(s) 66
BstKTI GATC 3 cut(s) 34, 375, 397
BstMAI GTCTC 2 cut(s) 80, 283
BstMBI GATC 3 cut(s) 31, 372, 394
BstMWI GCNNNNNNNGC 2 cut(s) 14, 239
BstNI CCWGG 1 cut(s) 380
BstSCI CCNGG 2 cut(s) 226, 378
BstV1I GCAGC 1 cut(s) 17
Bsu36I CCTNAGG 1 cut(s) 157
BsuRI GGCC 3 cut(s) 127, 258, 340
BtsIMutI CAGTG 1 cut(s) 348
CfoI GCGC 1 cut(s) 66
Cfr13I GGNCC 1 cut(s) 125
CsiI ACCWGGT 1 cut(s) 378
CviJI RGCY 8 cut(s) 114, 127, 145, 233, 242, 258, 340, 409
CviKI_1 RGCY 8 cut(s) 114, 127, 145, 233, 242, 258, 340, 409
DdeI CTNAG 2 cut(s) 146, 157
DinI GGCGCC 1 cut(s) 65
DpnI GATC 3 cut(s) 33, 374, 396
DpnII GATC 3 cut(s) 31, 372, 394
Eco24I GRGCYC 1 cut(s) 116
Eco81I CCTNAGG 1 cut(s) 157
EcoRI GAATTC 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 378
EcoT38I GRGCYC 1 cut(s) 116
EgeI GGCGCC 1 cut(s) 65
EheI GGCGCC 1 cut(s) 65
FaiI YATR 6 cut(s) 83, 142, 170, 264, 266, 336
FauNDI CATATG 1 cut(s) 264
Fnu4HI GCNGC 1 cut(s) 6
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 1 cut(s) 6
GlaI GCGC 1 cut(s) 65
GluI GCNGC 1 cut(s) 6
GsaI CCCAGC 1 cut(s) 242
HaeII RGCGCY 1 cut(s) 67
HaeIII GGCC 3 cut(s) 127, 258, 340
HapII CCGG 1 cut(s) 227
HhaI GCGC 1 cut(s) 66
Hin1I GRCGYC 1 cut(s) 64
Hin6I GCGC 1 cut(s) 64
HinP1I GCGC 1 cut(s) 64
HindIII AAGCTT 1 cut(s) 407
HinfI GANTC 2 cut(s) 96, 182
HpaII CCGG 1 cut(s) 227
HphI GGTGA 1 cut(s) 216
Hpy166II GTNNAC 1 cut(s) 152
Hpy188I TCNGA 1 cut(s) 327
Hpy188III TCNNGA 2 cut(s) 29, 398
Hpy8I GTNNAC 1 cut(s) 152
HpyCH4V TGCA 2 cut(s) 301, 346
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 239
HpyF3I CTNAG 2 cut(s) 146, 157
Hsp92I GRCGYC 1 cut(s) 64
HspAI GCGC 1 cut(s) 64
KasI GGCGCC 1 cut(s) 63
Kzo9I GATC 3 cut(s) 31, 372, 394
LmnI GCTCC 1 cut(s) 230
Lsp1109I GCAGC 1 cut(s) 17
MabI ACCWGGT 1 cut(s) 378
MaeIII GTNAC 2 cut(s) 22, 194
MalI GATC 3 cut(s) 33, 374, 396
MboI GATC 3 cut(s) 31, 372, 394
MboII GAAGA 1 cut(s) 382
MhlI GDGCHC 1 cut(s) 116
MluCI AATT 3 cut(s) 283, 321, 347
Mly113I GGCGCC 1 cut(s) 64
MlyI GAGTC 1 cut(s) 191
MmeI TCCRAC 2 cut(s) 75, 210
MnlI CCTC 5 cut(s) 77, 94, 166, 204, 248
MseI TTAA 1 cut(s) 351
MspI CCGG 1 cut(s) 227
MspR9I CCNGG 2 cut(s) 228, 380
MvaI CCWGG 1 cut(s) 380
MwoI GCNNNNNNNGC 2 cut(s) 14, 239
NarI GGCGCC 1 cut(s) 64
NciI CCSGG 1 cut(s) 228
NdeI CATATG 1 cut(s) 264
NdeII GATC 3 cut(s) 31, 372, 394
NlaIV GGNNCC 2 cut(s) 65, 232
NspV TTCGAA 1 cut(s) 288
PfeI GAWTC 1 cut(s) 96
PflFI GACNNNGTC 1 cut(s) 380
PflMI CCANNNNNTGG 1 cut(s) 122
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 190
PluTI GGCGCC 1 cut(s) 67
PpsI GAGTC 1 cut(s) 190
Psp6I CCWGG 1 cut(s) 378
PspFI CCCAGC 1 cut(s) 238
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 2 cut(s) 65, 232
PspPI GGNCC 1 cut(s) 125
PsyI GACNNNGTC 1 cut(s) 380
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 3 cut(s) 31, 372, 394
Sau96I GGNCC 1 cut(s) 125
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 2 cut(s) 228, 380
SduI GDGCHC 1 cut(s) 116
SetI ASST 7 cut(s) 138, 163, 177, 205, 296, 384, 411
SexAI ACCWGGT 1 cut(s) 378
SfoI GGCGCC 1 cut(s) 65
SfuI TTCGAA 1 cut(s) 288
SmlI CTYRAG 2 cut(s) 131, 308
SmoI CTYRAG 2 cut(s) 131, 308
Sse9I AATT 3 cut(s) 283, 321, 347
SspDI GGCGCC 1 cut(s) 63
StyD4I CCNGG 2 cut(s) 226, 378
TaqI TCGA 3 cut(s) 99, 288, 375
TasI AATT 3 cut(s) 283, 321, 347
TfiI GAWTC 1 cut(s) 96
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 1 cut(s) 348
TseI GCWGC 1 cut(s) 5
TspRI CASTG 1 cut(s) 348
Tth111I GACNNNGTC 1 cut(s) 380
Van91I CCANNNNNTGG 1 cut(s) 122
XapI RAATTY 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.