Rw4G012780

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017569
Physical Location & Seq
Forward (+)
29993680 .. 29997534
3855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G012780.1

Sequence Viewer

Length: 477 bp
ATGGCAGCAATGGTAGCACGAGTAACTCTGGATCACATTGCCAGACAATCCAACGACATTAGGCGCCTCGCCGATTTCTACATAGAGACATTTGGATTCGAGGAGGTAGAGAGCCCAAAGATTGGGCCACTCAAGGTAATATGGCTGAGTTTACCCTCAGGTTTTACTATACACCTTATTGAGTCCAACCCAAGTTACCCACCTAACCAGAGGAACGACACAACACCGGGAGCCGACCCAGCCCGTATTCCCAGAGGCCACCATATGTGCTTCTCTGTCTCCAATTTCGAAACCTTTGTGCAAACACTCAAGGAAAAGGGAATTCAGATTGCTCATAGGCCAGTTCAATTTAATCCAAATGGCAGTAAGAAGATCGACCAGGTCTTTTTCTTTGATCCAGATGGCAACGCTTTGGAGGTTTCCGACTCCACCTCCACGGCACTACAGTGGAGTGCTCGAAATGGTAGTGCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase Glyoxalase_4

Protein Analysis

158

Amino Acids

17.62

Weight (kDa)

6.18

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 10 - 140 3.1e-12 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 11 - 117 1.2e-06 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017569)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03680 FvH4_1g03680
rosa_chinensis RchiOBHm_Chr4g0409411
rosa_laevigata RLG00000008020 RLG00000008563
rosa_multiflora Rmu_sc0001616.1_g000039
rosa_roxburghii Rroxscaffold_5G00353930
rosa_rugosa Rorug04G0093600 Rorug04G0093900
rosa_samantha Rh4BG155300 Rh4CG165400
rosa_wichuraiana Rw4G012780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 63
AccB7I CCANNNNNTGG 1 cut(s) 122
AclWI GGATC 2 cut(s) 39, 389
AcsI RAATTY 1 cut(s) 321
AcyI GRCGYC 1 cut(s) 64
AfiI CCNNNNNNNGG 1 cut(s) 122
AgsI TTSAA 1 cut(s) 347
AjnI CCWGG 1 cut(s) 378
AleI CACNNNNGTG 1 cut(s) 445
Alw21I GWGCWC 1 cut(s) 457
Alw26I GTCTC 2 cut(s) 80, 283
AlwI GGATC 2 cut(s) 39, 389
AoxI GGCC 3 cut(s) 125, 256, 338
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 321
AspLEI GCGC 1 cut(s) 66
AspS9I GGNCC 1 cut(s) 125
AsuC2I CCSGG 1 cut(s) 228
AsuII TTCGAA 1 cut(s) 288
AxyI CCTNAGG 1 cut(s) 157
BanI GGYRCC 1 cut(s) 63
BanII GRGCYC 1 cut(s) 116
BauI CACGAG 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 457
BbvI GCAGC 1 cut(s) 17
BccI CCATC 1 cut(s) 395
BceAI ACGGC 1 cut(s) 453
BciT130I CCWGG 1 cut(s) 380
BcnI CCSGG 1 cut(s) 228
BcoDI GTCTC 2 cut(s) 80, 283
BfmI CTRYAG 1 cut(s) 443
BfoI RGCGCY 1 cut(s) 67
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 228, 380
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 2 cut(s) 65, 232
BmrFI CCNGG 2 cut(s) 228, 380
Bpu14I TTCGAA 1 cut(s) 288
BpuEI CTTGAG 2 cut(s) 116, 293
BpuMI CCSGG 1 cut(s) 228
BsaHI GRCGYC 1 cut(s) 64
BsaJI CCNNGG 1 cut(s) 435
BsaXI ACNNNNNCTCC 2 cut(s) 416, 446
Bsc4I CCNNNNNNNGG 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 341
Bse21I CCTNAGG 1 cut(s) 157
Bse3DI GCAATG 2 cut(s) 15, 36
BseBI CCWGG 1 cut(s) 380
BseDI CCNNGG 1 cut(s) 435
BseLI CCNNNNNNNGG 1 cut(s) 122
BseMI GCAATG 2 cut(s) 15, 36
BseMII CTCAG 2 cut(s) 137, 171
BseNI ACTGG 1 cut(s) 341
BseRI GAGGAG 1 cut(s) 116
BseXI GCAGC 1 cut(s) 17
BseYI CCCAGC 1 cut(s) 238
BshFI GGCC 3 cut(s) 127, 258, 340
BshNI GGYRCC 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 457
BsiSI CCGG 1 cut(s) 227
BslI CCNNNNNNNGG 1 cut(s) 122
BsmAI GTCTC 2 cut(s) 80, 283
BsnI GGCC 3 cut(s) 127, 258, 340
Bsp119I TTCGAA 1 cut(s) 288
Bsp1286I GDGCHC 2 cut(s) 116, 457
Bsp143I GATC 3 cut(s) 31, 372, 394
BspANI GGCC 3 cut(s) 127, 258, 340
BspCNI CTCAG 2 cut(s) 138, 170
BspLI GGNNCC 2 cut(s) 65, 232
BspPI GGATC 2 cut(s) 39, 389
BspT104I TTCGAA 1 cut(s) 288
BspT107I GGYRCC 1 cut(s) 63
BsrDI GCAATG 2 cut(s) 15, 36
BsrI ACTGG 1 cut(s) 341
BssECI CCNNGG 1 cut(s) 435
BssMI GATC 3 cut(s) 31, 372, 394
BssNI GRCGYC 1 cut(s) 64
BssSI CACGAG 1 cut(s) 18
Bst2BI CACGAG 1 cut(s) 18
Bst2UI CCWGG 1 cut(s) 380
Bst4CI ACNGT 1 cut(s) 447
BstACI GRCGYC 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 288
BstDEI CTNAG 2 cut(s) 146, 157
BstDSI CCRYGG 1 cut(s) 435
BstH2I RGCGCY 1 cut(s) 67
BstHHI GCGC 1 cut(s) 66
BstKTI GATC 3 cut(s) 34, 375, 397
BstMAI GTCTC 2 cut(s) 80, 283
BstMBI GATC 3 cut(s) 31, 372, 394
BstMWI GCNNNNNNNGC 2 cut(s) 14, 239
BstNI CCWGG 1 cut(s) 380
BstSCI CCNGG 2 cut(s) 226, 378
BstSFI CTRYAG 1 cut(s) 443
BstV1I GCAGC 1 cut(s) 17
Bsu36I CCTNAGG 1 cut(s) 157
BsuRI GGCC 3 cut(s) 127, 258, 340
BtgI CCRYGG 1 cut(s) 435
BtsIMutI CAGTG 1 cut(s) 452
CfoI GCGC 1 cut(s) 66
Cfr13I GGNCC 1 cut(s) 125
CsiI ACCWGGT 1 cut(s) 378
CviJI RGCY 7 cut(s) 114, 127, 145, 233, 242, 258, 340
CviKI_1 RGCY 7 cut(s) 114, 127, 145, 233, 242, 258, 340
DdeI CTNAG 2 cut(s) 146, 157
DinI GGCGCC 1 cut(s) 65
DpnI GATC 3 cut(s) 33, 374, 396
DpnII GATC 3 cut(s) 31, 372, 394
Eco24I GRGCYC 1 cut(s) 116
Eco81I CCTNAGG 1 cut(s) 157
EcoRI GAATTC 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 378
EcoT38I GRGCYC 1 cut(s) 116
EgeI GGCGCC 1 cut(s) 65
EheI GGCGCC 1 cut(s) 65
FaiI YATR 6 cut(s) 83, 142, 170, 264, 266, 336
FauNDI CATATG 1 cut(s) 264
Fnu4HI GCNGC 1 cut(s) 6
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 1 cut(s) 6
GlaI GCGC 1 cut(s) 65
GluI GCNGC 1 cut(s) 6
GsaI CCCAGC 1 cut(s) 242
HaeII RGCGCY 1 cut(s) 67
HaeIII GGCC 3 cut(s) 127, 258, 340
HapII CCGG 1 cut(s) 227
HhaI GCGC 1 cut(s) 66
Hin1I GRCGYC 1 cut(s) 64
Hin6I GCGC 1 cut(s) 64
HinP1I GCGC 1 cut(s) 64
HinfI GANTC 3 cut(s) 96, 182, 425
HpaII CCGG 1 cut(s) 227
Hpy166II GTNNAC 1 cut(s) 152
Hpy188I TCNGA 2 cut(s) 327, 424
Hpy188III TCNNGA 2 cut(s) 29, 398
Hpy8I GTNNAC 1 cut(s) 152
HpyCH4III ACNGT 1 cut(s) 447
HpyCH4V TGCA 2 cut(s) 301, 470
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 239
HpyF3I CTNAG 2 cut(s) 146, 157
Hsp92I GRCGYC 1 cut(s) 64
HspAI GCGC 1 cut(s) 64
KasI GGCGCC 1 cut(s) 63
Kzo9I GATC 3 cut(s) 31, 372, 394
LmnI GCTCC 1 cut(s) 230
Lsp1109I GCAGC 1 cut(s) 17
MabI ACCWGGT 1 cut(s) 378
MaeIII GTNAC 2 cut(s) 22, 194
MalI GATC 3 cut(s) 33, 374, 396
MboI GATC 3 cut(s) 31, 372, 394
MboII GAAGA 1 cut(s) 382
MhlI GDGCHC 2 cut(s) 116, 457
MluCI AATT 4 cut(s) 283, 321, 347, 472
Mly113I GGCGCC 1 cut(s) 64
MlyI GAGTC 2 cut(s) 191, 419
MmeI TCCRAC 3 cut(s) 75, 210, 447
MnlI CCTC 8 cut(s) 77, 94, 97, 166, 204, 248, 409, 442
MseI TTAA 1 cut(s) 351
MslI CAYNNNNRTG 1 cut(s) 445
MspI CCGG 1 cut(s) 227
MspR9I CCNGG 2 cut(s) 228, 380
MvaI CCWGG 1 cut(s) 380
MwoI GCNNNNNNNGC 2 cut(s) 14, 239
NarI GGCGCC 1 cut(s) 64
NciI CCSGG 1 cut(s) 228
NdeI CATATG 1 cut(s) 264
NdeII GATC 3 cut(s) 31, 372, 394
NlaIV GGNNCC 2 cut(s) 65, 232
NspV TTCGAA 1 cut(s) 288
OliI CACNNNNGTG 1 cut(s) 445
PfeI GAWTC 1 cut(s) 96
PflFI GACNNNGTC 1 cut(s) 380
PflMI CCANNNNNTGG 1 cut(s) 122
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 2 cut(s) 190, 419
PluTI GGCGCC 1 cut(s) 67
PpsI GAGTC 2 cut(s) 190, 419
Psp6I CCWGG 1 cut(s) 378
PspFI CCCAGC 1 cut(s) 238
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 2 cut(s) 65, 232
PspPI GGNCC 1 cut(s) 125
PsyI GACNNNGTC 1 cut(s) 380
RseI CAYNNNNRTG 1 cut(s) 445
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 3 cut(s) 31, 372, 394
Sau96I GGNCC 1 cut(s) 125
SchI GAGTC 2 cut(s) 191, 419
ScrFI CCNGG 2 cut(s) 228, 380
SduI GDGCHC 2 cut(s) 116, 457
SetI ASST 9 cut(s) 108, 138, 163, 177, 205, 296, 384, 420, 434
SexAI ACCWGGT 1 cut(s) 378
SfcI CTRYAG 1 cut(s) 443
SfoI GGCGCC 1 cut(s) 65
SfuI TTCGAA 1 cut(s) 288
SmiMI CAYNNNNRTG 1 cut(s) 445
SmlI CTYRAG 2 cut(s) 131, 308
SmoI CTYRAG 2 cut(s) 131, 308
Sse9I AATT 4 cut(s) 283, 321, 347, 472
SspDI GGCGCC 1 cut(s) 63
StyD4I CCNGG 2 cut(s) 226, 378
TaaI ACNGT 1 cut(s) 447
TaqI TCGA 4 cut(s) 99, 288, 375, 457
TasI AATT 4 cut(s) 283, 321, 347, 472
TfiI GAWTC 1 cut(s) 96
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 1 cut(s) 452
TseI GCWGC 1 cut(s) 5
TspRI CASTG 1 cut(s) 452
Tth111I GACNNNGTC 1 cut(s) 380
Van91I CCANNNNNTGG 1 cut(s) 122
XapI RAATTY 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.