RLG00000011262

isoflavone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0018227
Physical Location & Seq
Reverse (-)
8069037 .. 8069749
713 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011262

Sequence Viewer

Length: 525 bp
ATGGCTGATCCTCAGAAAGGCAAGATTTTGGTAATCGGAGCCTCCGGCTACATAGGCAAGTTCATCGTTGAAGCGAGCGCGAAGGGTAAACATCCCACCTTTGCTCTTGTTAGGGGGACACTGTACGTCAATGACCCTGCCAAGGGAAAACTCATTCAAAATTTCAAGAACTTGGGAATCAATTTGCTCTATGTATATCTTAATGACGAGCACGAGAGCTTGGTGAAGGCGATCAAACAGGTGGACGTGGTGATATCAACAGTGGGTTACTCCTTCAATGAACTCGTTGCCTTGTGGGAGAAGAAAATTGGCAAAGTACTTGAGAAGGTCTATGTCCCAGAAGAAAAACTCCACGACGATATTAGAGCGACCCCATTTCCAGAAAATGTGATTTTGGGAATCAACCACTCTGCGTTTGTGAAAGGAGATCTTACTAACTTTGAGATTGAGCCATCGATCGGATTCGAAGCTTTCGAGCTATACCCAGATGTTGAATACACTACTGTTGAGGACTACCTCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0010035 GO:0010038 GO:0016020 GO:0042221 GO:0044464 GO:0046686 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

175

Amino Acids

19.45

Weight (kDa)

5.14

Isoelectric Point (pI)

14.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 89 2.1e-20 NmrA-like family
Sacchrp_dh_NADP PF03435 9 - 96 4.7e-06 Saccharopine dehydrogenase NADP binding domain
NmrA PF05368 90 - 173 1.5e-18 NmrA-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 80
AclWI GGATC 1 cut(s) 2
AcsI RAATTY 1 cut(s) 160
AfaI GTAC 2 cut(s) 125, 318
AfiI CCNNNNNNNGG 4 cut(s) 17, 142, 143, 458
AgsI TTSAA 5 cut(s) 71, 158, 166, 277, 494
AjiI CACGTC 1 cut(s) 247
AluBI AGCT 3 cut(s) 219, 470, 478
AluI AGCT 3 cut(s) 219, 470, 478
Alw21I GWGCWC 1 cut(s) 213
AlwI GGATC 1 cut(s) 2
ApoI RAATTY 1 cut(s) 160
AspLEI GCGC 1 cut(s) 80
AsuHPI GGTGA 2 cut(s) 235, 262
AsuII TTCGAA 1 cut(s) 465
BauI CACGAG 1 cut(s) 212
Bbv12I GWGCWC 1 cut(s) 213
BccI CCATC 1 cut(s) 460
BcgI CGANNNNNNTGC 2 cut(s) 46, 80
BglII AGATCT 1 cut(s) 427
BmcAI AGTACT 1 cut(s) 318
BmgBI CACGTC 1 cut(s) 247
BmiI GGNNCC 1 cut(s) 40
Bpu14I TTCGAA 1 cut(s) 465
BpuEI CTTGAG 1 cut(s) 341
Bsa29I ATCGAT 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 141
Bsc4I CCNNNNNNNGG 4 cut(s) 17, 142, 143, 458
BseCI ATCGAT 1 cut(s) 455
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 1 cut(s) 91
BseLI CCNNNNNNNGG 4 cut(s) 17, 142, 143, 458
BseMII CTCAG 1 cut(s) 26
Bsh1236I CGCG 1 cut(s) 80
Bsh1285I CGRYCG 1 cut(s) 459
BshVI ATCGAT 1 cut(s) 455
BsiEI CGRYCG 1 cut(s) 459
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 1 cut(s) 45
BslFI GGGAC 2 cut(s) 130, 320
BslI CCNNNNNNNGG 4 cut(s) 17, 142, 143, 458
BsmFI GGGAC 2 cut(s) 130, 320
Bsp119I TTCGAA 1 cut(s) 465
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 4 cut(s) 7, 231, 427, 456
BspCNI CTCAG 1 cut(s) 25
BspDI ATCGAT 1 cut(s) 455
BspFNI CGCG 1 cut(s) 80
BspLI GGNNCC 1 cut(s) 40
BspPI GGATC 1 cut(s) 2
BspT104I TTCGAA 1 cut(s) 465
BssECI CCNNGG 1 cut(s) 141
BssMI GATC 4 cut(s) 7, 231, 427, 456
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 1 cut(s) 141
Bst2BI CACGAG 1 cut(s) 212
Bst4CI ACNGT 3 cut(s) 123, 262, 505
BstBI TTCGAA 1 cut(s) 465
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 1 cut(s) 12
BstENI CCTNNNNNAGG 1 cut(s) 15
BstF5I GGATG 1 cut(s) 91
BstFNI CGCG 1 cut(s) 80
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 4 cut(s) 10, 234, 430, 459
BstMBI GATC 4 cut(s) 7, 231, 427, 456
BstMCI CGRYCG 1 cut(s) 459
BstMWI GCNNNNNNNGC 1 cut(s) 54
BstUI CGCG 1 cut(s) 80
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
Bsu15I ATCGAT 1 cut(s) 455
BsuTUI ATCGAT 1 cut(s) 455
BtrI CACGTC 1 cut(s) 247
BtsCI GGATG 1 cut(s) 91
BtsIMutI CAGTG 2 cut(s) 119, 267
Cac8I GCNNGC 1 cut(s) 76
CfoI GCGC 1 cut(s) 80
ClaI ATCGAT 1 cut(s) 455
Csp6I GTAC 2 cut(s) 124, 317
CviJI RGCY 7 cut(s) 5, 41, 48, 219, 451, 470, 478
CviKI_1 RGCY 7 cut(s) 5, 41, 48, 219, 451, 470, 478
CviQI GTAC 2 cut(s) 124, 317
DdeI CTNAG 1 cut(s) 12
DpnI GATC 4 cut(s) 9, 233, 429, 458
DpnII GATC 4 cut(s) 7, 231, 427, 456
Eco130I CCWWGG 1 cut(s) 141
Eco32I GATATC 1 cut(s) 255
EcoNI CCTNNNNNAGG 1 cut(s) 15
EcoRV GATATC 1 cut(s) 255
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
FaiI YATR 5 cut(s) 53, 192, 196, 333, 481
FalI AAGNNNNNCTT 2 cut(s) 414, 446
FaqI GGGAC 2 cut(s) 130, 320
FokI GGATG 1 cut(s) 78
GlaI GCGC 1 cut(s) 79
HapII CCGG 1 cut(s) 45
HhaI GCGC 1 cut(s) 80
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HindIII AAGCTT 1 cut(s) 468
HinfI GANTC 3 cut(s) 177, 399, 462
HpaII CCGG 1 cut(s) 45
HphI GGTGA 2 cut(s) 235, 262
Hpy166II GTNNAC 2 cut(s) 89, 244
Hpy188I TCNGA 3 cut(s) 15, 38, 461
Hpy188III TCNNGA 2 cut(s) 166, 380
Hpy8I GTNNAC 2 cut(s) 89, 244
Hpy99I CGWCG 1 cut(s) 359
HpyAV CCTTC 4 cut(s) 76, 220, 283, 319
HpyCH4III ACNGT 3 cut(s) 123, 262, 505
HpyCH4IV ACGT 2 cut(s) 126, 246
HpyF10VI GCNNNNNNNGC 1 cut(s) 54
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 2 cut(s) 126, 246
HspAI GCGC 1 cut(s) 78
Kzo9I GATC 4 cut(s) 7, 231, 427, 456
LmnI GCTCC 1 cut(s) 38
LpnPI CCDG 6 cut(s) 58, 150, 224, 351, 393, 498
MaeII ACGT 2 cut(s) 126, 246
MaeIII GTNAC 1 cut(s) 266
MalI GATC 4 cut(s) 9, 233, 429, 458
MboI GATC 4 cut(s) 7, 231, 427, 456
MboII GAAGA 2 cut(s) 313, 353
MflI RGATCY 1 cut(s) 427
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 3 cut(s) 160, 181, 306
MnlI CCTC 3 cut(s) 21, 52, 502
MseI TTAA 1 cut(s) 201
MspI CCGG 1 cut(s) 45
MvnI CGCG 1 cut(s) 80
MwoI GCNNNNNNNGC 1 cut(s) 54
NdeII GATC 4 cut(s) 7, 231, 427, 456
NlaIV GGNNCC 1 cut(s) 40
NspV TTCGAA 1 cut(s) 465
PcsI WCGNNNNNNNCGW 1 cut(s) 471
PfeI GAWTC 3 cut(s) 177, 399, 462
Ple19I CGATCG 1 cut(s) 459
PspN4I GGNNCC 1 cut(s) 40
PsuI RGATCY 1 cut(s) 427
PvuI CGATCG 1 cut(s) 459
RsaI GTAC 2 cut(s) 125, 318
RsaNI GTAC 2 cut(s) 124, 317
SaqAI TTAA 1 cut(s) 201
Sau3AI GATC 4 cut(s) 7, 231, 427, 456
ScaI AGTACT 1 cut(s) 318
SduI GDGCHC 1 cut(s) 213
SetI ASST 9 cut(s) 101, 129, 221, 243, 249, 330, 472, 480, 519
SfuI TTCGAA 1 cut(s) 465
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 3 cut(s) 160, 181, 306
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 3 cut(s) 123, 262, 505
TaiI ACGT 2 cut(s) 129, 249
TaqI TCGA 4 cut(s) 455, 465, 474, 519
TasI AATT 3 cut(s) 160, 181, 306
TatI WGTACW 1 cut(s) 316
TfiI GAWTC 3 cut(s) 177, 399, 462
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TscAI CASTG 2 cut(s) 126, 267
TspDTI ATGAA 2 cut(s) 52, 294
TspRI CASTG 2 cut(s) 126, 267
XagI CCTNNNNNAGG 1 cut(s) 15
XapI RAATTY 1 cut(s) 160
ZrmI AGTACT 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.