Rw1G009700

isoflavone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0018227
Physical Location & Seq
Reverse (-)
21303376 .. 21303972
597 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G009700.1

Sequence Viewer

Length: 351 bp
ATGAATATTGCCCTGGGGTTTCATGCTGTGGATGACCCAAGAACATTGAACAAGATCCTCTACATCCGGCCACCTGCCAACACCTTCTCCTTCAACGAACTTGTGGCTCTTTGGGAGAAGAAGATTGGAAAAACCCTCGAGAAGGTCTATGTCCCAGAGGAGCAACTACTCAAAAACATTCAAGAGGCTGAGGTTCCAATCAATGTGATACTGGCAATCGGTCACTCAGTTTTTGTGAAAGGAGACCACACTAACTTTGAGATCGAGCCCTCATTTGGAGTGGAAGCTACTGCTCTTTACCATGATGTCAAATACACTACTGTGGATGAGTACCTCTCCCAGTTCGTCTGA

Protein Analysis

116

Amino Acids

13.19

Weight (kDa)

5.09

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 8 - 112 4.2e-29 NmrA-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 82
Acc36I ACCTGC 1 cut(s) 82
AclWI GGATC 1 cut(s) 49
AcoI YGGCCR 1 cut(s) 68
AfaI GTAC 1 cut(s) 332
AfiI CCNNNNNNNGG 2 cut(s) 142, 275
AgsI TTSAA 3 cut(s) 49, 94, 182
AjnI CCWGG 1 cut(s) 12
AleI CACNNNNGTG 1 cut(s) 320
AluBI AGCT 1 cut(s) 287
AluI AGCT 1 cut(s) 287
Alw26I GTCTC 1 cut(s) 237
AlwI GGATC 1 cut(s) 49
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 1 cut(s) 68
AvaI CYCGRG 1 cut(s) 137
BanII GRGCYC 1 cut(s) 270
BbvCI CCTCAGC 1 cut(s) 189
BciT130I CCWGG 1 cut(s) 14
BcoDI GTCTC 1 cut(s) 237
BfuAI ACCTGC 1 cut(s) 82
Bme1390I CCNGG 1 cut(s) 14
BmeT110I CYCGRG 1 cut(s) 137
BmiI GGNNCC 1 cut(s) 195
BmrFI CCNGG 1 cut(s) 14
BmrI ACTGGG 1 cut(s) 334
BmuI ACTGGG 1 cut(s) 334
BplI GAGNNNNNCTC 1 cut(s) 320
Bpu10I CCTNAGC 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 237
BsaJI CCNNGG 2 cut(s) 12, 13
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 275
Bse1I ACTGG 2 cut(s) 216, 340
BseBI CCWGG 1 cut(s) 14
BseDI CCNNGG 2 cut(s) 12, 13
BseGI GGATG 3 cut(s) 37, 63, 331
BseLI CCNNNNNNNGG 2 cut(s) 142, 275
BseMII CTCAG 2 cut(s) 180, 240
BseNI ACTGG 2 cut(s) 216, 340
BseRI GAGGAG 1 cut(s) 173
BshFI GGCC 1 cut(s) 70
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 67
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 2 cut(s) 142, 275
BsmAI GTCTC 1 cut(s) 237
BsmFI GGGAC 1 cut(s) 137
BsnI GGCC 1 cut(s) 70
Bso31I GGTCTC 1 cut(s) 237
BsoBI CYCGRG 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 270
Bsp143I GATC 2 cut(s) 54, 261
BspANI GGCC 1 cut(s) 70
BspCNI CTCAG 2 cut(s) 181, 239
BspLI GGNNCC 1 cut(s) 195
BspMI ACCTGC 1 cut(s) 82
BspPI GGATC 1 cut(s) 49
BspTNI GGTCTC 1 cut(s) 237
BsrI ACTGG 2 cut(s) 216, 340
BssECI CCNNGG 2 cut(s) 12, 13
BssMI GATC 2 cut(s) 54, 261
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 1 cut(s) 322
BstDEI CTNAG 2 cut(s) 189, 226
BstENI CCTNNNNNAGG 1 cut(s) 140
BstF5I GGATG 3 cut(s) 37, 63, 331
BstKTI GATC 2 cut(s) 57, 264
BstMAI GTCTC 1 cut(s) 237
BstMBI GATC 2 cut(s) 54, 261
BstNI CCWGG 1 cut(s) 14
BstSCI CCNGG 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 54
BstYI RGATCY 1 cut(s) 54
BsuRI GGCC 1 cut(s) 70
BtsCI GGATG 3 cut(s) 37, 63, 331
BveI ACCTGC 1 cut(s) 82
Csp6I GTAC 1 cut(s) 331
CviAII CATG 2 cut(s) 23, 302
CviJI RGCY 5 cut(s) 70, 107, 188, 268, 287
CviKI_1 RGCY 5 cut(s) 70, 107, 188, 268, 287
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 2 cut(s) 189, 226
DpnI GATC 2 cut(s) 56, 263
DpnII GATC 2 cut(s) 54, 261
EaeI YGGCCR 1 cut(s) 68
Eco24I GRGCYC 1 cut(s) 270
Eco31I GGTCTC 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 137
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 12
EcoT38I GRGCYC 1 cut(s) 270
FaeI CATG 2 cut(s) 26, 305
FaiI YATR 3 cut(s) 24, 150, 303
FaqI GGGAC 1 cut(s) 137
FatI CATG 2 cut(s) 22, 301
FokI GGATG 3 cut(s) 44, 50, 338
FriOI GRGCYC 1 cut(s) 270
HaeIII GGCC 1 cut(s) 70
HapII CCGG 1 cut(s) 67
Hin1II CATG 2 cut(s) 26, 305
HpaII CCGG 1 cut(s) 67
Hpy188I TCNGA 1 cut(s) 350
Hpy188III TCNNGA 2 cut(s) 139, 182
HpyAV CCTTC 3 cut(s) 94, 100, 136
HpyCH4III ACNGT 1 cut(s) 322
HpyF3I CTNAG 2 cut(s) 189, 226
Hsp92II CATG 2 cut(s) 26, 305
Kzo9I GATC 2 cut(s) 54, 261
LmnI GCTCC 1 cut(s) 160
LpnPI CCDG 5 cut(s) 26, 80, 87, 168, 197
MaeIII GTNAC 1 cut(s) 221
MalI GATC 2 cut(s) 56, 263
MboI GATC 2 cut(s) 54, 261
MboII GAAGA 2 cut(s) 130, 133
MflI RGATCY 1 cut(s) 54
MhlI GDGCHC 1 cut(s) 270
MnlI CCTC 7 cut(s) 68, 146, 151, 178, 184, 280, 344
MslI CAYNNNNRTG 1 cut(s) 320
MspI CCGG 1 cut(s) 67
MspR9I CCNGG 1 cut(s) 14
MvaI CCWGG 1 cut(s) 14
NdeII GATC 2 cut(s) 54, 261
NlaIII CATG 2 cut(s) 26, 305
NlaIV GGNNCC 1 cut(s) 195
NmuCI GTSAC 1 cut(s) 221
OliI CACNNNNGTG 1 cut(s) 320
PaeR7I CTCGAG 1 cut(s) 137
PaqCI CACCTGC 1 cut(s) 82
PasI CCCWGGG 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 12
PspGI CCWGG 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 195
PsuI RGATCY 1 cut(s) 54
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
RseI CAYNNNNRTG 1 cut(s) 320
Sau3AI GATC 2 cut(s) 54, 261
ScrFI CCNGG 1 cut(s) 14
SduI GDGCHC 1 cut(s) 270
SetI ASST 6 cut(s) 76, 86, 147, 195, 289, 336
Sfr274I CTCGAG 1 cut(s) 137
SlaI CTCGAG 1 cut(s) 137
SmiMI CAYNNNNRTG 1 cut(s) 320
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SspI AATATT 1 cut(s) 7
StyD4I CCNGG 1 cut(s) 12
TaaI ACNGT 1 cut(s) 322
TaqI TCGA 2 cut(s) 138, 264
TaqII GACCGA 1 cut(s) 209
TseFI GTSAC 1 cut(s) 221
Tsp45I GTSAC 1 cut(s) 221
TspDTI ATGAA 2 cut(s) 11, 17
XagI CCTNNNNNAGG 1 cut(s) 140
XhoI CTCGAG 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.