Rh4AG200300

isoflavone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0018227
Physical Location & Seq
Forward (+)
48551807 .. 48552776
970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG200300.1

Sequence Viewer

Length: 378 bp
ATGCAGCAATCTTTAACAAGGAAGAGGATATTGGTAGGTAATACCATTAAGGCTGTGGATGACCCAAGAACATTGAACAAGATCCTCTACATCCGGCCACCTGCCAACACCTTCTCCTTCAACGAACTTGTGGCTCTTTGGGAGAAGAAGATTGGAAAAACCCTCGAGAAGGTCTATGTCCCAGAGGAGCAACTACTCAAAAACATTCAAGAGGCTGAGGTTCCAATCAATGTGATACTGGCAATCGGTCACTCAGTTTTTGTGAAAGGAGACCACACTAACTTTGAGATCGAGCCCTCATTTGGAGTGGAAGCTACTGCTCTTTACCCTGATGTCAAATACACTACTGTGGATGAGTACCTCTCCCAGTTCGTCTGA

Protein Analysis

125

Amino Acids

14.24

Weight (kDa)

5.69

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 15 - 121 1.9e-30 NmrA-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 109
Acc36I ACCTGC 1 cut(s) 109
AclWI GGATC 1 cut(s) 76
AcoI YGGCCR 1 cut(s) 95
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 2 cut(s) 169, 302
AgsI TTSAA 3 cut(s) 76, 121, 209
AjuI GAANNNNNNNTTGG 2 cut(s) 14, 46
AleI CACNNNNGTG 1 cut(s) 347
AluBI AGCT 1 cut(s) 314
AluI AGCT 1 cut(s) 314
Alw26I GTCTC 1 cut(s) 264
AlwI GGATC 1 cut(s) 76
Ama87I CYCGRG 1 cut(s) 164
AoxI GGCC 1 cut(s) 95
ApeKI GCWGC 1 cut(s) 4
AvaI CYCGRG 1 cut(s) 164
BanII GRGCYC 1 cut(s) 297
BbvCI CCTCAGC 1 cut(s) 216
BbvI GCAGC 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 264
BfuAI ACCTGC 1 cut(s) 109
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
BmeT110I CYCGRG 1 cut(s) 164
BmiI GGNNCC 1 cut(s) 222
BmrI ACTGGG 1 cut(s) 361
BmuI ACTGGG 1 cut(s) 361
BplI GAGNNNNNCTC 1 cut(s) 347
Bpu10I CCTNAGC 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 264
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 2 cut(s) 169, 302
Bse1I ACTGG 2 cut(s) 243, 367
BseGI GGATG 3 cut(s) 64, 90, 358
BseLI CCNNNNNNNGG 2 cut(s) 169, 302
BseMII CTCAG 2 cut(s) 207, 267
BseNI ACTGG 2 cut(s) 243, 367
BseRI GAGGAG 1 cut(s) 200
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 1 cut(s) 97
BsiHKCI CYCGRG 1 cut(s) 164
BsiSI CCGG 1 cut(s) 94
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 2 cut(s) 169, 302
BsmAI GTCTC 1 cut(s) 264
BsmFI GGGAC 1 cut(s) 164
BsnI GGCC 1 cut(s) 97
Bso31I GGTCTC 1 cut(s) 264
BsoBI CYCGRG 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 2 cut(s) 81, 288
BspANI GGCC 1 cut(s) 97
BspCNI CTCAG 2 cut(s) 208, 266
BspLI GGNNCC 1 cut(s) 222
BspMI ACCTGC 1 cut(s) 109
BspPI GGATC 1 cut(s) 76
BspTNI GGTCTC 1 cut(s) 264
BsrI ACTGG 2 cut(s) 243, 367
BssMI GATC 2 cut(s) 81, 288
Bst4CI ACNGT 1 cut(s) 349
Bst6I CTCTTC 1 cut(s) 17
BstDEI CTNAG 2 cut(s) 216, 253
BstENI CCTNNNNNAGG 1 cut(s) 167
BstF5I GGATG 3 cut(s) 64, 90, 358
BstKTI GATC 2 cut(s) 84, 291
BstMAI GTCTC 1 cut(s) 264
BstMBI GATC 2 cut(s) 81, 288
BstV1I GCAGC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 81
BstYI RGATCY 1 cut(s) 81
BsuRI GGCC 1 cut(s) 97
BtsCI GGATG 3 cut(s) 64, 90, 358
BveI ACCTGC 1 cut(s) 109
Csp6I GTAC 1 cut(s) 358
CviJI RGCY 6 cut(s) 53, 97, 134, 215, 295, 314
CviKI_1 RGCY 6 cut(s) 53, 97, 134, 215, 295, 314
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 2 cut(s) 216, 253
DpnI GATC 2 cut(s) 83, 290
DpnII GATC 2 cut(s) 81, 288
EaeI YGGCCR 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco24I GRGCYC 1 cut(s) 297
Eco31I GGTCTC 1 cut(s) 264
Eco88I CYCGRG 1 cut(s) 164
EcoNI CCTNNNNNAGG 1 cut(s) 167
EcoT38I GRGCYC 1 cut(s) 297
FaiI YATR 1 cut(s) 177
FaqI GGGAC 1 cut(s) 164
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 3 cut(s) 71, 77, 365
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 1 cut(s) 5
GluI GCNGC 1 cut(s) 5
HaeIII GGCC 1 cut(s) 97
HapII CCGG 1 cut(s) 94
HpaII CCGG 1 cut(s) 94
Hpy188I TCNGA 1 cut(s) 377
Hpy188III TCNNGA 2 cut(s) 166, 209
HpyAV CCTTC 3 cut(s) 121, 127, 163
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4V TGCA 1 cut(s) 4
HpyF3I CTNAG 2 cut(s) 216, 253
Kzo9I GATC 2 cut(s) 81, 288
LmnI GCTCC 1 cut(s) 187
LpnPI CCDG 5 cut(s) 107, 114, 195, 224, 342
Lsp1109I GCAGC 1 cut(s) 16
MaeIII GTNAC 1 cut(s) 248
MalI GATC 2 cut(s) 83, 290
MboI GATC 2 cut(s) 81, 288
MboII GAAGA 3 cut(s) 34, 157, 160
MflI RGATCY 1 cut(s) 81
MhlI GDGCHC 1 cut(s) 297
MnlI CCTC 8 cut(s) 18, 95, 173, 178, 205, 211, 307, 371
MseI TTAA 2 cut(s) 14, 48
MslI CAYNNNNRTG 1 cut(s) 347
MspI CCGG 1 cut(s) 94
NdeII GATC 2 cut(s) 81, 288
NlaIV GGNNCC 1 cut(s) 222
NmuCI GTSAC 1 cut(s) 248
OliI CACNNNNGTG 1 cut(s) 347
PaeR7I CTCGAG 1 cut(s) 164
PaqCI CACCTGC 1 cut(s) 109
PkrI GCNGC 1 cut(s) 6
PspN4I GGNNCC 1 cut(s) 222
PsuI RGATCY 1 cut(s) 81
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
RseI CAYNNNNRTG 1 cut(s) 347
SaqAI TTAA 2 cut(s) 14, 48
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 2 cut(s) 81, 288
SduI GDGCHC 1 cut(s) 297
SetI ASST 7 cut(s) 40, 103, 113, 174, 222, 316, 363
Sfr274I CTCGAG 1 cut(s) 164
SlaI CTCGAG 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
TaaI ACNGT 1 cut(s) 349
TaqI TCGA 2 cut(s) 165, 291
TaqII GACCGA 1 cut(s) 236
Tru1I TTAA 2 cut(s) 14, 48
Tru9I TTAA 2 cut(s) 14, 48
TseFI GTSAC 1 cut(s) 248
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 248
XagI CCTNNNNNAGG 1 cut(s) 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XhoI CTCGAG 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.