Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0017445

Physical Location & Seq

Genomic Coordinates Forward (+)

10923552 .. 10933343

Gene Structure 9792 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000011603

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3807 bp

ATGGATCGCCTTTCCACCGTAAGATCCAAGTCCACTATTGACGACATCTCCGACCTCGAGTCCATTCAAATCAACAATGGCCGACCCCAGTTGATCAAACCTCAGCAACCATCCTCATCCTCCTCAGGCGTCCTCAACCAGATGCCCCACCTCTCCGACCACAAAGGCATCGCCGCCATGACTCCCGCCGTCTCTGACGTCACCCAGACCCACTCAGTCCTCAACGCCCTCGGCGACCGTCCAGACCACAAGGCCGTCGACACTGCCAAGAACTCCTCCCCCAACCACACCCTCAACGGCGATCCAAACTCTCCTTCATCACCCCCTCCGAAATCACAGCCGAATTCTCCCACCACGACCCCAACGTTGCCCGGATCAACAATGGCAGCTTCGAGTCCTGCCCCGACTCCATCATCCAGCCTCCTAGAGTCCAAGTGGGAGAATTGGGGTGGGATTGTGAGCAAGCTATGGCCAACTGCAGCCTATTTTGAGTTTCTTCCATTTGATATGAATGGGGGTGAGGCTATTGGTAGAGAAGTAGTTGATGTTTTAGGTGTTGAAGTGGGGAAGTCTTGGAACTCTCAACTTCGGAGGTTCCAGACTCTCTACCTTGGGCATTGTGGGATGTCTAATCTCAGTGAAGATCTTGTGTTCTTGGTCTTAAAGTATTTGGATGAGGAAGGTTATAAGGAAGCCGCCCACATGCTTGAGCGTGAAAGTGGGCTTTACTTTGACATGAATTATTTTGAAGAGCTGGTGCTTAGTGGAAAATGGGATGAGGCAGAGAAATACCTCGCCGGCTTCACAAAGCTTGATGACAATAAGTACTCGACTAAGATCTACTTTGAAATCAGGAAGCAACATTACTTTGAGGCACTTGATAAAAAATGTCGTGCCGAGGCTTTAGATATCCTCACAAATGATCTACAAGTTTTTGCTAAAGGTAATGAAGAGCTATTCAAAGAAATGACTCAACTTATGACATTTGAGAATATAAGGGAACATGAGTCACTCTCCATGTATAGAGATACAAAGTCTGCAAGGAAGGTTATGATGAAAGAAATCAGGAGGGTAATTGAGGCAAATCCAATTTTCCGTGGTAGACTGGAATTTCCTATCATAAAAGGCGAGAGATTACGGCGTCTGATTAACCAAAGCTTGAATTGGCAGCATATGCAATGTGAAGATCCTCATCCAAATCCAGCCATTGACACCCTTTTTATTGATCATGTTTGTGACCCACAGGTCTGTATACAACATTCTCCATCAATTGAGAATAATGCAAAACCTTCCCAGACCACATCATATCAATCTCCAACTTGTTCTGTGAAGTCAAGTCTATCTACCATTGCACAATATGCATCGTGTATTGGAGGATCTTTCCCTGCAAAGAACCTGGCTACTGAGGTTATTCAGGATTCTGATGTTTTATCCAAAAGGATGCCCATTGTAACTGGGGATGAGGTAACATCAACTATCACTGATCATGGCCAAAATCAGATCTCGGAACTTGCCATATCCAAAAATCTTCCCAGTACTGTTGTCCGTACATTGAATGAGGGGTCTTCTCCAGTGAGCATGGATTTTCATCCCATTTGGCTTACTGTTCTTTTAGTTGGAACTTCTGCTGGGGAAATAGGACTCTGGGATACTAGCTGCGGGGGAAAAATGGTTGCCAGAAACTTTAATGTTTGGGATCTTGGAGCATGCTCAATGGCCTTAAGGCTAGCACTAGAAAAAGATTCACATGTCTCTGTTAATCGTGTGTTATGGAGTCCGGATGGTTTCTTATTTGGGGTTGCATATTCAAAACATGTTGTGCAATTATATTCTTATATTGGAGGAGATGAACTGGATCAAATATTGGAAATTGATGCTCATTTGGGTGGTGTAAATGATTTAGCATTCTCTTCTCCTGAAGGGGAACTTTTGGTCATAACATGTGGTGATGACAGGACCATCAAGGTGTGGGATGTGGTTAGTGGTAACCGATGCTTTACTTTTGAAGGTCATGATGCTCCTGTTTATTCCATCTGTCCTCATATCAAAGACAATATCCCTTTTATCTTTTCAGTCTCAACAGTTGGCAGGATCAAAGTGTGGTTATATGACAATTTAGGAGACAGAATTGATTATGATGCTCCTGGTCTTGGTTGCACCAAAATGGCTTACAGTGCTGATGAGAGGCTTTTTTCATGTGGGACCAGTAAAGGAGGGGAATCATATCTTGTTGAATGGGATGATACTGTTGGTTGTTTCAAAAGAGTTTACAAAGGACTTGGGCCAAACCCCTTGTCTGCCGTGCAATTCGATATAACCAGGAACCAGCTTTTGGCTGCTGGTGATGAACATATGGTTAAATTTTGGCATATGGACAACACTCAACTTTTGAAAACCATTGATGCTGATGGTGGATTGCCAGCAAATCCACATATTCGCTTCAACAGTGATGGCACACTATTAGCTGTTTCTTCTAGTGGAAACCGAATCAAGATACTGGCAAATGCAAATGGTCTTGATTTGCTGCAAGGATGTGGGAATGATTCTATCGTTGCCTCTGGAGTTGAATCTGAAACTTCAAGAAAGAATGGGAATGTGGCAGAAGTGAATTCAAAAACAACAGGAGAAGCCACTTACGGAGAAAAGGCATGGGAAACTATTGAGAGACCTTCTCAATGCCAATCTTTGCAGCTATGTGCTGATGTCAAAAGAACCAAGATATCCAAGCTGATCTACACCAATGCCGGTGACGCCATTTTGGCATTAACATCAAATGCCATTCATCTACTCTGGACATGGCCGCAGAACGATCTTAGTTCTAGTGGAAAGGCAACTACCAAGGTTCAACCTCTTTTATGGCGACCAACGAGTGGCTTACAGCTGATGAGCAATGACCTTTCCAGCACCAACCCTGAAGAGGCCATGCCATGCTTTGCTTTGTCCAAGAATGATTCATATCTTATGTCAGCATCAGGAGGGATGATCTCCCTTTTTAATGTTATATCGTTTAAGACAATGAGGAAAGTCATGGCTCCACCACCTGCAGCAACATGCCTTGCTTTTGATCCTCGAGACAACAATGTAGTTGCTATTGGCATGGATGATTCCACAATTATGATATATAATGCTCGCTTGGATAAGGTCACAAATATGCTTGCTGGTCACACCAAAAGAGTCACTTCCCTTGCCTTCTCTACCTCTATGAAATCACTTGTTTCTTCTGGTGCTGATGCTCAGATTTTTGTGTGGAATGCTGATGAGTGGAAAATACAGAGAAGCCAAGTGCTGCAGATTCCAGTTTGGAACAAATTGAGAGCATTATCAGACTCAGATACCCATGTCCGGTTTCACGAGGATCAGCTGCATTTCCTAATCGTACACAAGACTCACCTTGCAATATACGAAGTGAAAGAACTAGGATGTGTAAACGAGTGGGTCTCAGATTCTTCTGTACCAATTTCCCATGCTACGTTCTCATGTGATAGCCAGACAGTATATGCCAGCTTTGTGGATGGAACCGTAGGCATATTTGACGCTTCTGTTCTTGTACTATGCTGTCTAATCAAGCCCTCCGCTTACCTTCCTACCGATATAAGTTGCAGTTATACTGTGTACCCAGTCGTCATTGCGGGGCATCCACAAAAACCAACCCAGTTTGCCGTTGGACTCTCTAATGGTGAAGTTCATGTGCTTGAGCCTTTAGAATCAGAAGGCAAATGGGGTATATTGCCAACGGTTAAAAGGAATTTGGAGAGCAGTGCGCCTGCAAAATCTACTTTTCGGGGCTTCTTATCGACCACCTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010051 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048523 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0090421 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1269

Amino Acids

139.59

Weight (kDa)

5.53

Isoelectric Point (pI)

39.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	213 - 241	6.2e-06	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	246 - 369	1.1e-53	TPR1-like, CTLH-containing domain
zf_topless	PF21359	372 - 412	1e-21	TOPLESS, zinc finger domain
Beta-prop_WDR3_1st	PF25173	561 - 703	4.5e-14	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	563 - 678	2e-11	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	584 - 702	5.5e-13	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	599 - 704	2.5e-08	WDHD1 first WD40 domain
WD40	PF00400	621 - 658	9.2e-07	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	721 - 838	3e-06	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	964 - 1180	2.6e-16	WDHD1 first WD40 domain
WDR55	PF24796	965 - 1085	6.2e-07	WDR55
Beta-prop_EML_2	PF23414	968 - 1102	5.8e-10	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	969 - 1089	5.9e-11	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	973 - 1179	2.2e-19	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	977 - 1124	2.1e-13	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	981 - 1089	7.4e-12	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	988 - 1085	2.2e-14	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	988 - 1090	1.8e-09	WDR36/Utp21 first beta-propeller
Beta-prop_IFT140_1st	PF23383	989 - 1102	1.6e-08	IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	991 - 1085	1.5e-08	WDR36/Utp21 second beta-propeller domain
WD40_Prp19	PF24814	1013 - 1179	4.3e-15	Prp19 WD40 domain
Beta-prop_WDR19_1st	PF23389	1017 - 1138	5.7e-07	WDR19 first beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	1020 - 1084	1.2e-06	MABP1/WDR62 second WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017445)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr6g0297391
rosa_laevigata	RLG00000011603
rosa_multiflora	Rmu_sc0004989.1_g000003
rosa_roxburghii	Rroxscaffold_7G00170560
rosa_rugosa	Rorug06G0271300 Rorug06G0271400 Rorug06G0271500 Rorug06G0271600
rosa_samantha	Rh6AG382800 Rh6BG391100 Rh6CG396000 Rh6DG383300
rosa_wichuraiana	Rw6G033340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	687
AarI	CACCTGC	1 cut(s)	3049
AasI	GACNNNNNNGTC	1 cut(s)	1244
AatII	GACGTC	1 cut(s)	201
Acc36I	ACCTGC	1 cut(s)	3049
AccB7I	CCANNNNNTGG	2 cut(s)	2332, 2870
AccI	GTMKAC	3 cut(s)	258, 1102, 1252
AccIII	TCCGGA	1 cut(s)	1777
AciI	CCGC	7 cut(s)	174, 186, 696, 1659, 2801, 3573, 3629
AclI	AACGTT	1 cut(s)	365
AcoI	YGGCCR	4 cut(s)	79, 470, 1489, 2798
AcsI	RAATTY	5 cut(s)	343, 1109, 2360, 2608, 3745
AcuI	CTGAAG	2 cut(s)	1938, 2934
AcyI	GRCGYC	4 cut(s)	129, 198, 1141, 2751
AfaI	GTAC	7 cut(s)	827, 1537, 1549, 3378, 3453, 3549, 3614
AfiI	CCNNNNNNNGG	5 cut(s)	2150, 2332, 2870, 2917, 3342
AflII	CTTAAG	1 cut(s)	1720
AflIII	ACRYGT	3 cut(s)	1747, 1813, 1940
AhdI	GACNNNNNGTC	1 cut(s)	58
AjnI	CCWGG	3 cut(s)	1395, 2143, 2318
AjuI	GAANNNNNNNTTGG	4 cut(s)	298, 330, 2315, 2347
Alw26I	GTCTC	7 cut(s)	196, 1756, 2080, 2115, 2659, 3066, 3442
Ama87I	CYCGRG	2 cut(s)	56, 3069
Aor13HI	TCCGGA	1 cut(s)	1777
AoxI	GGCC	8 cut(s)	79, 252, 470, 1489, 1716, 2282, 2798, 2919
ApoI	RAATTY	5 cut(s)	343, 1109, 2360, 2608, 3745
ArsI	GACNNNNNNTTYG	2 cut(s)	2279, 2311
AspLEI	GCGC	1 cut(s)	3763
AspS9I	GGNCC	3 cut(s)	1956, 2202, 2282
AsuC2I	CCSGG	1 cut(s)	372
AsuHPI	GGTGA	8 cut(s)	193, 312, 530, 1958, 2354, 2759, 3380, 3689
AsuNHI	GCTAGC	1 cut(s)	1726
AvaI	CYCGRG	2 cut(s)	56, 3069
AvaII	GGWCC	2 cut(s)	1956, 2202
AxyI	CCTNAGG	1 cut(s)	124
BaeI	ACNNNNGTAYC	2 cut(s)	3324, 3357
BalI	TGGCCA	2 cut(s)	472, 1491
BarI	GAAGNNNNNNTAC	2 cut(s)	848, 880
BauI	CACGAG	1 cut(s)	3350
BbsI	GAAGAC	1 cut(s)	1557
BbvCI	CCTCAGC	1 cut(s)	102
BccI	CCATC	9 cut(s)	118, 418, 1273, 1775, 1967, 2039, 2402, 2444, 3506
BceAI	ACGGC	6 cut(s)	173, 239, 313, 1154, 2285, 3644
BciT130I	CCWGG	3 cut(s)	1397, 2145, 2320
BciVI	GTATCC	1 cut(s)	1642
BclI	TGATCA	3 cut(s)	93, 1225, 1483
BcnI	CCSGG	1 cut(s)	372
BcoDI	GTCTC	7 cut(s)	196, 1756, 2080, 2115, 2659, 3066, 3442
BfaI	CTAG	7 cut(s)	425, 1653, 1727, 1733, 2475, 2820, 3416
BfmI	CTRYAG	3 cut(s)	477, 3042, 3287
BfrI	CTTAAG	1 cut(s)	1720
BfuAI	ACCTGC	1 cut(s)	3049
BfuI	GTATCC	1 cut(s)	1642
BglI	GCCNNNNNGGC	1 cut(s)	2759
BglII	AGATCT	3 cut(s)	643, 837, 1500
BmcAI	AGTACT	2 cut(s)	827, 1537
Bme1390I	CCNGG	4 cut(s)	372, 1397, 2145, 2320
Bme18I	GGWCC	2 cut(s)	1956, 2202
BmeRI	GACNNNNNGTC	1 cut(s)	58
BmeT110I	CYCGRG	2 cut(s)	56, 3069
BmgT120I	GGNCC	3 cut(s)	1956, 2202, 2282
BmiI	GGNNCC	5 cut(s)	596, 2203, 2324, 3033, 3517
BmrFI	CCNGG	4 cut(s)	372, 1397, 2145, 2320
BmrI	ACTGGG	5 cut(s)	82, 1464, 1527, 3611, 3646
BmtI	GCTAGC	1 cut(s)	1730
BmuI	ACTGGG	5 cut(s)	82, 1464, 1527, 3611, 3646
BpiI	GAAGAC	1 cut(s)	1557
BplI	GAGNNNNNCTC	6 cut(s)	998, 1030, 2656, 2688, 3422, 3454
BpmI	CTGGAG	2 cut(s)	1554, 2580
Bpu10I	CCTNAGC	1 cut(s)	102
BpuEI	CTTGAG	2 cut(s)	728, 3713
BpuMI	CCSGG	1 cut(s)	372
BsaBI	GATNNNNATC	3 cut(s)	1191, 1587, 2955
BsaHI	GRCGYC	4 cut(s)	129, 198, 1141, 2751
BsaI	GGTCTC	2 cut(s)	2659, 3442
BsaJI	CCNNGG	5 cut(s)	229, 610, 897, 1096, 2838
BsaWI	WCCGGW	2 cut(s)	1777, 3342
BsaXI	ACNNNNNCTCC	2 cut(s)	32, 62
Bsc4I	CCNNNNNNNGG	5 cut(s)	2150, 2332, 2870, 2917, 3342
Bse118I	RCCGGY	2 cut(s)	797, 2744
Bse21I	CCTNAGG	1 cut(s)	124
Bse3DI	GCAATG	4 cut(s)	1184, 1347, 2896, 3624
Bse8I	GATNNNNATC	3 cut(s)	1191, 1587, 2955
BseAI	TCCGGA	1 cut(s)	1777
BseBI	CCWGG	3 cut(s)	1397, 2145, 2320
BseDI	CCNNGG	5 cut(s)	229, 610, 897, 1096, 2838
BseJI	GATNNNNATC	3 cut(s)	1191, 1587, 2955
BseLI	CCNNNNNNNGG	5 cut(s)	2150, 2332, 2870, 2917, 3342
BseMI	GCAATG	4 cut(s)	1184, 1347, 2896, 3624
BseMII	CTCAG	8 cut(s)	116, 138, 228, 649, 1395, 3248, 3342, 3453
BseRI	GAGGAG	3 cut(s)	112, 265, 1857
BseYI	CCCAGC	1 cut(s)	1628
Bsh1285I	CGRYCG	1 cut(s)	238
BshFI	GGCC	8 cut(s)	81, 254, 472, 1491, 1718, 2284, 2800, 2921
BsiEI	CGRYCG	1 cut(s)	238
BsiHKCI	CYCGRG	2 cut(s)	56, 3069
BsiSI	CCGG	5 cut(s)	372, 798, 1778, 2745, 3343
BslFI	GGGAC	1 cut(s)	2215
BslI	CCNNNNNNNGG	5 cut(s)	2150, 2332, 2870, 2917, 3342
BsmAI	GTCTC	7 cut(s)	196, 1756, 2080, 2115, 2659, 3066, 3442
BsmBI	CGTCTC	1 cut(s)	196
BsmFI	GGGAC	1 cut(s)	2215
BsmI	GAATGC	2 cut(s)	1904, 3256
BsnI	GGCC	8 cut(s)	81, 254, 472, 1491, 1718, 2284, 2800, 2921
Bso31I	GGTCTC	2 cut(s)	2659, 3442
BsoBI	CYCGRG	2 cut(s)	56, 3069
Bsp13I	TCCGGA	1 cut(s)	1777
BspACI	CCGC	7 cut(s)	174, 186, 696, 1659, 2801, 3573, 3629
BspANI	GGCC	8 cut(s)	81, 254, 472, 1491, 1718, 2284, 2800, 2921
BspCNI	CTCAG	8 cut(s)	115, 137, 227, 648, 1396, 3247, 3341, 3452
BspEI	TCCGGA	1 cut(s)	1777
BspHI	TCATGA	1 cut(s)	2011
BspLI	GGNNCC	5 cut(s)	596, 2203, 2324, 3033, 3517
BspMAI	CTGCAG	3 cut(s)	481, 3046, 3291
BspMI	ACCTGC	1 cut(s)	3049
BspOI	GCTAGC	1 cut(s)	1730
BspQI	GCTCTTC	2 cut(s)	744, 945
BspTI	CTTAAG	1 cut(s)	1720
BspTNI	GGTCTC	2 cut(s)	2659, 3442
BsrDI	GCAATG	4 cut(s)	1184, 1347, 2896, 3624
BsrFI	RCCGGY	2 cut(s)	797, 2744
BssAI	RCCGGY	2 cut(s)	797, 2744
BssECI	CCNNGG	5 cut(s)	229, 610, 897, 1096, 2838
BssNAI	GTATAC	1 cut(s)	1253
BssNI	GRCGYC	4 cut(s)	129, 198, 1141, 2751
BssSI	CACGAG	1 cut(s)	3350
BssT1I	CCWWGG	2 cut(s)	610, 2838
Bst1107I	GTATAC	1 cut(s)	1253
Bst2BI	CACGAG	1 cut(s)	3350
Bst2UI	CCWGG	3 cut(s)	1397, 2145, 2320
Bst6I	CTCTTC	4 cut(s)	744, 945, 1915, 2910
BstACI	GRCGYC	4 cut(s)	129, 198, 1141, 2751
BstAFI	CTTAAG	1 cut(s)	1720
BstAPI	GCANNNNNTGC	2 cut(s)	1174, 1358
BstC8I	GCNNGC	9 cut(s)	464, 799, 1708, 1728, 2421, 3130, 3156, 3502, 3765
BstDSI	CCRYGG	1 cut(s)	1096
BstEII	GGTNACC	1 cut(s)	1985
BstHHI	GCGC	1 cut(s)	3763
BstMAI	GTCTC	7 cut(s)	196, 1756, 2080, 2115, 2659, 3066, 3442
BstMCI	CGRYCG	1 cut(s)	238
BstMWI	GCNNNNNNNGC	5 cut(s)	1174, 1358, 2174, 2750, 2759
BstNI	CCWGG	3 cut(s)	1397, 2145, 2320
BstNSI	RCATGY	6 cut(s)	706, 1710, 1751, 1817, 1944, 3054
BstPI	GGTNACC	1 cut(s)	1985
BstSCI	CCNGG	4 cut(s)	370, 1395, 2143, 2318
BstSFI	CTRYAG	3 cut(s)	477, 3042, 3287
BstV2I	GAAGAC	1 cut(s)	1557
BstX2I	RGATCY	7 cut(s)	23, 643, 837, 1186, 1376, 1500, 1696
BstXI	CCANNNNNNTGG	1 cut(s)	3508
BstYI	RGATCY	7 cut(s)	23, 643, 837, 1186, 1376, 1500, 1696
BstZ17I	GTATAC	1 cut(s)	1253
Bsu36I	CCTNAGG	1 cut(s)	124
BsuI	GTATCC	1 cut(s)	1642
BsuRI	GGCC	8 cut(s)	81, 254, 472, 1491, 1718, 2284, 2800, 2921
BtgI	CCRYGG	1 cut(s)	1096
BtgZI	GCGATG	1 cut(s)	154
BtsI	GCAGTG	2 cut(s)	261, 3763
BtsIMutI	CAGTG	7 cut(s)	261, 643, 1479, 1578, 2179, 2452, 3763
BveI	ACCTGC	1 cut(s)	3049
Cac8I	GCNNGC	9 cut(s)	464, 799, 1708, 1728, 2421, 3130, 3156, 3502, 3765
CciI	TCATGA	1 cut(s)	2011
CfoI	GCGC	1 cut(s)	3763
Cfr10I	RCCGGY	2 cut(s)	797, 2744
Cfr13I	GGNCC	3 cut(s)	1956, 2202, 2282
CseI	GACGC	4 cut(s)	118, 1130, 2759, 3542
Csp6I	GTAC	7 cut(s)	826, 1536, 1548, 3377, 3452, 3548, 3613
CviQI	GTAC	7 cut(s)	826, 1536, 1548, 3377, 3452, 3548, 3613
DrdI	GACNNNNNNGTC	1 cut(s)	1244
DriI	GACNNNNNGTC	1 cut(s)	58
DseDI	GACNNNNNNGTC	1 cut(s)	1244
EaeI	YGGCCR	4 cut(s)	79, 470, 1489, 2798
Eam1104I	CTCTTC	4 cut(s)	744, 945, 1915, 2910
Eam1105I	GACNNNNNGTC	1 cut(s)	58
EarI	CTCTTC	4 cut(s)	744, 945, 1915, 2910
Eco130I	CCWWGG	2 cut(s)	610, 2838
Eco31I	GGTCTC	2 cut(s)	2659, 3442
Eco32I	GATATC	2 cut(s)	910, 2721
Eco47I	GGWCC	2 cut(s)	1956, 2202
Eco57I	CTGAAG	2 cut(s)	1938, 2934
Eco81I	CCTNAGG	1 cut(s)	124
Eco88I	CYCGRG	2 cut(s)	56, 3069
Eco91I	GGTNACC	1 cut(s)	1985
EcoO65I	GGTNACC	1 cut(s)	1985
EcoRI	GAATTC	2 cut(s)	343, 2608
EcoRII	CCWGG	3 cut(s)	1395, 2143, 2318
EcoRV	GATATC	2 cut(s)	910, 2721
EcoT14I	CCWWGG	2 cut(s)	610, 2838
EcoT22I	ATGCAT	1 cut(s)	1363
ErhI	CCWWGG	2 cut(s)	610, 2838
Esp3I	CGTCTC	1 cut(s)	196
FalI	AAGNNNNNCTT	6 cut(s)	827, 859, 1911, 1943, 3163, 3195
FaqI	GGGAC	1 cut(s)	2215
FauI	CCCGC	3 cut(s)	193, 1652, 3622
FauNDI	CATATG	3 cut(s)	1173, 2352, 2370
FbaI	TGATCA	3 cut(s)	93, 1225, 1483
FblI	GTMKAC	3 cut(s)	258, 1102, 1252
FspBI	CTAG	7 cut(s)	425, 1653, 1727, 1733, 2475, 2820, 3416
GlaI	GCGC	1 cut(s)	3762
GsaI	CCCAGC	1 cut(s)	1632
GsuI	CTGGAG	2 cut(s)	1554, 2580
HaeIII	GGCC	8 cut(s)	81, 254, 472, 1491, 1718, 2284, 2800, 2921
HapII	CCGG	5 cut(s)	372, 798, 1778, 2745, 3343
HgaI	GACGC	4 cut(s)	118, 1130, 2759, 3542
HhaI	GCGC	1 cut(s)	3763
Hin1I	GRCGYC	4 cut(s)	129, 198, 1141, 2751
Hin6I	GCGC	1 cut(s)	3761
HinP1I	GCGC	1 cut(s)	3761
HincII	GTYRAC	1 cut(s)	259
HindII	GTYRAC	1 cut(s)	259
HindIII	AAGCTT	2 cut(s)	809, 1156
HpaII	CCGG	5 cut(s)	372, 798, 1778, 2745, 3343
HphI	GGTGA	8 cut(s)	193, 312, 530, 1958, 2354, 2759, 3380, 3689
Hpy166II	GTNNAC	8 cut(s)	33, 259, 1103, 1253, 2269, 3379, 3427, 3613
Hpy8I	GTNNAC	8 cut(s)	33, 259, 1103, 1253, 2269, 3379, 3427, 3613
Hpy99I	CGWCG	1 cut(s)	260
HpyCH4IV	ACGT	3 cut(s)	198, 365, 3470
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1174, 1358, 2174, 2750, 2759
HpySE526I	ACGT	3 cut(s)	198, 365, 3470
Hsp92I	GRCGYC	4 cut(s)	129, 198, 1141, 2751
HspAI	GCGC	1 cut(s)	3761
Kpn2I	TCCGGA	1 cut(s)	1777
KroI	GCCGGC	1 cut(s)	797
KroNI	GCCGGC	1 cut(s)	799
Ksp22I	TGATCA	3 cut(s)	93, 1225, 1483
LguI	GCTCTTC	2 cut(s)	744, 945
LmnI	GCTCC	4 cut(s)	1703, 2023, 2146, 3037
MaeI	CTAG	7 cut(s)	425, 1653, 1727, 1733, 2475, 2820, 3416
MaeII	ACGT	3 cut(s)	198, 365, 3470
MfeI	CAATTG	1 cut(s)	1269
MflI	RGATCY	7 cut(s)	23, 643, 837, 1186, 1376, 1500, 1696
MlsI	TGGCCA	2 cut(s)	472, 1491
MluNI	TGGCCA	2 cut(s)	472, 1491
MmeI	TCCRAC	5 cut(s)	75, 180, 1340, 1597, 3643
Mox20I	TGGCCA	2 cut(s)	472, 1491
Mph1103I	ATGCAT	1 cut(s)	1363
MroI	TCCGGA	1 cut(s)	1777
MroNI	GCCGGC	1 cut(s)	797
MscI	TGGCCA	2 cut(s)	472, 1491
MslI	CAYNNNNRTG	6 cut(s)	1233, 1884, 1964, 2162, 3113, 3149
Msp20I	TGGCCA	2 cut(s)	472, 1491
MspA1I	CMGCKG	2 cut(s)	2881, 3361
MspCI	CTTAAG	1 cut(s)	1720
MspI	CCGG	5 cut(s)	372, 798, 1778, 2745, 3343
MspR9I	CCNGG	4 cut(s)	372, 1397, 2145, 2320
MunI	CAATTG	1 cut(s)	1269
Mva1269I	GAATGC	2 cut(s)	1904, 3256
MvaI	CCWGG	3 cut(s)	1397, 2145, 2320
MwoI	GCNNNNNNNGC	5 cut(s)	1174, 1358, 2174, 2750, 2759
NaeI	GCCGGC	1 cut(s)	799
NciI	CCSGG	1 cut(s)	372
NdeI	CATATG	3 cut(s)	1173, 2352, 2370
NgoMIV	GCCGGC	1 cut(s)	797
NheI	GCTAGC	1 cut(s)	1726
NlaIV	GGNNCC	5 cut(s)	596, 2203, 2324, 3033, 3517
NmeAIII	GCCGAG	2 cut(s)	210, 922
NmuCI	GTSAC	7 cut(s)	199, 1008, 1235, 2747, 3142, 3161, 3175
NsiI	ATGCAT	1 cut(s)	1363
NspI	RCATGY	6 cut(s)	706, 1710, 1751, 1817, 1944, 3054
PaeI	GCATGC	1 cut(s)	1710
PaeR7I	CTCGAG	2 cut(s)	56, 3069
PagI	TCATGA	1 cut(s)	2011
PaqCI	CACCTGC	1 cut(s)	3049
PciI	ACATGT	3 cut(s)	1747, 1813, 1940
PciSI	GCTCTTC	2 cut(s)	744, 945
PcsI	WCGNNNNNNNCGW	3 cut(s)	48, 231, 362
PctI	GAATGC	2 cut(s)	1904, 3256
PdiI	GCCGGC	1 cut(s)	799
PflMI	CCANNNNNTGG	2 cut(s)	2332, 2870
PscI	ACATGT	3 cut(s)	1747, 1813, 1940
PsiI	TTATAA	1 cut(s)	687
Psp1406I	AACGTT	1 cut(s)	365
Psp6I	CCWGG	3 cut(s)	1395, 2143, 2318
PspEI	GGTNACC	1 cut(s)	1985
PspFI	CCCAGC	1 cut(s)	1628
PspGI	CCWGG	3 cut(s)	1395, 2143, 2318
PspN4I	GGNNCC	5 cut(s)	596, 2203, 2324, 3033, 3517
PspPI	GGNCC	3 cut(s)	1956, 2202, 2282
PspXI	VCTCGAGB	1 cut(s)	56
PstI	CTGCAG	3 cut(s)	481, 3046, 3291
PsuI	RGATCY	7 cut(s)	23, 643, 837, 1186, 1376, 1500, 1696
PvuII	CAGCTG	2 cut(s)	2881, 3361
RsaI	GTAC	7 cut(s)	827, 1537, 1549, 3378, 3453, 3549, 3614
RsaNI	GTAC	7 cut(s)	826, 1536, 1548, 3377, 3452, 3548, 3613
RseI	CAYNNNNRTG	6 cut(s)	1233, 1884, 1964, 2162, 3113, 3149
SalI	GTCGAC	1 cut(s)	257
SapI	GCTCTTC	2 cut(s)	744, 945
Sau96I	GGNCC	3 cut(s)	1956, 2202, 2282
ScaI	AGTACT	2 cut(s)	827, 1537
ScrFI	CCNGG	4 cut(s)	372, 1397, 2145, 2320
SfcI	CTRYAG	3 cut(s)	477, 3042, 3287
Sfr274I	CTCGAG	2 cut(s)	56, 3069
SinI	GGWCC	2 cut(s)	1956, 2202
SlaI	CTCGAG	2 cut(s)	56, 3069
SmiMI	CAYNNNNRTG	6 cut(s)	1233, 1884, 1964, 2162, 3113, 3149
SmlI	CTYRAG	5 cut(s)	56, 707, 1720, 3069, 3692
SmoI	CTYRAG	5 cut(s)	56, 707, 1720, 3069, 3692
SphI	GCATGC	1 cut(s)	1710
SsiI	CCGC	7 cut(s)	174, 186, 696, 1659, 2801, 3573, 3629
SspI	AATATT	1 cut(s)	1863
SspMI	CTAG	7 cut(s)	425, 1653, 1727, 1733, 2475, 2820, 3416
StyD4I	CCNGG	4 cut(s)	370, 1395, 2143, 2318
StyI	CCWWGG	2 cut(s)	610, 2838
TaiI	ACGT	3 cut(s)	201, 368, 3473
TaqI	TCGA	7 cut(s)	57, 258, 392, 830, 2310, 3070, 3794
TatI	WGTACW	3 cut(s)	825, 1535, 3547
TauI	GCSGC	3 cut(s)	176, 698, 2803
TscAI	CASTG	7 cut(s)	268, 643, 1486, 1578, 2179, 2452, 3763
TseFI	GTSAC	7 cut(s)	199, 1008, 1235, 2747, 3142, 3161, 3175
Tsp45I	GTSAC	7 cut(s)	199, 1008, 1235, 2747, 3142, 3161, 3175
TspGWI	ACGGA	3 cut(s)	1085, 1535, 2652
TspRI	CASTG	7 cut(s)	268, 643, 1486, 1578, 2179, 2452, 3763
Van91I	CCANNNNNTGG	2 cut(s)	2332, 2870
Vha464I	CTTAAG	1 cut(s)	1720
VpaK11BI	GGWCC	2 cut(s)	1956, 2202
XapI	RAATTY	5 cut(s)	343, 1109, 2360, 2608, 3745
XceI	RCATGY	6 cut(s)	706, 1710, 1751, 1817, 1944, 3054
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3659
XhoI	CTCGAG	2 cut(s)	56, 3069
XmiI	GTMKAC	3 cut(s)	258, 1102, 1252
XspI	CTAG	7 cut(s)	425, 1653, 1727, 1733, 2475, 2820, 3416
ZraI	GACGTC	1 cut(s)	199
ZrmI	AGTACT	2 cut(s)	827, 1537
Zsp2I	ATGCAT	1 cut(s)	1363

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.