Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0017445

Physical Location & Seq

Genomic Coordinates Reverse (-)

58171563 .. 58179904

Gene Structure 8342 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG382800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3270 bp

ATGAATGGGGGTGAGGCTACTGGTAGAGAAGTAGTTGATGTTTCAGGTGTTGGAGTGGGGAAGGCCATTCGCACCCCAAGTGGAGCTAAAATCAGAGACACAATCACAATTGTTCCTCTGCTCTTCAGTCTTGAAACTCTCAACTTGAGGGGTTCCAGACTCTCTACCTTGGGCATTGTGGGATATGAAGAAGGTTATAAGGAAGCCGCCCACATGCTTGAGCGTGAAAGTGGGCTTTACTTTGACATGAATTATTTTGAAGAGCTGGTGCTTAGTGGAAAATGGGATGAAGCAGAGAAATACCTCGCCGGCTTCACAAAGCTCGATGACAATAAGTACTCGACTAAGATCTACTTTGAAATCAGGAAGCAACATTACTTTGAGGCACTTGACAAAAAATGTCGTGCCGAGGCTTTAGATGTCCTCACAAATGATCTACAAGTTTTTGCTAAAGGTAATGAAGAGCTATTCAAAGAAATGACTCAACTTATGACATTTGAGAATATAAGGGAACATGAGTCACTCTCTATGTATAGAGATACAAAGTCTGCAAGGAAGGTTATGATGAAAGAAATCAGGAGGGTAATTGAGGCAAATCCAATTTTCCGTGGTAGACTGGAATTTCCTATCGTAAAAGGCGAGAGATTACGGCGTCTGATTAACCAAAGCTTGAATTGGCAGCATATGCAATGTGAAGATCCTCATCCAAATCCAGCCATTGACACCCTTTTTATTGATCATGTTTGTGACCCACAGCCTTCCCGGATCACATCATATCAATCTCCAACTTGTTCTGTGAAGTCAAGTCTATCTACCATTGCACAATATGCATCGTGTATTGGAGGATCTTTCCCTGCAAAGAACCCAGCTACTGAGGTTATTGAGGATTCTGATGTTTTATCCAAAAGGATGCCCATTGTAACTGGGGACGAGGTAACATCAACTATCACTGATCATGGCCAAAATCAGATTTCGGAACTTGCCATATCCAAAAATCTTCCCAGTACTGTTGTCAGTACATTGAATGAGCGGTCTTCTCCAGTGAGCATGGATTTTCATCCCGTTTGGCTTACTGTTCTTCTAGTTGGAACTTCTGCTGGGGAAATAGGACTGTGGGATATTAGCTGCGGTGGAAAAATGGTTGCCAGAAACTTTAATGTTTGGGATCTTGGAGCATGCTCGATGGCCTTAAAGGTAGCACTAGAAAAAGATTCACATGTCTCTGTTAATCGTGTGTTATGGAGTCCGGATGGTTTCTTATTTGGGGTTGCATATTCAAAACATGTTGTGCAATTATATTCTTATATTGGAGGAGATGAACTGGATCAAATATTGGAAATTGATGCTCATTTGGGTGGTGTAAATGATTTAGCATTCTCTTCTCCTGAAGGGGAACTTTTGGTCATAACATGTGGTGATGACAGGACCATCAAGGTGTGGGATGTGGTTAGTGGTAACCGATGCTTTACTTTTGAAGGTCATGATGCTCCTGTTTATTCCATCTGTCCTCATATCAAAGACAATATCCCTTTTATCTTTTCAGTCTCAACAGTTGGCAGGATCAAAGTGTGGTTATATGACAATTTAGGAGACAGAATTGATTATGATGCTCCTGGTCTTGGTTGCACCAAAATGGCTTACAGTGCTGATGAGAGGCTTTTTTCATGTGGGACCAGTAAAGGAGGGGAATCATATCTTGTTGAATGGAATGATACTGTTGGTTGTTTCAAAAGAGTTTACAAAGGACTTGGGCCAAACCCATTGTCTGCCGTGCAATTCGATATAACCAGTAACCAGCTTTTGGCTGCTGGTGATGACCATATGGTTAAATTTTGGCATATGGACAACACTCAACTTTTGAATACCATTGATGCTGATGGTGGATTGCCAGCAAATCCACATATTCGCTTCAACAGTGATGGCACCCTATTAGCTGTTTCTTCAAGTGGAAACCAAATCAAGATACTGGCAAATGCTAATGGTCTTGATTTGCTGCAAGGATGTGGGAATGATTCTATCGATGCCTCTGGAGTTGAATCTGAAACTTCAAGAAAGAATGGGAATGTGGCAGAAGTGAATTCAAAAACAACAGGAGAAGCCACTTACGGAGAAAAGGCATGGGAAACTATTGAGAGACCTTCTCAATGCCAATCTTTGCAGCTATGTGCTGATGTCAAAAGAACCAAGATTTCCAAGCTGATCTACACCAATGCTGGTGACGCCATTTTGGCATTAACATCAAATGCCATTCATCTACTCTGGAAATGGCCGCAGAACGATCTTAGTTCTAGTGGAAAGGCAACTACCAAGGTTCAACCTCTTTTATGGCGACCAACGAGCGGCTTACAGCTGATGAGCAATGACCTTTCCAGCACCAACCCTGAAGAGGCCATGCCATGCTTTGCTTTGTCCAAGAATGATTCATATCTTATGTCAGCATCAGGAGGGATGATCTCCCTTTTTAATGTTATATCGTTTAAGACAATGAGGAAAGTCATGGCTCCACCACCTGCAGCAACATGCCTTGCTTTTGATCCTCGAGACAACAATGTAGTTGCTATTGGCATGGATGATTCCACAATTATGATATATAATGCTCGCTTGGATAAGGTCACAAAAACGCTTGCTGGTCACACCAAAAGAGTCACTTCCCTTGCCTTCTCTACCTCTATGAAATCACTTGTTTCTTCTGGTGCTGATGCTCAGATTTTTGTGTGGAATGCTGATGAGTGGAAAATACAGAGAAGCCAAGTGCTGCAGATTCCAGATTGGAACAAATTGAGAGCATTATCAGACTCAGATACCTATGTCCAGTTTCACGAGGATCAGCTGCATTTTCTAATCGTACACAAGACTCACCTTGCAATATACGAAGTGAAAGAACTAGGATGTGTAAACGAGTGGGTCTCAGATTCTTCTGTACCAATTTCCCATGCAACGTTCTCATGTGATAGCCAGACAGTATATGCCAGCTTTGTGGATGGAACCATAGGCATATTTGACGCTTCTGTTCTTGTACTATTCTGTCTAATCAAGCCCTCCGCTTACCTTCCTACCGATATAAGTTGCAGTTATACTGTGTACCCAGTCGTCATTGCTGGGCATCCACAAAAACCAACCGAGTTTGCCGTTGGACTCTCTAATGGTGAAGTTCATGTGCTTGAGCCTTTAGAATCAGAAGGCAAATGGGGTATATTGCCCACGGTTAAAAGGAATTTGAAGAGCAGTGCGCCTGCAAAACCTACCTTTGGGGGCTTCTTGTATGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010051 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048523 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0090421 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1089

Amino Acids

120.02

Weight (kDa)

5.4

Isoelectric Point (pI)

35.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR1-like_2nd	PF21889	83 - 206	1.8e-53	TPR1-like, CTLH-containing domain
zf_topless	PF21359	209 - 249	2.5e-21	TOPLESS, zinc finger domain
Beta-prop_WDR3_2nd	PF25172	306 - 546	4.3e-06	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	384 - 525	5.3e-14	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	385 - 501	8.7e-12	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	407 - 524	4.9e-13	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	421 - 527	1.9e-08	WDHD1 first WD40 domain
WD40	PF00400	444 - 481	7.7e-07	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	544 - 661	1.3e-07	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	787 - 1003	3.4e-17	WDHD1 first WD40 domain
WDR55	PF24796	788 - 908	8.5e-07	WDR55
Beta-prop_EML_2	PF23414	791 - 926	2e-10	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	792 - 912	2.3e-11	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	796 - 1002	3.6e-20	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	800 - 944	1.6e-13	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	804 - 912	1.1e-12	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	811 - 908	8e-15	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	811 - 913	3.5e-10	WDR36/Utp21 first beta-propeller
Beta-prop_IFT140_1st	PF23383	812 - 918	3.3e-08	IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	814 - 908	3e-09	WDR36/Utp21 second beta-propeller domain
WD40_Prp19	PF24814	836 - 1002	9.6e-16	Prp19 WD40 domain
Beta-prop_WDR19_1st	PF23389	840 - 961	1.5e-06	WDR19 first beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	842 - 907	6.6e-07	MABP1/WDR62 second WD40 domain
WD40	PF00400	871 - 907	3.3e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0017445)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr6g0297391
rosa_laevigata	RLG00000011603
rosa_multiflora	Rmu_sc0004989.1_g000003
rosa_roxburghii	Rroxscaffold_7G00170560
rosa_rugosa	Rorug06G0271300 Rorug06G0271400 Rorug06G0271500 Rorug06G0271600
rosa_samantha	Rh6AG382800 Rh6BG391100 Rh6CG396000 Rh6DG383300
rosa_wichuraiana	Rw6G033340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	198
AarI	CACCTGC	1 cut(s)	2518
Acc36I	ACCTGC	1 cut(s)	2518
AccB1I	GGYRCC	1 cut(s)	1922
AccB7I	CCANNNNNTGG	1 cut(s)	1801
AccBSI	CCGCTC	2 cut(s)	1030, 2340
AccI	GTMKAC	1 cut(s)	613
AccIII	TCCGGA	1 cut(s)	1246
AciI	CCGC	6 cut(s)	207, 1030, 1128, 2270, 2340, 3042
AclI	AACGTT	1 cut(s)	2939
AclWI	GGATC	8 cut(s)	692, 773, 853, 1173, 1332, 1568, 2528, 2832
AcoI	YGGCCR	2 cut(s)	958, 2267
AcsI	RAATTY	4 cut(s)	620, 1829, 2077, 3214
AcuI	CTGAAG	3 cut(s)	109, 1407, 2403
AcyI	GRCGYC	2 cut(s)	652, 2220
AfaI	GTAC	7 cut(s)	338, 1006, 1018, 2847, 2922, 3018, 3083
AfiI	CCNNNNNNNGG	5 cut(s)	1619, 1801, 2339, 2386, 3248
AflIII	ACRYGT	3 cut(s)	1216, 1282, 1409
AjnI	CCWGG	1 cut(s)	1612
Alw26I	GTCTC	7 cut(s)	90, 1225, 1549, 1584, 2128, 2535, 2911
AlwI	GGATC	8 cut(s)	692, 773, 853, 1173, 1332, 1568, 2528, 2832
AlwNI	CAGNNNCTG	1 cut(s)	872
Ama87I	CYCGRG	1 cut(s)	2538
Aor13HI	TCCGGA	1 cut(s)	1246
AoxI	GGCC	6 cut(s)	63, 958, 1185, 1751, 2267, 2388
ApeKI	GCWGC	8 cut(s)	679, 1125, 1805, 1993, 2158, 2513, 2755, 2830
ApoI	RAATTY	4 cut(s)	620, 1829, 2077, 3214
ArsI	GACNNNNNNTTYG	2 cut(s)	1748, 1780
AspLEI	GCGC	1 cut(s)	3232
AspS9I	GGNCC	3 cut(s)	1425, 1671, 1751
AsuC2I	CCSGG	1 cut(s)	763
AsuHPI	GGTGA	6 cut(s)	23, 1427, 1823, 2228, 2849, 3158
AvaI	CYCGRG	1 cut(s)	2538
AvaII	GGWCC	2 cut(s)	1425, 1671
BaeI	ACNNNNGTAYC	2 cut(s)	2793, 2826
BalI	TGGCCA	1 cut(s)	960
BanI	GGYRCC	1 cut(s)	1922
BarI	GAAGNNNNNNTAC	2 cut(s)	359, 391
BauI	CACGAG	1 cut(s)	2819
BbsI	GAAGAC	1 cut(s)	1026
BbvI	GCAGC	8 cut(s)	691, 1112, 1792, 1980, 2170, 2525, 2742, 2817
BccI	CCATC	7 cut(s)	1177, 1244, 1436, 1508, 1871, 1913, 2975
BceAI	ACGGC	3 cut(s)	665, 1754, 3113
BciT130I	CCWGG	1 cut(s)	1614
BclI	TGATCA	2 cut(s)	736, 952
BcnI	CCSGG	1 cut(s)	763
BcoDI	GTCTC	7 cut(s)	90, 1225, 1549, 1584, 2128, 2535, 2911
BfaI	CTAG	4 cut(s)	1082, 1202, 2289, 2885
BfmI	CTRYAG	2 cut(s)	2511, 2756
BfuAI	ACCTGC	1 cut(s)	2518
BglI	GCCNNNNNGGC	1 cut(s)	2228
BglII	AGATCT	1 cut(s)	348
BmcAI	AGTACT	2 cut(s)	338, 1006
Bme1390I	CCNGG	2 cut(s)	763, 1614
Bme18I	GGWCC	2 cut(s)	1425, 1671
BmeT110I	CYCGRG	1 cut(s)	2538
BmgT120I	GGNCC	3 cut(s)	1425, 1671, 1751
BmiI	GGNNCC	5 cut(s)	154, 1672, 1924, 2502, 2986
BmrFI	CCNGG	2 cut(s)	763, 1614
BmrI	ACTGGG	3 cut(s)	933, 996, 3080
BmuI	ACTGGG	3 cut(s)	933, 996, 3080
BpiI	GAAGAC	1 cut(s)	1026
BplI	GAGNNNNNCTC	6 cut(s)	509, 541, 2125, 2157, 2891, 2923
BpmI	CTGGAG	2 cut(s)	1023, 2049
BpuEI	CTTGAG	3 cut(s)	166, 239, 3182
BpuMI	CCSGG	1 cut(s)	763
Bsa29I	ATCGAT	1 cut(s)	2019
BsaBI	GATNNNNATC	3 cut(s)	702, 1056, 2424
BsaHI	GRCGYC	2 cut(s)	652, 2220
BsaI	GGTCTC	2 cut(s)	2128, 2911
BsaJI	CCNNGG	5 cut(s)	168, 408, 607, 2307, 3201
BsaWI	WCCGGW	1 cut(s)	1246
Bsc4I	CCNNNNNNNGG	5 cut(s)	1619, 1801, 2339, 2386, 3248
Bse118I	RCCGGY	1 cut(s)	308
Bse3DI	GCAATG	4 cut(s)	695, 816, 2365, 3093
Bse8I	GATNNNNATC	3 cut(s)	702, 1056, 2424
BseAI	TCCGGA	1 cut(s)	1246
BseBI	CCWGG	1 cut(s)	1614
BseCI	ATCGAT	1 cut(s)	2019
BseDI	CCNNGG	5 cut(s)	168, 408, 607, 2307, 3201
BseJI	GATNNNNATC	3 cut(s)	702, 1056, 2424
BseLI	CCNNNNNNNGG	5 cut(s)	1619, 1801, 2339, 2386, 3248
BseMI	GCAATG	4 cut(s)	695, 816, 2365, 3093
BseMII	CTCAG	4 cut(s)	864, 2717, 2811, 2922
BseRI	GAGGAG	1 cut(s)	1326
BseXI	GCAGC	8 cut(s)	691, 1112, 1792, 1980, 2170, 2525, 2742, 2817
BseYI	CCCAGC	3 cut(s)	865, 1097, 3098
BshFI	GGCC	6 cut(s)	65, 960, 1187, 1753, 2269, 2390
BshNI	GGYRCC	1 cut(s)	1922
BshVI	ATCGAT	1 cut(s)	2019
BsiHKCI	CYCGRG	1 cut(s)	2538
BsiSI	CCGG	3 cut(s)	309, 763, 1247
BslFI	GGGAC	2 cut(s)	941, 1684
BslI	CCNNNNNNNGG	5 cut(s)	1619, 1801, 2339, 2386, 3248
BsmAI	GTCTC	7 cut(s)	90, 1225, 1549, 1584, 2128, 2535, 2911
BsmFI	GGGAC	2 cut(s)	941, 1684
BsmI	GAATGC	2 cut(s)	1373, 2725
BsnI	GGCC	6 cut(s)	65, 960, 1187, 1753, 2269, 2390
Bso31I	GGTCTC	2 cut(s)	2128, 2911
BsoBI	CYCGRG	1 cut(s)	2538
Bsp13I	TCCGGA	1 cut(s)	1246
BspACI	CCGC	6 cut(s)	207, 1030, 1128, 2270, 2340, 3042
BspANI	GGCC	6 cut(s)	65, 960, 1187, 1753, 2269, 2390
BspCNI	CTCAG	4 cut(s)	865, 2716, 2810, 2921
BspDI	ATCGAT	1 cut(s)	2019
BspEI	TCCGGA	1 cut(s)	1246
BspHI	TCATGA	1 cut(s)	1480
BspLI	GGNNCC	5 cut(s)	154, 1672, 1924, 2502, 2986
BspMAI	CTGCAG	2 cut(s)	2515, 2760
BspMI	ACCTGC	1 cut(s)	2518
BspPI	GGATC	8 cut(s)	692, 773, 853, 1173, 1332, 1568, 2528, 2832
BspQI	GCTCTTC	4 cut(s)	128, 255, 456, 3215
BspT107I	GGYRCC	1 cut(s)	1922
BspTNI	GGTCTC	2 cut(s)	2128, 2911
BsrBI	CCGCTC	2 cut(s)	1030, 2340
BsrDI	GCAATG	4 cut(s)	695, 816, 2365, 3093
BsrFI	RCCGGY	1 cut(s)	308
BssAI	RCCGGY	1 cut(s)	308
BssECI	CCNNGG	5 cut(s)	168, 408, 607, 2307, 3201
BssNI	GRCGYC	2 cut(s)	652, 2220
BssSI	CACGAG	1 cut(s)	2819
BssT1I	CCWWGG	2 cut(s)	168, 2307
Bst2BI	CACGAG	1 cut(s)	2819
Bst2UI	CCWGG	1 cut(s)	1614
Bst6I	CTCTTC	6 cut(s)	128, 255, 456, 1384, 2379, 3215
BstACI	GRCGYC	2 cut(s)	652, 2220
BstAPI	GCANNNNNTGC	2 cut(s)	685, 827
BstC8I	GCNNGC	7 cut(s)	310, 1177, 1890, 2599, 2625, 2971, 3234
BstDEI	CTNAG	7 cut(s)	272, 345, 873, 2282, 2703, 2797, 2908
BstDSI	CCRYGG	2 cut(s)	607, 3201
BstEII	GGTNACC	1 cut(s)	1454
BstHHI	GCGC	1 cut(s)	3232
BstMAI	GTCTC	7 cut(s)	90, 1225, 1549, 1584, 2128, 2535, 2911
BstMWI	GCNNNNNNNGC	5 cut(s)	685, 827, 1643, 2219, 2228
BstNI	CCWGG	1 cut(s)	1614
BstNSI	RCATGY	6 cut(s)	217, 1179, 1220, 1286, 1413, 2523
BstPI	GGTNACC	1 cut(s)	1454
BstSCI	CCNGG	2 cut(s)	761, 1612
BstSFI	CTRYAG	2 cut(s)	2511, 2756
BstV1I	GCAGC	8 cut(s)	691, 1112, 1792, 1980, 2170, 2525, 2742, 2817
BstV2I	GAAGAC	1 cut(s)	1026
BstX2I	RGATCY	4 cut(s)	348, 697, 845, 1165
BstXI	CCANNNNNNTGG	1 cut(s)	2977
BstYI	RGATCY	4 cut(s)	348, 697, 845, 1165
Bsu15I	ATCGAT	1 cut(s)	2019
BsuRI	GGCC	6 cut(s)	65, 960, 1187, 1753, 2269, 2390
BsuTUI	ATCGAT	1 cut(s)	2019
BtgI	CCRYGG	2 cut(s)	607, 3201
BtsI	GCAGTG	1 cut(s)	3232
BtsIMutI	CAGTG	5 cut(s)	948, 1047, 1648, 1921, 3232
BveI	ACCTGC	1 cut(s)	2518
Cac8I	GCNNGC	7 cut(s)	310, 1177, 1890, 2599, 2625, 2971, 3234
CaiI	CAGNNNCTG	1 cut(s)	872
CciI	TCATGA	1 cut(s)	1480
CfoI	GCGC	1 cut(s)	3232
Cfr10I	RCCGGY	1 cut(s)	308
Cfr13I	GGNCC	3 cut(s)	1425, 1671, 1751
ClaI	ATCGAT	1 cut(s)	2019
CseI	GACGC	3 cut(s)	641, 2228, 3011
Csp6I	GTAC	7 cut(s)	337, 1005, 1017, 2846, 2921, 3017, 3082
CviQI	GTAC	7 cut(s)	337, 1005, 1017, 2846, 2921, 3017, 3082
DdeI	CTNAG	7 cut(s)	272, 345, 873, 2282, 2703, 2797, 2908
EaeI	YGGCCR	2 cut(s)	958, 2267
Eam1104I	CTCTTC	6 cut(s)	128, 255, 456, 1384, 2379, 3215
EarI	CTCTTC	6 cut(s)	128, 255, 456, 1384, 2379, 3215
Eco130I	CCWWGG	2 cut(s)	168, 2307
Eco31I	GGTCTC	2 cut(s)	2128, 2911
Eco47I	GGWCC	2 cut(s)	1425, 1671
Eco57I	CTGAAG	3 cut(s)	109, 1407, 2403
Eco88I	CYCGRG	1 cut(s)	2538
Eco91I	GGTNACC	1 cut(s)	1454
EcoO65I	GGTNACC	1 cut(s)	1454
EcoRI	GAATTC	1 cut(s)	2077
EcoRII	CCWGG	1 cut(s)	1612
EcoT14I	CCWWGG	2 cut(s)	168, 2307
EcoT22I	ATGCAT	1 cut(s)	832
ErhI	CCWWGG	2 cut(s)	168, 2307
FalI	AAGNNNNNCTT	6 cut(s)	338, 370, 1380, 1412, 2632, 2664
FaqI	GGGAC	2 cut(s)	941, 1684
FauNDI	CATATG	3 cut(s)	684, 1821, 1839
FbaI	TGATCA	2 cut(s)	736, 952
FblI	GTMKAC	1 cut(s)	613
FspBI	CTAG	4 cut(s)	1082, 1202, 2289, 2885
GlaI	GCGC	1 cut(s)	3231
GsaI	CCCAGC	3 cut(s)	869, 1101, 3102
GsuI	CTGGAG	2 cut(s)	1023, 2049
HaeIII	GGCC	6 cut(s)	65, 960, 1187, 1753, 2269, 2390
HapII	CCGG	3 cut(s)	309, 763, 1247
HgaI	GACGC	3 cut(s)	641, 2228, 3011
HhaI	GCGC	1 cut(s)	3232
Hin1I	GRCGYC	2 cut(s)	652, 2220
Hin6I	GCGC	1 cut(s)	3230
HinP1I	GCGC	1 cut(s)	3230
HindIII	AAGCTT	1 cut(s)	667
HpaII	CCGG	3 cut(s)	309, 763, 1247
HphI	GGTGA	6 cut(s)	23, 1427, 1823, 2228, 2849, 3158
Hpy166II	GTNNAC	5 cut(s)	614, 1738, 2848, 2896, 3082
Hpy8I	GTNNAC	5 cut(s)	614, 1738, 2848, 2896, 3082
HpyCH4IV	ACGT	1 cut(s)	2939
HpyF10VI	GCNNNNNNNGC	5 cut(s)	685, 827, 1643, 2219, 2228
HpyF3I	CTNAG	7 cut(s)	272, 345, 873, 2282, 2703, 2797, 2908
HpySE526I	ACGT	1 cut(s)	2939
Hsp92I	GRCGYC	2 cut(s)	652, 2220
HspAI	GCGC	1 cut(s)	3230
Kpn2I	TCCGGA	1 cut(s)	1246
KroI	GCCGGC	1 cut(s)	308
KroNI	GCCGGC	1 cut(s)	310
Ksp22I	TGATCA	2 cut(s)	736, 952
LguI	GCTCTTC	4 cut(s)	128, 255, 456, 3215
LmnI	GCTCC	5 cut(s)	83, 1172, 1492, 1615, 2506
Lsp1109I	GCAGC	8 cut(s)	691, 1112, 1792, 1980, 2170, 2525, 2742, 2817
MaeI	CTAG	4 cut(s)	1082, 1202, 2289, 2885
MaeII	ACGT	1 cut(s)	2939
MbiI	CCGCTC	2 cut(s)	1030, 2340
MfeI	CAATTG	1 cut(s)	108
MflI	RGATCY	4 cut(s)	348, 697, 845, 1165
MlsI	TGGCCA	1 cut(s)	960
MluNI	TGGCCA	1 cut(s)	960
MlyI	GAGTC	8 cut(s)	153, 475, 527, 1252, 2652, 2789, 2848, 3129
MmeI	TCCRAC	4 cut(s)	31, 809, 1066, 3112
Mox20I	TGGCCA	1 cut(s)	960
Mph1103I	ATGCAT	1 cut(s)	832
MroI	TCCGGA	1 cut(s)	1246
MroNI	GCCGGC	1 cut(s)	308
MscI	TGGCCA	1 cut(s)	960
MseI	TTAA	9 cut(s)	660, 1155, 1190, 1227, 1827, 2234, 2463, 2478, 3207
MslI	CAYNNNNRTG	5 cut(s)	744, 1353, 1433, 1631, 2582
Msp20I	TGGCCA	1 cut(s)	960
MspA1I	CMGCKG	2 cut(s)	2350, 2830
MspI	CCGG	3 cut(s)	309, 763, 1247
MspR9I	CCNGG	2 cut(s)	763, 1614
MunI	CAATTG	1 cut(s)	108
Mva1269I	GAATGC	2 cut(s)	1373, 2725
MvaI	CCWGG	1 cut(s)	1614
MwoI	GCNNNNNNNGC	5 cut(s)	685, 827, 1643, 2219, 2228
NaeI	GCCGGC	1 cut(s)	310
NciI	CCSGG	1 cut(s)	763
NdeI	CATATG	3 cut(s)	684, 1821, 1839
NgoMIV	GCCGGC	1 cut(s)	308
NlaIV	GGNNCC	5 cut(s)	154, 1672, 1924, 2502, 2986
NmeAIII	GCCGAG	1 cut(s)	433
NmuCI	GTSAC	6 cut(s)	519, 746, 2216, 2611, 2630, 2644
NsiI	ATGCAT	1 cut(s)	832
NspI	RCATGY	6 cut(s)	217, 1179, 1220, 1286, 1413, 2523
PaeI	GCATGC	1 cut(s)	1179
PaeR7I	CTCGAG	1 cut(s)	2538
PagI	TCATGA	1 cut(s)	1480
PaqCI	CACCTGC	1 cut(s)	2518
PciI	ACATGT	3 cut(s)	1216, 1282, 1409
PciSI	GCTCTTC	4 cut(s)	128, 255, 456, 3215
PcsI	WCGNNNNNNNCGW	1 cut(s)	636
PctI	GAATGC	2 cut(s)	1373, 2725
PdiI	GCCGGC	1 cut(s)	310
PflMI	CCANNNNNTGG	1 cut(s)	1801
PfoI	TCCNGGA	1 cut(s)	761
PleI	GAGTC	8 cut(s)	153, 475, 526, 1251, 2651, 2789, 2848, 3129
PpsI	GAGTC	8 cut(s)	153, 475, 526, 1251, 2651, 2789, 2848, 3129
PscI	ACATGT	3 cut(s)	1216, 1282, 1409
PsiI	TTATAA	1 cut(s)	198
Psp1406I	AACGTT	1 cut(s)	2939
Psp6I	CCWGG	1 cut(s)	1612
PspEI	GGTNACC	1 cut(s)	1454
PspFI	CCCAGC	3 cut(s)	865, 1097, 3098
PspGI	CCWGG	1 cut(s)	1612
PspN4I	GGNNCC	5 cut(s)	154, 1672, 1924, 2502, 2986
PspPI	GGNCC	3 cut(s)	1425, 1671, 1751
PstI	CTGCAG	2 cut(s)	2515, 2760
PstNI	CAGNNNCTG	1 cut(s)	872
PsuI	RGATCY	4 cut(s)	348, 697, 845, 1165
PvuII	CAGCTG	2 cut(s)	2350, 2830
RsaI	GTAC	7 cut(s)	338, 1006, 1018, 2847, 2922, 3018, 3083
RsaNI	GTAC	7 cut(s)	337, 1005, 1017, 2846, 2921, 3017, 3082
RseI	CAYNNNNRTG	5 cut(s)	744, 1353, 1433, 1631, 2582
SapI	GCTCTTC	4 cut(s)	128, 255, 456, 3215
SaqAI	TTAA	9 cut(s)	660, 1155, 1190, 1227, 1827, 2234, 2463, 2478, 3207
Sau96I	GGNCC	3 cut(s)	1425, 1671, 1751
ScaI	AGTACT	2 cut(s)	338, 1006
SchI	GAGTC	8 cut(s)	153, 475, 527, 1252, 2652, 2789, 2848, 3129
ScrFI	CCNGG	2 cut(s)	763, 1614
SfcI	CTRYAG	2 cut(s)	2511, 2756
Sfr274I	CTCGAG	1 cut(s)	2538
SinI	GGWCC	2 cut(s)	1425, 1671
SlaI	CTCGAG	1 cut(s)	2538
SmiMI	CAYNNNNRTG	5 cut(s)	744, 1353, 1433, 1631, 2582
SmlI	CTYRAG	4 cut(s)	145, 218, 2538, 3161
SmoI	CTYRAG	4 cut(s)	145, 218, 2538, 3161
SphI	GCATGC	1 cut(s)	1179
SsiI	CCGC	6 cut(s)	207, 1030, 1128, 2270, 2340, 3042
SspI	AATATT	1 cut(s)	1332
SspMI	CTAG	4 cut(s)	1082, 1202, 2289, 2885
StyD4I	CCNGG	2 cut(s)	761, 1612
StyI	CCWWGG	2 cut(s)	168, 2307
TaiI	ACGT	1 cut(s)	2942
TaqI	TCGA	6 cut(s)	324, 341, 1181, 1779, 2019, 2539
TatI	WGTACW	4 cut(s)	336, 1004, 1016, 3016
TauI	GCSGC	3 cut(s)	209, 2272, 2343
Tru1I	TTAA	9 cut(s)	660, 1155, 1190, 1227, 1827, 2234, 2463, 2478, 3207
Tru9I	TTAA	9 cut(s)	660, 1155, 1190, 1227, 1827, 2234, 2463, 2478, 3207
TscAI	CASTG	5 cut(s)	955, 1047, 1648, 1921, 3232
TseFI	GTSAC	6 cut(s)	519, 746, 2216, 2611, 2630, 2644
TseI	GCWGC	8 cut(s)	679, 1125, 1805, 1993, 2158, 2513, 2755, 2830
Tsp45I	GTSAC	6 cut(s)	519, 746, 2216, 2611, 2630, 2644
TspGWI	ACGGA	2 cut(s)	596, 2121
TspRI	CASTG	5 cut(s)	955, 1047, 1648, 1921, 3232
Van91I	CCANNNNNTGG	1 cut(s)	1801
VpaK11BI	GGWCC	2 cut(s)	1425, 1671
XapI	RAATTY	4 cut(s)	620, 1829, 2077, 3214
XceI	RCATGY	6 cut(s)	217, 1179, 1220, 1286, 1413, 2523
XhoI	CTCGAG	1 cut(s)	2538
XmiI	GTMKAC	1 cut(s)	613
XspI	CTAG	4 cut(s)	1082, 1202, 2289, 2885
ZrmI	AGTACT	2 cut(s)	338, 1006
Zsp2I	ATGCAT	1 cut(s)	832

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.