Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0017445

Physical Location & Seq

Genomic Coordinates Reverse (-)

57814974 .. 57821931

Gene Structure 6958 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G033340.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3135 bp

ATGTCTAATCTCAGTGAAGATCTTGTGTTCTTGGTCTTAAAGTATTTAGATGAAGAAGGTTATAAGGAAGCCGCCCACATGCTTGAGCGTGAAAGTGGGCTTTACTTTGACATGAATTATTTTGAAGAGCTGGTGCTTAGTGGAAAATGGGATGAAGCAGAGAAATACCTCGCCGGCTTCACAAAGCTCGATGACAATAAGTACTCGACTAAGATCTACTTTGAAATCAGGAAGCAACATTACTTTGAGGCACTTGACAAAAAATGTCGTGCCGAGGCTTTAGATGTCCTCACAAATGATCTACAAGTTTTTGCTAAAGGTAATGAAGAGCTATTCAAAGAAATGACTCAACTTATGACATTTGAGAATATAAGGGAACATGAGTCACTCTCTATGTATAGAGATACAAAGTCTGCAAGGAAGGTTATGATGAAAGAAATCAGGAGGGTAATTGAGGCAAATCCAATTTTCCGTGGTAGACTGGAATTTCCTATCGTAAAAGGCGAGAGATTACGGCGTCTGATTAACCAAAGCTTGAATTGGCAGCATATGCAATGTGAAGATCCTCATCCAAATCCAGCCATTGACACCCTTTTTATTGATCATGTTTGTGACCCACAGCCTTCCCGGACCACATCATATCAATCTCCAACTTGTTCTGTGAAGTCAAGTCTATCTACCATTGCACAATATGCATCGTGTATTGGAGGATCTTTCCCTGCAAAGAACCCAGCTACTGAGGTTATTGAGGATTCTGATGTTTTATCCAAAAGGATGCCCATTGTAACTGGGGATGAGGTAACATCAACTATCACTGATCATGGCCAAAATCAGATTTCGGAACTTGCCATATCCAAAAATCTTCCCAGTACTGTTGTCAGTACATTGAATGAGCGGTCTTCTCCAGTGAGCATGGATTTTCATCCCGTTTGGCTTACTGTTCTTCTAGTTGGAACTTCTGCTGGGGAAATAGGACTGTGGGATATTAGCTGCGGTGGAAAAATGGTTGCCAGAAACTTTAATGTTTGGGATCTTGGAGCATGCTCGATGGCCTTAAAGGTAGCACTAGAAAAAGATTCACATGTCTCTGTTAATCGTGTGTTATGGAGTCCGGATGGTTTCTTATTTGGGGTTGCATATTCAAAACATGTTGTGCAATTATATTCTTATATTGGAGGAGATGAACTGGATCAAATATTGGAAATTGATGCTCATTTGGGTGGTGTAAATGATTTAGCATTCTCTTCTCCTGAAGGGGAACTTTTGGTCATAACATGTGGTGATGACAGGACCATCAAGGTGTGGGATGTGGTTAGTGGTAACCGATGCTTTACTTTTGAAGGTCATGATGCTCCTGTTTATTCCATCTGTCCTCATATCAAAGACAATATCCCTTTTATCTTTTCAGTCTCAACAGTTGGCAGGATCAAAGTGTGGTTATATGACAATTTAGGAGACAGAATTGATTATGATGCTCCTGGTCTTGGTTGCACCAAAATGGCTTACAGTGCTGATGAGAGGCTTTTTTCATGTGGGACCAGTAAAGGAGGGGAATCATATCTTGTTGAATGGAATGATACTGTTGGTTGTTTCAAAAGAGTTTACAAAGGACTTGGGCCAAACCCATTGTCTGCCGTGCAATTCGATATAACCAGTAACCAGCTTTTGGCTGCTGGTGATGACCATATGGTTAAATTTTGGCATATGGACAACACTCAACTTTTGAATACCATTGATGCTGATGGTGGATTGCCAGCAAATCCACATATTCGCTTCAACAGTGATGGCACCCTATTAGCTGTTTCTTCAAGTGGAAACCAAATCAAGATACTGGCAAATGCTAATGGTCTTGATTTGCTGCAAGGATGTGGGAATGATTCTATCGATGCCTCTGGAGTTGAATCTGAAACTTCAAGAAAGAATGGGAATGTGGCAGAAGTGAATTCAAAAACAACAGGAGAAGCCACTTACGGAGAAAAGGCATGGGAAACTATTGAGAGACCTTCTCAATGCCAATCTTTGCAGCTATGTGCTGATGTCAAAAGAACCAAGATTTCCAAGCTGATCTACACCAATGCTGGTGACGCCATTTTGGCATTAACATCAAATGCCATTCATCTACTCTGGAAATGGCCGCAGAACGATCTTAGTTCTAGTGGAAAGGCAACTACCAAGGTTCAACCTCTTTTATGGCGACCAACGAGCGGCTTACAGCTGATGAGCAATGACCTTTCCAGCACCAACCCTGAAGAGGCCATGCCATGCTTTGCTTTGTCCAAGAATGATTCATATCTTATGTCAGCATCAGGAGGGATGATCTCCCTTTTTAATGTTATATCGTTTAAGACAATGAGGAAAGTCATGGCTCCACCACCTGCAGCAACATGCCTTGCTTTTGATCCTCGAGACAACAATGTAGTTGCTATTGGCATGGATGATTCCACAATTATGATATATAATGCTCGCTTGGATAAGGTCACAAAAACGCTTGCTGGTCACACCAAAAGAGTCACTTCCCTTGCCTTCTCTACCTCTATGAAATCACTTGTTTCTTCTGGTGCTGATGCTCAGATTTTTGTGTGGAATGCTGATGAGTGGAAAATACAGAGAAGCCAAGTGCTGCAGATTCCAGATTGGAACAAATTGAGAGCATTATCAGACTCAGATACCTATGTCCAGTTTCACGAGGATCAGCTGCATTTTCTAATCGTACACAAGACTCACCTTGCAATATACGAAGTGAAAGAACTAGGATGTGTAAACGAGTGGGTCTCAGATTCTTCTGTACCAATTTCCCATGCAACGTTCTCATGTGATAGCCAGACAGTATATGCCAGCTTTGTGGATGGAACCATAGGCATATTTGACGCTTCTGTTCTTGTACTATTCTGTCTAATCAAGCCCTCCGCTTACCTTCCTACCGATATAAGTTGCAGTTATACTGTGTACCCAGTCGTCATTGCTGGGCATCCACAAAAACCAACCGAGTTTGCCGTTGGACTCTCTAATGGTGAAGTTCATGTGCTTGAGCCTTTAGAATCAGAAGGCAAATGGGGTATATTGCCCACGGTTAAAAGGAATTTGAAGAGCAGTGCGCCTGCAAAACCTACCTTTGGGGGCTTCTTGTATGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010051 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048523 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0090421 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1044

Amino Acids

115.59

Weight (kDa)

5.26

Isoelectric Point (pI)

38.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	5 - 33	5e-06	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	38 - 161	1.7e-53	TPR1-like, CTLH-containing domain
zf_topless	PF21359	164 - 204	2.4e-21	TOPLESS, zinc finger domain
Beta-prop_WDR3_2nd	PF25172	261 - 501	4e-06	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	339 - 481	5e-14	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	340 - 456	8.7e-12	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	362 - 480	4.2e-13	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	376 - 482	1.8e-08	WDHD1 first WD40 domain
WD40	PF00400	399 - 436	7.4e-07	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	499 - 616	1.3e-07	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	742 - 958	3.2e-17	WDHD1 first WD40 domain
WDR55	PF24796	743 - 863	8e-07	WDR55
Beta-prop_EML_2	PF23414	746 - 881	1.9e-10	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	747 - 867	2.3e-11	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	751 - 957	3.4e-20	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	755 - 899	1.5e-13	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	759 - 867	1e-12	THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	766 - 868	3.6e-10	WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st	PF25173	766 - 863	7.6e-15	WDR3 first beta-propeller domain
Beta-prop_IFT140_1st	PF23383	767 - 873	3.1e-08	IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	769 - 863	2.9e-09	WDR36/Utp21 second beta-propeller domain
WD40_Prp19	PF24814	791 - 957	9e-16	Prp19 WD40 domain
Beta-prop_WDR19_1st	PF23389	794 - 916	1.4e-06	WDR19 first beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	797 - 862	6.2e-07	MABP1/WDR62 second WD40 domain
WD40	PF00400	826 - 862	3.2e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017445)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr6g0297391
rosa_laevigata	RLG00000011603
rosa_multiflora	Rmu_sc0004989.1_g000003
rosa_roxburghii	Rroxscaffold_7G00170560
rosa_rugosa	Rorug06G0271300 Rorug06G0271400 Rorug06G0271500 Rorug06G0271600
rosa_samantha	Rh6AG382800 Rh6BG391100 Rh6CG396000 Rh6DG383300
rosa_wichuraiana	Rw6G033340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	63
AarI	CACCTGC	1 cut(s)	2383
Acc36I	ACCTGC	1 cut(s)	2383
AccB1I	GGYRCC	1 cut(s)	1787
AccB7I	CCANNNNNTGG	1 cut(s)	1666
AccBSI	CCGCTC	2 cut(s)	895, 2205
AccI	GTMKAC	1 cut(s)	478
AccIII	TCCGGA	1 cut(s)	1111
AciI	CCGC	6 cut(s)	72, 895, 993, 2135, 2205, 2907
AclI	AACGTT	1 cut(s)	2804
AclWI	GGATC	7 cut(s)	557, 718, 1038, 1197, 1433, 2393, 2697
AcoI	YGGCCR	2 cut(s)	823, 2132
AcsI	RAATTY	4 cut(s)	485, 1694, 1942, 3079
AcuI	CTGAAG	2 cut(s)	1272, 2268
AcyI	GRCGYC	2 cut(s)	517, 2085
AfaI	GTAC	7 cut(s)	203, 871, 883, 2712, 2787, 2883, 2948
AfiI	CCNNNNNNNGG	5 cut(s)	1484, 1666, 2204, 2251, 3113
AflIII	ACRYGT	3 cut(s)	1081, 1147, 1274
AjnI	CCWGG	1 cut(s)	1477
Alw26I	GTCTC	6 cut(s)	1090, 1414, 1449, 1993, 2400, 2776
AlwI	GGATC	7 cut(s)	557, 718, 1038, 1197, 1433, 2393, 2697
AlwNI	CAGNNNCTG	1 cut(s)	737
Ama87I	CYCGRG	1 cut(s)	2403
Aor13HI	TCCGGA	1 cut(s)	1111
AoxI	GGCC	5 cut(s)	823, 1050, 1616, 2132, 2253
ApeKI	GCWGC	8 cut(s)	544, 990, 1670, 1858, 2023, 2378, 2620, 2695
ApoI	RAATTY	4 cut(s)	485, 1694, 1942, 3079
ArsI	GACNNNNNNTTYG	2 cut(s)	1613, 1645
AspLEI	GCGC	1 cut(s)	3097
AspS9I	GGNCC	4 cut(s)	630, 1290, 1536, 1616
AsuC2I	CCSGG	1 cut(s)	628
AsuHPI	GGTGA	5 cut(s)	1292, 1688, 2093, 2714, 3023
AvaI	CYCGRG	1 cut(s)	2403
AvaII	GGWCC	3 cut(s)	630, 1290, 1536
BaeI	ACNNNNGTAYC	2 cut(s)	2658, 2691
BalI	TGGCCA	1 cut(s)	825
BanI	GGYRCC	1 cut(s)	1787
BarI	GAAGNNNNNNTAC	2 cut(s)	224, 256
BauI	CACGAG	1 cut(s)	2684
BbsI	GAAGAC	1 cut(s)	891
BbvI	GCAGC	8 cut(s)	556, 977, 1657, 1845, 2035, 2390, 2607, 2682
BccI	CCATC	7 cut(s)	1042, 1109, 1301, 1373, 1736, 1778, 2840
BceAI	ACGGC	3 cut(s)	530, 1619, 2978
BciT130I	CCWGG	1 cut(s)	1479
BclI	TGATCA	2 cut(s)	601, 817
BcnI	CCSGG	1 cut(s)	628
BcoDI	GTCTC	6 cut(s)	1090, 1414, 1449, 1993, 2400, 2776
BfaI	CTAG	4 cut(s)	947, 1067, 2154, 2750
BfmI	CTRYAG	2 cut(s)	2376, 2621
BfuAI	ACCTGC	1 cut(s)	2383
BglI	GCCNNNNNGGC	1 cut(s)	2093
BglII	AGATCT	2 cut(s)	19, 213
BmcAI	AGTACT	2 cut(s)	203, 871
Bme1390I	CCNGG	2 cut(s)	628, 1479
Bme18I	GGWCC	3 cut(s)	630, 1290, 1536
BmeT110I	CYCGRG	1 cut(s)	2403
BmgT120I	GGNCC	4 cut(s)	630, 1290, 1536, 1616
BmiI	GGNNCC	4 cut(s)	1537, 1789, 2367, 2851
BmrFI	CCNGG	2 cut(s)	628, 1479
BmrI	ACTGGG	3 cut(s)	798, 861, 2945
BmuI	ACTGGG	3 cut(s)	798, 861, 2945
BpiI	GAAGAC	1 cut(s)	891
BplI	GAGNNNNNCTC	6 cut(s)	374, 406, 1990, 2022, 2756, 2788
BpmI	CTGGAG	2 cut(s)	888, 1914
BpuEI	CTTGAG	2 cut(s)	104, 3047
BpuMI	CCSGG	1 cut(s)	628
Bsa29I	ATCGAT	1 cut(s)	1884
BsaBI	GATNNNNATC	3 cut(s)	567, 921, 2289
BsaHI	GRCGYC	2 cut(s)	517, 2085
BsaI	GGTCTC	2 cut(s)	1993, 2776
BsaJI	CCNNGG	4 cut(s)	273, 472, 2172, 3066
BsaWI	WCCGGW	1 cut(s)	1111
Bsc4I	CCNNNNNNNGG	5 cut(s)	1484, 1666, 2204, 2251, 3113
Bse118I	RCCGGY	1 cut(s)	173
Bse3DI	GCAATG	4 cut(s)	560, 681, 2230, 2958
Bse8I	GATNNNNATC	3 cut(s)	567, 921, 2289
BseAI	TCCGGA	1 cut(s)	1111
BseBI	CCWGG	1 cut(s)	1479
BseCI	ATCGAT	1 cut(s)	1884
BseDI	CCNNGG	4 cut(s)	273, 472, 2172, 3066
BseJI	GATNNNNATC	3 cut(s)	567, 921, 2289
BseLI	CCNNNNNNNGG	5 cut(s)	1484, 1666, 2204, 2251, 3113
BseMI	GCAATG	4 cut(s)	560, 681, 2230, 2958
BseMII	CTCAG	5 cut(s)	25, 729, 2582, 2676, 2787
BseRI	GAGGAG	1 cut(s)	1191
BseXI	GCAGC	8 cut(s)	556, 977, 1657, 1845, 2035, 2390, 2607, 2682
BseYI	CCCAGC	3 cut(s)	730, 962, 2963
BshFI	GGCC	5 cut(s)	825, 1052, 1618, 2134, 2255
BshNI	GGYRCC	1 cut(s)	1787
BshVI	ATCGAT	1 cut(s)	1884
BsiHKCI	CYCGRG	1 cut(s)	2403
BsiSI	CCGG	3 cut(s)	174, 628, 1112
BslFI	GGGAC	1 cut(s)	1549
BslI	CCNNNNNNNGG	5 cut(s)	1484, 1666, 2204, 2251, 3113
BsmAI	GTCTC	6 cut(s)	1090, 1414, 1449, 1993, 2400, 2776
BsmFI	GGGAC	1 cut(s)	1549
BsmI	GAATGC	2 cut(s)	1238, 2590
BsnI	GGCC	5 cut(s)	825, 1052, 1618, 2134, 2255
Bso31I	GGTCTC	2 cut(s)	1993, 2776
BsoBI	CYCGRG	1 cut(s)	2403
Bsp13I	TCCGGA	1 cut(s)	1111
BspACI	CCGC	6 cut(s)	72, 895, 993, 2135, 2205, 2907
BspANI	GGCC	5 cut(s)	825, 1052, 1618, 2134, 2255
BspCNI	CTCAG	5 cut(s)	24, 730, 2581, 2675, 2786
BspDI	ATCGAT	1 cut(s)	1884
BspEI	TCCGGA	1 cut(s)	1111
BspHI	TCATGA	1 cut(s)	1345
BspLI	GGNNCC	4 cut(s)	1537, 1789, 2367, 2851
BspMAI	CTGCAG	2 cut(s)	2380, 2625
BspMI	ACCTGC	1 cut(s)	2383
BspPI	GGATC	7 cut(s)	557, 718, 1038, 1197, 1433, 2393, 2697
BspQI	GCTCTTC	3 cut(s)	120, 321, 3080
BspT107I	GGYRCC	1 cut(s)	1787
BspTNI	GGTCTC	2 cut(s)	1993, 2776
BsrBI	CCGCTC	2 cut(s)	895, 2205
BsrDI	GCAATG	4 cut(s)	560, 681, 2230, 2958
BsrFI	RCCGGY	1 cut(s)	173
BssAI	RCCGGY	1 cut(s)	173
BssECI	CCNNGG	4 cut(s)	273, 472, 2172, 3066
BssNI	GRCGYC	2 cut(s)	517, 2085
BssSI	CACGAG	1 cut(s)	2684
BssT1I	CCWWGG	1 cut(s)	2172
Bst2BI	CACGAG	1 cut(s)	2684
Bst2UI	CCWGG	1 cut(s)	1479
Bst6I	CTCTTC	5 cut(s)	120, 321, 1249, 2244, 3080
BstACI	GRCGYC	2 cut(s)	517, 2085
BstAPI	GCANNNNNTGC	2 cut(s)	550, 692
BstC8I	GCNNGC	7 cut(s)	175, 1042, 1755, 2464, 2490, 2836, 3099
BstDEI	CTNAG	8 cut(s)	11, 137, 210, 738, 2147, 2568, 2662, 2773
BstDSI	CCRYGG	2 cut(s)	472, 3066
BstEII	GGTNACC	1 cut(s)	1319
BstHHI	GCGC	1 cut(s)	3097
BstMAI	GTCTC	6 cut(s)	1090, 1414, 1449, 1993, 2400, 2776
BstMWI	GCNNNNNNNGC	5 cut(s)	550, 692, 1508, 2084, 2093
BstNI	CCWGG	1 cut(s)	1479
BstNSI	RCATGY	6 cut(s)	82, 1044, 1085, 1151, 1278, 2388
BstPI	GGTNACC	1 cut(s)	1319
BstSCI	CCNGG	2 cut(s)	626, 1477
BstSFI	CTRYAG	2 cut(s)	2376, 2621
BstV1I	GCAGC	8 cut(s)	556, 977, 1657, 1845, 2035, 2390, 2607, 2682
BstV2I	GAAGAC	1 cut(s)	891
BstX2I	RGATCY	5 cut(s)	19, 213, 562, 710, 1030
BstXI	CCANNNNNNTGG	1 cut(s)	2842
BstYI	RGATCY	5 cut(s)	19, 213, 562, 710, 1030
Bsu15I	ATCGAT	1 cut(s)	1884
BsuRI	GGCC	5 cut(s)	825, 1052, 1618, 2134, 2255
BsuTUI	ATCGAT	1 cut(s)	1884
BtgI	CCRYGG	2 cut(s)	472, 3066
BtsI	GCAGTG	1 cut(s)	3097
BtsIMutI	CAGTG	6 cut(s)	19, 813, 912, 1513, 1786, 3097
BveI	ACCTGC	1 cut(s)	2383
Cac8I	GCNNGC	7 cut(s)	175, 1042, 1755, 2464, 2490, 2836, 3099
CaiI	CAGNNNCTG	1 cut(s)	737
CciI	TCATGA	1 cut(s)	1345
CfoI	GCGC	1 cut(s)	3097
Cfr10I	RCCGGY	1 cut(s)	173
Cfr13I	GGNCC	4 cut(s)	630, 1290, 1536, 1616
ClaI	ATCGAT	1 cut(s)	1884
CseI	GACGC	3 cut(s)	506, 2093, 2876
Csp6I	GTAC	7 cut(s)	202, 870, 882, 2711, 2786, 2882, 2947
CviQI	GTAC	7 cut(s)	202, 870, 882, 2711, 2786, 2882, 2947
DdeI	CTNAG	8 cut(s)	11, 137, 210, 738, 2147, 2568, 2662, 2773
EaeI	YGGCCR	2 cut(s)	823, 2132
Eam1104I	CTCTTC	5 cut(s)	120, 321, 1249, 2244, 3080
EarI	CTCTTC	5 cut(s)	120, 321, 1249, 2244, 3080
Eco130I	CCWWGG	1 cut(s)	2172
Eco31I	GGTCTC	2 cut(s)	1993, 2776
Eco47I	GGWCC	3 cut(s)	630, 1290, 1536
Eco57I	CTGAAG	2 cut(s)	1272, 2268
Eco88I	CYCGRG	1 cut(s)	2403
Eco91I	GGTNACC	1 cut(s)	1319
EcoO65I	GGTNACC	1 cut(s)	1319
EcoRI	GAATTC	1 cut(s)	1942
EcoRII	CCWGG	1 cut(s)	1477
EcoT14I	CCWWGG	1 cut(s)	2172
EcoT22I	ATGCAT	1 cut(s)	697
ErhI	CCWWGG	1 cut(s)	2172
FalI	AAGNNNNNCTT	6 cut(s)	203, 235, 1245, 1277, 2497, 2529
FaqI	GGGAC	1 cut(s)	1549
FauNDI	CATATG	3 cut(s)	549, 1686, 1704
FbaI	TGATCA	2 cut(s)	601, 817
FblI	GTMKAC	1 cut(s)	478
FspBI	CTAG	4 cut(s)	947, 1067, 2154, 2750
GlaI	GCGC	1 cut(s)	3096
GsaI	CCCAGC	3 cut(s)	734, 966, 2967
GsuI	CTGGAG	2 cut(s)	888, 1914
HaeIII	GGCC	5 cut(s)	825, 1052, 1618, 2134, 2255
HapII	CCGG	3 cut(s)	174, 628, 1112
HgaI	GACGC	3 cut(s)	506, 2093, 2876
HhaI	GCGC	1 cut(s)	3097
Hin1I	GRCGYC	2 cut(s)	517, 2085
Hin6I	GCGC	1 cut(s)	3095
HinP1I	GCGC	1 cut(s)	3095
HindIII	AAGCTT	1 cut(s)	532
HpaII	CCGG	3 cut(s)	174, 628, 1112
HphI	GGTGA	5 cut(s)	1292, 1688, 2093, 2714, 3023
Hpy166II	GTNNAC	5 cut(s)	479, 1603, 2713, 2761, 2947
Hpy8I	GTNNAC	5 cut(s)	479, 1603, 2713, 2761, 2947
HpyAV	CCTTC	9 cut(s)	50, 415, 633, 1247, 1334, 2013, 2533, 2924, 3038
HpyCH4IV	ACGT	1 cut(s)	2804
HpyF10VI	GCNNNNNNNGC	5 cut(s)	550, 692, 1508, 2084, 2093
HpyF3I	CTNAG	8 cut(s)	11, 137, 210, 738, 2147, 2568, 2662, 2773
HpySE526I	ACGT	1 cut(s)	2804
Hsp92I	GRCGYC	2 cut(s)	517, 2085
HspAI	GCGC	1 cut(s)	3095
Kpn2I	TCCGGA	1 cut(s)	1111
KroI	GCCGGC	1 cut(s)	173
KroNI	GCCGGC	1 cut(s)	175
Ksp22I	TGATCA	2 cut(s)	601, 817
LguI	GCTCTTC	3 cut(s)	120, 321, 3080
LmnI	GCTCC	4 cut(s)	1037, 1357, 1480, 2371
Lsp1109I	GCAGC	8 cut(s)	556, 977, 1657, 1845, 2035, 2390, 2607, 2682
MaeI	CTAG	4 cut(s)	947, 1067, 2154, 2750
MaeII	ACGT	1 cut(s)	2804
MbiI	CCGCTC	2 cut(s)	895, 2205
MflI	RGATCY	5 cut(s)	19, 213, 562, 710, 1030
MlsI	TGGCCA	1 cut(s)	825
MluNI	TGGCCA	1 cut(s)	825
MlyI	GAGTC	7 cut(s)	340, 392, 1117, 2517, 2654, 2713, 2994
MmeI	TCCRAC	3 cut(s)	674, 931, 2977
Mox20I	TGGCCA	1 cut(s)	825
Mph1103I	ATGCAT	1 cut(s)	697
MroI	TCCGGA	1 cut(s)	1111
MroNI	GCCGGC	1 cut(s)	173
MscI	TGGCCA	1 cut(s)	825
MslI	CAYNNNNRTG	5 cut(s)	609, 1218, 1298, 1496, 2447
Msp20I	TGGCCA	1 cut(s)	825
MspA1I	CMGCKG	2 cut(s)	2215, 2695
MspI	CCGG	3 cut(s)	174, 628, 1112
MspR9I	CCNGG	2 cut(s)	628, 1479
Mva1269I	GAATGC	2 cut(s)	1238, 2590
MvaI	CCWGG	1 cut(s)	1479
MwoI	GCNNNNNNNGC	5 cut(s)	550, 692, 1508, 2084, 2093
NaeI	GCCGGC	1 cut(s)	175
NciI	CCSGG	1 cut(s)	628
NdeI	CATATG	3 cut(s)	549, 1686, 1704
NgoMIV	GCCGGC	1 cut(s)	173
NlaIV	GGNNCC	4 cut(s)	1537, 1789, 2367, 2851
NmeAIII	GCCGAG	1 cut(s)	298
NmuCI	GTSAC	6 cut(s)	384, 611, 2081, 2476, 2495, 2509
NsiI	ATGCAT	1 cut(s)	697
NspI	RCATGY	6 cut(s)	82, 1044, 1085, 1151, 1278, 2388
PaeI	GCATGC	1 cut(s)	1044
PaeR7I	CTCGAG	1 cut(s)	2403
PagI	TCATGA	1 cut(s)	1345
PaqCI	CACCTGC	1 cut(s)	2383
PciI	ACATGT	3 cut(s)	1081, 1147, 1274
PciSI	GCTCTTC	3 cut(s)	120, 321, 3080
PcsI	WCGNNNNNNNCGW	1 cut(s)	501
PctI	GAATGC	2 cut(s)	1238, 2590
PdiI	GCCGGC	1 cut(s)	175
PflMI	CCANNNNNTGG	1 cut(s)	1666
PfoI	TCCNGGA	1 cut(s)	626
PleI	GAGTC	7 cut(s)	340, 391, 1116, 2516, 2654, 2713, 2994
PpsI	GAGTC	7 cut(s)	340, 391, 1116, 2516, 2654, 2713, 2994
PscI	ACATGT	3 cut(s)	1081, 1147, 1274
PsiI	TTATAA	1 cut(s)	63
Psp1406I	AACGTT	1 cut(s)	2804
Psp6I	CCWGG	1 cut(s)	1477
PspEI	GGTNACC	1 cut(s)	1319
PspFI	CCCAGC	3 cut(s)	730, 962, 2963
PspGI	CCWGG	1 cut(s)	1477
PspN4I	GGNNCC	4 cut(s)	1537, 1789, 2367, 2851
PspPI	GGNCC	4 cut(s)	630, 1290, 1536, 1616
PstI	CTGCAG	2 cut(s)	2380, 2625
PstNI	CAGNNNCTG	1 cut(s)	737
PsuI	RGATCY	5 cut(s)	19, 213, 562, 710, 1030
PvuII	CAGCTG	2 cut(s)	2215, 2695
RsaI	GTAC	7 cut(s)	203, 871, 883, 2712, 2787, 2883, 2948
RsaNI	GTAC	7 cut(s)	202, 870, 882, 2711, 2786, 2882, 2947
RseI	CAYNNNNRTG	5 cut(s)	609, 1218, 1298, 1496, 2447
SapI	GCTCTTC	3 cut(s)	120, 321, 3080
Sau96I	GGNCC	4 cut(s)	630, 1290, 1536, 1616
ScaI	AGTACT	2 cut(s)	203, 871
SchI	GAGTC	7 cut(s)	340, 392, 1117, 2517, 2654, 2713, 2994
ScrFI	CCNGG	2 cut(s)	628, 1479
SfcI	CTRYAG	2 cut(s)	2376, 2621
Sfr274I	CTCGAG	1 cut(s)	2403
SinI	GGWCC	3 cut(s)	630, 1290, 1536
SlaI	CTCGAG	1 cut(s)	2403
SmiMI	CAYNNNNRTG	5 cut(s)	609, 1218, 1298, 1496, 2447
SmlI	CTYRAG	3 cut(s)	83, 2403, 3026
SmoI	CTYRAG	3 cut(s)	83, 2403, 3026
SphI	GCATGC	1 cut(s)	1044
SsiI	CCGC	6 cut(s)	72, 895, 993, 2135, 2205, 2907
SspI	AATATT	1 cut(s)	1197
SspMI	CTAG	4 cut(s)	947, 1067, 2154, 2750
StyD4I	CCNGG	2 cut(s)	626, 1477
StyI	CCWWGG	1 cut(s)	2172
TaiI	ACGT	1 cut(s)	2807
TaqI	TCGA	6 cut(s)	189, 206, 1046, 1644, 1884, 2404
TatI	WGTACW	4 cut(s)	201, 869, 881, 2881
TauI	GCSGC	3 cut(s)	74, 2137, 2208
TscAI	CASTG	6 cut(s)	19, 820, 912, 1513, 1786, 3097
TseFI	GTSAC	6 cut(s)	384, 611, 2081, 2476, 2495, 2509
TseI	GCWGC	8 cut(s)	544, 990, 1670, 1858, 2023, 2378, 2620, 2695
Tsp45I	GTSAC	6 cut(s)	384, 611, 2081, 2476, 2495, 2509
TspGWI	ACGGA	2 cut(s)	461, 1986
TspRI	CASTG	6 cut(s)	19, 820, 912, 1513, 1786, 3097
Van91I	CCANNNNNTGG	1 cut(s)	1666
VpaK11BI	GGWCC	3 cut(s)	630, 1290, 1536
XapI	RAATTY	4 cut(s)	485, 1694, 1942, 3079
XceI	RCATGY	6 cut(s)	82, 1044, 1085, 1151, 1278, 2388
XhoI	CTCGAG	1 cut(s)	2403
XmiI	GTMKAC	1 cut(s)	478
XspI	CTAG	4 cut(s)	947, 1067, 2154, 2750
ZrmI	AGTACT	2 cut(s)	203, 871
Zsp2I	ATGCAT	1 cut(s)	697

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.