Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0017445

Physical Location & Seq

Genomic Coordinates Forward (+)

10988443 .. 10997275

Gene Structure 8833 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00170560.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3600 bp

ATGGATCTGAACCCGTCCAGCTTCCCCATCCTTTCCTACGTCATGGACCGCCTTTCCACTGTAGGATCCAAGTCCACTATCGACGACATCTCCGACCTCGAGTCCATTCAAATCGACAATGGCCAGCTCCAGTTGATCAAACCCCAGCAACCATCCTCATCCTCCTCAGGCCTCCTCAACCAGATGCCCCACCTCATCACCGCCATGACTCGCGCTGTCTCCAACGTCGCCCAGACCCACTCCGTCCTCAACGCCCTCGGCGACCGTCCTGACCACGAGGCCGGTGGGATTGTGAGCAAGCTATGGCCAACTGCAGCCTATTTTGTGTTTCTTCCATTTGATATGAATGGGGGTGAGGCTACTGGTAGAGAAGTAGTTGATGTTTCAGGTGTTGAAGTGGGAAAGGTGTTAGTAGAACTTAAAGAGGGAAGCATGCTTCCGCAAGAAGAGTCAGTTGTAGCTTTGAGTAGGGGTATTTTGGAAAATTTTCCTGTGAAAAGGTTCCAGACTCTCTACCTTGGGCATTGTGGGATGTCTAATCTCAGTGAAGATCTTGTGTTCTTGGTCTTAAAGTATTTGGATGAGGAAGGTTATAAGGAAGCCGCCCACATGCTTGAGCGTGAAAGTGGGCTTTACTTTGACATGAATTATTTTGAAGAGCTGGTGCTTGGTGGAAAATGGGATGAGGCAGAGAAATACCTCACCGGCTTCACAAAGCTCGATGACAATAAGTACTCGATTAAGATCTACTTTGAAATCAGGAAGCAACATTACTTTGAGGCACTTGACAAAAAATGTCGTGCCGAGGCTTTAGATGTCCTCACAAATGATCTACAAGTTTTTGCTAAAGGTAGTGAAGAGCTATTCAAAGAAATGACTCAACTTATGACATTTGAGAATATAAGGGAACATGAGTCACTCTCTATGTATAGAGATACAAAGTCTGCAAGGAAGGTTATGATGAAAGAAATCAGGAGGGTAATTGAGGCAAATCCAATTTTCCGTGGTAGACTGGAATTTCCTATCCATCTGCAATGTGAAGATCCTCATCCAAATCCAGCCATTGACACCCTTTTTATTGATCATGTTTGTGACCCACAGCCTTCCCGGACCACATCATATCAATCTCCAACATGTTCTGTGAAGTCAAGTGTATCTACCATGGCACAATATGCATCGTGTATTGGAGGATCTTTCCCTGCAAAGAACCCAGCTACTGAGGTTATTGAGGATTCTGATGTTTTATCCAAAAGGATGCCCATTGTAACTGGGGATGAGGTAACATCAACTATCACTGATCATGGCCAAAATCAGATCTCGGAACTTGCCATATCCAAAAATCTTCCCAGTACTGTTGTCCGTACATTGAATGAGGGGTCTTCTCCAGTGAGCATGGATTTTCATCCCGTTTGGCTTACTGTTCTTCTAGTTGGAACTTCTGCTGGGGAAATAGGACTGTGGGATATTAGCTGCGGTGGAAAAATGGTTGCCAGAAACTTTAGTGTTTGGGATCTTGGAGCATGCTCGATGGCCTTAAAGCTAGCCCTAGAAAAAGATTCACATGTCTCTGTTAATCGTGTGTTATGGAGTCCGGATGGTTTCTTATTTGGGGTTGCATATTCAAAACATGTTGTGCAATTATATTCTTATATTGGAGGAGATGAACTGGATCAAATATTGGAAATTGATGCTCATTTGGGTGGTGTAAATGATTTAGCATTCTCTTCTCCTGAAGGGGAACTTTTGGTCATAACATGTGGTGATGACAGGACCATCAAGGTGTGGGATGTGGTTAGTGGTAACCGATGCTTTACTTTTGAAGGTCATGATGCTCCGGTTTATTCCATCTGTCCTCATATCAAAGACAATATCCCTTTTATCTTTTCAGTCTCAACAGTTGGCAGGATCAAAGTGTGGTTATATGACAATTTAGGAGACAGAATTGATTATGATGCTCCTGGTCTTGGTTGCACCAAAATGGCTTACAGTGCTGATGAGAGGCTTTTTTCATGTGGGACCAGTAAAGGAGGGGAATCATATCTTGTTGAATGGGATGATACTGTTGGTTGTTTCAAAAGAGTTTACAAAGGACTTGGGCCAAACCCCTTGTCTGCCGTGCAATTCGATATAACCAGGAACCAGCTTTTGGCTGCTGGTGATGACCATATGGTTAAATTTTGGCATATGGACAACACTCAACTTTTGAAAACCATTGATGCTGATGGTGGATTGCCAGCAAATCCACATATTCGCTTCAACAGTGATGGCACACTATTAGCTGTTTCTTCAAGTGGAAACCGAATCAAGATACTGGCAAATGCTAATGGTCTTGATTTGCTGCAAGGATTTGGGAATGATTCTATCGATGCCTCTGGAGTTGAATCTGAAACTTCAAGAAAGAATGGGAATGTGGCAGAAGTGAATTCAAAAACAACAGGAGAACCCACGTATGGAGAAAAGGCATGGGAAACTATTGAGAGACCTTCTCAATGCCAATCTTTGCAGCTATGTGCTGATGTCAAAAGAACCAAGATATCCAAGCTGATCTACACCAATGCCGGTGACGCCATTTTGGCATTAACATCAAATGCCATTCATCTACTCTGGAAATGGCCGCAGAATGATCTTAGTTCTAGTGGAAAGGCAACTACCAAGGTTCAACCTCTTTTATGGCGACCAACGAGTGGCTTACAGCTGATGAGCAATGACCTTTCCAGCACCAACCCTGAAGAGGCCATGCCATGCTTTGCTTTGTCCAAGAATGATTCATATCTTATGTCAGCATCAGGAGGGATGATCTCCCTTTTTAATGTTATATCGTTTAAGACAATGAGGAAAGTCATGGCTCTACCACCTGCAGCAACATGCCTTGCTTTTGATCCTCGAGACAACAATGTAGTTGCTATTGGCATGGATGATTCCACAATTATGATATATAATGCTCGCTTGGATAAGGTCACAAATAGGCTTGCTGGTCACACCAAAAGAGTCACTTCCCTTGCCTTCTCCACCTCTATGAAATCACTTGTTTCTTCTGGTGCTGATGCTCAGATTTTTGTGTGGAATGCTGATGAGTGGAAAATACAGAGAAGCCAAGTGCTGCAGATTCCAGATTGGAACAAATTGAGAGCATTATCAGACTCAGATACCCATGTCCAGGTTCACGAGGATCAGCTTCATTTCCTAATTGTACACAAGACTCACCTTGCAATATACGAAATGAAAGAACTAGGATGTGTAAACGAGTGGGTCTCAGATTCTTCTGTACCAATTTCCCATGCAACTTTCTCATGTGATAGCCAGATGGTATATGCCAGCTTTGTGGATGGAACTATAGGCATATTTGACGCTTCTGTTCTTGTACTATGCTGTCTAATCAAGCCCTCCGCTTACCTTCCTACTGATATAAGTTATTCTGTGTACCCAGTTGTCATTGCTGGGCATCCACAAAAACCAACCCAGTTTGCCGTTGGACTCTCTAATGGTGAAGTTCATGTGCTTGAGCCGTTAGAATCAGAAGGCAAATGGGGTATATTGCCAACGGTTAAAAGGAATTTGGAGAGCAGTGCGCCTGCAAAATCTACCTTTTGGGGCTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010051 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048523 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0090421 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1199

Amino Acids

132.2

Weight (kDa)

5.18

Isoelectric Point (pI)

37.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	182 - 210	5.8e-06	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	215 - 338	5.8e-53	TPR1-like, CTLH-containing domain
zf_topless	PF21359	343 - 364	6.4e-06	TOPLESS, zinc finger domain
Beta-prop_WDR3_2nd	PF25172	421 - 661	9.1e-06	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	499 - 616	1.3e-11	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	499 - 641	4.4e-14	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	522 - 640	5.1e-13	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	537 - 642	2.5e-08	WDHD1 first WD40 domain
WD40	PF00400	559 - 596	8.6e-07	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	659 - 776	2.3e-07	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	902 - 1118	1.5e-16	WDHD1 first WD40 domain
WDR55	PF24796	903 - 1023	1.1e-06	WDR55
Beta-prop_EML_2	PF23414	906 - 1042	2.3e-10	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	907 - 1027	5.6e-11	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	911 - 1117	1.3e-18	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	915 - 1115	7.3e-14	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	916 - 1027	2.5e-11	THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	926 - 1028	3.1e-10	WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st	PF25173	926 - 1023	2.7e-14	WDR3 first beta-propeller domain
Beta-prop_IFT140_1st	PF23383	927 - 1034	1.7e-07	IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	929 - 1023	2.3e-09	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR19_1st	PF23389	929 - 1076	3.9e-07	WDR19 first beta-propeller
WD40_Prp19	PF24814	951 - 1117	1.9e-16	Prp19 WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	958 - 1022	2e-06	MABP1/WDR62 second WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017445)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr6g0297391
rosa_laevigata	RLG00000011603
rosa_multiflora	Rmu_sc0004989.1_g000003
rosa_roxburghii	Rroxscaffold_7G00170560
rosa_rugosa	Rorug06G0271300 Rorug06G0271400 Rorug06G0271500 Rorug06G0271600
rosa_samantha	Rh6AG382800 Rh6BG391100 Rh6CG396000 Rh6DG383300
rosa_wichuraiana	Rw6G033340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	594
AarI	CACCTGC	1 cut(s)	2863
Acc36I	ACCTGC	1 cut(s)	2863
AccB7I	CCANNNNNTGG	2 cut(s)	2146, 2684
AccI	GTMKAC	1 cut(s)	1009
AccII	CGCG	1 cut(s)	213
AccIII	TCCGGA	1 cut(s)	1591
AciI	CCGC	7 cut(s)	49, 201, 440, 603, 1473, 2615, 3387
AcoI	YGGCCR	4 cut(s)	121, 305, 1303, 2612
AcsI	RAATTY	5 cut(s)	484, 1016, 2174, 2422, 3553
AcuI	CTGAAG	2 cut(s)	1752, 2748
AcyI	GRCGYC	1 cut(s)	2565
AfaI	GTAC	7 cut(s)	734, 1351, 1363, 3192, 3267, 3363, 3422
AfiI	CCNNNNNNNGG	6 cut(s)	1964, 2146, 2450, 2684, 2731, 3157
AflIII	ACRYGT	4 cut(s)	1133, 1561, 1627, 1754
AhdI	GACNNNNNGTC	1 cut(s)	100
AjnI	CCWGG	3 cut(s)	1957, 2132, 3156
AjuI	GAANNNNNNNTTGG	2 cut(s)	2129, 2161
Alw26I	GTCTC	7 cut(s)	223, 1570, 1894, 1929, 2473, 2880, 3256
AlwNI	CAGNNNCTG	1 cut(s)	1217
Ama87I	CYCGRG	2 cut(s)	98, 2883
Aor13HI	TCCGGA	1 cut(s)	1591
AoxI	GGCC	9 cut(s)	121, 169, 279, 305, 1303, 1530, 2096, 2612, 2733
ApeKI	GCWGC	7 cut(s)	314, 1470, 2150, 2338, 2503, 2858, 3100
ApoI	RAATTY	5 cut(s)	484, 1016, 2174, 2422, 3553
ArsI	GACNNNNNNTTYG	2 cut(s)	2093, 2125
Asp700I	GAANNNNTTC	2 cut(s)	486, 500
AspLEI	GCGC	2 cut(s)	215, 3571
AspS9I	GGNCC	5 cut(s)	46, 1110, 1770, 2016, 2096
AsuC2I	CCSGG	1 cut(s)	1108
AsuHPI	GGTGA	8 cut(s)	190, 365, 694, 1772, 2168, 2573, 3194, 3497
AsuNHI	GCTAGC	1 cut(s)	1540
AvaI	CYCGRG	2 cut(s)	98, 2883
AvaII	GGWCC	4 cut(s)	46, 1110, 1770, 2016
AxyI	CCTNAGG	1 cut(s)	166
BaeI	ACNNNNGTAYC	2 cut(s)	3138, 3171
BalI	TGGCCA	3 cut(s)	123, 307, 1305
BamHI	GGATCC	1 cut(s)	65
BarI	GAAGNNNNNNTAC	2 cut(s)	755, 787
BauI	CACGAG	2 cut(s)	275, 3164
BbsI	GAAGAC	1 cut(s)	1371
BbvI	GCAGC	7 cut(s)	326, 1457, 2137, 2325, 2515, 2870, 3087
BceAI	ACGGC	3 cut(s)	2099, 3452, 3490
BciT130I	CCWGG	3 cut(s)	1959, 2134, 3158
BclI	TGATCA	3 cut(s)	135, 1081, 1297
BcnI	CCSGG	1 cut(s)	1108
BcoDI	GTCTC	7 cut(s)	223, 1570, 1894, 1929, 2473, 2880, 3256
BfaI	CTAG	6 cut(s)	1427, 1541, 1547, 2634, 3230, 3598
BfmI	CTRYAG	5 cut(s)	60, 312, 2856, 3101, 3333
BfuAI	ACCTGC	1 cut(s)	2863
BglI	GCCNNNNNGGC	1 cut(s)	2573
BglII	AGATCT	3 cut(s)	550, 744, 1314
BisI	GCNGC	9 cut(s)	315, 603, 1471, 2151, 2339, 2504, 2615, 2859, 3101
BlsI	GCNGC	9 cut(s)	316, 604, 1472, 2152, 2340, 2505, 2616, 2860, 3102
BmcAI	AGTACT	2 cut(s)	734, 1351
Bme1390I	CCNGG	4 cut(s)	1108, 1959, 2134, 3158
Bme18I	GGWCC	4 cut(s)	46, 1110, 1770, 2016
BmeRI	GACNNNNNGTC	1 cut(s)	100
BmeT110I	CYCGRG	2 cut(s)	98, 2883
BmgT120I	GGNCC	5 cut(s)	46, 1110, 1770, 2016, 2096
BmiI	GGNNCC	4 cut(s)	67, 503, 2017, 2138
BmrFI	CCNGG	4 cut(s)	1108, 1959, 2134, 3158
BmrI	ACTGGG	4 cut(s)	1278, 1341, 3419, 3454
BmtI	GCTAGC	1 cut(s)	1544
BmuI	ACTGGG	4 cut(s)	1278, 1341, 3419, 3454
BpiI	GAAGAC	1 cut(s)	1371
BplI	GAGNNNNNCTC	6 cut(s)	905, 937, 2470, 2502, 3236, 3268
BpmI	CTGGAG	3 cut(s)	113, 1368, 2394
BpuEI	CTTGAG	2 cut(s)	635, 3521
BpuMI	CCSGG	1 cut(s)	1108
Bsa29I	ATCGAT	1 cut(s)	2364
BsaAI	YACGTR	1 cut(s)	2448
BsaBI	GATNNNNATC	4 cut(s)	743, 1047, 1401, 2769
BsaHI	GRCGYC	1 cut(s)	2565
BsaI	GGTCTC	2 cut(s)	2473, 3256
BsaJI	CCNNGG	6 cut(s)	256, 517, 804, 1003, 1161, 2652
BsaWI	WCCGGW	2 cut(s)	1591, 1834
BsaXI	ACNNNNNCTCC	2 cut(s)	74, 104
Bsc4I	CCNNNNNNNGG	6 cut(s)	1964, 2146, 2450, 2684, 2731, 3157
Bse118I	RCCGGY	3 cut(s)	281, 704, 2558
Bse21I	CCTNAGG	1 cut(s)	166
Bse3DI	GCAATG	3 cut(s)	1040, 2710, 3432
Bse8I	GATNNNNATC	4 cut(s)	743, 1047, 1401, 2769
BseAI	TCCGGA	1 cut(s)	1591
BseBI	CCWGG	3 cut(s)	1959, 2134, 3158
BseCI	ATCGAT	1 cut(s)	2364
BseDI	CCNNGG	6 cut(s)	256, 517, 804, 1003, 1161, 2652
BseJI	GATNNNNATC	4 cut(s)	743, 1047, 1401, 2769
BseLI	CCNNNNNNNGG	6 cut(s)	1964, 2146, 2450, 2684, 2731, 3157
BseMI	GCAATG	3 cut(s)	1040, 2710, 3432
BseMII	CTCAG	6 cut(s)	180, 556, 1209, 3062, 3156, 3267
BseRI	GAGGAG	3 cut(s)	154, 164, 1671
BseXI	GCAGC	7 cut(s)	326, 1457, 2137, 2325, 2515, 2870, 3087
BseYI	CCCAGC	4 cut(s)	144, 1210, 1442, 3437
Bsh1236I	CGCG	1 cut(s)	213
Bsh1285I	CGRYCG	1 cut(s)	265
BshFI	GGCC	9 cut(s)	123, 171, 281, 307, 1305, 1532, 2098, 2614, 2735
BshVI	ATCGAT	1 cut(s)	2364
BsiEI	CGRYCG	1 cut(s)	265
BsiHKCI	CYCGRG	2 cut(s)	98, 2883
BsiSI	CCGG	6 cut(s)	282, 705, 1108, 1592, 1835, 2559
BslFI	GGGAC	1 cut(s)	2029
BslI	CCNNNNNNNGG	6 cut(s)	1964, 2146, 2450, 2684, 2731, 3157
BsmAI	GTCTC	7 cut(s)	223, 1570, 1894, 1929, 2473, 2880, 3256
BsmFI	GGGAC	1 cut(s)	2029
BsmI	GAATGC	2 cut(s)	1718, 3070
BsnI	GGCC	9 cut(s)	123, 171, 281, 307, 1305, 1532, 2098, 2614, 2735
Bso31I	GGTCTC	2 cut(s)	2473, 3256
BsoBI	CYCGRG	2 cut(s)	98, 2883
Bsp13I	TCCGGA	1 cut(s)	1591
Bsp1407I	TGTACA	1 cut(s)	3190
Bsp19I	CCATGG	1 cut(s)	1161
BspACI	CCGC	7 cut(s)	49, 201, 440, 603, 1473, 2615, 3387
BspANI	GGCC	9 cut(s)	123, 171, 281, 307, 1305, 1532, 2098, 2614, 2735
BspCNI	CTCAG	6 cut(s)	179, 555, 1210, 3061, 3155, 3266
BspDI	ATCGAT	1 cut(s)	2364
BspEI	TCCGGA	1 cut(s)	1591
BspFNI	CGCG	1 cut(s)	213
BspHI	TCATGA	1 cut(s)	1825
BspLI	GGNNCC	4 cut(s)	67, 503, 2017, 2138
BspMAI	CTGCAG	3 cut(s)	316, 2860, 3105
BspMI	ACCTGC	1 cut(s)	2863
BspOI	GCTAGC	1 cut(s)	1544
BspQI	GCTCTTC	2 cut(s)	651, 852
BspTNI	GGTCTC	2 cut(s)	2473, 3256
BsrDI	GCAATG	3 cut(s)	1040, 2710, 3432
BsrFI	RCCGGY	3 cut(s)	281, 704, 2558
BsrGI	TGTACA	1 cut(s)	3190
BssAI	RCCGGY	3 cut(s)	281, 704, 2558
BssECI	CCNNGG	6 cut(s)	256, 517, 804, 1003, 1161, 2652
BssNI	GRCGYC	1 cut(s)	2565
BssSI	CACGAG	2 cut(s)	275, 3164
BssT1I	CCWWGG	3 cut(s)	517, 1161, 2652
Bst2BI	CACGAG	2 cut(s)	275, 3164
Bst2UI	CCWGG	3 cut(s)	1959, 2134, 3158
Bst6I	CTCTTC	5 cut(s)	441, 651, 852, 1729, 2724
BstACI	GRCGYC	1 cut(s)	2565
BstAPI	GCANNNNNTGC	1 cut(s)	1172
BstAUI	TGTACA	1 cut(s)	3190
BstBAI	YACGTR	1 cut(s)	2448
BstDEI	CTNAG	7 cut(s)	166, 542, 1218, 2627, 3048, 3142, 3253
BstDSI	CCRYGG	2 cut(s)	1003, 1161
BstEII	GGTNACC	1 cut(s)	1799
BstFNI	CGCG	1 cut(s)	213
BstHHI	GCGC	2 cut(s)	215, 3571
BstMAI	GTCTC	7 cut(s)	223, 1570, 1894, 1929, 2473, 2880, 3256
BstMCI	CGRYCG	1 cut(s)	265
BstMWI	GCNNNNNNNGC	4 cut(s)	1172, 1988, 2564, 2573
BstNI	CCWGG	3 cut(s)	1959, 2134, 3158
BstNSI	RCATGY	8 cut(s)	436, 613, 1137, 1524, 1565, 1631, 1758, 2868
BstPI	GGTNACC	1 cut(s)	1799
BstSCI	CCNGG	4 cut(s)	1106, 1957, 2132, 3156
BstSFI	CTRYAG	5 cut(s)	60, 312, 2856, 3101, 3333
BstUI	CGCG	1 cut(s)	213
BstV1I	GCAGC	7 cut(s)	326, 1457, 2137, 2325, 2515, 2870, 3087
BstV2I	GAAGAC	1 cut(s)	1371
BstX2I	RGATCY	8 cut(s)	4, 65, 550, 744, 1042, 1190, 1314, 1510
BstXI	CCANNNNNNTGG	1 cut(s)	3322
BstYI	RGATCY	8 cut(s)	4, 65, 550, 744, 1042, 1190, 1314, 1510
Bsu15I	ATCGAT	1 cut(s)	2364
Bsu36I	CCTNAGG	1 cut(s)	166
BsuRI	GGCC	9 cut(s)	123, 171, 281, 307, 1305, 1532, 2098, 2614, 2735
BsuTUI	ATCGAT	1 cut(s)	2364
BtgI	CCRYGG	2 cut(s)	1003, 1161
BtsI	GCAGTG	1 cut(s)	3571
BtsIMutI	CAGTG	7 cut(s)	57, 550, 1293, 1392, 1993, 2266, 3571
BveI	ACCTGC	1 cut(s)	2863
CaiI	CAGNNNCTG	1 cut(s)	1217
CciI	TCATGA	1 cut(s)	1825
CfoI	GCGC	2 cut(s)	215, 3571
Cfr10I	RCCGGY	3 cut(s)	281, 704, 2558
Cfr13I	GGNCC	5 cut(s)	46, 1110, 1770, 2016, 2096
ClaI	ATCGAT	1 cut(s)	2364
CseI	GACGC	2 cut(s)	2573, 3356
Csp6I	GTAC	7 cut(s)	733, 1350, 1362, 3191, 3266, 3362, 3421
CviQI	GTAC	7 cut(s)	733, 1350, 1362, 3191, 3266, 3362, 3421
DdeI	CTNAG	7 cut(s)	166, 542, 1218, 2627, 3048, 3142, 3253
DriI	GACNNNNNGTC	1 cut(s)	100
EaeI	YGGCCR	4 cut(s)	121, 305, 1303, 2612
Eam1104I	CTCTTC	5 cut(s)	441, 651, 852, 1729, 2724
Eam1105I	GACNNNNNGTC	1 cut(s)	100
EarI	CTCTTC	5 cut(s)	441, 651, 852, 1729, 2724
Eco130I	CCWWGG	3 cut(s)	517, 1161, 2652
Eco147I	AGGCCT	1 cut(s)	171
Eco31I	GGTCTC	2 cut(s)	2473, 3256
Eco32I	GATATC	1 cut(s)	2535
Eco47I	GGWCC	4 cut(s)	46, 1110, 1770, 2016
Eco57I	CTGAAG	2 cut(s)	1752, 2748
Eco81I	CCTNAGG	1 cut(s)	166
Eco88I	CYCGRG	2 cut(s)	98, 2883
Eco91I	GGTNACC	1 cut(s)	1799
EcoO65I	GGTNACC	1 cut(s)	1799
EcoRI	GAATTC	1 cut(s)	2422
EcoRII	CCWGG	3 cut(s)	1957, 2132, 3156
EcoRV	GATATC	1 cut(s)	2535
EcoT14I	CCWWGG	3 cut(s)	517, 1161, 2652
EcoT22I	ATGCAT	1 cut(s)	1177
ErhI	CCWWGG	3 cut(s)	517, 1161, 2652
FalI	AAGNNNNNCTT	6 cut(s)	734, 766, 1725, 1757, 2977, 3009
FaqI	GGGAC	1 cut(s)	2029
FauNDI	CATATG	2 cut(s)	2166, 2184
FbaI	TGATCA	3 cut(s)	135, 1081, 1297
FblI	GTMKAC	1 cut(s)	1009
Fnu4HI	GCNGC	9 cut(s)	315, 603, 1471, 2151, 2339, 2504, 2615, 2859, 3101
Fsp4HI	GCNGC	9 cut(s)	315, 603, 1471, 2151, 2339, 2504, 2615, 2859, 3101
FspBI	CTAG	6 cut(s)	1427, 1541, 1547, 2634, 3230, 3598
GlaI	GCGC	2 cut(s)	214, 3570
GluI	GCNGC	9 cut(s)	315, 603, 1471, 2151, 2339, 2504, 2615, 2859, 3101
GsaI	CCCAGC	4 cut(s)	148, 1214, 1446, 3441
GsuI	CTGGAG	3 cut(s)	113, 1368, 2394
HaeIII	GGCC	9 cut(s)	123, 171, 281, 307, 1305, 1532, 2098, 2614, 2735
HapII	CCGG	6 cut(s)	282, 705, 1108, 1592, 1835, 2559
HgaI	GACGC	2 cut(s)	2573, 3356
HhaI	GCGC	2 cut(s)	215, 3571
Hin1I	GRCGYC	1 cut(s)	2565
Hin6I	GCGC	2 cut(s)	213, 3569
HinP1I	GCGC	2 cut(s)	213, 3569
HpaII	CCGG	6 cut(s)	282, 705, 1108, 1592, 1835, 2559
HphI	GGTGA	8 cut(s)	190, 365, 694, 1772, 2168, 2573, 3194, 3497
Hpy166II	GTNNAC	7 cut(s)	75, 1010, 2083, 3163, 3193, 3241, 3421
Hpy8I	GTNNAC	7 cut(s)	75, 1010, 2083, 3163, 3193, 3241, 3421
Hpy99I	CGWCG	2 cut(s)	86, 230
HpyAV	CCTTC	9 cut(s)	581, 946, 1113, 1727, 1814, 2493, 3013, 3404, 3512
HpyCH4IV	ACGT	3 cut(s)	39, 225, 2447
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1172, 1988, 2564, 2573
HpyF3I	CTNAG	7 cut(s)	166, 542, 1218, 2627, 3048, 3142, 3253
HpySE526I	ACGT	3 cut(s)	39, 225, 2447
Hsp92I	GRCGYC	1 cut(s)	2565
HspAI	GCGC	2 cut(s)	213, 3569
Kpn2I	TCCGGA	1 cut(s)	1591
Ksp22I	TGATCA	3 cut(s)	135, 1081, 1297
LguI	GCTCTTC	2 cut(s)	651, 852
LmnI	GCTCC	4 cut(s)	132, 1517, 1837, 1960
Lsp1109I	GCAGC	7 cut(s)	326, 1457, 2137, 2325, 2515, 2870, 3087
MaeI	CTAG	6 cut(s)	1427, 1541, 1547, 2634, 3230, 3598
MaeII	ACGT	3 cut(s)	39, 225, 2447
MaeIII	GTNAC	9 cut(s)	915, 1091, 1264, 1279, 1799, 2561, 2956, 2975, 2989
MflI	RGATCY	8 cut(s)	4, 65, 550, 744, 1042, 1190, 1314, 1510
MlsI	TGGCCA	3 cut(s)	123, 307, 1305
MluNI	TGGCCA	3 cut(s)	123, 307, 1305
MmeI	TCCRAC	5 cut(s)	117, 246, 1154, 1411, 3451
Mox20I	TGGCCA	3 cut(s)	123, 307, 1305
Mph1103I	ATGCAT	1 cut(s)	1177
MroI	TCCGGA	1 cut(s)	1591
MroXI	GAANNNNTTC	2 cut(s)	486, 500
MscI	TGGCCA	3 cut(s)	123, 307, 1305
MslI	CAYNNNNRTG	7 cut(s)	203, 1089, 1698, 1778, 1976, 2927, 3014
Msp20I	TGGCCA	3 cut(s)	123, 307, 1305
MspA1I	CMGCKG	1 cut(s)	2695
MspI	CCGG	6 cut(s)	282, 705, 1108, 1592, 1835, 2559
MspR9I	CCNGG	4 cut(s)	1108, 1959, 2134, 3158
Mva1269I	GAATGC	2 cut(s)	1718, 3070
MvaI	CCWGG	3 cut(s)	1959, 2134, 3158
MvnI	CGCG	1 cut(s)	213
MwoI	GCNNNNNNNGC	4 cut(s)	1172, 1988, 2564, 2573
NciI	CCSGG	1 cut(s)	1108
NcoI	CCATGG	1 cut(s)	1161
NdeI	CATATG	2 cut(s)	2166, 2184
NheI	GCTAGC	1 cut(s)	1540
NlaIV	GGNNCC	4 cut(s)	67, 503, 2017, 2138
NmeAIII	GCCGAG	2 cut(s)	237, 829
NmuCI	GTSAC	6 cut(s)	915, 1091, 2561, 2956, 2975, 2989
NsiI	ATGCAT	1 cut(s)	1177
NspI	RCATGY	8 cut(s)	436, 613, 1137, 1524, 1565, 1631, 1758, 2868
PaeI	GCATGC	2 cut(s)	436, 1524
PaeR7I	CTCGAG	2 cut(s)	98, 2883
PagI	TCATGA	1 cut(s)	1825
PaqCI	CACCTGC	1 cut(s)	2863
PceI	AGGCCT	1 cut(s)	171
PciI	ACATGT	4 cut(s)	1133, 1561, 1627, 1754
PciSI	GCTCTTC	2 cut(s)	651, 852
PcsI	WCGNNNNNNNCGW	2 cut(s)	90, 258
PctI	GAATGC	2 cut(s)	1718, 3070
PdmI	GAANNNNTTC	2 cut(s)	486, 500
PflMI	CCANNNNNTGG	2 cut(s)	2146, 2684
PfoI	TCCNGGA	1 cut(s)	1106
PkrI	GCNGC	9 cut(s)	316, 604, 1472, 2152, 2340, 2505, 2616, 2860, 3102
Ppu21I	YACGTR	1 cut(s)	2448
PscI	ACATGT	4 cut(s)	1133, 1561, 1627, 1754
PsiI	TTATAA	1 cut(s)	594
Psp6I	CCWGG	3 cut(s)	1957, 2132, 3156
PspEI	GGTNACC	1 cut(s)	1799
PspFI	CCCAGC	4 cut(s)	144, 1210, 1442, 3437
PspGI	CCWGG	3 cut(s)	1957, 2132, 3156
PspN4I	GGNNCC	4 cut(s)	67, 503, 2017, 2138
PspPI	GGNCC	5 cut(s)	46, 1110, 1770, 2016, 2096
PspXI	VCTCGAGB	1 cut(s)	98
PstI	CTGCAG	3 cut(s)	316, 2860, 3105
PstNI	CAGNNNCTG	1 cut(s)	1217
PsuI	RGATCY	8 cut(s)	4, 65, 550, 744, 1042, 1190, 1314, 1510
PvuII	CAGCTG	1 cut(s)	2695
RsaI	GTAC	7 cut(s)	734, 1351, 1363, 3192, 3267, 3363, 3422
RsaNI	GTAC	7 cut(s)	733, 1350, 1362, 3191, 3266, 3362, 3421
RseI	CAYNNNNRTG	7 cut(s)	203, 1089, 1698, 1778, 1976, 2927, 3014
SapI	GCTCTTC	2 cut(s)	651, 852
SatI	GCNGC	9 cut(s)	315, 603, 1471, 2151, 2339, 2504, 2615, 2859, 3101
Sau96I	GGNCC	5 cut(s)	46, 1110, 1770, 2016, 2096
ScaI	AGTACT	2 cut(s)	734, 1351
ScrFI	CCNGG	4 cut(s)	1108, 1959, 2134, 3158
SfcI	CTRYAG	5 cut(s)	60, 312, 2856, 3101, 3333
Sfr274I	CTCGAG	2 cut(s)	98, 2883
SinI	GGWCC	4 cut(s)	46, 1110, 1770, 2016
SlaI	CTCGAG	2 cut(s)	98, 2883
SmiMI	CAYNNNNRTG	7 cut(s)	203, 1089, 1698, 1778, 1976, 2927, 3014
SmlI	CTYRAG	4 cut(s)	98, 614, 2883, 3500
SmoI	CTYRAG	4 cut(s)	98, 614, 2883, 3500
SphI	GCATGC	2 cut(s)	436, 1524
SseBI	AGGCCT	1 cut(s)	171
SsiI	CCGC	7 cut(s)	49, 201, 440, 603, 1473, 2615, 3387
SspI	AATATT	1 cut(s)	1677
SspMI	CTAG	6 cut(s)	1427, 1541, 1547, 2634, 3230, 3598
StuI	AGGCCT	1 cut(s)	171
StyD4I	CCNGG	4 cut(s)	1106, 1957, 2132, 3156
StyI	CCWWGG	3 cut(s)	517, 1161, 2652
TaiI	ACGT	3 cut(s)	42, 228, 2450
TaqI	TCGA	9 cut(s)	81, 99, 114, 720, 737, 1526, 2124, 2364, 2884
TatI	WGTACW	4 cut(s)	732, 1349, 3190, 3361
TauI	GCSGC	2 cut(s)	605, 2617
TscAI	CASTG	7 cut(s)	64, 550, 1300, 1392, 1993, 2266, 3571
TseFI	GTSAC	6 cut(s)	915, 1091, 2561, 2956, 2975, 2989
TseI	GCWGC	7 cut(s)	314, 1470, 2150, 2338, 2503, 2858, 3100
Tsp45I	GTSAC	6 cut(s)	915, 1091, 2561, 2956, 2975, 2989
TspGWI	ACGGA	3 cut(s)	232, 992, 1349
TspRI	CASTG	7 cut(s)	64, 550, 1300, 1392, 1993, 2266, 3571
Van91I	CCANNNNNTGG	2 cut(s)	2146, 2684
VpaK11BI	GGWCC	4 cut(s)	46, 1110, 1770, 2016
XapI	RAATTY	5 cut(s)	484, 1016, 2174, 2422, 3553
XceI	RCATGY	8 cut(s)	436, 613, 1137, 1524, 1565, 1631, 1758, 2868
XcmI	CCANNNNNNNNNTGG	2 cut(s)	281, 3467
XhoI	CTCGAG	2 cut(s)	98, 2883
XmiI	GTMKAC	1 cut(s)	1009
XmnI	GAANNNNTTC	2 cut(s)	486, 500
XspI	CTAG	6 cut(s)	1427, 1541, 1547, 2634, 3230, 3598
ZrmI	AGTACT	2 cut(s)	734, 1351
Zsp2I	ATGCAT	1 cut(s)	1177

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.