RLG00000011632

At4g15545-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0019896
Physical Location & Seq
Reverse (-)
11194565 .. 11195141
577 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011632

Sequence Viewer

Length: 480 bp
ATGTCGCAAGGAAGCGGAAGCGTCGTCAGCGGAGGAGGGGGGCGGGATTTTCAATTGCCGGACGAGATCATGGCTGTGATTCCGAGCGACCGGTACGAGCAGCTGGATCTGGCGAGGAAAATCATGTCGATGGCGATAGCCGCTAGGGTTTCGAATCTCGTGGCAGAGGTCGGGAGGCTGAGGCAAAATCTGAGCGAGAAAGATAGGGTTATTTTTGACCTCGAGGAAAGAGCCTCGCGGCTCCAGCATGTTAACCACGATGCCGGCGTAACCCGAATTGCTGTAGACGCGGGTTGGGCTGCTGCAGGGGAGGAGGATGAGCAGGCCGGTTGTGTGCAGGGGAAAAGGCCAGTGTGGCGTAACCCGAATTGCTCCAATCCGATATCTGTGCGGGTTCCGGTTCGAGTTTTCGAAGTTGCTTGGATTGGGGTGTGGAAGATCTCGCCCGAGTGTGAGGATTTGAGGTTTGTGATGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

17.56

Weight (kDa)

5.45

Isoelectric Point (pI)

48.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019896)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0099961
rosa_laevigata RLG00000011632
rosa_multiflora Rmu_co8036340.1_g000001 Rmu_sc0006675.1_g000006 Rmu_sc0007877.1_g000022
rosa_samantha Rh2AG133400 Rh6AG194200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 221
AccI GTMKAC 1 cut(s) 285
AccII CGCG 2 cut(s) 238, 290
AciI CCGC 7 cut(s) 15, 30, 43, 141, 238, 290, 391
AclWI GGATC 1 cut(s) 114
AfaI GTAC 1 cut(s) 95
AgeI ACCGGT 1 cut(s) 90
AgsI TTSAA 1 cut(s) 53
AluBI AGCT 1 cut(s) 103
AluI AGCT 1 cut(s) 103
AlwI GGATC 1 cut(s) 114
Ama87I CYCGRG 2 cut(s) 221, 446
AoxI GGCC 2 cut(s) 324, 347
ApeKI GCWGC 3 cut(s) 100, 299, 302
AsiGI ACCGGT 1 cut(s) 90
AsuII TTCGAA 2 cut(s) 152, 411
AvaI CYCGRG 2 cut(s) 221, 446
BauI CACGAG 1 cut(s) 158
BbvCI CCTCAGC 1 cut(s) 179
BbvI GCAGC 3 cut(s) 112, 286, 289
BccI CCATC 1 cut(s) 124
BcgI CGANNNNNNTGC 2 cut(s) 370, 404
BfaI CTAG 1 cut(s) 144
BfmI CTRYAG 2 cut(s) 282, 303
BglI GCCNNNNNGGC 1 cut(s) 355
BglII AGATCT 1 cut(s) 438
BisI GCNGC 5 cut(s) 101, 141, 239, 300, 303
BlsI GCNGC 5 cut(s) 102, 142, 240, 301, 304
BmeT110I CYCGRG 2 cut(s) 221, 446
BmiI GGNNCC 2 cut(s) 242, 396
BmsI GCATC 1 cut(s) 250
BpmI CTGGAG 1 cut(s) 227
Bpu10I CCTNAGC 1 cut(s) 179
Bpu14I TTCGAA 2 cut(s) 152, 411
BsaWI WCCGGW 2 cut(s) 90, 397
Bse118I RCCGGY 3 cut(s) 90, 263, 326
Bse1I ACTGG 1 cut(s) 350
BseGI GGATG 1 cut(s) 322
BseMII CTCAG 2 cut(s) 170, 182
BseNI ACTGG 1 cut(s) 350
BseRI GAGGAG 2 cut(s) 48, 326
BseXI GCAGC 3 cut(s) 112, 286, 289
BsgI GTGCAG 1 cut(s) 356
Bsh1236I CGCG 2 cut(s) 238, 290
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 2 cut(s) 326, 349
BshTI ACCGGT 1 cut(s) 90
BsiEI CGRYCG 1 cut(s) 91
BsiHKCI CYCGRG 2 cut(s) 221, 446
BsiSI CCGG 5 cut(s) 59, 91, 264, 327, 398
BsnI GGCC 2 cut(s) 326, 349
BsoBI CYCGRG 2 cut(s) 221, 446
Bsp119I TTCGAA 2 cut(s) 152, 411
Bsp143I GATC 4 cut(s) 66, 106, 438, 474
BspACI CCGC 7 cut(s) 15, 30, 43, 141, 238, 290, 391
BspANI GGCC 2 cut(s) 326, 349
BspCNI CTCAG 2 cut(s) 171, 183
BspFNI CGCG 2 cut(s) 238, 290
BspLI GGNNCC 2 cut(s) 242, 396
BspMAI CTGCAG 1 cut(s) 307
BspPI GGATC 1 cut(s) 114
BspT104I TTCGAA 2 cut(s) 152, 411
BsrFI RCCGGY 3 cut(s) 90, 263, 326
BsrI ACTGG 1 cut(s) 350
BssAI RCCGGY 3 cut(s) 90, 263, 326
BssMI GATC 4 cut(s) 66, 106, 438, 474
BssSI CACGAG 1 cut(s) 158
Bst2BI CACGAG 1 cut(s) 158
BstBI TTCGAA 2 cut(s) 152, 411
BstC8I GCNNGC 2 cut(s) 265, 324
BstDEI CTNAG 2 cut(s) 179, 191
BstF5I GGATG 1 cut(s) 322
BstFNI CGCG 2 cut(s) 238, 290
BstKTI GATC 4 cut(s) 69, 109, 441, 477
BstMBI GATC 4 cut(s) 66, 106, 438, 474
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 6 cut(s) 27, 140, 244, 287, 296, 355
BstNSI RCATGY 1 cut(s) 251
BstSFI CTRYAG 2 cut(s) 282, 303
BstUI CGCG 2 cut(s) 238, 290
BstV1I GCAGC 3 cut(s) 112, 286, 289
BstX2I RGATCY 2 cut(s) 106, 438
BstYI RGATCY 2 cut(s) 106, 438
BsuRI GGCC 2 cut(s) 326, 349
BtsCI GGATG 1 cut(s) 322
BtsIMutI CAGTG 1 cut(s) 357
Cac8I GCNNGC 2 cut(s) 265, 324
Cfr10I RCCGGY 3 cut(s) 90, 263, 326
CseI GACGC 2 cut(s) 10, 296
Csp6I GTAC 1 cut(s) 94
CspAI ACCGGT 1 cut(s) 90
CviAII CATG 3 cut(s) 70, 124, 248
CviJI RGCY 9 cut(s) 74, 103, 140, 178, 233, 241, 299, 326, 349
CviKI_1 RGCY 9 cut(s) 74, 103, 140, 178, 233, 241, 299, 326, 349
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 2 cut(s) 179, 191
DpnI GATC 4 cut(s) 68, 108, 440, 476
DpnII GATC 4 cut(s) 66, 106, 438, 474
Eco32I GATATC 1 cut(s) 384
Eco88I CYCGRG 2 cut(s) 221, 446
EcoRV GATATC 1 cut(s) 384
FaeI CATG 3 cut(s) 73, 127, 251
FaiI YATR 3 cut(s) 71, 125, 249
FatI CATG 3 cut(s) 69, 123, 247
FauI CCCGC 3 cut(s) 36, 283, 384
FblI GTMKAC 1 cut(s) 285
Fnu4HI GCNGC 5 cut(s) 101, 141, 239, 300, 303
FokI GGATG 1 cut(s) 329
Fsp4HI GCNGC 5 cut(s) 101, 141, 239, 300, 303
FspBI CTAG 1 cut(s) 144
GluI GCNGC 5 cut(s) 101, 141, 239, 300, 303
GsuI CTGGAG 1 cut(s) 227
HaeIII GGCC 2 cut(s) 326, 349
HapII CCGG 5 cut(s) 59, 91, 264, 327, 398
HgaI GACGC 2 cut(s) 10, 296
Hin1II CATG 3 cut(s) 73, 127, 251
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 2 cut(s) 79, 154
HpaI GTTAAC 1 cut(s) 253
HpaII CCGG 5 cut(s) 59, 91, 264, 327, 398
Hpy166II GTNNAC 2 cut(s) 253, 286
Hpy188I TCNGA 4 cut(s) 84, 192, 381, 479
Hpy188III TCNNGA 1 cut(s) 172
Hpy8I GTNNAC 2 cut(s) 253, 286
Hpy99I CGWCG 1 cut(s) 26
HpyCH4V TGCA 2 cut(s) 305, 337
HpyF10VI GCNNNNNNNGC 6 cut(s) 27, 140, 244, 287, 296, 355
HpyF3I CTNAG 2 cut(s) 179, 191
Hsp92II CATG 3 cut(s) 73, 127, 251
KroI GCCGGC 1 cut(s) 263
KroNI GCCGGC 1 cut(s) 265
KspAI GTTAAC 1 cut(s) 253
Kzo9I GATC 4 cut(s) 66, 106, 438, 474
LmnI GCTCC 2 cut(s) 246, 377
Lsp1109I GCAGC 3 cut(s) 112, 286, 289
LweI GCATC 1 cut(s) 250
MaeI CTAG 1 cut(s) 144
MaeIII GTNAC 2 cut(s) 268, 359
MalI GATC 4 cut(s) 68, 108, 440, 476
MboI GATC 4 cut(s) 66, 106, 438, 474
MboII GAAGA 1 cut(s) 448
MfeI CAATTG 1 cut(s) 53
MflI RGATCY 2 cut(s) 106, 438
MluCI AATT 3 cut(s) 53, 276, 367
MroNI GCCGGC 1 cut(s) 263
MseI TTAA 1 cut(s) 252
MslI CAYNNNNRTG 2 cut(s) 74, 128
MspA1I CMGCKG 2 cut(s) 30, 103
MspI CCGG 5 cut(s) 59, 91, 264, 327, 398
MunI CAATTG 1 cut(s) 53
MvnI CGCG 2 cut(s) 238, 290
MwoI GCNNNNNNNGC 6 cut(s) 27, 140, 244, 287, 296, 355
NaeI GCCGGC 1 cut(s) 265
NdeII GATC 4 cut(s) 66, 106, 438, 474
NgoMIV GCCGGC 1 cut(s) 263
NlaIII CATG 3 cut(s) 73, 127, 251
NlaIV GGNNCC 2 cut(s) 242, 396
NspI RCATGY 1 cut(s) 251
NspV TTCGAA 2 cut(s) 152, 411
PaeR7I CTCGAG 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PdiI GCCGGC 1 cut(s) 265
PfeI GAWTC 2 cut(s) 79, 154
PinAI ACCGGT 1 cut(s) 90
PkrI GCNGC 5 cut(s) 102, 142, 240, 301, 304
PspN4I GGNNCC 2 cut(s) 242, 396
PspXI VCTCGAGB 1 cut(s) 221
PstI CTGCAG 1 cut(s) 307
PsuI RGATCY 2 cut(s) 106, 438
PvuII CAGCTG 1 cut(s) 103
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
RseI CAYNNNNRTG 2 cut(s) 74, 128
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 5 cut(s) 101, 141, 239, 300, 303
Sau3AI GATC 4 cut(s) 66, 106, 438, 474
SetI ASST 4 cut(s) 105, 171, 222, 467
SfaNI GCATC 1 cut(s) 250
SfcI CTRYAG 2 cut(s) 282, 303
Sfr274I CTCGAG 1 cut(s) 221
SfuI TTCGAA 2 cut(s) 152, 411
SlaI CTCGAG 1 cut(s) 221
SmiMI CAYNNNNRTG 2 cut(s) 74, 128
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
Sse9I AATT 3 cut(s) 53, 276, 367
SsiI CCGC 7 cut(s) 15, 30, 43, 141, 238, 290, 391
SspMI CTAG 1 cut(s) 144
TaqI TCGA 5 cut(s) 128, 152, 222, 403, 411
TasI AATT 3 cut(s) 53, 276, 367
TauI GCSGC 2 cut(s) 143, 241
TfiI GAWTC 2 cut(s) 79, 154
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 1 cut(s) 357
TseI GCWGC 3 cut(s) 100, 299, 302
TspRI CASTG 1 cut(s) 357
XceI RCATGY 1 cut(s) 251
XhoI CTCGAG 1 cut(s) 221
XmiI GTMKAC 1 cut(s) 285
XspI CTAG 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.