Rmu_sc0006675.1_g000006

At4g15545-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006675.1
OG0019896
Physical Location & Seq
Reverse (-)
28397 .. 29092
696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006675.1_g000006.1.cds

Sequence Viewer

Length: 696 bp
atgatctatgaccacaccgaaaccccttctcaatctcttttttctcagtctttccctcatcttcttcctccacctccaccaccacttgatctccttgatccaccttcacgccgaaaacctccgacgccattttttcattgtgcccaaagcgatttagagacgactaccaaaccgaaccaccacaaccaccgtaggaggaggaggagcgatcggctatgtcgcaaggaagtggaagcgtcgtcggcgaaggagggggggcgggattttcaattgccgaacgagatcatggtggtgattccgagcgacccgtatgagcagctggatctggcgaggaagatcacgtcgatggtgatagccgctagggtttcgaatcttgaggcggaggtcgggaggctgaggcagaagctaagcgagaaagatagggttatttttgacctcgaggaaagagcctcgaggctccagcatgttaaccatgacgccgacgtaacccgaattgctgtagatgctggtgggctgctgcaagggaggaggatgcgcaggccagttgtgtgcaggggaaagggccagtatgggttggcagcaaagcagcaagggttgcaagggctgggtggtggtgggatgtgcgcgcagaggctaggcgagaatgaggatctgggctcctccgtcgccggcgtaatgcgaattgctctaatctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

231

Amino Acids

25.75

Weight (kDa)

9.35

Isoelectric Point (pI)

64.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019896)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0099961
rosa_laevigata RLG00000011632
rosa_multiflora Rmu_co8036340.1_g000001 Rmu_sc0006675.1_g000006 Rmu_sc0007877.1_g000022
rosa_samantha Rh2AG133400 Rh6AG194200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 2 cut(s) 437, 451
Acc16I TGCGCA 1 cut(s) 536
AccII CGCG 1 cut(s) 626
AciI CCGC 3 cut(s) 259, 357, 380
AclWI GGATC 3 cut(s) 92, 330, 657
AcyI GRCGYC 2 cut(s) 125, 477
AgsI TTSAA 1 cut(s) 269
AjiI CACGTC 1 cut(s) 342
AluBI AGCT 2 cut(s) 319, 406
AluI AGCT 2 cut(s) 319, 406
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 3 cut(s) 92, 330, 657
Ama87I CYCGRG 2 cut(s) 437, 451
AoxI GGCC 2 cut(s) 539, 562
ApeKI GCWGC 5 cut(s) 316, 514, 517, 578, 586
AspLEI GCGC 3 cut(s) 537, 626, 628
AspS9I GGNCC 1 cut(s) 562
AsuHPI GGTGA 2 cut(s) 304, 361
AsuII TTCGAA 1 cut(s) 368
AvaI CYCGRG 2 cut(s) 437, 451
BaeGI GKGCMC 1 cut(s) 145
BanII GRGCYC 1 cut(s) 659
BbvCI CCTCAGC 1 cut(s) 395
BbvI GCAGC 5 cut(s) 328, 501, 504, 590, 598
BccI CCATC 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 2 cut(s) 360, 635
BfmI CTRYAG 1 cut(s) 498
BisI GCNGC 6 cut(s) 317, 357, 515, 518, 579, 587
BlpI GCTNAGC 1 cut(s) 407
BlsI GCNGC 6 cut(s) 318, 358, 516, 519, 580, 588
BmeT110I CYCGRG 2 cut(s) 437, 451
BmgBI CACGTC 1 cut(s) 342
BmgT120I GGNCC 1 cut(s) 562
BmiI GGNNCC 2 cut(s) 458, 658
BmsI GCATC 2 cut(s) 493, 522
BpmI CTGGAG 1 cut(s) 443
Bpu10I CCTNAGC 1 cut(s) 395
Bpu1102I GCTNAGC 1 cut(s) 407
Bpu14I TTCGAA 1 cut(s) 368
BpuEI CTTGAG 1 cut(s) 395
BsaHI GRCGYC 2 cut(s) 125, 477
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bse118I RCCGGY 1 cut(s) 668
Bse1I ACTGG 2 cut(s) 542, 565
BseGI GGATG 2 cut(s) 537, 624
BseMII CTCAG 2 cut(s) 59, 386
BseNI ACTGG 2 cut(s) 542, 565
BsePI GCGCGC 1 cut(s) 624
BseRI GAGGAG 5 cut(s) 211, 214, 217, 541, 649
BseSI GKGCMC 1 cut(s) 145
BseXI GCAGC 5 cut(s) 328, 501, 504, 590, 598
BseYI CCCAGC 1 cut(s) 604
BsgI GTGCAG 1 cut(s) 571
Bsh1236I CGCG 1 cut(s) 626
Bsh1285I CGRYCG 1 cut(s) 211
BshFI GGCC 2 cut(s) 541, 564
BsiEI CGRYCG 1 cut(s) 211
BsiHKCI CYCGRG 2 cut(s) 437, 451
BsiSI CCGG 1 cut(s) 669
BsmAI GTCTC 1 cut(s) 152
BsmBI CGTCTC 1 cut(s) 152
BsnI GGCC 2 cut(s) 541, 564
BsoBI CYCGRG 2 cut(s) 437, 451
Bsp119I TTCGAA 1 cut(s) 368
Bsp1286I GDGCHC 2 cut(s) 145, 659
Bsp143I GATC 8 cut(s) 3, 88, 97, 208, 282, 322, 336, 649
Bsp1720I GCTNAGC 1 cut(s) 407
BspACI CCGC 3 cut(s) 259, 357, 380
BspANI GGCC 2 cut(s) 541, 564
BspCNI CTCAG 2 cut(s) 58, 387
BspFNI CGCG 1 cut(s) 626
BspLI GGNNCC 2 cut(s) 458, 658
BspPI GGATC 3 cut(s) 92, 330, 657
BspT104I TTCGAA 1 cut(s) 368
BsrFI RCCGGY 1 cut(s) 668
BsrI ACTGG 2 cut(s) 542, 565
BssAI RCCGGY 1 cut(s) 668
BssHII GCGCGC 1 cut(s) 624
BssMI GATC 8 cut(s) 3, 88, 97, 208, 282, 322, 336, 649
BssNI GRCGYC 2 cut(s) 125, 477
Bst4CI ACNGT 1 cut(s) 191
BstACI GRCGYC 2 cut(s) 125, 477
BstAPI GCANNNNNTGC 1 cut(s) 595
BstBI TTCGAA 1 cut(s) 368
BstC8I GCNNGC 3 cut(s) 539, 626, 670
BstDEI CTNAG 3 cut(s) 45, 395, 407
BstF5I GGATG 2 cut(s) 537, 624
BstFNI CGCG 1 cut(s) 626
BstHHI GCGC 3 cut(s) 537, 626, 628
BstKTI GATC 8 cut(s) 6, 91, 100, 211, 285, 325, 339, 652
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 8 cut(s) 3, 88, 97, 208, 282, 322, 336, 649
BstMCI CGRYCG 1 cut(s) 211
BstMWI GCNNNNNNNGC 3 cut(s) 242, 503, 595
BstNSI RCATGY 1 cut(s) 467
BstSFI CTRYAG 1 cut(s) 498
BstSLI GKGCMC 1 cut(s) 145
BstUI CGCG 1 cut(s) 626
BstV1I GCAGC 5 cut(s) 328, 501, 504, 590, 598
BstX2I RGATCY 2 cut(s) 322, 649
BstYI RGATCY 2 cut(s) 322, 649
BsuRI GGCC 2 cut(s) 541, 564
BtrI CACGTC 1 cut(s) 342
BtsCI GGATG 2 cut(s) 537, 624
Cac8I GCNNGC 3 cut(s) 539, 626, 670
CfoI GCGC 3 cut(s) 537, 626, 628
Cfr10I RCCGGY 1 cut(s) 668
Cfr13I GGNCC 1 cut(s) 562
CseI GACGC 3 cut(s) 133, 225, 485
CviAII CATG 3 cut(s) 286, 464, 473
DdeI CTNAG 3 cut(s) 45, 395, 407
DpnI GATC 8 cut(s) 5, 90, 99, 210, 284, 324, 338, 651
DpnII GATC 8 cut(s) 3, 88, 97, 208, 282, 322, 336, 649
EciI GGCGGA 1 cut(s) 395
Eco24I GRGCYC 1 cut(s) 659
Eco88I CYCGRG 2 cut(s) 437, 451
EcoT38I GRGCYC 1 cut(s) 659
Esp3I CGTCTC 1 cut(s) 152
FaeI CATG 3 cut(s) 289, 467, 476
FaiI YATR 7 cut(s) 9, 217, 287, 312, 465, 474, 570
FatI CATG 3 cut(s) 285, 463, 472
FauI CCCGC 1 cut(s) 252
Fnu4HI GCNGC 6 cut(s) 317, 357, 515, 518, 579, 587
FokI GGATG 2 cut(s) 544, 631
FriOI GRGCYC 1 cut(s) 659
Fsp4HI GCNGC 6 cut(s) 317, 357, 515, 518, 579, 587
FspBI CTAG 2 cut(s) 360, 635
FspI TGCGCA 1 cut(s) 536
GlaI GCGC 3 cut(s) 536, 625, 627
GluI GCNGC 6 cut(s) 317, 357, 515, 518, 579, 587
GsaI CCCAGC 1 cut(s) 608
GsuI CTGGAG 1 cut(s) 443
HaeIII GGCC 2 cut(s) 541, 564
HapII CCGG 1 cut(s) 669
HgaI GACGC 3 cut(s) 133, 225, 485
HhaI GCGC 3 cut(s) 537, 626, 628
Hin1I GRCGYC 2 cut(s) 125, 477
Hin1II CATG 3 cut(s) 289, 467, 476
Hin6I GCGC 3 cut(s) 535, 624, 626
HinP1I GCGC 3 cut(s) 535, 624, 626
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HinfI GANTC 2 cut(s) 295, 370
HpaI GTTAAC 1 cut(s) 469
HpaII CCGG 1 cut(s) 669
HphI GGTGA 2 cut(s) 304, 361
Hpy166II GTNNAC 1 cut(s) 469
Hpy188I TCNGA 3 cut(s) 123, 300, 695
Hpy188III TCNNGA 2 cut(s) 374, 388
Hpy8I GTNNAC 1 cut(s) 469
Hpy99I CGWCG 6 cut(s) 127, 241, 244, 346, 485, 668
HpyAV CCTTC 3 cut(s) 36, 114, 241
HpyCH4III ACNGT 1 cut(s) 191
HpyCH4IV ACGT 2 cut(s) 341, 483
HpyCH4V TGCA 3 cut(s) 520, 552, 598
HpyF10VI GCNNNNNNNGC 3 cut(s) 242, 503, 595
HpyF3I CTNAG 3 cut(s) 45, 395, 407
HpySE526I ACGT 2 cut(s) 341, 483
Hsp92I GRCGYC 2 cut(s) 125, 477
Hsp92II CATG 3 cut(s) 289, 467, 476
HspAI GCGC 3 cut(s) 535, 624, 626
KroI GCCGGC 1 cut(s) 668
KroNI GCCGGC 1 cut(s) 670
KspAI GTTAAC 1 cut(s) 469
Kzo9I GATC 8 cut(s) 3, 88, 97, 208, 282, 322, 336, 649
LmnI GCTCC 3 cut(s) 204, 462, 662
Lsp1109I GCAGC 5 cut(s) 328, 501, 504, 590, 598
LweI GCATC 2 cut(s) 493, 522
MaeI CTAG 2 cut(s) 360, 635
MaeII ACGT 2 cut(s) 341, 483
MaeIII GTNAC 1 cut(s) 484
MalI GATC 8 cut(s) 5, 90, 99, 210, 284, 324, 338, 651
MboI GATC 8 cut(s) 3, 88, 97, 208, 282, 322, 336, 649
MboII GAAGA 3 cut(s) 53, 56, 346
MfeI CAATTG 1 cut(s) 269
MflI RGATCY 2 cut(s) 322, 649
MhlI GDGCHC 2 cut(s) 145, 659
MluCI AATT 3 cut(s) 269, 492, 681
MmeI TCCRAC 1 cut(s) 146
MreI CGCCGGCG 1 cut(s) 668
MroNI GCCGGC 1 cut(s) 668
MseI TTAA 1 cut(s) 468
MslI CAYNNNNRTG 2 cut(s) 290, 344
MspA1I CMGCKG 1 cut(s) 319
MspI CCGG 1 cut(s) 669
MunI CAATTG 1 cut(s) 269
MvnI CGCG 1 cut(s) 626
MwoI GCNNNNNNNGC 3 cut(s) 242, 503, 595
NaeI GCCGGC 1 cut(s) 670
NdeII GATC 8 cut(s) 3, 88, 97, 208, 282, 322, 336, 649
NgoMIV GCCGGC 1 cut(s) 668
NlaIII CATG 3 cut(s) 289, 467, 476
NlaIV GGNNCC 2 cut(s) 458, 658
NsbI TGCGCA 1 cut(s) 536
NspI RCATGY 1 cut(s) 467
NspV TTCGAA 1 cut(s) 368
PaeR7I CTCGAG 2 cut(s) 437, 451
PauI GCGCGC 1 cut(s) 624
PdiI GCCGGC 1 cut(s) 670
PfeI GAWTC 2 cut(s) 295, 370
PkrI GCNGC 6 cut(s) 318, 358, 516, 519, 580, 588
Ple19I CGATCG 1 cut(s) 211
PspFI CCCAGC 1 cut(s) 604
PspN4I GGNNCC 2 cut(s) 458, 658
PspPI GGNCC 1 cut(s) 562
PspXI VCTCGAGB 2 cut(s) 437, 451
PsuI RGATCY 2 cut(s) 322, 649
PteI GCGCGC 1 cut(s) 624
PvuI CGATCG 1 cut(s) 211
PvuII CAGCTG 1 cut(s) 319
RseI CAYNNNNRTG 2 cut(s) 290, 344
SaqAI TTAA 1 cut(s) 468
SatI GCNGC 6 cut(s) 317, 357, 515, 518, 579, 587
Sau3AI GATC 8 cut(s) 3, 88, 97, 208, 282, 322, 336, 649
Sau96I GGNCC 1 cut(s) 562
SduI GDGCHC 2 cut(s) 145, 659
SetI ASST 9 cut(s) 76, 106, 121, 321, 344, 387, 408, 438, 486
SfaNI GCATC 2 cut(s) 493, 522
SfcI CTRYAG 1 cut(s) 498
Sfr274I CTCGAG 2 cut(s) 437, 451
SfuI TTCGAA 1 cut(s) 368
SgrAI CRCCGGYG 1 cut(s) 668
SlaI CTCGAG 2 cut(s) 437, 451
SmiMI CAYNNNNRTG 2 cut(s) 290, 344
SmlI CTYRAG 3 cut(s) 374, 437, 451
SmoI CTYRAG 3 cut(s) 374, 437, 451
Sse9I AATT 3 cut(s) 269, 492, 681
SsiI CCGC 3 cut(s) 259, 357, 380
SspMI CTAG 2 cut(s) 360, 635
TaaI ACNGT 1 cut(s) 191
TaiI ACGT 2 cut(s) 344, 486
TaqI TCGA 4 cut(s) 344, 368, 438, 452
TasI AATT 3 cut(s) 269, 492, 681
TauI GCSGC 1 cut(s) 359
TfiI GAWTC 2 cut(s) 295, 370
Tru1I TTAA 1 cut(s) 468
Tru9I TTAA 1 cut(s) 468
TseI GCWGC 5 cut(s) 316, 514, 517, 578, 586
TspDTI ATGAA 1 cut(s) 125
TspGWI ACGGA 1 cut(s) 652
XceI RCATGY 1 cut(s) 467
XhoI CTCGAG 2 cut(s) 437, 451
XspI CTAG 2 cut(s) 360, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.