Rh2AG133400

At4g15545-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0019896
Physical Location & Seq
Reverse (-)
12039030 .. 12039299
270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG133400.1

Sequence Viewer

Length: 270 bp
ATGGTGGTGATTCCTAGCGACCCGTACGAGCAGCTGGATCTGGCGTGGAAAATCACGTCGATGGCGACAGCCGCTAGGGTTTCGAATCTCGAGGCGGAGGTCGGGAGGCTGAGGCAGAAGATGAGCGAGAAAGATAGGGTTATTTTTGACCTCGAGGAAAGAGCCTCGCGGCTCCATCATGTTAACCACGACACCGGCGTAACCCGAATTGCTGTAGACGCGGGGTGGGCTGCTGCAGGGGAGGAGGATGCGCAGGCCTGTTGTGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

89

Amino Acids

9.82

Weight (kDa)

5.04

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019896)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0099961
rosa_laevigata RLG00000011632
rosa_multiflora Rmu_co8036340.1_g000001 Rmu_sc0006675.1_g000006 Rmu_sc0007877.1_g000022
rosa_samantha Rh2AG133400 Rh6AG194200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 152
Acc16I TGCGCA 1 cut(s) 252
AccI GTMKAC 1 cut(s) 216
AccII CGCG 2 cut(s) 169, 221
AciI CCGC 4 cut(s) 72, 95, 169, 221
AclWI GGATC 1 cut(s) 45
AfaI GTAC 1 cut(s) 26
AjiI CACGTC 1 cut(s) 57
AluBI AGCT 1 cut(s) 34
AluI AGCT 1 cut(s) 34
AlwI GGATC 1 cut(s) 45
Ama87I CYCGRG 2 cut(s) 89, 152
AoxI GGCC 1 cut(s) 255
ApeKI GCWGC 3 cut(s) 31, 230, 233
AspLEI GCGC 1 cut(s) 253
AsuHPI GGTGA 1 cut(s) 19
AsuII TTCGAA 1 cut(s) 83
AvaI CYCGRG 2 cut(s) 89, 152
BbvCI CCTCAGC 1 cut(s) 110
BbvI GCAGC 3 cut(s) 43, 217, 220
BccI CCATC 2 cut(s) 55, 183
BfaI CTAG 2 cut(s) 15, 75
BfmI CTRYAG 2 cut(s) 213, 234
BisI GCNGC 5 cut(s) 32, 72, 170, 231, 234
BlsI GCNGC 5 cut(s) 33, 73, 171, 232, 235
BmeT110I CYCGRG 2 cut(s) 89, 152
BmgBI CACGTC 1 cut(s) 57
BmiI GGNNCC 1 cut(s) 173
BmsI GCATC 1 cut(s) 238
Bpu10I CCTNAGC 1 cut(s) 110
Bpu14I TTCGAA 1 cut(s) 83
Bse118I RCCGGY 1 cut(s) 194
BseGI GGATG 1 cut(s) 253
BseMII CTCAG 1 cut(s) 101
BseRI GAGGAG 1 cut(s) 257
BseXI GCAGC 3 cut(s) 43, 217, 220
Bsh1236I CGCG 2 cut(s) 169, 221
BshFI GGCC 1 cut(s) 257
BsiHKCI CYCGRG 2 cut(s) 89, 152
BsiSI CCGG 1 cut(s) 195
BsiWI CGTACG 1 cut(s) 24
BsnI GGCC 1 cut(s) 257
BsoBI CYCGRG 2 cut(s) 89, 152
Bsp119I TTCGAA 1 cut(s) 83
Bsp143I GATC 1 cut(s) 37
BspACI CCGC 4 cut(s) 72, 95, 169, 221
BspANI GGCC 1 cut(s) 257
BspCNI CTCAG 1 cut(s) 102
BspFNI CGCG 2 cut(s) 169, 221
BspLI GGNNCC 1 cut(s) 173
BspMAI CTGCAG 1 cut(s) 238
BspPI GGATC 1 cut(s) 45
BspT104I TTCGAA 1 cut(s) 83
BsrFI RCCGGY 1 cut(s) 194
BssAI RCCGGY 1 cut(s) 194
BssMI GATC 1 cut(s) 37
BstBI TTCGAA 1 cut(s) 83
BstC8I GCNNGC 1 cut(s) 255
BstDEI CTNAG 1 cut(s) 110
BstF5I GGATG 1 cut(s) 253
BstFNI CGCG 2 cut(s) 169, 221
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 1 cut(s) 40
BstMBI GATC 1 cut(s) 37
BstMWI GCNNNNNNNGC 4 cut(s) 71, 218, 227, 263
BstSFI CTRYAG 2 cut(s) 213, 234
BstUI CGCG 2 cut(s) 169, 221
BstV1I GCAGC 3 cut(s) 43, 217, 220
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 1 cut(s) 257
BtrI CACGTC 1 cut(s) 57
BtsCI GGATG 1 cut(s) 253
Cac8I GCNNGC 1 cut(s) 255
CfoI GCGC 1 cut(s) 253
Cfr10I RCCGGY 1 cut(s) 194
CseI GACGC 1 cut(s) 227
Csp6I GTAC 1 cut(s) 25
CviAII CATG 1 cut(s) 179
CviJI RGCY 7 cut(s) 34, 71, 109, 164, 172, 230, 257
CviKI_1 RGCY 7 cut(s) 34, 71, 109, 164, 172, 230, 257
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 1 cut(s) 110
DpnI GATC 1 cut(s) 39
DpnII GATC 1 cut(s) 37
EciI GGCGGA 1 cut(s) 110
Eco147I AGGCCT 1 cut(s) 257
Eco88I CYCGRG 2 cut(s) 89, 152
FaeI CATG 1 cut(s) 182
FaiI YATR 1 cut(s) 180
FatI CATG 1 cut(s) 178
FauI CCCGC 1 cut(s) 214
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 5 cut(s) 32, 72, 170, 231, 234
FokI GGATG 1 cut(s) 260
Fsp4HI GCNGC 5 cut(s) 32, 72, 170, 231, 234
FspBI CTAG 2 cut(s) 15, 75
FspI TGCGCA 1 cut(s) 252
GlaI GCGC 1 cut(s) 252
GluI GCNGC 5 cut(s) 32, 72, 170, 231, 234
HaeIII GGCC 1 cut(s) 257
HapII CCGG 1 cut(s) 195
HgaI GACGC 1 cut(s) 227
HhaI GCGC 1 cut(s) 253
Hin1II CATG 1 cut(s) 182
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 2 cut(s) 10, 85
HpaI GTTAAC 1 cut(s) 184
HpaII CCGG 1 cut(s) 195
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 2 cut(s) 184, 217
Hpy188III TCNNGA 2 cut(s) 89, 103
Hpy8I GTNNAC 2 cut(s) 184, 217
Hpy99I CGWCG 1 cut(s) 61
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 1 cut(s) 236
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 218, 227, 263
HpyF3I CTNAG 1 cut(s) 110
HpySE526I ACGT 1 cut(s) 56
Hsp92II CATG 1 cut(s) 182
HspAI GCGC 1 cut(s) 251
KspAI GTTAAC 1 cut(s) 184
Kzo9I GATC 1 cut(s) 37
LmnI GCTCC 1 cut(s) 177
LpnPI CCDG 5 cut(s) 20, 26, 208, 222, 239
Lsp1109I GCAGC 3 cut(s) 43, 217, 220
LweI GCATC 1 cut(s) 238
MaeI CTAG 2 cut(s) 15, 75
MaeII ACGT 1 cut(s) 56
MaeIII GTNAC 1 cut(s) 199
MalI GATC 1 cut(s) 39
MboI GATC 1 cut(s) 37
MboII GAAGA 1 cut(s) 130
MflI RGATCY 1 cut(s) 37
MluCI AATT 1 cut(s) 207
MnlI CCTC 9 cut(s) 85, 91, 99, 105, 148, 161, 175, 235, 238
MseI TTAA 1 cut(s) 183
MslI CAYNNNNRTG 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 34
MspI CCGG 1 cut(s) 195
MvnI CGCG 2 cut(s) 169, 221
MwoI GCNNNNNNNGC 4 cut(s) 71, 218, 227, 263
NdeII GATC 1 cut(s) 37
NlaIII CATG 1 cut(s) 182
NlaIV GGNNCC 1 cut(s) 173
NsbI TGCGCA 1 cut(s) 252
NspV TTCGAA 1 cut(s) 83
PaeR7I CTCGAG 2 cut(s) 89, 152
PceI AGGCCT 1 cut(s) 257
PcsI WCGNNNNNNNCGW 2 cut(s) 62, 195
PfeI GAWTC 2 cut(s) 10, 85
Pfl23II CGTACG 1 cut(s) 24
PkrI GCNGC 5 cut(s) 33, 73, 171, 232, 235
PspLI CGTACG 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 173
PspXI VCTCGAGB 1 cut(s) 152
PstI CTGCAG 1 cut(s) 238
PsuI RGATCY 1 cut(s) 37
PvuII CAGCTG 1 cut(s) 34
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
RseI CAYNNNNRTG 1 cut(s) 59
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 5 cut(s) 32, 72, 170, 231, 234
Sau3AI GATC 1 cut(s) 37
SetI ASST 4 cut(s) 36, 59, 102, 153
SfaNI GCATC 1 cut(s) 238
SfcI CTRYAG 2 cut(s) 213, 234
Sfr274I CTCGAG 2 cut(s) 89, 152
SfuI TTCGAA 1 cut(s) 83
SgrAI CRCCGGYG 1 cut(s) 194
SlaI CTCGAG 2 cut(s) 89, 152
SmiMI CAYNNNNRTG 1 cut(s) 59
SmlI CTYRAG 2 cut(s) 89, 152
SmoI CTYRAG 2 cut(s) 89, 152
Sse9I AATT 1 cut(s) 207
SseBI AGGCCT 1 cut(s) 257
SsiI CCGC 4 cut(s) 72, 95, 169, 221
SspMI CTAG 2 cut(s) 15, 75
StuI AGGCCT 1 cut(s) 257
TaiI ACGT 1 cut(s) 59
TaqI TCGA 4 cut(s) 59, 83, 90, 153
TasI AATT 1 cut(s) 207
TauI GCSGC 2 cut(s) 74, 172
TfiI GAWTC 2 cut(s) 10, 85
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TseI GCWGC 3 cut(s) 31, 230, 233
XhoI CTCGAG 2 cut(s) 89, 152
XmiI GTMKAC 1 cut(s) 216
XspI CTAG 2 cut(s) 15, 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.