Rh6AG194200

At4g15545-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0019896
Physical Location & Seq
Reverse (-)
34863756 .. 34864058
303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG194200.1

Sequence Viewer

Length: 303 bp
ATGTCGCAAGGAAGCGGAAGCGGCGTCGGCGGAGGAGGGGGGGATTTTCAATTGCCGAACGAGATCATGGTGGTGATTCCGAGCGACCCGTACGAGCAGCTGGATCTGGCGAGGAAGATCACGTCGATGGCGATAACCGCTAGGGTTTCGAATCTCGAGGCTGAGGTCGGGAGGCTAAGGCAGAAGTTGAGCGAGAAAGATAGGGTTATTTTTGACCTCAAGGAAAGAGCCTTGCGGCTCCAGCATGTTAACCACGATGCCGGCGTAACTCAAATTGCTGTAGACGCGGGTGGGCAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

100

Amino Acids

10.73

Weight (kDa)

5.59

Isoelectric Point (pI)

33.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019896)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0099961
rosa_laevigata RLG00000011632
rosa_multiflora Rmu_co8036340.1_g000001 Rmu_sc0006675.1_g000006 Rmu_sc0007877.1_g000022
rosa_samantha Rh2AG133400 Rh6AG194200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 282
AccII CGCG 1 cut(s) 287
AciI CCGC 6 cut(s) 15, 21, 30, 138, 235, 287
AclWI GGATC 1 cut(s) 111
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 1 cut(s) 92
AgsI TTSAA 1 cut(s) 50
AjiI CACGTC 1 cut(s) 123
AluBI AGCT 2 cut(s) 100, 298
AluI AGCT 2 cut(s) 100, 298
AlwI GGATC 1 cut(s) 111
Ama87I CYCGRG 1 cut(s) 155
ApeKI GCWGC 2 cut(s) 97, 295
AsuHPI GGTGA 1 cut(s) 85
AsuII TTCGAA 1 cut(s) 149
AvaI CYCGRG 1 cut(s) 155
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 109
BccI CCATC 1 cut(s) 121
BfaI CTAG 1 cut(s) 141
BfmI CTRYAG 2 cut(s) 279, 299
BisI GCNGC 4 cut(s) 22, 98, 236, 296
BlsI GCNGC 4 cut(s) 23, 99, 237, 297
BmeT110I CYCGRG 1 cut(s) 155
BmgBI CACGTC 1 cut(s) 123
BmiI GGNNCC 1 cut(s) 239
BmsI GCATC 1 cut(s) 247
BpmI CTGGAG 1 cut(s) 224
Bpu10I CCTNAGC 2 cut(s) 162, 176
Bpu14I TTCGAA 1 cut(s) 149
BpuEI CTTGAG 1 cut(s) 203
BsaHI GRCGYC 1 cut(s) 24
Bse118I RCCGGY 1 cut(s) 260
BseMII CTCAG 1 cut(s) 153
BseRI GAGGAG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 109
Bsh1236I CGCG 1 cut(s) 287
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 1 cut(s) 261
BsiWI CGTACG 1 cut(s) 90
BsoBI CYCGRG 1 cut(s) 155
Bsp119I TTCGAA 1 cut(s) 149
Bsp143I GATC 3 cut(s) 63, 103, 117
BspACI CCGC 6 cut(s) 15, 21, 30, 138, 235, 287
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 1 cut(s) 287
BspLI GGNNCC 1 cut(s) 239
BspPI GGATC 1 cut(s) 111
BspT104I TTCGAA 1 cut(s) 149
BsrFI RCCGGY 1 cut(s) 260
BssAI RCCGGY 1 cut(s) 260
BssMI GATC 3 cut(s) 63, 103, 117
BssNI GRCGYC 1 cut(s) 24
BstACI GRCGYC 1 cut(s) 24
BstBI TTCGAA 1 cut(s) 149
BstC8I GCNNGC 1 cut(s) 262
BstDEI CTNAG 2 cut(s) 162, 176
BstFNI CGCG 1 cut(s) 287
BstKTI GATC 3 cut(s) 66, 106, 120
BstMBI GATC 3 cut(s) 63, 103, 117
BstMWI GCNNNNNNNGC 5 cut(s) 21, 27, 137, 241, 284
BstNSI RCATGY 1 cut(s) 248
BstSFI CTRYAG 2 cut(s) 279, 299
BstUI CGCG 1 cut(s) 287
BstV1I GCAGC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 103
BstYI RGATCY 1 cut(s) 103
BtrI CACGTC 1 cut(s) 123
Cac8I GCNNGC 1 cut(s) 262
Cfr10I RCCGGY 1 cut(s) 260
CseI GACGC 2 cut(s) 13, 293
Csp6I GTAC 1 cut(s) 91
CviAII CATG 2 cut(s) 67, 245
CviJI RGCY 6 cut(s) 100, 161, 175, 230, 238, 298
CviKI_1 RGCY 6 cut(s) 100, 161, 175, 230, 238, 298
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 2 cut(s) 162, 176
DpnI GATC 3 cut(s) 65, 105, 119
DpnII GATC 3 cut(s) 63, 103, 117
EciI GGCGGA 1 cut(s) 45
Eco88I CYCGRG 1 cut(s) 155
FaeI CATG 2 cut(s) 70, 248
FaiI YATR 2 cut(s) 68, 246
FatI CATG 2 cut(s) 66, 244
FauI CCCGC 1 cut(s) 280
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 4 cut(s) 22, 98, 236, 296
Fsp4HI GCNGC 4 cut(s) 22, 98, 236, 296
FspBI CTAG 1 cut(s) 141
GluI GCNGC 4 cut(s) 22, 98, 236, 296
GsuI CTGGAG 1 cut(s) 224
HapII CCGG 1 cut(s) 261
HgaI GACGC 2 cut(s) 13, 293
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 2 cut(s) 70, 248
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HinfI GANTC 2 cut(s) 76, 151
HpaI GTTAAC 1 cut(s) 250
HpaII CCGG 1 cut(s) 261
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 2 cut(s) 250, 283
Hpy188I TCNGA 1 cut(s) 81
Hpy188III TCNNGA 2 cut(s) 155, 169
Hpy8I GTNNAC 2 cut(s) 250, 283
Hpy99I CGWCG 2 cut(s) 29, 127
HpyCH4IV ACGT 1 cut(s) 122
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 27, 137, 241, 284
HpyF3I CTNAG 2 cut(s) 162, 176
HpySE526I ACGT 1 cut(s) 122
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 2 cut(s) 70, 248
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
KspAI GTTAAC 1 cut(s) 250
Kzo9I GATC 3 cut(s) 63, 103, 117
LmnI GCTCC 1 cut(s) 243
LpnPI CCDG 4 cut(s) 86, 92, 254, 274
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 1 cut(s) 247
MaeI CTAG 1 cut(s) 141
MaeII ACGT 1 cut(s) 122
MaeIII GTNAC 1 cut(s) 265
MalI GATC 3 cut(s) 65, 105, 119
MboI GATC 3 cut(s) 63, 103, 117
MboII GAAGA 1 cut(s) 127
MfeI CAATTG 1 cut(s) 50
MflI RGATCY 1 cut(s) 103
MluCI AATT 2 cut(s) 50, 273
MnlI CCTC 7 cut(s) 26, 29, 105, 151, 157, 165, 227
MroNI GCCGGC 1 cut(s) 260
MseI TTAA 1 cut(s) 249
MslI CAYNNNNRTG 2 cut(s) 71, 125
MspA1I CMGCKG 2 cut(s) 100, 298
MspI CCGG 1 cut(s) 261
MunI CAATTG 1 cut(s) 50
MvnI CGCG 1 cut(s) 287
MwoI GCNNNNNNNGC 5 cut(s) 21, 27, 137, 241, 284
NaeI GCCGGC 1 cut(s) 262
NdeII GATC 3 cut(s) 63, 103, 117
NgoMIV GCCGGC 1 cut(s) 260
NlaIII CATG 2 cut(s) 70, 248
NlaIV GGNNCC 1 cut(s) 239
NspI RCATGY 1 cut(s) 248
NspV TTCGAA 1 cut(s) 149
PaeR7I CTCGAG 1 cut(s) 155
PcsI WCGNNNNNNNCGW 2 cut(s) 128, 261
PdiI GCCGGC 1 cut(s) 262
PfeI GAWTC 2 cut(s) 76, 151
Pfl23II CGTACG 1 cut(s) 90
PkrI GCNGC 4 cut(s) 23, 99, 237, 297
PspLI CGTACG 1 cut(s) 90
PspN4I GGNNCC 1 cut(s) 239
PsuI RGATCY 1 cut(s) 103
PvuII CAGCTG 2 cut(s) 100, 298
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
RseI CAYNNNNRTG 2 cut(s) 71, 125
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 4 cut(s) 22, 98, 236, 296
Sau3AI GATC 3 cut(s) 63, 103, 117
SetI ASST 5 cut(s) 102, 125, 168, 219, 300
SfaNI GCATC 1 cut(s) 247
SfcI CTRYAG 2 cut(s) 279, 299
Sfr274I CTCGAG 1 cut(s) 155
SfuI TTCGAA 1 cut(s) 149
SlaI CTCGAG 1 cut(s) 155
SmiMI CAYNNNNRTG 2 cut(s) 71, 125
SmlI CTYRAG 2 cut(s) 155, 218
SmoI CTYRAG 2 cut(s) 155, 218
Sse9I AATT 2 cut(s) 50, 273
SsiI CCGC 6 cut(s) 15, 21, 30, 138, 235, 287
SspMI CTAG 1 cut(s) 141
TaiI ACGT 1 cut(s) 125
TaqI TCGA 3 cut(s) 125, 149, 156
TasI AATT 2 cut(s) 50, 273
TauI GCSGC 2 cut(s) 24, 238
TfiI GAWTC 2 cut(s) 76, 151
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TseI GCWGC 2 cut(s) 97, 295
XceI RCATGY 1 cut(s) 248
XhoI CTCGAG 1 cut(s) 155
XmiI GTMKAC 1 cut(s) 282
XspI CTAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.