RLG00000012859

HAUS augmin-like complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0005161
Physical Location & Seq
Reverse (-)
23246887 .. 23254220
7334 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012859

Sequence Viewer

Length: 1851 bp
ATGAGCGGAGCCACACTGTGCGCACTGTTGGGCGATTTGGGGTACGAAGGCGCCGATGCATTGGACCCCGACAGTTTCGAATGGCCTTTTCAATACGACGACGCTCGACCTATTCTCGATTGGGTCTGCTCCAGCCTCCGCCCCTCTAACGTTCTATCCCTCACCGAGCTCTCTCGGTACGAGCAGTTTTTACAGGAGGGAAAGCTATTAGAGGGGGAAGATTTGGACTTTGCTTATGATAGCATTTCTGCCTTTTCTTCCAGCCGAGACAACCAGGAGGCGGTTTTCGCAGCTGAAGAGGGACTCAAAGATATTCGGGATGCAACTCTGGCATATAAATCTGAAGCTTTACAGTTGCAGAAGCAGCTTAGACATCTGGATTCTCAATTTGACATGCTTACTGGTCAAGCTTCTGCTTTGATCCAAGGTAGACGGGCTCGAGTTGCTACAACATCTACTATTAATGGACACCTTACTACCATAGATGACACCCTTTCTGCAAAAAATTTGCAGATGAATGCAGTTCTTGGAAGGATTGCGTCTACAGCACAGGAATTGGCTCATTATCACTCTGGGGATGGGGATGGAATCTATTTAGCATATTCAGACTTTCATCCGTACTTGCTTGGAGATTCATCCTGTATAAAGGAGCTCAATCAGTGGTTTGCCAAGCAGTTGGACACGGGCCCCTTTCGTTTAGTTGCGGAAGATGGAAAGTCCAAGTGTTCATGGGTGAGTCTTGAAGACATCTCAAATATCATTGTGAGAGATTTAGAAAAATCTCAGCATCAACGTGTCTCAGAATTGCAACGACTGCGTTCTATTTTTGGGACAAGTGAAAGACAATGGGTGGAAGCTCAAGTTGAGAATGCTAAGCAACAAGCTATTCTAATGTCACTTAAATCTCAAGTTTCATCAGATGAAGCGCACATTCATCTTGATCTTCATTCCCTTAGGAGGAAACATTCAGAACTTGTCGGAGAACTATCAAATTCTTATAACAAGGAAGAGAAATTGTTATCTGAGACTATTCCGGATCTTTGTTGGGAGCTGGCTCAACTCCAAGACACATACATATTGCAAGGTGATTATGACTTAAAGGTCATGCGACAAGAGTACTATATCAACCGGCAGAAAGCGTTTATAAATCATCTTGTCAATCAACTTGCGAGGCACCAACTGTTAAAGTTAGCTTGCCAGTTGGAAAAGAAACACATGCTTGGAGCATATTCGTTGCTGAAAGTTATTGAATCAGAGGTGCAAGCCTACCTGTCAGCAACTGAAGGCCGTGTGGGTCGTTGCCTAGCCCTAATCCAAGCTGCATCTGATGTACAAGAACAAGGAGGAGTGGATGATCAAGATCACTTTTTGCATGGCGTTAGAGATCTTTTGAGTATCCATTCAAATGCTCAAGCTGGGTTGTCAACATATGTATCAGCTCCGGGTATTGTGCAGCAAATATCTAGTCTTCATTCAGATTTGATGACCCTTGAGTCAGATCTTGAGACCTCTCTTCCAGAGGACAGAAGCAGATGTGTCAACGAGCTGTGCACTCTAATTCAGAGTTTGCAGAAATTGCTGTTTGCATCCTCAACAACTGCACAACCAATACTGACATCTCGGCCATTGATGAAAGAGCTCGATGAAATGGAGAAAATAAATGCAAAACTCTCAGCTGCAGTTGAAGAGGTGACACTAGAGCATCGTAAGAAAAATGAGATAGTTAAACACCACTCCCAAGAAGTTGGGCTTCAACGGGGTGTGTTTGTTGATTTCTTCTGCAATCCTGAGCGTTTGAGGAGTCAAGTTCGGGAGCTAACTGCACGAGTCAGAGCTTTGCAGGTTTCATAA

Protein Analysis

617

Amino Acids

69.24

Weight (kDa)

5.24

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS-augmin3 PF14932 28 - 289 1.4e-82 HAUS augmin-like complex subunit 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 999, 1145
AasI GACNNNNNNGTC 2 cut(s) 1100, 1492
Acc16I TGCGCA 1 cut(s) 22
Acc36I ACCTGC 1 cut(s) 1831
AccB1I GGYRCC 2 cut(s) 50, 1173
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 2 cut(s) 430, 542
AccIII TCCGGA 1 cut(s) 1033
AciI CCGC 4 cut(s) 6, 139, 281, 704
AclI AACGTT 1 cut(s) 150
AclWI GGATC 2 cut(s) 415, 1044
AcoI YGGCCR 1 cut(s) 1622
AcsI RAATTY 2 cut(s) 505, 991
AcuI CTGAAG 3 cut(s) 315, 363, 1302
AcyI GRCGYC 1 cut(s) 51
AdeI CACNNNGTG 1 cut(s) 18
AfaI GTAC 5 cut(s) 44, 179, 620, 1118, 1332
AfiI CCNNNNNNNGG 2 cut(s) 280, 957
AflIII ACRYGT 1 cut(s) 793
AgsI TTSAA 6 cut(s) 92, 743, 1250, 1404, 1685, 1754
AjnI CCWGG 1 cut(s) 273
Alw21I GWGCWC 4 cut(s) 171, 654, 1553, 1641
Alw26I GTCTC 4 cut(s) 261, 802, 1019, 1499
Alw44I GTGCAC 1 cut(s) 1549
AlwI GGATC 2 cut(s) 415, 1044
AlwNI CAGNNNCTG 1 cut(s) 1280
Ama87I CYCGRG 1 cut(s) 438
Aor13HI TCCGGA 1 cut(s) 1033
AoxI GGCC 4 cut(s) 83, 685, 1285, 1622
ApaI GGGCCC 1 cut(s) 689
ApaLI GTGCAC 1 cut(s) 1549
ApeKI GCWGC 5 cut(s) 290, 364, 1319, 1453, 1676
ApoI RAATTY 2 cut(s) 505, 991
ArsI GACNNNNNNTTYG 2 cut(s) 1450, 1482
AseI ATTAAT 1 cut(s) 462
AspLEI GCGC 3 cut(s) 23, 53, 928
AspS9I GGNCC 3 cut(s) 64, 685, 686
AsuC2I CCSGG 1 cut(s) 1443
AsuHPI GGTGA 4 cut(s) 154, 745, 1097, 1702
AsuII TTCGAA 1 cut(s) 78
AvaI CYCGRG 1 cut(s) 438
AvaII GGWCC 1 cut(s) 64
AxyI CCTNAGG 1 cut(s) 953
BaeGI GKGCMC 2 cut(s) 689, 1553
BaeI ACNNNNGTAYC 2 cut(s) 1416, 1449
BanI GGYRCC 2 cut(s) 50, 1173
BanII GRGCYC 5 cut(s) 171, 439, 654, 689, 1641
BauI CACGAG 1 cut(s) 1824
BbsI GAAGAC 2 cut(s) 750, 1460
Bbv12I GWGCWC 4 cut(s) 171, 654, 1553, 1641
BbvI GCAGC 5 cut(s) 302, 376, 1306, 1465, 1663
BccI CCATC 3 cut(s) 572, 578, 704
BceAI ACGGC 1 cut(s) 1272
BciT130I CCWGG 1 cut(s) 275
BciVI GTATCC 1 cut(s) 1406
BclI TGATCA 1 cut(s) 1354
BcnI CCSGG 1 cut(s) 1443
BcoDI GTCTC 4 cut(s) 261, 802, 1019, 1499
BfaI CTAG 3 cut(s) 1304, 1464, 1697
BfmI CTRYAG 2 cut(s) 543, 1677
BfoI RGCGCY 1 cut(s) 54
BfuAI ACCTGC 1 cut(s) 1831
BfuI GTATCC 1 cut(s) 1406
BglII AGATCT 2 cut(s) 1384, 1498
BisI GCNGC 5 cut(s) 291, 365, 1320, 1454, 1677
BlpI GCTNAGC 1 cut(s) 873
BlsI GCNGC 5 cut(s) 292, 366, 1321, 1455, 1678
BmcAI AGTACT 1 cut(s) 1118
Bme1390I CCNGG 2 cut(s) 275, 1443
Bme18I GGWCC 1 cut(s) 64
BmeT110I CYCGRG 1 cut(s) 438
BmgT120I GGNCC 3 cut(s) 64, 685, 686
BmiI GGNNCC 6 cut(s) 10, 52, 66, 687, 688, 1175
BmrFI CCNGG 2 cut(s) 275, 1443
BmsI GCATC 6 cut(s) 46, 310, 796, 1331, 1595, 1711
BpiI GAAGAC 2 cut(s) 750, 1460
BpmI CTGGAG 1 cut(s) 115
Bpu10I CCTNAGC 1 cut(s) 1788
Bpu1102I GCTNAGC 1 cut(s) 873
Bpu14I TTCGAA 1 cut(s) 78
BpuEI CTTGAG 5 cut(s) 843, 891, 1395, 1511, 1523
BpuMI CCSGG 1 cut(s) 1443
BsaBI GATNNNNATC 1 cut(s) 1359
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 1499
BsaJI CCNNGG 1 cut(s) 424
BsaWI WCCGGW 1 cut(s) 1033
Bsc4I CCNNNNNNNGG 2 cut(s) 280, 957
Bse118I RCCGGY 1 cut(s) 1128
Bse1I ACTGG 2 cut(s) 406, 1198
Bse21I CCTNAGG 1 cut(s) 953
Bse8I GATNNNNATC 1 cut(s) 1359
BseAI TCCGGA 1 cut(s) 1033
BseBI CCWGG 1 cut(s) 275
BseDI CCNNGG 1 cut(s) 424
BseGI GGATG 7 cut(s) 325, 583, 589, 613, 635, 1357, 1586
BseJI GATNNNNATC 1 cut(s) 1359
BseLI CCNNNNNNNGG 2 cut(s) 280, 957
BseMII CTCAG 5 cut(s) 797, 813, 1014, 1686, 1779
BseNI ACTGG 2 cut(s) 406, 1198
BseRI GAGGAG 2 cut(s) 1359, 1813
BseSI GKGCMC 2 cut(s) 689, 1553
BseXI GCAGC 5 cut(s) 302, 376, 1306, 1465, 1663
BseYI CCCAGC 1 cut(s) 1415
BsgI GTGCAG 3 cut(s) 1472, 1584, 1806
BshFI GGCC 4 cut(s) 85, 687, 1287, 1624
BshNI GGYRCC 2 cut(s) 50, 1173
BsiHKAI GWGCWC 4 cut(s) 171, 654, 1553, 1641
BsiHKCI CYCGRG 1 cut(s) 438
BsiSI CCGG 3 cut(s) 1034, 1129, 1442
BslFI GGGAC 2 cut(s) 315, 844
BslI CCNNNNNNNGG 2 cut(s) 280, 957
BsmAI GTCTC 4 cut(s) 261, 802, 1019, 1499
BsmFI GGGAC 2 cut(s) 315, 844
BsmI GAATGC 2 cut(s) 523, 874
BsnI GGCC 4 cut(s) 85, 687, 1287, 1624
Bso31I GGTCTC 1 cut(s) 1499
BsoBI CYCGRG 1 cut(s) 438
Bsp119I TTCGAA 1 cut(s) 78
Bsp120I GGGCCC 1 cut(s) 685
Bsp1286I GDGCHC 6 cut(s) 171, 439, 654, 689, 1553, 1641
Bsp13I TCCGGA 1 cut(s) 1033
Bsp1407I TGTACA 1 cut(s) 1330
Bsp143I GATC 7 cut(s) 420, 940, 1036, 1354, 1360, 1384, 1498
Bsp1720I GCTNAGC 1 cut(s) 873
BspACI CCGC 4 cut(s) 6, 139, 281, 704
BspANI GGCC 4 cut(s) 85, 687, 1287, 1624
BspCNI CTCAG 5 cut(s) 796, 812, 1015, 1685, 1780
BspEI TCCGGA 1 cut(s) 1033
BspLI GGNNCC 6 cut(s) 10, 52, 66, 687, 688, 1175
BspMAI CTGCAG 1 cut(s) 1681
BspMI ACCTGC 1 cut(s) 1831
BspPI GGATC 2 cut(s) 415, 1044
BspT104I TTCGAA 1 cut(s) 78
BspT107I GGYRCC 2 cut(s) 50, 1173
BspTNI GGTCTC 1 cut(s) 1499
BsrBI CCGCTC 1 cut(s) 6
BsrFI RCCGGY 1 cut(s) 1128
BsrGI TGTACA 1 cut(s) 1330
BsrI ACTGG 2 cut(s) 406, 1198
BssAI RCCGGY 1 cut(s) 1128
BssECI CCNNGG 1 cut(s) 424
BssMI GATC 7 cut(s) 420, 940, 1036, 1354, 1360, 1384, 1498
BssNI GRCGYC 1 cut(s) 51
BssSI CACGAG 1 cut(s) 1824
BssT1I CCWWGG 1 cut(s) 424
Bst2BI CACGAG 1 cut(s) 1824
Bst2UI CCWGG 1 cut(s) 275
Bst4CI ACNGT 5 cut(s) 18, 27, 74, 354, 1182
Bst6I CTCTTC 4 cut(s) 291, 1002, 1518, 1680
BstACI GRCGYC 1 cut(s) 51
BstAPI GCANNNNNTGC 2 cut(s) 814, 1576
BstAUI TGTACA 1 cut(s) 1330
BstBI TTCGAA 1 cut(s) 78
BstC8I GCNNGC 3 cut(s) 1053, 1195, 1263
BstDEI CTNAG 8 cut(s) 368, 783, 799, 873, 953, 1023, 1672, 1788
BstF5I GGATG 7 cut(s) 325, 583, 589, 613, 635, 1357, 1586
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 3 cut(s) 23, 53, 928
BstKTI GATC 7 cut(s) 423, 943, 1039, 1357, 1363, 1387, 1501
BstMAI GTCTC 4 cut(s) 261, 802, 1019, 1499
BstMBI GATC 7 cut(s) 420, 940, 1036, 1354, 1360, 1384, 1498
BstMWI GCNNNNNNNGC 7 cut(s) 287, 329, 364, 443, 545, 814, 1576
BstNI CCWGG 1 cut(s) 275
BstNSI RCATGY 2 cut(s) 397, 1219
BstSCI CCNGG 2 cut(s) 273, 1441
BstSFI CTRYAG 2 cut(s) 543, 1677
BstSLI GKGCMC 2 cut(s) 689, 1553
BstV1I GCAGC 5 cut(s) 302, 376, 1306, 1465, 1663
BstV2I GAAGAC 2 cut(s) 750, 1460
BstX2I RGATCY 3 cut(s) 1036, 1384, 1498
BstXI CCANNNNNNTGG 2 cut(s) 676, 1745
BstYI RGATCY 3 cut(s) 1036, 1384, 1498
Bsu36I CCTNAGG 1 cut(s) 953
BsuI GTATCC 1 cut(s) 1406
BsuRI GGCC 4 cut(s) 85, 687, 1287, 1624
BtsCI GGATG 7 cut(s) 325, 583, 589, 613, 635, 1357, 1586
BtsIMutI CAGTG 3 cut(s) 14, 23, 665
BveI ACCTGC 1 cut(s) 1831
Cac8I GCNNGC 3 cut(s) 1053, 1195, 1263
CaiI CAGNNNCTG 1 cut(s) 1280
CfoI GCGC 3 cut(s) 23, 53, 928
Cfr10I RCCGGY 1 cut(s) 1128
Cfr13I GGNCC 3 cut(s) 64, 685, 686
CseI GACGC 2 cut(s) 110, 528
Csp6I GTAC 5 cut(s) 43, 178, 619, 1117, 1331
CviAII CATG 5 cut(s) 394, 729, 1105, 1216, 1373
CviQI GTAC 5 cut(s) 43, 178, 619, 1117, 1331
DdeI CTNAG 8 cut(s) 368, 783, 799, 873, 953, 1023, 1672, 1788
DinI GGCGCC 1 cut(s) 52
DpnI GATC 7 cut(s) 422, 942, 1038, 1356, 1362, 1386, 1500
DpnII GATC 7 cut(s) 420, 940, 1036, 1354, 1360, 1384, 1498
DraIII CACNNNGTG 1 cut(s) 18
DrdI GACNNNNNNGTC 2 cut(s) 1100, 1492
DseDI GACNNNNNNGTC 2 cut(s) 1100, 1492
EaeI YGGCCR 1 cut(s) 1622
Eam1104I CTCTTC 4 cut(s) 291, 1002, 1518, 1680
EarI CTCTTC 4 cut(s) 291, 1002, 1518, 1680
EciI GGCGGA 1 cut(s) 128
Ecl136II GAGCTC 3 cut(s) 169, 652, 1639
Eco130I CCWWGG 1 cut(s) 424
Eco24I GRGCYC 5 cut(s) 171, 439, 654, 689, 1641
Eco31I GGTCTC 1 cut(s) 1499
Eco47I GGWCC 1 cut(s) 64
Eco53kI GAGCTC 3 cut(s) 169, 652, 1639
Eco57I CTGAAG 3 cut(s) 315, 363, 1302
Eco81I CCTNAGG 1 cut(s) 953
Eco88I CYCGRG 1 cut(s) 438
EcoICRI GAGCTC 3 cut(s) 169, 652, 1639
EcoO109I RGGNCCY 1 cut(s) 686
EcoRII CCWGG 1 cut(s) 273
EcoT14I CCWWGG 1 cut(s) 424
EcoT22I ATGCAT 1 cut(s) 61
EcoT38I GRGCYC 5 cut(s) 171, 439, 654, 689, 1641
EgeI GGCGCC 1 cut(s) 52
EheI GGCGCC 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 424
FaeI CATG 5 cut(s) 397, 732, 1108, 1219, 1376
FalI AAGNNNNNCTT 2 cut(s) 1734, 1766
FaqI GGGAC 2 cut(s) 315, 844
FatI CATG 5 cut(s) 393, 728, 1104, 1215, 1372
FauNDI CATATG 1 cut(s) 1429
FbaI TGATCA 1 cut(s) 1354
FblI GTMKAC 2 cut(s) 430, 542
Fnu4HI GCNGC 5 cut(s) 291, 365, 1320, 1454, 1677
FokI GGATG 7 cut(s) 332, 590, 596, 600, 622, 1364, 1573
FriOI GRGCYC 5 cut(s) 171, 439, 654, 689, 1641
Fsp4HI GCNGC 5 cut(s) 291, 365, 1320, 1454, 1677
FspAI RTGCGCAY 1 cut(s) 22
FspBI CTAG 3 cut(s) 1304, 1464, 1697
FspI TGCGCA 1 cut(s) 22
GlaI GCGC 3 cut(s) 22, 52, 927
GluI GCNGC 5 cut(s) 291, 365, 1320, 1454, 1677
GsaI CCCAGC 1 cut(s) 1419
GsuI CTGGAG 1 cut(s) 115
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 4 cut(s) 85, 687, 1287, 1624
HapII CCGG 3 cut(s) 1034, 1129, 1442
HgaI GACGC 2 cut(s) 110, 528
HhaI GCGC 3 cut(s) 23, 53, 928
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 5 cut(s) 397, 732, 1108, 1219, 1376
Hin6I GCGC 3 cut(s) 21, 51, 926
HinP1I GCGC 3 cut(s) 21, 51, 926
HincII GTYRAC 2 cut(s) 1425, 1540
HindII GTYRAC 2 cut(s) 1425, 1540
HindIII AAGCTT 2 cut(s) 345, 408
HinfI GANTC 9 cut(s) 303, 380, 588, 632, 736, 1250, 1493, 1801, 1827
HpaII CCGG 3 cut(s) 1034, 1129, 1442
HphI GGTGA 4 cut(s) 154, 745, 1097, 1702
Hpy166II GTNNAC 5 cut(s) 431, 543, 1425, 1540, 1551
Hpy8I GTNNAC 5 cut(s) 431, 543, 1425, 1540, 1551
Hpy99I CGWCG 2 cut(s) 101, 104
HpyAV CCTTC 3 cut(s) 41, 525, 1277
HpyCH4III ACNGT 5 cut(s) 18, 27, 74, 354, 1182
HpyCH4IV ACGT 2 cut(s) 150, 793
HpyF10VI GCNNNNNNNGC 7 cut(s) 287, 329, 364, 443, 545, 814, 1576
HpyF3I CTNAG 8 cut(s) 368, 783, 799, 873, 953, 1023, 1672, 1788
HpySE526I ACGT 2 cut(s) 150, 793
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 5 cut(s) 397, 732, 1108, 1219, 1376
HspAI GCGC 3 cut(s) 21, 51, 926
KasI GGCGCC 1 cut(s) 50
Kpn2I TCCGGA 1 cut(s) 1033
Ksp22I TGATCA 1 cut(s) 1354
Kzo9I GATC 7 cut(s) 420, 940, 1036, 1354, 1360, 1384, 1498
LmnI GCTCC 7 cut(s) 8, 134, 649, 1048, 1223, 1444, 1813
Lsp1109I GCAGC 5 cut(s) 302, 376, 1306, 1465, 1663
LweI GCATC 6 cut(s) 46, 310, 796, 1331, 1595, 1711
MaeI CTAG 3 cut(s) 1304, 1464, 1697
MaeII ACGT 2 cut(s) 150, 793
MaeIII GTNAC 2 cut(s) 894, 1690
MalI GATC 7 cut(s) 422, 942, 1038, 1356, 1362, 1386, 1500
MbiI CCGCTC 1 cut(s) 6
MboI GATC 7 cut(s) 420, 940, 1036, 1354, 1360, 1384, 1498
MflI RGATCY 3 cut(s) 1036, 1384, 1498
MhlI GDGCHC 6 cut(s) 171, 439, 654, 689, 1553, 1641
MluCI AATT 8 cut(s) 386, 505, 554, 803, 991, 1013, 1557, 1574
Mly113I GGCGCC 1 cut(s) 51
MlyI GAGTC 5 cut(s) 297, 745, 1502, 1810, 1836
MmeI TCCRAC 3 cut(s) 657, 958, 1182
Mph1103I ATGCAT 1 cut(s) 61
MroI TCCGGA 1 cut(s) 1033
MseI TTAA 5 cut(s) 462, 900, 1097, 1184, 1725
MslI CAYNNNNRTG 2 cut(s) 792, 1404
MspA1I CMGCKG 2 cut(s) 293, 1676
MspI CCGG 3 cut(s) 1034, 1129, 1442
MspR9I CCNGG 2 cut(s) 275, 1443
Mva1269I GAATGC 2 cut(s) 523, 874
MvaI CCWGG 1 cut(s) 275
MwoI GCNNNNNNNGC 7 cut(s) 287, 329, 364, 443, 545, 814, 1576
NarI GGCGCC 1 cut(s) 51
NciI CCSGG 1 cut(s) 1443
NdeI CATATG 1 cut(s) 1429
NdeII GATC 7 cut(s) 420, 940, 1036, 1354, 1360, 1384, 1498
NlaIII CATG 5 cut(s) 397, 732, 1108, 1219, 1376
NlaIV GGNNCC 6 cut(s) 10, 52, 66, 687, 688, 1175
NmeAIII GCCGAG 2 cut(s) 290, 1600
NmuCI GTSAC 2 cut(s) 894, 1690
NsbI TGCGCA 1 cut(s) 22
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 2 cut(s) 397, 1219
NspV TTCGAA 1 cut(s) 78
PaeR7I CTCGAG 1 cut(s) 438
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 2 cut(s) 523, 874
PfeI GAWTC 4 cut(s) 380, 588, 632, 1250
PkrI GCNGC 5 cut(s) 292, 366, 1321, 1455, 1678
PleI GAGTC 5 cut(s) 297, 744, 1501, 1809, 1835
PluTI GGCGCC 1 cut(s) 54
PpsI GAGTC 5 cut(s) 297, 744, 1501, 1809, 1835
PshBI ATTAAT 1 cut(s) 462
PsiI TTATAA 2 cut(s) 999, 1145
Psp124BI GAGCTC 3 cut(s) 171, 654, 1641
Psp1406I AACGTT 1 cut(s) 150
Psp6I CCWGG 1 cut(s) 273
PspFI CCCAGC 1 cut(s) 1415
PspGI CCWGG 1 cut(s) 273
PspN4I GGNNCC 6 cut(s) 10, 52, 66, 687, 688, 1175
PspOMI GGGCCC 1 cut(s) 685
PspPI GGNCC 3 cut(s) 64, 685, 686
PspXI VCTCGAGB 1 cut(s) 438
PstI CTGCAG 1 cut(s) 1681
PstNI CAGNNNCTG 1 cut(s) 1280
PsuI RGATCY 3 cut(s) 1036, 1384, 1498
PvuII CAGCTG 2 cut(s) 293, 1676
RsaI GTAC 5 cut(s) 44, 179, 620, 1118, 1332
RsaNI GTAC 5 cut(s) 43, 178, 619, 1117, 1331
RseI CAYNNNNRTG 2 cut(s) 792, 1404
SacI GAGCTC 3 cut(s) 171, 654, 1641
SaqAI TTAA 5 cut(s) 462, 900, 1097, 1184, 1725
SatI GCNGC 5 cut(s) 291, 365, 1320, 1454, 1677
Sau3AI GATC 7 cut(s) 420, 940, 1036, 1354, 1360, 1384, 1498
Sau96I GGNCC 3 cut(s) 64, 685, 686
ScaI AGTACT 1 cut(s) 1118
SchI GAGTC 5 cut(s) 297, 745, 1502, 1810, 1836
ScrFI CCNGG 2 cut(s) 275, 1443
SduI GDGCHC 6 cut(s) 171, 439, 654, 689, 1553, 1641
SfaNI GCATC 6 cut(s) 46, 310, 796, 1331, 1595, 1711
SfcI CTRYAG 2 cut(s) 543, 1677
SfoI GGCGCC 1 cut(s) 52
Sfr274I CTCGAG 1 cut(s) 438
SfuI TTCGAA 1 cut(s) 78
SinI GGWCC 1 cut(s) 64
SlaI CTCGAG 1 cut(s) 438
SmiMI CAYNNNNRTG 2 cut(s) 792, 1404
SmlI CTYRAG 6 cut(s) 438, 858, 906, 1410, 1490, 1502
SmoI CTYRAG 6 cut(s) 438, 858, 906, 1410, 1490, 1502
Sse9I AATT 8 cut(s) 386, 505, 554, 803, 991, 1013, 1557, 1574
SsiI CCGC 4 cut(s) 6, 139, 281, 704
SspDI GGCGCC 1 cut(s) 50
SspMI CTAG 3 cut(s) 1304, 1464, 1697
SstI GAGCTC 3 cut(s) 171, 654, 1641
StyD4I CCNGG 2 cut(s) 273, 1441
StyI CCWWGG 1 cut(s) 424
TaaI ACNGT 5 cut(s) 18, 27, 74, 354, 1182
TaiI ACGT 2 cut(s) 153, 796
TaqI TCGA 5 cut(s) 78, 106, 117, 439, 1641
TasI AATT 8 cut(s) 386, 505, 554, 803, 991, 1013, 1557, 1574
TatI WGTACW 2 cut(s) 1116, 1330
TfiI GAWTC 4 cut(s) 380, 588, 632, 1250
Tru1I TTAA 5 cut(s) 462, 900, 1097, 1184, 1725
Tru9I TTAA 5 cut(s) 462, 900, 1097, 1184, 1725
TscAI CASTG 3 cut(s) 21, 30, 665
TseFI GTSAC 2 cut(s) 894, 1690
TseI GCWGC 5 cut(s) 290, 364, 1319, 1453, 1676
Tsp45I GTSAC 2 cut(s) 894, 1690
TspGWI ACGGA 1 cut(s) 606
TspRI CASTG 3 cut(s) 21, 30, 665
VneI GTGCAC 1 cut(s) 1549
VpaK11BI GGWCC 1 cut(s) 64
VspI ATTAAT 1 cut(s) 462
XapI RAATTY 2 cut(s) 505, 991
XceI RCATGY 2 cut(s) 397, 1219
XhoI CTCGAG 1 cut(s) 438
XmiI GTMKAC 2 cut(s) 430, 542
XspI CTAG 3 cut(s) 1304, 1464, 1697
ZrmI AGTACT 1 cut(s) 1118
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.