Rh6DG258400

HAUS augmin-like complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005161
Physical Location & Seq
Forward (+)
44855271 .. 44863205
7935 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG258400.1

Sequence Viewer

Length: 1458 bp
ATGCTTACTGGTCAAGCTTCTGCTTTGATCCAAGGTAGACGGGCTCGAGTTGCTACAACATCTACTATTAATGGACACCTTACTACCATAGATGACACCCTTTCTGCAAAAAATTTGCAGATGAATGCAGTTCTTGGAAGGATTGCGTCTACAGCACAGGAATTGGCTCATTATCACTCTGGGGATGGGGATGGAATCTATTTAGCATATTCAGACTTTCATCCGTACTTGCTTGGAGATTCATCCTGTATAAAGGAGCTCAATCAGTGGTTTGCCAAGCAGTTGGACACGGGCCCCTTTCGTTTAGTTGCTGAAGATGGAAAGTCCAAGTGTTCGTGGGTGAGTCTTGAAGACATCTCCAATATCATTGTGAGAGATTTAGAAAAATCCCAGCATCAACGTGTCTCAGAATTGCAACGACTGCGTTCTATTTTTGGGACAAGTGAAAGACAATGGGTGGAAGCTCAAGTTGAGAATGCTAAGCAACAAGCTATTCTAATGTCACTTAAATCTCAAGTTTCATCAGATGAAGCGCACATTCATCTTGATCTTCATTCCCTTAGGAGGAAACATTCGGAGCTTGTCGGAGAACTATCAAATTCTTATAACAAGGAAGAGAAACTGTTATCTGAGACTATTCCGGATCTGTGTTGGGAGCTGGCTCAACTCCAAGACACATACATATTGCAAGGCGATTATGACTTAAAGGTCATGCGACAAGAGTACTATATCAACCGGCAGAAAGCGTTTATAAATCATCTTGTCAATCAACTGGCGAGGCACCAACTGTTAAAGTTAGCTTGCCAGTTGGAAAAGAAACACATGCTTGGAGCATATTCGTTGCTGAAAGTTATTGAATCAGAGGTGCAAGCCTACCTGTCAGCAACTGAAGGCCGTGTGGGTCGTTGCCTAGCCCTAATCCAAGCTGCATCTGATGTACAAGAACAAGGAGGAGTGGATGATCAAGATCACTTTTTGCATGGCGTTAGAGATCTTTTGAGTATCCATTCAAATGCTCAAGCTGGGTTGTCAACATATGTATCAGCTCCGGGTATTGTGCAGCAAATATCTAGTCTTCATTCAGATTTGATGACCCTTGAGTCGGATCTTGAGACCTCTCTTCCAGAGGACAGAAGCAGATGTGTCAACGAGCTGTGCACTTTAATTCAGAGTTTGCAGAAATTGCTGTTTGCATCTTCAACAACTGCGCAACCAATACTGACATCTCGGCCATTGATGAAAGAGCTTGATGAAATGGAGAAAATAAATGCAAAACTCTCAGCTGCAGTTGAAGAGGTGACACTAGAGCATCGTAAGAAAAATGAGATAGTTAAACACCACTCCCAAGAAGTTGGGCTTCAACGGGGTGTGTTTGTTGATTTCTTCTGCAATTCTGAGCGTTTGAGGAGTCAAGTTCGGGAGCTAACTGCACGAGTCAGAGCTTTGCAGGTTTCATAA

Protein Analysis

485

Amino Acids

54.56

Weight (kDa)

6.03

Isoelectric Point (pI)

42.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS-augmin3 PF14932 2 - 158 1.7e-42 HAUS augmin-like complex subunit 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 606, 752
AasI GACNNNNNNGTC 2 cut(s) 707, 1099
Acc16I TGCGCA 1 cut(s) 1209
Acc36I ACCTGC 1 cut(s) 1438
AccB1I GGYRCC 1 cut(s) 780
AccI GTMKAC 2 cut(s) 37, 149
AccIII TCCGGA 1 cut(s) 640
AclWI GGATC 3 cut(s) 22, 651, 1113
AcoI YGGCCR 1 cut(s) 1229
AcsI RAATTY 2 cut(s) 112, 598
AcuI CTGAAG 2 cut(s) 333, 909
AfaI GTAC 3 cut(s) 227, 725, 939
AfiI CCNNNNNNNGG 2 cut(s) 564, 1102
AflIII ACRYGT 1 cut(s) 400
AgsI TTSAA 6 cut(s) 350, 857, 1011, 1200, 1292, 1361
Alw21I GWGCWC 2 cut(s) 261, 1160
Alw26I GTCTC 3 cut(s) 409, 626, 1106
Alw44I GTGCAC 1 cut(s) 1156
AlwI GGATC 3 cut(s) 22, 651, 1113
AlwNI CAGNNNCTG 1 cut(s) 887
Ama87I CYCGRG 1 cut(s) 45
Aor13HI TCCGGA 1 cut(s) 640
AoxI GGCC 3 cut(s) 292, 892, 1229
ApaI GGGCCC 1 cut(s) 296
ApaLI GTGCAC 1 cut(s) 1156
ApeKI GCWGC 3 cut(s) 926, 1060, 1283
ApoI RAATTY 2 cut(s) 112, 598
ArsI GACNNNNNNTTYG 2 cut(s) 1057, 1089
AseI ATTAAT 1 cut(s) 69
AspLEI GCGC 2 cut(s) 535, 1210
AspS9I GGNCC 2 cut(s) 292, 293
AsuC2I CCSGG 1 cut(s) 1050
AsuHPI GGTGA 2 cut(s) 352, 1309
AvaI CYCGRG 1 cut(s) 45
AxyI CCTNAGG 1 cut(s) 560
BaeGI GKGCMC 2 cut(s) 296, 1160
BaeI ACNNNNGTAYC 2 cut(s) 1023, 1056
BanI GGYRCC 1 cut(s) 780
BanII GRGCYC 3 cut(s) 46, 261, 296
BauI CACGAG 1 cut(s) 1431
BbsI GAAGAC 2 cut(s) 357, 1067
Bbv12I GWGCWC 2 cut(s) 261, 1160
BbvI GCAGC 3 cut(s) 913, 1072, 1270
BccI CCATC 3 cut(s) 179, 185, 311
BceAI ACGGC 1 cut(s) 879
BciVI GTATCC 1 cut(s) 1013
BclI TGATCA 1 cut(s) 961
BcnI CCSGG 1 cut(s) 1050
BcoDI GTCTC 3 cut(s) 409, 626, 1106
BfaI CTAG 3 cut(s) 911, 1071, 1304
BfmI CTRYAG 2 cut(s) 150, 1284
BfuAI ACCTGC 1 cut(s) 1438
BfuI GTATCC 1 cut(s) 1013
BglII AGATCT 1 cut(s) 991
BisI GCNGC 3 cut(s) 927, 1061, 1284
BlpI GCTNAGC 1 cut(s) 480
BlsI GCNGC 3 cut(s) 928, 1062, 1285
BmcAI AGTACT 1 cut(s) 725
Bme1390I CCNGG 1 cut(s) 1050
BmeT110I CYCGRG 1 cut(s) 45
BmgT120I GGNCC 2 cut(s) 292, 293
BmiI GGNNCC 3 cut(s) 294, 295, 782
BmrFI CCNGG 1 cut(s) 1050
BmsI GCATC 4 cut(s) 403, 938, 1202, 1318
BpiI GAAGAC 2 cut(s) 357, 1067
Bpu1102I GCTNAGC 1 cut(s) 480
BpuEI CTTGAG 5 cut(s) 450, 498, 1002, 1118, 1130
BpuMI CCSGG 1 cut(s) 1050
BsaBI GATNNNNATC 1 cut(s) 966
BsaI GGTCTC 1 cut(s) 1106
BsaJI CCNNGG 1 cut(s) 31
BsaWI WCCGGW 1 cut(s) 640
Bsc4I CCNNNNNNNGG 2 cut(s) 564, 1102
Bse118I RCCGGY 1 cut(s) 735
Bse1I ACTGG 3 cut(s) 13, 777, 805
Bse21I CCTNAGG 1 cut(s) 560
Bse8I GATNNNNATC 1 cut(s) 966
BseAI TCCGGA 1 cut(s) 640
BseDI CCNNGG 1 cut(s) 31
BseGI GGATG 5 cut(s) 190, 196, 220, 242, 964
BseJI GATNNNNATC 1 cut(s) 966
BseLI CCNNNNNNNGG 2 cut(s) 564, 1102
BseMII CTCAG 4 cut(s) 420, 621, 1293, 1386
BseNI ACTGG 3 cut(s) 13, 777, 805
BseRI GAGGAG 2 cut(s) 966, 1420
BseSI GKGCMC 2 cut(s) 296, 1160
BseXI GCAGC 3 cut(s) 913, 1072, 1270
BseYI CCCAGC 2 cut(s) 390, 1022
BsgI GTGCAG 2 cut(s) 1079, 1413
BshFI GGCC 3 cut(s) 294, 894, 1231
BshNI GGYRCC 1 cut(s) 780
BsiHKAI GWGCWC 2 cut(s) 261, 1160
BsiHKCI CYCGRG 1 cut(s) 45
BsiSI CCGG 3 cut(s) 641, 736, 1049
BslFI GGGAC 1 cut(s) 451
BslI CCNNNNNNNGG 2 cut(s) 564, 1102
BsmAI GTCTC 3 cut(s) 409, 626, 1106
BsmFI GGGAC 1 cut(s) 451
BsmI GAATGC 2 cut(s) 130, 481
BsnI GGCC 3 cut(s) 294, 894, 1231
Bso31I GGTCTC 1 cut(s) 1106
BsoBI CYCGRG 1 cut(s) 45
Bsp120I GGGCCC 1 cut(s) 292
Bsp1286I GDGCHC 4 cut(s) 46, 261, 296, 1160
Bsp13I TCCGGA 1 cut(s) 640
Bsp1407I TGTACA 1 cut(s) 937
Bsp143I GATC 7 cut(s) 27, 547, 643, 961, 967, 991, 1105
Bsp1720I GCTNAGC 1 cut(s) 480
BspANI GGCC 3 cut(s) 294, 894, 1231
BspCNI CTCAG 4 cut(s) 419, 622, 1292, 1387
BspEI TCCGGA 1 cut(s) 640
BspLI GGNNCC 3 cut(s) 294, 295, 782
BspMAI CTGCAG 1 cut(s) 1288
BspMI ACCTGC 1 cut(s) 1438
BspPI GGATC 3 cut(s) 22, 651, 1113
BspT107I GGYRCC 1 cut(s) 780
BspTNI GGTCTC 1 cut(s) 1106
BsrFI RCCGGY 1 cut(s) 735
BsrGI TGTACA 1 cut(s) 937
BsrI ACTGG 3 cut(s) 13, 777, 805
BssAI RCCGGY 1 cut(s) 735
BssECI CCNNGG 1 cut(s) 31
BssMI GATC 7 cut(s) 27, 547, 643, 961, 967, 991, 1105
BssSI CACGAG 1 cut(s) 1431
BssT1I CCWWGG 1 cut(s) 31
Bst2BI CACGAG 1 cut(s) 1431
Bst4CI ACNGT 2 cut(s) 624, 789
Bst6I CTCTTC 3 cut(s) 609, 1125, 1287
BstAPI GCANNNNNTGC 2 cut(s) 421, 1183
BstAUI TGTACA 1 cut(s) 937
BstC8I GCNNGC 3 cut(s) 660, 802, 870
BstDEI CTNAG 6 cut(s) 406, 480, 560, 630, 1279, 1395
BstF5I GGATG 5 cut(s) 190, 196, 220, 242, 964
BstHHI GCGC 2 cut(s) 535, 1210
BstKTI GATC 7 cut(s) 30, 550, 646, 964, 970, 994, 1108
BstMAI GTCTC 3 cut(s) 409, 626, 1106
BstMBI GATC 7 cut(s) 27, 547, 643, 961, 967, 991, 1105
BstMWI GCNNNNNNNGC 4 cut(s) 50, 152, 421, 1183
BstNSI RCATGY 1 cut(s) 826
BstSCI CCNGG 1 cut(s) 1048
BstSFI CTRYAG 2 cut(s) 150, 1284
BstSLI GKGCMC 2 cut(s) 296, 1160
BstV1I GCAGC 3 cut(s) 913, 1072, 1270
BstV2I GAAGAC 2 cut(s) 357, 1067
BstX2I RGATCY 3 cut(s) 643, 991, 1105
BstXI CCANNNNNNTGG 2 cut(s) 283, 1352
BstYI RGATCY 3 cut(s) 643, 991, 1105
Bsu36I CCTNAGG 1 cut(s) 560
BsuI GTATCC 1 cut(s) 1013
BsuRI GGCC 3 cut(s) 294, 894, 1231
BtsCI GGATG 5 cut(s) 190, 196, 220, 242, 964
BtsIMutI CAGTG 1 cut(s) 272
BveI ACCTGC 1 cut(s) 1438
Cac8I GCNNGC 3 cut(s) 660, 802, 870
CaiI CAGNNNCTG 1 cut(s) 887
CfoI GCGC 2 cut(s) 535, 1210
Cfr10I RCCGGY 1 cut(s) 735
Cfr13I GGNCC 2 cut(s) 292, 293
CseI GACGC 1 cut(s) 135
Csp6I GTAC 3 cut(s) 226, 724, 938
CviAII CATG 3 cut(s) 712, 823, 980
CviQI GTAC 3 cut(s) 226, 724, 938
DdeI CTNAG 6 cut(s) 406, 480, 560, 630, 1279, 1395
DpnI GATC 7 cut(s) 29, 549, 645, 963, 969, 993, 1107
DpnII GATC 7 cut(s) 27, 547, 643, 961, 967, 991, 1105
DrdI GACNNNNNNGTC 2 cut(s) 707, 1099
DseDI GACNNNNNNGTC 2 cut(s) 707, 1099
EaeI YGGCCR 1 cut(s) 1229
Eam1104I CTCTTC 3 cut(s) 609, 1125, 1287
EarI CTCTTC 3 cut(s) 609, 1125, 1287
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 31
Eco24I GRGCYC 3 cut(s) 46, 261, 296
Eco31I GGTCTC 1 cut(s) 1106
Eco53kI GAGCTC 1 cut(s) 259
Eco57I CTGAAG 2 cut(s) 333, 909
Eco81I CCTNAGG 1 cut(s) 560
Eco88I CYCGRG 1 cut(s) 45
EcoICRI GAGCTC 1 cut(s) 259
EcoO109I RGGNCCY 1 cut(s) 293
EcoT14I CCWWGG 1 cut(s) 31
EcoT38I GRGCYC 3 cut(s) 46, 261, 296
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 3 cut(s) 715, 826, 983
FalI AAGNNNNNCTT 2 cut(s) 1341, 1373
FaqI GGGAC 1 cut(s) 451
FatI CATG 3 cut(s) 711, 822, 979
FauNDI CATATG 1 cut(s) 1036
FbaI TGATCA 1 cut(s) 961
FblI GTMKAC 2 cut(s) 37, 149
Fnu4HI GCNGC 3 cut(s) 927, 1061, 1284
FokI GGATG 5 cut(s) 197, 203, 207, 229, 971
FriOI GRGCYC 3 cut(s) 46, 261, 296
Fsp4HI GCNGC 3 cut(s) 927, 1061, 1284
FspBI CTAG 3 cut(s) 911, 1071, 1304
FspI TGCGCA 1 cut(s) 1209
GlaI GCGC 2 cut(s) 534, 1209
GluI GCNGC 3 cut(s) 927, 1061, 1284
GsaI CCCAGC 2 cut(s) 394, 1026
HaeIII GGCC 3 cut(s) 294, 894, 1231
HapII CCGG 3 cut(s) 641, 736, 1049
HgaI GACGC 1 cut(s) 135
HhaI GCGC 2 cut(s) 535, 1210
Hin1II CATG 3 cut(s) 715, 826, 983
Hin6I GCGC 2 cut(s) 533, 1208
HinP1I GCGC 2 cut(s) 533, 1208
HincII GTYRAC 2 cut(s) 1032, 1147
HindII GTYRAC 2 cut(s) 1032, 1147
HindIII AAGCTT 1 cut(s) 15
HinfI GANTC 7 cut(s) 195, 239, 343, 857, 1100, 1408, 1434
HpaII CCGG 3 cut(s) 641, 736, 1049
HphI GGTGA 2 cut(s) 352, 1309
Hpy166II GTNNAC 5 cut(s) 38, 150, 1032, 1147, 1158
Hpy188III TCNNGA 7 cut(s) 347, 545, 641, 965, 1109, 1124, 1418
Hpy8I GTNNAC 5 cut(s) 38, 150, 1032, 1147, 1158
HpyAV CCTTC 2 cut(s) 132, 884
HpyCH4III ACNGT 2 cut(s) 624, 789
HpyCH4IV ACGT 1 cut(s) 400
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 152, 421, 1183
HpyF3I CTNAG 6 cut(s) 406, 480, 560, 630, 1279, 1395
HpySE526I ACGT 1 cut(s) 400
Hsp92II CATG 3 cut(s) 715, 826, 983
HspAI GCGC 2 cut(s) 533, 1208
Kpn2I TCCGGA 1 cut(s) 640
Ksp22I TGATCA 1 cut(s) 961
Kzo9I GATC 7 cut(s) 27, 547, 643, 961, 967, 991, 1105
LmnI GCTCC 6 cut(s) 256, 577, 655, 830, 1051, 1420
Lsp1109I GCAGC 3 cut(s) 913, 1072, 1270
LweI GCATC 4 cut(s) 403, 938, 1202, 1318
MaeI CTAG 3 cut(s) 911, 1071, 1304
MaeII ACGT 1 cut(s) 400
MaeIII GTNAC 2 cut(s) 501, 1297
MalI GATC 7 cut(s) 29, 549, 645, 963, 969, 993, 1107
MboI GATC 7 cut(s) 27, 547, 643, 961, 967, 991, 1105
MboII GAAGA 9 cut(s) 326, 362, 542, 626, 1067, 1112, 1188, 1304, 1375
MflI RGATCY 3 cut(s) 643, 991, 1105
MhlI GDGCHC 4 cut(s) 46, 261, 296, 1160
MluCI AATT 7 cut(s) 112, 161, 410, 598, 1164, 1181, 1390
MlyI GAGTC 4 cut(s) 352, 1109, 1417, 1443
MmeI TCCRAC 4 cut(s) 264, 565, 789, 1083
MnlI CCTC 8 cut(s) 558, 771, 856, 944, 1120, 1126, 1288, 1398
MroI TCCGGA 1 cut(s) 640
MseI TTAA 6 cut(s) 69, 507, 704, 791, 1163, 1332
MslI CAYNNNNRTG 2 cut(s) 399, 1011
MspA1I CMGCKG 1 cut(s) 1283
MspI CCGG 3 cut(s) 641, 736, 1049
MspR9I CCNGG 1 cut(s) 1050
Mva1269I GAATGC 2 cut(s) 130, 481
MwoI GCNNNNNNNGC 4 cut(s) 50, 152, 421, 1183
NciI CCSGG 1 cut(s) 1050
NdeI CATATG 1 cut(s) 1036
NdeII GATC 7 cut(s) 27, 547, 643, 961, 967, 991, 1105
NlaIII CATG 3 cut(s) 715, 826, 983
NlaIV GGNNCC 3 cut(s) 294, 295, 782
NmeAIII GCCGAG 1 cut(s) 1207
NmuCI GTSAC 2 cut(s) 501, 1297
NsbI TGCGCA 1 cut(s) 1209
NspI RCATGY 1 cut(s) 826
PaeR7I CTCGAG 1 cut(s) 45
PctI GAATGC 2 cut(s) 130, 481
PfeI GAWTC 3 cut(s) 195, 239, 857
PkrI GCNGC 3 cut(s) 928, 1062, 1285
PleI GAGTC 4 cut(s) 351, 1108, 1416, 1442
PpsI GAGTC 4 cut(s) 351, 1108, 1416, 1442
PshBI ATTAAT 1 cut(s) 69
PsiI TTATAA 2 cut(s) 606, 752
Psp124BI GAGCTC 1 cut(s) 261
PspFI CCCAGC 2 cut(s) 390, 1022
PspN4I GGNNCC 3 cut(s) 294, 295, 782
PspOMI GGGCCC 1 cut(s) 292
PspPI GGNCC 2 cut(s) 292, 293
PspXI VCTCGAGB 1 cut(s) 45
PstI CTGCAG 1 cut(s) 1288
PstNI CAGNNNCTG 1 cut(s) 887
PsuI RGATCY 3 cut(s) 643, 991, 1105
PvuII CAGCTG 1 cut(s) 1283
RsaI GTAC 3 cut(s) 227, 725, 939
RsaNI GTAC 3 cut(s) 226, 724, 938
RseI CAYNNNNRTG 2 cut(s) 399, 1011
SacI GAGCTC 1 cut(s) 261
SaqAI TTAA 6 cut(s) 69, 507, 704, 791, 1163, 1332
SatI GCNGC 3 cut(s) 927, 1061, 1284
Sau3AI GATC 7 cut(s) 27, 547, 643, 961, 967, 991, 1105
Sau96I GGNCC 2 cut(s) 292, 293
ScaI AGTACT 1 cut(s) 725
SchI GAGTC 4 cut(s) 352, 1109, 1417, 1443
ScrFI CCNGG 1 cut(s) 1050
SduI GDGCHC 4 cut(s) 46, 261, 296, 1160
SfaNI GCATC 4 cut(s) 403, 938, 1202, 1318
SfcI CTRYAG 2 cut(s) 150, 1284
Sfr274I CTCGAG 1 cut(s) 45
SlaI CTCGAG 1 cut(s) 45
SmiMI CAYNNNNRTG 2 cut(s) 399, 1011
SmlI CTYRAG 6 cut(s) 45, 465, 513, 1017, 1097, 1109
SmoI CTYRAG 6 cut(s) 45, 465, 513, 1017, 1097, 1109
Sse9I AATT 7 cut(s) 112, 161, 410, 598, 1164, 1181, 1390
SspMI CTAG 3 cut(s) 911, 1071, 1304
SstI GAGCTC 1 cut(s) 261
StyD4I CCNGG 1 cut(s) 1048
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 2 cut(s) 624, 789
TaiI ACGT 1 cut(s) 403
TaqI TCGA 1 cut(s) 46
TasI AATT 7 cut(s) 112, 161, 410, 598, 1164, 1181, 1390
TatI WGTACW 2 cut(s) 723, 937
TfiI GAWTC 3 cut(s) 195, 239, 857
Tru1I TTAA 6 cut(s) 69, 507, 704, 791, 1163, 1332
Tru9I TTAA 6 cut(s) 69, 507, 704, 791, 1163, 1332
TscAI CASTG 1 cut(s) 272
TseFI GTSAC 2 cut(s) 501, 1297
TseI GCWGC 3 cut(s) 926, 1060, 1283
Tsp45I GTSAC 2 cut(s) 501, 1297
TspGWI ACGGA 1 cut(s) 213
TspRI CASTG 1 cut(s) 272
VneI GTGCAC 1 cut(s) 1156
VspI ATTAAT 1 cut(s) 69
XapI RAATTY 2 cut(s) 112, 598
XceI RCATGY 1 cut(s) 826
XhoI CTCGAG 1 cut(s) 45
XmiI GTMKAC 2 cut(s) 37, 149
XspI CTAG 3 cut(s) 911, 1071, 1304
ZrmI AGTACT 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.