RLG00000013336

receptor-like protein 12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0020640
Physical Location & Seq
Reverse (-)
29993042 .. 29993692
651 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013336

Sequence Viewer

Length: 651 bp
ATGCCGCAGGCACGCTGGAAACTCAAACCAAAACGATCACCTATTATCTATCAAGGCCGGCTATTTTTCAACTGTGATGGTGCTGCTGGCCATGAAACTGACATGGAAGTCTCATTTGTTTCCTTCGATCTCCCTCAGCAAGCCCGTCAGATATTTGAGAGGAACAAGATGGTGAGCCACCCGAGGGTGCTGCTGGCCTGGTTCTCCACATTCGACCACAGTATCAAGAAGCACATTGTCTGCTTTGATCCATTTTCCTGGTTTTTACCCGATTCTCGCCCCACTTGGCTCGAGACACCAATGTCCTCTTCTATTCGTGGATTGCCGAATGAAGAGTTGCAGCGGGAGGTTCGTGACCTTGCGATGGCGCTGGAGCACATCCAACATAAGGGTGTTTCTCATCCCTCGCTCCATCAGCTCTCAAGTTATGTTTGGTGCCATGATGAAATCAAAATCATTAATGTGGATGACTACCAATATGATTTTTGGGATGACCTAGACCCTTCTTCAACAGAGAAGCAGTGGATTGGAAGGAAAGATCATCACTTGAAGCTTTTCAGGAAGGGACTTGAATACCATATGCTCAAAACAGAGGGAGATTGGACTAAGAGGACTTCATTCCTCAAATGTTTCAACATCAATGTCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8

Protein Analysis

217

Amino Acids

25.66

Weight (kDa)

6.86

Isoelectric Point (pI)

53.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020640)

Species Orthologous Gene IDs
rosa_laevigata RLG00000013336
rosa_roxburghii Rroxscaffold_7G00193090
rosa_rugosa Rorug06G0094900
rosa_samantha Rh6AG207200
rosa_wichuraiana Rw6G017970 Rw6G017990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 107, 301
AccB1I GGYRCC 1 cut(s) 435
AciI CCGC 2 cut(s) 5, 343
AclWI GGATC 1 cut(s) 242
AcoI YGGCCR 1 cut(s) 88
AfiI CCNNNNNNNGG 3 cut(s) 184, 364, 388
AgsI TTSAA 5 cut(s) 70, 510, 550, 572, 634
AjnI CCWGG 2 cut(s) 197, 257
AjuI GAANNNNNNNTTGG 2 cut(s) 292, 324
AluBI AGCT 2 cut(s) 418, 553
AluI AGCT 2 cut(s) 418, 553
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 2 cut(s) 115, 287
AlwI GGATC 1 cut(s) 242
Ama87I CYCGRG 2 cut(s) 181, 290
AoxI GGCC 3 cut(s) 55, 88, 195
ApeKI GCWGC 3 cut(s) 83, 190, 340
AseI ATTAAT 1 cut(s) 459
Asp700I GAANNNNTTC 1 cut(s) 554
AspLEI GCGC 1 cut(s) 370
AsuHPI GGTGA 2 cut(s) 30, 184
AvaI CYCGRG 2 cut(s) 181, 290
BaeI ACNNNNGTAYC 2 cut(s) 205, 238
BalI TGGCCA 1 cut(s) 90
BanI GGYRCC 1 cut(s) 435
Bbv12I GWGCWC 1 cut(s) 378
BbvCI CCTCAGC 1 cut(s) 135
BbvI GCAGC 3 cut(s) 70, 177, 352
BccI CCATC 4 cut(s) 71, 163, 358, 420
BcgI CGANNNNNNTGC 2 cut(s) 172, 206
BciT130I CCWGG 2 cut(s) 199, 259
BcoDI GTCTC 2 cut(s) 115, 287
BfaI CTAG 1 cut(s) 497
BfoI RGCGCY 1 cut(s) 371
BisI GCNGC 4 cut(s) 5, 84, 191, 341
BlsI GCNGC 4 cut(s) 6, 85, 192, 342
Bme1390I CCNGG 2 cut(s) 199, 259
BmeT110I CYCGRG 2 cut(s) 181, 290
BmiI GGNNCC 1 cut(s) 437
BmrFI CCNGG 2 cut(s) 199, 259
BpmI CTGGAG 1 cut(s) 392
Bpu10I CCTNAGC 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 406
BsaJI CCNNGG 1 cut(s) 182
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 364, 388
Bse118I RCCGGY 1 cut(s) 57
BseBI CCWGG 2 cut(s) 199, 259
BseDI CCNNGG 1 cut(s) 182
BseGI GGATG 4 cut(s) 378, 400, 472, 496
BseLI CCNNNNNNNGG 3 cut(s) 184, 364, 388
BseMII CTCAG 1 cut(s) 149
BseXI GCAGC 3 cut(s) 70, 177, 352
BshFI GGCC 3 cut(s) 57, 90, 197
BshNI GGYRCC 1 cut(s) 435
BsiHKAI GWGCWC 1 cut(s) 378
BsiHKCI CYCGRG 2 cut(s) 181, 290
BsiSI CCGG 1 cut(s) 58
BslFI GGGAC 1 cut(s) 579
BslI CCNNNNNNNGG 3 cut(s) 184, 364, 388
BsmAI GTCTC 2 cut(s) 115, 287
BsmFI GGGAC 1 cut(s) 579
BsnI GGCC 3 cut(s) 57, 90, 197
BsoBI CYCGRG 2 cut(s) 181, 290
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 4 cut(s) 35, 127, 247, 538
BspACI CCGC 2 cut(s) 5, 343
BspANI GGCC 3 cut(s) 57, 90, 197
BspCNI CTCAG 1 cut(s) 148
BspLI GGNNCC 1 cut(s) 437
BspPI GGATC 1 cut(s) 242
BspT107I GGYRCC 1 cut(s) 435
BsrFI RCCGGY 1 cut(s) 57
BssAI RCCGGY 1 cut(s) 57
BssECI CCNNGG 1 cut(s) 182
BssMI GATC 4 cut(s) 35, 127, 247, 538
Bst2UI CCWGG 2 cut(s) 199, 259
Bst4CI ACNGT 2 cut(s) 74, 221
Bst6I CTCTTC 2 cut(s) 313, 327
BstC8I GCNNGC 6 cut(s) 9, 13, 59, 88, 141, 195
BstDEI CTNAG 2 cut(s) 135, 606
BstF5I GGATG 4 cut(s) 378, 400, 472, 496
BstH2I RGCGCY 1 cut(s) 371
BstHHI GCGC 1 cut(s) 370
BstKTI GATC 4 cut(s) 38, 130, 250, 541
BstMAI GTCTC 2 cut(s) 115, 287
BstMBI GATC 4 cut(s) 35, 127, 247, 538
BstMWI GCNNNNNNNGC 1 cut(s) 415
BstNI CCWGG 2 cut(s) 199, 259
BstSCI CCNGG 2 cut(s) 197, 257
BstV1I GCAGC 3 cut(s) 70, 177, 352
BstXI CCANNNNNNTGG 1 cut(s) 258
BsuRI GGCC 3 cut(s) 57, 90, 197
BtgZI GCGATG 1 cut(s) 377
BtsCI GGATG 4 cut(s) 378, 400, 472, 496
BtsI GCAGTG 1 cut(s) 527
BtsIMutI CAGTG 1 cut(s) 527
Cac8I GCNNGC 6 cut(s) 9, 13, 59, 88, 141, 195
CfoI GCGC 1 cut(s) 370
Cfr10I RCCGGY 1 cut(s) 57
CviAII CATG 3 cut(s) 92, 103, 440
CviJI RGCY 9 cut(s) 57, 61, 90, 143, 177, 197, 289, 418, 553
CviKI_1 RGCY 9 cut(s) 57, 61, 90, 143, 177, 197, 289, 418, 553
DdeI CTNAG 2 cut(s) 135, 606
DpnI GATC 4 cut(s) 37, 129, 249, 540
DpnII GATC 4 cut(s) 35, 127, 247, 538
DrdI GACNNNNNNGTC 2 cut(s) 107, 301
DseDI GACNNNNNNGTC 2 cut(s) 107, 301
EaeI YGGCCR 1 cut(s) 88
Eam1104I CTCTTC 2 cut(s) 313, 327
EarI CTCTTC 2 cut(s) 313, 327
Eco88I CYCGRG 2 cut(s) 181, 290
EcoRII CCWGG 2 cut(s) 197, 257
FaeI CATG 3 cut(s) 95, 106, 443
FaiI YATR 8 cut(s) 93, 104, 387, 429, 441, 480, 579, 581
FaqI GGGAC 1 cut(s) 579
FatI CATG 3 cut(s) 91, 102, 439
FauI CCCGC 1 cut(s) 336
FauNDI CATATG 1 cut(s) 579
Fnu4HI GCNGC 4 cut(s) 5, 84, 191, 341
FokI GGATG 4 cut(s) 365, 387, 479, 503
Fsp4HI GCNGC 4 cut(s) 5, 84, 191, 341
FspBI CTAG 1 cut(s) 497
GlaI GCGC 1 cut(s) 369
GluI GCNGC 4 cut(s) 5, 84, 191, 341
GsuI CTGGAG 1 cut(s) 392
HaeII RGCGCY 1 cut(s) 371
HaeIII GGCC 3 cut(s) 57, 90, 197
HapII CCGG 1 cut(s) 58
HhaI GCGC 1 cut(s) 370
Hin1II CATG 3 cut(s) 95, 106, 443
Hin6I GCGC 1 cut(s) 368
HinP1I GCGC 1 cut(s) 368
HindIII AAGCTT 1 cut(s) 551
HinfI GANTC 1 cut(s) 272
HpaII CCGG 1 cut(s) 58
HphI GGTGA 2 cut(s) 30, 184
Hpy188I TCNGA 1 cut(s) 150
Hpy188III TCNNGA 4 cut(s) 226, 292, 353, 559
HpyAV CCTTC 4 cut(s) 133, 513, 525, 556
HpyCH4III ACNGT 2 cut(s) 74, 221
HpyCH4V TGCA 1 cut(s) 340
HpyF10VI GCNNNNNNNGC 1 cut(s) 415
HpyF3I CTNAG 2 cut(s) 135, 606
Hsp92II CATG 3 cut(s) 95, 106, 443
HspAI GCGC 1 cut(s) 368
KroI GCCGGC 1 cut(s) 57
KroNI GCCGGC 1 cut(s) 59
Kzo9I GATC 4 cut(s) 35, 127, 247, 538
LmnI GCTCC 2 cut(s) 373, 414
LpnPI CCDG 9 cut(s) 71, 72, 179, 184, 211, 244, 271, 356, 544
Lsp1109I GCAGC 3 cut(s) 70, 177, 352
MaeI CTAG 1 cut(s) 497
MaeIII GTNAC 1 cut(s) 353
MalI GATC 4 cut(s) 37, 129, 249, 540
MboI GATC 4 cut(s) 35, 127, 247, 538
MboII GAAGA 3 cut(s) 300, 344, 498
MhlI GDGCHC 1 cut(s) 378
MlsI TGGCCA 1 cut(s) 90
MluNI TGGCCA 1 cut(s) 90
MmeI TCCRAC 1 cut(s) 406
MnlI CCTC 9 cut(s) 144, 153, 177, 316, 340, 415, 586, 603, 632
Mox20I TGGCCA 1 cut(s) 90
MroNI GCCGGC 1 cut(s) 57
MroXI GAANNNNTTC 1 cut(s) 554
MscI TGGCCA 1 cut(s) 90
MseI TTAA 1 cut(s) 459
MslI CAYNNNNRTG 2 cut(s) 390, 461
Msp20I TGGCCA 1 cut(s) 90
MspA1I CMGCKG 1 cut(s) 343
MspI CCGG 1 cut(s) 58
MspR9I CCNGG 2 cut(s) 199, 259
MvaI CCWGG 2 cut(s) 199, 259
MwoI GCNNNNNNNGC 1 cut(s) 415
NaeI GCCGGC 1 cut(s) 59
NdeI CATATG 1 cut(s) 579
NdeII GATC 4 cut(s) 35, 127, 247, 538
NgoMIV GCCGGC 1 cut(s) 57
NlaIII CATG 3 cut(s) 95, 106, 443
NlaIV GGNNCC 1 cut(s) 437
NmuCI GTSAC 1 cut(s) 353
PaeR7I CTCGAG 1 cut(s) 290
PdiI GCCGGC 1 cut(s) 59
PdmI GAANNNNTTC 1 cut(s) 554
PfeI GAWTC 1 cut(s) 272
PkrI GCNGC 4 cut(s) 6, 85, 192, 342
PshBI ATTAAT 1 cut(s) 459
Psp6I CCWGG 2 cut(s) 197, 257
PspGI CCWGG 2 cut(s) 197, 257
PspN4I GGNNCC 1 cut(s) 437
RseI CAYNNNNRTG 2 cut(s) 390, 461
SaqAI TTAA 1 cut(s) 459
SatI GCNGC 4 cut(s) 5, 84, 191, 341
Sau3AI GATC 4 cut(s) 35, 127, 247, 538
ScrFI CCNGG 2 cut(s) 199, 259
SduI GDGCHC 1 cut(s) 378
SetI ASST 6 cut(s) 43, 351, 360, 420, 498, 555
Sfr274I CTCGAG 1 cut(s) 290
SlaI CTCGAG 1 cut(s) 290
SmiMI CAYNNNNRTG 2 cut(s) 390, 461
SmlI CTYRAG 2 cut(s) 290, 421
SmoI CTYRAG 2 cut(s) 290, 421
SsiI CCGC 2 cut(s) 5, 343
SspMI CTAG 1 cut(s) 497
StyD4I CCNGG 2 cut(s) 197, 257
TaaI ACNGT 2 cut(s) 74, 221
TaqI TCGA 4 cut(s) 126, 213, 291, 645
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 1 cut(s) 272
Tru1I TTAA 1 cut(s) 459
Tru9I TTAA 1 cut(s) 459
TscAI CASTG 1 cut(s) 527
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 3 cut(s) 83, 190, 340
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 4 cut(s) 108, 345, 459, 606
TspRI CASTG 1 cut(s) 527
VspI ATTAAT 1 cut(s) 459
XhoI CTCGAG 1 cut(s) 290
XmnI GAANNNNTTC 1 cut(s) 554
XspI CTAG 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.