RLG00000016919

respiratory chain complex III assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0019782
Physical Location & Seq
Reverse (-)
11226492 .. 11227156
665 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016919

Sequence Viewer

Length: 393 bp
ATGGCGATGGAGCCCGAGCTTAAGAGAACAGTTATAGAGGATCTCGATAGGTTTGTGAGGAGGAAGAATTTTTATACGAAGGTTGGGAAGGCTTGGAAAAGAGGTTACTTGTTGTATGGTCCACCTGGTACTGGCAAATCCAGCTTGATTGCAGCCATGGCGAATTATCTCAAGTTTGATGTTTATGATTTGGAGCTTGCTTCTGTCCGTGGCAACTCTCGATTGAGGAATATGGTCTGTGGCCGAGCTGTGGCCTGTGGAGATGAGAGAATCATAGTGTTCACCACCAACCATAAGGATGATAAGCTAGACGCGTTGTTGCGCCCTGGTCGAATGGACTTGCACATTCACTTGTCATTGCACTCCGAGTGGGTTCAAAACCTTGGCCTCTAA

Protein Analysis

131

Amino Acids

14.87

Weight (kDa)

9.51

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 36 - 76 2.4e-07 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019782)

Species Orthologous Gene IDs
malus_domestica MD05G1250700.v1.1
rosa_chinensis RchiOBHm_Chr2g0099881
rosa_laevigata RLG00000016919
rosa_rugosa Rorug02G0083700
rosa_samantha Rh2BG136100 Rh2CG137800 Rh2DG138200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 314
AclWI GGATC 1 cut(s) 48
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 1 cut(s) 67
AfaI GTAC 1 cut(s) 130
AfiI CCNNNNNNNGG 2 cut(s) 131, 250
AflII CTTAAG 1 cut(s) 20
AflIII ACRYGT 1 cut(s) 312
AgsI TTSAA 1 cut(s) 377
AjnI CCWGG 2 cut(s) 124, 325
AluBI AGCT 5 cut(s) 19, 144, 196, 248, 307
AluI AGCT 5 cut(s) 19, 144, 196, 248, 307
AlwI GGATC 1 cut(s) 48
Ama87I CYCGRG 1 cut(s) 14
AoxI GGCC 3 cut(s) 241, 252, 385
ApeKI GCWGC 1 cut(s) 152
ApoI RAATTY 1 cut(s) 67
AspLEI GCGC 1 cut(s) 324
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 1 cut(s) 274
AvaI CYCGRG 1 cut(s) 14
AvaII GGWCC 1 cut(s) 119
BanII GRGCYC 1 cut(s) 15
BbvI GCAGC 1 cut(s) 164
BciT130I CCWGG 2 cut(s) 126, 327
BfaI CTAG 1 cut(s) 308
BfrI CTTAAG 1 cut(s) 20
BisI GCNGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 154
Bme1390I CCNGG 2 cut(s) 126, 327
Bme18I GGWCC 1 cut(s) 119
BmeT110I CYCGRG 1 cut(s) 14
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 12
BmrFI CCNGG 2 cut(s) 126, 327
BpuEI CTTGAG 1 cut(s) 155
BsaJI CCNNGG 4 cut(s) 156, 208, 325, 382
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 250
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 1 cut(s) 356
BseBI CCWGG 2 cut(s) 126, 327
BseDI CCNNGG 4 cut(s) 156, 208, 325, 382
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 2 cut(s) 131, 250
BseMI GCAATG 1 cut(s) 356
BseNI ACTGG 1 cut(s) 136
BseRI GAGGAG 1 cut(s) 73
BseXI GCAGC 1 cut(s) 164
Bsh1236I CGCG 1 cut(s) 314
BshFI GGCC 3 cut(s) 243, 254, 387
BsiHKCI CYCGRG 1 cut(s) 14
BslI CCNNNNNNNGG 2 cut(s) 131, 250
BsnI GGCC 3 cut(s) 243, 254, 387
BsoBI CYCGRG 1 cut(s) 14
Bsp1286I GDGCHC 1 cut(s) 15
Bsp143I GATC 1 cut(s) 40
Bsp19I CCATGG 1 cut(s) 156
BspANI GGCC 3 cut(s) 243, 254, 387
BspFNI CGCG 1 cut(s) 314
BspLI GGNNCC 1 cut(s) 12
BspPI GGATC 1 cut(s) 48
BspTI CTTAAG 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 356
BsrI ACTGG 1 cut(s) 136
BssECI CCNNGG 4 cut(s) 156, 208, 325, 382
BssMI GATC 1 cut(s) 40
BssT1I CCWWGG 2 cut(s) 156, 382
Bst2UI CCWGG 2 cut(s) 126, 327
Bst4CI ACNGT 1 cut(s) 31
BstAFI CTTAAG 1 cut(s) 20
BstC8I GCNNGC 1 cut(s) 198
BstDSI CCRYGG 2 cut(s) 156, 208
BstF5I GGATG 1 cut(s) 304
BstFNI CGCG 1 cut(s) 314
BstHHI GCGC 1 cut(s) 324
BstKTI GATC 1 cut(s) 43
BstMBI GATC 1 cut(s) 40
BstMWI GCNNNNNNNGC 2 cut(s) 141, 158
BstNI CCWGG 2 cut(s) 126, 327
BstSCI CCNGG 2 cut(s) 124, 325
BstUI CGCG 1 cut(s) 314
BstV1I GCAGC 1 cut(s) 164
BstX2I RGATCY 1 cut(s) 40
BstYI RGATCY 1 cut(s) 40
BsuRI GGCC 3 cut(s) 243, 254, 387
BtgI CCRYGG 2 cut(s) 156, 208
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 1 cut(s) 304
Cac8I GCNNGC 1 cut(s) 198
CfoI GCGC 1 cut(s) 324
Cfr13I GGNCC 1 cut(s) 119
CseI GACGC 1 cut(s) 320
CsiI ACCWGGT 1 cut(s) 124
Csp6I GTAC 1 cut(s) 129
CviAII CATG 1 cut(s) 157
CviQI GTAC 1 cut(s) 129
DpnI GATC 1 cut(s) 42
DpnII GATC 1 cut(s) 40
EaeI YGGCCR 1 cut(s) 241
Eco130I CCWWGG 2 cut(s) 156, 382
Eco24I GRGCYC 1 cut(s) 15
Eco47I GGWCC 1 cut(s) 119
Eco88I CYCGRG 1 cut(s) 14
EcoRII CCWGG 2 cut(s) 124, 325
EcoT14I CCWWGG 2 cut(s) 156, 382
EcoT38I GRGCYC 1 cut(s) 15
ErhI CCWWGG 2 cut(s) 156, 382
FaeI CATG 1 cut(s) 160
FaiI YATR 8 cut(s) 35, 75, 117, 158, 186, 233, 275, 294
FatI CATG 1 cut(s) 156
Fnu4HI GCNGC 1 cut(s) 153
FokI GGATG 1 cut(s) 311
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 1 cut(s) 153
FspBI CTAG 1 cut(s) 308
GlaI GCGC 1 cut(s) 323
GluI GCNGC 1 cut(s) 153
HaeIII GGCC 3 cut(s) 243, 254, 387
HgaI GACGC 1 cut(s) 320
HhaI GCGC 1 cut(s) 324
Hin1II CATG 1 cut(s) 160
Hin6I GCGC 1 cut(s) 322
HinP1I GCGC 1 cut(s) 322
HinfI GANTC 1 cut(s) 270
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 2 cut(s) 122, 282
Hpy188I TCNGA 1 cut(s) 367
Hpy188III TCNNGA 2 cut(s) 44, 219
Hpy8I GTNNAC 2 cut(s) 122, 282
HpyAV CCTTC 2 cut(s) 73, 82
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4V TGCA 3 cut(s) 152, 343, 361
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 158
Hsp92II CATG 1 cut(s) 160
HspAI GCGC 1 cut(s) 322
Kzo9I GATC 1 cut(s) 40
LmnI GCTCC 2 cut(s) 10, 193
LpnPI CCDG 7 cut(s) 111, 117, 138, 154, 268, 312, 339
Lsp1109I GCAGC 1 cut(s) 164
MabI ACCWGGT 1 cut(s) 124
MaeI CTAG 1 cut(s) 308
MaeIII GTNAC 1 cut(s) 104
MalI GATC 1 cut(s) 42
MboI GATC 1 cut(s) 40
MboII GAAGA 1 cut(s) 76
MflI RGATCY 1 cut(s) 40
MhlI GDGCHC 1 cut(s) 15
MluCI AATT 2 cut(s) 67, 163
MluI ACGCGT 1 cut(s) 312
MnlI CCTC 5 cut(s) 31, 51, 54, 95, 219
MseI TTAA 1 cut(s) 21
MslI CAYNNNNRTG 1 cut(s) 297
MspCI CTTAAG 1 cut(s) 20
MspR9I CCNGG 2 cut(s) 126, 327
MvaI CCWGG 2 cut(s) 126, 327
MvnI CGCG 1 cut(s) 314
MwoI GCNNNNNNNGC 2 cut(s) 141, 158
NcoI CCATGG 1 cut(s) 156
NdeII GATC 1 cut(s) 40
NlaIII CATG 1 cut(s) 160
NlaIV GGNNCC 1 cut(s) 12
NmeAIII GCCGAG 1 cut(s) 269
PfeI GAWTC 1 cut(s) 270
PkrI GCNGC 1 cut(s) 154
Psp6I CCWGG 2 cut(s) 124, 325
PspGI CCWGG 2 cut(s) 124, 325
PspN4I GGNNCC 1 cut(s) 12
PspPI GGNCC 1 cut(s) 119
PsuI RGATCY 1 cut(s) 40
RsaI GTAC 1 cut(s) 130
RsaNI GTAC 1 cut(s) 129
RseI CAYNNNNRTG 1 cut(s) 297
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 1 cut(s) 153
Sau3AI GATC 1 cut(s) 40
Sau96I GGNCC 1 cut(s) 119
ScrFI CCNGG 2 cut(s) 126, 327
SduI GDGCHC 1 cut(s) 15
SexAI ACCWGGT 1 cut(s) 124
SinI GGWCC 1 cut(s) 119
SmiMI CAYNNNNRTG 1 cut(s) 297
SmlI CTYRAG 2 cut(s) 20, 170
SmoI CTYRAG 2 cut(s) 20, 170
Sse9I AATT 2 cut(s) 67, 163
SspMI CTAG 1 cut(s) 308
StyD4I CCNGG 2 cut(s) 124, 325
StyI CCWWGG 2 cut(s) 156, 382
TaaI ACNGT 1 cut(s) 31
TaqI TCGA 3 cut(s) 45, 220, 331
TasI AATT 2 cut(s) 67, 163
TfiI GAWTC 1 cut(s) 270
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TseI GCWGC 1 cut(s) 152
TspGWI ACGGA 1 cut(s) 197
Vha464I CTTAAG 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 119
XapI RAATTY 1 cut(s) 67
XspI CTAG 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.