Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0008499

Physical Location & Seq

Genomic Coordinates Forward (+)

31271993 .. 31274921

Gene Structure 2929 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018520

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1917 bp

ATGAGCTCGGTTGGTATGGCAGAAGATGCTGACGTATATGCTGAACCTCCTGATCCCGATGTTTTTGAAACTGATCCAACATGTCGTTATCTTCGGTATAAAGATGTGCTCGGGAAAGGTGCCTTCAAGACAGTATACAAAGCATTTGATGAAGTGAATGGACTTGAAGTAGCATGGAACCAAGTTAGAATTGATGAAGTGCTACAATCACCGGGTGACCTGGAGCGGCTGTATTCAGAAGTTCATCTTTTGAAATCATTGAAGCACAAAAATATAGTAAAATTCTATAATTCCTGGATCGATGATAAGAACAAGACAGTTAATATAATAACGGAGTTATTCACTTCGGGGAGTCTCAGACAGTACTGTAAGAAACACAAGAAAGTGGACAAGAAAGCTGTAAAGGGATGGGCAAGACAAATATTGATGGGATTGAGCTATCTCCATAGTCACAACCCACCAATTATGCATAGGGACTTGAAGTGTGATAATATATTTATCAATGGTAACCAAGGAGAAGTTAAAATTGGAGATCTTGGGTTGGCAACTGTCATGGAGCAAGCTACTGCCAAAAGTGTAATCGGAACCCCTGAGTTTATGGCTCCTGAGCTCTATGATGAAAACTATAATGAGTTAGCAGATATATACTCCTTTGGGATGTGCATGCTAGAGATGGTGACTAATGAATACCCTTATAGTGAATGTAGAAACTCAGCTCAAATATATAAGAAGGTTTCGTCTGGAATAAAACCCGCTGCCCTTTCCAAGGTAAAAGATCCTGAAATGAAACAATTTATTGAGAAGTGTTTAGTCCCAGCATCCGAAAGGTTGTCAGCAGAAGAGCTTCTGATGGACCCTTTCCTCCAATTGAATGGCTCGGTAAAAAATCGTCCTCTGCCACTTCCAGATATCGTATTGCCAAAAATGGGAGCCTTTGGAGATCGTTGTGTGATGTCGGAAGGACCTTTAAGCTCACGGAACAATGCCTTATCTAAGGATTTTGATGATGGGGAGCCACCAGTGATAAACTTTTTTAGCAACTCTGGTGACGGCGAGTCCCGTTCCTTTTACGTGGAGGTTCAGAGGTCAAAAAGAGGCAACTTTTTCTTCTTAAAAGGCGAGCATAATGACGAAAACTCCATATCGTTAATACTGAGAATAGCTGATCAGAATGGTCGTGCAAGGAACATCCATTTCTTATTCTATCTCGACAGTGATACAGCACTTTCAGTTTCAAGTGAAATGGTTGAGCAACTCGAACTAGAAGATCATAATGTTATCTTCATAGCAGAGCTTATTGATTTGTTATTGATGAACTTGATATCAGAATGGAAGTGTTGTGTCCGTGTTGATCATTTGGTTGCTCAAAACAGAAAGAATGTGAACCATCAGAAAGACTCTCAGTCGCCAAAGCCTCAGGAATGCTCGGGCAGATGTCACCAGAATGTTTGTGAGGATGCCAATTCCTCAAAACCACCGATTTGCCCAACTTCTTCCACTTTTGAAGGCTCCATTCCTCCACCAATACTAGAGAAGCCTCCTAATTATGTGAAGGGTGTTCCGAGTTCTAATGCGGTTTTAGCTAGTTCAACCACCACAGTAGAACGGTATTATGAGATGTCATGTGCTTCTATGACCCCTAATGACGCTACACATCGATCTCATAATTCATATGCATCCGCAGAATCTGGGTCTTTGGACTTAAATTTTCATTTGCCAAATGGCTACAAAAGTGCAAGTTGTCCAATCAGTGTTTCCTCATTCTGCACTGCAGACGACTTTGAGTTTAGAAAGGAGTTGGAAACGATTGAACTGCAATTCCAGCTGGAGCTTCAAGATATATCGAAGAGAAGACATGAAGCTATCAAGGAAACAAGAAAAAGGTTATCCCAGAAAGATATTGAGTTGGTTCGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

639

Amino Acids

71.99

Weight (kDa)

5.58

Isoelectric Point (pI)

49.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	32 - 279	3e-37	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	34 - 288	1.5e-54	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008499)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49160 AT1G49160 AT1G49160 AT1G49160 AT1G49160 AT1G49160 AT1G49160 AT3G18750 AT3G18750 AT3G18750 AT3G18750 AT3G18750 AT3G18750
fragaria_vesca	FvH4_1g23971
rosa_chinensis	RchiOBHm_Chr2g0120101
rosa_laevigata	RLG00000018520
rosa_multiflora	Rmu_sc0010863.1_g000002
rosa_roxburghii	Rroxscaffold_2G00123730
rosa_rugosa	Rorug02G0226800
rosa_samantha	Rh2AG283300 Rh2BG294600 Rh2DG308300
rosa_wichuraiana	Rw2G022690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	119
AccBSI	CCGCTC	1 cut(s)	226
AccI	GTMKAC	1 cut(s)	135
AciI	CCGC	4 cut(s)	226, 753, 1574, 1680
AclWI	GGATC	4 cut(s)	47, 68, 305, 770
AcsI	RAATTY	2 cut(s)	281, 1705
AdeI	CACNNNGTG	1 cut(s)	215
AfaI	GTAC	1 cut(s)	365
AfiI	CCNNNNNNNGG	2 cut(s)	766, 926
AflIII	ACRYGT	1 cut(s)	80
AhdI	GACNNNNNGTC	2 cut(s)	1054, 1402
AjnI	CCWGG	2 cut(s)	219, 293
AjuI	GAANNNNNNNTTGG	4 cut(s)	510, 542, 1738, 1770
AloI	GAACNNNNNNTCC	2 cut(s)	1803, 1835
Alw21I	GWGCWC	3 cut(s)	8, 111, 612
Alw26I	GTCTC	1 cut(s)	359
AlwI	GGATC	4 cut(s)	47, 68, 305, 770
AlwNI	CAGNNNCTG	1 cut(s)	1688
Ama87I	CYCGRG	2 cut(s)	110, 1426
ApeKI	GCWGC	1 cut(s)	755
ApoI	RAATTY	2 cut(s)	281, 1705
Asp700I	GAANNNNTTC	1 cut(s)	843
AspS9I	GGNCC	2 cut(s)	853, 962
AsuC2I	CCSGG	1 cut(s)	213
AsuHPI	GGTGA	5 cut(s)	201, 227, 688, 1058, 1430
AvaI	CYCGRG	2 cut(s)	110, 1426
AvaII	GGWCC	2 cut(s)	853, 962
AxyI	CCTNAGG	1 cut(s)	1416
BanI	GGYRCC	1 cut(s)	119
BanII	GRGCYC	2 cut(s)	8, 612
BbsI	GAAGAC	1 cut(s)	1858
Bbv12I	GWGCWC	3 cut(s)	8, 111, 612
BbvI	GCAGC	1 cut(s)	742
BccI	CCATC	6 cut(s)	402, 421, 667, 844, 1001, 1395
BceAI	ACGGC	1 cut(s)	1066
BcgI	CGANNNNNNTGC	2 cut(s)	1795, 1829
BciT130I	CCWGG	2 cut(s)	221, 295
BclI	TGATCA	2 cut(s)	1165, 1351
BcnI	CCSGG	1 cut(s)	213
BcoDI	GTCTC	1 cut(s)	359
BfaI	CTAG	5 cut(s)	668, 1262, 1529, 1584, 1915
BfmI	CTRYAG	1 cut(s)	1770
BglII	AGATCT	1 cut(s)	532
BisI	GCNGC	2 cut(s)	227, 756
BlsI	GCNGC	2 cut(s)	228, 757
BmcAI	AGTACT	1 cut(s)	365
Bme1390I	CCNGG	3 cut(s)	213, 221, 295
Bme18I	GGWCC	2 cut(s)	853, 962
BmeRI	GACNNNNNGTC	2 cut(s)	1054, 1402
BmeT110I	CYCGRG	2 cut(s)	110, 1426
BmgT120I	GGNCC	2 cut(s)	853, 962
BmiI	GGNNCC	8 cut(s)	121, 179, 586, 603, 855, 931, 1014, 1510
BmrFI	CCNGG	3 cut(s)	213, 221, 295
BmsI	GCATC	4 cut(s)	16, 827, 1447, 1685
BpiI	GAAGAC	1 cut(s)	1858
BpmI	CTGGAG	2 cut(s)	242, 1847
Bpu10I	CCTNAGC	1 cut(s)	606
BpuMI	CCSGG	1 cut(s)	213
Bsa29I	ATCGAT	2 cut(s)	300, 1657
BsaAI	YACGTR	1 cut(s)	1072
BsaJI	CCNNGG	2 cut(s)	511, 765
BsaXI	ACNNNNNCTCC	4 cut(s)	215, 245, 1121, 1151
Bsc4I	CCNNNNNNNGG	2 cut(s)	766, 926
Bse1I	ACTGG	1 cut(s)	1019
Bse21I	CCTNAGG	1 cut(s)	1416
BseBI	CCWGG	2 cut(s)	221, 295
BseCI	ATCGAT	2 cut(s)	300, 1657
BseDI	CCNNGG	2 cut(s)	511, 765
BseGI	GGATG	6 cut(s)	413, 663, 818, 1188, 1462, 1676
BseLI	CCNNNNNNNGG	2 cut(s)	766, 926
BseMII	CTCAG	7 cut(s)	370, 582, 597, 726, 1145, 1415, 1430
BseNI	ACTGG	1 cut(s)	1019
BseXI	GCAGC	1 cut(s)	742
BseYI	CCCAGC	1 cut(s)	814
BsgI	GTGCAG	1 cut(s)	1750
BshNI	GGYRCC	1 cut(s)	119
BshVI	ATCGAT	2 cut(s)	300, 1657
BsiHKAI	GWGCWC	3 cut(s)	8, 111, 612
BsiHKCI	CYCGRG	2 cut(s)	110, 1426
BsiSI	CCGG	1 cut(s)	212
BslFI	GGGAC	3 cut(s)	488, 797, 1042
BslI	CCNNNNNNNGG	2 cut(s)	766, 926
BsmAI	GTCTC	1 cut(s)	359
BsmFI	GGGAC	3 cut(s)	488, 797, 1042
BsmI	GAATGC	1 cut(s)	1427
BsoBI	CYCGRG	2 cut(s)	110, 1426
Bsp1286I	GDGCHC	3 cut(s)	8, 111, 612
BspACI	CCGC	4 cut(s)	226, 753, 1574, 1680
BspCNI	CTCAG	7 cut(s)	369, 583, 598, 725, 1146, 1414, 1429
BspDI	ATCGAT	2 cut(s)	300, 1657
BspLI	GGNNCC	8 cut(s)	121, 179, 586, 603, 855, 931, 1014, 1510
BspMAI	CTGCAG	1 cut(s)	1774
BspPI	GGATC	4 cut(s)	47, 68, 305, 770
BspQI	GCTCTTC	1 cut(s)	834
BspT107I	GGYRCC	1 cut(s)	119
BsrBI	CCGCTC	1 cut(s)	226
BsrI	ACTGG	1 cut(s)	1019
BssECI	CCNNGG	2 cut(s)	511, 765
BssNAI	GTATAC	1 cut(s)	136
BssT1I	CCWWGG	2 cut(s)	511, 765
Bst1107I	GTATAC	1 cut(s)	136
Bst2UI	CCWGG	2 cut(s)	221, 295
Bst4CI	ACNGT	8 cut(s)	133, 319, 363, 368, 550, 1214, 1600, 1608
Bst6I	CTCTTC	2 cut(s)	834, 1841
BstAPI	GCANNNNNTGC	1 cut(s)	26
BstBAI	YACGTR	1 cut(s)	1072
BstC8I	GCNNGC	3 cut(s)	561, 665, 1121
BstDEI	CTNAG	8 cut(s)	356, 591, 606, 712, 993, 1154, 1401, 1416
BstEII	GGTNACC	2 cut(s)	215, 506
BstENI	CCTNNNNNAGG	1 cut(s)	764
BstF5I	GGATG	6 cut(s)	413, 663, 818, 1188, 1462, 1676
BstMAI	GTCTC	1 cut(s)	359
BstMWI	GCNNNNNNNGC	3 cut(s)	26, 1580, 1822
BstNI	CCWGG	2 cut(s)	221, 295
BstNSI	RCATGY	2 cut(s)	84, 667
BstPI	GGTNACC	2 cut(s)	215, 506
BstSCI	CCNGG	3 cut(s)	211, 219, 293
BstSFI	CTRYAG	1 cut(s)	1770
BstV1I	GCAGC	1 cut(s)	742
BstV2I	GAAGAC	1 cut(s)	1858
BstX2I	RGATCY	2 cut(s)	532, 775
BstXI	CCANNNNNNTGG	1 cut(s)	872
BstYI	RGATCY	2 cut(s)	532, 775
BstZ17I	GTATAC	1 cut(s)	136
Bsu15I	ATCGAT	2 cut(s)	300, 1657
Bsu36I	CCTNAGG	1 cut(s)	1416
BsuTUI	ATCGAT	2 cut(s)	300, 1657
BtsCI	GGATG	6 cut(s)	413, 663, 818, 1188, 1462, 1676
BtsI	GCAGTG	1 cut(s)	1767
BtsIMutI	CAGTG	4 cut(s)	1026, 1219, 1756, 1767
Cac8I	GCNNGC	3 cut(s)	561, 665, 1121
CaiI	CAGNNNCTG	1 cut(s)	1688
Cfr13I	GGNCC	2 cut(s)	853, 962
ClaI	ATCGAT	2 cut(s)	300, 1657
CseI	GACGC	1 cut(s)	1655
Csp6I	GTAC	1 cut(s)	364
CviAII	CATG	6 cut(s)	81, 174, 553, 664, 1623, 1856
CviQI	GTAC	1 cut(s)	364
DdeI	CTNAG	8 cut(s)	356, 591, 606, 712, 993, 1154, 1401, 1416
DraIII	CACNNNGTG	1 cut(s)	215
DriI	GACNNNNNGTC	2 cut(s)	1054, 1402
Eam1104I	CTCTTC	2 cut(s)	834, 1841
Eam1105I	GACNNNNNGTC	2 cut(s)	1054, 1402
EarI	CTCTTC	2 cut(s)	834, 1841
Ecl136II	GAGCTC	2 cut(s)	6, 610
Eco130I	CCWWGG	2 cut(s)	511, 765
Eco24I	GRGCYC	2 cut(s)	8, 612
Eco32I	GATATC	2 cut(s)	910, 1323
Eco47I	GGWCC	2 cut(s)	853, 962
Eco53kI	GAGCTC	2 cut(s)	6, 610
Eco81I	CCTNAGG	1 cut(s)	1416
Eco88I	CYCGRG	2 cut(s)	110, 1426
Eco91I	GGTNACC	2 cut(s)	215, 506
EcoICRI	GAGCTC	2 cut(s)	6, 610
EcoNI	CCTNNNNNAGG	1 cut(s)	764
EcoO109I	RGGNCCY	1 cut(s)	962
EcoO65I	GGTNACC	2 cut(s)	215, 506
EcoRII	CCWGG	2 cut(s)	219, 293
EcoRV	GATATC	2 cut(s)	910, 1323
EcoT14I	CCWWGG	2 cut(s)	511, 765
EcoT22I	ATGCAT	2 cut(s)	471, 1678
EcoT38I	GRGCYC	2 cut(s)	8, 612
ErhI	CCWWGG	2 cut(s)	511, 765
FaeI	CATG	6 cut(s)	84, 177, 556, 667, 1626, 1859
FalI	AAGNNNNNCTT	2 cut(s)	231, 263
FaqI	GGGAC	3 cut(s)	488, 797, 1042
FatI	CATG	6 cut(s)	80, 173, 552, 663, 1622, 1855
FauI	CCCGC	1 cut(s)	760
FauNDI	CATATG	1 cut(s)	1672
FbaI	TGATCA	2 cut(s)	1165, 1351
FblI	GTMKAC	1 cut(s)	135
Fnu4HI	GCNGC	2 cut(s)	227, 756
FokI	GGATG	6 cut(s)	420, 670, 805, 1175, 1469, 1663
FriOI	GRGCYC	2 cut(s)	8, 612
Fsp4HI	GCNGC	2 cut(s)	227, 756
FspBI	CTAG	5 cut(s)	668, 1262, 1529, 1584, 1915
GluI	GCNGC	2 cut(s)	227, 756
GsaI	CCCAGC	1 cut(s)	818
GsuI	CTGGAG	2 cut(s)	242, 1847
HapII	CCGG	1 cut(s)	212
HgaI	GACGC	1 cut(s)	1655
Hin1II	CATG	6 cut(s)	84, 177, 556, 667, 1626, 1859
HinfI	GANTC	4 cut(s)	352, 1055, 1397, 1685
HpaII	CCGG	1 cut(s)	212
HphI	GGTGA	5 cut(s)	201, 227, 688, 1058, 1430
Hpy166II	GTNNAC	3 cut(s)	136, 388, 1384
Hpy8I	GTNNAC	3 cut(s)	136, 388, 1384
HpyAV	CCTTC	5 cut(s)	133, 724, 953, 1499, 1546
HpyCH4III	ACNGT	8 cut(s)	133, 319, 363, 368, 550, 1214, 1600, 1608
HpyCH4IV	ACGT	2 cut(s)	33, 1071
HpyCH4V	TGCA	8 cut(s)	469, 663, 1181, 1676, 1736, 1767, 1772, 1816
HpyF10VI	GCNNNNNNNGC	3 cut(s)	26, 1580, 1822
HpyF3I	CTNAG	8 cut(s)	356, 591, 606, 712, 993, 1154, 1401, 1416
HpySE526I	ACGT	2 cut(s)	33, 1071
Hsp92II	CATG	6 cut(s)	84, 177, 556, 667, 1626, 1859
Ksp22I	TGATCA	2 cut(s)	1165, 1351
LguI	GCTCTTC	1 cut(s)	834
LmnI	GCTCC	7 cut(s)	223, 556, 607, 929, 1012, 1514, 1828
Lsp1109I	GCAGC	1 cut(s)	742
LweI	GCATC	4 cut(s)	16, 827, 1447, 1685
MaeI	CTAG	5 cut(s)	668, 1262, 1529, 1584, 1915
MaeII	ACGT	2 cut(s)	33, 1071
MaeIII	GTNAC	6 cut(s)	215, 449, 506, 676, 1046, 1436
MbiI	CCGCTC	1 cut(s)	226
MboII	GAAGA	9 cut(s)	35, 83, 851, 1099, 1273, 1277, 1485, 1858, 1863
MfeI	CAATTG	1 cut(s)	866
MflI	RGATCY	2 cut(s)	532, 775
MhlI	GDGCHC	3 cut(s)	8, 111, 612
MlyI	GAGTC	3 cut(s)	361, 1064, 1391
MmeI	TCCRAC	3 cut(s)	101, 936, 1779
Mph1103I	ATGCAT	2 cut(s)	471, 1678
MroXI	GAANNNNTTC	1 cut(s)	843
MseI	TTAA	6 cut(s)	321, 522, 968, 1112, 1148, 1703
MslI	CAYNNNNRTG	1 cut(s)	1443
MspA1I	CMGCKG	2 cut(s)	755, 1825
MspI	CCGG	1 cut(s)	212
MspR9I	CCNGG	3 cut(s)	213, 221, 295
MunI	CAATTG	1 cut(s)	866
Mva1269I	GAATGC	1 cut(s)	1427
MvaI	CCWGG	2 cut(s)	221, 295
MwoI	GCNNNNNNNGC	3 cut(s)	26, 1580, 1822
NciI	CCSGG	1 cut(s)	213
NdeI	CATATG	1 cut(s)	1672
NlaIII	CATG	6 cut(s)	84, 177, 556, 667, 1626, 1859
NlaIV	GGNNCC	8 cut(s)	121, 179, 586, 603, 855, 931, 1014, 1510
NmuCI	GTSAC	5 cut(s)	215, 449, 676, 1046, 1436
NsiI	ATGCAT	2 cut(s)	471, 1678
NspI	RCATGY	2 cut(s)	84, 667
PaeI	GCATGC	1 cut(s)	667
PciI	ACATGT	1 cut(s)	80
PciSI	GCTCTTC	1 cut(s)	834
PctI	GAATGC	1 cut(s)	1427
PdmI	GAANNNNTTC	1 cut(s)	843
PfeI	GAWTC	1 cut(s)	1685
PfoI	TCCNGGA	1 cut(s)	293
PkrI	GCNGC	2 cut(s)	228, 757
PleI	GAGTC	3 cut(s)	360, 1063, 1391
PpsI	GAGTC	3 cut(s)	360, 1063, 1391
Ppu21I	YACGTR	1 cut(s)	1072
PpuMI	RGGWCCY	1 cut(s)	962
PscI	ACATGT	1 cut(s)	80
Psp124BI	GAGCTC	2 cut(s)	8, 612
Psp5II	RGGWCCY	1 cut(s)	962
Psp6I	CCWGG	2 cut(s)	219, 293
PspEI	GGTNACC	2 cut(s)	215, 506
PspFI	CCCAGC	1 cut(s)	814
PspGI	CCWGG	2 cut(s)	219, 293
PspN4I	GGNNCC	8 cut(s)	121, 179, 586, 603, 855, 931, 1014, 1510
PspPI	GGNCC	2 cut(s)	853, 962
PspPPI	RGGWCCY	1 cut(s)	962
PstI	CTGCAG	1 cut(s)	1774
PstNI	CAGNNNCTG	1 cut(s)	1688
PsuI	RGATCY	2 cut(s)	532, 775
PvuII	CAGCTG	1 cut(s)	1825
RsaI	GTAC	1 cut(s)	365
RsaNI	GTAC	1 cut(s)	364
RseI	CAYNNNNRTG	1 cut(s)	1443
SacI	GAGCTC	2 cut(s)	8, 612
SapI	GCTCTTC	1 cut(s)	834
SaqAI	TTAA	6 cut(s)	321, 522, 968, 1112, 1148, 1703
SatI	GCNGC	2 cut(s)	227, 756
Sau96I	GGNCC	2 cut(s)	853, 962
ScaI	AGTACT	1 cut(s)	365
SchI	GAGTC	3 cut(s)	361, 1064, 1391
ScrFI	CCNGG	3 cut(s)	213, 221, 295
SduI	GDGCHC	3 cut(s)	8, 111, 612
SfaNI	GCATC	4 cut(s)	16, 827, 1447, 1685
SfcI	CTRYAG	1 cut(s)	1770
SinI	GGWCC	2 cut(s)	853, 962
SmiMI	CAYNNNNRTG	1 cut(s)	1443
SphI	GCATGC	1 cut(s)	667
SsiI	CCGC	4 cut(s)	226, 753, 1574, 1680
SspI	AATATT	1 cut(s)	423
SspMI	CTAG	5 cut(s)	668, 1262, 1529, 1584, 1915
SstI	GAGCTC	2 cut(s)	8, 612
StyD4I	CCNGG	3 cut(s)	211, 219, 293
StyI	CCWWGG	2 cut(s)	511, 765
TaaI	ACNGT	8 cut(s)	133, 319, 363, 368, 550, 1214, 1600, 1608
TaiI	ACGT	2 cut(s)	36, 1074
TaqI	TCGA	5 cut(s)	300, 1209, 1257, 1657, 1844
TatI	WGTACW	1 cut(s)	363
TauI	GCSGC	1 cut(s)	229
TfiI	GAWTC	1 cut(s)	1685
Tru1I	TTAA	6 cut(s)	321, 522, 968, 1112, 1148, 1703
Tru9I	TTAA	6 cut(s)	321, 522, 968, 1112, 1148, 1703
TscAI	CASTG	4 cut(s)	1026, 1219, 1756, 1774
TseFI	GTSAC	5 cut(s)	215, 449, 676, 1046, 1436
TseI	GCWGC	1 cut(s)	755
Tsp45I	GTSAC	5 cut(s)	215, 449, 676, 1046, 1436
TspGWI	ACGGA	3 cut(s)	347, 991, 1334
TspRI	CASTG	4 cut(s)	1026, 1219, 1756, 1774
VpaK11BI	GGWCC	2 cut(s)	853, 962
XagI	CCTNNNNNAGG	1 cut(s)	764
XapI	RAATTY	2 cut(s)	281, 1705
XceI	RCATGY	2 cut(s)	84, 667
XmiI	GTMKAC	1 cut(s)	135
XmnI	GAANNNNTTC	1 cut(s)	843
XspI	CTAG	5 cut(s)	668, 1262, 1529, 1584, 1915
ZrmI	AGTACT	1 cut(s)	365
Zsp2I	ATGCAT	2 cut(s)	471, 1678

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.