RLG00000018667

tRNA (guanine(26)-N(2))-dimethyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0019066
Physical Location & Seq
Forward (+)
33549470 .. 33550747
1278 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018667

Sequence Viewer

Length: 690 bp
ATGTTTTCTACTCCTTTGAATGAGAAAGGACTATTTGTGCCATGCTGCAGCAGCACCACCATGCTTGCTTTGCAATACCACATGCACAAACGTAAATTGCAAGTTAACCATATCTTGACCATAGCACCAAATCTTCAATATTCTCGGTCATCATCTCAGCATCCTTCATCATCTCTTGGCTATAGGATTTTTGGGGATTTTTATTTTGATTTTCCATTTCACTTGGGAGGGTTTAGACATGTCAACTTTAGCAAAGGAGTTGTTCATCTTGTGATAGATGATGACTTATTTCTTTATCTGTCTCAGGTGGCAAGCCGTGCAAAAGTAAATTCACCAGCACTCAAAGCAGTGGTGTCTACCCTTCAAAAGGAGTATCATGGGATCATACGAGCCTTAACATTTTGTCTCGGTCCAAAGCCTAATACTCCAATCGGTTATATTCTTTTGCAAGAGGGATATGCTGTGAGTAGGTCACATATCGAGTCCAATGCACTCAAGACCAACTGTCCAATGTCAGAATGCATTAGAATTGTCCAAGAACTACAGCAGTGGTACAGTGCTGAACGAATTCACTCCCAGAACATTGGCCAGTTTAAGCTGCATCTAATTATGCTAAGCATTGAGGAGTTCATGCTCAATGAGAGAAGAACTGTCGTCCCTCTCAGGCAAGAATGTGAGCTTATGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRM

Protein Analysis

230

Amino Acids

26.22

Weight (kDa)

8.89

Isoelectric Point (pI)

68.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 356
AclWI GGATC 1 cut(s) 389
AcoI YGGCCR 1 cut(s) 586
AcsI RAATTY 2 cut(s) 328, 567
AfaI GTAC 1 cut(s) 554
AfiI CCNNNNNNNGG 1 cut(s) 367
AflIII ACRYGT 1 cut(s) 238
AgsI TTSAA 3 cut(s) 19, 137, 365
AhdI GACNNNNNGTC 1 cut(s) 504
AluBI AGCT 2 cut(s) 598, 679
AluI AGCT 2 cut(s) 598, 679
Alw26I GTCTC 2 cut(s) 306, 410
AlwI GGATC 1 cut(s) 389
AoxI GGCC 1 cut(s) 586
ApeKI GCWGC 4 cut(s) 45, 48, 51, 598
ApoI RAATTY 2 cut(s) 328, 567
Asp700I GAANNNNTTC 1 cut(s) 567
AspS9I GGNCC 1 cut(s) 410
AsuHPI GGTGA 1 cut(s) 324
AvaII GGWCC 1 cut(s) 410
BalI TGGCCA 1 cut(s) 588
BbvI GCAGC 4 cut(s) 32, 60, 63, 585
BceAI ACGGC 1 cut(s) 300
BcgI CGANNNNNNTGC 2 cut(s) 470, 504
BcoDI GTCTC 2 cut(s) 306, 410
BfmI CTRYAG 3 cut(s) 46, 181, 542
BisI GCNGC 4 cut(s) 46, 49, 52, 599
BlpI GCTNAGC 1 cut(s) 614
BlsI GCNGC 4 cut(s) 47, 50, 53, 600
Bme18I GGWCC 1 cut(s) 410
BmeRI GACNNNNNGTC 1 cut(s) 504
BmgT120I GGNCC 1 cut(s) 410
BmsI GCATC 2 cut(s) 169, 610
Bpu1102I GCTNAGC 1 cut(s) 614
BpuEI CTTGAG 1 cut(s) 479
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse1I ACTGG 1 cut(s) 589
BseGI GGATG 1 cut(s) 160
BseLI CCNNNNNNNGG 1 cut(s) 367
BseMII CTCAG 3 cut(s) 170, 317, 676
BseNI ACTGG 1 cut(s) 589
BseRI GAGGAG 1 cut(s) 638
BseXI GCAGC 4 cut(s) 32, 60, 63, 585
BshFI GGCC 1 cut(s) 588
BslFI GGGAC 1 cut(s) 641
BslI CCNNNNNNNGG 1 cut(s) 367
BsmAI GTCTC 2 cut(s) 306, 410
BsmFI GGGAC 1 cut(s) 641
BsmI GAATGC 1 cut(s) 524
BsnI GGCC 1 cut(s) 588
Bsp143I GATC 1 cut(s) 381
Bsp1720I GCTNAGC 1 cut(s) 614
BspANI GGCC 1 cut(s) 588
BspCNI CTCAG 3 cut(s) 169, 316, 675
BspMAI CTGCAG 1 cut(s) 50
BspPI GGATC 1 cut(s) 389
BsrI ACTGG 1 cut(s) 589
BssMI GATC 1 cut(s) 381
Bst4CI ACNGT 3 cut(s) 506, 557, 652
BstAPI GCANNNNNTGC 1 cut(s) 317
BstC8I GCNNGC 2 cut(s) 66, 313
BstDEI CTNAG 4 cut(s) 156, 303, 614, 662
BstENI CCTNNNNNAGG 1 cut(s) 365
BstF5I GGATG 1 cut(s) 160
BstKTI GATC 1 cut(s) 384
BstMAI GTCTC 2 cut(s) 306, 410
BstMBI GATC 1 cut(s) 381
BstMWI GCNNNNNNNGC 4 cut(s) 51, 70, 317, 344
BstNSI RCATGY 2 cut(s) 85, 242
BstSFI CTRYAG 3 cut(s) 46, 181, 542
BstV1I GCAGC 4 cut(s) 32, 60, 63, 585
BstXI CCANNNNNNTGG 1 cut(s) 584
BsuRI GGCC 1 cut(s) 588
BtsCI GGATG 1 cut(s) 160
BtsI GCAGTG 2 cut(s) 354, 554
BtsIMutI CAGTG 3 cut(s) 354, 554, 562
Cac8I GCNNGC 2 cut(s) 66, 313
Cfr13I GGNCC 1 cut(s) 410
Csp6I GTAC 1 cut(s) 553
CspCI CAANNNNNGTGG 2 cut(s) 46, 81
CviAII CATG 6 cut(s) 42, 61, 82, 239, 377, 631
CviJI RGCY 7 cut(s) 180, 315, 392, 418, 588, 598, 679
CviKI_1 RGCY 7 cut(s) 180, 315, 392, 418, 588, 598, 679
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 4 cut(s) 156, 303, 614, 662
DpnI GATC 1 cut(s) 383
DpnII GATC 1 cut(s) 381
DriI GACNNNNNGTC 1 cut(s) 504
EaeI YGGCCR 1 cut(s) 586
Eam1105I GACNNNNNGTC 1 cut(s) 504
Eco47I GGWCC 1 cut(s) 410
EcoNI CCTNNNNNAGG 1 cut(s) 365
EcoRI GAATTC 1 cut(s) 567
EcoT22I ATGCAT 1 cut(s) 524
FaeI CATG 6 cut(s) 45, 64, 85, 242, 380, 634
FaqI GGGAC 1 cut(s) 641
FatI CATG 6 cut(s) 41, 60, 81, 238, 376, 630
FblI GTMKAC 1 cut(s) 356
Fnu4HI GCNGC 4 cut(s) 46, 49, 52, 599
FokI GGATG 1 cut(s) 147
Fsp4HI GCNGC 4 cut(s) 46, 49, 52, 599
GluI GCNGC 4 cut(s) 46, 49, 52, 599
HaeIII GGCC 1 cut(s) 588
Hin1II CATG 6 cut(s) 45, 64, 85, 242, 380, 634
HincII GTYRAC 2 cut(s) 106, 244
HindII GTYRAC 2 cut(s) 106, 244
HinfI GANTC 1 cut(s) 482
HpaI GTTAAC 1 cut(s) 106
HphI GGTGA 1 cut(s) 324
Hpy166II GTNNAC 3 cut(s) 106, 244, 357
Hpy188I TCNGA 1 cut(s) 517
Hpy188III TCNNGA 2 cut(s) 115, 496
Hpy8I GTNNAC 3 cut(s) 106, 244, 357
HpyAV CCTTC 2 cut(s) 174, 371
HpyCH4III ACNGT 3 cut(s) 506, 557, 652
HpyCH4IV ACGT 1 cut(s) 91
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 70, 317, 344
HpyF3I CTNAG 4 cut(s) 156, 303, 614, 662
HpySE526I ACGT 1 cut(s) 91
Hsp92II CATG 6 cut(s) 45, 64, 85, 242, 380, 634
KspAI GTTAAC 1 cut(s) 106
Kzo9I GATC 1 cut(s) 381
LpnPI CCDG 5 cut(s) 290, 348, 590, 602, 649
Lsp1109I GCAGC 4 cut(s) 32, 60, 63, 585
LweI GCATC 2 cut(s) 169, 610
MaeII ACGT 1 cut(s) 91
MaeIII GTNAC 1 cut(s) 471
MalI GATC 1 cut(s) 383
MboI GATC 1 cut(s) 381
MboII GAAGA 2 cut(s) 125, 657
MlsI TGGCCA 1 cut(s) 588
MluCI AATT 5 cut(s) 95, 328, 528, 567, 606
MluNI TGGCCA 1 cut(s) 588
MlyI GAGTC 1 cut(s) 491
MnlI CCTC 4 cut(s) 221, 445, 616, 669
Mox20I TGGCCA 1 cut(s) 588
Mph1103I ATGCAT 1 cut(s) 524
MroXI GAANNNNTTC 1 cut(s) 567
MscI TGGCCA 1 cut(s) 588
MseI TTAA 3 cut(s) 105, 395, 594
MslI CAYNNNNRTG 1 cut(s) 59
Msp20I TGGCCA 1 cut(s) 588
Mva1269I GAATGC 1 cut(s) 524
MwoI GCNNNNNNNGC 4 cut(s) 51, 70, 317, 344
NdeII GATC 1 cut(s) 381
NlaIII CATG 6 cut(s) 45, 64, 85, 242, 380, 634
NmuCI GTSAC 1 cut(s) 471
NsiI ATGCAT 1 cut(s) 524
NspI RCATGY 2 cut(s) 85, 242
PciI ACATGT 1 cut(s) 238
PctI GAATGC 1 cut(s) 524
PdmI GAANNNNTTC 1 cut(s) 567
PkrI GCNGC 4 cut(s) 47, 50, 53, 600
PleI GAGTC 1 cut(s) 490
PpsI GAGTC 1 cut(s) 490
PscI ACATGT 1 cut(s) 238
PspPI GGNCC 1 cut(s) 410
PstI CTGCAG 1 cut(s) 50
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
RseI CAYNNNNRTG 1 cut(s) 59
SaqAI TTAA 3 cut(s) 105, 395, 594
SatI GCNGC 4 cut(s) 46, 49, 52, 599
Sau3AI GATC 1 cut(s) 381
Sau96I GGNCC 1 cut(s) 410
SchI GAGTC 1 cut(s) 491
SetI ASST 5 cut(s) 94, 309, 473, 600, 681
SfaNI GCATC 2 cut(s) 169, 610
SfcI CTRYAG 3 cut(s) 46, 181, 542
SinI GGWCC 1 cut(s) 410
SmiMI CAYNNNNRTG 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
Sse9I AATT 5 cut(s) 95, 328, 528, 567, 606
SspI AATATT 1 cut(s) 140
TaaI ACNGT 3 cut(s) 506, 557, 652
TaiI ACGT 1 cut(s) 94
TaqI TCGA 1 cut(s) 480
TaqII GACCGA 2 cut(s) 135, 398
TasI AATT 5 cut(s) 95, 328, 528, 567, 606
Tru1I TTAA 3 cut(s) 105, 395, 594
Tru9I TTAA 3 cut(s) 105, 395, 594
TscAI CASTG 3 cut(s) 354, 554, 562
TseFI GTSAC 1 cut(s) 471
TseI GCWGC 4 cut(s) 45, 48, 51, 598
Tsp45I GTSAC 1 cut(s) 471
TspDTI ATGAA 3 cut(s) 156, 254, 619
TspRI CASTG 3 cut(s) 354, 554, 562
VpaK11BI GGWCC 1 cut(s) 410
XagI CCTNNNNNAGG 1 cut(s) 365
XapI RAATTY 2 cut(s) 328, 567
XceI RCATGY 2 cut(s) 85, 242
XmiI GTMKAC 1 cut(s) 356
XmnI GAANNNNTTC 1 cut(s) 567
Zsp2I ATGCAT 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.