RLG00000020279

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0019448
Physical Location & Seq
Forward (+)
63189862 .. 63190331
470 bp
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UTR
Exon/CDS
Intron
RLM00000020279

Sequence Viewer

Length: 357 bp
ATGGATTTTCAGGTGAAGATTCAGAGAACCCATTTGTATGATTCCAATTCCAAATTGGAATTACTGAGGCTGTGGTGTTTTGGGCTCCTGAATCCTTTTTGCCAGCGACCTATAAGTTTAAGGAGGAGTGGTGAGATCCTGAGCGAAGATGGGCTTTTTGGAGAGGACCCAAGTTTAGCCTCAGTCCCACCATCTCTTAATAGAGGCGTACTCGCCCATGGAGGAGACCGACTTGGACCGCTGCAGGGCCCAGGTGGTGGCCAGGAAGGAGATGGGCTAGAACTTGAGGGAGAGACACGAGAGCCTTCGTCGGAGAGCCGAAGCCTGGTCGAGGCTGGAGCCCTAGGAGAAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

119

Amino Acids

12.67

Weight (kDa)

4.74

Isoelectric Point (pI)

56.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019448)

Species Orthologous Gene IDs
rosa_laevigata RLG00000001334 RLG00000002601 RLG00000003708 RLG00000005142 RLG00000020279
rosa_multiflora Rmu_ssc0000473.1_g000025
rosa_roxburghii Rroxscaffold_4G00320620
rosa_samantha Rh4DG215400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 257
AciI CCGC 1 cut(s) 239
AclWI GGATC 1 cut(s) 130
AcoI YGGCCR 1 cut(s) 259
AfaI GTAC 1 cut(s) 210
AfiI CCNNNNNNNGG 4 cut(s) 245, 257, 325, 331
AjnI CCWGG 3 cut(s) 250, 261, 324
Alw26I GTCTC 2 cut(s) 219, 287
AlwI GGATC 1 cut(s) 130
AoxI GGCC 2 cut(s) 247, 259
ApaI GGGCCC 1 cut(s) 251
ApeKI GCWGC 1 cut(s) 241
AspA2I CCTAGG 1 cut(s) 343
AspS9I GGNCC 4 cut(s) 166, 236, 247, 248
AsuHPI GGTGA 2 cut(s) 25, 143
AvaII GGWCC 2 cut(s) 166, 236
AvrII CCTAGG 1 cut(s) 343
BaeGI GKGCMC 1 cut(s) 251
BalI TGGCCA 1 cut(s) 261
BanII GRGCYC 3 cut(s) 87, 251, 343
BauI CACGAG 1 cut(s) 297
BbvI GCAGC 1 cut(s) 228
BccI CCATC 3 cut(s) 143, 199, 266
BciT130I CCWGG 3 cut(s) 252, 263, 326
BcoDI GTCTC 2 cut(s) 219, 287
BfaI CTAG 2 cut(s) 278, 344
BfmI CTRYAG 1 cut(s) 242
BisI GCNGC 1 cut(s) 242
BlnI CCTAGG 1 cut(s) 343
BlsI GCNGC 1 cut(s) 243
Bme1390I CCNGG 3 cut(s) 252, 263, 326
Bme18I GGWCC 2 cut(s) 166, 236
BmgT120I GGNCC 4 cut(s) 166, 236, 247, 248
BmiI GGNNCC 4 cut(s) 86, 168, 249, 340
BmrFI CCNGG 3 cut(s) 252, 263, 326
BplI GAGNNNNNCTC 2 cut(s) 195, 227
BpmI CTGGAG 1 cut(s) 357
Bpu10I CCTNAGC 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 305
BsaI GGTCTC 1 cut(s) 219
BsaJI CCNNGG 3 cut(s) 217, 250, 343
BsaXI ACNNNNNCTCC 2 cut(s) 115, 145
Bsc4I CCNNNNNNNGG 4 cut(s) 245, 257, 325, 331
BseBI CCWGG 3 cut(s) 252, 263, 326
BseDI CCNNGG 3 cut(s) 217, 250, 343
BseLI CCNNNNNNNGG 4 cut(s) 245, 257, 325, 331
BseMII CTCAG 3 cut(s) 56, 131, 195
BseRI GAGGAG 2 cut(s) 139, 237
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 1 cut(s) 228
BshFI GGCC 2 cut(s) 249, 261
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 4 cut(s) 245, 257, 325, 331
BsmAI GTCTC 2 cut(s) 219, 287
BsmFI GGGAC 1 cut(s) 170
BsnI GGCC 2 cut(s) 249, 261
Bso31I GGTCTC 1 cut(s) 219
Bsp120I GGGCCC 1 cut(s) 247
Bsp1286I GDGCHC 3 cut(s) 87, 251, 343
Bsp143I GATC 1 cut(s) 135
Bsp19I CCATGG 1 cut(s) 217
BspACI CCGC 1 cut(s) 239
BspANI GGCC 2 cut(s) 249, 261
BspCNI CTCAG 3 cut(s) 57, 132, 194
BspLI GGNNCC 4 cut(s) 86, 168, 249, 340
BspMAI CTGCAG 1 cut(s) 246
BspPI GGATC 1 cut(s) 130
BspTNI GGTCTC 1 cut(s) 219
BssECI CCNNGG 3 cut(s) 217, 250, 343
BssMI GATC 1 cut(s) 135
BssSI CACGAG 1 cut(s) 297
BssT1I CCWWGG 2 cut(s) 217, 343
Bst2BI CACGAG 1 cut(s) 297
Bst2UI CCWGG 3 cut(s) 252, 263, 326
BstC8I GCNNGC 1 cut(s) 104
BstDEI CTNAG 3 cut(s) 65, 140, 181
BstDSI CCRYGG 1 cut(s) 217
BstENI CCTNNNNNAGG 1 cut(s) 329
BstKTI GATC 1 cut(s) 138
BstMAI GTCTC 2 cut(s) 219, 287
BstMBI GATC 1 cut(s) 135
BstNI CCWGG 3 cut(s) 252, 263, 326
BstSCI CCNGG 3 cut(s) 250, 261, 324
BstSFI CTRYAG 1 cut(s) 242
BstSLI GKGCMC 1 cut(s) 251
BstV1I GCAGC 1 cut(s) 228
BstX2I RGATCY 1 cut(s) 135
BstYI RGATCY 1 cut(s) 135
BsuRI GGCC 2 cut(s) 249, 261
BtgI CCRYGG 1 cut(s) 217
Cac8I GCNNGC 1 cut(s) 104
Cfr13I GGNCC 4 cut(s) 166, 236, 247, 248
Csp6I GTAC 1 cut(s) 209
CviAII CATG 1 cut(s) 218
CviQI GTAC 1 cut(s) 209
DdeI CTNAG 3 cut(s) 65, 140, 181
DpnI GATC 1 cut(s) 137
DpnII GATC 1 cut(s) 135
EaeI YGGCCR 1 cut(s) 259
Eco130I CCWWGG 2 cut(s) 217, 343
Eco24I GRGCYC 3 cut(s) 87, 251, 343
Eco31I GGTCTC 1 cut(s) 219
Eco47I GGWCC 2 cut(s) 166, 236
EcoNI CCTNNNNNAGG 1 cut(s) 329
EcoO109I RGGNCCY 2 cut(s) 166, 247
EcoRII CCWGG 3 cut(s) 250, 261, 324
EcoT14I CCWWGG 2 cut(s) 217, 343
EcoT38I GRGCYC 3 cut(s) 87, 251, 343
ErhI CCWWGG 2 cut(s) 217, 343
FaeI CATG 1 cut(s) 221
FaiI YATR 3 cut(s) 39, 113, 219
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FaqI GGGAC 1 cut(s) 170
FatI CATG 1 cut(s) 217
Fnu4HI GCNGC 1 cut(s) 242
FriOI GRGCYC 3 cut(s) 87, 251, 343
Fsp4HI GCNGC 1 cut(s) 242
FspBI CTAG 2 cut(s) 278, 344
GluI GCNGC 1 cut(s) 242
GsuI CTGGAG 1 cut(s) 357
HaeIII GGCC 2 cut(s) 249, 261
Hin1II CATG 1 cut(s) 221
HinfI GANTC 3 cut(s) 19, 41, 91
HphI GGTGA 2 cut(s) 25, 143
Hpy188I TCNGA 2 cut(s) 24, 313
Hpy188III TCNNGA 2 cut(s) 88, 139
Hpy99I CGWCG 1 cut(s) 313
HpyAV CCTTC 3 cut(s) 260, 315, 344
HpyCH4V TGCA 1 cut(s) 244
HpyF3I CTNAG 3 cut(s) 65, 140, 181
Hsp92II CATG 1 cut(s) 221
Kzo9I GATC 1 cut(s) 135
LmnI GCTCC 2 cut(s) 90, 338
Lsp1109I GCAGC 1 cut(s) 228
MaeI CTAG 2 cut(s) 278, 344
MalI GATC 1 cut(s) 137
MboI GATC 1 cut(s) 135
MboII GAAGA 2 cut(s) 28, 158
MflI RGATCY 1 cut(s) 135
MhlI GDGCHC 3 cut(s) 87, 251, 343
MlsI TGGCCA 1 cut(s) 261
MluCI AATT 3 cut(s) 46, 53, 59
MluNI TGGCCA 1 cut(s) 261
MmeI TCCRAC 1 cut(s) 291
MnlI CCTC 8 cut(s) 60, 117, 157, 190, 197, 215, 280, 325
Mox20I TGGCCA 1 cut(s) 261
MscI TGGCCA 1 cut(s) 261
MseI TTAA 2 cut(s) 119, 198
MslI CAYNNNNRTG 1 cut(s) 36
Msp20I TGGCCA 1 cut(s) 261
MspA1I CMGCKG 1 cut(s) 241
MspR9I CCNGG 3 cut(s) 252, 263, 326
MvaI CCWGG 3 cut(s) 252, 263, 326
NcoI CCATGG 1 cut(s) 217
NdeII GATC 1 cut(s) 135
NlaIII CATG 1 cut(s) 221
NlaIV GGNNCC 4 cut(s) 86, 168, 249, 340
PfeI GAWTC 3 cut(s) 19, 41, 91
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 1 cut(s) 243
PpuMI RGGWCCY 1 cut(s) 166
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 3 cut(s) 250, 261, 324
PspGI CCWGG 3 cut(s) 250, 261, 324
PspN4I GGNNCC 4 cut(s) 86, 168, 249, 340
PspOMI GGGCCC 1 cut(s) 247
PspPI GGNCC 4 cut(s) 166, 236, 247, 248
PspPPI RGGWCCY 1 cut(s) 166
PstI CTGCAG 1 cut(s) 246
PsuI RGATCY 1 cut(s) 135
RsaI GTAC 1 cut(s) 210
RsaNI GTAC 1 cut(s) 209
RseI CAYNNNNRTG 1 cut(s) 36
SaqAI TTAA 2 cut(s) 119, 198
SatI GCNGC 1 cut(s) 242
Sau3AI GATC 1 cut(s) 135
Sau96I GGNCC 4 cut(s) 166, 236, 247, 248
ScrFI CCNGG 3 cut(s) 252, 263, 326
SduI GDGCHC 3 cut(s) 87, 251, 343
SetI ASST 3 cut(s) 15, 112, 256
SfcI CTRYAG 1 cut(s) 242
SinI GGWCC 2 cut(s) 166, 236
SmiMI CAYNNNNRTG 1 cut(s) 36
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 3 cut(s) 46, 53, 59
SsiI CCGC 1 cut(s) 239
SspMI CTAG 2 cut(s) 278, 344
StyD4I CCNGG 3 cut(s) 250, 261, 324
StyI CCWWGG 2 cut(s) 217, 343
TaqI TCGA 1 cut(s) 330
TaqII GACCGA 1 cut(s) 243
TasI AATT 3 cut(s) 46, 53, 59
TfiI GAWTC 3 cut(s) 19, 41, 91
Tru1I TTAA 2 cut(s) 119, 198
Tru9I TTAA 2 cut(s) 119, 198
TseI GCWGC 1 cut(s) 241
Van91I CCANNNNNTGG 1 cut(s) 257
VpaK11BI GGWCC 2 cut(s) 166, 236
XagI CCTNNNNNAGG 1 cut(s) 329
XcmI CCANNNNNNNNNTGG 2 cut(s) 52, 269
XmaJI CCTAGG 1 cut(s) 343
XspI CTAG 2 cut(s) 278, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.