RLG00000020594

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0019071
Physical Location & Seq
Forward (+)
66595920 .. 66597786
1867 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020594

Sequence Viewer

Length: 525 bp
ATGTCAAATTATGTTAAGAGTGAAGACATCAGGGTACGGGGAATTCGATTTGAGTTTACTGAGGGCGCAGCAGAGGCTATGAGCATGAGGATAGACGGAGCACCATGGGGAAACATCCCACTTGATGTCATCACATCGATTGAAATCTCTTATCACGGTAAACTCAACATCCTTGCCCATCCAAATCGTGATTGCCCAGCAAAGAGTATCCACGAGTCGTCACAACCCCGCGTTTCTGAAACCGATGTTAACAACAATATTGCTAAAGATGGCTCCTCTGGAGGACATAAGGAGCTCCTGGGAGTAGCAAGCACTGATTCCAAACTTCCCATTGATGATAACAAGGCTAAAGACCACTCTGAAAGCCATAAGACGTTGGGTAATGTAGCAAGCAGTTCCAAAATCCCCAGTCATGATGGCAATGCTGAAGATTATTACCATGAATGGCGTCATAAGAAGTTTGGTGCAGCAAGTACCTTCAAATACAATCCTGAAATTTGGAGGTTCCGACGTTATGATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

175

Amino Acids

19.56

Weight (kDa)

6.5

Isoelectric Point (pI)

55.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019071)

Species Orthologous Gene IDs
rosa_laevigata RLG00000020594
rosa_roxburghii Rroxscaffold_2G00096100 Rroxscaffold_2G00101130
rosa_rugosa Rorug02G0429800 Rorug02G0429900 Rorug02G0430000
rosa_samantha Rh2AG490500 Rh2CG476900
rosa_wichuraiana Rw0G020230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 231
AciI CCGC 1 cut(s) 229
AcsI RAATTY 2 cut(s) 42, 495
AcuI CTGAAG 1 cut(s) 447
AcyI GRCGYC 1 cut(s) 448
AfaI GTAC 2 cut(s) 36, 475
AgsI TTSAA 2 cut(s) 143, 481
AjnI CCWGG 1 cut(s) 297
AluBI AGCT 1 cut(s) 295
AluI AGCT 1 cut(s) 295
Alw21I GWGCWC 2 cut(s) 103, 297
ApeKI GCWGC 2 cut(s) 68, 467
ApoI RAATTY 2 cut(s) 42, 495
AspLEI GCGC 1 cut(s) 68
BanII GRGCYC 1 cut(s) 297
BauI CACGAG 1 cut(s) 212
BbsI GAAGAC 1 cut(s) 30
Bbv12I GWGCWC 2 cut(s) 103, 297
BbvI GCAGC 2 cut(s) 80, 479
BccI CCATC 3 cut(s) 186, 263, 410
BciT130I CCWGG 1 cut(s) 299
BciVI GTATCC 1 cut(s) 218
BfuI GTATCC 1 cut(s) 218
BisI GCNGC 2 cut(s) 69, 468
BlsI GCNGC 2 cut(s) 70, 469
Bme1390I CCNGG 1 cut(s) 299
BmiI GGNNCC 2 cut(s) 274, 506
BmrFI CCNGG 1 cut(s) 299
BmrI ACTGGG 1 cut(s) 402
BmuI ACTGGG 1 cut(s) 402
BpiI GAAGAC 1 cut(s) 30
BpmI CTGGAG 1 cut(s) 300
Bsa29I ATCGAT 1 cut(s) 137
BsaBI GATNNNNATC 1 cut(s) 143
BsaHI GRCGYC 1 cut(s) 448
BsaJI CCNNGG 2 cut(s) 104, 298
Bse1I ACTGG 1 cut(s) 408
Bse3DI GCAATG 1 cut(s) 427
Bse8I GATNNNNATC 1 cut(s) 143
BseBI CCWGG 1 cut(s) 299
BseCI ATCGAT 1 cut(s) 137
BseDI CCNNGG 2 cut(s) 104, 298
BseGI GGATG 3 cut(s) 114, 168, 178
BseJI GATNNNNATC 1 cut(s) 143
BseMI GCAATG 1 cut(s) 427
BseMII CTCAG 1 cut(s) 51
BseNI ACTGG 1 cut(s) 408
BseRI GAGGAG 1 cut(s) 265
BseXI GCAGC 2 cut(s) 80, 479
BseYI CCCAGC 1 cut(s) 196
BsgI GTGCAG 1 cut(s) 486
Bsh1236I CGCG 1 cut(s) 231
BshVI ATCGAT 1 cut(s) 137
BsiHKAI GWGCWC 2 cut(s) 103, 297
Bsp1286I GDGCHC 2 cut(s) 103, 297
Bsp19I CCATGG 1 cut(s) 104
BspACI CCGC 1 cut(s) 229
BspCNI CTCAG 1 cut(s) 52
BspDI ATCGAT 1 cut(s) 137
BspFNI CGCG 1 cut(s) 231
BspHI TCATGA 1 cut(s) 412
BspLI GGNNCC 2 cut(s) 274, 506
BsrDI GCAATG 1 cut(s) 427
BsrI ACTGG 1 cut(s) 408
BssECI CCNNGG 2 cut(s) 104, 298
BssNI GRCGYC 1 cut(s) 448
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 1 cut(s) 104
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 1 cut(s) 299
Bst4CI ACNGT 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 448
BstC8I GCNNGC 2 cut(s) 310, 391
BstDEI CTNAG 1 cut(s) 60
BstDSI CCRYGG 1 cut(s) 104
BstF5I GGATG 3 cut(s) 114, 168, 178
BstFNI CGCG 1 cut(s) 231
BstHHI GCGC 1 cut(s) 68
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstNI CCWGG 1 cut(s) 299
BstSCI CCNGG 1 cut(s) 297
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 80, 479
BstV2I GAAGAC 1 cut(s) 30
Bsu15I ATCGAT 1 cut(s) 137
BsuI GTATCC 1 cut(s) 218
BsuTUI ATCGAT 1 cut(s) 137
BtgI CCRYGG 1 cut(s) 104
BtsCI GGATG 3 cut(s) 114, 168, 178
BtsIMutI CAGTG 1 cut(s) 312
Cac8I GCNNGC 2 cut(s) 310, 391
CciI TCATGA 1 cut(s) 412
CfoI GCGC 1 cut(s) 68
ClaI ATCGAT 1 cut(s) 137
CseI GACGC 1 cut(s) 437
Csp6I GTAC 2 cut(s) 35, 474
CviAII CATG 4 cut(s) 85, 105, 413, 440
CviJI RGCY 5 cut(s) 77, 273, 295, 347, 366
CviKI_1 RGCY 5 cut(s) 77, 273, 295, 347, 366
CviQI GTAC 2 cut(s) 35, 474
DdeI CTNAG 1 cut(s) 60
Ecl136II GAGCTC 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 104
Eco24I GRGCYC 1 cut(s) 297
Eco53kI GAGCTC 1 cut(s) 295
Eco57I CTGAAG 1 cut(s) 447
EcoICRI GAGCTC 1 cut(s) 295
EcoRI GAATTC 1 cut(s) 42
EcoRII CCWGG 1 cut(s) 297
EcoT14I CCWWGG 1 cut(s) 104
EcoT38I GRGCYC 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 104
FaeI CATG 4 cut(s) 88, 108, 416, 443
FatI CATG 4 cut(s) 84, 104, 412, 439
FauI CCCGC 1 cut(s) 236
Fnu4HI GCNGC 2 cut(s) 69, 468
FokI GGATG 3 cut(s) 101, 155, 165
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 2 cut(s) 69, 468
GlaI GCGC 1 cut(s) 67
GluI GCNGC 2 cut(s) 69, 468
GsaI CCCAGC 1 cut(s) 200
GsuI CTGGAG 1 cut(s) 300
HgaI GACGC 1 cut(s) 437
HhaI GCGC 1 cut(s) 68
Hin1I GRCGYC 1 cut(s) 448
Hin1II CATG 4 cut(s) 88, 108, 416, 443
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HinfI GANTC 2 cut(s) 215, 317
HpaI GTTAAC 1 cut(s) 250
Hpy166II GTNNAC 3 cut(s) 57, 161, 250
Hpy188I TCNGA 3 cut(s) 238, 361, 509
Hpy188III TCNNGA 4 cut(s) 188, 279, 413, 491
Hpy8I GTNNAC 3 cut(s) 57, 161, 250
Hpy99I CGWCG 1 cut(s) 513
HpyAV CCTTC 1 cut(s) 487
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4IV ACGT 2 cut(s) 374, 511
HpyCH4V TGCA 1 cut(s) 467
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpyF3I CTNAG 1 cut(s) 60
HpySE526I ACGT 2 cut(s) 374, 511
Hsp92I GRCGYC 1 cut(s) 448
Hsp92II CATG 4 cut(s) 88, 108, 416, 443
HspAI GCGC 1 cut(s) 66
KspAI GTTAAC 1 cut(s) 250
LmnI GCTCC 4 cut(s) 98, 278, 292, 300
LpnPI CCDG 7 cut(s) 16, 210, 264, 284, 311, 421, 504
Lsp1109I GCAGC 2 cut(s) 80, 479
MaeII ACGT 2 cut(s) 374, 511
MaeIII GTNAC 1 cut(s) 219
MboII GAAGA 2 cut(s) 35, 440
MhlI GDGCHC 2 cut(s) 103, 297
MluCI AATT 3 cut(s) 7, 42, 495
MlyI GAGTC 1 cut(s) 224
MnlI CCTC 6 cut(s) 55, 67, 81, 275, 286, 495
MseI TTAA 2 cut(s) 15, 249
MspR9I CCNGG 1 cut(s) 299
MvaI CCWGG 1 cut(s) 299
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 1 cut(s) 74
NcoI CCATGG 1 cut(s) 104
NlaIII CATG 4 cut(s) 88, 108, 416, 443
NlaIV GGNNCC 2 cut(s) 274, 506
NmuCI GTSAC 1 cut(s) 219
PagI TCATGA 1 cut(s) 412
PcsI WCGNNNNNNNCGW 1 cut(s) 43
PfeI GAWTC 1 cut(s) 317
PkrI GCNGC 2 cut(s) 70, 469
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
Psp124BI GAGCTC 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 297
PspFI CCCAGC 1 cut(s) 196
PspGI CCWGG 1 cut(s) 297
PspN4I GGNNCC 2 cut(s) 274, 506
RsaI GTAC 2 cut(s) 36, 475
RsaNI GTAC 2 cut(s) 35, 474
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 2 cut(s) 15, 249
SatI GCNGC 2 cut(s) 69, 468
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 1 cut(s) 299
SduI GDGCHC 2 cut(s) 103, 297
SetI ASST 5 cut(s) 297, 377, 479, 506, 514
Sse9I AATT 3 cut(s) 7, 42, 495
SsiI CCGC 1 cut(s) 229
SspI AATATT 1 cut(s) 259
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 297
StyI CCWWGG 1 cut(s) 104
TaaI ACNGT 1 cut(s) 158
TaiI ACGT 2 cut(s) 377, 514
TaqI TCGA 2 cut(s) 46, 137
TasI AATT 3 cut(s) 7, 42, 495
TfiI GAWTC 1 cut(s) 317
Tru1I TTAA 2 cut(s) 15, 249
Tru9I TTAA 2 cut(s) 15, 249
TscAI CASTG 1 cut(s) 319
TseFI GTSAC 1 cut(s) 219
TseI GCWGC 2 cut(s) 68, 467
Tsp45I GTSAC 1 cut(s) 219
TspDTI ATGAA 1 cut(s) 456
TspGWI ACGGA 1 cut(s) 111
TspRI CASTG 1 cut(s) 319
XapI RAATTY 2 cut(s) 42, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.