Rorug02G0429800

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0019071
Physical Location & Seq
Reverse (-)
55116244 .. 55119203
2960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0429800.1

Sequence Viewer

Length: 2865 bp
ATGTTCCGGGAAGTTTTGGCCGACTGTAATCTGGAGGATATGGGTTTCGTGGGTTCTCGCTTTACCTGGGCGAACTGTTATACGAAGAAACGTTTGGACCGGAGTTGTTGCACTCATAATTGGCGTGCACTCTATCCGTATTCTAGGGTTGTCACACTGCCTCCTAACAGGTCAGATCATAATCCCATTGTTGTAGAGGTAGCTGCTGCACCGGTTGTCAGAGTGCCAAGGTCTAGGCGTTTTAGGTTCGAGAATATGAGGATGCAACATGAAGAATGTGCTGCTGTTATTCAGAAAGGATGGTCCATTCCCTCTATTGGTGAACCTCTGAAGCAGGTTGGATTGAAAATTGGTTCAACAGGTAAACTTCTGATGGAATGGGATGCGGGAACTTTTCGGCAGAGGAAAACGGAGATGTTTCTTTTGCAATCTAAGATGGATATGTTGATGAAACTTCCGTTTGATCCTTCTCACTTTGAACAACAACAAGCTCTTCAATTTAGGTATAACGAACTTCTATCCTTGGAAGAAGCTTATTGGCGCCAGCGGTCTAGAGTATTATGGTTGAAAGAGGGGGACCGTAATTCAGCTTTTTTCCACAGGAAACCTTCTAACAGACATAGCCGTAATAAGGTGGTCGGTATCACTGATTTGTCAGGGCAATGGCAAACTGATCCTGCTCAGGTAGCCTCAATTTTTGTTGATTATTATGAAAATATTTTTCGCTCAGAGGGTTCTGATTCTGCAGCTTTGGAGGTAGTTCTTGAACAGATTCAACCATGTGTTGATAATGATATGAATTCTTCTCTATTAGCTGCATATTCAGATGCGGAAATTAAGAAGGCTTTGTTTCAGATGCATCCATCAAAATCACCCGGACCTGATGGTATGTCTCCTGTTTTTTTCCAGAAACACTGGGCTATTGTTCAGCATGATGTGTGTTTAGCGGTGAAAACTTTTTTAAACACAGGTATTCTGGATTCTGAGAGCAATTTTACTCATTTGACTCTTATTCCAAAAATTAAAGAGCCTAAGATTGCAGCTGATTTCAGGCCTATTGCTCTATGTAATGTGGTGTATAAAATTGCTTCTAAGGTTCTTGCTAACAGATTGAAAGTCTTACTACCTGGAATCATCTCCCCTCTTCAAAGTGCCTTTGTTCCAGGTCGTTTGATATCTGATAACACTTTGGTTGCCTCTGAGGTTTCTCATTTTATGCATAAGCTTCGGTATCAGAATGAGGGTTTCTTCTCTCTCAAGTTAGATATCAGTAAAGCTTATGATAGATTGGAGTGGACTTTTCTGACTGCTATTTTATCTAAGATGGGTTTTGCGCAGAAGTGGATTGATACTGTTCTATGTTCTATTAGAACAGTGCGCTATTCTATTCTTTTACATGGTACACCTACAGTCTCTCAGGGATCACTGAAAGGGTTGCGTATGAGTCCTGGGGCCCCAGTTGTTCATCATTTGTTATTTACAGATGATAGTTTTCTATTTGGGGAAGCATCTGTTTCAGAATGTGCTACCCTCAAGTCCATCTTGAATGTTTACGAGCGTGCTTCTGGTCAAAAGATTAACTTGGATAAAAGCAGTGTTGTTTTCAGTAAGAATGTGCATCCTGATGTCAAAGGTACTTTAGCTTCTATTTTAGGGGTGCAATGTGTTGAGGATCATGGCAGATATCTAGGGCTACCCCTTCATGTTGGGAAATCAAAAACAGTAATTTTCTCTTACTTAAAGGAAAGACTGACAAAGAAGCTGATCAGCTGGCGCACCAAAATCTTGAGTTCTGCTGGTAAGGAGATTATAATTAAGGTTGTGGCTCAAGTTATTCCAACTTATGTTATGAGTTGTTATATGCTTCCTAAAGGTCTCTGTGATGATTTGCATCAACTTTGTGCTCAATTCTTTTGGGGAGATTCAGATTCTAATCGCAAAATTCACTGGAGGTCTTGGGAAAGGATGTGTCTGTCTAAAAGTGAGGGAGGTCTAGGCTTCAAGAATCTGCATGCCCATAATTTATCTATGCTTGCAAAACAGGGGTGGAGGCTAATCACTAATCCTAACTCATTATTGTCTCAATTGCTTAAGGCTAGATACTTTCCAACTGGTTCTTTTCTAGATGCGGATATGGGGGATTCCCCTTCCTTTGCATGGAGAAGCATTATGGAAGTTAGAACTGTGCTTCAGGCTGGTTTACTTTGGCAGGTTGGCACGGGCACCTCAATCAATGTTTGGAATGATCAGTGGATTCCAAACATTTCTCATCATCACTTGCGTAGACCGTTTGCTAGTGACATTGAGTCTGTCTCTTCTTTGTTTGATGCAGCTACTAGGAGTTGGAGGTTGGATGTTCTGCAACCTGTCTTTTCTCAGGAAGTTGTTGATGCCATTTTGTGTATTCCTCTCAGTAGCAGAGATAGGAGAGATCGCCTGAGTTGGAAGTTTGAGAAGAAAGGCAGGTTTTCTACTAAATCTGCCTATTTTGTAGCTAGGAATATGGTGATGGGGGATTTCTTTGCTTCTTCCTCTCTTGGTGACCCTTTCAGGACTCTTTGGAAGACATTATGGAAAGCTAATATCCCCGGGAAGGTTTCTATTTGTATCTGGAGAGCATGTCACAATGTGCTACCAACAAGAGAGAGTTTAAGCAAGAAGGGCTATACTGGTGATATGGGCTGTTTGCTTTGTTCACACCAGTTAGAGAGTGTTGGACATGTGCTTTGTGGTTGTCCTGCTGCACAGAAAATCCTTGCAGCTCCTCCTTTTTCCATTCAGGTTCCTATTACTCCTTTTATTTTCAAGGAATGGTTACTAGAACAGGCTTCCAGTCTCTCATCAGAAATTTTTCTAAGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

954

Amino Acids

108.06

Weight (kDa)

9.3

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 345 - 565 4.8e-15 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 823 - 917 2.9e-15 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019071)

Species Orthologous Gene IDs
rosa_laevigata RLG00000020594
rosa_roxburghii Rroxscaffold_2G00096100 Rroxscaffold_2G00101130
rosa_rugosa Rorug02G0429800 Rorug02G0429900 Rorug02G0430000
rosa_samantha Rh2AG490500 Rh2CG476900
rosa_wichuraiana Rw0G020230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1811
Acc16I TGCGCA 1 cut(s) 1335
Acc36I ACCTGC 3 cut(s) 325, 2200, 2454
AccB1I GGYRCC 2 cut(s) 540, 2222
AccI GTMKAC 1 cut(s) 2284
AciI CCGC 5 cut(s) 386, 547, 830, 947, 2129
AclI AACGTT 1 cut(s) 91
AclWI GGATC 4 cut(s) 458, 668, 1429, 1680
AcoI YGGCCR 1 cut(s) 18
AcsI RAATTY 3 cut(s) 799, 1941, 2847
AcuI CTGAAG 2 cut(s) 350, 2174
AcyI GRCGYC 1 cut(s) 541
AdeI CACNNNGTG 1 cut(s) 2629
AfaI GTAC 2 cut(s) 1402, 1636
AfiI CCNNNNNNNGG 4 cut(s) 317, 631, 2157, 2593
AflII CTTAAG 1 cut(s) 2090
AflIII ACRYGT 1 cut(s) 2719
AgeI ACCGGT 1 cut(s) 211
AhdI GACNNNNNGTC 1 cut(s) 2305
AjnI CCWGG 4 cut(s) 65, 1126, 1162, 1447
AjuI GAANNNNNNNTTGG 2 cut(s) 77, 109
Alw21I GWGCWC 2 cut(s) 130, 1906
Alw26I GTCTC 6 cut(s) 897, 1417, 1880, 2085, 2317, 2840
Alw44I GTGCAC 1 cut(s) 126
AlwI GGATC 4 cut(s) 458, 668, 1429, 1680
Ama87I CYCGRG 1 cut(s) 2588
AoxI GGCC 3 cut(s) 18, 1052, 1452
ApaI GGGCCC 1 cut(s) 1456
ApaLI GTGCAC 1 cut(s) 126
ApeKI GCWGC 9 cut(s) 203, 206, 281, 746, 815, 1040, 2330, 2741, 2759
ApoI RAATTY 3 cut(s) 799, 1941, 2847
ArsI GACNNNNNNTTYG 2 cut(s) 2709, 2741
AsiGI ACCGGT 1 cut(s) 211
Asp700I GAANNNNTTC 2 cut(s) 771, 2850
AspLEI GCGC 4 cut(s) 543, 1336, 1380, 1776
AspS9I GGNCC 6 cut(s) 97, 303, 577, 878, 1452, 1453
AsuC2I CCSGG 4 cut(s) 8, 876, 2589, 2590
AsuHPI GGTGA 6 cut(s) 332, 864, 961, 2518, 2552, 2684
AvaI CYCGRG 1 cut(s) 2588
AvaII GGWCC 4 cut(s) 97, 303, 577, 878
BaeGI GKGCMC 3 cut(s) 130, 1456, 2225
BanI GGYRCC 2 cut(s) 540, 2222
BanII GRGCYC 1 cut(s) 1456
BarI GAAGNNNNNNTAC 2 cut(s) 1627, 1659
BbsI GAAGAC 1 cut(s) 2570
Bbv12I GWGCWC 2 cut(s) 130, 1906
BbvI GCAGC 9 cut(s) 190, 193, 268, 758, 802, 1052, 2342, 2728, 2771
BccI CCATC 8 cut(s) 294, 367, 430, 871, 878, 1318, 1547, 2503
BceAI ACGGC 1 cut(s) 609
BcgI CGANNNNNNTGC 2 cut(s) 1208, 1242
BciT130I CCWGG 4 cut(s) 67, 1128, 1164, 1449
BclI TGATCA 2 cut(s) 1764, 2245
BcnI CCSGG 4 cut(s) 8, 876, 2589, 2590
BcoDI GTCTC 6 cut(s) 897, 1417, 1880, 2085, 2317, 2840
BfmI CTRYAG 2 cut(s) 744, 1407
BfoI RGCGCY 1 cut(s) 544
BfrI CTTAAG 1 cut(s) 2090
BfuAI ACCTGC 3 cut(s) 325, 2200, 2454
BisI GCNGC 9 cut(s) 204, 207, 282, 747, 816, 1041, 2331, 2742, 2760
BlsI GCNGC 9 cut(s) 205, 208, 283, 748, 817, 1042, 2332, 2743, 2761
Bme1390I CCNGG 8 cut(s) 8, 67, 876, 1128, 1164, 1449, 2589, 2590
Bme18I GGWCC 4 cut(s) 97, 303, 577, 878
BmeRI GACNNNNNGTC 1 cut(s) 2305
BmeT110I CYCGRG 1 cut(s) 2588
BmgT120I GGNCC 6 cut(s) 97, 303, 577, 878, 1452, 1453
BmiI GGNNCC 7 cut(s) 542, 578, 1453, 1454, 1455, 2224, 2784
BmrFI CCNGG 8 cut(s) 8, 67, 876, 1128, 1164, 1449, 2589, 2590
BmrI ACTGGG 2 cut(s) 925, 1451
BmuI ACTGGG 2 cut(s) 925, 1451
BpiI GAAGAC 1 cut(s) 2570
BplI GAGNNNNNCTC 2 cut(s) 2297, 2329
BpmI CTGGAG 3 cut(s) 53, 1969, 2632
Bpu10I CCTNAGC 1 cut(s) 681
BpuEI CTTGAG 4 cut(s) 1241, 1517, 1807, 1812
BpuMI CCSGG 4 cut(s) 8, 876, 2589, 2590
BsaBI GATNNNNATC 3 cut(s) 180, 1890, 1932
BsaHI GRCGYC 1 cut(s) 541
BsaI GGTCTC 1 cut(s) 1880
BsaJI CCNNGG 6 cut(s) 66, 227, 522, 1448, 2587, 2588
BsaWI WCCGGW 2 cut(s) 99, 211
BsaXI ACNNNNNCTCC 4 cut(s) 145, 175, 2605, 2635
Bsc4I CCNNNNNNNGG 4 cut(s) 317, 631, 2157, 2593
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 7 cut(s) 920, 1457, 1952, 2116, 2674, 2701, 2832
Bse3DI GCAATG 2 cut(s) 668, 1667
Bse8I GATNNNNATC 3 cut(s) 180, 1890, 1932
BseBI CCWGG 4 cut(s) 67, 1128, 1164, 1449
BseDI CCNNGG 6 cut(s) 66, 227, 522, 1448, 2587, 2588
BseGI GGATG 7 cut(s) 267, 305, 388, 859, 1618, 1971, 2359
BseJI GATNNNNATC 3 cut(s) 180, 1890, 1932
BseLI CCNNNNNNNGG 4 cut(s) 317, 631, 2157, 2593
BseMI GCAATG 2 cut(s) 668, 1667
BseMII CTCAG 8 cut(s) 695, 741, 975, 1191, 1430, 2390, 2425, 2429
BseNI ACTGG 7 cut(s) 920, 1457, 1952, 2116, 2674, 2701, 2832
BseRI GAGGAG 1 cut(s) 2754
BseSI GKGCMC 3 cut(s) 130, 1456, 2225
BseXI GCAGC 9 cut(s) 190, 193, 268, 758, 802, 1052, 2342, 2728, 2771
BsgI GTGCAG 2 cut(s) 192, 2727
BshFI GGCC 3 cut(s) 20, 1054, 1454
BshNI GGYRCC 2 cut(s) 540, 2222
BshTI ACCGGT 1 cut(s) 211
BsiHKAI GWGCWC 2 cut(s) 130, 1906
BsiHKCI CYCGRG 1 cut(s) 2588
BsiSI CCGG 5 cut(s) 7, 100, 212, 876, 2589
BslFI GGGAC 1 cut(s) 590
BslI CCNNNNNNNGG 4 cut(s) 317, 631, 2157, 2593
BsmAI GTCTC 6 cut(s) 897, 1417, 1880, 2085, 2317, 2840
BsmFI GGGAC 1 cut(s) 590
BsnI GGCC 3 cut(s) 20, 1054, 1454
Bso31I GGTCTC 1 cut(s) 1880
BsoBI CYCGRG 1 cut(s) 2588
Bsp120I GGGCCC 1 cut(s) 1452
Bsp1286I GDGCHC 4 cut(s) 130, 1456, 1906, 2225
Bsp143I GATC 8 cut(s) 175, 463, 673, 1421, 1672, 1764, 2245, 2431
BspACI CCGC 5 cut(s) 386, 547, 830, 947, 2129
BspANI GGCC 3 cut(s) 20, 1054, 1454
BspCNI CTCAG 8 cut(s) 694, 740, 976, 1192, 1429, 2389, 2424, 2430
BspLI GGNNCC 7 cut(s) 542, 578, 1453, 1454, 1455, 2224, 2784
BspMAI CTGCAG 1 cut(s) 748
BspMI ACCTGC 3 cut(s) 325, 2200, 2454
BspPI GGATC 4 cut(s) 458, 668, 1429, 1680
BspQI GCTCTTC 1 cut(s) 498
BspT107I GGYRCC 2 cut(s) 540, 2222
BspTI CTTAAG 1 cut(s) 2090
BspTNI GGTCTC 1 cut(s) 1880
BsrDI GCAATG 2 cut(s) 668, 1667
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 7 cut(s) 920, 1457, 1952, 2116, 2674, 2701, 2832
BssAI RCCGGY 1 cut(s) 211
BssECI CCNNGG 6 cut(s) 66, 227, 522, 1448, 2587, 2588
BssMI GATC 8 cut(s) 175, 463, 673, 1421, 1672, 1764, 2245, 2431
BssNI GRCGYC 1 cut(s) 541
BssT1I CCWWGG 2 cut(s) 227, 522
Bst2UI CCWGG 4 cut(s) 67, 1128, 1164, 1449
Bst4CI ACNGT 9 cut(s) 26, 77, 581, 1354, 1375, 1411, 1723, 2185, 2289
Bst6I CTCTTC 3 cut(s) 498, 1149, 2320
BstACI GRCGYC 1 cut(s) 541
BstAFI CTTAAG 1 cut(s) 2090
BstC8I GCNNGC 6 cut(s) 126, 545, 1560, 1772, 2013, 2034
BstEII GGTNACC 1 cut(s) 2540
BstF5I GGATG 7 cut(s) 267, 305, 388, 859, 1618, 1971, 2359
BstH2I RGCGCY 1 cut(s) 544
BstHHI GCGC 4 cut(s) 543, 1336, 1380, 1776
BstKTI GATC 8 cut(s) 178, 466, 676, 1424, 1675, 1767, 2248, 2434
BstMAI GTCTC 6 cut(s) 897, 1417, 1880, 2085, 2317, 2840
BstMBI GATC 8 cut(s) 175, 463, 673, 1421, 1672, 1764, 2245, 2431
BstMWI GCNNNNNNNGC 2 cut(s) 686, 2661
BstNI CCWGG 4 cut(s) 67, 1128, 1164, 1449
BstNSI RCATGY 3 cut(s) 2015, 2622, 2723
BstPI GGTNACC 1 cut(s) 2540
BstSCI CCNGG 8 cut(s) 6, 65, 874, 1126, 1162, 1447, 2587, 2588
BstSFI CTRYAG 2 cut(s) 744, 1407
BstSLI GKGCMC 3 cut(s) 130, 1456, 2225
BstV1I GCAGC 9 cut(s) 190, 193, 268, 758, 802, 1052, 2342, 2728, 2771
BstV2I GAAGAC 1 cut(s) 2570
BsuRI GGCC 3 cut(s) 20, 1054, 1454
BtsCI GGATG 7 cut(s) 267, 305, 388, 859, 1618, 1971, 2359
BtsI GCAGTG 2 cut(s) 155, 1600
BtsIMutI CAGTG 8 cut(s) 155, 645, 913, 1380, 1424, 1600, 1945, 2255
BveI ACCTGC 3 cut(s) 325, 2200, 2454
Cac8I GCNNGC 6 cut(s) 126, 545, 1560, 1772, 2013, 2034
CfoI GCGC 4 cut(s) 543, 1336, 1380, 1776
Cfr10I RCCGGY 1 cut(s) 211
Cfr13I GGNCC 6 cut(s) 97, 303, 577, 878, 1452, 1453
Cfr9I CCCGGG 1 cut(s) 2588
Csp6I GTAC 2 cut(s) 1401, 1635
CspAI ACCGGT 1 cut(s) 211
CviQI GTAC 2 cut(s) 1401, 1635
DinI GGCGCC 1 cut(s) 542
DpnI GATC 8 cut(s) 177, 465, 675, 1423, 1674, 1766, 2247, 2433
DpnII GATC 8 cut(s) 175, 463, 673, 1421, 1672, 1764, 2245, 2431
DraI TTTAAA 1 cut(s) 963
DraIII CACNNNGTG 1 cut(s) 2629
DriI GACNNNNNGTC 1 cut(s) 2305
EaeI YGGCCR 1 cut(s) 18
Eam1104I CTCTTC 3 cut(s) 498, 1149, 2320
Eam1105I GACNNNNNGTC 1 cut(s) 2305
EarI CTCTTC 3 cut(s) 498, 1149, 2320
Eco130I CCWWGG 2 cut(s) 227, 522
Eco147I AGGCCT 1 cut(s) 1054
Eco24I GRGCYC 1 cut(s) 1456
Eco31I GGTCTC 1 cut(s) 1880
Eco32I GATATC 3 cut(s) 1176, 1267, 1685
Eco47I GGWCC 4 cut(s) 97, 303, 577, 878
Eco57I CTGAAG 2 cut(s) 350, 2174
Eco88I CYCGRG 1 cut(s) 2588
Eco91I GGTNACC 1 cut(s) 2540
EcoO109I RGGNCCY 2 cut(s) 1452, 1453
EcoO65I GGTNACC 1 cut(s) 2540
EcoRI GAATTC 1 cut(s) 799
EcoRII CCWGG 4 cut(s) 65, 1126, 1162, 1447
EcoRV GATATC 3 cut(s) 1176, 1267, 1685
EcoT14I CCWWGG 2 cut(s) 227, 522
EcoT22I ATGCAT 2 cut(s) 861, 1221
EcoT38I GRGCYC 1 cut(s) 1456
EgeI GGCGCC 1 cut(s) 542
EheI GGCGCC 1 cut(s) 542
ErhI CCWWGG 2 cut(s) 227, 522
FalI AAGNNNNNCTT 4 cut(s) 1526, 1558, 1565, 1597
FaqI GGGAC 1 cut(s) 590
FauI CCCGC 1 cut(s) 379
FbaI TGATCA 2 cut(s) 1764, 2245
FblI GTMKAC 1 cut(s) 2284
Fnu4HI GCNGC 9 cut(s) 204, 207, 282, 747, 816, 1041, 2331, 2742, 2760
FokI GGATG 7 cut(s) 274, 312, 395, 846, 1605, 1978, 2366
FriOI GRGCYC 1 cut(s) 1456
Fsp4HI GCNGC 9 cut(s) 204, 207, 282, 747, 816, 1041, 2331, 2742, 2760
FspI TGCGCA 1 cut(s) 1335
GlaI GCGC 4 cut(s) 542, 1335, 1379, 1775
GluI GCNGC 9 cut(s) 204, 207, 282, 747, 816, 1041, 2331, 2742, 2760
GsuI CTGGAG 3 cut(s) 53, 1969, 2632
HaeII RGCGCY 1 cut(s) 544
HaeIII GGCC 3 cut(s) 20, 1054, 1454
HapII CCGG 5 cut(s) 7, 100, 212, 876, 2589
HhaI GCGC 4 cut(s) 543, 1336, 1380, 1776
Hin1I GRCGYC 1 cut(s) 541
Hin6I GCGC 4 cut(s) 541, 1334, 1378, 1774
HinP1I GCGC 4 cut(s) 541, 1334, 1378, 1774
HindIII AAGCTT 3 cut(s) 531, 1223, 1275
HpaII CCGG 5 cut(s) 7, 100, 212, 876, 2589
HphI GGTGA 6 cut(s) 332, 864, 961, 2518, 2552, 2684
Hpy166II GTNNAC 9 cut(s) 128, 323, 365, 1296, 1403, 1552, 2201, 2285, 2696
Hpy8I GTNNAC 9 cut(s) 128, 323, 365, 1296, 1403, 1552, 2201, 2285, 2696
HpyAV CCTTC 7 cut(s) 477, 618, 835, 1709, 2157, 2587, 2653
HpyCH4III ACNGT 9 cut(s) 26, 77, 581, 1354, 1375, 1411, 1723, 2185, 2289
HpyCH4IV ACGT 1 cut(s) 91
HpyF10VI GCNNNNNNNGC 2 cut(s) 686, 2661
HpySE526I ACGT 1 cut(s) 91
Hsp92I GRCGYC 1 cut(s) 541
HspAI GCGC 4 cut(s) 541, 1334, 1378, 1774
KasI GGCGCC 1 cut(s) 540
Ksp22I TGATCA 2 cut(s) 1764, 2245
Kzo9I GATC 8 cut(s) 175, 463, 673, 1421, 1672, 1764, 2245, 2431
LguI GCTCTTC 1 cut(s) 498
LmnI GCTCC 1 cut(s) 2767
Lsp1109I GCAGC 9 cut(s) 190, 193, 268, 758, 802, 1052, 2342, 2728, 2771
MaeII ACGT 1 cut(s) 91
MaeIII GTNAC 6 cut(s) 151, 2297, 2540, 2621, 2814, 2858
MalI GATC 8 cut(s) 177, 465, 675, 1423, 1674, 1766, 2247, 2433
MboI GATC 8 cut(s) 175, 463, 673, 1421, 1672, 1764, 2245, 2431
MfeI CAATTG 1 cut(s) 2084
MhlI GDGCHC 4 cut(s) 130, 1456, 1906, 2225
Mly113I GGCGCC 1 cut(s) 541
MlyI GAGTC 4 cut(s) 1000, 1453, 2315, 2548
MmeI TCCRAC 7 cut(s) 319, 1862, 2132, 2324, 2331, 2423, 2695
Mph1103I ATGCAT 2 cut(s) 861, 1221
MroXI GAANNNNTTC 2 cut(s) 771, 2850
MseI TTAA 8 cut(s) 837, 962, 1023, 1578, 1739, 1815, 2091, 2651
MslI CAYNNNNRTG 1 cut(s) 1623
MspA1I CMGCKG 3 cut(s) 547, 1043, 1770
MspCI CTTAAG 1 cut(s) 2090
MspI CCGG 5 cut(s) 7, 100, 212, 876, 2589
MspR9I CCNGG 8 cut(s) 8, 67, 876, 1128, 1164, 1449, 2589, 2590
MunI CAATTG 1 cut(s) 2084
MvaI CCWGG 4 cut(s) 67, 1128, 1164, 1449
MwoI GCNNNNNNNGC 2 cut(s) 686, 2661
NarI GGCGCC 1 cut(s) 541
NciI CCSGG 4 cut(s) 8, 876, 2589, 2590
NdeII GATC 8 cut(s) 175, 463, 673, 1421, 1672, 1764, 2245, 2431
NlaIV GGNNCC 7 cut(s) 542, 578, 1453, 1454, 1455, 2224, 2784
NmuCI GTSAC 4 cut(s) 151, 2297, 2540, 2621
NsbI TGCGCA 1 cut(s) 1335
NsiI ATGCAT 2 cut(s) 861, 1221
NspI RCATGY 3 cut(s) 2015, 2622, 2723
PaeI GCATGC 1 cut(s) 2015
PceI AGGCCT 1 cut(s) 1054
PciI ACATGT 1 cut(s) 2719
PciSI GCTCTTC 1 cut(s) 498
PdmI GAANNNNTTC 2 cut(s) 771, 2850
PfeI GAWTC 9 cut(s) 740, 772, 980, 1131, 1922, 1928, 2005, 2141, 2254
PfoI TCCNGGA 1 cut(s) 6
PinAI ACCGGT 1 cut(s) 211
PkrI GCNGC 9 cut(s) 205, 208, 283, 748, 817, 1042, 2332, 2743, 2761
PleI GAGTC 4 cut(s) 1000, 1452, 2314, 2548
PluTI GGCGCC 1 cut(s) 544
PpsI GAGTC 4 cut(s) 1000, 1452, 2314, 2548
PscI ACATGT 1 cut(s) 2719
PsiI TTATAA 1 cut(s) 1811
Psp1406I AACGTT 1 cut(s) 91
Psp6I CCWGG 4 cut(s) 65, 1126, 1162, 1447
PspEI GGTNACC 1 cut(s) 2540
PspGI CCWGG 4 cut(s) 65, 1126, 1162, 1447
PspN4I GGNNCC 7 cut(s) 542, 578, 1453, 1454, 1455, 2224, 2784
PspOMI GGGCCC 1 cut(s) 1452
PspPI GGNCC 6 cut(s) 97, 303, 577, 878, 1452, 1453
PstI CTGCAG 1 cut(s) 748
PvuII CAGCTG 2 cut(s) 1043, 1770
RsaI GTAC 2 cut(s) 1402, 1636
RsaNI GTAC 2 cut(s) 1401, 1635
RseI CAYNNNNRTG 1 cut(s) 1623
SapI GCTCTTC 1 cut(s) 498
SaqAI TTAA 8 cut(s) 837, 962, 1023, 1578, 1739, 1815, 2091, 2651
SatI GCNGC 9 cut(s) 204, 207, 282, 747, 816, 1041, 2331, 2742, 2760
Sau3AI GATC 8 cut(s) 175, 463, 673, 1421, 1672, 1764, 2245, 2431
Sau96I GGNCC 6 cut(s) 97, 303, 577, 878, 1452, 1453
SchI GAGTC 4 cut(s) 1000, 1453, 2315, 2548
ScrFI CCNGG 8 cut(s) 8, 67, 876, 1128, 1164, 1449, 2589, 2590
SduI GDGCHC 4 cut(s) 130, 1456, 1906, 2225
SfcI CTRYAG 2 cut(s) 744, 1407
SfoI GGCGCC 1 cut(s) 542
SinI GGWCC 4 cut(s) 97, 303, 577, 878
SmaI CCCGGG 1 cut(s) 2590
SmiMI CAYNNNNRTG 1 cut(s) 1623
SmlI CTYRAG 5 cut(s) 1256, 1532, 1786, 1827, 2090
SmoI CTYRAG 5 cut(s) 1256, 1532, 1786, 1827, 2090
SphI GCATGC 1 cut(s) 2015
SseBI AGGCCT 1 cut(s) 1054
SsiI CCGC 5 cut(s) 386, 547, 830, 947, 2129
SspDI GGCGCC 1 cut(s) 540
SspI AATATT 1 cut(s) 718
StuI AGGCCT 1 cut(s) 1054
StyD4I CCNGG 8 cut(s) 6, 65, 874, 1126, 1162, 1447, 2587, 2588
StyI CCWWGG 2 cut(s) 227, 522
TaaI ACNGT 9 cut(s) 26, 77, 581, 1354, 1375, 1411, 1723, 2185, 2289
TaiI ACGT 1 cut(s) 94
TaqI TCGA 1 cut(s) 249
TfiI GAWTC 9 cut(s) 740, 772, 980, 1131, 1922, 1928, 2005, 2141, 2254
Tru1I TTAA 8 cut(s) 837, 962, 1023, 1578, 1739, 1815, 2091, 2651
Tru9I TTAA 8 cut(s) 837, 962, 1023, 1578, 1739, 1815, 2091, 2651
TscAI CASTG 8 cut(s) 162, 652, 920, 1380, 1431, 1600, 1952, 2255
TseFI GTSAC 4 cut(s) 151, 2297, 2540, 2621
TseI GCWGC 9 cut(s) 203, 206, 281, 746, 815, 1040, 2330, 2741, 2759
Tsp45I GTSAC 4 cut(s) 151, 2297, 2540, 2621
TspDTI ATGAA 6 cut(s) 285, 464, 726, 812, 1454, 1691
TspGWI ACGGA 3 cut(s) 126, 425, 447
TspMI CCCGGG 1 cut(s) 2588
TspRI CASTG 8 cut(s) 162, 652, 920, 1380, 1431, 1600, 1952, 2255
Vha464I CTTAAG 1 cut(s) 2090
VneI GTGCAC 1 cut(s) 126
VpaK11BI GGWCC 4 cut(s) 97, 303, 577, 878
XapI RAATTY 3 cut(s) 799, 1941, 2847
XbaI TCTAGA 2 cut(s) 551, 2122
XceI RCATGY 3 cut(s) 2015, 2622, 2723
XmaI CCCGGG 1 cut(s) 2588
XmiI GTMKAC 1 cut(s) 2284
XmnI GAANNNNTTC 2 cut(s) 771, 2850
Zsp2I ATGCAT 2 cut(s) 861, 1221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.