Rorug02G0429900

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0019071
Physical Location & Seq
Forward (+)
55120415 .. 55121200
786 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0429900.1

Sequence Viewer

Length: 786 bp
ATGGCCGTGGCCACTGCTAGCCTTGTGGCTAATTGTTTCTTCCTTCTTATTGCGTTCGTCTGGTTGTTGGGAGCGCCGCCTTGGATTTGGCTCCCGGAGGTGGTCTGGGATGGCAGGTCTGCTATTCTGTTGGTTTCTATGGGGCCGTGCTCTGACAGTGTGGCTTTGAGTTGCTCTATATCGGATCGAAATGCCTGGGTCACGGACCGGACTTGTCCGGTTGGTTTCTCAGCGATGGCTGGGTGTATCAGAGATGGTTGGTTTCTCAGAGATAGCAACGTCGGCACTGGTGGCTCGCTGCAGCGTCGAAGTGTTGCCGGAGTTGGGTTGATCCGACCAGATCAGGGGTCCGATCAAAGCTTGGGTGAGTTTCAGGTTGTGCTCGGCGGCTCTGATCGGCTTCGTCCAGATTTAGGAGCTGGTTTTTTCATTGCTTGTGAAGGTACCGGAATTGATCTGAGATTGATTCGAGGTGATCCGATTTTGGGGCTGACTTGGGTGCCTTTCAACCCTGGAGATGGTGCAGTCTCGTCGGCTGCGTCCCACGGTGACGGCGGCGGCTGGTTGGGCTGTGGAGGAATATGTGGGGTGGTCTTAGCTATTTTGCCCTACTGGGCCAGTTGGGCTTGGAATGGGCTAGAGGGCCTGACGCATGTATGGAGGTGGATGGTTTGGGTTCTACGACTTTTTAGGGCGTGGCTTGTCTTTGGAATGGGGGTTGAGGGCATTTGTTCCCTTTCCGCTCCACCTATGAATTGGGTTGCTATGGCCCATAGTATTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

261

Amino Acids

27.82

Weight (kDa)

5.26

Isoelectric Point (pI)

35.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019071)

Species Orthologous Gene IDs
rosa_laevigata RLG00000020594
rosa_roxburghii Rroxscaffold_2G00096100 Rroxscaffold_2G00101130
rosa_rugosa Rorug02G0429800 Rorug02G0429900 Rorug02G0430000
rosa_samantha Rh2AG490500 Rh2CG476900
rosa_wichuraiana Rw0G020230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 105
Acc65I GGTACC 1 cut(s) 443
AccB1I GGYRCC 2 cut(s) 443, 499
AccBSI CCGCTC 1 cut(s) 743
AciI CCGC 5 cut(s) 77, 387, 555, 558, 741
AclWI GGATC 3 cut(s) 192, 325, 470
AcoI YGGCCR 2 cut(s) 3, 9
AfaI GTAC 1 cut(s) 445
AfiI CCNNNNNNNGG 6 cut(s) 100, 324, 344, 413, 485, 518
AgsI TTSAA 1 cut(s) 508
AjnI CCWGG 2 cut(s) 194, 511
AluBI AGCT 3 cut(s) 360, 419, 599
AluI AGCT 3 cut(s) 360, 419, 599
Alw21I GWGCWC 2 cut(s) 152, 384
Alw26I GTCTC 1 cut(s) 532
AlwI GGATC 3 cut(s) 192, 325, 470
AoxI GGCC 6 cut(s) 3, 9, 143, 615, 643, 768
ApeKI GCWGC 3 cut(s) 298, 301, 536
Asp718I GGTACC 1 cut(s) 443
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 6 cut(s) 143, 205, 348, 615, 643, 769
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 3 cut(s) 377, 485, 560
AsuNHI GCTAGC 1 cut(s) 17
AvaII GGWCC 2 cut(s) 205, 348
BalI TGGCCA 1 cut(s) 11
BanI GGYRCC 2 cut(s) 443, 499
Bbv12I GWGCWC 2 cut(s) 152, 384
BbvI GCAGC 3 cut(s) 285, 313, 523
BccI CCATC 5 cut(s) 104, 229, 248, 512, 661
BceAI ACGGC 2 cut(s) 130, 568
BcgI CGANNNNNNTGC 2 cut(s) 513, 547
BciT130I CCWGG 2 cut(s) 196, 513
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 1 cut(s) 532
BfaI CTAG 2 cut(s) 18, 638
BfmI CTRYAG 1 cut(s) 299
BfoI RGCGCY 1 cut(s) 77
BfuAI ACCTGC 1 cut(s) 105
BglI GCCNNNNNGGC 1 cut(s) 623
BisI GCNGC 7 cut(s) 77, 299, 302, 388, 537, 556, 559
BlsI GCNGC 7 cut(s) 78, 300, 303, 389, 538, 557, 560
Bme1390I CCNGG 3 cut(s) 95, 196, 513
Bme18I GGWCC 2 cut(s) 205, 348
BmgT120I GGNCC 6 cut(s) 143, 205, 348, 615, 643, 769
BmiI GGNNCC 5 cut(s) 92, 144, 349, 445, 501
BmrFI CCNGG 3 cut(s) 95, 196, 513
BmrI ACTGGG 1 cut(s) 622
BmtI GCTAGC 1 cut(s) 21
BmuI ACTGGG 1 cut(s) 622
BpmI CTGGAG 1 cut(s) 534
BpuMI CCSGG 1 cut(s) 95
BsaJI CCNNGG 5 cut(s) 6, 80, 195, 511, 544
BsaWI WCCGGW 3 cut(s) 207, 217, 446
Bsc4I CCNNNNNNNGG 6 cut(s) 100, 324, 344, 413, 485, 518
Bse1I ACTGG 3 cut(s) 292, 617, 618
Bse3DI GCAATG 1 cut(s) 429
BseBI CCWGG 2 cut(s) 196, 513
BseDI CCNNGG 5 cut(s) 6, 80, 195, 511, 544
BseGI GGATG 2 cut(s) 115, 672
BseLI CCNNNNNNNGG 6 cut(s) 100, 324, 344, 413, 485, 518
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 3 cut(s) 243, 280, 449
BseNI ACTGG 3 cut(s) 292, 617, 618
BseXI GCAGC 3 cut(s) 285, 313, 523
BseYI CCCAGC 1 cut(s) 239
BsgI GTGCAG 1 cut(s) 543
BshFI GGCC 6 cut(s) 5, 11, 145, 617, 645, 770
BshNI GGYRCC 2 cut(s) 443, 499
BsiHKAI GWGCWC 2 cut(s) 152, 384
BsiSI CCGG 5 cut(s) 95, 208, 218, 318, 447
BslFI GGGAC 1 cut(s) 526
BslI CCNNNNNNNGG 6 cut(s) 100, 324, 344, 413, 485, 518
BsmAI GTCTC 1 cut(s) 532
BsmFI GGGAC 1 cut(s) 526
BsnI GGCC 6 cut(s) 5, 11, 145, 617, 645, 770
Bsp1286I GDGCHC 2 cut(s) 152, 384
Bsp143I GATC 7 cut(s) 184, 330, 340, 352, 394, 454, 475
BspACI CCGC 5 cut(s) 77, 387, 555, 558, 741
BspANI GGCC 6 cut(s) 5, 11, 145, 617, 645, 770
BspCNI CTCAG 3 cut(s) 242, 279, 450
BspLI GGNNCC 5 cut(s) 92, 144, 349, 445, 501
BspMAI CTGCAG 1 cut(s) 303
BspMI ACCTGC 1 cut(s) 105
BspOI GCTAGC 1 cut(s) 21
BspPI GGATC 3 cut(s) 192, 325, 470
BspT107I GGYRCC 2 cut(s) 443, 499
BsrBI CCGCTC 1 cut(s) 743
BsrDI GCAATG 1 cut(s) 429
BsrI ACTGG 3 cut(s) 292, 617, 618
BssECI CCNNGG 5 cut(s) 6, 80, 195, 511, 544
BssMI GATC 7 cut(s) 184, 330, 340, 352, 394, 454, 475
BssT1I CCWWGG 1 cut(s) 80
Bst2UI CCWGG 2 cut(s) 196, 513
Bst4CI ACNGT 2 cut(s) 158, 548
BstC8I GCNNGC 2 cut(s) 19, 296
BstDEI CTNAG 4 cut(s) 229, 266, 458, 595
BstDSI CCRYGG 2 cut(s) 6, 544
BstF5I GGATG 2 cut(s) 115, 672
BstH2I RGCGCY 1 cut(s) 77
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 7 cut(s) 187, 333, 343, 355, 397, 457, 478
BstMAI GTCTC 1 cut(s) 532
BstMBI GATC 7 cut(s) 184, 330, 340, 352, 394, 454, 475
BstMWI GCNNNNNNNGC 4 cut(s) 282, 291, 567, 623
BstNI CCWGG 2 cut(s) 196, 513
BstNSI RCATGY 1 cut(s) 656
BstSCI CCNGG 3 cut(s) 93, 194, 511
BstSFI CTRYAG 1 cut(s) 299
BstV1I GCAGC 3 cut(s) 285, 313, 523
BsuRI GGCC 6 cut(s) 5, 11, 145, 617, 645, 770
BtgI CCRYGG 2 cut(s) 6, 544
BtgZI GCGATG 1 cut(s) 248
BtsCI GGATG 2 cut(s) 115, 672
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 3 cut(s) 12, 163, 285
BveI ACCTGC 1 cut(s) 105
Cac8I GCNNGC 2 cut(s) 19, 296
CfoI GCGC 1 cut(s) 76
Cfr13I GGNCC 6 cut(s) 143, 205, 348, 615, 643, 769
CpoI CGGWCCG 1 cut(s) 205
CseI GACGC 3 cut(s) 293, 528, 658
Csp6I GTAC 1 cut(s) 444
CspI CGGWCCG 1 cut(s) 205
CviAII CATG 1 cut(s) 653
CviQI GTAC 1 cut(s) 444
DdeI CTNAG 4 cut(s) 229, 266, 458, 595
DpnI GATC 7 cut(s) 186, 332, 342, 354, 396, 456, 477
DpnII GATC 7 cut(s) 184, 330, 340, 352, 394, 454, 475
EaeI YGGCCR 2 cut(s) 3, 9
Eco130I CCWWGG 1 cut(s) 80
Eco47I GGWCC 2 cut(s) 205, 348
EcoO109I RGGNCCY 1 cut(s) 643
EcoRII CCWGG 2 cut(s) 194, 511
EcoT14I CCWWGG 1 cut(s) 80
ErhI CCWWGG 1 cut(s) 80
FaeI CATG 1 cut(s) 656
FaiI YATR 8 cut(s) 140, 179, 583, 654, 658, 752, 767, 774
FaqI GGGAC 1 cut(s) 526
FatI CATG 1 cut(s) 652
Fnu4HI GCNGC 7 cut(s) 77, 299, 302, 388, 537, 556, 559
FokI GGATG 2 cut(s) 122, 679
Fsp4HI GCNGC 7 cut(s) 77, 299, 302, 388, 537, 556, 559
FspBI CTAG 2 cut(s) 18, 638
GlaI GCGC 1 cut(s) 75
GluI GCNGC 7 cut(s) 77, 299, 302, 388, 537, 556, 559
GsaI CCCAGC 1 cut(s) 243
GsuI CTGGAG 1 cut(s) 534
HaeII RGCGCY 1 cut(s) 77
HaeIII GGCC 6 cut(s) 5, 11, 145, 617, 645, 770
HapII CCGG 5 cut(s) 95, 208, 218, 318, 447
HgaI GACGC 3 cut(s) 293, 528, 658
HhaI GCGC 1 cut(s) 76
Hin1II CATG 1 cut(s) 656
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HindIII AAGCTT 1 cut(s) 358
HinfI GANTC 1 cut(s) 466
HpaII CCGG 5 cut(s) 95, 208, 218, 318, 447
HphI GGTGA 3 cut(s) 377, 485, 560
Hpy188I TCNGA 9 cut(s) 154, 184, 251, 269, 335, 352, 394, 459, 480
Hpy188III TCNNGA 1 cut(s) 407
Hpy99I CGWCG 3 cut(s) 284, 309, 535
HpyAV CCTTC 2 cut(s) 53, 434
HpyCH4III ACNGT 2 cut(s) 158, 548
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 2 cut(s) 301, 524
HpyF10VI GCNNNNNNNGC 4 cut(s) 282, 291, 567, 623
HpyF3I CTNAG 4 cut(s) 229, 266, 458, 595
HpySE526I ACGT 1 cut(s) 279
Hsp92II CATG 1 cut(s) 656
HspAI GCGC 1 cut(s) 74
KpnI GGTACC 1 cut(s) 447
Kzo9I GATC 7 cut(s) 184, 330, 340, 352, 394, 454, 475
LmnI GCTCC 4 cut(s) 71, 96, 416, 748
Lsp1109I GCAGC 3 cut(s) 285, 313, 523
MaeI CTAG 2 cut(s) 18, 638
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 2 cut(s) 199, 548
MalI GATC 7 cut(s) 186, 332, 342, 354, 396, 456, 477
MbiI CCGCTC 1 cut(s) 743
MboI GATC 7 cut(s) 184, 330, 340, 352, 394, 454, 475
MboII GAAGA 1 cut(s) 31
MhlI GDGCHC 2 cut(s) 152, 384
MlsI TGGCCA 1 cut(s) 11
MluCI AATT 3 cut(s) 31, 450, 754
MluNI TGGCCA 1 cut(s) 11
MmeI TCCRAC 1 cut(s) 358
MnlI CCTC 6 cut(s) 91, 464, 569, 634, 654, 715
Mox20I TGGCCA 1 cut(s) 11
MscI TGGCCA 1 cut(s) 11
MseI TTAA 1 cut(s) 784
Msp20I TGGCCA 1 cut(s) 11
MspI CCGG 5 cut(s) 95, 208, 218, 318, 447
MspR9I CCNGG 3 cut(s) 95, 196, 513
MvaI CCWGG 2 cut(s) 196, 513
MwoI GCNNNNNNNGC 4 cut(s) 282, 291, 567, 623
NciI CCSGG 1 cut(s) 95
NdeII GATC 7 cut(s) 184, 330, 340, 352, 394, 454, 475
NheI GCTAGC 1 cut(s) 17
NlaIII CATG 1 cut(s) 656
NlaIV GGNNCC 5 cut(s) 92, 144, 349, 445, 501
NmeAIII GCCGAG 1 cut(s) 363
NmuCI GTSAC 2 cut(s) 199, 548
NspI RCATGY 1 cut(s) 656
PcsI WCGNNNNNNNCGW 1 cut(s) 536
PfeI GAWTC 1 cut(s) 466
PfoI TCCNGGA 1 cut(s) 93
PkrI GCNGC 7 cut(s) 78, 300, 303, 389, 538, 557, 560
Psp6I CCWGG 2 cut(s) 194, 511
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 2 cut(s) 194, 511
PspN4I GGNNCC 5 cut(s) 92, 144, 349, 445, 501
PspPI GGNCC 6 cut(s) 143, 205, 348, 615, 643, 769
PstI CTGCAG 1 cut(s) 303
RsaI GTAC 1 cut(s) 445
RsaNI GTAC 1 cut(s) 444
Rsr2I CGGWCCG 1 cut(s) 205
RsrII CGGWCCG 1 cut(s) 205
SaqAI TTAA 1 cut(s) 784
SatI GCNGC 7 cut(s) 77, 299, 302, 388, 537, 556, 559
Sau3AI GATC 7 cut(s) 184, 330, 340, 352, 394, 454, 475
Sau96I GGNCC 6 cut(s) 143, 205, 348, 615, 643, 769
ScrFI CCNGG 3 cut(s) 95, 196, 513
SduI GDGCHC 2 cut(s) 152, 384
SfcI CTRYAG 1 cut(s) 299
SinI GGWCC 2 cut(s) 205, 348
Sse9I AATT 3 cut(s) 31, 450, 754
SsiI CCGC 5 cut(s) 77, 387, 555, 558, 741
SspMI CTAG 2 cut(s) 18, 638
StyD4I CCNGG 3 cut(s) 93, 194, 511
StyI CCWWGG 1 cut(s) 80
TaaI ACNGT 2 cut(s) 158, 548
TaiI ACGT 1 cut(s) 282
TaqI TCGA 3 cut(s) 187, 307, 469
TasI AATT 3 cut(s) 31, 450, 754
TauI GCSGC 4 cut(s) 79, 390, 558, 561
TfiI GAWTC 1 cut(s) 466
Tru1I TTAA 1 cut(s) 784
Tru9I TTAA 1 cut(s) 784
TscAI CASTG 3 cut(s) 19, 163, 292
TseFI GTSAC 2 cut(s) 199, 548
TseI GCWGC 3 cut(s) 298, 301, 536
Tsp45I GTSAC 2 cut(s) 199, 548
TspDTI ATGAA 2 cut(s) 418, 767
TspGWI ACGGA 1 cut(s) 218
TspRI CASTG 3 cut(s) 19, 163, 292
VpaK11BI GGWCC 2 cut(s) 205, 348
XceI RCATGY 1 cut(s) 656
XcmI CCANNNNNNNNNTGG 1 cut(s) 753
XspI CTAG 2 cut(s) 18, 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.