RLG00000021148

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0019386
Physical Location & Seq
Forward (+)
72337891 .. 72338552
662 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021148

Sequence Viewer

Length: 555 bp
ATGCCAACCCAACCCACATTCTTTGTTACCCGCACCCGCACCCTCACCTCCACCCAAGCCCTTCCCCTCCTCCACTACTCCTCTCCATCCCTTCCTCTTGGCCGCTCCATCTCCTCGTCGATTTTCGACGAGGAGGAGTTGAGCTACTTTATGCCTAAGTTGCTTGCTCTCATAATGACCCTTTCTTTCTCGGTCAAGAAGGTTCTCTATGTCGGGTGGAGGCTCGAGAATCTGCTGGCTTTCGACAAAGGTGTCATCACGACGCCAGAGAATTTTACCTCGACCAGCACCAGCGTCGTGCTAAACATCGGCACTGTTGCTTGGGTCTCTGTCAAGAAAGGCCCTGGTAGTTCTATTTTGATAAATGGCTACTCCTTCTGCCGTCTAACCCACGCCATGGTCATGCTGTTGAAGACCGGAGTTTCCGGATGTGAGGCGAAGGACACTCTGGCTACATCACCGCGATTAGTTCGTCTCCTCAATCTTCTTCTCACGAAGGACATGTACTCCGATTTCGAGGGAATATTCAGTTACATTAAGGGTGCGTTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

20.26

Weight (kDa)

9.3

Isoelectric Point (pI)

32.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019386)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0105281
rosa_laevigata RLG00000021148
rosa_multiflora Rmu_sc0000994.1_g000010
rosa_roxburghii Rroxscaffold_2G00090650 Rroxscaffold_4G00302920
rosa_samantha Rh2CG439100 Rh7AG285600 Rh7CG304600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AccB7I CCANNNNNTGG 1 cut(s) 397
AccBSI CCGCTC 1 cut(s) 105
AccII CGCG 1 cut(s) 463
AccIII TCCGGA 1 cut(s) 425
AciI CCGC 4 cut(s) 31, 37, 103, 461
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 1 cut(s) 271
AcyI GRCGYC 1 cut(s) 263
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 1 cut(s) 397
AflIII ACRYGT 1 cut(s) 501
AgsI TTSAA 1 cut(s) 412
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 1 cut(s) 144
AluI AGCT 1 cut(s) 144
Alw26I GTCTC 2 cut(s) 331, 479
Ama87I CYCGRG 1 cut(s) 224
Aor13HI TCCGGA 1 cut(s) 425
AoxI GGCC 2 cut(s) 100, 340
ApoI RAATTY 1 cut(s) 271
AspS9I GGNCC 1 cut(s) 341
AsuHPI GGTGA 2 cut(s) 37, 450
AvaI CYCGRG 1 cut(s) 224
BarI GAAGNNNNNNTAC 2 cut(s) 488, 520
BbsI GAAGAC 1 cut(s) 419
BccI CCATC 2 cut(s) 94, 116
BceAI ACGGC 1 cut(s) 366
BcgI CGANNNNNNTGC 2 cut(s) 277, 311
BciT130I CCWGG 1 cut(s) 345
BcoDI GTCTC 2 cut(s) 331, 479
BisI GCNGC 1 cut(s) 103
BlsI GCNGC 1 cut(s) 104
Bme1390I CCNGG 1 cut(s) 345
BmeT110I CYCGRG 1 cut(s) 224
BmgT120I GGNCC 1 cut(s) 341
BmrFI CCNGG 1 cut(s) 345
BpiI GAAGAC 1 cut(s) 419
BsaHI GRCGYC 1 cut(s) 263
BsaI GGTCTC 1 cut(s) 331
BsaJI CCNNGG 2 cut(s) 343, 396
BsaWI WCCGGW 2 cut(s) 416, 425
BsaXI ACNNNNNCTCC 2 cut(s) 491, 521
Bsc4I CCNNNNNNNGG 1 cut(s) 397
BseAI TCCGGA 1 cut(s) 425
BseBI CCWGG 1 cut(s) 345
BseDI CCNNGG 2 cut(s) 343, 396
BseGI GGATG 2 cut(s) 86, 434
BseLI CCNNNNNNNGG 1 cut(s) 397
BseRI GAGGAG 6 cut(s) 59, 70, 103, 146, 149, 467
Bsh1236I CGCG 1 cut(s) 463
BshFI GGCC 2 cut(s) 102, 342
BsiHKCI CYCGRG 1 cut(s) 224
BsiSI CCGG 2 cut(s) 417, 426
BslI CCNNNNNNNGG 1 cut(s) 397
BsmAI GTCTC 2 cut(s) 331, 479
BsmBI CGTCTC 1 cut(s) 479
BsnI GGCC 2 cut(s) 102, 342
Bso31I GGTCTC 1 cut(s) 331
BsoBI CYCGRG 1 cut(s) 224
Bsp13I TCCGGA 1 cut(s) 425
Bsp19I CCATGG 1 cut(s) 396
BspACI CCGC 4 cut(s) 31, 37, 103, 461
BspANI GGCC 2 cut(s) 102, 342
BspEI TCCGGA 1 cut(s) 425
BspFNI CGCG 1 cut(s) 463
BspTNI GGTCTC 1 cut(s) 331
BsrBI CCGCTC 1 cut(s) 105
BssECI CCNNGG 2 cut(s) 343, 396
BssNI GRCGYC 1 cut(s) 263
BssT1I CCWWGG 1 cut(s) 396
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 1 cut(s) 316
BstACI GRCGYC 1 cut(s) 263
BstC8I GCNNGC 2 cut(s) 165, 237
BstDEI CTNAG 1 cut(s) 156
BstDSI CCRYGG 1 cut(s) 396
BstF5I GGATG 2 cut(s) 86, 434
BstFNI CGCG 1 cut(s) 463
BstMAI GTCTC 2 cut(s) 331, 479
BstMWI GCNNNNNNNGC 1 cut(s) 160
BstNI CCWGG 1 cut(s) 345
BstNSI RCATGY 1 cut(s) 505
BstSCI CCNGG 1 cut(s) 343
BstUI CGCG 1 cut(s) 463
BstV2I GAAGAC 1 cut(s) 419
BsuRI GGCC 2 cut(s) 102, 342
BtgI CCRYGG 1 cut(s) 396
BtsCI GGATG 2 cut(s) 86, 434
BtsIMutI CAGTG 1 cut(s) 312
Cac8I GCNNGC 2 cut(s) 165, 237
Cfr13I GGNCC 1 cut(s) 341
CseI GACGC 2 cut(s) 271, 283
Csp6I GTAC 1 cut(s) 505
CspCI CAANNNNNGTGG 2 cut(s) 4, 39
CviAII CATG 3 cut(s) 397, 403, 502
CviJI RGCY 8 cut(s) 59, 102, 144, 223, 239, 342, 369, 452
CviKI_1 RGCY 8 cut(s) 59, 102, 144, 223, 239, 342, 369, 452
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 1 cut(s) 156
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
EaeI YGGCCR 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 396
Eco31I GGTCTC 1 cut(s) 331
Eco88I CYCGRG 1 cut(s) 224
EcoO109I RGGNCCY 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 343
EcoT14I CCWWGG 1 cut(s) 396
ErhI CCWWGG 1 cut(s) 396
Esp3I CGTCTC 1 cut(s) 479
FaeI CATG 3 cut(s) 400, 406, 505
FaiI YATR 6 cut(s) 152, 173, 210, 398, 404, 503
FatI CATG 3 cut(s) 396, 402, 501
FauI CCCGC 2 cut(s) 38, 44
Fnu4HI GCNGC 1 cut(s) 103
FokI GGATG 2 cut(s) 73, 441
Fsp4HI GCNGC 1 cut(s) 103
GluI GCNGC 1 cut(s) 103
HaeIII GGCC 2 cut(s) 102, 342
HapII CCGG 2 cut(s) 417, 426
HgaI GACGC 2 cut(s) 271, 283
Hin1I GRCGYC 1 cut(s) 263
Hin1II CATG 3 cut(s) 400, 406, 505
HinfI GANTC 1 cut(s) 229
HpaII CCGG 2 cut(s) 417, 426
HphI GGTGA 2 cut(s) 37, 450
Hpy188I TCNGA 1 cut(s) 511
Hpy188III TCNNGA 6 cut(s) 196, 226, 259, 334, 426, 493
Hpy99I CGWCG 4 cut(s) 121, 131, 265, 299
HpyAV CCTTC 6 cut(s) 71, 101, 193, 385, 433, 490
HpyCH4III ACNGT 1 cut(s) 316
HpyF10VI GCNNNNNNNGC 1 cut(s) 160
HpyF3I CTNAG 1 cut(s) 156
Hsp92I GRCGYC 1 cut(s) 263
Hsp92II CATG 3 cut(s) 400, 406, 505
Kpn2I TCCGGA 1 cut(s) 425
LmnI GCTCC 1 cut(s) 110
LpnPI CCDG 9 cut(s) 221, 279, 298, 304, 330, 357, 430, 434, 439
MaeIII GTNAC 2 cut(s) 25, 530
MbiI CCGCTC 1 cut(s) 105
MboII GAAGA 3 cut(s) 424, 476, 479
MluCI AATT 1 cut(s) 271
MroI TCCGGA 1 cut(s) 425
MseI TTAA 1 cut(s) 537
MslI CAYNNNNRTG 1 cut(s) 401
MspI CCGG 2 cut(s) 417, 426
MspR9I CCNGG 1 cut(s) 345
MvaI CCWGG 1 cut(s) 345
MvnI CGCG 1 cut(s) 463
MwoI GCNNNNNNNGC 1 cut(s) 160
NcoI CCATGG 1 cut(s) 396
NlaIII CATG 3 cut(s) 400, 406, 505
NspI RCATGY 1 cut(s) 505
PaeR7I CTCGAG 1 cut(s) 224
PciI ACATGT 1 cut(s) 501
PfeI GAWTC 1 cut(s) 229
PflMI CCANNNNNTGG 1 cut(s) 397
PkrI GCNGC 1 cut(s) 104
PscI ACATGT 1 cut(s) 501
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspPI GGNCC 1 cut(s) 341
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
RseI CAYNNNNRTG 1 cut(s) 401
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 1 cut(s) 103
Sau96I GGNCC 1 cut(s) 341
ScrFI CCNGG 1 cut(s) 345
SetI ASST 5 cut(s) 50, 146, 204, 253, 281
Sfr274I CTCGAG 1 cut(s) 224
SlaI CTCGAG 1 cut(s) 224
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
Sse9I AATT 1 cut(s) 271
SsiI CCGC 4 cut(s) 31, 37, 103, 461
SspI AATATT 1 cut(s) 525
StyD4I CCNGG 1 cut(s) 343
StyI CCWWGG 1 cut(s) 396
TaaI ACNGT 1 cut(s) 316
TaqI TCGA 6 cut(s) 119, 126, 225, 243, 281, 516
TaqII GACCGA 1 cut(s) 181
TasI AATT 1 cut(s) 271
TatI WGTACW 1 cut(s) 504
TauI GCSGC 1 cut(s) 105
TfiI GAWTC 1 cut(s) 229
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TscAI CASTG 1 cut(s) 319
TspRI CASTG 1 cut(s) 319
Van91I CCANNNNNTGG 1 cut(s) 397
XapI RAATTY 1 cut(s) 271
XceI RCATGY 1 cut(s) 505
XhoI CTCGAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.