Rroxscaffold_2G00090650

CRS2-associated factor 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0019386
Physical Location & Seq
Reverse (-)
12356551 .. 12359568
3018 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00090650.1

Sequence Viewer

Length: 945 bp
ATGCCCCATTTTCACGGCACTGTGATCCCGAACGACCCACAATCCACTCCCGAACCGCCGCCCACGGAGGTCGATTCCCTCTGTGGAATGCCAACGTCGAGAAATTCAACCGGAGCCGCCGCGCCTAGCAAGAAATCGGGGATGACGTCCGCCGTGAGTGCTACTTCGACTCCATCACCGTCGCGCGACGACTCCTCCTTCGATGCCAAAGCAACATTCAAATCCATAGGTACGCCTTCATCTCCCTCAAGCCCTTCAATTCCGGGCGAGAAAATCCAAGTACTCCAAGAACTCGACCCTAGCTCACACCCGGTGTTTCACCGATTCATCGGACCCACGAAGCTCTCAAGTATCTTTAGAGAGAAGTCGGAAATCAACAGGAAAGTCAACATCTCCATTGGCGACGACGGGCTCTCTTTGTGTTCGATCAAATGCACGAAAGATGGTTTTGCATGGACTACAATGTTTTCTTCTCTTACTCGTGTAGGGAATATGATTTTGGCGAGGACATTGTTCGATGAGATGATAGAAAGGTGTATAGCTACTTGGAATACTATGATCGACGACGTGAAAGGTGCGAGAGAAGTGAAACCGAAGAGAGAGAACAAAGGGCTCGAGAATCTACTAGCCTTCGGCGAAGGTGTCATCACAATGCCAGAGAATTTTACCTCGAGCAACACTAGCCTCGTGCCAAACATCTGCACCGTTGCTTGGGTCTCTGTCAAGAAAGGTCCTGGTAGTTCTATTGTTATCAATGGCTACTCCTTCTGCCGTCTAAGCCACGCCATGGTCATGCTGTTGAAGACCAGAGTTTCTGGATGTGAGGCGAAGGACATTCTGGCTACATCACCGCAATTCGTTCGTCTCCTCAATCTTCCTCTCACGAAGGACATGTACTCCGATTTCGAGAGAATATTCAGTTACATTAAGGGTGCATTTGGATGA

Protein Analysis

314

Amino Acids

34.05

Weight (kDa)

8.63

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019386)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0105281
rosa_laevigata RLG00000021148
rosa_multiflora Rmu_sc0000994.1_g000010
rosa_roxburghii Rroxscaffold_2G00090650 Rroxscaffold_4G00302920
rosa_samantha Rh2CG439100 Rh7AG285600 Rh7CG304600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 149
AccB7I CCANNNNNTGG 1 cut(s) 787
AccII CGCG 3 cut(s) 122, 184, 186
AciI CCGC 6 cut(s) 56, 59, 117, 120, 150, 851
AclWI GGATC 1 cut(s) 19
AcsI RAATTY 2 cut(s) 103, 661
AcyI GRCGYC 1 cut(s) 146
AdeI CACNNNGTG 1 cut(s) 313
AfaI GTAC 3 cut(s) 232, 282, 896
AfiI CCNNNNNNNGG 2 cut(s) 711, 787
AflIII ACRYGT 1 cut(s) 891
AgsI TTSAA 4 cut(s) 108, 220, 258, 802
AjiI CACGTC 1 cut(s) 568
AjnI CCWGG 1 cut(s) 733
AjuI GAANNNNNNNTTGG 4 cut(s) 200, 232, 482, 514
AluBI AGCT 3 cut(s) 303, 343, 542
AluI AGCT 3 cut(s) 303, 343, 542
Alw26I GTCTC 2 cut(s) 721, 869
AlwI GGATC 1 cut(s) 19
Ama87I CYCGRG 2 cut(s) 614, 670
ApoI RAATTY 2 cut(s) 103, 661
ArsI GACNNNNNNTTYG 2 cut(s) 182, 214
AspLEI GCGC 2 cut(s) 124, 186
AspS9I GGNCC 2 cut(s) 332, 731
AsuC2I CCSGG 2 cut(s) 264, 311
AsuHPI GGTGA 3 cut(s) 168, 311, 840
AvaI CYCGRG 2 cut(s) 614, 670
AvaII GGWCC 2 cut(s) 332, 731
BanII GRGCYC 2 cut(s) 414, 615
BarI GAAGNNNNNNTAC 2 cut(s) 878, 910
BauI CACGAG 2 cut(s) 480, 686
BbsI GAAGAC 1 cut(s) 809
BccI CCATC 2 cut(s) 181, 437
BceAI ACGGC 3 cut(s) 31, 137, 756
BcgI CGANNNNNNTGC 2 cut(s) 842, 876
BciT130I CCWGG 1 cut(s) 735
BcnI CCSGG 2 cut(s) 264, 311
BcoDI GTCTC 2 cut(s) 721, 869
BfaI CTAG 4 cut(s) 126, 300, 626, 681
BisI GCNGC 3 cut(s) 59, 117, 120
BlsI GCNGC 3 cut(s) 60, 118, 121
BmcAI AGTACT 1 cut(s) 282
Bme1390I CCNGG 3 cut(s) 264, 311, 735
Bme18I GGWCC 2 cut(s) 332, 731
BmeT110I CYCGRG 2 cut(s) 614, 670
BmgBI CACGTC 1 cut(s) 568
BmgT120I GGNCC 2 cut(s) 332, 731
BmiI GGNNCC 2 cut(s) 115, 334
BmrFI CCNGG 3 cut(s) 264, 311, 735
BmsI GCATC 1 cut(s) 193
BpiI GAAGAC 1 cut(s) 809
BpuEI CTTGAG 2 cut(s) 232, 331
BpuMI CCSGG 2 cut(s) 264, 311
BsaHI GRCGYC 1 cut(s) 146
BsaI GGTCTC 1 cut(s) 721
BsaJI CCNNGG 2 cut(s) 63, 786
BsaWI WCCGGW 1 cut(s) 110
BsaXI ACNNNNNCTCC 6 cut(s) 154, 179, 184, 209, 881, 911
Bsc4I CCNNNNNNNGG 2 cut(s) 711, 787
BseBI CCWGG 1 cut(s) 735
BseDI CCNNGG 2 cut(s) 63, 786
BseGI GGATG 2 cut(s) 147, 824
BseLI CCNNNNNNNGG 2 cut(s) 711, 787
BseRI GAGGAG 2 cut(s) 184, 857
BsgI GTGCAG 1 cut(s) 685
Bsh1236I CGCG 3 cut(s) 122, 184, 186
BsiHKCI CYCGRG 2 cut(s) 614, 670
BsiSI CCGG 3 cut(s) 111, 263, 311
BslI CCNNNNNNNGG 2 cut(s) 711, 787
BsmAI GTCTC 2 cut(s) 721, 869
BsmBI CGTCTC 1 cut(s) 869
BsmI GAATGC 1 cut(s) 93
Bso31I GGTCTC 1 cut(s) 721
BsoBI CYCGRG 2 cut(s) 614, 670
Bsp1286I GDGCHC 2 cut(s) 414, 615
Bsp143I GATC 3 cut(s) 24, 426, 558
Bsp19I CCATGG 1 cut(s) 786
BspACI CCGC 6 cut(s) 56, 59, 117, 120, 150, 851
BspFNI CGCG 3 cut(s) 122, 184, 186
BspLI GGNNCC 2 cut(s) 115, 334
BspPI GGATC 1 cut(s) 19
BspTNI GGTCTC 1 cut(s) 721
BssECI CCNNGG 2 cut(s) 63, 786
BssMI GATC 3 cut(s) 24, 426, 558
BssNI GRCGYC 1 cut(s) 146
BssSI CACGAG 2 cut(s) 480, 686
BssT1I CCWWGG 1 cut(s) 786
Bst2BI CACGAG 2 cut(s) 480, 686
Bst2UI CCWGG 1 cut(s) 735
Bst4CI ACNGT 3 cut(s) 22, 180, 706
Bst6I CTCTTC 1 cut(s) 590
BstACI GRCGYC 1 cut(s) 146
BstDEI CTNAG 1 cut(s) 776
BstDSI CCRYGG 2 cut(s) 63, 786
BstF5I GGATG 2 cut(s) 147, 824
BstFNI CGCG 3 cut(s) 122, 184, 186
BstHHI GCGC 2 cut(s) 124, 186
BstKTI GATC 3 cut(s) 27, 429, 561
BstMAI GTCTC 2 cut(s) 721, 869
BstMBI GATC 3 cut(s) 24, 426, 558
BstMWI GCNNNNNNNGC 3 cut(s) 158, 681, 777
BstNI CCWGG 1 cut(s) 735
BstNSI RCATGY 1 cut(s) 895
BstSCI CCNGG 3 cut(s) 262, 309, 733
BstUI CGCG 3 cut(s) 122, 184, 186
BstV2I GAAGAC 1 cut(s) 809
BtgI CCRYGG 2 cut(s) 63, 786
BtrI CACGTC 1 cut(s) 568
BtsCI GGATG 2 cut(s) 147, 824
BtsIMutI CAGTG 1 cut(s) 18
CfoI GCGC 2 cut(s) 124, 186
Cfr13I GGNCC 2 cut(s) 332, 731
Csp6I GTAC 3 cut(s) 231, 281, 895
CviAII CATG 4 cut(s) 453, 787, 793, 892
CviQI GTAC 3 cut(s) 231, 281, 895
DdeI CTNAG 1 cut(s) 776
DpnI GATC 3 cut(s) 26, 428, 560
DpnII GATC 3 cut(s) 24, 426, 558
DraIII CACNNNGTG 1 cut(s) 313
Eam1104I CTCTTC 1 cut(s) 590
EarI CTCTTC 1 cut(s) 590
EciI GGCGGA 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 786
Eco24I GRGCYC 2 cut(s) 414, 615
Eco31I GGTCTC 1 cut(s) 721
Eco47I GGWCC 2 cut(s) 332, 731
Eco88I CYCGRG 2 cut(s) 614, 670
EcoO109I RGGNCCY 1 cut(s) 731
EcoRII CCWGG 1 cut(s) 733
EcoT14I CCWWGG 1 cut(s) 786
EcoT38I GRGCYC 2 cut(s) 414, 615
ErhI CCWWGG 1 cut(s) 786
Esp3I CGTCTC 1 cut(s) 869
FaeI CATG 4 cut(s) 456, 790, 796, 895
FaiI YATR 8 cut(s) 227, 454, 494, 539, 557, 788, 794, 893
FatI CATG 4 cut(s) 452, 786, 792, 891
Fnu4HI GCNGC 3 cut(s) 59, 117, 120
FokI GGATG 2 cut(s) 154, 831
FriOI GRGCYC 2 cut(s) 414, 615
Fsp4HI GCNGC 3 cut(s) 59, 117, 120
FspBI CTAG 4 cut(s) 126, 300, 626, 681
GlaI GCGC 2 cut(s) 123, 185
GluI GCNGC 3 cut(s) 59, 117, 120
HapII CCGG 3 cut(s) 111, 263, 311
HhaI GCGC 2 cut(s) 124, 186
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 4 cut(s) 456, 790, 796, 895
Hin6I GCGC 2 cut(s) 122, 184
HinP1I GCGC 2 cut(s) 122, 184
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 5 cut(s) 74, 169, 191, 324, 619
HpaII CCGG 3 cut(s) 111, 263, 311
HphI GGTGA 3 cut(s) 168, 311, 840
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 3 cut(s) 332, 370, 901
Hpy188III TCNNGA 8 cut(s) 28, 50, 99, 616, 724, 816, 883, 907
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 7 cut(s) 100, 184, 191, 407, 410, 566, 569
HpyAV CCTTC 8 cut(s) 208, 246, 264, 632, 640, 775, 823, 880
HpyCH4III ACNGT 3 cut(s) 22, 180, 706
HpyCH4IV ACGT 3 cut(s) 95, 146, 567
HpyCH4V TGCA 4 cut(s) 435, 452, 702, 935
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 681, 777
HpyF3I CTNAG 1 cut(s) 776
HpySE526I ACGT 3 cut(s) 95, 146, 567
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 4 cut(s) 456, 790, 796, 895
HspAI GCGC 2 cut(s) 122, 184
Kzo9I GATC 3 cut(s) 24, 426, 558
LmnI GCTCC 1 cut(s) 113
LweI GCATC 1 cut(s) 193
MaeI CTAG 4 cut(s) 126, 300, 626, 681
MaeII ACGT 3 cut(s) 95, 146, 567
MaeIII GTNAC 1 cut(s) 920
MalI GATC 3 cut(s) 26, 428, 560
MboI GATC 3 cut(s) 24, 426, 558
MboII GAAGA 4 cut(s) 462, 607, 814, 866
MhlI GDGCHC 2 cut(s) 414, 615
MluCI AATT 4 cut(s) 103, 258, 661, 854
MlyI GAGTC 2 cut(s) 163, 185
MmeI TCCRAC 1 cut(s) 348
MseI TTAA 1 cut(s) 927
MslI CAYNNNNRTG 3 cut(s) 650, 791, 940
MspI CCGG 3 cut(s) 111, 263, 311
MspR9I CCNGG 3 cut(s) 264, 311, 735
Mva1269I GAATGC 1 cut(s) 93
MvaI CCWGG 1 cut(s) 735
MvnI CGCG 3 cut(s) 122, 184, 186
MwoI GCNNNNNNNGC 3 cut(s) 158, 681, 777
NciI CCSGG 2 cut(s) 264, 311
NcoI CCATGG 1 cut(s) 786
NdeII GATC 3 cut(s) 24, 426, 558
NlaIII CATG 4 cut(s) 456, 790, 796, 895
NlaIV GGNNCC 2 cut(s) 115, 334
NspI RCATGY 1 cut(s) 895
PaeR7I CTCGAG 2 cut(s) 614, 670
PciI ACATGT 1 cut(s) 891
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PctI GAATGC 1 cut(s) 93
PfeI GAWTC 3 cut(s) 74, 324, 619
PflMI CCANNNNNTGG 1 cut(s) 787
PkrI GCNGC 3 cut(s) 60, 118, 121
PleI GAGTC 2 cut(s) 163, 185
PpsI GAGTC 2 cut(s) 163, 185
PpuMI RGGWCCY 1 cut(s) 731
PscI ACATGT 1 cut(s) 891
Psp5II RGGWCCY 1 cut(s) 731
Psp6I CCWGG 1 cut(s) 733
PspGI CCWGG 1 cut(s) 733
PspN4I GGNNCC 2 cut(s) 115, 334
PspPI GGNCC 2 cut(s) 332, 731
PspPPI RGGWCCY 1 cut(s) 731
PspXI VCTCGAGB 1 cut(s) 670
RsaI GTAC 3 cut(s) 232, 282, 896
RsaNI GTAC 3 cut(s) 231, 281, 895
RseI CAYNNNNRTG 3 cut(s) 650, 791, 940
SaqAI TTAA 1 cut(s) 927
SatI GCNGC 3 cut(s) 59, 117, 120
Sau3AI GATC 3 cut(s) 24, 426, 558
Sau96I GGNCC 2 cut(s) 332, 731
ScaI AGTACT 1 cut(s) 282
SchI GAGTC 2 cut(s) 163, 185
ScrFI CCNGG 3 cut(s) 264, 311, 735
SduI GDGCHC 2 cut(s) 414, 615
SfaNI GCATC 1 cut(s) 193
Sfr274I CTCGAG 2 cut(s) 614, 670
SinI GGWCC 2 cut(s) 332, 731
SlaI CTCGAG 2 cut(s) 614, 670
SmiMI CAYNNNNRTG 3 cut(s) 650, 791, 940
SmlI CTYRAG 4 cut(s) 247, 346, 614, 670
SmoI CTYRAG 4 cut(s) 247, 346, 614, 670
Sse9I AATT 4 cut(s) 103, 258, 661, 854
SsiI CCGC 6 cut(s) 56, 59, 117, 120, 150, 851
SspI AATATT 1 cut(s) 915
SspMI CTAG 4 cut(s) 126, 300, 626, 681
StyD4I CCNGG 3 cut(s) 262, 309, 733
StyI CCWWGG 1 cut(s) 786
TaaI ACNGT 3 cut(s) 22, 180, 706
TaiI ACGT 3 cut(s) 98, 149, 570
TasI AATT 4 cut(s) 103, 258, 661, 854
TatI WGTACW 2 cut(s) 280, 894
TauI GCSGC 3 cut(s) 61, 119, 122
TfiI GAWTC 3 cut(s) 74, 324, 619
Tru1I TTAA 1 cut(s) 927
Tru9I TTAA 1 cut(s) 927
TscAI CASTG 1 cut(s) 25
TspDTI ATGAA 2 cut(s) 228, 316
TspGWI ACGGA 1 cut(s) 80
TspRI CASTG 1 cut(s) 25
Van91I CCANNNNNTGG 1 cut(s) 787
VpaK11BI GGWCC 2 cut(s) 332, 731
XapI RAATTY 2 cut(s) 103, 661
XceI RCATGY 1 cut(s) 895
XhoI CTCGAG 2 cut(s) 614, 670
XspI CTAG 4 cut(s) 126, 300, 626, 681
ZraI GACGTC 1 cut(s) 147
ZrmI AGTACT 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.