RLG00000021349

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018064
Physical Location & Seq
Forward (+)
74364058 .. 74365227
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021349

Sequence Viewer

Length: 813 bp
ATGAACGGTCCAGCTCGAAACACCACAGTGAAGCGTCTGAGCCGGCGGTTCAATGGCTATGAGCCTTTGGATGAAGCGATGGGAAGCGAAAACGTGCAAGACCCGCCGCGGAGATCGATGTCGTTTAAGCGCAAGAAAGTGGCGGCTTTTCCTTTGAGGACTAGAGAGGATGAAGCCAAGCAACGACGGCAGATATTCCTTAACTCCTACAAGTTTGAAACGGCGGATAAAACGCAGCGGTCGGTGTGTAGTCGGAAGATGAACAAGGTGGTTTGTAAGTTCAAGACATCAGCAGCGAAGCTCGTGTCATCGTTTTTGGGAGCTGGTGCTTTCAGATCTTGTAATTGTCGTCTGGCCATTCAAGCTTTCTCTCCTAGACCCCGTAGCAACGCTAGGGGTTTCTTCGAAAAATTTTCTTCTCCGTCTGACGGCGCGTCCATGGACGCTGTTGTCGGACGGGCTTTGTTGGCAGGTGCTTGTGGTAGGAGGGAGGTGGTAGGAGAGTTTAAGATCACTGGAGGGTTGGGTTTCGGATATGGCATGCCAAGGTTGTGGATCAAACTTGCATCGGAGGATCGGTTTTCAGTCACTAGTTTGGGTTCGGAGGAGACAGCAGGCTGTGGGGCGAGGATTCGAGAAGGTGGGCCGGCCTCTGGCTGGTGCAAGGAAGACGGCAGCACGATGGTGGTTGTTCAACCGAGCACCGCAGTACGTGTTGGTGGCGGCTTTTATGGATCTGGGCCTGGCTTGTCTAGAGGGCTTGGGCATTCTCCTTGCTCCCTTTTTTGGGCTTGGGCCTGGGATTTACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

271

Amino Acids

29.42

Weight (kDa)

10.11

Isoelectric Point (pI)

49.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018064)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45100
malus_domestica MD09G1089500.v1.1
prunus_persica Prupe.3G235900_v2.0.a1
rosa_laevigata RLG00000021349
rosa_multiflora Rmu_sc0004206.1_g000013
rosa_roxburghii Rroxscaffold_2G00087880
rosa_rugosa Rorug02G0498200
rosa_samantha Rh2AG564100 Rh2BG576700 Rh2CG546700 Rh2DG586500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 461
AasI GACNNNNNNGTC 1 cut(s) 449
Acc36I ACCTGC 1 cut(s) 461
AccII CGCG 2 cut(s) 109, 434
AciI CCGC 9 cut(s) 46, 104, 107, 109, 143, 224, 238, 705, 723
AclWI GGATC 3 cut(s) 563, 582, 742
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 1 cut(s) 410
AfaI GTAC 1 cut(s) 711
AfiI CCNNNNNNNGG 5 cut(s) 428, 653, 657, 786, 787
AflIII ACRYGT 1 cut(s) 712
AgsI TTSAA 5 cut(s) 52, 218, 283, 362, 695
AhdI GACNNNNNGTC 1 cut(s) 433
AhlI ACTAGT 1 cut(s) 590
AjnI CCWGG 2 cut(s) 742, 797
AleI CACNNNNGTG 2 cut(s) 26, 683
AluBI AGCT 4 cut(s) 14, 301, 323, 365
AluI AGCT 4 cut(s) 14, 301, 323, 365
Alw21I GWGCWC 1 cut(s) 704
Alw26I GTCTC 1 cut(s) 602
AlwI GGATC 3 cut(s) 563, 582, 742
AoxI GGCC 5 cut(s) 354, 644, 648, 740, 795
ApeKI GCWGC 3 cut(s) 235, 293, 675
ApoI RAATTY 1 cut(s) 410
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
AspLEI GCGC 2 cut(s) 132, 434
AspS9I GGNCC 4 cut(s) 8, 644, 740, 795
AsuII TTCGAA 1 cut(s) 405
AvaII GGWCC 1 cut(s) 8
BalI TGGCCA 1 cut(s) 356
BauI CACGAG 1 cut(s) 302
BbsI GAAGAC 1 cut(s) 675
Bbv12I GWGCWC 1 cut(s) 704
BbvI GCAGC 3 cut(s) 247, 305, 687
BccI CCATC 2 cut(s) 73, 676
BceAI ACGGC 4 cut(s) 203, 237, 445, 688
BciT130I CCWGG 2 cut(s) 744, 799
BcoDI GTCTC 1 cut(s) 602
BcuI ACTAGT 1 cut(s) 590
BfaI CTAG 5 cut(s) 162, 375, 393, 591, 753
BfuAI ACCTGC 1 cut(s) 461
BglII AGATCT 1 cut(s) 335
BisI GCNGC 6 cut(s) 107, 144, 236, 294, 676, 724
BlsI GCNGC 6 cut(s) 108, 145, 237, 295, 677, 725
Bme1390I CCNGG 2 cut(s) 744, 799
Bme18I GGWCC 1 cut(s) 8
BmeRI GACNNNNNGTC 1 cut(s) 433
BmgT120I GGNCC 4 cut(s) 8, 644, 740, 795
BmrFI CCNGG 2 cut(s) 744, 799
BmsI GCATC 1 cut(s) 575
BpiI GAAGAC 1 cut(s) 675
BpmI CTGGAG 1 cut(s) 537
Bpu14I TTCGAA 1 cut(s) 405
Bsa29I ATCGAT 1 cut(s) 116
BsaAI YACGTR 1 cut(s) 713
BsaJI CCNNGG 4 cut(s) 107, 438, 545, 798
BsaXI ACNNNNNCTCC 2 cut(s) 563, 593
Bsc4I CCNNNNNNNGG 5 cut(s) 428, 653, 657, 786, 787
Bse118I RCCGGY 2 cut(s) 42, 646
Bse1I ACTGG 1 cut(s) 520
BseBI CCWGG 2 cut(s) 744, 799
BseCI ATCGAT 1 cut(s) 116
BseDI CCNNGG 4 cut(s) 107, 438, 545, 798
BseGI GGATG 2 cut(s) 76, 175
BseLI CCNNNNNNNGG 5 cut(s) 428, 653, 657, 786, 787
BseMII CTCAG 1 cut(s) 29
BseNI ACTGG 1 cut(s) 520
BseRI GAGGAG 1 cut(s) 620
BseXI GCAGC 3 cut(s) 247, 305, 687
Bsh1236I CGCG 2 cut(s) 109, 434
Bsh1285I CGRYCG 1 cut(s) 242
BshFI GGCC 5 cut(s) 356, 646, 650, 742, 797
BshVI ATCGAT 1 cut(s) 116
BsiEI CGRYCG 1 cut(s) 242
BsiHKAI GWGCWC 1 cut(s) 704
BsiSI CCGG 2 cut(s) 43, 647
BslI CCNNNNNNNGG 5 cut(s) 428, 653, 657, 786, 787
BsmAI GTCTC 1 cut(s) 602
BsmI GAATGC 1 cut(s) 766
BsnI GGCC 5 cut(s) 356, 646, 650, 742, 797
Bsp119I TTCGAA 1 cut(s) 405
Bsp1286I GDGCHC 1 cut(s) 704
Bsp143I GATC 6 cut(s) 113, 335, 510, 555, 574, 734
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 9 cut(s) 46, 104, 107, 109, 143, 224, 238, 705, 723
BspANI GGCC 5 cut(s) 356, 646, 650, 742, 797
BspCNI CTCAG 1 cut(s) 30
BspDI ATCGAT 1 cut(s) 116
BspFNI CGCG 2 cut(s) 109, 434
BspMI ACCTGC 1 cut(s) 461
BspPI GGATC 3 cut(s) 563, 582, 742
BspT104I TTCGAA 1 cut(s) 405
BsrFI RCCGGY 2 cut(s) 42, 646
BsrI ACTGG 1 cut(s) 520
BssAI RCCGGY 2 cut(s) 42, 646
BssECI CCNNGG 4 cut(s) 107, 438, 545, 798
BssMI GATC 6 cut(s) 113, 335, 510, 555, 574, 734
BssSI CACGAG 1 cut(s) 302
BssT1I CCWWGG 2 cut(s) 438, 545
Bst2BI CACGAG 1 cut(s) 302
Bst2UI CCWGG 2 cut(s) 744, 799
Bst4CI ACNGT 2 cut(s) 8, 28
BstBAI YACGTR 1 cut(s) 713
BstBI TTCGAA 1 cut(s) 405
BstC8I GCNNGC 4 cut(s) 44, 542, 616, 648
BstDEI CTNAG 1 cut(s) 38
BstDSI CCRYGG 2 cut(s) 107, 438
BstF5I GGATG 2 cut(s) 76, 175
BstFNI CGCG 2 cut(s) 109, 434
BstHHI GCGC 2 cut(s) 132, 434
BstKTI GATC 6 cut(s) 116, 338, 513, 558, 577, 737
BstMAI GTCTC 1 cut(s) 602
BstMBI GATC 6 cut(s) 113, 335, 510, 555, 574, 734
BstMCI CGRYCG 1 cut(s) 242
BstMWI GCNNNNNNNGC 4 cut(s) 103, 187, 362, 467
BstNI CCWGG 2 cut(s) 744, 799
BstNSI RCATGY 1 cut(s) 544
BstSCI CCNGG 2 cut(s) 742, 797
BstUI CGCG 2 cut(s) 109, 434
BstV1I GCAGC 3 cut(s) 247, 305, 687
BstV2I GAAGAC 1 cut(s) 675
BstX2I RGATCY 2 cut(s) 335, 734
BstXI CCANNNNNNTGG 1 cut(s) 552
BstYI RGATCY 2 cut(s) 335, 734
Bsu15I ATCGAT 1 cut(s) 116
BsuRI GGCC 5 cut(s) 356, 646, 650, 742, 797
BsuTUI ATCGAT 1 cut(s) 116
BtgI CCRYGG 2 cut(s) 107, 438
BtgZI GCGATG 1 cut(s) 92
BtsCI GGATG 2 cut(s) 76, 175
BtsIMutI CAGTG 2 cut(s) 33, 513
BveI ACCTGC 1 cut(s) 461
Cac8I GCNNGC 4 cut(s) 44, 542, 616, 648
CfoI GCGC 2 cut(s) 132, 434
Cfr10I RCCGGY 2 cut(s) 42, 646
Cfr13I GGNCC 4 cut(s) 8, 644, 740, 795
Cfr42I CCGCGG 1 cut(s) 110
ClaI ATCGAT 1 cut(s) 116
CseI GACGC 3 cut(s) 23, 423, 452
Csp6I GTAC 1 cut(s) 710
CviAII CATG 2 cut(s) 439, 541
CviQI GTAC 1 cut(s) 710
DdeI CTNAG 1 cut(s) 38
DpnI GATC 6 cut(s) 115, 337, 512, 557, 576, 736
DpnII GATC 6 cut(s) 113, 335, 510, 555, 574, 734
DrdI GACNNNNNNGTC 1 cut(s) 449
DriI GACNNNNNGTC 1 cut(s) 433
DseDI GACNNNNNNGTC 1 cut(s) 449
EaeI YGGCCR 1 cut(s) 354
Eam1105I GACNNNNNGTC 1 cut(s) 433
EciI GGCGGA 1 cut(s) 239
Eco130I CCWWGG 2 cut(s) 438, 545
Eco47I GGWCC 1 cut(s) 8
EcoRII CCWGG 2 cut(s) 742, 797
EcoT14I CCWWGG 2 cut(s) 438, 545
ErhI CCWWGG 2 cut(s) 438, 545
FaeI CATG 2 cut(s) 442, 544
FaiI YATR 5 cut(s) 60, 440, 537, 542, 732
FatI CATG 2 cut(s) 438, 540
FauI CCCGC 1 cut(s) 111
Fnu4HI GCNGC 6 cut(s) 107, 144, 236, 294, 676, 724
FokI GGATG 2 cut(s) 83, 182
FseI GGCCGGCC 1 cut(s) 650
Fsp4HI GCNGC 6 cut(s) 107, 144, 236, 294, 676, 724
FspBI CTAG 5 cut(s) 162, 375, 393, 591, 753
GlaI GCGC 2 cut(s) 131, 433
GluI GCNGC 6 cut(s) 107, 144, 236, 294, 676, 724
GsuI CTGGAG 1 cut(s) 537
HaeIII GGCC 5 cut(s) 356, 646, 650, 742, 797
HapII CCGG 2 cut(s) 43, 647
HgaI GACGC 3 cut(s) 23, 423, 452
HhaI GCGC 2 cut(s) 132, 434
Hin1II CATG 2 cut(s) 442, 544
Hin6I GCGC 2 cut(s) 130, 432
HinP1I GCGC 2 cut(s) 130, 432
HindIII AAGCTT 1 cut(s) 363
HinfI GANTC 1 cut(s) 631
HpaII CCGG 2 cut(s) 43, 647
Hpy188I TCNGA 8 cut(s) 39, 255, 335, 427, 455, 533, 571, 604
Hpy188III TCNNGA 3 cut(s) 283, 635, 753
Hpy99I CGWCG 1 cut(s) 189
HpyAV CCTTC 1 cut(s) 632
HpyCH4III ACNGT 2 cut(s) 8, 28
HpyCH4IV ACGT 2 cut(s) 93, 712
HpyCH4V TGCA 3 cut(s) 97, 566, 663
HpyF10VI GCNNNNNNNGC 4 cut(s) 103, 187, 362, 467
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 2 cut(s) 93, 712
Hsp92II CATG 2 cut(s) 442, 544
HspAI GCGC 2 cut(s) 130, 432
KroI GCCGGC 2 cut(s) 42, 646
KroNI GCCGGC 2 cut(s) 44, 648
KspI CCGCGG 1 cut(s) 110
Kzo9I GATC 6 cut(s) 113, 335, 510, 555, 574, 734
LmnI GCTCC 2 cut(s) 320, 782
Lsp1109I GCAGC 3 cut(s) 247, 305, 687
LweI GCATC 1 cut(s) 575
MaeI CTAG 5 cut(s) 162, 375, 393, 591, 753
MaeII ACGT 2 cut(s) 93, 712
MaeIII GTNAC 1 cut(s) 586
MalI GATC 6 cut(s) 115, 337, 512, 557, 576, 736
MboI GATC 6 cut(s) 113, 335, 510, 555, 574, 734
MboII GAAGA 4 cut(s) 268, 394, 408, 680
MflI RGATCY 2 cut(s) 335, 734
MhlI GDGCHC 1 cut(s) 704
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 2 cut(s) 343, 410
MluNI TGGCCA 1 cut(s) 356
MmeI TCCRAC 2 cut(s) 233, 433
Mox20I TGGCCA 1 cut(s) 356
MroNI GCCGGC 2 cut(s) 42, 646
MscI TGGCCA 1 cut(s) 356
MseI TTAA 3 cut(s) 126, 201, 507
MslI CAYNNNNRTG 2 cut(s) 26, 683
Msp20I TGGCCA 1 cut(s) 356
MspA1I CMGCKG 2 cut(s) 109, 238
MspI CCGG 2 cut(s) 43, 647
MspR9I CCNGG 2 cut(s) 744, 799
Mva1269I GAATGC 1 cut(s) 766
MvaI CCWGG 2 cut(s) 744, 799
MvnI CGCG 2 cut(s) 109, 434
MwoI GCNNNNNNNGC 4 cut(s) 103, 187, 362, 467
NaeI GCCGGC 2 cut(s) 44, 648
NcoI CCATGG 1 cut(s) 438
NdeII GATC 6 cut(s) 113, 335, 510, 555, 574, 734
NgoMIV GCCGGC 2 cut(s) 42, 646
NlaIII CATG 2 cut(s) 442, 544
NmuCI GTSAC 1 cut(s) 586
NspI RCATGY 1 cut(s) 544
NspV TTCGAA 1 cut(s) 405
OliI CACNNNNGTG 2 cut(s) 26, 683
PaeI GCATGC 1 cut(s) 544
PaqCI CACCTGC 1 cut(s) 461
PctI GAATGC 1 cut(s) 766
PdiI GCCGGC 2 cut(s) 44, 648
PfeI GAWTC 1 cut(s) 631
PkrI GCNGC 6 cut(s) 108, 145, 237, 295, 677, 725
Ppu21I YACGTR 1 cut(s) 713
Psp6I CCWGG 2 cut(s) 742, 797
PspGI CCWGG 2 cut(s) 742, 797
PspPI GGNCC 4 cut(s) 8, 644, 740, 795
PsuI RGATCY 2 cut(s) 335, 734
RigI GGCCGGCC 1 cut(s) 650
RsaI GTAC 1 cut(s) 711
RsaNI GTAC 1 cut(s) 710
RseI CAYNNNNRTG 2 cut(s) 26, 683
SacII CCGCGG 1 cut(s) 110
SaqAI TTAA 3 cut(s) 126, 201, 507
SatI GCNGC 6 cut(s) 107, 144, 236, 294, 676, 724
Sau3AI GATC 6 cut(s) 113, 335, 510, 555, 574, 734
Sau96I GGNCC 4 cut(s) 8, 644, 740, 795
ScrFI CCNGG 2 cut(s) 744, 799
SduI GDGCHC 1 cut(s) 704
SfaNI GCATC 1 cut(s) 575
Sfr303I CCGCGG 1 cut(s) 110
SfuI TTCGAA 1 cut(s) 405
SgrBI CCGCGG 1 cut(s) 110
SinI GGWCC 1 cut(s) 8
SmiMI CAYNNNNRTG 2 cut(s) 26, 683
SpeI ACTAGT 1 cut(s) 590
SphI GCATGC 1 cut(s) 544
Sse9I AATT 2 cut(s) 343, 410
SsiI CCGC 9 cut(s) 46, 104, 107, 109, 143, 224, 238, 705, 723
SspMI CTAG 5 cut(s) 162, 375, 393, 591, 753
StyD4I CCNGG 2 cut(s) 742, 797
StyI CCWWGG 2 cut(s) 438, 545
TaaI ACNGT 2 cut(s) 8, 28
TaiI ACGT 2 cut(s) 96, 715
TaqI TCGA 4 cut(s) 16, 116, 405, 634
TasI AATT 2 cut(s) 343, 410
TauI GCSGC 3 cut(s) 109, 146, 726
TfiI GAWTC 1 cut(s) 631
Tru1I TTAA 3 cut(s) 126, 201, 507
Tru9I TTAA 3 cut(s) 126, 201, 507
TscAI CASTG 2 cut(s) 33, 520
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 3 cut(s) 235, 293, 675
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 4 cut(s) 17, 87, 186, 275
TspGWI ACGGA 1 cut(s) 411
TspRI CASTG 2 cut(s) 33, 520
VpaK11BI GGWCC 1 cut(s) 8
XapI RAATTY 1 cut(s) 410
XbaI TCTAGA 1 cut(s) 752
XceI RCATGY 1 cut(s) 544
XspI CTAG 5 cut(s) 162, 375, 393, 591, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.