RLG00000021609

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0019861
Physical Location & Seq
Reverse (-)
76709826 .. 76711133
1308 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021609

Sequence Viewer

Length: 531 bp
ATGTCGGAGATGACGACGACATCGGTGTTGGAAAACGACGGGGAAGAAGCATGTCGATTTCTGGGACTTGCTTCGGAGTTCCACGCGTCATCTTCGTCAACTGAGAGCCCATCGTTATCGACGAGGTCCGCATCAGAACTTCGCCCCGAGCAGCTCATTTCCGACAAGGAGGACGCCGCTAACAACTTCGCTCGTGGCCATACACCACCGGCAAAGAGATTATCGATCTCTGCTTGGATCGGATCCGGAAGCTCGACGACAACTGCACTGGGCTTCAGGGATTTCTGGTCTTCAATGTCGTCAGTGGTAATACCGGGTCGGGTCTCGAGTCGCTTCTGCTGGAGGCTGGAGCGTCTTTCTGTGGACTATGGGAAAACGTCCGAGCTCGGATTCACTGTGTATCCTTCTCCTCAGGTTTCTACGTCTGTTGTAGAGCCCTGTAACAGTGTCCTCTCCACTCATTCTCTGCTTGAGCACACCGATGCGTCTGTTTTGCTTGATAATGAGGCCATCTACGACATCTGCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

177

Amino Acids

19.12

Weight (kDa)

4.94

Isoelectric Point (pI)

51.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 116 - 175 1.9e-09 Tubulin/FtsZ family, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019861)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0344731 RchiOBHm_Chr2g0112001 RchiOBHm_Chr2g0153431
rosa_laevigata RLG00000012506 RLG00000021609 RLG00000035396
rosa_multiflora Rmu_sc0006888.1_g000019

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 86
AccIII TCCGGA 1 cut(s) 245
AciI CCGC 3 cut(s) 129, 177, 526
AclWI GGATC 3 cut(s) 237, 245, 250
AcoI YGGCCR 1 cut(s) 196
AcuI CTGAAG 1 cut(s) 259
AcyI GRCGYC 1 cut(s) 174
AflIII ACRYGT 1 cut(s) 84
AgsI TTSAA 1 cut(s) 294
AluBI AGCT 3 cut(s) 154, 252, 385
AluI AGCT 3 cut(s) 154, 252, 385
Alw21I GWGCWC 2 cut(s) 387, 477
Alw26I GTCTC 1 cut(s) 328
AlwI GGATC 3 cut(s) 237, 245, 250
Ama87I CYCGRG 2 cut(s) 146, 325
Aor13HI TCCGGA 1 cut(s) 245
AoxI GGCC 2 cut(s) 196, 507
ApeKI GCWGC 1 cut(s) 151
AspS9I GGNCC 1 cut(s) 126
AsuC2I CCSGG 1 cut(s) 315
AvaI CYCGRG 2 cut(s) 146, 325
AvaII GGWCC 1 cut(s) 126
AxyI CCTNAGG 1 cut(s) 411
BalI TGGCCA 1 cut(s) 198
BamHI GGATCC 1 cut(s) 242
BanII GRGCYC 3 cut(s) 110, 387, 438
BauI CACGAG 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 282
Bbv12I GWGCWC 2 cut(s) 387, 477
BbvI GCAGC 1 cut(s) 163
BccI CCATC 2 cut(s) 118, 518
BciVI GTATCC 1 cut(s) 411
BcnI CCSGG 1 cut(s) 315
BcoDI GTCTC 1 cut(s) 328
BfuI GTATCC 1 cut(s) 411
BisI GCNGC 3 cut(s) 152, 177, 526
BlsI GCNGC 3 cut(s) 153, 178, 527
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 126
BmeT110I CYCGRG 2 cut(s) 146, 325
BmgT120I GGNCC 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 244
BmrFI CCNGG 1 cut(s) 315
BmrI ACTGGG 1 cut(s) 278
BmsI GCATC 2 cut(s) 140, 472
BmuI ACTGGG 1 cut(s) 278
BpiI GAAGAC 1 cut(s) 282
BpmI CTGGAG 2 cut(s) 361, 368
BpuEI CTTGAG 1 cut(s) 491
BpuMI CCSGG 1 cut(s) 315
Bsa29I ATCGAT 1 cut(s) 224
BsaHI GRCGYC 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 328
BsaWI WCCGGW 1 cut(s) 245
Bse118I RCCGGY 1 cut(s) 208
Bse1I ACTGG 1 cut(s) 273
Bse21I CCTNAGG 1 cut(s) 411
BseAI TCCGGA 1 cut(s) 245
BseCI ATCGAT 1 cut(s) 224
BseMII CTCAG 2 cut(s) 93, 425
BseNI ACTGG 1 cut(s) 273
BseRI GAGGAG 1 cut(s) 399
BseXI GCAGC 1 cut(s) 163
BsgI GTGCAG 1 cut(s) 249
Bsh1236I CGCG 1 cut(s) 86
BshFI GGCC 2 cut(s) 198, 509
BshVI ATCGAT 1 cut(s) 224
BsiHKAI GWGCWC 2 cut(s) 387, 477
BsiHKCI CYCGRG 2 cut(s) 146, 325
BsiSI CCGG 3 cut(s) 209, 246, 314
BslFI GGGAC 1 cut(s) 78
BsmAI GTCTC 1 cut(s) 328
BsmFI GGGAC 1 cut(s) 78
BsnI GGCC 2 cut(s) 198, 509
Bso31I GGTCTC 1 cut(s) 328
BsoBI CYCGRG 2 cut(s) 146, 325
Bsp1286I GDGCHC 4 cut(s) 110, 387, 438, 477
Bsp13I TCCGGA 1 cut(s) 245
Bsp143I GATC 3 cut(s) 225, 237, 242
BspACI CCGC 3 cut(s) 129, 177, 526
BspANI GGCC 2 cut(s) 198, 509
BspCNI CTCAG 2 cut(s) 94, 424
BspDI ATCGAT 1 cut(s) 224
BspEI TCCGGA 1 cut(s) 245
BspFNI CGCG 1 cut(s) 86
BspLI GGNNCC 1 cut(s) 244
BspPI GGATC 3 cut(s) 237, 245, 250
BspTNI GGTCTC 1 cut(s) 328
BsrFI RCCGGY 1 cut(s) 208
BsrI ACTGG 1 cut(s) 273
BssAI RCCGGY 1 cut(s) 208
BssMI GATC 3 cut(s) 225, 237, 242
BssNI GRCGYC 1 cut(s) 174
BssSI CACGAG 1 cut(s) 192
Bst2BI CACGAG 1 cut(s) 192
Bst4CI ACNGT 2 cut(s) 397, 446
BstACI GRCGYC 1 cut(s) 174
BstDEI CTNAG 2 cut(s) 102, 411
BstFNI CGCG 1 cut(s) 86
BstKTI GATC 3 cut(s) 228, 240, 245
BstMAI GTCTC 1 cut(s) 328
BstMBI GATC 3 cut(s) 225, 237, 242
BstNSI RCATGY 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 313
BstUI CGCG 1 cut(s) 86
BstV1I GCAGC 1 cut(s) 163
BstV2I GAAGAC 1 cut(s) 282
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
Bsu15I ATCGAT 1 cut(s) 224
Bsu36I CCTNAGG 1 cut(s) 411
BsuI GTATCC 1 cut(s) 411
BsuRI GGCC 2 cut(s) 198, 509
BsuTUI ATCGAT 1 cut(s) 224
BtsIMutI CAGTG 4 cut(s) 266, 309, 393, 451
Cfr10I RCCGGY 1 cut(s) 208
Cfr13I GGNCC 1 cut(s) 126
ClaI ATCGAT 1 cut(s) 224
CseI GACGC 4 cut(s) 75, 182, 341, 474
CviAII CATG 1 cut(s) 51
CviJI RGCY 9 cut(s) 108, 154, 198, 252, 273, 346, 385, 436, 509
CviKI_1 RGCY 9 cut(s) 108, 154, 198, 252, 273, 346, 385, 436, 509
DdeI CTNAG 2 cut(s) 102, 411
DpnI GATC 3 cut(s) 227, 239, 244
DpnII GATC 3 cut(s) 225, 237, 242
EaeI YGGCCR 1 cut(s) 196
Ecl136II GAGCTC 1 cut(s) 385
Eco24I GRGCYC 3 cut(s) 110, 387, 438
Eco31I GGTCTC 1 cut(s) 328
Eco47I GGWCC 1 cut(s) 126
Eco53kI GAGCTC 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 259
Eco81I CCTNAGG 1 cut(s) 411
Eco88I CYCGRG 2 cut(s) 146, 325
EcoICRI GAGCTC 1 cut(s) 385
EcoT38I GRGCYC 3 cut(s) 110, 387, 438
FaeI CATG 1 cut(s) 54
FaiI YATR 3 cut(s) 52, 201, 369
FaqI GGGAC 1 cut(s) 78
FatI CATG 1 cut(s) 50
Fnu4HI GCNGC 3 cut(s) 152, 177, 526
FriOI GRGCYC 3 cut(s) 110, 387, 438
Fsp4HI GCNGC 3 cut(s) 152, 177, 526
GluI GCNGC 3 cut(s) 152, 177, 526
GsuI CTGGAG 2 cut(s) 361, 368
HaeIII GGCC 2 cut(s) 198, 509
HapII CCGG 3 cut(s) 209, 246, 314
HgaI GACGC 4 cut(s) 75, 182, 341, 474
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 1 cut(s) 54
HincII GTYRAC 1 cut(s) 99
HindII GTYRAC 1 cut(s) 99
HinfI GANTC 2 cut(s) 328, 390
HpaII CCGG 3 cut(s) 209, 246, 314
Hpy166II GTNNAC 2 cut(s) 99, 364
Hpy188I TCNGA 7 cut(s) 7, 76, 136, 163, 242, 382, 389
Hpy188III TCNNGA 2 cut(s) 246, 325
Hpy8I GTNNAC 2 cut(s) 99, 364
Hpy99I CGWCG 4 cut(s) 19, 41, 124, 259
HpyAV CCTTC 1 cut(s) 414
HpyCH4III ACNGT 2 cut(s) 397, 446
HpyCH4IV ACGT 2 cut(s) 377, 422
HpyCH4V TGCA 1 cut(s) 266
HpyF3I CTNAG 2 cut(s) 102, 411
HpySE526I ACGT 2 cut(s) 377, 422
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 1 cut(s) 54
Kpn2I TCCGGA 1 cut(s) 245
Kzo9I GATC 3 cut(s) 225, 237, 242
LmnI GCTCC 1 cut(s) 349
Lsp1109I GCAGC 1 cut(s) 163
LweI GCATC 2 cut(s) 140, 472
MaeII ACGT 2 cut(s) 377, 422
MaeIII GTNAC 1 cut(s) 440
MalI GATC 3 cut(s) 227, 239, 244
MboI GATC 3 cut(s) 225, 237, 242
MboII GAAGA 3 cut(s) 56, 84, 282
MflI RGATCY 1 cut(s) 242
MhlI GDGCHC 4 cut(s) 110, 387, 438, 477
MlsI TGGCCA 1 cut(s) 198
MluI ACGCGT 1 cut(s) 84
MluNI TGGCCA 1 cut(s) 198
MlyI GAGTC 1 cut(s) 337
MmeI TCCRAC 2 cut(s) 9, 186
MnlI CCTC 6 cut(s) 117, 163, 336, 420, 461, 499
Mox20I TGGCCA 1 cut(s) 198
MroI TCCGGA 1 cut(s) 245
MscI TGGCCA 1 cut(s) 198
MslI CAYNNNNRTG 1 cut(s) 480
Msp20I TGGCCA 1 cut(s) 198
MspA1I CMGCKG 1 cut(s) 528
MspI CCGG 3 cut(s) 209, 246, 314
MspR9I CCNGG 1 cut(s) 315
MvnI CGCG 1 cut(s) 86
NciI CCSGG 1 cut(s) 315
NdeII GATC 3 cut(s) 225, 237, 242
NlaIII CATG 1 cut(s) 54
NlaIV GGNNCC 1 cut(s) 244
NspI RCATGY 1 cut(s) 54
PaeR7I CTCGAG 1 cut(s) 325
PcsI WCGNNNNNNNCGW 2 cut(s) 11, 119
PfeI GAWTC 1 cut(s) 390
PflFI GACNNNGTC 1 cut(s) 124
PkrI GCNGC 3 cut(s) 153, 178, 527
PleI GAGTC 1 cut(s) 336
PpsI GAGTC 1 cut(s) 336
Psp124BI GAGCTC 1 cut(s) 387
PspN4I GGNNCC 1 cut(s) 244
PspPI GGNCC 1 cut(s) 126
PsuI RGATCY 1 cut(s) 242
PsyI GACNNNGTC 1 cut(s) 124
RseI CAYNNNNRTG 1 cut(s) 480
SacI GAGCTC 1 cut(s) 387
SatI GCNGC 3 cut(s) 152, 177, 526
Sau3AI GATC 3 cut(s) 225, 237, 242
Sau96I GGNCC 1 cut(s) 126
SchI GAGTC 1 cut(s) 337
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 4 cut(s) 110, 387, 438, 477
SetI ASST 7 cut(s) 128, 156, 254, 380, 387, 417, 425
SfaNI GCATC 2 cut(s) 140, 472
Sfr274I CTCGAG 1 cut(s) 325
SinI GGWCC 1 cut(s) 126
SlaI CTCGAG 1 cut(s) 325
SmiMI CAYNNNNRTG 1 cut(s) 480
SmlI CTYRAG 2 cut(s) 325, 470
SmoI CTYRAG 2 cut(s) 325, 470
SsiI CCGC 3 cut(s) 129, 177, 526
SstI GAGCTC 1 cut(s) 387
StyD4I CCNGG 1 cut(s) 313
TaaI ACNGT 2 cut(s) 397, 446
TaiI ACGT 2 cut(s) 380, 425
TaqI TCGA 5 cut(s) 55, 119, 224, 254, 326
TauI GCSGC 2 cut(s) 179, 528
TfiI GAWTC 1 cut(s) 390
TscAI CASTG 4 cut(s) 273, 309, 400, 451
TseI GCWGC 1 cut(s) 151
TspRI CASTG 4 cut(s) 273, 309, 400, 451
Tth111I GACNNNGTC 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 126
XceI RCATGY 1 cut(s) 54
XhoI CTCGAG 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.