RLG00000035396

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0019861
Physical Location & Seq
Reverse (-)
66279329 .. 66281023
1695 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035396

Sequence Viewer

Length: 483 bp
ATGGTCCGTTCTACAGGGGTGGAGGCCAGTGGTAGGCGGCTTGGCGGCTTCGTTATAAACCAAGATGCGCTTCCAATGCCCAATGGAGGAAGGCGAGAGGCTTGGTGGTTGCATGTGCCGGAGGACGCCGCTAACAACTTCGCCCGTGGCCATACACCACCGGCAAAGAGATCGTTGATCTCTGCTTGGATCGGATCTGGAAGCTCGACGACAACTGCACTGGGCTTCAGAGATTTCTGGTCATCAATGTCGTCGGTGGTGATACCGGGTCGGGTCTCGGGTCGCTTCTGCTGGAGGCTGGAGCGTCTTTCTGTGGACTATGGGAAAACGTCCGAGCTCGGATTCACTGTGTATCCTTCTCCTCAGGTTTCTACGTCTGTTGTAGAGCCCTGTAACAGTGTCCTCTCTACTCATTCTCTGCTTAAGCACACCGATGCGTCTGTTTTGCTTGATAATGAGGCCATCTACGACATCTGCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

161

Amino Acids

17.47

Weight (kDa)

8.57

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 100 - 159 1.5e-09 Tubulin/FtsZ family, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019861)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0344731 RchiOBHm_Chr2g0112001 RchiOBHm_Chr2g0153431
rosa_laevigata RLG00000012506 RLG00000021609 RLG00000035396
rosa_multiflora Rmu_sc0006888.1_g000019

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 56
AciI CCGC 4 cut(s) 37, 45, 129, 478
AclWI GGATC 2 cut(s) 197, 202
AcoI YGGCCR 1 cut(s) 148
AcuI CTGAAG 1 cut(s) 211
AcyI GRCGYC 1 cut(s) 126
AfiI CCNNNNNNNGG 2 cut(s) 33, 86
AflII CTTAAG 1 cut(s) 422
AjuI GAANNNNNNNTTGG 2 cut(s) 54, 86
AluBI AGCT 2 cut(s) 204, 337
AluI AGCT 2 cut(s) 204, 337
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 2 cut(s) 197, 202
Ama87I CYCGRG 1 cut(s) 277
AoxI GGCC 3 cut(s) 24, 148, 459
AspLEI GCGC 1 cut(s) 70
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 267
AsuHPI GGTGA 1 cut(s) 271
AvaI CYCGRG 1 cut(s) 277
AvaII GGWCC 1 cut(s) 4
AxyI CCTNAGG 1 cut(s) 363
BalI TGGCCA 1 cut(s) 150
BanII GRGCYC 2 cut(s) 339, 390
Bbv12I GWGCWC 1 cut(s) 339
BccI CCATC 1 cut(s) 470
BcgI CGANNNNNNTGC 2 cut(s) 153, 187
BciVI GTATCC 1 cut(s) 363
BcnI CCSGG 1 cut(s) 267
BcoDI GTCTC 1 cut(s) 280
BfmI CTRYAG 1 cut(s) 12
BfrI CTTAAG 1 cut(s) 422
BfuI GTATCC 1 cut(s) 363
BisI GCNGC 4 cut(s) 38, 46, 129, 478
BlsI GCNGC 4 cut(s) 39, 47, 130, 479
Bme1390I CCNGG 1 cut(s) 267
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 1 cut(s) 277
BmgT120I GGNCC 1 cut(s) 4
BmrFI CCNGG 1 cut(s) 267
BmrI ACTGGG 1 cut(s) 230
BmsI GCATC 2 cut(s) 55, 424
BmuI ACTGGG 1 cut(s) 230
BpmI CTGGAG 2 cut(s) 313, 320
BpuMI CCSGG 1 cut(s) 267
BsaHI GRCGYC 1 cut(s) 126
BsaI GGTCTC 1 cut(s) 280
BsaJI CCNNGG 1 cut(s) 145
Bsc4I CCNNNNNNNGG 2 cut(s) 33, 86
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 2 cut(s) 27, 225
Bse21I CCTNAGG 1 cut(s) 363
BseDI CCNNGG 1 cut(s) 145
BseLI CCNNNNNNNGG 2 cut(s) 33, 86
BseMII CTCAG 1 cut(s) 377
BseNI ACTGG 2 cut(s) 27, 225
BseRI GAGGAG 1 cut(s) 351
BsgI GTGCAG 1 cut(s) 201
BshFI GGCC 3 cut(s) 26, 150, 461
BsiHKAI GWGCWC 1 cut(s) 339
BsiHKCI CYCGRG 1 cut(s) 277
BsiSI CCGG 3 cut(s) 119, 161, 266
BslI CCNNNNNNNGG 2 cut(s) 33, 86
BsmAI GTCTC 1 cut(s) 280
BsnI GGCC 3 cut(s) 26, 150, 461
Bso31I GGTCTC 1 cut(s) 280
BsoBI CYCGRG 1 cut(s) 277
Bsp1286I GDGCHC 2 cut(s) 339, 390
Bsp143I GATC 4 cut(s) 170, 177, 189, 194
BspACI CCGC 4 cut(s) 37, 45, 129, 478
BspANI GGCC 3 cut(s) 26, 150, 461
BspCNI CTCAG 1 cut(s) 376
BspPI GGATC 2 cut(s) 197, 202
BspTI CTTAAG 1 cut(s) 422
BspTNI GGTCTC 1 cut(s) 280
BsrFI RCCGGY 1 cut(s) 160
BsrI ACTGG 2 cut(s) 27, 225
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 1 cut(s) 145
BssMI GATC 4 cut(s) 170, 177, 189, 194
BssNI GRCGYC 1 cut(s) 126
Bst4CI ACNGT 2 cut(s) 349, 398
BstACI GRCGYC 1 cut(s) 126
BstAFI CTTAAG 1 cut(s) 422
BstDEI CTNAG 1 cut(s) 363
BstDSI CCRYGG 1 cut(s) 145
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 4 cut(s) 173, 180, 192, 197
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 4 cut(s) 170, 177, 189, 194
BstMWI GCNNNNNNNGC 1 cut(s) 76
BstNSI RCATGY 1 cut(s) 116
BstSCI CCNGG 1 cut(s) 265
BstSFI CTRYAG 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 194
BstYI RGATCY 1 cut(s) 194
Bsu36I CCTNAGG 1 cut(s) 363
BsuI GTATCC 1 cut(s) 363
BsuRI GGCC 3 cut(s) 26, 150, 461
BtgI CCRYGG 1 cut(s) 145
BtsIMutI CAGTG 4 cut(s) 34, 218, 345, 403
CfoI GCGC 1 cut(s) 70
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 3 cut(s) 134, 293, 426
CviAII CATG 1 cut(s) 113
DdeI CTNAG 1 cut(s) 363
DpnI GATC 4 cut(s) 172, 179, 191, 196
DpnII GATC 4 cut(s) 170, 177, 189, 194
EaeI YGGCCR 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 337
Eco24I GRGCYC 2 cut(s) 339, 390
Eco31I GGTCTC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 4
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 1 cut(s) 211
Eco81I CCTNAGG 1 cut(s) 363
Eco88I CYCGRG 1 cut(s) 277
EcoICRI GAGCTC 1 cut(s) 337
EcoT38I GRGCYC 2 cut(s) 339, 390
FaeI CATG 1 cut(s) 116
FaiI YATR 4 cut(s) 56, 114, 153, 321
FalI AAGNNNNNCTT 2 cut(s) 54, 86
FatI CATG 1 cut(s) 112
Fnu4HI GCNGC 4 cut(s) 38, 46, 129, 478
FriOI GRGCYC 2 cut(s) 339, 390
Fsp4HI GCNGC 4 cut(s) 38, 46, 129, 478
GlaI GCGC 1 cut(s) 69
GluI GCNGC 4 cut(s) 38, 46, 129, 478
GsuI CTGGAG 2 cut(s) 313, 320
HaeIII GGCC 3 cut(s) 26, 150, 461
HapII CCGG 3 cut(s) 119, 161, 266
HgaI GACGC 3 cut(s) 134, 293, 426
HhaI GCGC 1 cut(s) 70
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 1 cut(s) 116
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HinfI GANTC 1 cut(s) 342
HpaII CCGG 3 cut(s) 119, 161, 266
HphI GGTGA 1 cut(s) 271
Hpy166II GTNNAC 1 cut(s) 316
Hpy188I TCNGA 4 cut(s) 194, 230, 334, 341
Hpy188III TCNNGA 1 cut(s) 198
Hpy8I GTNNAC 1 cut(s) 316
Hpy99I CGWCG 2 cut(s) 211, 256
HpyAV CCTTC 2 cut(s) 84, 366
HpyCH4III ACNGT 2 cut(s) 349, 398
HpyCH4IV ACGT 2 cut(s) 329, 374
HpyCH4V TGCA 2 cut(s) 112, 218
HpyF10VI GCNNNNNNNGC 1 cut(s) 76
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 2 cut(s) 329, 374
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 1 cut(s) 116
HspAI GCGC 1 cut(s) 68
Kzo9I GATC 4 cut(s) 170, 177, 189, 194
LmnI GCTCC 1 cut(s) 301
LweI GCATC 2 cut(s) 55, 424
MaeII ACGT 2 cut(s) 329, 374
MaeIII GTNAC 1 cut(s) 392
MalI GATC 4 cut(s) 172, 179, 191, 196
MboI GATC 4 cut(s) 170, 177, 189, 194
MflI RGATCY 1 cut(s) 194
MhlI GDGCHC 2 cut(s) 339, 390
MlsI TGGCCA 1 cut(s) 150
MluNI TGGCCA 1 cut(s) 150
MnlI CCTC 8 cut(s) 16, 80, 91, 115, 288, 372, 413, 451
Mox20I TGGCCA 1 cut(s) 150
MscI TGGCCA 1 cut(s) 150
MseI TTAA 1 cut(s) 423
MslI CAYNNNNRTG 1 cut(s) 432
Msp20I TGGCCA 1 cut(s) 150
MspA1I CMGCKG 1 cut(s) 480
MspCI CTTAAG 1 cut(s) 422
MspI CCGG 3 cut(s) 119, 161, 266
MspR9I CCNGG 1 cut(s) 267
MwoI GCNNNNNNNGC 1 cut(s) 76
NciI CCSGG 1 cut(s) 267
NdeII GATC 4 cut(s) 170, 177, 189, 194
NlaIII CATG 1 cut(s) 116
NspI RCATGY 1 cut(s) 116
PfeI GAWTC 1 cut(s) 342
PkrI GCNGC 4 cut(s) 39, 47, 130, 479
PsiI TTATAA 1 cut(s) 56
Psp124BI GAGCTC 1 cut(s) 339
PspPI GGNCC 1 cut(s) 4
PsuI RGATCY 1 cut(s) 194
RseI CAYNNNNRTG 1 cut(s) 432
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 4 cut(s) 38, 46, 129, 478
Sau3AI GATC 4 cut(s) 170, 177, 189, 194
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 1 cut(s) 267
SduI GDGCHC 2 cut(s) 339, 390
SetI ASST 5 cut(s) 206, 332, 339, 369, 377
SfaNI GCATC 2 cut(s) 55, 424
SfcI CTRYAG 1 cut(s) 12
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SsiI CCGC 4 cut(s) 37, 45, 129, 478
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 1 cut(s) 265
TaaI ACNGT 2 cut(s) 349, 398
TaiI ACGT 2 cut(s) 332, 377
TaqI TCGA 1 cut(s) 206
TauI GCSGC 4 cut(s) 40, 48, 131, 480
TfiI GAWTC 1 cut(s) 342
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 4 cut(s) 34, 225, 352, 403
TspRI CASTG 4 cut(s) 34, 225, 352, 403
Vha464I CTTAAG 1 cut(s) 422
VpaK11BI GGWCC 1 cut(s) 4
XceI RCATGY 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.