RLG00000021754

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0020672
Physical Location & Seq
Reverse (-)
77985348 .. 77989128
3781 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021754

Sequence Viewer

Length: 927 bp
ATGCATCAGACCATGGCAGCAGATAAGCACAGGGCAAGAACTGTTGTTGTTCCATTGGAAAGACTGAACCTACTATTGAAATTCAATTTGTGGCAATCTGTAATATTTGATGTTCATGAGGGTGGATACACTCTTGACTTTCCTACAACCTTGCATAGGAGTAAAGCCGACTGTCCACAGCCTGAGGAAATGGCCACTGTCAGTTTTGATGGTGCTGTACTTGGACAAATTCCTGCAATTGTTGCATATCTTTCAGACAAAGGAATCTTTATTGTCGAAGATAAAGTAGGAGGAGTTCAACTGTGGGATAGTTTCGGTCTCCCTATCAATACAATCTTACCTGAACAAATGTTAGGATTTGATAGCAAATCTGGAACGAAGAATGTCATGACTTCCTGGAAAAGTGAGAATGATCCAACATCTGGGAGATATTTGGTTGGATTGACACCTGAGACAACAACACGAGTGTTCTTTTGGATCAATGGATCAATTCCCCACTGGAGGATTGGGCCTTGGGATAAATCAAAGTTCATTGAAATATCAGAGATGGATGATCGATATGGAAGTGAATTTAGTCTGAAGGAAGATGAAACAGAGGGGTCAGGGTATTCATCTTATTCTTTTTGTGAGGGTAGACTCACCTATTTACACATTCAGAAAGAACATCAAAGCTTATGCTGTCAGATAGTGGTAAGAACTGGTATGCAAACTTCACGGGACCAAAACTCCCATGCGACAGTTATGGAGCATGCGGACCTTTTGGTGTTTGCAAGGATTGAATCTCCATTATGTGTTAACAACCTTCACATCACAGGCATTGACAACTTCCAGATTGCAAAGTTCTTAAGCATTTCTTCGTCGGAGTTGGATACTTGGATTGAAGGTTGTAGAGGGGTTGCAGCCGTTGGACTCATCGAGAACAATTGA

Protein Analysis

309

Amino Acids

34.56

Weight (kDa)

5.2

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RED_N PF07808 35 - 86 2.8e-10 RED-like protein N-terminal region
B_lectin PF01453 80 - 134 1e-08 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020672)

Species Orthologous Gene IDs
rosa_laevigata RLG00000021754 RLG00000036711
rosa_multiflora Rmu_sc0004168.1_g000002
rosa_roxburghii Rroxscaffold_1G00004360
rosa_rugosa Rorug05G0456400
rosa_samantha Rh5CG556500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 422
AccI GTMKAC 1 cut(s) 634
AciI CCGC 1 cut(s) 752
AclWI GGATC 3 cut(s) 407, 485, 493
AcoI YGGCCR 1 cut(s) 192
AcsI RAATTY 3 cut(s) 80, 228, 569
AcuI CTGAAG 1 cut(s) 599
AfaI GTAC 1 cut(s) 219
AfiI CCNNNNNNNGG 3 cut(s) 156, 422, 501
AflII CTTAAG 1 cut(s) 844
AgsI TTSAA 6 cut(s) 79, 85, 299, 536, 779, 881
AjnI CCWGG 1 cut(s) 395
AluBI AGCT 1 cut(s) 672
AluI AGCT 1 cut(s) 672
Alw26I GTCTC 2 cut(s) 323, 446
AlwI GGATC 3 cut(s) 407, 485, 493
AoxI GGCC 2 cut(s) 192, 509
ApeKI GCWGC 2 cut(s) 17, 899
ApoI RAATTY 3 cut(s) 80, 228, 569
AspS9I GGNCC 3 cut(s) 509, 718, 754
AsuHPI GGTGA 1 cut(s) 631
AvaII GGWCC 2 cut(s) 718, 754
AxyI CCTNAGG 1 cut(s) 183
BalI TGGCCA 1 cut(s) 194
BarI GAAGNNNNNNTAC 2 cut(s) 694, 726
BauI CACGAG 1 cut(s) 462
BbvI GCAGC 2 cut(s) 29, 911
BccI CCATC 2 cut(s) 203, 541
BceAI ACGGC 1 cut(s) 887
BciT130I CCWGG 1 cut(s) 397
BciVI GTATCC 2 cut(s) 119, 862
BcoDI GTCTC 2 cut(s) 323, 446
BfrI CTTAAG 1 cut(s) 844
BfuI GTATCC 2 cut(s) 119, 862
BisI GCNGC 2 cut(s) 18, 900
BlsI GCNGC 2 cut(s) 19, 901
Bme1390I CCNGG 1 cut(s) 397
Bme18I GGWCC 2 cut(s) 718, 754
BmgT120I GGNCC 3 cut(s) 509, 718, 754
BmiI GGNNCC 1 cut(s) 719
BmrFI CCNGG 1 cut(s) 397
BmsI GCATC 1 cut(s) 13
BpmI CTGGAG 1 cut(s) 520
Bsa29I ATCGAT 1 cut(s) 556
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 2 cut(s) 12, 512
BsaXI ACNNNNNCTCC 2 cut(s) 710, 740
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 422, 501
Bse1I ACTGG 2 cut(s) 503, 703
Bse21I CCTNAGG 1 cut(s) 183
BseBI CCWGG 1 cut(s) 397
BseCI ATCGAT 1 cut(s) 556
BseDI CCNNGG 2 cut(s) 12, 512
BseGI GGATG 1 cut(s) 556
BseLI CCNNNNNNNGG 3 cut(s) 156, 422, 501
BseMII CTCAG 2 cut(s) 174, 441
BseNI ACTGG 2 cut(s) 503, 703
BseRI GAGGAG 1 cut(s) 306
BseXI GCAGC 2 cut(s) 29, 911
BshFI GGCC 2 cut(s) 194, 511
BshVI ATCGAT 1 cut(s) 556
BslFI GGGAC 1 cut(s) 731
BslI CCNNNNNNNGG 3 cut(s) 156, 422, 501
BsmAI GTCTC 2 cut(s) 323, 446
BsmFI GGGAC 1 cut(s) 731
BsnI GGCC 2 cut(s) 194, 511
Bso31I GGTCTC 1 cut(s) 323
Bsp143I GATC 4 cut(s) 412, 477, 485, 553
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 1 cut(s) 752
BspANI GGCC 2 cut(s) 194, 511
BspCNI CTCAG 2 cut(s) 175, 442
BspDI ATCGAT 1 cut(s) 556
BspHI TCATGA 2 cut(s) 115, 387
BspLI GGNNCC 1 cut(s) 719
BspPI GGATC 3 cut(s) 407, 485, 493
BspTI CTTAAG 1 cut(s) 844
BspTNI GGTCTC 1 cut(s) 323
BsrI ACTGG 2 cut(s) 503, 703
BssECI CCNNGG 2 cut(s) 12, 512
BssMI GATC 4 cut(s) 412, 477, 485, 553
BssSI CACGAG 1 cut(s) 462
BssT1I CCWWGG 2 cut(s) 12, 512
Bst2BI CACGAG 1 cut(s) 462
Bst2UI CCWGG 1 cut(s) 397
Bst4CI ACNGT 5 cut(s) 43, 173, 199, 303, 739
BstAFI CTTAAG 1 cut(s) 844
BstAPI GCANNNNNTGC 1 cut(s) 242
BstC8I GCNNGC 1 cut(s) 750
BstDEI CTNAG 2 cut(s) 183, 450
BstDSI CCRYGG 1 cut(s) 12
BstENI CCTNNNNNAGG 1 cut(s) 154
BstF5I GGATG 1 cut(s) 556
BstKTI GATC 4 cut(s) 415, 480, 488, 556
BstMAI GTCTC 2 cut(s) 323, 446
BstMBI GATC 4 cut(s) 412, 477, 485, 553
BstMWI GCNNNNNNNGC 1 cut(s) 242
BstNI CCWGG 1 cut(s) 397
BstNSI RCATGY 1 cut(s) 752
BstSCI CCNGG 1 cut(s) 395
BstV1I GCAGC 2 cut(s) 29, 911
Bsu15I ATCGAT 1 cut(s) 556
Bsu36I CCTNAGG 1 cut(s) 183
BsuI GTATCC 2 cut(s) 119, 862
BsuRI GGCC 2 cut(s) 194, 511
BsuTUI ATCGAT 1 cut(s) 556
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 1 cut(s) 556
BtsIMutI CAGTG 2 cut(s) 195, 496
Cac8I GCNNGC 1 cut(s) 750
CciI TCATGA 2 cut(s) 115, 387
Cfr13I GGNCC 3 cut(s) 509, 718, 754
ClaI ATCGAT 1 cut(s) 556
Csp6I GTAC 1 cut(s) 218
CviAII CATG 5 cut(s) 13, 116, 388, 731, 749
CviJI RGCY 6 cut(s) 167, 181, 194, 511, 672, 902
CviKI_1 RGCY 6 cut(s) 167, 181, 194, 511, 672, 902
CviQI GTAC 1 cut(s) 218
DdeI CTNAG 2 cut(s) 183, 450
DpnI GATC 4 cut(s) 414, 479, 487, 555
DpnII GATC 4 cut(s) 412, 477, 485, 553
EaeI YGGCCR 1 cut(s) 192
Eco130I CCWWGG 2 cut(s) 12, 512
Eco31I GGTCTC 1 cut(s) 323
Eco47I GGWCC 2 cut(s) 718, 754
Eco57I CTGAAG 1 cut(s) 599
Eco81I CCTNAGG 1 cut(s) 183
EcoNI CCTNNNNNAGG 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 395
EcoT14I CCWWGG 2 cut(s) 12, 512
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 12, 512
FaeI CATG 5 cut(s) 16, 119, 391, 734, 752
FalI AAGNNNNNCTT 2 cut(s) 838, 870
FaqI GGGAC 1 cut(s) 731
FatI CATG 5 cut(s) 12, 115, 387, 730, 748
FblI GTMKAC 1 cut(s) 634
Fnu4HI GCNGC 2 cut(s) 18, 900
FokI GGATG 1 cut(s) 563
Fsp4HI GCNGC 2 cut(s) 18, 900
GluI GCNGC 2 cut(s) 18, 900
GsuI CTGGAG 1 cut(s) 520
HaeIII GGCC 2 cut(s) 194, 511
Hin1II CATG 5 cut(s) 16, 119, 391, 734, 752
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HindIII AAGCTT 1 cut(s) 670
HinfI GANTC 4 cut(s) 264, 636, 779, 909
HpaI GTTAAC 1 cut(s) 796
HphI GGTGA 1 cut(s) 631
Hpy166II GTNNAC 3 cut(s) 176, 635, 796
Hpy188I TCNGA 7 cut(s) 9, 256, 544, 579, 657, 684, 862
Hpy188III TCNNGA 6 cut(s) 116, 134, 372, 388, 829, 916
Hpy8I GTNNAC 3 cut(s) 176, 635, 796
Hpy99I CGWCG 1 cut(s) 862
HpyAV CCTTC 3 cut(s) 574, 812, 875
HpyCH4III ACNGT 5 cut(s) 43, 173, 199, 303, 739
HpyCH4V TGCA 8 cut(s) 4, 154, 236, 245, 706, 770, 836, 899
HpyF10VI GCNNNNNNNGC 1 cut(s) 242
HpyF3I CTNAG 2 cut(s) 183, 450
Hsp92II CATG 5 cut(s) 16, 119, 391, 734, 752
KspAI GTTAAC 1 cut(s) 796
Kzo9I GATC 4 cut(s) 412, 477, 485, 553
LmnI GCTCC 1 cut(s) 745
Lsp1109I GCAGC 2 cut(s) 29, 911
LweI GCATC 1 cut(s) 13
MalI GATC 4 cut(s) 414, 479, 487, 555
MboI GATC 4 cut(s) 412, 477, 485, 553
MboII GAAGA 4 cut(s) 290, 391, 596, 846
MfeI CAATTG 2 cut(s) 237, 922
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 7 cut(s) 80, 85, 228, 237, 489, 569, 922
MluNI TGGCCA 1 cut(s) 194
MlyI GAGTC 2 cut(s) 630, 903
MmeI TCCRAC 5 cut(s) 418, 440, 840, 846, 886
MnlI CCTC 7 cut(s) 112, 178, 284, 495, 589, 622, 884
Mox20I TGGCCA 1 cut(s) 194
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 194
MseI TTAA 2 cut(s) 795, 845
MslI CAYNNNNRTG 1 cut(s) 120
Msp20I TGGCCA 1 cut(s) 194
MspCI CTTAAG 1 cut(s) 844
MspR9I CCNGG 1 cut(s) 397
MunI CAATTG 2 cut(s) 237, 922
MvaI CCWGG 1 cut(s) 397
MwoI GCNNNNNNNGC 1 cut(s) 242
NcoI CCATGG 1 cut(s) 12
NdeII GATC 4 cut(s) 412, 477, 485, 553
NlaIII CATG 5 cut(s) 16, 119, 391, 734, 752
NlaIV GGNNCC 1 cut(s) 719
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 752
PaeI GCATGC 1 cut(s) 752
PagI TCATGA 2 cut(s) 115, 387
PfeI GAWTC 2 cut(s) 264, 779
PflMI CCANNNNNTGG 1 cut(s) 422
PfoI TCCNGGA 1 cut(s) 395
PkrI GCNGC 2 cut(s) 19, 901
PleI GAGTC 2 cut(s) 630, 903
PpsI GAGTC 2 cut(s) 630, 903
Psp6I CCWGG 1 cut(s) 395
PspGI CCWGG 1 cut(s) 395
PspN4I GGNNCC 1 cut(s) 719
PspPI GGNCC 3 cut(s) 509, 718, 754
PsrI GAACNNNNNNTAC 2 cut(s) 279, 311
RsaI GTAC 1 cut(s) 219
RsaNI GTAC 1 cut(s) 218
RseI CAYNNNNRTG 1 cut(s) 120
SaqAI TTAA 2 cut(s) 795, 845
SatI GCNGC 2 cut(s) 18, 900
Sau3AI GATC 4 cut(s) 412, 477, 485, 553
Sau96I GGNCC 3 cut(s) 509, 718, 754
SchI GAGTC 2 cut(s) 630, 903
ScrFI CCNGG 1 cut(s) 397
SetI ASST 9 cut(s) 72, 152, 343, 451, 644, 674, 759, 804, 886
SfaNI GCATC 1 cut(s) 13
SinI GGWCC 2 cut(s) 718, 754
SmiMI CAYNNNNRTG 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 844
SmoI CTYRAG 1 cut(s) 844
SphI GCATGC 1 cut(s) 752
Sse9I AATT 7 cut(s) 80, 85, 228, 237, 489, 569, 922
SsiI CCGC 1 cut(s) 752
SspI AATATT 1 cut(s) 105
StyD4I CCNGG 1 cut(s) 395
StyI CCWWGG 2 cut(s) 12, 512
TaaI ACNGT 5 cut(s) 43, 173, 199, 303, 739
TaqI TCGA 3 cut(s) 276, 556, 915
TaqII GACCGA 1 cut(s) 305
TasI AATT 7 cut(s) 80, 85, 228, 237, 489, 569, 922
TatI WGTACW 1 cut(s) 217
TfiI GAWTC 2 cut(s) 264, 779
Tru1I TTAA 2 cut(s) 795, 845
Tru9I TTAA 2 cut(s) 795, 845
TscAI CASTG 2 cut(s) 202, 503
TseI GCWGC 2 cut(s) 17, 899
TspDTI ATGAA 4 cut(s) 104, 520, 600, 603
TspRI CASTG 2 cut(s) 202, 503
Van91I CCANNNNNTGG 1 cut(s) 422
Vha464I CTTAAG 1 cut(s) 844
VpaK11BI GGWCC 2 cut(s) 718, 754
XagI CCTNNNNNAGG 1 cut(s) 154
XapI RAATTY 3 cut(s) 80, 228, 569
XceI RCATGY 1 cut(s) 752
XcmI CCANNNNNNNNNTGG 1 cut(s) 503
XmiI GTMKAC 1 cut(s) 634
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.