RLG00000021861

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018067
Physical Location & Seq
Reverse (-)
78886128 .. 78887836
1709 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021861

Sequence Viewer

Length: 1377 bp
ATGGCTTCATCCTCTACTTATGACGTGTTTTTGAGCTTCAGGGGCATAGACACTTACGAAGGCTTCACCAGCCACCTATATGATGCTTTGAAGCGTGGTAGTTTACTGACTTTTGCAGCTGATAGAGATCTTGAAGTTGGAACAACCATTAGCCCCAAACTGCTTAAAGCAATCCAAAGGTCTAGGATTTCTGTCATCGTTATGTCAGGGAGTTATGCATTTTCGGGATGGTGTCTTAATGAACTGTTTTGCATCATGGAATCATGGAGAAGGGAAGATAGATTGGTTTTGCCAATATTTTATAATGTCGATCCATCCGACGTCAGGAAACAGGAGGGTGGTTTTGGTGAGGCACTAGAGGAGCATCATCGTTCCGGTCGGATAGAGCAAACAACAATGCTTAAGTGGAGGGAGGCTCTCACTCAAGTTGCCAATATATGCGGCTGGGATACCAAAAATGAGAGGCACTCAGAGTTAGTTTATGACATTGTAAAGTCTATCCAAAGTAAAGGACTTCCGACAAGGCTGAACATTTCCGTCTATCCCCTCAGATATGATACGTGTGATGAATATCTCAAAGGTTTTTTGCAAGTAAGATCACAGGAAGTTGTTATAGTAGGGATCAGCGGACCAGGCCAAATGGGAAAGACAGCTATGGCCAGAGTTATGTATGACCAAATTCTACAAAATTTTGATGGTGGCTGCTTCCTCGCAGATGTTGGCCAAAGATCAAATCAACCCAACTATCTGGTTCATCTACAAGAAACACTTCTTTCTGAAATTCTCTCAGAGAGGAATGTAAAGATCTCAGATACTAGAATGGGAATAGATCTGATTGCACAAAATGTGTGCTCCAAAAGGGTTTTAGTTGTTTTGGACAATGTGGATCATTTGAGGCAAATCTATGAACTGGTTGGAGATTGCAAGTTTTTCGGTCCGGGAAGTACAATCATCATAACGACTAGAGATGTGCAACTGCTAAACCTGTTTGGAGTGGATCAGTTCATTTTAGCTCAAGGCCGTCCTCTGCCGGAAAATGTTGGGACCATCTGGGAGCCATATCAGCGAAGTCGCTGCGTAGTTGCAGGACTAAAGGAGTACATTGAGACCTTTAATCAGCAGAGAGAAGAGAGGCTGAGGTACCTTCAAGCTGTTTTGCTCCAAGAGCGCCAGAAAAACGCAATTCAGGAAAGATTAATTTTAGAGTTGCTTGAATCCCATGGTGAGGGCCGTGCCTCTACTGCTTACACAAGGACAACAGAGACAGTGATGGACAGAAATGGCTCCAATGTAGTATCGATCAACCAGAAAATGAGGATCGATCTTGAACATGAGTTAACAAAAAGATTGGGAGGTCTTCATCTGACACAGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

459

Amino Acids

52.19

Weight (kDa)

6.2

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 7 - 173 3.6e-46 TIR domain
TIR_2 PF13676 9 - 104 6.2e-09 TIR domain
NB-ARC PF00931 193 - 329 8.4e-10 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49490 FvH4_6g49490 FvH4_6g49490 FvH4_6g49490 FvH4_6g49490 FvH4_6g49490
rosa_laevigata RLG00000021861
rosa_multiflora Rmu_sc0000648.1_g000013
rosa_roxburghii Rroxscaffold_2G00082150
rosa_rugosa Rorug02G0539800
rosa_wichuraiana Rw2G050710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AatII GACGTC 1 cut(s) 324
Acc65I GGTACC 1 cut(s) 1140
AccB1I GGYRCC 1 cut(s) 1140
AciI CCGC 2 cut(s) 441, 627
AclWI GGATC 5 cut(s) 305, 629, 894, 1005, 1325
AcoI YGGCCR 2 cut(s) 657, 721
AcsI RAATTY 3 cut(s) 678, 688, 780
AcuI CTGAAG 1 cut(s) 22
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 3 cut(s) 946, 1100, 1142
AfiI CCNNNNNNNGG 2 cut(s) 324, 1225
AflII CTTAAG 1 cut(s) 401
AflIII ACRYGT 2 cut(s) 24, 560
AgsI TTSAA 5 cut(s) 91, 134, 1148, 1214, 1328
AjiI CACGTC 1 cut(s) 25
AjnI CCWGG 1 cut(s) 631
AluBI AGCT 5 cut(s) 36, 119, 653, 1013, 1151
AluI AGCT 5 cut(s) 36, 119, 653, 1013, 1151
Alw21I GWGCWC 1 cut(s) 854
Alw26I GTCTC 2 cut(s) 1100, 1256
AlwI GGATC 5 cut(s) 305, 629, 894, 1005, 1325
AoxI GGCC 5 cut(s) 634, 657, 721, 1018, 1228
ApeKI GCWGC 3 cut(s) 116, 702, 1074
ApoI RAATTY 3 cut(s) 678, 688, 780
AseI ATTAAT 1 cut(s) 1196
Asp700I GAANNNNTTC 1 cut(s) 768
Asp718I GGTACC 1 cut(s) 1140
AspLEI GCGC 1 cut(s) 1170
AspS9I GGNCC 4 cut(s) 629, 935, 1044, 1228
AsuC2I CCSGG 1 cut(s) 939
AsuHPI GGTGA 3 cut(s) 58, 359, 1235
AvaII GGWCC 3 cut(s) 629, 935, 1044
BalI TGGCCA 2 cut(s) 659, 723
BanI GGYRCC 1 cut(s) 1140
BbsI GAAGAC 1 cut(s) 1349
Bbv12I GWGCWC 1 cut(s) 854
BbvCI CCTCAGC 1 cut(s) 1136
BbvI GCAGC 3 cut(s) 128, 689, 1061
BccI CCATC 5 cut(s) 222, 322, 689, 1055, 1264
BceAI ACGGC 2 cut(s) 1005, 1215
BcgI CGANNNNNNTGC 4 cut(s) 913, 947, 1056, 1090
BciT130I CCWGG 1 cut(s) 633
BciVI GTATCC 1 cut(s) 442
BcnI CCSGG 1 cut(s) 939
BcoDI GTCTC 2 cut(s) 1100, 1256
BfaI CTAG 4 cut(s) 183, 356, 816, 963
BfoI RGCGCY 1 cut(s) 1171
BfrI CTTAAG 1 cut(s) 401
BfuI GTATCC 1 cut(s) 442
BglII AGATCT 3 cut(s) 127, 804, 829
BisI GCNGC 4 cut(s) 117, 442, 703, 1075
BlsI GCNGC 4 cut(s) 118, 443, 704, 1076
Bme1390I CCNGG 2 cut(s) 633, 939
Bme18I GGWCC 3 cut(s) 629, 935, 1044
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 4 cut(s) 629, 935, 1044, 1228
BmiI GGNNCC 4 cut(s) 1045, 1056, 1142, 1285
BmrFI CCNGG 2 cut(s) 633, 939
BmsI GCATC 3 cut(s) 73, 261, 373
BpiI GAAGAC 1 cut(s) 1349
BplI GAGNNNNNCTC 4 cut(s) 400, 432, 452, 484
Bpu10I CCTNAGC 1 cut(s) 1136
BpuEI CTTGAG 2 cut(s) 408, 999
BpuMI CCSGG 1 cut(s) 939
Bsa29I ATCGAT 2 cut(s) 1298, 1320
BsaAI YACGTR 1 cut(s) 561
BsaBI GATNNNNATC 2 cut(s) 126, 570
BsaHI GRCGYC 1 cut(s) 321
BsaI GGTCTC 1 cut(s) 1100
BsaJI CCNNGG 1 cut(s) 1219
BsaWI WCCGGW 1 cut(s) 374
Bsc4I CCNNNNNNNGG 2 cut(s) 324, 1225
Bse1I ACTGG 1 cut(s) 915
Bse8I GATNNNNATC 2 cut(s) 126, 570
BseBI CCWGG 1 cut(s) 633
BseCI ATCGAT 2 cut(s) 1298, 1320
BseDI CCNNGG 1 cut(s) 1219
BseGI GGATG 3 cut(s) 8, 233, 314
BseJI GATNNNNATC 2 cut(s) 126, 570
BseLI CCNNNNNNNGG 2 cut(s) 324, 1225
BseMII CTCAG 5 cut(s) 483, 562, 801, 822, 1127
BseNI ACTGG 1 cut(s) 915
BseRI GAGGAG 1 cut(s) 374
BseXI GCAGC 3 cut(s) 128, 689, 1061
BseYI CCCAGC 1 cut(s) 444
Bsh1285I CGRYCG 1 cut(s) 379
BshFI GGCC 5 cut(s) 636, 659, 723, 1020, 1230
BshNI GGYRCC 1 cut(s) 1140
BshVI ATCGAT 2 cut(s) 1298, 1320
BsiEI CGRYCG 1 cut(s) 379
BsiHKAI GWGCWC 1 cut(s) 854
BsiSI CCGG 3 cut(s) 375, 938, 1031
BslFI GGGAC 1 cut(s) 1057
BslI CCNNNNNNNGG 2 cut(s) 324, 1225
BsmAI GTCTC 2 cut(s) 1100, 1256
BsmFI GGGAC 1 cut(s) 1057
BsnI GGCC 5 cut(s) 636, 659, 723, 1020, 1230
Bso31I GGTCTC 1 cut(s) 1100
Bsp1286I GDGCHC 1 cut(s) 854
Bsp19I CCATGG 1 cut(s) 1219
BspACI CCGC 2 cut(s) 441, 627
BspANI GGCC 5 cut(s) 636, 659, 723, 1020, 1230
BspCNI CTCAG 5 cut(s) 482, 561, 800, 821, 1128
BspDI ATCGAT 2 cut(s) 1298, 1320
BspLI GGNNCC 4 cut(s) 1045, 1056, 1142, 1285
BspPI GGATC 5 cut(s) 305, 629, 894, 1005, 1325
BspT107I GGYRCC 1 cut(s) 1140
BspTI CTTAAG 1 cut(s) 401
BspTNI GGTCTC 1 cut(s) 1100
BsrI ACTGG 1 cut(s) 915
BssECI CCNNGG 1 cut(s) 1219
BssNI GRCGYC 1 cut(s) 321
BssT1I CCWWGG 1 cut(s) 1219
Bst2UI CCWGG 1 cut(s) 633
Bst4CI ACNGT 2 cut(s) 246, 1267
Bst6I CTCTTC 1 cut(s) 1122
BstACI GRCGYC 1 cut(s) 321
BstAFI CTTAAG 1 cut(s) 401
BstBAI YACGTR 1 cut(s) 561
BstDEI CTNAG 5 cut(s) 469, 548, 787, 808, 1136
BstDSI CCRYGG 1 cut(s) 1219
BstF5I GGATG 3 cut(s) 8, 233, 314
BstH2I RGCGCY 1 cut(s) 1171
BstHHI GCGC 1 cut(s) 1170
BstMAI GTCTC 2 cut(s) 1100, 1256
BstMCI CGRYCG 1 cut(s) 379
BstMWI GCNNNNNNNGC 6 cut(s) 42, 69, 633, 1063, 1165, 1241
BstNI CCWGG 1 cut(s) 633
BstSCI CCNGG 2 cut(s) 631, 937
BstV1I GCAGC 3 cut(s) 128, 689, 1061
BstV2I GAAGAC 1 cut(s) 1349
BstX2I RGATCY 3 cut(s) 127, 804, 829
BstXI CCANNNNNNTGG 1 cut(s) 748
BstYI RGATCY 3 cut(s) 127, 804, 829
Bsu15I ATCGAT 2 cut(s) 1298, 1320
BsuI GTATCC 1 cut(s) 442
BsuRI GGCC 5 cut(s) 636, 659, 723, 1020, 1230
BsuTUI ATCGAT 2 cut(s) 1298, 1320
BtgI CCRYGG 1 cut(s) 1219
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 3 cut(s) 8, 233, 314
BtsIMutI CAGTG 1 cut(s) 1272
CfoI GCGC 1 cut(s) 1170
Cfr13I GGNCC 4 cut(s) 629, 935, 1044, 1228
ClaI ATCGAT 2 cut(s) 1298, 1320
CpoI CGGWCCG 1 cut(s) 935
Csp6I GTAC 3 cut(s) 945, 1099, 1141
CspI CGGWCCG 1 cut(s) 935
CviAII CATG 5 cut(s) 256, 264, 1220, 1331, 1374
CviQI GTAC 3 cut(s) 945, 1099, 1141
DdeI CTNAG 5 cut(s) 469, 548, 787, 808, 1136
EaeI YGGCCR 2 cut(s) 657, 721
Eam1104I CTCTTC 1 cut(s) 1122
EarI CTCTTC 1 cut(s) 1122
Eco130I CCWWGG 1 cut(s) 1219
Eco31I GGTCTC 1 cut(s) 1100
Eco47I GGWCC 3 cut(s) 629, 935, 1044
Eco57I CTGAAG 1 cut(s) 22
EcoRII CCWGG 1 cut(s) 631
EcoT14I CCWWGG 1 cut(s) 1219
EcoT22I ATGCAT 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 1219
FaeI CATG 5 cut(s) 259, 267, 1223, 1334, 1377
FalI AAGNNNNNCTT 2 cut(s) 753, 785
FaqI GGGAC 1 cut(s) 1057
FatI CATG 5 cut(s) 255, 263, 1219, 1330, 1373
Fnu4HI GCNGC 4 cut(s) 117, 442, 703, 1075
FokI GGATG 2 cut(s) 240, 301
Fsp4HI GCNGC 4 cut(s) 117, 442, 703, 1075
FspBI CTAG 4 cut(s) 183, 356, 816, 963
GlaI GCGC 1 cut(s) 1169
GluI GCNGC 4 cut(s) 117, 442, 703, 1075
GsaI CCCAGC 1 cut(s) 448
HaeII RGCGCY 1 cut(s) 1171
HaeIII GGCC 5 cut(s) 636, 659, 723, 1020, 1230
HapII CCGG 3 cut(s) 375, 938, 1031
HhaI GCGC 1 cut(s) 1170
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 5 cut(s) 259, 267, 1223, 1334, 1377
Hin6I GCGC 1 cut(s) 1168
HinP1I GCGC 1 cut(s) 1168
HincII GTYRAC 1 cut(s) 1338
HindII GTYRAC 1 cut(s) 1338
HinfI GANTC 2 cut(s) 260, 1214
HpaI GTTAAC 1 cut(s) 1338
HpaII CCGG 3 cut(s) 375, 938, 1031
HphI GGTGA 3 cut(s) 58, 359, 1235
Hpy166II GTNNAC 2 cut(s) 104, 1338
Hpy188III TCNNGA 5 cut(s) 131, 225, 325, 1187, 1325
Hpy8I GTNNAC 2 cut(s) 104, 1338
Hpy99I CGWCG 1 cut(s) 323
HpyAV CCTTC 3 cut(s) 53, 264, 1154
HpyCH4III ACNGT 2 cut(s) 246, 1267
HpyCH4IV ACGT 3 cut(s) 24, 321, 560
HpyCH4V TGCA 8 cut(s) 116, 218, 252, 589, 839, 924, 973, 1085
HpyF10VI GCNNNNNNNGC 6 cut(s) 42, 69, 633, 1063, 1165, 1241
HpyF3I CTNAG 5 cut(s) 469, 548, 787, 808, 1136
HpySE526I ACGT 3 cut(s) 24, 321, 560
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 5 cut(s) 259, 267, 1223, 1334, 1377
HspAI GCGC 1 cut(s) 1168
KpnI GGTACC 1 cut(s) 1144
KspAI GTTAAC 1 cut(s) 1338
LmnI GCTCC 5 cut(s) 361, 857, 1054, 1164, 1289
Lsp1109I GCAGC 3 cut(s) 128, 689, 1061
LweI GCATC 3 cut(s) 73, 261, 373
MaeI CTAG 4 cut(s) 183, 356, 816, 963
MaeII ACGT 3 cut(s) 24, 321, 560
MboII GAAGA 3 cut(s) 287, 1139, 1349
MflI RGATCY 3 cut(s) 127, 804, 829
MhlI GDGCHC 1 cut(s) 854
MlsI TGGCCA 2 cut(s) 659, 723
MluCI AATT 5 cut(s) 678, 688, 780, 1182, 1197
MluNI TGGCCA 2 cut(s) 659, 723
MmeI TCCRAC 5 cut(s) 118, 342, 359, 542, 895
Mox20I TGGCCA 2 cut(s) 659, 723
Mph1103I ATGCAT 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 768
MscI TGGCCA 2 cut(s) 659, 723
MseI TTAA 6 cut(s) 165, 237, 402, 1113, 1196, 1337
MslI CAYNNNNRTG 3 cut(s) 78, 200, 1372
Msp20I TGGCCA 2 cut(s) 659, 723
MspA1I CMGCKG 2 cut(s) 119, 627
MspCI CTTAAG 1 cut(s) 401
MspI CCGG 3 cut(s) 375, 938, 1031
MspR9I CCNGG 2 cut(s) 633, 939
MvaI CCWGG 1 cut(s) 633
MwoI GCNNNNNNNGC 6 cut(s) 42, 69, 633, 1063, 1165, 1241
NciI CCSGG 1 cut(s) 939
NcoI CCATGG 1 cut(s) 1219
NlaIII CATG 5 cut(s) 259, 267, 1223, 1334, 1377
NlaIV GGNNCC 4 cut(s) 1045, 1056, 1142, 1285
NsiI ATGCAT 1 cut(s) 220
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 2 cut(s) 260, 1214
PfoI TCCNGGA 1 cut(s) 937
PkrI GCNGC 4 cut(s) 118, 443, 704, 1076
Ppu21I YACGTR 1 cut(s) 561
PshBI ATTAAT 1 cut(s) 1196
PsiI TTATAA 1 cut(s) 303
Psp6I CCWGG 1 cut(s) 631
PspFI CCCAGC 1 cut(s) 444
PspGI CCWGG 1 cut(s) 631
PspN4I GGNNCC 4 cut(s) 1045, 1056, 1142, 1285
PspPI GGNCC 4 cut(s) 629, 935, 1044, 1228
PsuI RGATCY 3 cut(s) 127, 804, 829
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 3 cut(s) 946, 1100, 1142
RsaNI GTAC 3 cut(s) 945, 1099, 1141
RseI CAYNNNNRTG 3 cut(s) 78, 200, 1372
Rsr2I CGGWCCG 1 cut(s) 935
RsrII CGGWCCG 1 cut(s) 935
SaqAI TTAA 6 cut(s) 165, 237, 402, 1113, 1196, 1337
SatI GCNGC 4 cut(s) 117, 442, 703, 1075
Sau96I GGNCC 4 cut(s) 629, 935, 1044, 1228
ScrFI CCNGG 2 cut(s) 633, 939
SduI GDGCHC 1 cut(s) 854
SfaNI GCATC 3 cut(s) 73, 261, 373
SinI GGWCC 3 cut(s) 629, 935, 1044
SmiMI CAYNNNNRTG 3 cut(s) 78, 200, 1372
SmlI CTYRAG 3 cut(s) 401, 423, 1014
SmoI CTYRAG 3 cut(s) 401, 423, 1014
Sse9I AATT 5 cut(s) 678, 688, 780, 1182, 1197
SsiI CCGC 2 cut(s) 441, 627
SspI AATATT 1 cut(s) 297
SspMI CTAG 4 cut(s) 183, 356, 816, 963
StyD4I CCNGG 2 cut(s) 631, 937
StyI CCWWGG 1 cut(s) 1219
TaaI ACNGT 2 cut(s) 246, 1267
TaiI ACGT 3 cut(s) 27, 324, 563
TaqI TCGA 3 cut(s) 309, 1298, 1320
TaqII GACCGA 1 cut(s) 923
TasI AATT 5 cut(s) 678, 688, 780, 1182, 1197
TatI WGTACW 2 cut(s) 944, 1098
TauI GCSGC 1 cut(s) 444
TfiI GAWTC 2 cut(s) 260, 1214
Tru1I TTAA 6 cut(s) 165, 237, 402, 1113, 1196, 1337
Tru9I TTAA 6 cut(s) 165, 237, 402, 1113, 1196, 1337
TscAI CASTG 1 cut(s) 1272
TseI GCWGC 3 cut(s) 116, 702, 1074
TspDTI ATGAA 6 cut(s) 255, 582, 743, 921, 994, 1349
TspGWI ACGGA 1 cut(s) 526
TspRI CASTG 1 cut(s) 1272
Vha464I CTTAAG 1 cut(s) 401
VpaK11BI GGWCC 3 cut(s) 629, 935, 1044
VspI ATTAAT 1 cut(s) 1196
XapI RAATTY 3 cut(s) 678, 688, 780
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 4 cut(s) 183, 356, 816, 963
ZraI GACGTC 1 cut(s) 322
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.