Rroxscaffold_2G00082150

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018067
Physical Location & Seq
Forward (+)
5009986 .. 5012685
2700 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00082150.1

Sequence Viewer

Length: 1431 bp
ATGGCTTCATCCTCAACTTATGACGTGCATTTGAGCTTCAGGGGTGCAGACACTCGCAAAGGCATCACAATCGACTTATATGATTTTCTGATGCGTAATAGTTTAAGGGCGTTTATGGATGATCCAGGCCGTCAAGTTGGACCAATCATTAGCCCCAAACTGCTTAAAGCAATCCAAGGGTCTAGGATTTCTGTCATCGTTATGTCAGGGAGTTATGCATCTTCGGCATGGCGTCTTAATGAACTGGTTTGCGTCATGGAATTATGGAGAAGGGAAGATAGATTGGTTTTGCCAATATTTTATAACGTCGACCCGTCCGACGTCAGGAAACAGAAGGGTAGTTTTGGTGAGGCACTAGAGGAGCATCATCGTTCCGGTCTGATAGAGCAAACAACCATGCTTAAGTGGAGGGAGGCTCTCACTCAAGTTGCCAATATATGCGGCTGGGATAAAAAAAATTATTGGACGGAAAGAGAGTTACTTGATGACCTTTTAAAGTCTATCCAAAGCAAAGGACTTCCGACAAGGCTGAACATTTCCATCTATCCCCTCAGATATGATATGTGTGATGAATATCTCAAAGTTTTTTTGCAAGCAGGATCACAGGAAGTTGTTATAGTAGGGATCTGCGGACCAGGCCAAATGGGAAAGACAGCTATGGCCAGAGTTATGTATGACCAAATTTTACACAATTTTGATGGCGGCTGCTTCCTCACAGATGTTGGCCAAAGATCAGATCAACCCAACTATCTGGTTCGTCTACAAGAAACACTTCTCTCTGAAATTCTCTCCGAAAGGAATGTAAAGATCTCAGATACTAGAATGGGAATAGATCTGATTGCACAAAATTTGTGCTCCAAAAGGGTTTTAGTTGTTCTGGACAATGTGGATGATTTTAAGCAAATATATGCACTGGTTGGAGATCGCAAGTTTTTCGGTCCTGGAAGTAAAATCATCTTAACCACTAGACATGTGCAACTGCTAGAACTGTTTGGAGTGGATCAGTTCATTTTGGCTCAAGGCCTTCCTCTGCCAGAAAATGTTGGGGCCATCTGGGAGCCATATCAGCGAAGTCGTTGTGTAGTTGCAGGACTAAAGGAGTACATTGAGACCTTTAATCAGCAGAGACTAGACAAGTTGAGGTACCTTCAAGCTGTTTTGCTCCAAGAGCGCCAGAAAAACGCAACTCAGAAAAGATTAATTTCAGAGTTGCTTGAAGCCCATGGTGAGGGCAGTTCCTCTACTGCTTACACAACGACAACAAACAGTGATGGATGGAAATGGCTCCAATGGATGTTGATCAACCAGAAAACGAGGATCGATCATGAACATGAGTTAACAAAAAGATTGGGAGGTCTTCACCTGACACAGACAACGAAACATGAAACTGGAGGTAAAGCAAAGGCCGCCTATAGGCACGGACCTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

476

Amino Acids

54.28

Weight (kDa)

8.65

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 7 - 173 5.3e-41 TIR domain
TIR_2 PF13676 9 - 105 5.5e-06 TIR domain
NB-ARC PF00931 197 - 329 3.4e-11 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49490 FvH4_6g49490 FvH4_6g49490 FvH4_6g49490 FvH4_6g49490 FvH4_6g49490
rosa_laevigata RLG00000021861
rosa_multiflora Rmu_sc0000648.1_g000013
rosa_roxburghii Rroxscaffold_2G00082150
rosa_rugosa Rorug02G0539800
rosa_wichuraiana Rw2G050710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AatII GACGTC 1 cut(s) 324
Acc65I GGTACC 1 cut(s) 1143
AccB1I GGYRCC 1 cut(s) 1143
AccI GTMKAC 2 cut(s) 309, 760
AciI CCGC 4 cut(s) 441, 630, 702, 1407
AclWI GGATC 5 cut(s) 116, 607, 632, 1008, 1325
AcoI YGGCCR 2 cut(s) 660, 724
AcsI RAATTY 3 cut(s) 681, 783, 847
AcuI CTGAAG 1 cut(s) 22
AcyI GRCGYC 2 cut(s) 232, 321
AfaI GTAC 2 cut(s) 1103, 1145
AfiI CCNNNNNNNGG 3 cut(s) 324, 1228, 1413
AflII CTTAAG 1 cut(s) 401
AflIII ACRYGT 1 cut(s) 970
AgsI TTSAA 2 cut(s) 1151, 1217
AjiI CACGTC 1 cut(s) 25
AjnI CCWGG 3 cut(s) 124, 634, 940
AluBI AGCT 3 cut(s) 36, 656, 1154
AluI AGCT 3 cut(s) 36, 656, 1154
Alw21I GWGCWC 1 cut(s) 857
Alw26I GTCTC 2 cut(s) 1103, 1120
AlwI GGATC 5 cut(s) 116, 607, 632, 1008, 1325
AoxI GGCC 7 cut(s) 127, 637, 660, 724, 1021, 1047, 1404
ApeKI GCWGC 1 cut(s) 705
ApoI RAATTY 3 cut(s) 681, 783, 847
AseI ATTAAT 1 cut(s) 1199
Asp700I GAANNNNTTC 1 cut(s) 771
Asp718I GGTACC 1 cut(s) 1143
AspLEI GCGC 1 cut(s) 1173
AspS9I GGNCC 5 cut(s) 140, 632, 938, 1047, 1421
AsuHPI GGTGA 3 cut(s) 359, 1238, 1352
AvaII GGWCC 4 cut(s) 140, 632, 938, 1421
BalI TGGCCA 2 cut(s) 662, 726
BanI GGYRCC 1 cut(s) 1143
BbsI GAAGAC 1 cut(s) 1349
Bbv12I GWGCWC 1 cut(s) 857
BbvI GCAGC 1 cut(s) 692
BccI CCATC 5 cut(s) 548, 692, 1058, 1265, 1269
BceAI ACGGC 1 cut(s) 114
BcgI CGANNNNNNTGC 4 cut(s) 52, 86, 916, 950
BciT130I CCWGG 3 cut(s) 126, 636, 942
BclI TGATCA 1 cut(s) 1299
BcoDI GTCTC 2 cut(s) 1103, 1120
BfaI CTAG 7 cut(s) 183, 356, 819, 966, 983, 1130, 1429
BfmI CTRYAG 1 cut(s) 1411
BfoI RGCGCY 1 cut(s) 1174
BfrI CTTAAG 1 cut(s) 401
BglII AGATCT 2 cut(s) 807, 832
BisI GCNGC 4 cut(s) 442, 703, 706, 1407
BlsI GCNGC 4 cut(s) 443, 704, 707, 1408
Bme1390I CCNGG 3 cut(s) 126, 636, 942
Bme18I GGWCC 4 cut(s) 140, 632, 938, 1421
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 5 cut(s) 140, 632, 938, 1047, 1421
BmiI GGNNCC 4 cut(s) 1048, 1059, 1145, 1286
BmrFI CCNGG 3 cut(s) 126, 636, 942
BmsI GCATC 4 cut(s) 72, 81, 227, 373
BpiI GAAGAC 1 cut(s) 1349
BplI GAGNNNNNCTC 2 cut(s) 400, 432
BpmI CTGGAG 1 cut(s) 1410
BpuEI CTTGAG 2 cut(s) 408, 1002
Bsa29I ATCGAT 1 cut(s) 1320
BsaBI GATNNNNATC 2 cut(s) 573, 1298
BsaHI GRCGYC 2 cut(s) 232, 321
BsaI GGTCTC 1 cut(s) 1103
BsaJI CCNNGG 2 cut(s) 175, 1222
BsaWI WCCGGW 1 cut(s) 374
Bsc4I CCNNNNNNNGG 3 cut(s) 324, 1228, 1413
Bse1I ACTGG 3 cut(s) 249, 918, 1393
Bse8I GATNNNNATC 2 cut(s) 573, 1298
BseBI CCWGG 3 cut(s) 126, 636, 942
BseCI ATCGAT 1 cut(s) 1320
BseDI CCNNGG 2 cut(s) 175, 1222
BseGI GGATG 5 cut(s) 8, 124, 895, 1280, 1299
BseJI GATNNNNATC 2 cut(s) 573, 1298
BseLI CCNNNNNNNGG 3 cut(s) 324, 1228, 1413
BseMII CTCAG 3 cut(s) 565, 825, 1202
BseNI ACTGG 3 cut(s) 249, 918, 1393
BseRI GAGGAG 1 cut(s) 374
BseXI GCAGC 1 cut(s) 692
BseYI CCCAGC 1 cut(s) 444
BsgI GTGCAG 1 cut(s) 66
BshFI GGCC 7 cut(s) 129, 639, 662, 726, 1023, 1049, 1406
BshNI GGYRCC 1 cut(s) 1143
BshVI ATCGAT 1 cut(s) 1320
BsiHKAI GWGCWC 1 cut(s) 857
BsiSI CCGG 1 cut(s) 375
BslI CCNNNNNNNGG 3 cut(s) 324, 1228, 1413
BsmAI GTCTC 2 cut(s) 1103, 1120
BsnI GGCC 7 cut(s) 129, 639, 662, 726, 1023, 1049, 1406
Bso31I GGTCTC 1 cut(s) 1103
Bsp1286I GDGCHC 1 cut(s) 857
Bsp19I CCATGG 1 cut(s) 1222
BspACI CCGC 4 cut(s) 441, 630, 702, 1407
BspANI GGCC 7 cut(s) 129, 639, 662, 726, 1023, 1049, 1406
BspCNI CTCAG 3 cut(s) 564, 824, 1201
BspDI ATCGAT 1 cut(s) 1320
BspHI TCATGA 1 cut(s) 1324
BspLI GGNNCC 4 cut(s) 1048, 1059, 1145, 1286
BspPI GGATC 5 cut(s) 116, 607, 632, 1008, 1325
BspT107I GGYRCC 1 cut(s) 1143
BspTI CTTAAG 1 cut(s) 401
BspTNI GGTCTC 1 cut(s) 1103
BsrI ACTGG 3 cut(s) 249, 918, 1393
BssECI CCNNGG 2 cut(s) 175, 1222
BssNI GRCGYC 2 cut(s) 232, 321
BssT1I CCWWGG 2 cut(s) 175, 1222
Bst2UI CCWGG 3 cut(s) 126, 636, 942
Bst4CI ACNGT 2 cut(s) 990, 1268
BstACI GRCGYC 2 cut(s) 232, 321
BstAFI CTTAAG 1 cut(s) 401
BstC8I GCNNGC 1 cut(s) 594
BstDEI CTNAG 3 cut(s) 551, 811, 1188
BstDSI CCRYGG 1 cut(s) 1222
BstF5I GGATG 5 cut(s) 8, 124, 895, 1280, 1299
BstH2I RGCGCY 1 cut(s) 1174
BstHHI GCGC 1 cut(s) 1173
BstMAI GTCTC 2 cut(s) 1103, 1120
BstMWI GCNNNNNNNGC 5 cut(s) 224, 636, 1066, 1168, 1406
BstNI CCWGG 3 cut(s) 126, 636, 942
BstNSI RCATGY 1 cut(s) 974
BstSCI CCNGG 3 cut(s) 124, 634, 940
BstSFI CTRYAG 1 cut(s) 1411
BstV1I GCAGC 1 cut(s) 692
BstV2I GAAGAC 1 cut(s) 1349
BstX2I RGATCY 3 cut(s) 624, 807, 832
BstXI CCANNNNNNTGG 1 cut(s) 751
BstYI RGATCY 3 cut(s) 624, 807, 832
Bsu15I ATCGAT 1 cut(s) 1320
BsuRI GGCC 7 cut(s) 129, 639, 662, 726, 1023, 1049, 1406
BsuTUI ATCGAT 1 cut(s) 1320
BtgI CCRYGG 1 cut(s) 1222
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 5 cut(s) 8, 124, 895, 1280, 1299
BtsIMutI CAGTG 2 cut(s) 911, 1273
Cac8I GCNNGC 1 cut(s) 594
CciI TCATGA 1 cut(s) 1324
CfoI GCGC 1 cut(s) 1173
Cfr13I GGNCC 5 cut(s) 140, 632, 938, 1047, 1421
ClaI ATCGAT 1 cut(s) 1320
CseI GACGC 2 cut(s) 221, 241
Csp6I GTAC 2 cut(s) 1102, 1144
CviAII CATG 8 cut(s) 228, 256, 397, 971, 1223, 1325, 1331, 1382
CviQI GTAC 2 cut(s) 1102, 1144
DdeI CTNAG 3 cut(s) 551, 811, 1188
DraI TTTAAA 1 cut(s) 495
EaeI YGGCCR 2 cut(s) 660, 724
Eco130I CCWWGG 2 cut(s) 175, 1222
Eco147I AGGCCT 1 cut(s) 1023
Eco31I GGTCTC 1 cut(s) 1103
Eco47I GGWCC 4 cut(s) 140, 632, 938, 1421
Eco57I CTGAAG 1 cut(s) 22
EcoRII CCWGG 3 cut(s) 124, 634, 940
EcoT14I CCWWGG 2 cut(s) 175, 1222
EcoT22I ATGCAT 1 cut(s) 220
ErhI CCWWGG 2 cut(s) 175, 1222
FaeI CATG 8 cut(s) 231, 259, 400, 974, 1226, 1328, 1334, 1385
FalI AAGNNNNNCTT 2 cut(s) 756, 788
FatI CATG 8 cut(s) 227, 255, 396, 970, 1222, 1324, 1330, 1381
FbaI TGATCA 1 cut(s) 1299
FblI GTMKAC 2 cut(s) 309, 760
Fnu4HI GCNGC 4 cut(s) 442, 703, 706, 1407
FokI GGATG 4 cut(s) 131, 902, 1287, 1306
Fsp4HI GCNGC 4 cut(s) 442, 703, 706, 1407
FspBI CTAG 7 cut(s) 183, 356, 819, 966, 983, 1130, 1429
GlaI GCGC 1 cut(s) 1172
GluI GCNGC 4 cut(s) 442, 703, 706, 1407
GsaI CCCAGC 1 cut(s) 448
GsuI CTGGAG 1 cut(s) 1410
HaeII RGCGCY 1 cut(s) 1174
HaeIII GGCC 7 cut(s) 129, 639, 662, 726, 1023, 1049, 1406
HapII CCGG 1 cut(s) 375
HgaI GACGC 2 cut(s) 221, 241
HhaI GCGC 1 cut(s) 1173
Hin1I GRCGYC 2 cut(s) 232, 321
Hin1II CATG 8 cut(s) 231, 259, 400, 974, 1226, 1328, 1334, 1385
Hin6I GCGC 1 cut(s) 1171
HinP1I GCGC 1 cut(s) 1171
HincII GTYRAC 2 cut(s) 310, 1338
HindII GTYRAC 2 cut(s) 310, 1338
HpaI GTTAAC 1 cut(s) 1338
HpaII CCGG 1 cut(s) 375
HphI GGTGA 3 cut(s) 359, 1238, 1352
Hpy166II GTNNAC 3 cut(s) 310, 761, 1338
Hpy188III TCNNGA 3 cut(s) 325, 878, 1325
Hpy8I GTNNAC 3 cut(s) 310, 761, 1338
Hpy99I CGWCG 2 cut(s) 311, 323
HpyAV CCTTC 4 cut(s) 264, 328, 1034, 1157
HpyCH4III ACNGT 2 cut(s) 990, 1268
HpyCH4IV ACGT 3 cut(s) 24, 306, 321
HpyCH4V TGCA 8 cut(s) 28, 47, 218, 592, 842, 911, 976, 1088
HpyF10VI GCNNNNNNNGC 5 cut(s) 224, 636, 1066, 1168, 1406
HpyF3I CTNAG 3 cut(s) 551, 811, 1188
HpySE526I ACGT 3 cut(s) 24, 306, 321
Hsp92I GRCGYC 2 cut(s) 232, 321
Hsp92II CATG 8 cut(s) 231, 259, 400, 974, 1226, 1328, 1334, 1385
HspAI GCGC 1 cut(s) 1171
KpnI GGTACC 1 cut(s) 1147
Ksp22I TGATCA 1 cut(s) 1299
KspAI GTTAAC 1 cut(s) 1338
LmnI GCTCC 5 cut(s) 361, 860, 1057, 1167, 1290
Lsp1109I GCAGC 1 cut(s) 692
LweI GCATC 4 cut(s) 72, 81, 227, 373
MaeI CTAG 7 cut(s) 183, 356, 819, 966, 983, 1130, 1429
MaeII ACGT 3 cut(s) 24, 306, 321
MaeIII GTNAC 1 cut(s) 477
MboII GAAGA 3 cut(s) 213, 287, 1349
MflI RGATCY 3 cut(s) 624, 807, 832
MhlI GDGCHC 1 cut(s) 857
MlsI TGGCCA 2 cut(s) 662, 726
MluCI AATT 7 cut(s) 260, 457, 681, 691, 783, 847, 1200
MluNI TGGCCA 2 cut(s) 662, 726
MmeI TCCRAC 4 cut(s) 118, 342, 545, 898
Mox20I TGGCCA 2 cut(s) 662, 726
Mph1103I ATGCAT 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 771
MscI TGGCCA 2 cut(s) 662, 726
MslI CAYNNNNRTG 2 cut(s) 200, 1329
Msp20I TGGCCA 2 cut(s) 662, 726
MspCI CTTAAG 1 cut(s) 401
MspI CCGG 1 cut(s) 375
MspR9I CCNGG 3 cut(s) 126, 636, 942
MvaI CCWGG 3 cut(s) 126, 636, 942
MwoI GCNNNNNNNGC 5 cut(s) 224, 636, 1066, 1168, 1406
NcoI CCATGG 1 cut(s) 1222
NlaIII CATG 8 cut(s) 231, 259, 400, 974, 1226, 1328, 1334, 1385
NlaIV GGNNCC 4 cut(s) 1048, 1059, 1145, 1286
NsiI ATGCAT 1 cut(s) 220
NspI RCATGY 1 cut(s) 974
PagI TCATGA 1 cut(s) 1324
PceI AGGCCT 1 cut(s) 1023
PciI ACATGT 1 cut(s) 970
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PdmI GAANNNNTTC 1 cut(s) 771
PfoI TCCNGGA 1 cut(s) 940
PkrI GCNGC 4 cut(s) 443, 704, 707, 1408
PscI ACATGT 1 cut(s) 970
PshBI ATTAAT 1 cut(s) 1199
PsiI TTATAA 1 cut(s) 303
Psp6I CCWGG 3 cut(s) 124, 634, 940
PspFI CCCAGC 1 cut(s) 444
PspGI CCWGG 3 cut(s) 124, 634, 940
PspN4I GGNNCC 4 cut(s) 1048, 1059, 1145, 1286
PspPI GGNCC 5 cut(s) 140, 632, 938, 1047, 1421
PsuI RGATCY 3 cut(s) 624, 807, 832
RsaI GTAC 2 cut(s) 1103, 1145
RsaNI GTAC 2 cut(s) 1102, 1144
RseI CAYNNNNRTG 2 cut(s) 200, 1329
SalI GTCGAC 1 cut(s) 308
SatI GCNGC 4 cut(s) 442, 703, 706, 1407
Sau96I GGNCC 5 cut(s) 140, 632, 938, 1047, 1421
ScrFI CCNGG 3 cut(s) 126, 636, 942
SduI GDGCHC 1 cut(s) 857
SfaNI GCATC 4 cut(s) 72, 81, 227, 373
SfcI CTRYAG 1 cut(s) 1411
SinI GGWCC 4 cut(s) 140, 632, 938, 1421
SmiMI CAYNNNNRTG 2 cut(s) 200, 1329
SmlI CTYRAG 3 cut(s) 401, 423, 1017
SmoI CTYRAG 3 cut(s) 401, 423, 1017
Sse9I AATT 7 cut(s) 260, 457, 681, 691, 783, 847, 1200
SseBI AGGCCT 1 cut(s) 1023
SsiI CCGC 4 cut(s) 441, 630, 702, 1407
SspI AATATT 1 cut(s) 297
SspMI CTAG 7 cut(s) 183, 356, 819, 966, 983, 1130, 1429
StuI AGGCCT 1 cut(s) 1023
StyD4I CCNGG 3 cut(s) 124, 634, 940
StyI CCWWGG 2 cut(s) 175, 1222
TaaI ACNGT 2 cut(s) 990, 1268
TaiI ACGT 3 cut(s) 27, 309, 324
TaqI TCGA 3 cut(s) 72, 309, 1320
TaqII GACCGA 1 cut(s) 926
TasI AATT 7 cut(s) 260, 457, 681, 691, 783, 847, 1200
TatI WGTACW 1 cut(s) 1101
TauI GCSGC 3 cut(s) 444, 705, 1409
TscAI CASTG 2 cut(s) 918, 1273
TseI GCWGC 1 cut(s) 705
TspDTI ATGAA 5 cut(s) 255, 585, 997, 1341, 1398
TspGWI ACGGA 1 cut(s) 482
TspRI CASTG 2 cut(s) 918, 1273
Vha464I CTTAAG 1 cut(s) 401
VpaK11BI GGWCC 4 cut(s) 140, 632, 938, 1421
VspI ATTAAT 1 cut(s) 1199
XapI RAATTY 3 cut(s) 681, 783, 847
XceI RCATGY 1 cut(s) 974
XmiI GTMKAC 2 cut(s) 309, 760
XmnI GAANNNNTTC 1 cut(s) 771
XspI CTAG 7 cut(s) 183, 356, 819, 966, 983, 1130, 1429
ZraI GACGTC 1 cut(s) 322
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.