Rw2G050710

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0018067
Physical Location & Seq
Reverse (-)
78339867 .. 78341625
1759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G050710.1

Sequence Viewer

Length: 1374 bp
ATGGCTTCGTCCTCTACTTATGACGTGTTTTTGAGCTTCAGGGGCATAGACACTTACGAAGGTTTCACCAGCCACCTATATGATGCTTTGAAGCGTGGTAGTTTAATGACGTTTGCAGCTGATAGAGATCTTGAAGTTGGAACAACCATTAGCCCCAAACTGCTTAAAGCAATCCAAAGGTCTAGGATTTCTGTCATCGTTATGTCAGGGAGCTATGCATTTTCGGGATGGTGTCTTAATGAACTGCTTTGCATCATGGAATCATGGAGAAGGGAAGATAGATTGGTTTTGCCAATATTTTATAATGTCGATCCATCTGACGTCAGGAAACAGAAGGGTGGTTTTGGTGAGGCACTAGAGGAGCATCATCGTTCAGGTCATATAGAGCAAACAACCATGCTTAAGTGGAGGGAGGCTCTCACTCAAGTTTCCAACATATGCGGCTGGGATACCAAAAATGAGAGGCACTCAGAGTTAGTTGATGACATTGTAAAATCTATCCAAAGTAAAGGACTTCCGACAAGGCTGAACATTTCCATCTATCCCCTCAGATATGATACGTGTGATGAATATCTCAAAGGTTTTTTGCAAGTAGGATCACAGGAAGTTGTTATAGTAGGGATCTGCGGACCAGGCCAAATGGGAAAGAGAGCTATGGCCAGAGTTATGTATGACCAAATTCTACAAAATTTTGATGGTGGCTGCTTCCTCGCAGATGTTGGCCAAAGATCAAATCAACCCAACTATCTGGTTCATCTACAAGAAACACTTCTTTCTGAAATTCTCTCAGAGAGGAATGTAAAGATCTCAGATACTAGAATGGGAATAGATCTGATTGCACAAAATTTGTGCTCCAAAAGGGTTTTAGTTGTTTTGGACAATGTGGATCATTTGAGGCAAATCTATGCACTGGTTGGAGATTGCAAGTTTTTCGGTCTGGGAAGTAAAATCATCATAACGACTAGAGATGTGCAACTGCTAAACCTGTTTGGAGTGGACCAGTTCATTTTGGCTCATGGCCGTCCTCTGCCAGAAAATGTTGGGGCCATCTGGGAGCCATATCAGCGAAGTCGTTGCGTAGTGGCAGGAATCAAGGAGTACATTGAGACCTTTAATCATCAGAGACAAGAGAGGTTGAAGTACCTTAAAGCTGTTTTTCTCCAAGAGCGCCAGAAAAACGCAACTCAGGAAAGATTAATTTTAGAGTTGCTTGAATCCCATGGTGAGGGCAGTGCCTCTACTGCTTACACAAGGACAACTGAGACAGTGATGGACAGCAATGGCTCCAATGTAGTATTGATCAACCAGAAAATGAGGATCGATCTTGAACATGATGGAGGTAAAGCAAACATCTATAGGCATGGACCTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

457

Amino Acids

51.86

Weight (kDa)

6.65

Isoelectric Point (pI)

41.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 7 - 173 5.9e-47 TIR domain
TIR_2 PF13676 9 - 105 2.3e-09 TIR domain
NB-ARC PF00931 193 - 329 1.9e-09 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49490 FvH4_6g49490 FvH4_6g49490 FvH4_6g49490 FvH4_6g49490 FvH4_6g49490
rosa_laevigata RLG00000021861
rosa_multiflora Rmu_sc0000648.1_g000013
rosa_roxburghii Rroxscaffold_2G00082150
rosa_rugosa Rorug02G0539800
rosa_wichuraiana Rw2G050710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AatII GACGTC 1 cut(s) 324
AciI CCGC 2 cut(s) 441, 627
AclWI GGATC 5 cut(s) 305, 604, 629, 894, 1325
AcoI YGGCCR 3 cut(s) 657, 721, 1018
AcsI RAATTY 4 cut(s) 678, 688, 780, 844
AcuI CTGAAG 1 cut(s) 22
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 2 cut(s) 1100, 1142
AfiI CCNNNNNNNGG 1 cut(s) 1225
AflII CTTAAG 1 cut(s) 401
AflIII ACRYGT 2 cut(s) 24, 560
AgsI TTSAA 5 cut(s) 91, 134, 1138, 1214, 1328
AjiI CACGTC 1 cut(s) 25
AjnI CCWGG 1 cut(s) 631
AluBI AGCT 5 cut(s) 36, 119, 213, 653, 1151
AluI AGCT 5 cut(s) 36, 119, 213, 653, 1151
Alw21I GWGCWC 1 cut(s) 854
Alw26I GTCTC 3 cut(s) 1100, 1117, 1256
AlwI GGATC 5 cut(s) 305, 604, 629, 894, 1325
AoxI GGCC 5 cut(s) 634, 657, 721, 1018, 1044
ApeKI GCWGC 2 cut(s) 116, 702
ApoI RAATTY 4 cut(s) 678, 688, 780, 844
AseI ATTAAT 1 cut(s) 1196
Asp700I GAANNNNTTC 1 cut(s) 768
AspLEI GCGC 1 cut(s) 1170
AspS9I GGNCC 4 cut(s) 629, 997, 1044, 1364
AsuHPI GGTGA 3 cut(s) 58, 359, 1235
AvaII GGWCC 3 cut(s) 629, 997, 1364
BalI TGGCCA 2 cut(s) 659, 723
Bbv12I GWGCWC 1 cut(s) 854
BbvI GCAGC 2 cut(s) 128, 689
BccI CCATC 7 cut(s) 222, 322, 545, 689, 1055, 1264, 1328
BceAI ACGGC 1 cut(s) 1005
BcgI CGANNNNNNTGC 4 cut(s) 913, 947, 1056, 1090
BciT130I CCWGG 1 cut(s) 633
BciVI GTATCC 1 cut(s) 442
BclI TGATCA 1 cut(s) 1299
BcoDI GTCTC 3 cut(s) 1100, 1117, 1256
BfaI CTAG 5 cut(s) 183, 356, 816, 963, 1372
BfmI CTRYAG 1 cut(s) 1354
BfoI RGCGCY 1 cut(s) 1171
BfrI CTTAAG 1 cut(s) 401
BfuI GTATCC 1 cut(s) 442
BglII AGATCT 3 cut(s) 127, 804, 829
BisI GCNGC 3 cut(s) 117, 442, 703
BlsI GCNGC 3 cut(s) 118, 443, 704
Bme1390I CCNGG 1 cut(s) 633
Bme18I GGWCC 3 cut(s) 629, 997, 1364
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 4 cut(s) 629, 997, 1044, 1364
BmiI GGNNCC 3 cut(s) 1045, 1056, 1285
BmrFI CCNGG 1 cut(s) 633
BmsI GCATC 3 cut(s) 73, 261, 373
BplI GAGNNNNNCTC 4 cut(s) 400, 432, 452, 484
BpuEI CTTGAG 1 cut(s) 408
Bsa29I ATCGAT 1 cut(s) 1320
BsaAI YACGTR 1 cut(s) 561
BsaBI GATNNNNATC 2 cut(s) 126, 570
BsaHI GRCGYC 1 cut(s) 321
BsaI GGTCTC 1 cut(s) 1100
BsaJI CCNNGG 1 cut(s) 1219
Bsc4I CCNNNNNNNGG 1 cut(s) 1225
Bse1I ACTGG 2 cut(s) 915, 1000
Bse3DI GCAATG 1 cut(s) 1285
Bse8I GATNNNNATC 2 cut(s) 126, 570
BseBI CCWGG 1 cut(s) 633
BseCI ATCGAT 1 cut(s) 1320
BseDI CCNNGG 1 cut(s) 1219
BseGI GGATG 1 cut(s) 233
BseJI GATNNNNATC 2 cut(s) 126, 570
BseLI CCNNNNNNNGG 1 cut(s) 1225
BseMI GCAATG 1 cut(s) 1285
BseMII CTCAG 6 cut(s) 483, 562, 801, 822, 1199, 1251
BseNI ACTGG 2 cut(s) 915, 1000
BseRI GAGGAG 1 cut(s) 374
BseXI GCAGC 2 cut(s) 128, 689
BseYI CCCAGC 1 cut(s) 444
BshFI GGCC 5 cut(s) 636, 659, 723, 1020, 1046
BshVI ATCGAT 1 cut(s) 1320
BsiHKAI GWGCWC 1 cut(s) 854
BslI CCNNNNNNNGG 1 cut(s) 1225
BsmAI GTCTC 3 cut(s) 1100, 1117, 1256
BsnI GGCC 5 cut(s) 636, 659, 723, 1020, 1046
Bso31I GGTCTC 1 cut(s) 1100
Bsp1286I GDGCHC 1 cut(s) 854
Bsp19I CCATGG 1 cut(s) 1219
BspACI CCGC 2 cut(s) 441, 627
BspANI GGCC 5 cut(s) 636, 659, 723, 1020, 1046
BspCNI CTCAG 6 cut(s) 482, 561, 800, 821, 1198, 1252
BspDI ATCGAT 1 cut(s) 1320
BspLI GGNNCC 3 cut(s) 1045, 1056, 1285
BspPI GGATC 5 cut(s) 305, 604, 629, 894, 1325
BspTI CTTAAG 1 cut(s) 401
BspTNI GGTCTC 1 cut(s) 1100
BsrDI GCAATG 1 cut(s) 1285
BsrI ACTGG 2 cut(s) 915, 1000
BssECI CCNNGG 1 cut(s) 1219
BssNI GRCGYC 1 cut(s) 321
BssT1I CCWWGG 1 cut(s) 1219
Bst2UI CCWGG 1 cut(s) 633
Bst4CI ACNGT 1 cut(s) 1267
BstACI GRCGYC 1 cut(s) 321
BstAFI CTTAAG 1 cut(s) 401
BstBAI YACGTR 1 cut(s) 561
BstDEI CTNAG 6 cut(s) 469, 548, 787, 808, 1185, 1260
BstDSI CCRYGG 1 cut(s) 1219
BstF5I GGATG 1 cut(s) 233
BstH2I RGCGCY 1 cut(s) 1171
BstHHI GCGC 1 cut(s) 1170
BstMAI GTCTC 3 cut(s) 1100, 1117, 1256
BstMWI GCNNNNNNNGC 4 cut(s) 42, 633, 1063, 1241
BstNI CCWGG 1 cut(s) 633
BstSCI CCNGG 1 cut(s) 631
BstSFI CTRYAG 1 cut(s) 1354
BstV1I GCAGC 2 cut(s) 128, 689
BstX2I RGATCY 4 cut(s) 127, 621, 804, 829
BstXI CCANNNNNNTGG 1 cut(s) 748
BstYI RGATCY 4 cut(s) 127, 621, 804, 829
Bsu15I ATCGAT 1 cut(s) 1320
BsuI GTATCC 1 cut(s) 442
BsuRI GGCC 5 cut(s) 636, 659, 723, 1020, 1046
BsuTUI ATCGAT 1 cut(s) 1320
BtgI CCRYGG 1 cut(s) 1219
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 1 cut(s) 233
BtsI GCAGTG 1 cut(s) 1237
BtsIMutI CAGTG 3 cut(s) 908, 1237, 1272
CfoI GCGC 1 cut(s) 1170
Cfr13I GGNCC 4 cut(s) 629, 997, 1044, 1364
ClaI ATCGAT 1 cut(s) 1320
Csp6I GTAC 2 cut(s) 1099, 1141
CviAII CATG 7 cut(s) 256, 264, 397, 1016, 1220, 1331, 1361
CviQI GTAC 2 cut(s) 1099, 1141
DdeI CTNAG 6 cut(s) 469, 548, 787, 808, 1185, 1260
EaeI YGGCCR 3 cut(s) 657, 721, 1018
Eco130I CCWWGG 1 cut(s) 1219
Eco31I GGTCTC 1 cut(s) 1100
Eco47I GGWCC 3 cut(s) 629, 997, 1364
Eco57I CTGAAG 1 cut(s) 22
EcoRII CCWGG 1 cut(s) 631
EcoT14I CCWWGG 1 cut(s) 1219
EcoT22I ATGCAT 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 1219
FaeI CATG 7 cut(s) 259, 267, 400, 1019, 1223, 1334, 1364
FalI AAGNNNNNCTT 2 cut(s) 753, 785
FatI CATG 7 cut(s) 255, 263, 396, 1015, 1219, 1330, 1360
FauNDI CATATG 1 cut(s) 437
FbaI TGATCA 1 cut(s) 1299
Fnu4HI GCNGC 3 cut(s) 117, 442, 703
FokI GGATG 1 cut(s) 240
Fsp4HI GCNGC 3 cut(s) 117, 442, 703
FspBI CTAG 5 cut(s) 183, 356, 816, 963, 1372
GlaI GCGC 1 cut(s) 1169
GluI GCNGC 3 cut(s) 117, 442, 703
GsaI CCCAGC 1 cut(s) 448
HaeII RGCGCY 1 cut(s) 1171
HaeIII GGCC 5 cut(s) 636, 659, 723, 1020, 1046
HhaI GCGC 1 cut(s) 1170
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 7 cut(s) 259, 267, 400, 1019, 1223, 1334, 1364
Hin6I GCGC 1 cut(s) 1168
HinP1I GCGC 1 cut(s) 1168
HinfI GANTC 3 cut(s) 260, 1089, 1214
HphI GGTGA 3 cut(s) 58, 359, 1235
Hpy166II GTNNAC 1 cut(s) 997
Hpy188I TCNGA 9 cut(s) 319, 472, 519, 551, 778, 790, 811, 834, 1122
Hpy188III TCNNGA 5 cut(s) 131, 225, 325, 1187, 1325
Hpy8I GTNNAC 1 cut(s) 997
HpyAV CCTTC 3 cut(s) 53, 264, 328
HpyCH4III ACNGT 1 cut(s) 1267
HpyCH4IV ACGT 4 cut(s) 24, 110, 321, 560
HpyCH4V TGCA 8 cut(s) 116, 218, 252, 589, 839, 908, 924, 973
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 633, 1063, 1241
HpyF3I CTNAG 6 cut(s) 469, 548, 787, 808, 1185, 1260
HpySE526I ACGT 4 cut(s) 24, 110, 321, 560
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 7 cut(s) 259, 267, 400, 1019, 1223, 1334, 1364
HspAI GCGC 1 cut(s) 1168
Ksp22I TGATCA 1 cut(s) 1299
LmnI GCTCC 5 cut(s) 210, 361, 857, 1054, 1289
Lsp1109I GCAGC 2 cut(s) 128, 689
LweI GCATC 3 cut(s) 73, 261, 373
MaeI CTAG 5 cut(s) 183, 356, 816, 963, 1372
MaeII ACGT 4 cut(s) 24, 110, 321, 560
MboII GAAGA 1 cut(s) 287
MflI RGATCY 4 cut(s) 127, 621, 804, 829
MhlI GDGCHC 1 cut(s) 854
MlsI TGGCCA 2 cut(s) 659, 723
MluCI AATT 5 cut(s) 678, 688, 780, 844, 1197
MluNI TGGCCA 2 cut(s) 659, 723
MmeI TCCRAC 4 cut(s) 118, 456, 542, 895
Mox20I TGGCCA 2 cut(s) 659, 723
Mph1103I ATGCAT 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 768
MscI TGGCCA 2 cut(s) 659, 723
MseI TTAA 7 cut(s) 104, 165, 237, 402, 1113, 1146, 1196
MslI CAYNNNNRTG 2 cut(s) 78, 200
Msp20I TGGCCA 2 cut(s) 659, 723
MspA1I CMGCKG 1 cut(s) 119
MspCI CTTAAG 1 cut(s) 401
MspR9I CCNGG 1 cut(s) 633
MvaI CCWGG 1 cut(s) 633
MwoI GCNNNNNNNGC 4 cut(s) 42, 633, 1063, 1241
NcoI CCATGG 1 cut(s) 1219
NdeI CATATG 1 cut(s) 437
NlaIII CATG 7 cut(s) 259, 267, 400, 1019, 1223, 1334, 1364
NlaIV GGNNCC 3 cut(s) 1045, 1056, 1285
NsiI ATGCAT 1 cut(s) 220
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 3 cut(s) 260, 1089, 1214
PkrI GCNGC 3 cut(s) 118, 443, 704
Ppu21I YACGTR 1 cut(s) 561
PshBI ATTAAT 1 cut(s) 1196
PsiI TTATAA 1 cut(s) 303
Psp6I CCWGG 1 cut(s) 631
PspFI CCCAGC 1 cut(s) 444
PspGI CCWGG 1 cut(s) 631
PspN4I GGNNCC 3 cut(s) 1045, 1056, 1285
PspPI GGNCC 4 cut(s) 629, 997, 1044, 1364
PsuI RGATCY 4 cut(s) 127, 621, 804, 829
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 2 cut(s) 1100, 1142
RsaNI GTAC 2 cut(s) 1099, 1141
RseI CAYNNNNRTG 2 cut(s) 78, 200
SaqAI TTAA 7 cut(s) 104, 165, 237, 402, 1113, 1146, 1196
SatI GCNGC 3 cut(s) 117, 442, 703
Sau96I GGNCC 4 cut(s) 629, 997, 1044, 1364
ScrFI CCNGG 1 cut(s) 633
SduI GDGCHC 1 cut(s) 854
SfaNI GCATC 3 cut(s) 73, 261, 373
SfcI CTRYAG 1 cut(s) 1354
SinI GGWCC 3 cut(s) 629, 997, 1364
SmiMI CAYNNNNRTG 2 cut(s) 78, 200
SmlI CTYRAG 2 cut(s) 401, 423
SmoI CTYRAG 2 cut(s) 401, 423
Sse9I AATT 5 cut(s) 678, 688, 780, 844, 1197
SsiI CCGC 2 cut(s) 441, 627
SspI AATATT 1 cut(s) 297
SspMI CTAG 5 cut(s) 183, 356, 816, 963, 1372
StyD4I CCNGG 1 cut(s) 631
StyI CCWWGG 1 cut(s) 1219
TaaI ACNGT 1 cut(s) 1267
TaiI ACGT 4 cut(s) 27, 113, 324, 563
TaqI TCGA 2 cut(s) 309, 1320
TaqII GACCGA 1 cut(s) 923
TasI AATT 5 cut(s) 678, 688, 780, 844, 1197
TatI WGTACW 1 cut(s) 1098
TauI GCSGC 1 cut(s) 444
TfiI GAWTC 3 cut(s) 260, 1089, 1214
Tru1I TTAA 7 cut(s) 104, 165, 237, 402, 1113, 1146, 1196
Tru9I TTAA 7 cut(s) 104, 165, 237, 402, 1113, 1146, 1196
TscAI CASTG 3 cut(s) 915, 1237, 1272
TseI GCWGC 2 cut(s) 116, 702
TspDTI ATGAA 4 cut(s) 255, 582, 743, 994
TspRI CASTG 3 cut(s) 915, 1237, 1272
Vha464I CTTAAG 1 cut(s) 401
VpaK11BI GGWCC 3 cut(s) 629, 997, 1364
VspI ATTAAT 1 cut(s) 1196
XapI RAATTY 4 cut(s) 678, 688, 780, 844
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 5 cut(s) 183, 356, 816, 963, 1372
ZraI GACGTC 1 cut(s) 322
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.