Rmu_sc0000648.1_g000013

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000648.1
OG0018067
Physical Location & Seq
Reverse (-)
65337 .. 67045
1709 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000648.1_g000013.1.cds

Sequence Viewer

Length: 1311 bp
atggcttcgtcctctacttatgacgtgtttttgagcttcaggggcatagacacttacgaaggtttcaccagccacctatatgatgctttgaagcgtggtagtttaatgacgtttgcagctgatagagatcttgaagttggaacaaccattagccccaaactgcttaaagcaatccaaaggtctaggatttctgtcatcgttatgtcagggagctatgcattttcgggatggtgtcttaatgaactgctttgcatcatggaatcatggagaagggaagatagattggttttgccaatattttataatgtcgatccatctgacgtcaggaaacagaagggtggttttggtgaggcactagaggagcatcatcgttcaggtcatatagagcaaacaaccatgcttaagtggagggaggctctcactcaagtttccaacatatgcggctgggataccaaaaatgagaggcactcagagttagttgatgacattgtaaagtctatccaaagtaaaggacttccgacaaggctgaacatttccatctatcccctcagatatgatacgtgtgatgaatatctcaaaggttttttgcaagtaggatcacaggaagttgttatagtagggatctgcggaccaggccaaatgggaaagagagctatggccagagttatgtatgaccaaattctacaaaattttgatggtggctgcttcctcgcagatgttggccaaagatcaaatcaacccaactatctggttcatctacaagaaacacttctttctgaaattctctcagagaggaatgtaaagatctcagatactagaatgggaatagatctgattgcacaaaatttgtgctccaaaagggttttagttgttttggacaatgtggatcatttgaggcaaatctatgcactggttggagattgcaagtttttcggtctgggaagtaaaatcatcataacgactagagatgtgcaactgctaaacctgtttggagtggaccagttcattttggctcatggccgtcctctgccagaaaatgttggggccatctgggagccatatcagcgaagtcgttacgtagttgcaggaatcaaggagtacattgagacctttaatcaacaggaaagattaattttagagttgcttgaatcccatggtgagggcagtgcctctactgcttacacaaggacaactgagacagtgatggacagcaatggctccaatgtagtattgatcaaccagaaaatgaggatcgatcttgaacatgagttaacaaaaagattgggaggtcttcacctgacacagacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

436

Amino Acids

49.3

Weight (kDa)

6.04

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49490 FvH4_6g49490 FvH4_6g49490 FvH4_6g49490 FvH4_6g49490 FvH4_6g49490
rosa_laevigata RLG00000021861
rosa_multiflora Rmu_sc0000648.1_g000013
rosa_roxburghii Rroxscaffold_2G00082150
rosa_rugosa Rorug02G0539800
rosa_wichuraiana Rw2G050710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AatII GACGTC 1 cut(s) 324
AciI CCGC 2 cut(s) 441, 627
AclWI GGATC 5 cut(s) 305, 604, 629, 894, 1259
AcoI YGGCCR 3 cut(s) 657, 721, 1018
AcsI RAATTY 4 cut(s) 678, 688, 780, 844
AcuI CTGAAG 1 cut(s) 22
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 1 cut(s) 1100
AfiI CCNNNNNNNGG 1 cut(s) 1159
AflII CTTAAG 1 cut(s) 401
AflIII ACRYGT 2 cut(s) 24, 560
AgsI TTSAA 4 cut(s) 91, 134, 1148, 1262
AjiI CACGTC 1 cut(s) 25
AjnI CCWGG 1 cut(s) 631
AluBI AGCT 4 cut(s) 36, 119, 213, 653
AluI AGCT 4 cut(s) 36, 119, 213, 653
Alw21I GWGCWC 1 cut(s) 854
Alw26I GTCTC 2 cut(s) 1100, 1190
AlwI GGATC 5 cut(s) 305, 604, 629, 894, 1259
AoxI GGCC 5 cut(s) 634, 657, 721, 1018, 1044
ApeKI GCWGC 2 cut(s) 116, 702
ApoI RAATTY 4 cut(s) 678, 688, 780, 844
AseI ATTAAT 1 cut(s) 1130
Asp700I GAANNNNTTC 1 cut(s) 768
AspS9I GGNCC 3 cut(s) 629, 997, 1044
AsuHPI GGTGA 4 cut(s) 58, 359, 1169, 1286
AvaII GGWCC 2 cut(s) 629, 997
BalI TGGCCA 2 cut(s) 659, 723
BbsI GAAGAC 1 cut(s) 1283
Bbv12I GWGCWC 1 cut(s) 854
BbvI GCAGC 2 cut(s) 128, 689
BccI CCATC 6 cut(s) 222, 322, 545, 689, 1055, 1198
BceAI ACGGC 1 cut(s) 1005
BcgI CGANNNNNNTGC 2 cut(s) 913, 947
BciT130I CCWGG 1 cut(s) 633
BciVI GTATCC 1 cut(s) 442
BclI TGATCA 1 cut(s) 1233
BcoDI GTCTC 2 cut(s) 1100, 1190
BfaI CTAG 4 cut(s) 183, 356, 816, 963
BfrI CTTAAG 1 cut(s) 401
BfuI GTATCC 1 cut(s) 442
BglII AGATCT 3 cut(s) 127, 804, 829
BisI GCNGC 3 cut(s) 117, 442, 703
BlsI GCNGC 3 cut(s) 118, 443, 704
Bme1390I CCNGG 1 cut(s) 633
Bme18I GGWCC 2 cut(s) 629, 997
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 3 cut(s) 629, 997, 1044
BmiI GGNNCC 3 cut(s) 1045, 1056, 1219
BmrFI CCNGG 1 cut(s) 633
BmsI GCATC 3 cut(s) 73, 261, 373
BpiI GAAGAC 1 cut(s) 1283
BplI GAGNNNNNCTC 4 cut(s) 400, 432, 452, 484
BpuEI CTTGAG 1 cut(s) 408
Bsa29I ATCGAT 1 cut(s) 1254
BsaAI YACGTR 2 cut(s) 561, 1078
BsaBI GATNNNNATC 2 cut(s) 126, 570
BsaHI GRCGYC 1 cut(s) 321
BsaI GGTCTC 1 cut(s) 1100
BsaJI CCNNGG 1 cut(s) 1153
Bsc4I CCNNNNNNNGG 1 cut(s) 1159
Bse1I ACTGG 2 cut(s) 915, 1000
Bse3DI GCAATG 1 cut(s) 1219
Bse8I GATNNNNATC 2 cut(s) 126, 570
BseBI CCWGG 1 cut(s) 633
BseCI ATCGAT 1 cut(s) 1254
BseDI CCNNGG 1 cut(s) 1153
BseGI GGATG 1 cut(s) 233
BseJI GATNNNNATC 2 cut(s) 126, 570
BseLI CCNNNNNNNGG 1 cut(s) 1159
BseMI GCAATG 1 cut(s) 1219
BseMII CTCAG 5 cut(s) 483, 562, 801, 822, 1185
BseNI ACTGG 2 cut(s) 915, 1000
BseRI GAGGAG 1 cut(s) 374
BseXI GCAGC 2 cut(s) 128, 689
BseYI CCCAGC 1 cut(s) 444
BshFI GGCC 5 cut(s) 636, 659, 723, 1020, 1046
BshVI ATCGAT 1 cut(s) 1254
BsiHKAI GWGCWC 1 cut(s) 854
BslI CCNNNNNNNGG 1 cut(s) 1159
BsmAI GTCTC 2 cut(s) 1100, 1190
BsnI GGCC 5 cut(s) 636, 659, 723, 1020, 1046
Bso31I GGTCTC 1 cut(s) 1100
Bsp1286I GDGCHC 1 cut(s) 854
Bsp19I CCATGG 1 cut(s) 1153
BspACI CCGC 2 cut(s) 441, 627
BspANI GGCC 5 cut(s) 636, 659, 723, 1020, 1046
BspCNI CTCAG 5 cut(s) 482, 561, 800, 821, 1186
BspDI ATCGAT 1 cut(s) 1254
BspLI GGNNCC 3 cut(s) 1045, 1056, 1219
BspPI GGATC 5 cut(s) 305, 604, 629, 894, 1259
BspTI CTTAAG 1 cut(s) 401
BspTNI GGTCTC 1 cut(s) 1100
BsrDI GCAATG 1 cut(s) 1219
BsrI ACTGG 2 cut(s) 915, 1000
BssECI CCNNGG 1 cut(s) 1153
BssNI GRCGYC 1 cut(s) 321
BssT1I CCWWGG 1 cut(s) 1153
Bst2UI CCWGG 1 cut(s) 633
Bst4CI ACNGT 1 cut(s) 1201
BstACI GRCGYC 1 cut(s) 321
BstAFI CTTAAG 1 cut(s) 401
BstBAI YACGTR 2 cut(s) 561, 1078
BstDEI CTNAG 5 cut(s) 469, 548, 787, 808, 1194
BstDSI CCRYGG 1 cut(s) 1153
BstF5I GGATG 1 cut(s) 233
BstMAI GTCTC 2 cut(s) 1100, 1190
BstMWI GCNNNNNNNGC 4 cut(s) 42, 633, 1063, 1175
BstNI CCWGG 1 cut(s) 633
BstSCI CCNGG 1 cut(s) 631
BstSNI TACGTA 1 cut(s) 1078
BstV1I GCAGC 2 cut(s) 128, 689
BstV2I GAAGAC 1 cut(s) 1283
BstX2I RGATCY 4 cut(s) 127, 621, 804, 829
BstXI CCANNNNNNTGG 1 cut(s) 748
BstYI RGATCY 4 cut(s) 127, 621, 804, 829
Bsu15I ATCGAT 1 cut(s) 1254
BsuI GTATCC 1 cut(s) 442
BsuRI GGCC 5 cut(s) 636, 659, 723, 1020, 1046
BsuTUI ATCGAT 1 cut(s) 1254
BtgI CCRYGG 1 cut(s) 1153
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 1 cut(s) 233
BtsI GCAGTG 1 cut(s) 1171
BtsIMutI CAGTG 3 cut(s) 908, 1171, 1206
Cfr13I GGNCC 3 cut(s) 629, 997, 1044
ClaI ATCGAT 1 cut(s) 1254
Csp6I GTAC 1 cut(s) 1099
CviAII CATG 7 cut(s) 256, 264, 397, 1016, 1154, 1265, 1308
CviQI GTAC 1 cut(s) 1099
DdeI CTNAG 5 cut(s) 469, 548, 787, 808, 1194
EaeI YGGCCR 3 cut(s) 657, 721, 1018
Eco105I TACGTA 1 cut(s) 1078
Eco130I CCWWGG 1 cut(s) 1153
Eco31I GGTCTC 1 cut(s) 1100
Eco47I GGWCC 2 cut(s) 629, 997
Eco57I CTGAAG 1 cut(s) 22
EcoRII CCWGG 1 cut(s) 631
EcoT14I CCWWGG 1 cut(s) 1153
EcoT22I ATGCAT 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 1153
FaeI CATG 7 cut(s) 259, 267, 400, 1019, 1157, 1268, 1311
FalI AAGNNNNNCTT 2 cut(s) 753, 785
FatI CATG 7 cut(s) 255, 263, 396, 1015, 1153, 1264, 1307
FauNDI CATATG 1 cut(s) 437
FbaI TGATCA 1 cut(s) 1233
Fnu4HI GCNGC 3 cut(s) 117, 442, 703
FokI GGATG 1 cut(s) 240
Fsp4HI GCNGC 3 cut(s) 117, 442, 703
FspBI CTAG 4 cut(s) 183, 356, 816, 963
GluI GCNGC 3 cut(s) 117, 442, 703
GsaI CCCAGC 1 cut(s) 448
HaeIII GGCC 5 cut(s) 636, 659, 723, 1020, 1046
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 7 cut(s) 259, 267, 400, 1019, 1157, 1268, 1311
HincII GTYRAC 1 cut(s) 1272
HindII GTYRAC 1 cut(s) 1272
HinfI GANTC 3 cut(s) 260, 1089, 1148
HpaI GTTAAC 1 cut(s) 1272
HphI GGTGA 4 cut(s) 58, 359, 1169, 1286
Hpy166II GTNNAC 2 cut(s) 997, 1272
Hpy188I TCNGA 8 cut(s) 319, 472, 519, 551, 778, 790, 811, 834
Hpy188III TCNNGA 4 cut(s) 131, 225, 325, 1259
Hpy8I GTNNAC 2 cut(s) 997, 1272
HpyAV CCTTC 3 cut(s) 53, 264, 328
HpyCH4III ACNGT 1 cut(s) 1201
HpyCH4IV ACGT 5 cut(s) 24, 110, 321, 560, 1077
HpyCH4V TGCA 9 cut(s) 116, 218, 252, 589, 839, 908, 924, 973, 1085
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 633, 1063, 1175
HpyF3I CTNAG 5 cut(s) 469, 548, 787, 808, 1194
HpySE526I ACGT 5 cut(s) 24, 110, 321, 560, 1077
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 7 cut(s) 259, 267, 400, 1019, 1157, 1268, 1311
Ksp22I TGATCA 1 cut(s) 1233
KspAI GTTAAC 1 cut(s) 1272
LmnI GCTCC 5 cut(s) 210, 361, 857, 1054, 1223
Lsp1109I GCAGC 2 cut(s) 128, 689
LweI GCATC 3 cut(s) 73, 261, 373
MaeI CTAG 4 cut(s) 183, 356, 816, 963
MaeII ACGT 5 cut(s) 24, 110, 321, 560, 1077
MaeIII GTNAC 1 cut(s) 1073
MboII GAAGA 2 cut(s) 287, 1283
MflI RGATCY 4 cut(s) 127, 621, 804, 829
MhlI GDGCHC 1 cut(s) 854
MlsI TGGCCA 2 cut(s) 659, 723
MluCI AATT 5 cut(s) 678, 688, 780, 844, 1131
MluNI TGGCCA 2 cut(s) 659, 723
MmeI TCCRAC 4 cut(s) 118, 456, 542, 895
Mox20I TGGCCA 2 cut(s) 659, 723
Mph1103I ATGCAT 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 768
MscI TGGCCA 2 cut(s) 659, 723
MseI TTAA 7 cut(s) 104, 165, 237, 402, 1113, 1130, 1271
MslI CAYNNNNRTG 3 cut(s) 78, 200, 1306
Msp20I TGGCCA 2 cut(s) 659, 723
MspA1I CMGCKG 1 cut(s) 119
MspCI CTTAAG 1 cut(s) 401
MspR9I CCNGG 1 cut(s) 633
MvaI CCWGG 1 cut(s) 633
MwoI GCNNNNNNNGC 4 cut(s) 42, 633, 1063, 1175
NcoI CCATGG 1 cut(s) 1153
NdeI CATATG 1 cut(s) 437
NlaIII CATG 7 cut(s) 259, 267, 400, 1019, 1157, 1268, 1311
NlaIV GGNNCC 3 cut(s) 1045, 1056, 1219
NsiI ATGCAT 1 cut(s) 220
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 3 cut(s) 260, 1089, 1148
PkrI GCNGC 3 cut(s) 118, 443, 704
Ppu21I YACGTR 2 cut(s) 561, 1078
PshBI ATTAAT 1 cut(s) 1130
PsiI TTATAA 1 cut(s) 303
Psp6I CCWGG 1 cut(s) 631
PspFI CCCAGC 1 cut(s) 444
PspGI CCWGG 1 cut(s) 631
PspN4I GGNNCC 3 cut(s) 1045, 1056, 1219
PspPI GGNCC 3 cut(s) 629, 997, 1044
PsuI RGATCY 4 cut(s) 127, 621, 804, 829
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 1 cut(s) 1100
RsaNI GTAC 1 cut(s) 1099
RseI CAYNNNNRTG 3 cut(s) 78, 200, 1306
SaqAI TTAA 7 cut(s) 104, 165, 237, 402, 1113, 1130, 1271
SatI GCNGC 3 cut(s) 117, 442, 703
Sau96I GGNCC 3 cut(s) 629, 997, 1044
ScrFI CCNGG 1 cut(s) 633
SduI GDGCHC 1 cut(s) 854
SfaNI GCATC 3 cut(s) 73, 261, 373
SinI GGWCC 2 cut(s) 629, 997
SmiMI CAYNNNNRTG 3 cut(s) 78, 200, 1306
SmlI CTYRAG 2 cut(s) 401, 423
SmoI CTYRAG 2 cut(s) 401, 423
SnaBI TACGTA 1 cut(s) 1078
Sse9I AATT 5 cut(s) 678, 688, 780, 844, 1131
SsiI CCGC 2 cut(s) 441, 627
SspI AATATT 1 cut(s) 297
SspMI CTAG 4 cut(s) 183, 356, 816, 963
StyD4I CCNGG 1 cut(s) 631
StyI CCWWGG 1 cut(s) 1153
TaaI ACNGT 1 cut(s) 1201
TaiI ACGT 5 cut(s) 27, 113, 324, 563, 1080
TaqI TCGA 2 cut(s) 309, 1254
TaqII GACCGA 1 cut(s) 923
TasI AATT 5 cut(s) 678, 688, 780, 844, 1131
TatI WGTACW 1 cut(s) 1098
TauI GCSGC 1 cut(s) 444
TfiI GAWTC 3 cut(s) 260, 1089, 1148
Tru1I TTAA 7 cut(s) 104, 165, 237, 402, 1113, 1130, 1271
Tru9I TTAA 7 cut(s) 104, 165, 237, 402, 1113, 1130, 1271
TscAI CASTG 3 cut(s) 915, 1171, 1206
TseI GCWGC 2 cut(s) 116, 702
TspDTI ATGAA 4 cut(s) 255, 582, 743, 994
TspRI CASTG 3 cut(s) 915, 1171, 1206
Vha464I CTTAAG 1 cut(s) 401
VpaK11BI GGWCC 2 cut(s) 629, 997
VspI ATTAAT 1 cut(s) 1130
XapI RAATTY 4 cut(s) 678, 688, 780, 844
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 4 cut(s) 183, 356, 816, 963
ZraI GACGTC 1 cut(s) 322
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.