Rorug02G0539800

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0018067
Physical Location & Seq
Forward (+)
66998188 .. 66999118
931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0539800.1

Sequence Viewer

Length: 729 bp
ATGGTGAGAACTCCTTGCTGTGATGAAAGGGGAATGAAGAAAGGTACATGGACTCCGGAGGAAGATAGGAAGCTCATAGCTTATGTTACTAGGTATGGCTGCTGGAATTGGCGCCAACTCCCCAAGTTTGCTGGGCTTTCAAGGTGTGGGAAGAGTTGCAGGCTGAGGTGGCTGAATTACTTGAGGCCGAACATCAAGAGAGGAAACTACACCAATGAAGAAGACGAAATAATAATCAGGCTTCACGAAAAACTGGGGAACAAATGGTCTGCGATTGCGGCACAGCTTCAGGGAAGAACTGACAATGAGGTTAAGAATCACTGGCACACAAACCTCAAGAAACGTTTAATGATGAACAAGCACAAGGCGTCCTCAGTTTCAATCACACACGATGATCATCATGAAGAAGTAGTACCGAAATCATCGGATCAGCCAAAGACTACTGCTACTGCTGATTTCCTAAATGCAGAAACTACTGTTGATCAAATTCCCCAAATTGATTATTTTGATTACCCAGAACTGTGGTCACCACAGCCATCATCGAGTGAGGTCTCGTCCATGACCACCGATACTGCGGTTGCGATTACTGCCGCAGATGGTTTGGTTGTAGAGGAAAATGCTACTTCAGCTGCCATGGAAACAATTTATGGAGATTTCGGATATAATTTCTGGACAGAGCCATTTCTGGCTGATAATTCCTACAACCCAACTGGTTTTTATACACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

242

Amino Acids

27.72

Weight (kDa)

5.99

Isoelectric Point (pI)

41.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 2.2e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 2.2e-15 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 67 - 112 1.8e-18 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 114 9.3e-11 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49490 FvH4_6g49490 FvH4_6g49490 FvH4_6g49490 FvH4_6g49490 FvH4_6g49490
rosa_laevigata RLG00000021861
rosa_multiflora Rmu_sc0000648.1_g000013
rosa_roxburghii Rroxscaffold_2G00082150
rosa_rugosa Rorug02G0539800
rosa_wichuraiana Rw2G050710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 111
AccIII TCCGGA 1 cut(s) 55
AciI CCGC 3 cut(s) 278, 575, 591
AclI AACGTT 1 cut(s) 343
AclWI GGATC 1 cut(s) 435
AcsI RAATTY 1 cut(s) 486
AcuI CTGAAG 2 cut(s) 272, 609
AcyI GRCGYC 2 cut(s) 112, 368
AfaI GTAC 2 cut(s) 46, 414
AgsI TTSAA 2 cut(s) 141, 381
AluBI AGCT 4 cut(s) 73, 80, 286, 629
AluI AGCT 4 cut(s) 73, 80, 286, 629
Alw26I GTCTC 1 cut(s) 556
AlwI GGATC 1 cut(s) 435
Aor13HI TCCGGA 1 cut(s) 55
AoxI GGCC 1 cut(s) 185
ApeKI GCWGC 2 cut(s) 99, 629
ApoI RAATTY 1 cut(s) 486
AspLEI GCGC 1 cut(s) 114
AsuHPI GGTGA 2 cut(s) 16, 519
BaeI ACNNNNGTAYC 2 cut(s) 561, 594
BanI GGYRCC 1 cut(s) 111
BarI GAAGNNNNNNTAC 2 cut(s) 396, 428
BbsI GAAGAC 1 cut(s) 228
BbvCI CCTCAGC 1 cut(s) 164
BbvI GCAGC 2 cut(s) 86, 616
BccI CCATC 2 cut(s) 544, 590
BclI TGATCA 2 cut(s) 394, 481
BcoDI GTCTC 1 cut(s) 556
BfaI CTAG 2 cut(s) 90, 727
BfoI RGCGCY 1 cut(s) 115
BisI GCNGC 4 cut(s) 100, 279, 591, 630
BlsI GCNGC 4 cut(s) 101, 280, 592, 631
BmiI GGNNCC 1 cut(s) 113
BmrI ACTGGG 1 cut(s) 263
BmuI ACTGGG 1 cut(s) 263
BpiI GAAGAC 1 cut(s) 228
Bpu10I CCTNAGC 1 cut(s) 164
BpuEI CTTGAG 2 cut(s) 202, 320
BsaBI GATNNNNATC 1 cut(s) 396
BsaHI GRCGYC 2 cut(s) 112, 368
BsaI GGTCTC 1 cut(s) 556
BsaJI CCNNGG 1 cut(s) 633
BsaWI WCCGGW 1 cut(s) 55
BsaXI ACNNNNNCTCC 2 cut(s) 37, 67
Bse1I ACTGG 3 cut(s) 258, 326, 715
Bse8I GATNNNNATC 1 cut(s) 396
BseAI TCCGGA 1 cut(s) 55
BseDI CCNNGG 1 cut(s) 633
BseJI GATNNNNATC 1 cut(s) 396
BseMII CTCAG 2 cut(s) 155, 387
BseNI ACTGG 3 cut(s) 258, 326, 715
BseXI GCAGC 2 cut(s) 86, 616
BseYI CCCAGC 1 cut(s) 131
BshFI GGCC 1 cut(s) 187
BshNI GGYRCC 1 cut(s) 111
BsiSI CCGG 1 cut(s) 56
BsmAI GTCTC 1 cut(s) 556
BsnI GGCC 1 cut(s) 187
Bso31I GGTCTC 1 cut(s) 556
Bsp13I TCCGGA 1 cut(s) 55
Bsp143I GATC 3 cut(s) 394, 427, 481
Bsp19I CCATGG 1 cut(s) 633
BspACI CCGC 3 cut(s) 278, 575, 591
BspANI GGCC 1 cut(s) 187
BspCNI CTCAG 2 cut(s) 156, 386
BspEI TCCGGA 1 cut(s) 55
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 1 cut(s) 113
BspPI GGATC 1 cut(s) 435
BspT107I GGYRCC 1 cut(s) 111
BspTNI GGTCTC 1 cut(s) 556
BsrI ACTGG 3 cut(s) 258, 326, 715
BssECI CCNNGG 1 cut(s) 633
BssMI GATC 3 cut(s) 394, 427, 481
BssNI GRCGYC 2 cut(s) 112, 368
BssT1I CCWWGG 1 cut(s) 633
Bst4CI ACNGT 2 cut(s) 478, 522
Bst6I CTCTTC 1 cut(s) 146
BstACI GRCGYC 2 cut(s) 112, 368
BstC8I GCNNGC 1 cut(s) 161
BstDEI CTNAG 2 cut(s) 164, 373
BstDSI CCRYGG 1 cut(s) 633
BstEII GGTNACC 1 cut(s) 525
BstH2I RGCGCY 1 cut(s) 115
BstHHI GCGC 1 cut(s) 114
BstKTI GATC 3 cut(s) 397, 430, 484
BstMAI GTCTC 1 cut(s) 556
BstMBI GATC 3 cut(s) 394, 427, 481
BstMWI GCNNNNNNNGC 4 cut(s) 169, 278, 587, 626
BstPI GGTNACC 1 cut(s) 525
BstV1I GCAGC 2 cut(s) 86, 616
BstV2I GAAGAC 1 cut(s) 228
BstXI CCANNNNNNTGG 1 cut(s) 522
BsuRI GGCC 1 cut(s) 187
BtgI CCRYGG 1 cut(s) 633
BtsIMutI CAGTG 1 cut(s) 319
Cac8I GCNNGC 1 cut(s) 161
CciI TCATGA 1 cut(s) 400
CfoI GCGC 1 cut(s) 114
CseI GACGC 1 cut(s) 357
Csp6I GTAC 2 cut(s) 45, 413
CviAII CATG 4 cut(s) 48, 401, 559, 634
CviQI GTAC 2 cut(s) 45, 413
DdeI CTNAG 2 cut(s) 164, 373
DinI GGCGCC 1 cut(s) 113
DpnI GATC 3 cut(s) 396, 429, 483
DpnII GATC 3 cut(s) 394, 427, 481
Eam1104I CTCTTC 1 cut(s) 146
EarI CTCTTC 1 cut(s) 146
Eco130I CCWWGG 1 cut(s) 633
Eco31I GGTCTC 1 cut(s) 556
Eco57I CTGAAG 2 cut(s) 272, 609
Eco91I GGTNACC 1 cut(s) 525
EcoO65I GGTNACC 1 cut(s) 525
EcoT14I CCWWGG 1 cut(s) 633
EgeI GGCGCC 1 cut(s) 113
EheI GGCGCC 1 cut(s) 113
ErhI CCWWGG 1 cut(s) 633
FaeI CATG 4 cut(s) 51, 404, 562, 637
FatI CATG 4 cut(s) 47, 400, 558, 633
FbaI TGATCA 2 cut(s) 394, 481
Fnu4HI GCNGC 4 cut(s) 100, 279, 591, 630
Fsp4HI GCNGC 4 cut(s) 100, 279, 591, 630
FspBI CTAG 2 cut(s) 90, 727
GlaI GCGC 1 cut(s) 113
GluI GCNGC 4 cut(s) 100, 279, 591, 630
GsaI CCCAGC 1 cut(s) 135
HaeII RGCGCY 1 cut(s) 115
HaeIII GGCC 1 cut(s) 187
HapII CCGG 1 cut(s) 56
HgaI GACGC 1 cut(s) 357
HhaI GCGC 1 cut(s) 114
Hin1I GRCGYC 2 cut(s) 112, 368
Hin1II CATG 4 cut(s) 51, 404, 562, 637
Hin6I GCGC 1 cut(s) 112
HinP1I GCGC 1 cut(s) 112
HinfI GANTC 2 cut(s) 52, 316
HpaII CCGG 1 cut(s) 56
HphI GGTGA 2 cut(s) 16, 519
Hpy188I TCNGA 2 cut(s) 427, 659
Hpy188III TCNNGA 6 cut(s) 56, 196, 245, 337, 401, 670
HpyCH4III ACNGT 2 cut(s) 478, 522
HpyCH4IV ACGT 1 cut(s) 343
HpyCH4V TGCA 2 cut(s) 159, 467
HpyF10VI GCNNNNNNNGC 4 cut(s) 169, 278, 587, 626
HpyF3I CTNAG 2 cut(s) 164, 373
HpySE526I ACGT 1 cut(s) 343
Hsp92I GRCGYC 2 cut(s) 112, 368
Hsp92II CATG 4 cut(s) 51, 404, 562, 637
HspAI GCGC 1 cut(s) 112
KasI GGCGCC 1 cut(s) 111
Kpn2I TCCGGA 1 cut(s) 55
Ksp22I TGATCA 2 cut(s) 394, 481
Kzo9I GATC 3 cut(s) 394, 427, 481
Lsp1109I GCAGC 2 cut(s) 86, 616
MaeI CTAG 2 cut(s) 90, 727
MaeII ACGT 1 cut(s) 343
MaeIII GTNAC 2 cut(s) 85, 525
MalI GATC 3 cut(s) 396, 429, 483
MboI GATC 3 cut(s) 394, 427, 481
MboII GAAGA 7 cut(s) 49, 74, 163, 230, 233, 306, 416
MluCI AATT 7 cut(s) 106, 175, 486, 495, 642, 664, 694
Mly113I GGCGCC 1 cut(s) 112
MlyI GAGTC 1 cut(s) 46
MnlI CCTC 9 cut(s) 52, 159, 177, 194, 301, 344, 382, 541, 604
MroI TCCGGA 1 cut(s) 55
MseI TTAA 2 cut(s) 312, 347
MspA1I CMGCKG 1 cut(s) 629
MspI CCGG 1 cut(s) 56
MwoI GCNNNNNNNGC 4 cut(s) 169, 278, 587, 626
NarI GGCGCC 1 cut(s) 112
NcoI CCATGG 1 cut(s) 633
NdeII GATC 3 cut(s) 394, 427, 481
NlaIII CATG 4 cut(s) 51, 404, 562, 637
NlaIV GGNNCC 1 cut(s) 113
NmuCI GTSAC 1 cut(s) 525
PagI TCATGA 1 cut(s) 400
PfeI GAWTC 1 cut(s) 316
PkrI GCNGC 4 cut(s) 101, 280, 592, 631
PleI GAGTC 1 cut(s) 46
PluTI GGCGCC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 46
Psp1406I AACGTT 1 cut(s) 343
PspEI GGTNACC 1 cut(s) 525
PspFI CCCAGC 1 cut(s) 131
PspN4I GGNNCC 1 cut(s) 113
PvuII CAGCTG 1 cut(s) 629
RsaI GTAC 2 cut(s) 46, 414
RsaNI GTAC 2 cut(s) 45, 413
SaqAI TTAA 2 cut(s) 312, 347
SatI GCNGC 4 cut(s) 100, 279, 591, 630
Sau3AI GATC 3 cut(s) 394, 427, 481
SchI GAGTC 1 cut(s) 46
SfoI GGCGCC 1 cut(s) 113
SmlI CTYRAG 2 cut(s) 181, 335
SmoI CTYRAG 2 cut(s) 181, 335
Sse9I AATT 7 cut(s) 106, 175, 486, 495, 642, 664, 694
SsiI CCGC 3 cut(s) 278, 575, 591
SspDI GGCGCC 1 cut(s) 111
SspMI CTAG 2 cut(s) 90, 727
StyI CCWWGG 1 cut(s) 633
TaaI ACNGT 2 cut(s) 478, 522
TaiI ACGT 1 cut(s) 346
TaqI TCGA 1 cut(s) 542
TasI AATT 7 cut(s) 106, 175, 486, 495, 642, 664, 694
TauI GCSGC 2 cut(s) 281, 593
TfiI GAWTC 1 cut(s) 316
Tru1I TTAA 2 cut(s) 312, 347
Tru9I TTAA 2 cut(s) 312, 347
TscAI CASTG 1 cut(s) 326
TseFI GTSAC 1 cut(s) 525
TseI GCWGC 2 cut(s) 99, 629
Tsp45I GTSAC 1 cut(s) 525
TspDTI ATGAA 5 cut(s) 39, 50, 231, 368, 417
TspRI CASTG 1 cut(s) 326
XapI RAATTY 1 cut(s) 486
XspI CTAG 2 cut(s) 90, 727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.