RLG00000022281
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018820
Physical Location & Seq
Forward (+)
82220458 .. 82226017
5560 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022281

Sequence Viewer

Length: 1230 bp
ATGAGATCCGGTACCCGTCTTGGTGAACACACCCAAGATCGCCCACCCCAACCCACCCCCATGCGAATGCGAGTGGCCGATCTCACTACCGAGCAAAGGTGTATCTTGGTTGACCATCCGGAAAGTCTCCACCTTCAAATGGATCGAAGCCTGCAAGTAGACGAAGAACGGATCTTTATGCATCACCGCAACCCAACGGCGCCACCAAAGATCCTGTACATAGCCAAATACGGTGCCCAGGTTGAAACTTCCGTCGAAGAGCTCTCATCCGTTGATCAACCTGACCATCGTTTTGGGTTTTCAACAGACGCCGTATATGGCTTTGGGCTTGCAAATTTTGGGGGCTTCAAGATTCTGGATATGCCATATCCACCGATGATCGTGCTGTCTTTGTTCCGAAGCATGGCTTACTGTTCTGGAAAGCTTATGTTAATCATAAAGATGTTCTGGTTGGAAGAAGCGGTAGTCTTGGACGACGAGATCGACGTTTGGGACGAAGGCTCTGAGGTCGTGGGATTCGATGTCGAGAGCGAAGCAGGAGAGGCCATTATGGGCGAGGAAGATGGCGGTGGCCTGAAAAGTCCAGCTGATGTCGTTGCCATAGCCGTGGACCATGAAGATGAGGCCACGTGGAGGAGAGACAAGGGGAAGCCATTGGCTAGTGAAGAGTTTGAGGCCTCTGGGAGAGGTGAACAATATATTTTCTTCCTCTGCAATGACAACAGCATTGTTGGATTCAAGTTTCGGAACCATGATGGTGTTTTATGGAAACCAACAGTGAAGTTCTCTATGGATCTGTTTCACCTTGGTCTTGCTATGGGAATTGATGGTCTCCAATTTCCTAAATACAAAGCAATAGGTTTAGGACCAAAGGAAATCCAACTGGACAAAGAGCAGGAACTCCTAAAATTCCCAAAACTCGATGGCCCCACAATCATCGTCAGCCTTGCGGCTCTCTGTTTCTTGCTGATTGCTGTGTTTTATTGTTTCTATGGGAAGCCTTCTTATTCTCCTTTTGTCGTCAACCAAGTGCTCCGATTAGAGGATGATGTCATGGTGGACGTCTTGGTGGCTGGAATATCCGTTGACCGTCCGCTCTGGTTGGGAGCTAGGACTCGAAAACTATATCTAAGTTCAGGTCGAAGCTTCACGGGTGATCCGATGGCTGTTTGTTCTGTTGGGTTTCCGTCAGATGCTGTTGTTGGACTTCGAAGGGGTGGTCAAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

410

Amino Acids

45.57

Weight (kDa)

4.97

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018820)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24731 FvH4_6g52620 FvH4_6g52620 FvH4_6g52620
prunus_persica Prupe.8G121800_v2.0.a1
rosa_laevigata RLG00000022281
rosa_roxburghii Rroxscaffold_1G00066580 Rroxscaffold_6G00419070
rosa_samantha Rh5BG261400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1065
Acc65I GGTACC 1 cut(s) 11
AccB1I GGYRCC 3 cut(s) 11, 199, 233
AccBSI CCGCTC 1 cut(s) 1096
AccI GTMKAC 1 cut(s) 159
AccIII TCCGGA 1 cut(s) 118
AciI CCGC 5 cut(s) 187, 461, 567, 950, 1094
AclWI GGATC 5 cut(s) 150, 179, 205, 801, 1151
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 2 cut(s) 334, 908
AcvI CACGTG 1 cut(s) 630
AcyI GRCGYC 3 cut(s) 200, 309, 1062
AfaI GTAC 2 cut(s) 13, 218
AfiI CCNNNNNNNGG 5 cut(s) 96, 139, 403, 633, 1042
AgsI TTSAA 5 cut(s) 137, 245, 303, 349, 739
AjnI CCWGG 1 cut(s) 237
AluBI AGCT 5 cut(s) 262, 424, 587, 1109, 1146
AluI AGCT 5 cut(s) 262, 424, 587, 1109, 1146
Alw21I GWGCWC 2 cut(s) 264, 1035
Alw26I GTCTC 3 cut(s) 131, 633, 836
AlwI GGATC 5 cut(s) 150, 179, 205, 801, 1151
AlwNI CAGNNNCTG 1 cut(s) 1196
Aor13HI TCCGGA 1 cut(s) 118
AoxI GGCC 6 cut(s) 75, 543, 571, 624, 675, 925
ApoI RAATTY 2 cut(s) 334, 908
ArsI GACNNNNNNTTYG 3 cut(s) 275, 307, 1204
Asp718I GGTACC 1 cut(s) 11
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 3 cut(s) 610, 866, 926
AsuHPI GGTGA 5 cut(s) 35, 176, 701, 794, 1166
AsuII TTCGAA 1 cut(s) 1210
AvaII GGWCC 2 cut(s) 610, 866
BaeGI GKGCMC 1 cut(s) 238
BanI GGYRCC 3 cut(s) 11, 199, 233
BanII GRGCYC 1 cut(s) 264
BbrPI CACGTG 1 cut(s) 630
Bbv12I GWGCWC 2 cut(s) 264, 1035
BccI CCATC 7 cut(s) 123, 294, 557, 749, 821, 917, 1156
BceAI ACGGC 3 cut(s) 213, 296, 590
BcgI CGANNNNNNTGC 2 cut(s) 364, 398
BciT130I CCWGG 1 cut(s) 239
BclI TGATCA 1 cut(s) 274
BcoDI GTCTC 3 cut(s) 131, 633, 836
BfaI CTAG 2 cut(s) 660, 1110
BfoI RGCGCY 1 cut(s) 203
BisI GCNGC 1 cut(s) 951
BlsI GCNGC 1 cut(s) 952
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 2 cut(s) 610, 866
BmgT120I GGNCC 3 cut(s) 610, 866, 926
BmiI GGNNCC 5 cut(s) 13, 201, 235, 749, 928
BmrFI CCNGG 1 cut(s) 239
BmsI GCATC 2 cut(s) 190, 1183
Bpu14I TTCGAA 1 cut(s) 1210
BsaAI YACGTR 1 cut(s) 630
BsaHI GRCGYC 3 cut(s) 200, 309, 1062
BsaI GGTCTC 1 cut(s) 836
BsaJI CCNNGG 3 cut(s) 237, 606, 805
BsaWI WCCGGW 2 cut(s) 8, 118
Bsc4I CCNNNNNNNGG 5 cut(s) 96, 139, 403, 633, 1042
Bse1I ACTGG 1 cut(s) 888
Bse3DI GCAATG 1 cut(s) 721
BseAI TCCGGA 1 cut(s) 118
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 3 cut(s) 237, 606, 805
BseGI GGATG 3 cut(s) 115, 266, 1051
BseLI CCNNNNNNNGG 5 cut(s) 96, 139, 403, 633, 1042
BseMI GCAATG 1 cut(s) 721
BseMII CTCAG 1 cut(s) 495
BseNI ACTGG 1 cut(s) 888
BseRI GAGGAG 1 cut(s) 649
BseSI GKGCMC 1 cut(s) 238
BshFI GGCC 6 cut(s) 77, 545, 573, 626, 677, 927
BshNI GGYRCC 3 cut(s) 11, 199, 233
BsiHKAI GWGCWC 2 cut(s) 264, 1035
BsiSI CCGG 2 cut(s) 9, 119
BslFI GGGAC 1 cut(s) 506
BslI CCNNNNNNNGG 5 cut(s) 96, 139, 403, 633, 1042
BsmAI GTCTC 3 cut(s) 131, 633, 836
BsmFI GGGAC 1 cut(s) 506
BsmI GAATGC 1 cut(s) 72
BsnI GGCC 6 cut(s) 77, 545, 573, 626, 677, 927
Bso31I GGTCTC 1 cut(s) 836
Bsp119I TTCGAA 1 cut(s) 1210
Bsp1286I GDGCHC 3 cut(s) 238, 264, 1035
Bsp13I TCCGGA 1 cut(s) 118
Bsp1407I TGTACA 1 cut(s) 216
BspACI CCGC 5 cut(s) 187, 461, 567, 950, 1094
BspANI GGCC 6 cut(s) 77, 545, 573, 626, 677, 927
BspCNI CTCAG 1 cut(s) 496
BspEI TCCGGA 1 cut(s) 118
BspLI GGNNCC 5 cut(s) 13, 201, 235, 749, 928
BspPI GGATC 5 cut(s) 150, 179, 205, 801, 1151
BspQI GCTCTTC 1 cut(s) 252
BspT104I TTCGAA 1 cut(s) 1210
BspT107I GGYRCC 3 cut(s) 11, 199, 233
BspTNI GGTCTC 1 cut(s) 836
BsrBI CCGCTC 1 cut(s) 1096
BsrDI GCAATG 1 cut(s) 721
BsrGI TGTACA 1 cut(s) 216
BsrI ACTGG 1 cut(s) 888
BssECI CCNNGG 3 cut(s) 237, 606, 805
BssNI GRCGYC 3 cut(s) 200, 309, 1062
BssT1I CCWWGG 1 cut(s) 805
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 4 cut(s) 233, 413, 778, 1091
Bst6I CTCTTC 2 cut(s) 252, 660
BstACI GRCGYC 3 cut(s) 200, 309, 1062
BstAUI TGTACA 1 cut(s) 216
BstBAI YACGTR 1 cut(s) 630
BstBI TTCGAA 1 cut(s) 1210
BstC8I GCNNGC 2 cut(s) 152, 330
BstDEI CTNAG 2 cut(s) 504, 1130
BstDSI CCRYGG 1 cut(s) 606
BstF5I GGATG 3 cut(s) 115, 266, 1051
BstH2I RGCGCY 1 cut(s) 203
BstHHI GCGC 1 cut(s) 202
BstMAI GTCTC 3 cut(s) 131, 633, 836
BstMWI GCNNNNNNNGC 1 cut(s) 542
BstNI CCWGG 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 237
BstSLI GKGCMC 1 cut(s) 238
BstX2I RGATCY 4 cut(s) 5, 171, 210, 793
BstXI CCANNNNNNTGG 2 cut(s) 293, 607
BstYI RGATCY 4 cut(s) 5, 171, 210, 793
BsuRI GGCC 6 cut(s) 77, 545, 573, 626, 677, 927
BtgI CCRYGG 1 cut(s) 606
BtsCI GGATG 3 cut(s) 115, 266, 1051
BtsIMutI CAGTG 1 cut(s) 783
Cac8I GCNNGC 2 cut(s) 152, 330
CaiI CAGNNNCTG 1 cut(s) 1196
CfoI GCGC 1 cut(s) 202
Cfr13I GGNCC 3 cut(s) 610, 866, 926
CseI GACGC 1 cut(s) 317
Csp6I GTAC 2 cut(s) 12, 217
CviAII CATG 5 cut(s) 61, 403, 614, 752, 1054
CviQI GTAC 2 cut(s) 12, 217
DdeI CTNAG 2 cut(s) 504, 1130
DinI GGCGCC 1 cut(s) 201
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 2 cut(s) 252, 660
EarI CTCTTC 2 cut(s) 252, 660
Ecl136II GAGCTC 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 805
Eco147I AGGCCT 1 cut(s) 677
Eco24I GRGCYC 1 cut(s) 264
Eco31I GGTCTC 1 cut(s) 836
Eco47I GGWCC 2 cut(s) 610, 866
Eco53kI GAGCTC 1 cut(s) 262
Eco72I CACGTG 1 cut(s) 630
EcoICRI GAGCTC 1 cut(s) 262
EcoRII CCWGG 1 cut(s) 237
EcoT14I CCWWGG 1 cut(s) 805
EcoT22I ATGCAT 1 cut(s) 183
EcoT38I GRGCYC 1 cut(s) 264
EgeI GGCGCC 1 cut(s) 201
EheI GGCGCC 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 805
FaeI CATG 5 cut(s) 64, 406, 617, 755, 1057
FalI AAGNNNNNCTT 2 cut(s) 391, 423
FaqI GGGAC 1 cut(s) 506
FatI CATG 5 cut(s) 60, 402, 613, 751, 1053
FbaI TGATCA 1 cut(s) 274
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 951
FokI GGATG 3 cut(s) 102, 253, 1058
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 1 cut(s) 951
FspBI CTAG 2 cut(s) 660, 1110
GlaI GCGC 1 cut(s) 201
GluI GCNGC 1 cut(s) 951
HaeII RGCGCY 1 cut(s) 203
HaeIII GGCC 6 cut(s) 77, 545, 573, 626, 677, 927
HapII CCGG 2 cut(s) 9, 119
HgaI GACGC 1 cut(s) 317
HhaI GCGC 1 cut(s) 202
Hin1I GRCGYC 3 cut(s) 200, 309, 1062
Hin1II CATG 5 cut(s) 64, 406, 617, 755, 1057
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HincII GTYRAC 3 cut(s) 112, 1024, 1087
HindII GTYRAC 3 cut(s) 112, 1024, 1087
HindIII AAGCTT 2 cut(s) 422, 1144
HinfI GANTC 4 cut(s) 352, 516, 735, 1114
HpaII CCGG 2 cut(s) 9, 119
HphI GGTGA 5 cut(s) 35, 176, 701, 794, 1166
Hpy166II GTNNAC 8 cut(s) 26, 112, 160, 610, 692, 1024, 1060, 1087
Hpy188I TCNGA 6 cut(s) 398, 505, 747, 1037, 1161, 1192
Hpy188III TCNNGA 5 cut(s) 119, 349, 356, 417, 526
Hpy8I GTNNAC 8 cut(s) 26, 112, 160, 610, 692, 1024, 1060, 1087
Hpy99I CGWCG 3 cut(s) 257, 479, 488
HpyAV CCTTC 4 cut(s) 143, 491, 1011, 1206
HpyCH4III ACNGT 4 cut(s) 233, 413, 778, 1091
HpyCH4IV ACGT 3 cut(s) 486, 629, 1062
HpyCH4V TGCA 4 cut(s) 154, 181, 332, 714
HpyF10VI GCNNNNNNNGC 1 cut(s) 542
HpyF3I CTNAG 2 cut(s) 504, 1130
HpySE526I ACGT 3 cut(s) 486, 629, 1062
Hsp92I GRCGYC 3 cut(s) 200, 309, 1062
Hsp92II CATG 5 cut(s) 64, 406, 617, 755, 1057
HspAI GCGC 1 cut(s) 200
KasI GGCGCC 1 cut(s) 199
Kpn2I TCCGGA 1 cut(s) 118
KpnI GGTACC 1 cut(s) 15
Ksp22I TGATCA 1 cut(s) 274
LguI GCTCTTC 1 cut(s) 252
LmnI GCTCC 2 cut(s) 1038, 1106
LweI GCATC 2 cut(s) 190, 1183
MaeI CTAG 2 cut(s) 660, 1110
MaeII ACGT 3 cut(s) 486, 629, 1062
MbiI CCGCTC 1 cut(s) 1096
MboII GAAGA 7 cut(s) 176, 269, 467, 572, 629, 677, 697
MflI RGATCY 4 cut(s) 5, 171, 210, 793
MhlI GDGCHC 3 cut(s) 238, 264, 1035
MluCI AATT 4 cut(s) 334, 822, 836, 908
Mly113I GGCGCC 1 cut(s) 200
MlyI GAGTC 1 cut(s) 1108
MmeI TCCRAC 4 cut(s) 432, 712, 904, 1183
Mph1103I ATGCAT 1 cut(s) 183
MroI TCCGGA 1 cut(s) 118
MseI TTAA 1 cut(s) 431
MslI CAYNNNNRTG 6 cut(s) 59, 65, 440, 605, 618, 756
MspA1I CMGCKG 1 cut(s) 587
MspI CCGG 2 cut(s) 9, 119
MspR9I CCNGG 1 cut(s) 239
Mva1269I GAATGC 1 cut(s) 72
MvaI CCWGG 1 cut(s) 239
MwoI GCNNNNNNNGC 1 cut(s) 542
NarI GGCGCC 1 cut(s) 200
NlaIII CATG 5 cut(s) 64, 406, 617, 755, 1057
NlaIV GGNNCC 5 cut(s) 13, 201, 235, 749, 928
NsiI ATGCAT 1 cut(s) 183
NspV TTCGAA 1 cut(s) 1210
PceI AGGCCT 1 cut(s) 677
PciSI GCTCTTC 1 cut(s) 252
PcsI WCGNNNNNNNCGW 5 cut(s) 480, 483, 492, 516, 1157
PctI GAATGC 1 cut(s) 72
PfeI GAWTC 3 cut(s) 352, 516, 735
PkrI GCNGC 1 cut(s) 952
PleI GAGTC 1 cut(s) 1108
PluTI GGCGCC 1 cut(s) 203
PmaCI CACGTG 1 cut(s) 630
PmlI CACGTG 1 cut(s) 630
PpsI GAGTC 1 cut(s) 1108
Ppu21I YACGTR 1 cut(s) 630
Psp124BI GAGCTC 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 237
PspCI CACGTG 1 cut(s) 630
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 5 cut(s) 13, 201, 235, 749, 928
PspPI GGNCC 3 cut(s) 610, 866, 926
PstNI CAGNNNCTG 1 cut(s) 1196
PsuI RGATCY 4 cut(s) 5, 171, 210, 793
PvuII CAGCTG 1 cut(s) 587
RsaI GTAC 2 cut(s) 13, 218
RsaNI GTAC 2 cut(s) 12, 217
RseI CAYNNNNRTG 6 cut(s) 59, 65, 440, 605, 618, 756
SacI GAGCTC 1 cut(s) 264
SapI GCTCTTC 1 cut(s) 252
SaqAI TTAA 1 cut(s) 431
SatI GCNGC 1 cut(s) 951
Sau96I GGNCC 3 cut(s) 610, 866, 926
SchI GAGTC 1 cut(s) 1108
ScrFI CCNGG 1 cut(s) 239
SduI GDGCHC 3 cut(s) 238, 264, 1035
SfaNI GCATC 2 cut(s) 190, 1183
SfoI GGCGCC 1 cut(s) 201
SfuI TTCGAA 1 cut(s) 1210
SinI GGWCC 2 cut(s) 610, 866
SmiMI CAYNNNNRTG 6 cut(s) 59, 65, 440, 605, 618, 756
Sse9I AATT 4 cut(s) 334, 822, 836, 908
SseBI AGGCCT 1 cut(s) 677
SsiI CCGC 5 cut(s) 187, 461, 567, 950, 1094
SspDI GGCGCC 1 cut(s) 199
SspMI CTAG 2 cut(s) 660, 1110
SstI GAGCTC 1 cut(s) 264
StuI AGGCCT 1 cut(s) 677
StyD4I CCNGG 1 cut(s) 237
StyI CCWWGG 1 cut(s) 805
TaaI ACNGT 4 cut(s) 233, 413, 778, 1091
TaiI ACGT 3 cut(s) 489, 632, 1065
TaqI TCGA 9 cut(s) 145, 255, 483, 519, 525, 921, 1117, 1141, 1210
TasI AATT 4 cut(s) 334, 822, 836, 908
TatI WGTACW 1 cut(s) 216
TauI GCSGC 1 cut(s) 953
TfiI GAWTC 3 cut(s) 352, 516, 735
Tru1I TTAA 1 cut(s) 431
Tru9I TTAA 1 cut(s) 431
TscAI CASTG 1 cut(s) 783
TspDTI ATGAA 1 cut(s) 630
TspGWI ACGGA 5 cut(s) 184, 241, 259, 1072, 1176
TspRI CASTG 1 cut(s) 783
VpaK11BI GGWCC 2 cut(s) 610, 866
XapI RAATTY 2 cut(s) 334, 908
XmiI GTMKAC 1 cut(s) 159
XspI CTAG 2 cut(s) 660, 1110
ZraI GACGTC 1 cut(s) 1063
Zsp2I ATGCAT 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.