RLG00000023035

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017589
Physical Location & Seq
Reverse (-)
17003131 .. 17004958
1828 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023035

Sequence Viewer

Length: 1662 bp
ATGCGGAGGTTAGAGGGAACCATAGTTCTAACGACTTGCCGCCAGTTCTGTAAGCTATCACCGCCCAGTTTGAATCACATTCACATGCATGCAATTCCATTTTCTGATCGAAATCCCACTCCCGAAAACAACATTGTGTCATGGAACAAAATGATTGCTGGGTACGTCCGAAATGGCCAAATGCGAGTTGCCCACAACCTGTTCGATGAAATGCCTGTGAAAGACGAAGTTTCCTGGAACACCATCTTATCGGGCCTGCACAACACCAAAGACGCACATGGAGTTAATCGACGTTTCTTGCAAATGAGAAGAGATGGGTTTAGACCAAATGAGTATACCATCTCCATAGTATTGAGAGCCTTTTTGGGGACTGTGCTTAATGTTTTGGTCCCGCAGATTCATGCCTTGGCAATACTCTCGGCGCTCAACTCAACTGTGTTTGTTGGGTCGGCACTTATGAAAGGGTATGCAAATATAGGGGATGGAGTAGCTATGGCTAGAGCATTCGATGAGATTTCGGCCAAGGATGTGTCGTCTTGGAATGCATTGATTTCGAGTTACATGGAATTGGGGTGCATGCGTGACGCTCAAAGAGTTTTTGATGGGATGCCGGAGAGGAATATAGTTTCTTGGACTAGTTTAGTAAATGGGTATATCAGAAATAGGAGGGTTAACAAAGCTCGCTCCGTTTTCAATAAAATGAGTGCAAAAAATGTGGTCTCATGGACTGTTATGATCAGTGGTTATGCGAAGAATCAGAAATTTGTAGATGCTTTGGAACTTTTCCTCTTGATGTTGAAGTCAGGGACGCTGCCCAATCAGTTCACATTTTCAAGTGTGCTGGATGCAAGCGCTGGTTGTTCTTCACTTATCTTCGGTCAGCAAGTGCACTCACGCATCTTAAAGTCTGGTATACCAGAGGATGTAACCTTGTCAACCTCGCTTGTTGATATGTATGCAAAATGCGGTGATATTGCTGCAGCGTTTTGTATTTTTGGATCCATGCCAAAGAAAAACTTGATATCCTGGAATTCAATTATAGGAGGTTATGCAAGGCATGGATTTGCTACAAGAGCATTGGAGGAGTTTGAGAGAATGACCAACTGTGGTGTTAGGCCTGATGCAGTTACATTCACAAATGCGTTATCAGCTTGCGCACATGGTGGGATGATTAAAGAAGGAAAAAGCCTCTTTAACTCCATGAAGTCAAAGTTCGGAAAGGAACCACAGATGGAGCATTATGCTTGCATGGTGGACCTCTATGGGAAAGCAGGTGAGCTAGAGGAAGCGGAGAAGTTGATACATGGGATGCCATTTCAGCCGGATGTTGTTATTTGGTGTGCGTTACTTGGTGCTTGTGGTATGCATTCAAGTTTGGAACTTGGTGAGTTTGCTGCAGAGGAACTTGATAAACTAGAAAGTGACCATGCTGCAATATATTCAACGCTTTCAAGGATTCATGGGGAAAGAGGAGTTTGGAATAGTGTGCTCGAGTTAAGGAATACAATGAAAGAGAGAGGCGTTCAAAAGCAGAATGCAGGTAGCTGGGTTGAGTCTGCACGACCAAAGGAGTCACAATCGGAGTCAAAATCTCCCCCGACACCCTACTCTTATTGGTCTCCCAATGGAGAGATTCTCTTCGGGTACAATCTCAGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

554

Amino Acids

61.51

Weight (kDa)

8.73

Isoelectric Point (pI)

48.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 44 - 72 1e-07 PPR repeat family
PPR PF01535 46 - 72 2.9e-06 PPR repeat
PPR_2 PF13041 75 - 121 5.6e-09 PPR repeat family
PPR_2 PF13041 206 - 239 1.3e-06 PPR repeat family
PPR PF01535 209 - 238 2.3e-06 PPR repeat
PPR_2 PF13041 237 - 283 3.6e-12 PPR repeat family
PPR PF01535 240 - 269 2.9e-07 PPR repeat
PPR_2 PF13041 339 - 387 1.1e-08 PPR repeat family
E_motif PF20431 456 - 518 6.2e-10 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017589)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26830 FvH4_1g26830
malus_domestica MD17G1278700.v1.1
prunus_persica Prupe.3G007300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0487071
rosa_laevigata RLG00000023035
rosa_multiflora Rmu_sc0005856.1_g000034
rosa_roxburghii Rroxscaffold_6G00395200
rosa_samantha Rh3BG315000 Rh3CG313400 Rh3DG310000
rosa_wichuraiana Rw3G024740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1262
Acc16I TGCGCA 1 cut(s) 1156
Acc36I ACCTGC 2 cut(s) 1262, 1529
AccI GTMKAC 2 cut(s) 335, 913
AciI CCGC 6 cut(s) 4, 40, 62, 392, 966, 1289
AclWI GGATC 2 cut(s) 993, 1006
AcoI YGGCCR 2 cut(s) 175, 519
AcsI RAATTY 2 cut(s) 761, 1030
AdeI CACNNNGTG 1 cut(s) 1163
AfaI GTAC 2 cut(s) 164, 1646
AfeI AGCGCT 1 cut(s) 853
AfiI CCNNNNNNNGG 1 cut(s) 366
AgsI TTSAA 9 cut(s) 73, 694, 799, 834, 1035, 1371, 1443, 1452, 1526
AhlI ACTAGT 1 cut(s) 635
AjnI CCWGG 2 cut(s) 233, 1025
AloI GAACNNNNNNTCC 2 cut(s) 9, 41
AluBI AGCT 6 cut(s) 55, 491, 680, 1151, 1279, 1545
AluI AGCT 6 cut(s) 55, 491, 680, 1151, 1279, 1545
Alw21I GWGCWC 2 cut(s) 891, 1491
Alw26I GTCTC 2 cut(s) 724, 1623
Alw44I GTGCAC 1 cut(s) 887
AlwI GGATC 2 cut(s) 993, 1006
AlwNI CAGNNNCTG 1 cut(s) 1659
Ama87I CYCGRG 1 cut(s) 1490
Aor51HI AGCGCT 1 cut(s) 853
AoxI GGCC 4 cut(s) 175, 253, 519, 1115
ApaLI GTGCAC 1 cut(s) 887
ApeKI GCWGC 5 cut(s) 811, 977, 980, 1394, 1430
ApoI RAATTY 2 cut(s) 761, 1030
AspLEI GCGC 3 cut(s) 424, 854, 1157
AspS9I GGNCC 3 cut(s) 253, 388, 1255
AsuHPI GGTGA 4 cut(s) 51, 980, 1286, 1397
AvaI CYCGRG 1 cut(s) 1490
AvaII GGWCC 2 cut(s) 388, 1255
BaeGI GKGCMC 1 cut(s) 891
BalI TGGCCA 1 cut(s) 177
BamHI GGATCC 1 cut(s) 998
Bbv12I GWGCWC 2 cut(s) 891, 1491
BbvI GCAGC 5 cut(s) 798, 964, 992, 1381, 1417
BccI CCATC 6 cut(s) 251, 308, 347, 476, 596, 1225
BcgI CGANNNNNNTGC 4 cut(s) 399, 433, 534, 568
BciT130I CCWGG 2 cut(s) 235, 1027
BclI TGATCA 1 cut(s) 735
BcoDI GTCTC 2 cut(s) 724, 1623
BcuI ACTAGT 1 cut(s) 635
BfaI CTAG 4 cut(s) 498, 636, 1280, 1415
BfmI CTRYAG 2 cut(s) 978, 1395
BfoI RGCGCY 2 cut(s) 425, 855
BfuAI ACCTGC 2 cut(s) 1262, 1529
BisI GCNGC 6 cut(s) 40, 812, 978, 981, 1395, 1431
BlsI GCNGC 6 cut(s) 41, 813, 979, 982, 1396, 1432
Bme1390I CCNGG 2 cut(s) 235, 1027
Bme18I GGWCC 2 cut(s) 388, 1255
BmeT110I CYCGRG 1 cut(s) 1490
BmgT120I GGNCC 3 cut(s) 253, 388, 1255
BmiI GGNNCC 4 cut(s) 19, 390, 1000, 1224
BmrFI CCNGG 2 cut(s) 235, 1027
BmrI ACTGGG 1 cut(s) 60
BmsI GCATC 6 cut(s) 597, 760, 835, 906, 1111, 1299
BmuI ACTGGG 1 cut(s) 60
BplI GAGNNNNNCTC 2 cut(s) 1620, 1652
BsaBI GATNNNNATC 1 cut(s) 111
BsaI GGTCTC 2 cut(s) 724, 1623
BsaJI CCNNGG 2 cut(s) 405, 522
Bsc4I CCNNNNNNNGG 1 cut(s) 366
Bse1I ACTGG 2 cut(s) 43, 66
Bse8I GATNNNNATC 1 cut(s) 111
BseBI CCWGG 2 cut(s) 235, 1027
BseDI CCNNGG 2 cut(s) 405, 522
BseGI GGATG 8 cut(s) 487, 532, 612, 850, 928, 1173, 1314, 1330
BseJI GATNNNNATC 1 cut(s) 111
BseLI CCNNNNNNNGG 1 cut(s) 366
BseNI ACTGG 2 cut(s) 43, 66
BseRI GAGGAG 2 cut(s) 1097, 1485
BseSI GKGCMC 1 cut(s) 891
BseXI GCAGC 5 cut(s) 798, 964, 992, 1381, 1417
BseYI CCCAGC 2 cut(s) 158, 1545
BsgI GTGCAG 2 cut(s) 242, 1542
BshFI GGCC 4 cut(s) 177, 255, 521, 1117
BsiHKAI GWGCWC 2 cut(s) 891, 1491
BsiHKCI CYCGRG 1 cut(s) 1490
BsiSI CCGG 2 cut(s) 611, 1322
BslFI GGGAC 3 cut(s) 374, 382, 820
BslI CCNNNNNNNGG 1 cut(s) 366
BsmAI GTCTC 2 cut(s) 724, 1623
BsmFI GGGAC 3 cut(s) 374, 382, 820
BsmI GAATGC 4 cut(s) 503, 547, 1366, 1540
BsnI GGCC 4 cut(s) 177, 255, 521, 1117
Bso31I GGTCTC 2 cut(s) 724, 1623
BsoBI CYCGRG 1 cut(s) 1490
Bsp1286I GDGCHC 2 cut(s) 891, 1491
Bsp143I GATC 3 cut(s) 106, 735, 998
BspACI CCGC 6 cut(s) 4, 40, 62, 392, 966, 1289
BspANI GGCC 4 cut(s) 177, 255, 521, 1117
BspLI GGNNCC 4 cut(s) 19, 390, 1000, 1224
BspMAI CTGCAG 2 cut(s) 982, 1399
BspMI ACCTGC 2 cut(s) 1262, 1529
BspPI GGATC 2 cut(s) 993, 1006
BspTNI GGTCTC 2 cut(s) 724, 1623
BsrI ACTGG 2 cut(s) 43, 66
BssECI CCNNGG 2 cut(s) 405, 522
BssMI GATC 3 cut(s) 106, 735, 998
BssNAI GTATAC 2 cut(s) 336, 914
BssT1I CCWWGG 2 cut(s) 405, 522
Bst1107I GTATAC 2 cut(s) 336, 914
Bst2UI CCWGG 2 cut(s) 235, 1027
Bst4CI ACNGT 4 cut(s) 373, 436, 730, 1106
Bst6I CTCTTC 2 cut(s) 304, 1643
BstC8I GCNNGC 7 cut(s) 90, 257, 578, 682, 850, 1153, 1246
BstDEI CTNAG 1 cut(s) 1652
BstF5I GGATG 8 cut(s) 487, 532, 612, 850, 928, 1173, 1314, 1330
BstH2I RGCGCY 2 cut(s) 425, 855
BstHHI GCGC 3 cut(s) 424, 854, 1157
BstKTI GATC 3 cut(s) 109, 738, 1001
BstMAI GTCTC 2 cut(s) 724, 1623
BstMBI GATC 3 cut(s) 106, 735, 998
BstMWI GCNNNNNNNGC 4 cut(s) 61, 1073, 1148, 1318
BstNI CCWGG 2 cut(s) 235, 1027
BstNSI RCATGY 3 cut(s) 88, 92, 580
BstSCI CCNGG 2 cut(s) 233, 1025
BstSFI CTRYAG 2 cut(s) 978, 1395
BstSLI GKGCMC 1 cut(s) 891
BstV1I GCAGC 5 cut(s) 798, 964, 992, 1381, 1417
BstX2I RGATCY 1 cut(s) 998
BstYI RGATCY 1 cut(s) 998
BstZ17I GTATAC 2 cut(s) 336, 914
BsuRI GGCC 4 cut(s) 177, 255, 521, 1117
BtsCI GGATG 8 cut(s) 487, 532, 612, 850, 928, 1173, 1314, 1330
BtsIMutI CAGTG 1 cut(s) 745
BveI ACCTGC 2 cut(s) 1262, 1529
Cac8I GCNNGC 7 cut(s) 90, 257, 578, 682, 850, 1153, 1246
CaiI CAGNNNCTG 1 cut(s) 1659
CfoI GCGC 3 cut(s) 424, 854, 1157
Cfr13I GGNCC 3 cut(s) 253, 388, 1255
CseI GACGC 3 cut(s) 281, 593, 817
Csp6I GTAC 2 cut(s) 163, 1645
CspCI CAANNNNNGTGG 2 cut(s) 696, 731
CviQI GTAC 2 cut(s) 163, 1645
DdeI CTNAG 1 cut(s) 1652
DpnI GATC 3 cut(s) 108, 737, 1000
DpnII GATC 3 cut(s) 106, 735, 998
DraIII CACNNNGTG 1 cut(s) 1163
EaeI YGGCCR 2 cut(s) 175, 519
Eam1104I CTCTTC 2 cut(s) 304, 1643
EarI CTCTTC 2 cut(s) 304, 1643
Eco130I CCWWGG 2 cut(s) 405, 522
Eco147I AGGCCT 1 cut(s) 1117
Eco31I GGTCTC 2 cut(s) 724, 1623
Eco32I GATATC 1 cut(s) 1023
Eco47I GGWCC 2 cut(s) 388, 1255
Eco47III AGCGCT 1 cut(s) 853
Eco88I CYCGRG 1 cut(s) 1490
EcoRI GAATTC 1 cut(s) 1030
EcoRII CCWGG 2 cut(s) 233, 1025
EcoRV GATATC 1 cut(s) 1023
EcoT14I CCWWGG 2 cut(s) 405, 522
EcoT22I ATGCAT 3 cut(s) 90, 547, 1368
ErhI CCWWGG 2 cut(s) 405, 522
FalI AAGNNNNNCTT 2 cut(s) 1001, 1033
FaqI GGGAC 3 cut(s) 374, 382, 820
FauI CCCGC 1 cut(s) 399
FbaI TGATCA 1 cut(s) 735
FblI GTMKAC 2 cut(s) 335, 913
Fnu4HI GCNGC 6 cut(s) 40, 812, 978, 981, 1395, 1431
FokI GGATG 8 cut(s) 494, 539, 619, 857, 935, 1180, 1321, 1337
Fsp4HI GCNGC 6 cut(s) 40, 812, 978, 981, 1395, 1431
FspBI CTAG 4 cut(s) 498, 636, 1280, 1415
FspI TGCGCA 1 cut(s) 1156
GlaI GCGC 3 cut(s) 423, 853, 1156
GluI GCNGC 6 cut(s) 40, 812, 978, 981, 1395, 1431
GsaI CCCAGC 2 cut(s) 162, 1549
HaeII RGCGCY 2 cut(s) 425, 855
HaeIII GGCC 4 cut(s) 177, 255, 521, 1117
HapII CCGG 2 cut(s) 611, 1322
HgaI GACGC 3 cut(s) 281, 593, 817
HhaI GCGC 3 cut(s) 424, 854, 1157
Hin6I GCGC 3 cut(s) 422, 852, 1155
HinP1I GCGC 3 cut(s) 422, 852, 1155
HincII GTYRAC 2 cut(s) 673, 936
HindII GTYRAC 2 cut(s) 673, 936
HinfI GANTC 8 cut(s) 73, 397, 754, 1456, 1553, 1571, 1583, 1633
HpaI GTTAAC 1 cut(s) 673
HpaII CCGG 2 cut(s) 611, 1322
HphI GGTGA 4 cut(s) 51, 980, 1286, 1397
Hpy166II GTNNAC 7 cut(s) 336, 673, 825, 889, 914, 936, 1255
Hpy188I TCNGA 7 cut(s) 106, 170, 659, 759, 1217, 1582, 1661
Hpy188III TCNNGA 2 cut(s) 122, 790
Hpy8I GTNNAC 7 cut(s) 336, 673, 825, 889, 914, 936, 1255
Hpy99I CGWCG 1 cut(s) 294
HpyAV CCTTC 1 cut(s) 1172
HpyCH4III ACNGT 4 cut(s) 373, 436, 730, 1106
HpyCH4IV ACGT 2 cut(s) 165, 292
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 1073, 1148, 1318
HpyF3I CTNAG 1 cut(s) 1652
HpySE526I ACGT 2 cut(s) 165, 292
HspAI GCGC 3 cut(s) 422, 852, 1155
Ksp22I TGATCA 1 cut(s) 735
KspAI GTTAAC 1 cut(s) 673
Kzo9I GATC 3 cut(s) 106, 735, 998
LmnI GCTCC 2 cut(s) 689, 1234
Lsp1109I GCAGC 5 cut(s) 798, 964, 992, 1381, 1417
LweI GCATC 6 cut(s) 597, 760, 835, 906, 1111, 1299
MaeI CTAG 4 cut(s) 498, 636, 1280, 1415
MaeII ACGT 2 cut(s) 165, 292
MaeIII GTNAC 7 cut(s) 557, 581, 925, 1126, 1344, 1421, 1572
MalI GATC 3 cut(s) 108, 737, 1000
MboI GATC 3 cut(s) 106, 735, 998
MboII GAAGA 5 cut(s) 321, 763, 855, 865, 1630
MflI RGATCY 1 cut(s) 998
MhlI GDGCHC 2 cut(s) 891, 1491
MlsI TGGCCA 1 cut(s) 177
MluCI AATT 5 cut(s) 93, 566, 761, 1030, 1035
MluNI TGGCCA 1 cut(s) 177
MlyI GAGTC 3 cut(s) 1562, 1580, 1592
Mox20I TGGCCA 1 cut(s) 177
Mph1103I ATGCAT 3 cut(s) 90, 547, 1368
MscI TGGCCA 1 cut(s) 177
MseI TTAA 7 cut(s) 285, 378, 672, 902, 1173, 1194, 1496
MslI CAYNNNNRTG 2 cut(s) 83, 87
Msp20I TGGCCA 1 cut(s) 177
MspI CCGG 2 cut(s) 611, 1322
MspR9I CCNGG 2 cut(s) 235, 1027
Mva1269I GAATGC 4 cut(s) 503, 547, 1366, 1540
MvaI CCWGG 2 cut(s) 235, 1027
MwoI GCNNNNNNNGC 4 cut(s) 61, 1073, 1148, 1318
NdeII GATC 3 cut(s) 106, 735, 998
NlaIV GGNNCC 4 cut(s) 19, 390, 1000, 1224
NmeAIII GCCGAG 1 cut(s) 398
NmuCI GTSAC 3 cut(s) 581, 1421, 1572
NsbI TGCGCA 1 cut(s) 1156
NsiI ATGCAT 3 cut(s) 90, 547, 1368
NspI RCATGY 3 cut(s) 88, 92, 580
PaeI GCATGC 2 cut(s) 92, 580
PaeR7I CTCGAG 1 cut(s) 1490
PaqCI CACCTGC 1 cut(s) 1262
PceI AGGCCT 1 cut(s) 1117
PctI GAATGC 4 cut(s) 503, 547, 1366, 1540
PfeI GAWTC 5 cut(s) 73, 397, 754, 1456, 1633
PfoI TCCNGGA 2 cut(s) 233, 1025
PkrI GCNGC 6 cut(s) 41, 813, 979, 982, 1396, 1432
PleI GAGTC 3 cut(s) 1561, 1579, 1591
PpsI GAGTC 3 cut(s) 1561, 1579, 1591
Psp6I CCWGG 2 cut(s) 233, 1025
PspFI CCCAGC 2 cut(s) 158, 1545
PspGI CCWGG 2 cut(s) 233, 1025
PspN4I GGNNCC 4 cut(s) 19, 390, 1000, 1224
PspPI GGNCC 3 cut(s) 253, 388, 1255
PspXI VCTCGAGB 1 cut(s) 1490
PstI CTGCAG 2 cut(s) 982, 1399
PstNI CAGNNNCTG 1 cut(s) 1659
PsuI RGATCY 1 cut(s) 998
RsaI GTAC 2 cut(s) 164, 1646
RsaNI GTAC 2 cut(s) 163, 1645
RseI CAYNNNNRTG 2 cut(s) 83, 87
SaqAI TTAA 7 cut(s) 285, 378, 672, 902, 1173, 1194, 1496
SatI GCNGC 6 cut(s) 40, 812, 978, 981, 1395, 1431
Sau3AI GATC 3 cut(s) 106, 735, 998
Sau96I GGNCC 3 cut(s) 253, 388, 1255
SchI GAGTC 3 cut(s) 1562, 1580, 1592
ScrFI CCNGG 2 cut(s) 235, 1027
SduI GDGCHC 2 cut(s) 891, 1491
SfaNI GCATC 6 cut(s) 597, 760, 835, 906, 1111, 1299
SfcI CTRYAG 2 cut(s) 978, 1395
Sfr274I CTCGAG 1 cut(s) 1490
SinI GGWCC 2 cut(s) 388, 1255
SlaI CTCGAG 1 cut(s) 1490
SmiMI CAYNNNNRTG 2 cut(s) 83, 87
SmlI CTYRAG 1 cut(s) 1490
SmoI CTYRAG 1 cut(s) 1490
SpeI ACTAGT 1 cut(s) 635
SphI GCATGC 2 cut(s) 92, 580
Sse9I AATT 5 cut(s) 93, 566, 761, 1030, 1035
SseBI AGGCCT 1 cut(s) 1117
SsiI CCGC 6 cut(s) 4, 40, 62, 392, 966, 1289
SspMI CTAG 4 cut(s) 498, 636, 1280, 1415
StuI AGGCCT 1 cut(s) 1117
StyD4I CCNGG 2 cut(s) 233, 1025
StyI CCWWGG 2 cut(s) 405, 522
TaaI ACNGT 4 cut(s) 373, 436, 730, 1106
TaiI ACGT 2 cut(s) 168, 295
TaqI TCGA 6 cut(s) 109, 204, 289, 507, 554, 1491
TaqII GACCGA 1 cut(s) 866
TasI AATT 5 cut(s) 93, 566, 761, 1030, 1035
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 5 cut(s) 73, 397, 754, 1456, 1633
Tru1I TTAA 7 cut(s) 285, 378, 672, 902, 1173, 1194, 1496
Tru9I TTAA 7 cut(s) 285, 378, 672, 902, 1173, 1194, 1496
TscAI CASTG 1 cut(s) 745
TseFI GTSAC 3 cut(s) 581, 1421, 1572
TseI GCWGC 5 cut(s) 811, 977, 980, 1394, 1430
Tsp45I GTSAC 3 cut(s) 581, 1421, 1572
TspDTI ATGAA 6 cut(s) 222, 389, 473, 1217, 1448, 1523
TspGWI ACGGA 1 cut(s) 676
TspRI CASTG 1 cut(s) 745
VneI GTGCAC 1 cut(s) 887
VpaK11BI GGWCC 2 cut(s) 388, 1255
XapI RAATTY 2 cut(s) 761, 1030
XceI RCATGY 3 cut(s) 88, 92, 580
XhoI CTCGAG 1 cut(s) 1490
XmiI GTMKAC 2 cut(s) 335, 913
XspI CTAG 4 cut(s) 498, 636, 1280, 1415
Zsp2I ATGCAT 3 cut(s) 90, 547, 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.