Rh3BG315000

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0017589
Physical Location & Seq
Forward (+)
32090835 .. 32093037
2203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG315000.1

Sequence Viewer

Length: 1560 bp
ATGCGGAGGTTAGAGGGAAGCATACTCCTAACGACTTGCCGCCAGTTCTGTAAGCTCTCACCGCCCAGTTTGAATGCAATTCCATTTTCTGATCGAAATCCCACTCCCCAAAACAACATTGTGTCATGGAACAAAATGATTGCCGGGTACGTCAGAAATGGCCAAATGCGAGTTGCCCACAACCTGTTCGATGAAATGCCTGTCAAAGACGAAGTTTCCTGGAACACCATCTTATCGGGCCTGCACAACACGGAAGACCCAGATGGAGTTAATCGATGTTTCTTGCAAATGAGAAGAGATGGGTTTAGACCAAATGAGTATACCATCTCCATAGTATTGAGAGCCTTTTTGGGGACTGTGCTTAATGTTTTGGTCCCGCAGATTCATGCCTTGGCAATACTCTCGGCGCTCAACTCAACTGTGTTTGTTGGGTCGGCACTTATGAAAGGGTATGCAAATATAGGGGATGGAGTAGCTATGGCTAGAGTATTCGATGAGATTTCGGCCAAGGATGTGTCATCTTGGAATGCATTGATTTCGAGTTACATGGAATTGGGGTGCATGCATGACGCTCAAAGAGTTTTTGATGGGATGCCGGAGAGGAATATAGTTTCTTGGACTAGTTTAGTAAATGGGTATATCAGAAATAGGAGGGTTAACAAGGCTCGCTCCGTTTTCAATAAAATGAGTGCAAAAAATGTGGTCTCATGGACTGTTATGATCAGTGGTTATGTGAAGAATCAGAAATTTGTAGATGCTTTGGAACTTTTCCTCTTGATGTTGAAGTCAGGGACTCTGCCCAATCAGTTCACATTTTCAAGTGTGCTGGATGCAAGCGCTGGTTGTTCTTCACTTATCTTCGGTCAGCAAGTACACTCACGCATCTTAAAGTCTGGTATACCGGAGGATGTAACCTTGTCAACCTCGCTTGTTGATATGTATGCAAAATGCGGTGATATTGCTGCAGCGTTTTGTATTTTTGGATCCATGCCAAAGAAAAACTTGATATCCTGGAATTCAGTTATAGGAGGTTATGCAAGGCATGGATTTGCTACAAGAGCATTGGAGGAATTTGAGAGAATGACTAACTGTGGTGTTAGGCCTGATGCAGTTACATTCACAAACGCGTTATCCGCTTGTGCACATGGTGGGATGATTGAAGAAGGAAAAAGCCTCTTTAACACCATGAAGTCAAAGTTCGGAATGGAACCACAGGTAGAGCATTATGCTTGCATGGTGGACCTCTATGGGAAAGCAGGTGAGCTAGAGGAAGCGGAGAAGTTGATACATGGGATGCCATTTCAGCCGGATGTTGTTATTTGGTGTGCGTTACTTGGTGCTTGTGGTTTGCATTCAAGTTTGGAACTTGGTGAGTTTGCGGCAGAGGAACTTGATAAACTAGAAAGTGACCATGCTGCAATATATTCAACGCTTTCAAGGATTCATGGGGAAAGAGGAGTTTGGAATAGTGTGCTCGAGTTAAGGAAGACAATGAAAGAGAAAGGCGTTCAAAAGCAGAATGCAGGTAGCTGGGTTGAGTCTGCACGAGTAATTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

519

Amino Acids

57.46

Weight (kDa)

7.93

Isoelectric Point (pI)

45.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 39 - 67 9.7e-08 PPR repeat family
PPR PF01535 41 - 67 2.7e-06 PPR repeat
PPR_2 PF13041 70 - 116 3.6e-10 PPR repeat family
PPR_2 PF13041 201 - 234 1.2e-06 PPR repeat family
PPR PF01535 204 - 233 2.1e-06 PPR repeat
PPR_2 PF13041 232 - 278 2.4e-12 PPR repeat family
PPR PF01535 235 - 264 4e-07 PPR repeat
PPR_2 PF13041 334 - 382 1.6e-08 PPR repeat family
E_motif PF20431 451 - 513 1.7e-10 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017589)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26830 FvH4_1g26830
malus_domestica MD17G1278700.v1.1
prunus_persica Prupe.3G007300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0487071
rosa_laevigata RLG00000023035
rosa_multiflora Rmu_sc0005856.1_g000034
rosa_roxburghii Rroxscaffold_6G00395200
rosa_samantha Rh3BG315000 Rh3CG313400 Rh3DG310000
rosa_wichuraiana Rw3G024740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1247
Acc36I ACCTGC 2 cut(s) 1247, 1514
AccI GTMKAC 2 cut(s) 320, 898
AccII CGCG 1 cut(s) 1127
AciI CCGC 8 cut(s) 4, 40, 62, 377, 951, 1134, 1274, 1379
AclWI GGATC 2 cut(s) 978, 991
AcoI YGGCCR 2 cut(s) 160, 504
AcsI RAATTY 3 cut(s) 746, 1015, 1070
AdeI CACNNNGTG 1 cut(s) 1148
AfaI GTAC 2 cut(s) 149, 873
AfeI AGCGCT 1 cut(s) 838
AfiI CCNNNNNNNGG 1 cut(s) 351
AflIII ACRYGT 1 cut(s) 1125
AgsI TTSAA 9 cut(s) 73, 679, 784, 819, 1160, 1356, 1428, 1437, 1511
AhlI ACTAGT 1 cut(s) 620
AjnI CCWGG 2 cut(s) 218, 1010
AluBI AGCT 4 cut(s) 55, 476, 1264, 1530
AluI AGCT 4 cut(s) 55, 476, 1264, 1530
Alw21I GWGCWC 2 cut(s) 1144, 1476
Alw26I GTCTC 1 cut(s) 709
Alw44I GTGCAC 1 cut(s) 1140
AlwI GGATC 2 cut(s) 978, 991
Ama87I CYCGRG 1 cut(s) 1475
Aor51HI AGCGCT 1 cut(s) 838
AoxI GGCC 4 cut(s) 160, 238, 504, 1100
ApaLI GTGCAC 1 cut(s) 1140
ApeKI GCWGC 3 cut(s) 962, 965, 1415
ApoI RAATTY 3 cut(s) 746, 1015, 1070
AspLEI GCGC 2 cut(s) 409, 839
AspS9I GGNCC 3 cut(s) 238, 373, 1240
AsuC2I CCSGG 1 cut(s) 145
AsuHPI GGTGA 4 cut(s) 51, 965, 1271, 1382
AvaI CYCGRG 1 cut(s) 1475
AvaII GGWCC 2 cut(s) 373, 1240
BaeGI GKGCMC 1 cut(s) 1144
BalI TGGCCA 1 cut(s) 162
BamHI GGATCC 1 cut(s) 983
BauI CACGAG 1 cut(s) 1545
BbsI GAAGAC 2 cut(s) 261, 1493
Bbv12I GWGCWC 2 cut(s) 1144, 1476
BbvI GCAGC 3 cut(s) 949, 977, 1402
BccI CCATC 6 cut(s) 236, 257, 293, 332, 461, 581
BcgI CGANNNNNNTGC 4 cut(s) 384, 418, 519, 553
BciT130I CCWGG 2 cut(s) 220, 1012
BclI TGATCA 1 cut(s) 720
BcnI CCSGG 1 cut(s) 145
BcoDI GTCTC 1 cut(s) 709
BcuI ACTAGT 1 cut(s) 620
BfaI CTAG 4 cut(s) 483, 621, 1265, 1400
BfmI CTRYAG 1 cut(s) 963
BfoI RGCGCY 2 cut(s) 410, 840
BfuAI ACCTGC 2 cut(s) 1247, 1514
BisI GCNGC 5 cut(s) 40, 963, 966, 1380, 1416
BlsI GCNGC 5 cut(s) 41, 964, 967, 1381, 1417
Bme1390I CCNGG 3 cut(s) 145, 220, 1012
Bme18I GGWCC 2 cut(s) 373, 1240
BmeT110I CYCGRG 1 cut(s) 1475
BmgT120I GGNCC 3 cut(s) 238, 373, 1240
BmiI GGNNCC 3 cut(s) 375, 985, 1209
BmrFI CCNGG 3 cut(s) 145, 220, 1012
BmrI ACTGGG 1 cut(s) 60
BmsI GCATC 6 cut(s) 582, 745, 820, 891, 1096, 1284
BmuI ACTGGG 1 cut(s) 60
BpiI GAAGAC 2 cut(s) 261, 1493
BpuMI CCSGG 1 cut(s) 145
Bsa29I ATCGAT 1 cut(s) 274
BsaBI GATNNNNATC 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 709
BsaJI CCNNGG 2 cut(s) 390, 507
BsaWI WCCGGW 1 cut(s) 901
Bsc4I CCNNNNNNNGG 1 cut(s) 351
Bse1I ACTGG 2 cut(s) 43, 66
Bse8I GATNNNNATC 1 cut(s) 96
BseBI CCWGG 2 cut(s) 220, 1012
BseCI ATCGAT 1 cut(s) 274
BseDI CCNNGG 2 cut(s) 390, 507
BseGI GGATG 8 cut(s) 472, 517, 597, 835, 913, 1158, 1299, 1315
BseJI GATNNNNATC 1 cut(s) 96
BseLI CCNNNNNNNGG 1 cut(s) 351
BseNI ACTGG 2 cut(s) 43, 66
BseRI GAGGAG 1 cut(s) 1470
BseSI GKGCMC 1 cut(s) 1144
BseXI GCAGC 3 cut(s) 949, 977, 1402
BseYI CCCAGC 1 cut(s) 1530
BsgI GTGCAG 2 cut(s) 227, 1527
Bsh1236I CGCG 1 cut(s) 1127
BshFI GGCC 4 cut(s) 162, 240, 506, 1102
BshVI ATCGAT 1 cut(s) 274
BsiHKAI GWGCWC 2 cut(s) 1144, 1476
BsiHKCI CYCGRG 1 cut(s) 1475
BsiSI CCGG 4 cut(s) 144, 596, 902, 1307
BslFI GGGAC 3 cut(s) 359, 367, 805
BslI CCNNNNNNNGG 1 cut(s) 351
BsmAI GTCTC 1 cut(s) 709
BsmFI GGGAC 3 cut(s) 359, 367, 805
BsmI GAATGC 4 cut(s) 79, 532, 1351, 1525
BsnI GGCC 4 cut(s) 162, 240, 506, 1102
Bso31I GGTCTC 1 cut(s) 709
BsoBI CYCGRG 1 cut(s) 1475
Bsp1286I GDGCHC 2 cut(s) 1144, 1476
Bsp143I GATC 3 cut(s) 91, 720, 983
BspACI CCGC 8 cut(s) 4, 40, 62, 377, 951, 1134, 1274, 1379
BspANI GGCC 4 cut(s) 162, 240, 506, 1102
BspDI ATCGAT 1 cut(s) 274
BspFNI CGCG 1 cut(s) 1127
BspLI GGNNCC 3 cut(s) 375, 985, 1209
BspMAI CTGCAG 1 cut(s) 967
BspMI ACCTGC 2 cut(s) 1247, 1514
BspPI GGATC 2 cut(s) 978, 991
BspTNI GGTCTC 1 cut(s) 709
BsrI ACTGG 2 cut(s) 43, 66
BssECI CCNNGG 2 cut(s) 390, 507
BssMI GATC 3 cut(s) 91, 720, 983
BssNAI GTATAC 2 cut(s) 321, 899
BssSI CACGAG 1 cut(s) 1545
BssT1I CCWWGG 2 cut(s) 390, 507
Bst1107I GTATAC 2 cut(s) 321, 899
Bst2BI CACGAG 1 cut(s) 1545
Bst2UI CCWGG 2 cut(s) 220, 1012
Bst4CI ACNGT 4 cut(s) 358, 421, 715, 1091
Bst6I CTCTTC 1 cut(s) 289
BstC8I GCNNGC 5 cut(s) 242, 563, 667, 835, 1231
BstF5I GGATG 8 cut(s) 472, 517, 597, 835, 913, 1158, 1299, 1315
BstFNI CGCG 1 cut(s) 1127
BstH2I RGCGCY 2 cut(s) 410, 840
BstHHI GCGC 2 cut(s) 409, 839
BstKTI GATC 3 cut(s) 94, 723, 986
BstMAI GTCTC 1 cut(s) 709
BstMBI GATC 3 cut(s) 91, 720, 983
BstMWI GCNNNNNNNGC 4 cut(s) 61, 1058, 1133, 1303
BstNI CCWGG 2 cut(s) 220, 1012
BstNSI RCATGY 1 cut(s) 565
BstSCI CCNGG 3 cut(s) 143, 218, 1010
BstSFI CTRYAG 1 cut(s) 963
BstSLI GKGCMC 1 cut(s) 1144
BstUI CGCG 1 cut(s) 1127
BstV1I GCAGC 3 cut(s) 949, 977, 1402
BstV2I GAAGAC 2 cut(s) 261, 1493
BstX2I RGATCY 1 cut(s) 983
BstYI RGATCY 1 cut(s) 983
BstZ17I GTATAC 2 cut(s) 321, 899
Bsu15I ATCGAT 1 cut(s) 274
BsuRI GGCC 4 cut(s) 162, 240, 506, 1102
BsuTUI ATCGAT 1 cut(s) 274
BtsCI GGATG 8 cut(s) 472, 517, 597, 835, 913, 1158, 1299, 1315
BtsIMutI CAGTG 1 cut(s) 730
BveI ACCTGC 2 cut(s) 1247, 1514
Cac8I GCNNGC 5 cut(s) 242, 563, 667, 835, 1231
CfoI GCGC 2 cut(s) 409, 839
Cfr13I GGNCC 3 cut(s) 238, 373, 1240
ClaI ATCGAT 1 cut(s) 274
CseI GACGC 1 cut(s) 578
Csp6I GTAC 2 cut(s) 148, 872
CspCI CAANNNNNGTGG 2 cut(s) 681, 716
CviQI GTAC 2 cut(s) 148, 872
DpnI GATC 3 cut(s) 93, 722, 985
DpnII GATC 3 cut(s) 91, 720, 983
DraIII CACNNNGTG 1 cut(s) 1148
EaeI YGGCCR 2 cut(s) 160, 504
Eam1104I CTCTTC 1 cut(s) 289
EarI CTCTTC 1 cut(s) 289
Eco130I CCWWGG 2 cut(s) 390, 507
Eco147I AGGCCT 1 cut(s) 1102
Eco31I GGTCTC 1 cut(s) 709
Eco32I GATATC 1 cut(s) 1008
Eco47I GGWCC 2 cut(s) 373, 1240
Eco47III AGCGCT 1 cut(s) 838
Eco88I CYCGRG 1 cut(s) 1475
EcoRI GAATTC 1 cut(s) 1015
EcoRII CCWGG 2 cut(s) 218, 1010
EcoRV GATATC 1 cut(s) 1008
EcoT14I CCWWGG 2 cut(s) 390, 507
EcoT22I ATGCAT 2 cut(s) 532, 567
ErhI CCWWGG 2 cut(s) 390, 507
FalI AAGNNNNNCTT 2 cut(s) 986, 1018
FaqI GGGAC 3 cut(s) 359, 367, 805
FauI CCCGC 1 cut(s) 384
FbaI TGATCA 1 cut(s) 720
FblI GTMKAC 2 cut(s) 320, 898
Fnu4HI GCNGC 5 cut(s) 40, 963, 966, 1380, 1416
FokI GGATG 8 cut(s) 479, 524, 604, 842, 920, 1165, 1306, 1322
Fsp4HI GCNGC 5 cut(s) 40, 963, 966, 1380, 1416
FspBI CTAG 4 cut(s) 483, 621, 1265, 1400
GlaI GCGC 2 cut(s) 408, 838
GluI GCNGC 5 cut(s) 40, 963, 966, 1380, 1416
GsaI CCCAGC 1 cut(s) 1534
HaeII RGCGCY 2 cut(s) 410, 840
HaeIII GGCC 4 cut(s) 162, 240, 506, 1102
HapII CCGG 4 cut(s) 144, 596, 902, 1307
HgaI GACGC 1 cut(s) 578
HhaI GCGC 2 cut(s) 409, 839
Hin6I GCGC 2 cut(s) 407, 837
HinP1I GCGC 2 cut(s) 407, 837
HincII GTYRAC 2 cut(s) 658, 921
HindII GTYRAC 2 cut(s) 658, 921
HinfI GANTC 5 cut(s) 382, 739, 793, 1441, 1538
HpaI GTTAAC 1 cut(s) 658
HpaII CCGG 4 cut(s) 144, 596, 902, 1307
HphI GGTGA 4 cut(s) 51, 965, 1271, 1382
Hpy166II GTNNAC 8 cut(s) 321, 658, 810, 874, 899, 921, 1142, 1240
Hpy188I TCNGA 5 cut(s) 91, 155, 644, 744, 1202
Hpy188III TCNNGA 1 cut(s) 775
Hpy8I GTNNAC 8 cut(s) 321, 658, 810, 874, 899, 921, 1142, 1240
HpyAV CCTTC 1 cut(s) 1157
HpyCH4III ACNGT 4 cut(s) 358, 421, 715, 1091
HpyCH4IV ACGT 1 cut(s) 150
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 1058, 1133, 1303
HpySE526I ACGT 1 cut(s) 150
HspAI GCGC 2 cut(s) 407, 837
Ksp22I TGATCA 1 cut(s) 720
KspAI GTTAAC 1 cut(s) 658
Kzo9I GATC 3 cut(s) 91, 720, 983
LmnI GCTCC 1 cut(s) 674
Lsp1109I GCAGC 3 cut(s) 949, 977, 1402
LweI GCATC 6 cut(s) 582, 745, 820, 891, 1096, 1284
MaeI CTAG 4 cut(s) 483, 621, 1265, 1400
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 5 cut(s) 542, 910, 1111, 1329, 1406
MalI GATC 3 cut(s) 93, 722, 985
MboI GATC 3 cut(s) 91, 720, 983
MboII GAAGA 7 cut(s) 266, 306, 748, 840, 850, 1172, 1498
MflI RGATCY 1 cut(s) 983
MhlI GDGCHC 2 cut(s) 1144, 1476
MlsI TGGCCA 1 cut(s) 162
MluCI AATT 6 cut(s) 78, 551, 746, 1015, 1070, 1551
MluI ACGCGT 1 cut(s) 1125
MluNI TGGCCA 1 cut(s) 162
MlyI GAGTC 2 cut(s) 787, 1547
Mox20I TGGCCA 1 cut(s) 162
Mph1103I ATGCAT 2 cut(s) 532, 567
MscI TGGCCA 1 cut(s) 162
MseI TTAA 6 cut(s) 270, 363, 657, 887, 1179, 1481
Msp20I TGGCCA 1 cut(s) 162
MspI CCGG 4 cut(s) 144, 596, 902, 1307
MspR9I CCNGG 3 cut(s) 145, 220, 1012
Mva1269I GAATGC 4 cut(s) 79, 532, 1351, 1525
MvaI CCWGG 2 cut(s) 220, 1012
MvnI CGCG 1 cut(s) 1127
MwoI GCNNNNNNNGC 4 cut(s) 61, 1058, 1133, 1303
NciI CCSGG 1 cut(s) 145
NdeII GATC 3 cut(s) 91, 720, 983
NlaIV GGNNCC 3 cut(s) 375, 985, 1209
NmeAIII GCCGAG 1 cut(s) 383
NmuCI GTSAC 1 cut(s) 1406
NsiI ATGCAT 2 cut(s) 532, 567
NspI RCATGY 1 cut(s) 565
PaeI GCATGC 1 cut(s) 565
PaeR7I CTCGAG 1 cut(s) 1475
PaqCI CACCTGC 1 cut(s) 1247
PceI AGGCCT 1 cut(s) 1102
PctI GAATGC 4 cut(s) 79, 532, 1351, 1525
PfeI GAWTC 3 cut(s) 382, 739, 1441
PfoI TCCNGGA 2 cut(s) 218, 1010
PkrI GCNGC 5 cut(s) 41, 964, 967, 1381, 1417
PleI GAGTC 2 cut(s) 787, 1546
PpsI GAGTC 2 cut(s) 787, 1546
Psp6I CCWGG 2 cut(s) 218, 1010
PspFI CCCAGC 1 cut(s) 1530
PspGI CCWGG 2 cut(s) 218, 1010
PspN4I GGNNCC 3 cut(s) 375, 985, 1209
PspPI GGNCC 3 cut(s) 238, 373, 1240
PspXI VCTCGAGB 1 cut(s) 1475
PstI CTGCAG 1 cut(s) 967
PsuI RGATCY 1 cut(s) 983
RsaI GTAC 2 cut(s) 149, 873
RsaNI GTAC 2 cut(s) 148, 872
SaqAI TTAA 6 cut(s) 270, 363, 657, 887, 1179, 1481
SatI GCNGC 5 cut(s) 40, 963, 966, 1380, 1416
Sau3AI GATC 3 cut(s) 91, 720, 983
Sau96I GGNCC 3 cut(s) 238, 373, 1240
SchI GAGTC 2 cut(s) 787, 1547
ScrFI CCNGG 3 cut(s) 145, 220, 1012
SduI GDGCHC 2 cut(s) 1144, 1476
SfaNI GCATC 6 cut(s) 582, 745, 820, 891, 1096, 1284
SfcI CTRYAG 1 cut(s) 963
Sfr274I CTCGAG 1 cut(s) 1475
SinI GGWCC 2 cut(s) 373, 1240
SlaI CTCGAG 1 cut(s) 1475
SmlI CTYRAG 1 cut(s) 1475
SmoI CTYRAG 1 cut(s) 1475
SpeI ACTAGT 1 cut(s) 620
SphI GCATGC 1 cut(s) 565
Sse9I AATT 6 cut(s) 78, 551, 746, 1015, 1070, 1551
SseBI AGGCCT 1 cut(s) 1102
SsiI CCGC 8 cut(s) 4, 40, 62, 377, 951, 1134, 1274, 1379
SspMI CTAG 4 cut(s) 483, 621, 1265, 1400
StuI AGGCCT 1 cut(s) 1102
StyD4I CCNGG 3 cut(s) 143, 218, 1010
StyI CCWWGG 2 cut(s) 390, 507
TaaI ACNGT 4 cut(s) 358, 421, 715, 1091
TaiI ACGT 1 cut(s) 153
TaqI TCGA 6 cut(s) 94, 189, 274, 492, 539, 1476
TaqII GACCGA 1 cut(s) 851
TasI AATT 6 cut(s) 78, 551, 746, 1015, 1070, 1551
TatI WGTACW 1 cut(s) 871
TauI GCSGC 2 cut(s) 42, 1382
TfiI GAWTC 3 cut(s) 382, 739, 1441
Tru1I TTAA 6 cut(s) 270, 363, 657, 887, 1179, 1481
Tru9I TTAA 6 cut(s) 270, 363, 657, 887, 1179, 1481
TscAI CASTG 1 cut(s) 730
TseFI GTSAC 1 cut(s) 1406
TseI GCWGC 3 cut(s) 962, 965, 1415
Tsp45I GTSAC 1 cut(s) 1406
TspDTI ATGAA 6 cut(s) 207, 374, 458, 1202, 1433, 1508
TspGWI ACGGA 2 cut(s) 266, 661
TspRI CASTG 1 cut(s) 730
VneI GTGCAC 1 cut(s) 1140
VpaK11BI GGWCC 2 cut(s) 373, 1240
XapI RAATTY 3 cut(s) 746, 1015, 1070
XceI RCATGY 1 cut(s) 565
XhoI CTCGAG 1 cut(s) 1475
XmiI GTMKAC 2 cut(s) 320, 898
XspI CTAG 4 cut(s) 483, 621, 1265, 1400
Zsp2I ATGCAT 2 cut(s) 532, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.