Rroxscaffold_6G00395200

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0017589
Physical Location & Seq
Reverse (-)
16198450 .. 16200024
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00395200.1

Sequence Viewer

Length: 1575 bp
ATGCGGAGGTTAGAGGGAACCATACTGCTAACGACTGGGCGCCAGTTCTGTAAGCTCTCACCGCCCAGTTTGAATCACATCCACATGCATGCAATTCCATTTTCTGATCGAAATCCCATTCCACAAAACAACATTGTGTCATGGAACAAAATGATTGCCGAGTACGTCCGAAATGGCCAAATGCGAGTTGCCCGCAACCTGTTCGATGAAATGCCTCTGAAAGACGAAGTTTCCTGGAACACCATCTTATCGGGCCTGCACAACACCAAAGACCCAGATGGAGTTAATCGATGTTTCTTGCAAATGAGAAGAGATGGGTTTAGGCCAAATGAGTATACCATCTCCATAGTATTGAGAGCCTTTTTGGGGACTGTGCTTAATGTTTTGGTCCCGCAGATTCATGCCTTGGCAATACTCTCGGCGCTCAACTCAACTGTGTTTGTTGGGTCGGCACTTATGAAAGGGTATGCAAATATAGGGGATGGAGTAGCTACGGCTAGAGTATTCGATGAGATTTTGGCCAAGGATGTGTCATCTTGGAATGCATTGATTTCGAGTTACATGGAATTGGGTTGCATGCATGACGCTCAAAGAGCTTTTGATGGGATGCCGGAGAGGAATATAGTTTCTTGGACTAGTTTAGTAAATGGGTATATCAGAAATAGGAGGGTTAACAAAGCTCGCTCCGTTTTCAATAAAATGAGTGCAAAAAATGTGGTTTCATGGACTGTTATGATCAGTGGTTATGTGAAGAATCAGAAATTTGTAGATGCTTTGGAACTTTTCCTCTTGATGTTGAAGTCAGGGACTCTGCCCAATCAGTTCACATTTTCAAGTGTGCTGGATGCAAGCGCTGGTTGTTCTTCACTTATCTTTGGTCAGCAAGTACACTTACGCATCTTAAAGTCTGGTATACCGGAGGATGTAACCTTGTCAACCTCGCTTGTTGATATGTATGCAAAATGCGGTGATATTGCTGCAGCGTTTTGTATTTTTGGATCCATGCCAAAGAAAAACTTGATATCCTGGAATTCAATTATAGGAGGTTATGCAAGGCATGGATTTGCTACAAGAGCATTGGAGGAGTTTGAGAAAATGACCAACTGTGGTGTTAGGCCTGATGCAGTTACATTCACAAATACATTATCAGCTTGTGCACATGGTGGGATGATTGAAGAAGGAAAAAGCCTCTTTAACACCATGAAGTCAAAGTTCGGAATGGAACCACAGGTGGAGCATTATGCTTGCATGGTGGACCTCTATGGGAAAGCAGGTGAGCTAGAGGAAGCGGAGAAGTTGATACATGGGATGCCATTTCAGCCGGATGTTGTTATTTGGTGTACGTTACTTGGTGCTTGTGGTTTGCATTCAAGTTTGGAATTTGGTGAGTTTGCTGCAGAGGAACTTGATAAACTAGAAAGTGACCATGCTGCAATATATTCAACGCTTTCAAGGATTCATGGGGAAAGAGGAGTTTGGAATAGTGTGCTCGAGTTAAGGAAGACAATGAAAGAGAAAGGCGTTCAAAAGCAGAATGCAGGTAGCTGGGTTGAGTCTGCACGAGTAATTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

524

Amino Acids

58.27

Weight (kDa)

8.39

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 44 - 72 1.8e-06 PPR repeat family
PPR_2 PF13041 75 - 121 1.8e-10 PPR repeat family
PPR_2 PF13041 206 - 239 1.2e-06 PPR repeat family
PPR PF01535 209 - 238 2.1e-06 PPR repeat
PPR_2 PF13041 237 - 283 2.5e-12 PPR repeat family
PPR PF01535 240 - 269 4e-07 PPR repeat
PPR_2 PF13041 339 - 387 3e-09 PPR repeat family
E_motif PF20431 456 - 518 5e-10 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017589)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26830 FvH4_1g26830
malus_domestica MD17G1278700.v1.1
prunus_persica Prupe.3G007300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0487071
rosa_laevigata RLG00000023035
rosa_multiflora Rmu_sc0005856.1_g000034
rosa_roxburghii Rroxscaffold_6G00395200
rosa_samantha Rh3BG315000 Rh3CG313400 Rh3DG310000
rosa_wichuraiana Rw3G024740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1262
Acc36I ACCTGC 2 cut(s) 1262, 1529
AccB1I GGYRCC 1 cut(s) 39
AccI GTMKAC 2 cut(s) 335, 913
AciI CCGC 6 cut(s) 4, 62, 193, 392, 966, 1289
AclWI GGATC 2 cut(s) 993, 1006
AcoI YGGCCR 2 cut(s) 175, 519
AcsI RAATTY 3 cut(s) 761, 1030, 1379
AcyI GRCGYC 1 cut(s) 40
AdeI CACNNNGTG 1 cut(s) 1163
AfaI GTAC 3 cut(s) 164, 888, 1342
AfeI AGCGCT 1 cut(s) 853
AfiI CCNNNNNNNGG 1 cut(s) 366
AhlI ACTAGT 1 cut(s) 635
AjnI CCWGG 2 cut(s) 233, 1025
AluBI AGCT 7 cut(s) 55, 491, 596, 680, 1151, 1279, 1545
AluI AGCT 7 cut(s) 55, 491, 596, 680, 1151, 1279, 1545
Alw21I GWGCWC 2 cut(s) 1159, 1491
Alw44I GTGCAC 1 cut(s) 1155
AlwI GGATC 2 cut(s) 993, 1006
Ama87I CYCGRG 1 cut(s) 1490
Aor51HI AGCGCT 1 cut(s) 853
AoxI GGCC 5 cut(s) 175, 253, 323, 519, 1115
ApaLI GTGCAC 1 cut(s) 1155
ApeKI GCWGC 4 cut(s) 977, 980, 1394, 1430
ApoI RAATTY 3 cut(s) 761, 1030, 1379
AspLEI GCGC 3 cut(s) 42, 424, 854
AspS9I GGNCC 3 cut(s) 253, 388, 1255
AsuHPI GGTGA 4 cut(s) 51, 980, 1286, 1397
AvaI CYCGRG 1 cut(s) 1490
AvaII GGWCC 2 cut(s) 388, 1255
BaeGI GKGCMC 1 cut(s) 1159
BalI TGGCCA 2 cut(s) 177, 521
BamHI GGATCC 1 cut(s) 998
BanI GGYRCC 1 cut(s) 39
BauI CACGAG 1 cut(s) 1560
BbsI GAAGAC 1 cut(s) 1508
Bbv12I GWGCWC 2 cut(s) 1159, 1491
BbvI GCAGC 4 cut(s) 964, 992, 1381, 1417
BccI CCATC 6 cut(s) 251, 272, 308, 347, 476, 596
BceAI ACGGC 1 cut(s) 510
BcgI CGANNNNNNTGC 6 cut(s) 184, 218, 399, 433, 534, 568
BciT130I CCWGG 2 cut(s) 235, 1027
BclI TGATCA 1 cut(s) 735
BcuI ACTAGT 1 cut(s) 635
BfaI CTAG 4 cut(s) 498, 636, 1280, 1415
BfmI CTRYAG 2 cut(s) 978, 1395
BfoI RGCGCY 3 cut(s) 43, 425, 855
BfuAI ACCTGC 2 cut(s) 1262, 1529
BisI GCNGC 4 cut(s) 978, 981, 1395, 1431
BlsI GCNGC 4 cut(s) 979, 982, 1396, 1432
Bme1390I CCNGG 2 cut(s) 235, 1027
Bme18I GGWCC 2 cut(s) 388, 1255
BmeT110I CYCGRG 1 cut(s) 1490
BmgT120I GGNCC 3 cut(s) 253, 388, 1255
BmiI GGNNCC 5 cut(s) 19, 41, 390, 1000, 1224
BmrFI CCNGG 2 cut(s) 235, 1027
BmrI ACTGGG 2 cut(s) 45, 60
BmsI GCATC 6 cut(s) 597, 760, 835, 906, 1111, 1299
BmuI ACTGGG 2 cut(s) 45, 60
BpiI GAAGAC 1 cut(s) 1508
Bsa29I ATCGAT 1 cut(s) 289
BsaBI GATNNNNATC 1 cut(s) 111
BsaHI GRCGYC 1 cut(s) 40
BsaJI CCNNGG 2 cut(s) 405, 522
BsaWI WCCGGW 1 cut(s) 916
Bsc4I CCNNNNNNNGG 1 cut(s) 366
Bse1I ACTGG 3 cut(s) 40, 43, 66
Bse8I GATNNNNATC 1 cut(s) 111
BseBI CCWGG 2 cut(s) 235, 1027
BseCI ATCGAT 1 cut(s) 289
BseDI CCNNGG 2 cut(s) 405, 522
BseGI GGATG 9 cut(s) 78, 487, 532, 612, 850, 928, 1173, 1314, 1330
BseJI GATNNNNATC 1 cut(s) 111
BseLI CCNNNNNNNGG 1 cut(s) 366
BseNI ACTGG 3 cut(s) 40, 43, 66
BseRI GAGGAG 2 cut(s) 1097, 1485
BseSI GKGCMC 1 cut(s) 1159
BseXI GCAGC 4 cut(s) 964, 992, 1381, 1417
BseYI CCCAGC 1 cut(s) 1545
BsgI GTGCAG 2 cut(s) 242, 1542
BshFI GGCC 5 cut(s) 177, 255, 325, 521, 1117
BshNI GGYRCC 1 cut(s) 39
BshVI ATCGAT 1 cut(s) 289
BsiHKAI GWGCWC 2 cut(s) 1159, 1491
BsiHKCI CYCGRG 1 cut(s) 1490
BsiSI CCGG 3 cut(s) 611, 917, 1322
BslFI GGGAC 3 cut(s) 374, 382, 820
BslI CCNNNNNNNGG 1 cut(s) 366
BsmFI GGGAC 3 cut(s) 374, 382, 820
BsmI GAATGC 3 cut(s) 547, 1366, 1540
BsnI GGCC 5 cut(s) 177, 255, 325, 521, 1117
BsoBI CYCGRG 1 cut(s) 1490
Bsp1286I GDGCHC 2 cut(s) 1159, 1491
Bsp143I GATC 3 cut(s) 106, 735, 998
BspACI CCGC 6 cut(s) 4, 62, 193, 392, 966, 1289
BspANI GGCC 5 cut(s) 177, 255, 325, 521, 1117
BspDI ATCGAT 1 cut(s) 289
BspLI GGNNCC 5 cut(s) 19, 41, 390, 1000, 1224
BspMAI CTGCAG 2 cut(s) 982, 1399
BspMI ACCTGC 2 cut(s) 1262, 1529
BspPI GGATC 2 cut(s) 993, 1006
BspT107I GGYRCC 1 cut(s) 39
BsrI ACTGG 3 cut(s) 40, 43, 66
BssECI CCNNGG 2 cut(s) 405, 522
BssMI GATC 3 cut(s) 106, 735, 998
BssNAI GTATAC 2 cut(s) 336, 914
BssNI GRCGYC 1 cut(s) 40
BssSI CACGAG 1 cut(s) 1560
BssT1I CCWWGG 2 cut(s) 405, 522
Bst1107I GTATAC 2 cut(s) 336, 914
Bst2BI CACGAG 1 cut(s) 1560
Bst2UI CCWGG 2 cut(s) 235, 1027
Bst4CI ACNGT 4 cut(s) 373, 436, 730, 1106
Bst6I CTCTTC 1 cut(s) 304
BstACI GRCGYC 1 cut(s) 40
BstC8I GCNNGC 7 cut(s) 90, 193, 257, 578, 682, 850, 1246
BstF5I GGATG 9 cut(s) 78, 487, 532, 612, 850, 928, 1173, 1314, 1330
BstH2I RGCGCY 3 cut(s) 43, 425, 855
BstHHI GCGC 3 cut(s) 42, 424, 854
BstKTI GATC 3 cut(s) 109, 738, 1001
BstMBI GATC 3 cut(s) 106, 735, 998
BstMWI GCNNNNNNNGC 4 cut(s) 61, 593, 1073, 1318
BstNI CCWGG 2 cut(s) 235, 1027
BstNSI RCATGY 3 cut(s) 88, 92, 580
BstSCI CCNGG 2 cut(s) 233, 1025
BstSFI CTRYAG 2 cut(s) 978, 1395
BstSLI GKGCMC 1 cut(s) 1159
BstV1I GCAGC 4 cut(s) 964, 992, 1381, 1417
BstV2I GAAGAC 1 cut(s) 1508
BstX2I RGATCY 1 cut(s) 998
BstYI RGATCY 1 cut(s) 998
BstZ17I GTATAC 2 cut(s) 336, 914
Bsu15I ATCGAT 1 cut(s) 289
BsuRI GGCC 5 cut(s) 177, 255, 325, 521, 1117
BsuTUI ATCGAT 1 cut(s) 289
BtsCI GGATG 9 cut(s) 78, 487, 532, 612, 850, 928, 1173, 1314, 1330
BtsIMutI CAGTG 1 cut(s) 745
BveI ACCTGC 2 cut(s) 1262, 1529
Cac8I GCNNGC 7 cut(s) 90, 193, 257, 578, 682, 850, 1246
CfoI GCGC 3 cut(s) 42, 424, 854
Cfr13I GGNCC 3 cut(s) 253, 388, 1255
ClaI ATCGAT 1 cut(s) 289
CseI GACGC 1 cut(s) 593
Csp6I GTAC 3 cut(s) 163, 887, 1341
CspCI CAANNNNNGTGG 2 cut(s) 696, 731
CviQI GTAC 3 cut(s) 163, 887, 1341
DinI GGCGCC 1 cut(s) 41
DpnI GATC 3 cut(s) 108, 737, 1000
DpnII GATC 3 cut(s) 106, 735, 998
DraIII CACNNNGTG 1 cut(s) 1163
EaeI YGGCCR 2 cut(s) 175, 519
Eam1104I CTCTTC 1 cut(s) 304
EarI CTCTTC 1 cut(s) 304
Eco130I CCWWGG 2 cut(s) 405, 522
Eco147I AGGCCT 1 cut(s) 1117
Eco32I GATATC 1 cut(s) 1023
Eco47I GGWCC 2 cut(s) 388, 1255
Eco47III AGCGCT 1 cut(s) 853
Eco88I CYCGRG 1 cut(s) 1490
EcoRI GAATTC 1 cut(s) 1030
EcoRII CCWGG 2 cut(s) 233, 1025
EcoRV GATATC 1 cut(s) 1023
EcoT14I CCWWGG 2 cut(s) 405, 522
EcoT22I ATGCAT 3 cut(s) 90, 547, 582
EgeI GGCGCC 1 cut(s) 41
EheI GGCGCC 1 cut(s) 41
ErhI CCWWGG 2 cut(s) 405, 522
FalI AAGNNNNNCTT 2 cut(s) 1001, 1033
FaqI GGGAC 3 cut(s) 374, 382, 820
FauI CCCGC 2 cut(s) 200, 399
FbaI TGATCA 1 cut(s) 735
FblI GTMKAC 2 cut(s) 335, 913
Fnu4HI GCNGC 4 cut(s) 978, 981, 1395, 1431
FokI GGATG 9 cut(s) 65, 494, 539, 619, 857, 935, 1180, 1321, 1337
Fsp4HI GCNGC 4 cut(s) 978, 981, 1395, 1431
FspBI CTAG 4 cut(s) 498, 636, 1280, 1415
GlaI GCGC 3 cut(s) 41, 423, 853
GluI GCNGC 4 cut(s) 978, 981, 1395, 1431
GsaI CCCAGC 1 cut(s) 1549
HaeII RGCGCY 3 cut(s) 43, 425, 855
HaeIII GGCC 5 cut(s) 177, 255, 325, 521, 1117
HapII CCGG 3 cut(s) 611, 917, 1322
HgaI GACGC 1 cut(s) 593
HhaI GCGC 3 cut(s) 42, 424, 854
Hin1I GRCGYC 1 cut(s) 40
Hin6I GCGC 3 cut(s) 40, 422, 852
HinP1I GCGC 3 cut(s) 40, 422, 852
HincII GTYRAC 2 cut(s) 673, 936
HindII GTYRAC 2 cut(s) 673, 936
HinfI GANTC 6 cut(s) 73, 397, 754, 808, 1456, 1553
HpaI GTTAAC 1 cut(s) 673
HpaII CCGG 3 cut(s) 611, 917, 1322
HphI GGTGA 4 cut(s) 51, 980, 1286, 1397
Hpy166II GTNNAC 9 cut(s) 336, 673, 825, 889, 914, 936, 1157, 1255, 1341
Hpy188I TCNGA 6 cut(s) 106, 170, 219, 659, 759, 1217
Hpy188III TCNNGA 1 cut(s) 790
Hpy8I GTNNAC 9 cut(s) 336, 673, 825, 889, 914, 936, 1157, 1255, 1341
HpyAV CCTTC 1 cut(s) 1172
HpyCH4III ACNGT 4 cut(s) 373, 436, 730, 1106
HpyCH4IV ACGT 2 cut(s) 165, 1343
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 593, 1073, 1318
HpySE526I ACGT 2 cut(s) 165, 1343
Hsp92I GRCGYC 1 cut(s) 40
HspAI GCGC 3 cut(s) 40, 422, 852
KasI GGCGCC 1 cut(s) 39
Ksp22I TGATCA 1 cut(s) 735
KspAI GTTAAC 1 cut(s) 673
Kzo9I GATC 3 cut(s) 106, 735, 998
LmnI GCTCC 2 cut(s) 689, 1234
Lsp1109I GCAGC 4 cut(s) 964, 992, 1381, 1417
LweI GCATC 6 cut(s) 597, 760, 835, 906, 1111, 1299
MaeI CTAG 4 cut(s) 498, 636, 1280, 1415
MaeII ACGT 2 cut(s) 165, 1343
MaeIII GTNAC 5 cut(s) 557, 925, 1126, 1344, 1421
MalI GATC 3 cut(s) 108, 737, 1000
MboI GATC 3 cut(s) 106, 735, 998
MboII GAAGA 5 cut(s) 321, 763, 855, 1187, 1513
MflI RGATCY 1 cut(s) 998
MhlI GDGCHC 2 cut(s) 1159, 1491
MlsI TGGCCA 2 cut(s) 177, 521
MluCI AATT 7 cut(s) 93, 566, 761, 1030, 1035, 1379, 1566
MluNI TGGCCA 2 cut(s) 177, 521
Mly113I GGCGCC 1 cut(s) 40
MlyI GAGTC 2 cut(s) 802, 1562
Mox20I TGGCCA 2 cut(s) 177, 521
Mph1103I ATGCAT 3 cut(s) 90, 547, 582
MscI TGGCCA 2 cut(s) 177, 521
MseI TTAA 6 cut(s) 285, 378, 672, 902, 1194, 1496
MslI CAYNNNNRTG 2 cut(s) 83, 87
Msp20I TGGCCA 2 cut(s) 177, 521
MspI CCGG 3 cut(s) 611, 917, 1322
MspR9I CCNGG 2 cut(s) 235, 1027
Mva1269I GAATGC 3 cut(s) 547, 1366, 1540
MvaI CCWGG 2 cut(s) 235, 1027
MwoI GCNNNNNNNGC 4 cut(s) 61, 593, 1073, 1318
NarI GGCGCC 1 cut(s) 40
NdeII GATC 3 cut(s) 106, 735, 998
NlaIV GGNNCC 5 cut(s) 19, 41, 390, 1000, 1224
NmeAIII GCCGAG 2 cut(s) 184, 398
NmuCI GTSAC 1 cut(s) 1421
NsiI ATGCAT 3 cut(s) 90, 547, 582
NspI RCATGY 3 cut(s) 88, 92, 580
PaeI GCATGC 2 cut(s) 92, 580
PaeR7I CTCGAG 1 cut(s) 1490
PaqCI CACCTGC 1 cut(s) 1262
PceI AGGCCT 1 cut(s) 1117
PctI GAATGC 3 cut(s) 547, 1366, 1540
PfeI GAWTC 4 cut(s) 73, 397, 754, 1456
PfoI TCCNGGA 2 cut(s) 233, 1025
PkrI GCNGC 4 cut(s) 979, 982, 1396, 1432
PleI GAGTC 2 cut(s) 802, 1561
PluTI GGCGCC 1 cut(s) 43
PpsI GAGTC 2 cut(s) 802, 1561
Psp6I CCWGG 2 cut(s) 233, 1025
PspFI CCCAGC 1 cut(s) 1545
PspGI CCWGG 2 cut(s) 233, 1025
PspN4I GGNNCC 5 cut(s) 19, 41, 390, 1000, 1224
PspPI GGNCC 3 cut(s) 253, 388, 1255
PspXI VCTCGAGB 1 cut(s) 1490
PstI CTGCAG 2 cut(s) 982, 1399
PsuI RGATCY 1 cut(s) 998
RsaI GTAC 3 cut(s) 164, 888, 1342
RsaNI GTAC 3 cut(s) 163, 887, 1341
RseI CAYNNNNRTG 2 cut(s) 83, 87
SaqAI TTAA 6 cut(s) 285, 378, 672, 902, 1194, 1496
SatI GCNGC 4 cut(s) 978, 981, 1395, 1431
Sau3AI GATC 3 cut(s) 106, 735, 998
Sau96I GGNCC 3 cut(s) 253, 388, 1255
SchI GAGTC 2 cut(s) 802, 1562
ScrFI CCNGG 2 cut(s) 235, 1027
SduI GDGCHC 2 cut(s) 1159, 1491
SfaNI GCATC 6 cut(s) 597, 760, 835, 906, 1111, 1299
SfcI CTRYAG 2 cut(s) 978, 1395
SfoI GGCGCC 1 cut(s) 41
Sfr274I CTCGAG 1 cut(s) 1490
SinI GGWCC 2 cut(s) 388, 1255
SlaI CTCGAG 1 cut(s) 1490
SmiMI CAYNNNNRTG 2 cut(s) 83, 87
SmlI CTYRAG 1 cut(s) 1490
SmoI CTYRAG 1 cut(s) 1490
SpeI ACTAGT 1 cut(s) 635
SphI GCATGC 2 cut(s) 92, 580
Sse9I AATT 7 cut(s) 93, 566, 761, 1030, 1035, 1379, 1566
SseBI AGGCCT 1 cut(s) 1117
SsiI CCGC 6 cut(s) 4, 62, 193, 392, 966, 1289
SspDI GGCGCC 1 cut(s) 39
SspMI CTAG 4 cut(s) 498, 636, 1280, 1415
StuI AGGCCT 1 cut(s) 1117
StyD4I CCNGG 2 cut(s) 233, 1025
StyI CCWWGG 2 cut(s) 405, 522
TaaI ACNGT 4 cut(s) 373, 436, 730, 1106
TaiI ACGT 2 cut(s) 168, 1346
TaqI TCGA 6 cut(s) 109, 204, 289, 507, 554, 1491
TasI AATT 7 cut(s) 93, 566, 761, 1030, 1035, 1379, 1566
TatI WGTACW 1 cut(s) 886
TfiI GAWTC 4 cut(s) 73, 397, 754, 1456
Tru1I TTAA 6 cut(s) 285, 378, 672, 902, 1194, 1496
Tru9I TTAA 6 cut(s) 285, 378, 672, 902, 1194, 1496
TscAI CASTG 1 cut(s) 745
TseFI GTSAC 1 cut(s) 1421
TseI GCWGC 4 cut(s) 977, 980, 1394, 1430
Tsp45I GTSAC 1 cut(s) 1421
TspDTI ATGAA 7 cut(s) 222, 389, 473, 711, 1217, 1448, 1523
TspGWI ACGGA 1 cut(s) 676
TspRI CASTG 1 cut(s) 745
VneI GTGCAC 1 cut(s) 1155
VpaK11BI GGWCC 2 cut(s) 388, 1255
XapI RAATTY 3 cut(s) 761, 1030, 1379
XceI RCATGY 3 cut(s) 88, 92, 580
XhoI CTCGAG 1 cut(s) 1490
XmiI GTMKAC 2 cut(s) 335, 913
XspI CTAG 4 cut(s) 498, 636, 1280, 1415
Zsp2I ATGCAT 3 cut(s) 90, 547, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.