RLG00000024084

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0010236
Physical Location & Seq
Forward (+)
31630600 .. 31633514
2915 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024084

Sequence Viewer

Length: 1566 bp
ATGGCTCAGTTACACGAGCTACTGAAAGAAGATCAGGAGCCGTTTCTTCTCAAGAGCTACATTGCTGACAAACGCGGCCAGCTGAAAAGACCTTCCCCCAAAACTCAATTGCAGGTCAAGAAACGAAGACCCATCTCGGAAGTTTCCAACTTCCCCAGAAACCTATGCAAAAATGCTTGCCTTTTCTCCCTCCAAGACTCGCCGGACCTCCGCAAATCGCCGCTGTTCGAGTTCTCGTCGCCGGCTGCTAAATCCCCATGCAAGAGTCCCAATGCCATTTTTCTCCAAATCCCAACCAGAACCGCAGCTCTTCTCGTCGAAGCTGCCATGAGGATTCAGAAACAGTCGACAAGTTCCAAACCCAAAACCCAGAACCACAGCAAAAACCATGGTTTTGGGCTAATTGGGTCCTTGTTGAAGAGACTAAAAACCCGAACCCAAAAGCGTGAAATCGAAGGGAACGGGGTTAAGGTCTCGGTCAAAGACATTCTCAGGTGGGATTCATCGGTTGGGCGCAGAAAGATTTCAACAGAAGATAGCCAGCTGAAAATGGAGGAGGATAAATTTGATTCTGGTCTGAGTTTGGAGGACCAAAGTGCTTCTGAAATAAGGGCTACTTGTGAGATGTGCTTTGCTTCTTCTAATGTAAACAGTAGGCCCAGTAGCGAAGTTTGGTCTTTGGACATGGACACTTCGAGTAGTTGTCAGTCTGATTACTCGGAAGCGATTTTGGGTACAACAGACTGCGTTTGCTGCGAGACGAAGGTCTTTTGTGAGAGCCCTTTTCGTTTTGTGCTCGAAACAACACCCTCGCAGTCCGGTAGCCGGACGCCGGAGTTCTCATCTCCGGCGACGTCCCCTGTTCGCCACAAACAAGAGGAGAAGGAAGGATTAAAGAATTTTCAAGTTGAAGAAGAGGAGGAAGATAAGGAACAGTGCAGTCCTGTATCAGTACTGGACCCTCCATTCGAAGATGATGACGAGGAGCATGACGAGGAAGACGAAGAACATGGTTTTGATTTGGAATGCAGCTATGCCATTATGCAGAGGACAAAGAAGCAGTTAATGCAGAAGCTTCGTAGATTTGAGAAATTGGCAGAGTTAGATCCTATTGAACTTGAAAAAAGAATGTTAGAAGAAGATGATGATGAAGAAGCAGAATCACAAGAATGTGACGATGATGAGTCGAAAACATCAGAGAGCAGCAGGGAAGAAAATCTTGATGGGTTTGTCAGAGAAGTACTTTGCAAGTCAAGCTTTCCTAGTTCAAAGAGAATTCCGGAAGACGTGAAGACGTTGGTGTCAGATCTCATTGTTGAGGAACACGGAAAAGAGGATGATGGTTTCAGCAATAGAGAAGAGGTGGTGAGAAGGGTCTGCAAGAGGTTTAACTCCTGGAACGAGGTGGAGTCGAACACCATAGATATGATGGTGGAACAAGATTTCAGAAAGGAACTCGATGGGTGGAAGCAAAATCAAGGTCAGGTGGGAGAGACTGCAGTAGACATTGAGCTTGCTATCTTCGGCCTTCTAGTTGAGGAATTGGCAATGGAACTAGTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378

Protein Analysis

522

Amino Acids

59.34

Weight (kDa)

4.8

Isoelectric Point (pI)

73.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 450 - 514 6.2e-06 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1300
AatII GACGTC 1 cut(s) 857
Acc36I ACCTGC 1 cut(s) 103
AccI GTMKAC 2 cut(s) 347, 1503
AccII CGCG 1 cut(s) 75
AccIII TCCGGA 1 cut(s) 1279
AciI CCGC 4 cut(s) 75, 211, 221, 303
AclWI GGATC 1 cut(s) 1100
AcoI YGGCCR 1 cut(s) 76
AcsI RAATTY 3 cut(s) 563, 898, 1275
AcyI GRCGYC 2 cut(s) 830, 854
AfaI GTAC 3 cut(s) 736, 954, 1242
AfiI CCNNNNNNNGG 2 cut(s) 825, 832
AgsI TTSAA 7 cut(s) 418, 528, 905, 911, 1115, 1121, 1269
AhlI ACTAGT 1 cut(s) 1555
AjiI CACGTC 1 cut(s) 1288
AjnI CCWGG 1 cut(s) 1394
Alw21I GWGCWC 1 cut(s) 798
Alw26I GTCTC 4 cut(s) 415, 478, 752, 1487
AlwI GGATC 1 cut(s) 1100
Aor13HI TCCGGA 1 cut(s) 1279
AoxI GGCC 3 cut(s) 76, 656, 1525
ApeKI GCWGC 6 cut(s) 245, 305, 323, 753, 1029, 1203
ApoI RAATTY 3 cut(s) 563, 898, 1275
ArsI GACNNNNNNTTYG 4 cut(s) 950, 982, 1465, 1497
Asp700I GAANNNNTTC 1 cut(s) 523
AspLEI GCGC 1 cut(s) 516
AspS9I GGNCC 5 cut(s) 205, 408, 589, 657, 958
AsuHPI GGTGA 1 cut(s) 1378
AsuII TTCGAA 1 cut(s) 969
AvaII GGWCC 4 cut(s) 205, 408, 589, 958
BanII GRGCYC 1 cut(s) 782
BauI CACGAG 1 cut(s) 14
BbsI GAAGAC 4 cut(s) 133, 1005, 1290, 1298
Bbv12I GWGCWC 1 cut(s) 798
BbvI GCAGC 6 cut(s) 232, 310, 317, 740, 1041, 1215
BccI CCATC 5 cut(s) 140, 1217, 1334, 1423, 1454
BceAI ACGGC 1 cut(s) 25
BcgI CGANNNNNNTGC 2 cut(s) 1058, 1092
BciT130I CCWGG 1 cut(s) 1396
BcoDI GTCTC 4 cut(s) 415, 478, 752, 1487
BcuI ACTAGT 1 cut(s) 1555
BfaI CTAG 3 cut(s) 1263, 1532, 1556
BfmI CTRYAG 1 cut(s) 1497
BfuAI ACCTGC 1 cut(s) 103
BglII AGATCT 1 cut(s) 1306
BisI GCNGC 8 cut(s) 76, 221, 246, 306, 324, 754, 1030, 1204
BlsI GCNGC 8 cut(s) 77, 222, 247, 307, 325, 755, 1031, 1205
BmcAI AGTACT 2 cut(s) 954, 1242
Bme1390I CCNGG 1 cut(s) 1396
Bme18I GGWCC 4 cut(s) 205, 408, 589, 958
BmgBI CACGTC 1 cut(s) 1288
BmgT120I GGNCC 5 cut(s) 205, 408, 589, 657, 958
BmiI GGNNCC 3 cut(s) 39, 409, 960
BmrFI CCNGG 1 cut(s) 1396
BmrI ACTGGG 1 cut(s) 654
BmuI ACTGGG 1 cut(s) 654
BoxI GACNNNNGTC 1 cut(s) 764
BpiI GAAGAC 4 cut(s) 133, 1005, 1290, 1298
Bpu14I TTCGAA 1 cut(s) 969
BpuEI CTTGAG 1 cut(s) 35
BsaHI GRCGYC 2 cut(s) 830, 854
BsaI GGTCTC 1 cut(s) 478
BsaJI CCNNGG 1 cut(s) 388
BsaWI WCCGGW 2 cut(s) 818, 1279
Bsc4I CCNNNNNNNGG 2 cut(s) 825, 832
Bse118I RCCGGY 1 cut(s) 241
Bse1I ACTGG 2 cut(s) 660, 960
Bse3DI GCAATG 2 cut(s) 60, 1554
BseAI TCCGGA 1 cut(s) 1279
BseBI CCWGG 1 cut(s) 1396
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 1 cut(s) 1342
BseLI CCNNNNNNNGG 2 cut(s) 825, 832
BseMI GCAATG 2 cut(s) 60, 1554
BseMII CTCAG 3 cut(s) 20, 505, 569
BseNI ACTGG 2 cut(s) 660, 960
BseRI GAGGAG 4 cut(s) 569, 893, 932, 998
BseXI GCAGC 6 cut(s) 232, 310, 317, 740, 1041, 1215
BsgI GTGCAG 1 cut(s) 958
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 3 cut(s) 78, 658, 1527
BsiHKAI GWGCWC 1 cut(s) 798
BsiSI CCGG 7 cut(s) 203, 242, 819, 826, 833, 848, 1280
BslFI GGGAC 2 cut(s) 252, 841
BslI CCNNNNNNNGG 2 cut(s) 825, 832
BsmAI GTCTC 4 cut(s) 415, 478, 752, 1487
BsmBI CGTCTC 1 cut(s) 752
BsmFI GGGAC 2 cut(s) 252, 841
BsmI GAATGC 1 cut(s) 1031
BsnI GGCC 3 cut(s) 78, 658, 1527
Bso31I GGTCTC 1 cut(s) 478
Bsp119I TTCGAA 1 cut(s) 969
Bsp1286I GDGCHC 2 cut(s) 782, 798
Bsp13I TCCGGA 1 cut(s) 1279
Bsp143I GATC 3 cut(s) 31, 1105, 1306
Bsp19I CCATGG 1 cut(s) 388
BspACI CCGC 4 cut(s) 75, 211, 221, 303
BspANI GGCC 3 cut(s) 78, 658, 1527
BspCNI CTCAG 3 cut(s) 19, 504, 570
BspEI TCCGGA 1 cut(s) 1279
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 3 cut(s) 39, 409, 960
BspMAI CTGCAG 1 cut(s) 1501
BspMI ACCTGC 1 cut(s) 103
BspPI GGATC 1 cut(s) 1100
BspQI GCTCTTC 1 cut(s) 315
BspT104I TTCGAA 1 cut(s) 969
BspTNI GGTCTC 1 cut(s) 478
BsrDI GCAATG 2 cut(s) 60, 1554
BsrFI RCCGGY 1 cut(s) 241
BsrI ACTGG 2 cut(s) 660, 960
BssAI RCCGGY 1 cut(s) 241
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 3 cut(s) 31, 1105, 1306
BssNI GRCGYC 2 cut(s) 830, 854
BssSI CACGAG 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 388
Bst2BI CACGAG 1 cut(s) 14
Bst2UI CCWGG 1 cut(s) 1396
Bst4CI ACNGT 3 cut(s) 345, 653, 936
Bst6I CTCTTC 4 cut(s) 315, 413, 909, 1353
BstACI GRCGYC 2 cut(s) 830, 854
BstAPI GCANNNNNTGC 1 cut(s) 1066
BstBI TTCGAA 1 cut(s) 969
BstC8I GCNNGC 5 cut(s) 80, 178, 243, 542, 1515
BstDEI CTNAG 3 cut(s) 6, 491, 578
BstDSI CCRYGG 1 cut(s) 388
BstF5I GGATG 1 cut(s) 1342
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 1 cut(s) 516
BstKTI GATC 3 cut(s) 34, 1108, 1309
BstMAI GTCTC 4 cut(s) 415, 478, 752, 1487
BstMBI GATC 3 cut(s) 31, 1105, 1306
BstMWI GCNNNNNNNGC 3 cut(s) 753, 1066, 1254
BstNI CCWGG 1 cut(s) 1396
BstPAI GACNNNNGTC 1 cut(s) 764
BstSCI CCNGG 1 cut(s) 1394
BstSFI CTRYAG 1 cut(s) 1497
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 6 cut(s) 232, 310, 317, 740, 1041, 1215
BstV2I GAAGAC 4 cut(s) 133, 1005, 1290, 1298
BstX2I RGATCY 2 cut(s) 1105, 1306
BstXI CCANNNNNNTGG 1 cut(s) 395
BstYI RGATCY 2 cut(s) 1105, 1306
BsuRI GGCC 3 cut(s) 78, 658, 1527
BtgI CCRYGG 1 cut(s) 388
BtrI CACGTC 1 cut(s) 1288
BtsCI GGATG 1 cut(s) 1342
BtsIMutI CAGTG 1 cut(s) 941
BveI ACCTGC 1 cut(s) 103
Cac8I GCNNGC 5 cut(s) 80, 178, 243, 542, 1515
CfoI GCGC 1 cut(s) 516
Cfr10I RCCGGY 1 cut(s) 241
Cfr13I GGNCC 5 cut(s) 205, 408, 589, 657, 958
CseI GACGC 1 cut(s) 838
Csp6I GTAC 3 cut(s) 735, 953, 1241
CviAII CATG 6 cut(s) 258, 328, 389, 685, 989, 1010
CviQI GTAC 3 cut(s) 735, 953, 1241
DdeI CTNAG 3 cut(s) 6, 491, 578
DpnI GATC 3 cut(s) 33, 1107, 1308
DpnII GATC 3 cut(s) 31, 1105, 1306
DrdI GACNNNNNNGTC 1 cut(s) 1300
DseDI GACNNNNNNGTC 1 cut(s) 1300
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 4 cut(s) 315, 413, 909, 1353
EarI CTCTTC 4 cut(s) 315, 413, 909, 1353
Eco130I CCWWGG 1 cut(s) 388
Eco24I GRGCYC 1 cut(s) 782
Eco31I GGTCTC 1 cut(s) 478
Eco47I GGWCC 4 cut(s) 205, 408, 589, 958
EcoO109I RGGNCCY 1 cut(s) 408
EcoRI GAATTC 1 cut(s) 1275
EcoRII CCWGG 1 cut(s) 1394
EcoT14I CCWWGG 1 cut(s) 388
EcoT38I GRGCYC 1 cut(s) 782
ErhI CCWWGG 1 cut(s) 388
Esp3I CGTCTC 1 cut(s) 752
FaeI CATG 6 cut(s) 261, 331, 392, 688, 992, 1013
FalI AAGNNNNNCTT 6 cut(s) 601, 633, 1203, 1235, 1241, 1273
FaqI GGGAC 2 cut(s) 252, 841
FatI CATG 6 cut(s) 257, 327, 388, 684, 988, 1009
FblI GTMKAC 2 cut(s) 347, 1503
Fnu4HI GCNGC 8 cut(s) 76, 221, 246, 306, 324, 754, 1030, 1204
FokI GGATG 1 cut(s) 1349
FriOI GRGCYC 1 cut(s) 782
Fsp4HI GCNGC 8 cut(s) 76, 221, 246, 306, 324, 754, 1030, 1204
FspBI CTAG 3 cut(s) 1263, 1532, 1556
GlaI GCGC 1 cut(s) 515
GluI GCNGC 8 cut(s) 76, 221, 246, 306, 324, 754, 1030, 1204
HaeIII GGCC 3 cut(s) 78, 658, 1527
HapII CCGG 7 cut(s) 203, 242, 819, 826, 833, 848, 1280
HgaI GACGC 1 cut(s) 838
HhaI GCGC 1 cut(s) 516
Hin1I GRCGYC 2 cut(s) 830, 854
Hin1II CATG 6 cut(s) 261, 331, 392, 688, 992, 1013
Hin6I GCGC 1 cut(s) 514
HinP1I GCGC 1 cut(s) 514
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HindIII AAGCTT 2 cut(s) 1073, 1255
HinfI GANTC 8 cut(s) 197, 265, 334, 500, 569, 1160, 1184, 1409
HpaII CCGG 7 cut(s) 203, 242, 819, 826, 833, 848, 1280
HphI GGTGA 1 cut(s) 1378
Hpy166II GTNNAC 3 cut(s) 348, 649, 1504
Hpy188III TCNNGA 5 cut(s) 35, 52, 118, 1220, 1280
Hpy8I GTNNAC 3 cut(s) 348, 649, 1504
Hpy99I CGWCG 3 cut(s) 241, 320, 856
HpyAV CCTTC 7 cut(s) 102, 449, 757, 877, 881, 1365, 1538
HpyCH4III ACNGT 3 cut(s) 345, 653, 936
HpyCH4IV ACGT 3 cut(s) 854, 1287, 1295
HpyF10VI GCNNNNNNNGC 3 cut(s) 753, 1066, 1254
HpyF3I CTNAG 3 cut(s) 6, 491, 578
HpySE526I ACGT 3 cut(s) 854, 1287, 1295
Hsp92I GRCGYC 2 cut(s) 830, 854
Hsp92II CATG 6 cut(s) 261, 331, 392, 688, 992, 1013
HspAI GCGC 1 cut(s) 514
Kpn2I TCCGGA 1 cut(s) 1279
KroI GCCGGC 1 cut(s) 241
KroNI GCCGGC 1 cut(s) 243
Kzo9I GATC 3 cut(s) 31, 1105, 1306
LguI GCTCTTC 1 cut(s) 315
LmnI GCTCC 2 cut(s) 37, 985
Lsp1109I GCAGC 6 cut(s) 232, 310, 317, 740, 1041, 1215
MaeI CTAG 3 cut(s) 1263, 1532, 1556
MaeII ACGT 3 cut(s) 854, 1287, 1295
MaeIII GTNAC 2 cut(s) 9, 1172
MalI GATC 3 cut(s) 33, 1107, 1308
MboI GATC 3 cut(s) 31, 1105, 1306
MfeI CAATTG 1 cut(s) 107
MflI RGATCY 2 cut(s) 1105, 1306
MhlI GDGCHC 2 cut(s) 782, 798
MluCI AATT 7 cut(s) 107, 402, 563, 898, 1091, 1275, 1541
MlyI GAGTC 4 cut(s) 191, 274, 1193, 1418
MmeI TCCRAC 1 cut(s) 171
MroI TCCGGA 1 cut(s) 1279
MroNI GCCGGC 1 cut(s) 241
MroXI GAANNNNTTC 1 cut(s) 523
MseI TTAA 4 cut(s) 468, 893, 1064, 1389
MslI CAYNNNNRTG 2 cut(s) 1168, 1424
MspA1I CMGCKG 3 cut(s) 82, 223, 544
MspI CCGG 7 cut(s) 203, 242, 819, 826, 833, 848, 1280
MspR9I CCNGG 1 cut(s) 1396
MunI CAATTG 1 cut(s) 107
Mva1269I GAATGC 1 cut(s) 1031
MvaI CCWGG 1 cut(s) 1396
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 3 cut(s) 753, 1066, 1254
NaeI GCCGGC 1 cut(s) 243
NcoI CCATGG 1 cut(s) 388
NdeII GATC 3 cut(s) 31, 1105, 1306
NgoMIV GCCGGC 1 cut(s) 241
NlaIII CATG 6 cut(s) 261, 331, 392, 688, 992, 1013
NlaIV GGNNCC 3 cut(s) 39, 409, 960
NmuCI GTSAC 1 cut(s) 1172
NspV TTCGAA 1 cut(s) 969
PciSI GCTCTTC 1 cut(s) 315
PcsI WCGNNNNNNNCGW 1 cut(s) 999
PctI GAATGC 1 cut(s) 1031
PdiI GCCGGC 1 cut(s) 243
PdmI GAANNNNTTC 1 cut(s) 523
PfeI GAWTC 4 cut(s) 334, 500, 569, 1160
PfoI TCCNGGA 1 cut(s) 1394
PkrI GCNGC 8 cut(s) 77, 222, 247, 307, 325, 755, 1031, 1205
PleI GAGTC 4 cut(s) 191, 273, 1192, 1417
PpsI GAGTC 4 cut(s) 191, 273, 1192, 1417
PpuMI RGGWCCY 1 cut(s) 408
PshAI GACNNNNGTC 1 cut(s) 764
Psp5II RGGWCCY 1 cut(s) 408
Psp6I CCWGG 1 cut(s) 1394
PspGI CCWGG 1 cut(s) 1394
PspN4I GGNNCC 3 cut(s) 39, 409, 960
PspPI GGNCC 5 cut(s) 205, 408, 589, 657, 958
PspPPI RGGWCCY 1 cut(s) 408
PstI CTGCAG 1 cut(s) 1501
PsuI RGATCY 2 cut(s) 1105, 1306
PvuII CAGCTG 2 cut(s) 82, 544
RsaI GTAC 3 cut(s) 736, 954, 1242
RsaNI GTAC 3 cut(s) 735, 953, 1241
RseI CAYNNNNRTG 2 cut(s) 1168, 1424
SalI GTCGAC 1 cut(s) 346
SapI GCTCTTC 1 cut(s) 315
SaqAI TTAA 4 cut(s) 468, 893, 1064, 1389
SatI GCNGC 8 cut(s) 76, 221, 246, 306, 324, 754, 1030, 1204
Sau3AI GATC 3 cut(s) 31, 1105, 1306
Sau96I GGNCC 5 cut(s) 205, 408, 589, 657, 958
ScaI AGTACT 2 cut(s) 954, 1242
SchI GAGTC 4 cut(s) 191, 274, 1193, 1418
ScrFI CCNGG 1 cut(s) 1396
SduI GDGCHC 2 cut(s) 782, 798
SfcI CTRYAG 1 cut(s) 1497
SfuI TTCGAA 1 cut(s) 969
SinI GGWCC 4 cut(s) 205, 408, 589, 958
SmiMI CAYNNNNRTG 2 cut(s) 1168, 1424
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
SpeI ACTAGT 1 cut(s) 1555
Sse9I AATT 7 cut(s) 107, 402, 563, 898, 1091, 1275, 1541
SsiI CCGC 4 cut(s) 75, 211, 221, 303
SspMI CTAG 3 cut(s) 1263, 1532, 1556
StyD4I CCNGG 1 cut(s) 1394
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 3 cut(s) 345, 653, 936
TaiI ACGT 3 cut(s) 857, 1290, 1298
TaqII GACCGA 1 cut(s) 466
TasI AATT 7 cut(s) 107, 402, 563, 898, 1091, 1275, 1541
TatI WGTACW 2 cut(s) 952, 1240
TauI GCSGC 2 cut(s) 78, 223
TfiI GAWTC 4 cut(s) 334, 500, 569, 1160
Tru1I TTAA 4 cut(s) 468, 893, 1064, 1389
Tru9I TTAA 4 cut(s) 468, 893, 1064, 1389
TscAI CASTG 1 cut(s) 941
TseFI GTSAC 1 cut(s) 1172
TseI GCWGC 6 cut(s) 245, 305, 323, 753, 1029, 1203
Tsp45I GTSAC 1 cut(s) 1172
TspDTI ATGAA 2 cut(s) 492, 1164
TspGWI ACGGA 1 cut(s) 1341
TspRI CASTG 1 cut(s) 941
VpaK11BI GGWCC 4 cut(s) 205, 408, 589, 958
XapI RAATTY 3 cut(s) 563, 898, 1275
XcmI CCANNNNNNNNNTGG 1 cut(s) 1426
XmiI GTMKAC 2 cut(s) 347, 1503
XmnI GAANNNNTTC 1 cut(s) 523
XspI CTAG 3 cut(s) 1263, 1532, 1556
ZraI GACGTC 1 cut(s) 855
ZrmI AGTACT 2 cut(s) 954, 1242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.