Rroxscaffold_4G00299320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010236
Physical Location & Seq
Forward (+)
19238535 .. 19249055
10521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00299320.1

Sequence Viewer

Length: 513 bp
ATGAAAAAGAAGAAGAAGAACCCGGAAACGCAGCCGGTGAAAAGATCTTCGCCCAAAACCCAATTGCAGGTCAAGAAACAAAGACCCATCTCGGAAGTTTTGAACTTCCAAAGAAATCTATGCAAAACTGCTTGCTTTTTCTCTCTCCAAGTCTCGCCGGACCTCCGGATCCGTTGTTCGAGTTCTCATCGGCGGCGGCTAAATCCCATTATCCGACGGAAGAGAGCTAGAGTGAGAGGTTCGAAGTTACGTAAAGACAACAACTATGCATGGATGATTAACGGAGACAAGCGGCGAGCTCCAAGTAAGTTTCAAGCAATCCACCACGTATACACCTCCGGGAATAGAACCGGACAAGCGGCGAGCTCCAAAATCTCAAGTTCGCAAGACCTATTCATCGAGACACGGATCGGCTCGTATACTAGTAGAAGCTTTGGGCTTCACCCGAAGTCGACCGAAACGGCAATGCTGATGCGAAACGGAGGAAGGAAGCGTCCCCGACGGAAGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378

Protein Analysis

170

Amino Acids

19.75

Weight (kDa)

11.85

Isoelectric Point (pI)

74.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 58
AccI GTMKAC 3 cut(s) 330, 419, 452
AccIII TCCGGA 1 cut(s) 165
AciI CCGC 5 cut(s) 193, 196, 292, 359, 508
AclWI GGATC 3 cut(s) 163, 176, 416
AfiI CCNNNNNNNGG 1 cut(s) 67
AgsI TTSAA 2 cut(s) 103, 314
AhlI ACTAGT 1 cut(s) 422
AloI GAACNNNNNNTCC 2 cut(s) 160, 192
AluBI AGCT 4 cut(s) 227, 299, 366, 432
AluI AGCT 4 cut(s) 227, 299, 366, 432
Alw21I GWGCWC 2 cut(s) 301, 368
Alw26I GTCTC 3 cut(s) 157, 279, 395
AlwI GGATC 3 cut(s) 163, 176, 416
Aor13HI TCCGGA 1 cut(s) 165
ApeKI GCWGC 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 160
AsuC2I CCSGG 2 cut(s) 23, 340
AsuHPI GGTGA 2 cut(s) 49, 434
AsuII TTCGAA 1 cut(s) 242
AvaII GGWCC 1 cut(s) 160
BamHI GGATCC 1 cut(s) 168
BanII GRGCYC 2 cut(s) 301, 368
Bbv12I GWGCWC 2 cut(s) 301, 368
BbvI GCAGC 1 cut(s) 43
BccI CCATC 1 cut(s) 95
BceAI ACGGC 1 cut(s) 477
BcnI CCSGG 2 cut(s) 23, 340
BcoDI GTCTC 3 cut(s) 157, 279, 395
BcuI ACTAGT 1 cut(s) 422
BfaI CTAG 2 cut(s) 228, 423
BfuAI ACCTGC 1 cut(s) 58
BglII AGATCT 1 cut(s) 44
BisI GCNGC 5 cut(s) 32, 194, 197, 293, 360
BlsI GCNGC 5 cut(s) 33, 195, 198, 294, 361
Bme1390I CCNGG 2 cut(s) 23, 340
Bme18I GGWCC 1 cut(s) 160
BmgT120I GGNCC 1 cut(s) 160
BmiI GGNNCC 1 cut(s) 170
BmrFI CCNGG 2 cut(s) 23, 340
BmsI GCATC 1 cut(s) 462
Bpu14I TTCGAA 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 361
BpuMI CCSGG 2 cut(s) 23, 340
BsaAI YACGTR 2 cut(s) 251, 328
BsaWI WCCGGW 2 cut(s) 165, 350
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse118I RCCGGY 1 cut(s) 34
Bse3DI GCAATG 1 cut(s) 471
BseAI TCCGGA 1 cut(s) 165
BseGI GGATG 1 cut(s) 279
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMI GCAATG 1 cut(s) 471
BseXI GCAGC 1 cut(s) 43
Bsh1285I CGRYCG 1 cut(s) 456
BsiEI CGRYCG 1 cut(s) 456
BsiHKAI GWGCWC 2 cut(s) 301, 368
BsiSI CCGG 6 cut(s) 23, 35, 158, 166, 339, 351
BslFI GGGAC 1 cut(s) 480
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 3 cut(s) 157, 279, 395
BsmFI GGGAC 1 cut(s) 480
Bsp119I TTCGAA 1 cut(s) 242
Bsp1286I GDGCHC 2 cut(s) 301, 368
Bsp13I TCCGGA 1 cut(s) 165
Bsp143I GATC 3 cut(s) 44, 168, 408
BspACI CCGC 5 cut(s) 193, 196, 292, 359, 508
BspEI TCCGGA 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 170
BspMI ACCTGC 1 cut(s) 58
BspPI GGATC 3 cut(s) 163, 176, 416
BspT104I TTCGAA 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 471
BsrFI RCCGGY 1 cut(s) 34
BssAI RCCGGY 1 cut(s) 34
BssMI GATC 3 cut(s) 44, 168, 408
BssNAI GTATAC 2 cut(s) 331, 420
Bst1107I GTATAC 2 cut(s) 331, 420
Bst6I CTCTTC 1 cut(s) 215
BstBAI YACGTR 2 cut(s) 251, 328
BstBI TTCGAA 1 cut(s) 242
BstC8I GCNNGC 3 cut(s) 133, 297, 364
BstF5I GGATG 1 cut(s) 279
BstKTI GATC 3 cut(s) 47, 171, 411
BstMAI GTCTC 3 cut(s) 157, 279, 395
BstMBI GATC 3 cut(s) 44, 168, 408
BstMCI CGRYCG 1 cut(s) 456
BstSCI CCNGG 2 cut(s) 21, 338
BstSNI TACGTA 1 cut(s) 251
BstV1I GCAGC 1 cut(s) 43
BstX2I RGATCY 2 cut(s) 44, 168
BstYI RGATCY 2 cut(s) 44, 168
BstZ17I GTATAC 2 cut(s) 331, 420
BtsCI GGATG 1 cut(s) 279
BveI ACCTGC 1 cut(s) 58
Cac8I GCNNGC 3 cut(s) 133, 297, 364
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 160
CseI GACGC 1 cut(s) 482
CviAII CATG 1 cut(s) 270
CviJI RGCY 8 cut(s) 34, 199, 227, 299, 366, 414, 432, 439
CviKI_1 RGCY 8 cut(s) 34, 199, 227, 299, 366, 414, 432, 439
DpnI GATC 3 cut(s) 46, 170, 410
DpnII GATC 3 cut(s) 44, 168, 408
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
Ecl136II GAGCTC 2 cut(s) 299, 366
Eco105I TACGTA 1 cut(s) 251
Eco24I GRGCYC 2 cut(s) 301, 368
Eco47I GGWCC 1 cut(s) 160
Eco53kI GAGCTC 2 cut(s) 299, 366
EcoICRI GAGCTC 2 cut(s) 299, 366
EcoT22I ATGCAT 1 cut(s) 271
EcoT38I GRGCYC 2 cut(s) 301, 368
FaeI CATG 1 cut(s) 273
FaiI YATR 5 cut(s) 121, 267, 271, 331, 420
FaqI GGGAC 1 cut(s) 480
FatI CATG 1 cut(s) 269
FblI GTMKAC 3 cut(s) 330, 419, 452
Fnu4HI GCNGC 5 cut(s) 32, 194, 197, 293, 360
FokI GGATG 1 cut(s) 286
FriOI GRGCYC 2 cut(s) 301, 368
Fsp4HI GCNGC 5 cut(s) 32, 194, 197, 293, 360
FspBI CTAG 2 cut(s) 228, 423
GluI GCNGC 5 cut(s) 32, 194, 197, 293, 360
HapII CCGG 6 cut(s) 23, 35, 158, 166, 339, 351
HgaI GACGC 1 cut(s) 482
Hin1II CATG 1 cut(s) 273
HincII GTYRAC 1 cut(s) 453
HindII GTYRAC 1 cut(s) 453
HindIII AAGCTT 1 cut(s) 430
HpaII CCGG 6 cut(s) 23, 35, 158, 166, 339, 351
HphI GGTGA 2 cut(s) 49, 434
Hpy166II GTNNAC 3 cut(s) 331, 420, 453
Hpy188I TCNGA 2 cut(s) 94, 215
Hpy188III TCNNGA 3 cut(s) 73, 166, 400
Hpy8I GTNNAC 3 cut(s) 331, 420, 453
Hpy99I CGWCG 2 cut(s) 219, 504
HpyAV CCTTC 1 cut(s) 480
HpyCH4IV ACGT 2 cut(s) 250, 327
HpyCH4V TGCA 3 cut(s) 67, 123, 269
HpySE526I ACGT 2 cut(s) 250, 327
Hsp92II CATG 1 cut(s) 273
Kpn2I TCCGGA 1 cut(s) 165
Kzo9I GATC 3 cut(s) 44, 168, 408
LmnI GCTCC 2 cut(s) 304, 371
LpnPI CCDG 7 cut(s) 36, 48, 53, 171, 179, 352, 364
Lsp1109I GCAGC 1 cut(s) 43
LweI GCATC 1 cut(s) 462
MaeI CTAG 2 cut(s) 228, 423
MaeII ACGT 2 cut(s) 250, 327
MaeIII GTNAC 1 cut(s) 246
MalI GATC 3 cut(s) 46, 170, 410
MboI GATC 3 cut(s) 44, 168, 408
MboII GAAGA 5 cut(s) 22, 25, 28, 39, 232
MfeI CAATTG 1 cut(s) 62
MflI RGATCY 2 cut(s) 44, 168
MhlI GDGCHC 2 cut(s) 301, 368
MluCI AATT 1 cut(s) 62
MmeI TCCRAC 1 cut(s) 238
MnlI CCTC 4 cut(s) 173, 230, 346, 476
Mph1103I ATGCAT 1 cut(s) 271
MroI TCCGGA 1 cut(s) 165
MseI TTAA 1 cut(s) 279
MspI CCGG 6 cut(s) 23, 35, 158, 166, 339, 351
MspR9I CCNGG 2 cut(s) 23, 340
MunI CAATTG 1 cut(s) 62
NciI CCSGG 2 cut(s) 23, 340
NdeII GATC 3 cut(s) 44, 168, 408
NlaIII CATG 1 cut(s) 273
NlaIV GGNNCC 1 cut(s) 170
NsiI ATGCAT 1 cut(s) 271
NspV TTCGAA 1 cut(s) 242
PfoI TCCNGGA 1 cut(s) 338
PkrI GCNGC 5 cut(s) 33, 195, 198, 294, 361
Ppu21I YACGTR 2 cut(s) 251, 328
Psp124BI GAGCTC 2 cut(s) 301, 368
PspN4I GGNNCC 1 cut(s) 170
PspPI GGNCC 1 cut(s) 160
PsuI RGATCY 2 cut(s) 44, 168
SacI GAGCTC 2 cut(s) 301, 368
SalI GTCGAC 1 cut(s) 451
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 5 cut(s) 32, 194, 197, 293, 360
Sau3AI GATC 3 cut(s) 44, 168, 408
Sau96I GGNCC 1 cut(s) 160
ScrFI CCNGG 2 cut(s) 23, 340
SduI GDGCHC 2 cut(s) 301, 368
SfaNI GCATC 1 cut(s) 462
SfuI TTCGAA 1 cut(s) 242
SinI GGWCC 1 cut(s) 160
SmlI CTYRAG 1 cut(s) 376
SmoI CTYRAG 1 cut(s) 376
SnaBI TACGTA 1 cut(s) 251
SpeI ACTAGT 1 cut(s) 422
Sse9I AATT 1 cut(s) 62
SsiI CCGC 5 cut(s) 193, 196, 292, 359, 508
SspMI CTAG 2 cut(s) 228, 423
SstI GAGCTC 2 cut(s) 301, 368
StyD4I CCNGG 2 cut(s) 21, 338
TaiI ACGT 2 cut(s) 253, 330
TaqI TCGA 4 cut(s) 179, 242, 399, 452
TaqII GACCGA 1 cut(s) 470
TasI AATT 1 cut(s) 62
TauI GCSGC 4 cut(s) 196, 199, 295, 362
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TseI GCWGC 1 cut(s) 31
TspDTI ATGAA 2 cut(s) 17, 385
TspGWI ACGGA 5 cut(s) 161, 232, 297, 421, 495
VpaK11BI GGWCC 1 cut(s) 160
XmiI GTMKAC 3 cut(s) 330, 419, 452
XspI CTAG 2 cut(s) 228, 423
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.