RLG00000025035
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017795
Physical Location & Seq
Forward (+)
41468267 .. 41470113
1847 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025035

Sequence Viewer

Length: 987 bp
ATGGAATTAGGGGCGGGAGGGGTAGTGAATACCGTGTGGGAGTTGTACGAGAGTAAGGGGAAGATAGGGAGTGGACGCTACGGAGCTGTGCATGGGGCGGTTCAAAAGGCTACGGGCCAAACTGTGGCCTTGAAGGAATTCGAGCACCCAGGTGATGGGCAAGGCTTCACTCCTGAGATTCTTCGAGAAGTCGCTCTTCTCAAAGACCTGAATCAGTCCAGATATGTTGTAGATCTCATCGACGTTGAGACTCCAAGATCATCGGATACAAGTATCTATCTCGTTTTCGAGTATCTGGACATGAACTTGGGCCACTACATTAGCAAGTTTGAACCAGGAATATTAATGCCGAGACGGACCATCAAGATGTTTGTGTTTAGTCTCTGCAAGGGGATGGCTCACATTCACTCACATTTTGTGAGACATCGAGACCTCAAACCTGACAATCTTTTCGTGGATGAAAAGCGAATGATCCTCAAGATCGGAGATCTTGGAGTTAGTCGCTCCATTACTACCCCCGCCACGGTTTTAACACCTGACATGGTCAGCTACCACTATAGGGCTCCTGAAATTTACTTGGGAGGCAGATACGGCCGCCCGGTCGACATGTGGTCTGTAGGATGTGTATTCGCTGAGATGTTGCTGGGCAAACCTCTATTCTATGGACGTGTTAAGGAACAAGCATTGAGCTCCATTTTCAGGACTATAGGAGTACCAACGGAGGAGCAGTGGCCTGATTGCACTACTCTGCCAAATTGGAATCCCTATTGGAATGCACAAGCTTCTGGAGGAGCTGTGGGCTTGGAAGGGATAATTCCTGACATTGATGCATATGGACTTGGCCTTTTATATGTAGGTGTTCTCACTATTATAGAATGGTTACCTATGATCCTGCAAAATGAATTCCTACGGAGAAGCTATGAAATGTCCTCATTGACCAAGAAACTTAAGTTTGAAGACTTGACAGTTTTGAAGAATGGGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000307 GO:0000902 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0009058 GO:0009059 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010033 GO:0010103 GO:0010154 GO:0010374 GO:0010376 GO:0010389 GO:0010440 GO:0010444 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022414 GO:0023052 GO:0030154 GO:0030332 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0032991 GO:0034641 GO:0034645 GO:0036211 GO:0040007 GO:0042023 GO:0042127 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044770 GO:0044772 GO:0044786 GO:0044843 GO:0046483 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051239 GO:0051716 GO:0051726 GO:0060255 GO:0060560 GO:0061458 GO:0061695 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097472 GO:0099402 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901990 GO:1902494 GO:1902554 GO:1902749 GO:1902806 GO:1902911 GO:1903047 GO:1990234 GO:2000026 GO:2000037 GO:2000112
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

329

Amino Acids

36.87

Weight (kDa)

6.46

Isoelectric Point (pI)

36.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 16 - 250 2.5e-51 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 19 - 243 5e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017795)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0460201 RchiOBHm_Chr3g0460211
rosa_laevigata RLG00000025035
rosa_multiflora Rmu_sc0000449.1_g000043 Rmu_sc0003763.1_g000004
rosa_roxburghii Rroxscaffold_6G00419740
rosa_rugosa Rorug03G0041100
rosa_samantha Rh3AG098600 Rh3BG101800 Rh3CG103900 Rh3DG103100
rosa_wichuraiana Rw3G008340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 124, 155
AccI GTMKAC 1 cut(s) 603
AciI CCGC 4 cut(s) 14, 98, 519, 595
AclWI GGATC 2 cut(s) 466, 883
AcoI YGGCCR 1 cut(s) 592
AcsI RAATTY 3 cut(s) 137, 570, 902
AfaI GTAC 2 cut(s) 47, 714
AfiI CCNNNNNNNGG 5 cut(s) 124, 155, 523, 559, 699
AflII CTTAAG 1 cut(s) 947
AflIII ACRYGT 2 cut(s) 606, 667
AgsI TTSAA 5 cut(s) 104, 133, 332, 956, 973
AhdI GACNNNNNGTC 1 cut(s) 610
AjiI CACGTC 1 cut(s) 668
AjnI CCWGG 2 cut(s) 148, 334
AleI CACNNNNGTG 1 cut(s) 150
AluBI AGCT 6 cut(s) 86, 549, 690, 782, 794, 918
AluI AGCT 6 cut(s) 86, 549, 690, 782, 794, 918
Alw21I GWGCWC 2 cut(s) 147, 692
Alw26I GTCTC 5 cut(s) 242, 346, 386, 415, 423
AlwI GGATC 2 cut(s) 466, 883
AoxI GGCC 6 cut(s) 115, 126, 310, 592, 731, 841
ApoI RAATTY 3 cut(s) 137, 570, 902
ArsI GACNNNNNNTTYG 2 cut(s) 434, 466
AseI ATTAAT 1 cut(s) 344
Asp700I GAANNNNTTC 1 cut(s) 137
AspS9I GGNCC 3 cut(s) 115, 310, 357
AsuC2I CCSGG 1 cut(s) 599
AsuHPI GGTGA 1 cut(s) 164
AvaII GGWCC 1 cut(s) 357
BanII GRGCYC 2 cut(s) 565, 692
BbsI GAAGAC 1 cut(s) 963
Bbv12I GWGCWC 2 cut(s) 147, 692
BccI CCATC 3 cut(s) 149, 368, 388
BceAI ACGGC 1 cut(s) 607
BciT130I CCWGG 2 cut(s) 150, 336
BciVI GTATCC 1 cut(s) 259
BcnI CCSGG 1 cut(s) 599
BcoDI GTCTC 5 cut(s) 242, 346, 386, 415, 423
BfmI CTRYAG 3 cut(s) 556, 615, 705
BfrI CTTAAG 1 cut(s) 947
BfuI GTATCC 1 cut(s) 259
BglII AGATCT 2 cut(s) 232, 487
BisI GCNGC 1 cut(s) 595
BlsI GCNGC 1 cut(s) 596
Bme1390I CCNGG 3 cut(s) 150, 336, 599
Bme18I GGWCC 1 cut(s) 357
BmeRI GACNNNNNGTC 1 cut(s) 610
BmgBI CACGTC 1 cut(s) 668
BmgT120I GGNCC 3 cut(s) 115, 310, 357
BmiI GGNNCC 1 cut(s) 564
BmrFI CCNGG 3 cut(s) 150, 336, 599
BmsI GCATC 1 cut(s) 817
BpiI GAAGAC 1 cut(s) 963
BpmI CTGGAG 1 cut(s) 807
BpuEI CTTGAG 1 cut(s) 461
BpuMI CCSGG 1 cut(s) 599
BsaI GGTCTC 1 cut(s) 423
BsaJI CCNNGG 2 cut(s) 148, 522
BsaXI ACNNNNNCTCC 6 cut(s) 9, 39, 713, 743, 780, 810
Bsc4I CCNNNNNNNGG 5 cut(s) 124, 155, 523, 559, 699
BseBI CCWGG 2 cut(s) 150, 336
BseDI CCNNGG 2 cut(s) 148, 522
BseGI GGATG 3 cut(s) 399, 463, 626
BseLI CCNNNNNNNGG 5 cut(s) 124, 155, 523, 559, 699
BseMII CTCAG 2 cut(s) 165, 624
BseRI GAGGAG 2 cut(s) 737, 804
BseX3I CGGCCG 1 cut(s) 592
BseYI CCCAGC 1 cut(s) 643
Bsh1285I CGRYCG 2 cut(s) 595, 603
BshFI GGCC 6 cut(s) 117, 128, 312, 594, 733, 843
BsiEI CGRYCG 2 cut(s) 595, 603
BsiHKAI GWGCWC 2 cut(s) 147, 692
BsiSI CCGG 1 cut(s) 599
BslI CCNNNNNNNGG 5 cut(s) 124, 155, 523, 559, 699
BsmAI GTCTC 5 cut(s) 242, 346, 386, 415, 423
BsmBI CGTCTC 1 cut(s) 346
BsmI GAATGC 1 cut(s) 778
BsnI GGCC 6 cut(s) 117, 128, 312, 594, 733, 843
Bso31I GGTCTC 1 cut(s) 423
Bsp1286I GDGCHC 3 cut(s) 147, 565, 692
Bsp143I GATC 6 cut(s) 232, 257, 471, 480, 487, 888
BspACI CCGC 4 cut(s) 14, 98, 519, 595
BspANI GGCC 6 cut(s) 117, 128, 312, 594, 733, 843
BspCNI CTCAG 2 cut(s) 166, 625
BspLI GGNNCC 1 cut(s) 564
BspPI GGATC 2 cut(s) 466, 883
BspQI GCTCTTC 1 cut(s) 201
BspTI CTTAAG 1 cut(s) 947
BspTNI GGTCTC 1 cut(s) 423
BssECI CCNNGG 2 cut(s) 148, 522
BssMI GATC 6 cut(s) 232, 257, 471, 480, 487, 888
Bst2UI CCWGG 2 cut(s) 150, 336
Bst4CI ACNGT 4 cut(s) 34, 124, 526, 967
Bst6I CTCTTC 1 cut(s) 201
BstAFI CTTAAG 1 cut(s) 947
BstDEI CTNAG 2 cut(s) 174, 633
BstDSI CCRYGG 1 cut(s) 522
BstEII GGTNACC 1 cut(s) 879
BstF5I GGATG 3 cut(s) 399, 463, 626
BstKTI GATC 6 cut(s) 235, 260, 474, 483, 490, 891
BstMAI GTCTC 5 cut(s) 242, 346, 386, 415, 423
BstMBI GATC 6 cut(s) 232, 257, 471, 480, 487, 888
BstMCI CGRYCG 2 cut(s) 595, 603
BstMWI GCNNNNNNNGC 1 cut(s) 591
BstNI CCWGG 2 cut(s) 150, 336
BstNSI RCATGY 1 cut(s) 610
BstPI GGTNACC 1 cut(s) 879
BstSCI CCNGG 3 cut(s) 148, 334, 597
BstSFI CTRYAG 3 cut(s) 556, 615, 705
BstV2I GAAGAC 1 cut(s) 963
BstX2I RGATCY 2 cut(s) 232, 487
BstYI RGATCY 2 cut(s) 232, 487
BstZI CGGCCG 1 cut(s) 592
BsuI GTATCC 1 cut(s) 259
BsuRI GGCC 6 cut(s) 117, 128, 312, 594, 733, 843
BtgI CCRYGG 1 cut(s) 522
BtrI CACGTC 1 cut(s) 668
BtsCI GGATG 3 cut(s) 399, 463, 626
BtsI GCAGTG 1 cut(s) 734
BtsIMutI CAGTG 1 cut(s) 734
Cfr13I GGNCC 3 cut(s) 115, 310, 357
CseI GACGC 1 cut(s) 84
Csp6I GTAC 2 cut(s) 46, 713
CviAII CATG 4 cut(s) 92, 301, 541, 607
CviQI GTAC 2 cut(s) 46, 713
DdeI CTNAG 2 cut(s) 174, 633
DpnI GATC 6 cut(s) 234, 259, 473, 482, 489, 890
DpnII GATC 6 cut(s) 232, 257, 471, 480, 487, 888
DriI GACNNNNNGTC 1 cut(s) 610
EaeI YGGCCR 1 cut(s) 592
EagI CGGCCG 1 cut(s) 592
Eam1104I CTCTTC 1 cut(s) 201
Eam1105I GACNNNNNGTC 1 cut(s) 610
EarI CTCTTC 1 cut(s) 201
Ecl136II GAGCTC 1 cut(s) 690
EclXI CGGCCG 1 cut(s) 592
Eco24I GRGCYC 2 cut(s) 565, 692
Eco31I GGTCTC 1 cut(s) 423
Eco47I GGWCC 1 cut(s) 357
Eco52I CGGCCG 1 cut(s) 592
Eco53kI GAGCTC 1 cut(s) 690
Eco91I GGTNACC 1 cut(s) 879
EcoICRI GAGCTC 1 cut(s) 690
EcoO65I GGTNACC 1 cut(s) 879
EcoRI GAATTC 2 cut(s) 137, 902
EcoRII CCWGG 2 cut(s) 148, 334
EcoT22I ATGCAT 1 cut(s) 832
EcoT38I GRGCYC 2 cut(s) 565, 692
Esp3I CGTCTC 1 cut(s) 346
FaeI CATG 4 cut(s) 95, 304, 544, 610
FalI AAGNNNNNCTT 2 cut(s) 180, 212
FatI CATG 4 cut(s) 91, 300, 540, 606
FauI CCCGC 2 cut(s) 7, 526
FauNDI CATATG 1 cut(s) 832
FblI GTMKAC 1 cut(s) 603
Fnu4HI GCNGC 1 cut(s) 595
FokI GGATG 3 cut(s) 406, 470, 633
FriOI GRGCYC 2 cut(s) 565, 692
Fsp4HI GCNGC 1 cut(s) 595
GluI GCNGC 1 cut(s) 595
GsaI CCCAGC 1 cut(s) 647
GsuI CTGGAG 1 cut(s) 807
HaeIII GGCC 6 cut(s) 117, 128, 312, 594, 733, 843
HapII CCGG 1 cut(s) 599
HgaI GACGC 1 cut(s) 84
Hin1II CATG 4 cut(s) 95, 304, 544, 610
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HindIII AAGCTT 1 cut(s) 780
HinfI GANTC 4 cut(s) 178, 211, 250, 760
HpaII CCGG 1 cut(s) 599
HphI GGTGA 1 cut(s) 164
Hpy166II GTNNAC 2 cut(s) 74, 604
Hpy188I TCNGA 2 cut(s) 265, 485
Hpy8I GTNNAC 2 cut(s) 74, 604
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 2 cut(s) 127, 800
HpyCH4III ACNGT 4 cut(s) 34, 124, 526, 967
HpyCH4IV ACGT 2 cut(s) 243, 667
HpyCH4V TGCA 6 cut(s) 91, 387, 741, 776, 830, 895
HpyF10VI GCNNNNNNNGC 1 cut(s) 591
HpyF3I CTNAG 2 cut(s) 174, 633
HpySE526I ACGT 2 cut(s) 243, 667
Hsp92II CATG 4 cut(s) 95, 304, 544, 610
Kzo9I GATC 6 cut(s) 232, 257, 471, 480, 487, 888
LguI GCTCTTC 1 cut(s) 201
LmnI GCTCC 6 cut(s) 83, 509, 568, 695, 724, 791
LweI GCATC 1 cut(s) 817
MaeII ACGT 2 cut(s) 243, 667
MaeIII GTNAC 1 cut(s) 879
MalI GATC 6 cut(s) 234, 259, 473, 482, 489, 890
MboI GATC 6 cut(s) 232, 257, 471, 480, 487, 888
MboII GAAGA 5 cut(s) 73, 173, 188, 968, 985
MflI RGATCY 2 cut(s) 232, 487
MhlI GDGCHC 3 cut(s) 147, 565, 692
MluCI AATT 6 cut(s) 5, 137, 570, 754, 813, 902
MlyI GAGTC 1 cut(s) 244
MnlI CCTC 8 cut(s) 11, 443, 485, 575, 663, 715, 782, 940
Mph1103I ATGCAT 1 cut(s) 832
MroXI GAANNNNTTC 1 cut(s) 137
MseI TTAA 4 cut(s) 344, 530, 672, 948
MslI CAYNNNNRTG 2 cut(s) 150, 365
MspCI CTTAAG 1 cut(s) 947
MspI CCGG 1 cut(s) 599
MspR9I CCNGG 3 cut(s) 150, 336, 599
Mva1269I GAATGC 1 cut(s) 778
MvaI CCWGG 2 cut(s) 150, 336
MwoI GCNNNNNNNGC 1 cut(s) 591
NciI CCSGG 1 cut(s) 599
NdeI CATATG 1 cut(s) 832
NdeII GATC 6 cut(s) 232, 257, 471, 480, 487, 888
NlaIII CATG 4 cut(s) 95, 304, 544, 610
NlaIV GGNNCC 1 cut(s) 564
NmeAIII GCCGAG 1 cut(s) 375
NsiI ATGCAT 1 cut(s) 832
NspI RCATGY 1 cut(s) 610
OliI CACNNNNGTG 1 cut(s) 150
PciI ACATGT 1 cut(s) 606
PciSI GCTCTTC 1 cut(s) 201
PctI GAATGC 1 cut(s) 778
PdmI GAANNNNTTC 1 cut(s) 137
PfeI GAWTC 3 cut(s) 178, 211, 760
PflFI GACNNNGTC 1 cut(s) 542
PflMI CCANNNNNTGG 2 cut(s) 124, 155
PkrI GCNGC 1 cut(s) 596
PleI GAGTC 1 cut(s) 244
PpsI GAGTC 1 cut(s) 244
PscI ACATGT 1 cut(s) 606
PshBI ATTAAT 1 cut(s) 344
Psp124BI GAGCTC 1 cut(s) 692
Psp6I CCWGG 2 cut(s) 148, 334
PspEI GGTNACC 1 cut(s) 879
PspFI CCCAGC 1 cut(s) 643
PspGI CCWGG 2 cut(s) 148, 334
PspN4I GGNNCC 1 cut(s) 564
PspPI GGNCC 3 cut(s) 115, 310, 357
PsuI RGATCY 2 cut(s) 232, 487
PsyI GACNNNGTC 1 cut(s) 542
RsaI GTAC 2 cut(s) 47, 714
RsaNI GTAC 2 cut(s) 46, 713
RseI CAYNNNNRTG 2 cut(s) 150, 365
SacI GAGCTC 1 cut(s) 692
SalI GTCGAC 1 cut(s) 602
SapI GCTCTTC 1 cut(s) 201
SaqAI TTAA 4 cut(s) 344, 530, 672, 948
SatI GCNGC 1 cut(s) 595
Sau3AI GATC 6 cut(s) 232, 257, 471, 480, 487, 888
Sau96I GGNCC 3 cut(s) 115, 310, 357
SchI GAGTC 1 cut(s) 244
ScrFI CCNGG 3 cut(s) 150, 336, 599
SduI GDGCHC 3 cut(s) 147, 565, 692
SfaNI GCATC 1 cut(s) 817
SfcI CTRYAG 3 cut(s) 556, 615, 705
SinI GGWCC 1 cut(s) 357
SmiMI CAYNNNNRTG 2 cut(s) 150, 365
SmlI CTYRAG 2 cut(s) 476, 947
SmoI CTYRAG 2 cut(s) 476, 947
Sse9I AATT 6 cut(s) 5, 137, 570, 754, 813, 902
SsiI CCGC 4 cut(s) 14, 98, 519, 595
SspI AATATT 1 cut(s) 342
SstI GAGCTC 1 cut(s) 692
StyD4I CCNGG 3 cut(s) 148, 334, 597
TaaI ACNGT 4 cut(s) 34, 124, 526, 967
TaiI ACGT 2 cut(s) 246, 670
TaqI TCGA 6 cut(s) 141, 184, 240, 288, 427, 603
TasI AATT 6 cut(s) 5, 137, 570, 754, 813, 902
TauI GCSGC 1 cut(s) 597
TfiI GAWTC 3 cut(s) 178, 211, 760
Tru1I TTAA 4 cut(s) 344, 530, 672, 948
Tru9I TTAA 4 cut(s) 344, 530, 672, 948
TscAI CASTG 1 cut(s) 734
TspDTI ATGAA 4 cut(s) 317, 474, 915, 936
TspGWI ACGGA 4 cut(s) 96, 370, 734, 925
TspRI CASTG 1 cut(s) 734
Tth111I GACNNNGTC 1 cut(s) 542
Van91I CCANNNNNTGG 2 cut(s) 124, 155
Vha464I CTTAAG 1 cut(s) 947
VpaK11BI GGWCC 1 cut(s) 357
VspI ATTAAT 1 cut(s) 344
XapI RAATTY 3 cut(s) 137, 570, 902
XceI RCATGY 1 cut(s) 610
XmiI GTMKAC 1 cut(s) 603
XmnI GAANNNNTTC 1 cut(s) 137
Zsp2I ATGCAT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.